Rw5G042530

Domain of unknown function (DUF4792)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001521
Physical Location & Seq
Forward (+)
74455608 .. 74458759
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042530.1

Sequence Viewer

Length: 1377 bp
ATGGAAGACCCAGAAACAGATCGTAGCCACCATGAATCCGTTCCTTCTTCTTCTTCGTCTTCTTCTTCTTCTTCATCATCTACCTCACAGGCCCAAGAACAAACAGAGCAGCAACCAGAGCAGAGAAGTTCAACTAGGGCTCTCTCTTACCGTGTCAATGTATTGATAGCTGATGTAGCAGCATTTGACATGAAGGATGATCTTTGGTCTGGCTTTATTGTGCTCGTCACCTTTTGGTTCTTTGCATCCATGACTTTGATTCTTGGTTTCTTTGGATCCGCCGATTTACAACTGGGCCCGAATTGCTCACGTCTCATACGAACTAATCCATTCTTTGTTCAGACCATTAAGGCACAGGAACTTCAGGGTACAAAACCCGGGTCAATTTTATATGGATTCCACACACCTCCTCCTCTGGATGTTGAAACAGCTTGGACTGAAACACATCATACACTTGTGCACCCTACTTATCACAAGGAATGGATTTACTTTCTAAACAAAGGGTCCAGAATAGATATATATTACCATGTCAAACCTTCAAGTTCCTCACCTTTAACACTTGTAATTGCAGAAGGTAGAGAAAGCCTGGTTGAATGGATAGAGGATCCATCATATGGTAATGCAACGTTGTCTTGGAATATAATTTATGGAAGTGGTAAGATCCAACAGGTAATTTCAGAGTCTTCTAATTATTATATTGCAGTAGGGAACTTGAACCCTGAGGATGTTGAGGTAGAGTTGGAATTCAACATAAAGTCCACTCTTTATAACACGGCCCAGGCGTATTACAGGTGCTCCATACATAATCAATACTGTAGCTTAAAGCTTTCCCTCGTAGGGGCAACTTTTGCTGTCTTAACTTCTCCAGGTCCTGAAGAGGGTATTCGGGAGGATGACTGGTATGTCAAACTGTCATATGGACCTCGATGGGTCATCTATTTTCTTGGATCAGGTGTGATGACTGTGCTCCTGTTAATGGTCTTTAGATTTTGTAACATTTTCCAAAATGCTGGTGAGGATAGAACAGATTTTCAAGCAAGGGAGGCATCACCTGTACGAACCCCGTTGCTTTTACCCAAAGATGATGATGCTTTGAGTTGGGGTTCATCTTATGAGTGTATTTCAAATGATGAAGAGGATTTGGAAGAGTTCCTTGGAGTGAGTTCCGTTGAAGGAAAGTCTATAAATGAAAGGAACGACAACAATACTCAGCGTCTTTGTGTTATTTGCTTTGATGGGCCGAGAGACTGCTTCTTTCTTCCATGTGGGCACTGTGCTACCTGTTTTACTTGTGGAACAAGGATTTCTGAAGAGGCTGGAACTTGTCCCATTTGTCGTATGAAGATGAAGAAAGTGAGGAAGATTTTTTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

458

Amino Acids

51.51

Weight (kDa)

4.98

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 127 - 196 1.3e-21 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 221 - 327 1e-32 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 404 - 452 4.9e-13 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 406 - 446 6.1e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AasI GACNNNNNNGTC 1 cut(s) 902
AccB7I CCANNNNNTGG 1 cut(s) 614
AciI CCGC 1 cut(s) 279
AclI AACGTT 1 cut(s) 626
AclWI GGATC 6 cut(s) 270, 283, 599, 612, 655, 955
AcsI RAATTY 1 cut(s) 743
AcuI CTGAAG 3 cut(s) 347, 894, 1329
AfaI GTAC 2 cut(s) 370, 1056
AfiI CCNNNNNNNGG 5 cut(s) 614, 837, 838, 878, 976
AgsI TTSAA 9 cut(s) 132, 425, 540, 593, 715, 748, 1034, 1125, 1172
AjiI CACGTC 1 cut(s) 311
AjnI CCWGG 3 cut(s) 585, 777, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 1137, 1169
AluBI AGCT 4 cut(s) 170, 431, 819, 826
AluI AGCT 4 cut(s) 170, 431, 819, 826
Alw21I GWGCWC 4 cut(s) 225, 462, 797, 969
Alw26I GTCTC 2 cut(s) 317, 1239
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 6 cut(s) 270, 283, 599, 612, 655, 955
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 4 cut(s) 90, 295, 774, 1238
ApaI GGGCCC 1 cut(s) 299
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 2 cut(s) 109, 179
ApoI RAATTY 1 cut(s) 743
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
Asp700I GAANNNNTTC 1 cut(s) 39
AspS9I GGNCC 8 cut(s) 91, 295, 296, 504, 775, 869, 920, 1238
AsuC2I CCSGG 2 cut(s) 378, 379
AsuHPI GGTGA 4 cut(s) 220, 540, 1025, 1041
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 504, 869, 920
AxyI CCTNAGG 1 cut(s) 720
BaeGI GKGCMC 3 cut(s) 299, 462, 1272
BamHI GGATCC 2 cut(s) 275, 604
BanII GRGCYC 2 cut(s) 142, 299
BbsI GAAGAC 3 cut(s) 12, 51, 675
Bbv12I GWGCWC 4 cut(s) 225, 462, 797, 969
BbvI GCAGC 2 cut(s) 121, 191
BccI CCATC 3 cut(s) 616, 921, 1229
BceAI ACGGC 1 cut(s) 789
BciT130I CCWGG 3 cut(s) 587, 779, 867
BcnI CCSGG 2 cut(s) 378, 379
BcoDI GTCTC 2 cut(s) 317, 1239
BfaI CTAG 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 814
BisI GCNGC 2 cut(s) 110, 180
BlsI GCNGC 2 cut(s) 111, 181
Bme1390I CCNGG 5 cut(s) 378, 379, 587, 779, 867
Bme18I GGWCC 3 cut(s) 504, 869, 920
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 8 cut(s) 91, 295, 296, 504, 775, 869, 920, 1238
BmiI GGNNCC 4 cut(s) 277, 297, 505, 606
BmrFI CCNGG 5 cut(s) 378, 379, 587, 779, 867
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 3 cut(s) 254, 1055, 1078
BmuI ACTGGG 1 cut(s) 302
BpiI GAAGAC 3 cut(s) 12, 51, 675
BpmI CTGGAG 1 cut(s) 849
BpuMI CCSGG 2 cut(s) 378, 379
BsaJI CCNNGG 3 cut(s) 377, 777, 1153
BsaXI ACNNNNNCTCC 4 cut(s) 394, 424, 779, 809
Bsc4I CCNNNNNNNGG 5 cut(s) 614, 837, 838, 878, 976
Bse1I ACTGG 2 cut(s) 297, 902
Bse21I CCTNAGG 1 cut(s) 720
BseBI CCWGG 3 cut(s) 587, 779, 867
BseDI CCNNGG 3 cut(s) 377, 777, 1153
BseGI GGATG 5 cut(s) 202, 245, 424, 730, 898
BseLI CCNNNNNNNGG 5 cut(s) 614, 837, 838, 878, 976
BseMII CTCAG 2 cut(s) 711, 1223
BseNI ACTGG 2 cut(s) 297, 902
BseRI GAGGAG 2 cut(s) 399, 402
BseSI GKGCMC 3 cut(s) 299, 462, 1272
BseXI GCAGC 2 cut(s) 121, 191
BshFI GGCC 4 cut(s) 92, 297, 776, 1240
BsiHKAI GWGCWC 4 cut(s) 225, 462, 797, 969
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 1311
BslI CCNNNNNNNGG 5 cut(s) 614, 837, 838, 878, 976
BsmAI GTCTC 2 cut(s) 317, 1239
BsmBI CGTCTC 1 cut(s) 317
BsmFI GGGAC 1 cut(s) 1311
BsnI GGCC 4 cut(s) 92, 297, 776, 1240
BsoBI CYCGRG 1 cut(s) 377
Bsp120I GGGCCC 1 cut(s) 295
Bsp1286I GDGCHC 7 cut(s) 142, 225, 299, 462, 797, 969, 1272
Bsp143I GATC 6 cut(s) 19, 199, 275, 604, 660, 947
BspACI CCGC 1 cut(s) 279
BspANI GGCC 4 cut(s) 92, 297, 776, 1240
BspCNI CTCAG 2 cut(s) 712, 1222
BspLI GGNNCC 4 cut(s) 277, 297, 505, 606
BspPI GGATC 6 cut(s) 270, 283, 599, 612, 655, 955
BsrI ACTGG 2 cut(s) 297, 902
BssECI CCNNGG 3 cut(s) 377, 777, 1153
BssMI GATC 6 cut(s) 19, 199, 275, 604, 660, 947
BssT1I CCWWGG 1 cut(s) 1153
Bst2UI CCWGG 3 cut(s) 587, 779, 867
Bst4CI ACNGT 5 cut(s) 152, 815, 912, 964, 1274
Bst6I CTCTTC 4 cut(s) 870, 1128, 1140, 1305
BstAPI GCANNNNNTGC 1 cut(s) 848
BstDEI CTNAG 2 cut(s) 720, 1209
BstF5I GGATG 5 cut(s) 202, 245, 424, 730, 898
BstKTI GATC 6 cut(s) 22, 202, 278, 607, 663, 950
BstMAI GTCTC 2 cut(s) 317, 1239
BstMBI GATC 6 cut(s) 19, 199, 275, 604, 660, 947
BstMWI GCNNNNNNNGC 5 cut(s) 118, 176, 303, 848, 1043
BstNI CCWGG 3 cut(s) 587, 779, 867
BstSCI CCNGG 5 cut(s) 376, 377, 585, 777, 865
BstSFI CTRYAG 1 cut(s) 814
BstSLI GKGCMC 3 cut(s) 299, 462, 1272
BstV1I GCAGC 2 cut(s) 121, 191
BstV2I GAAGAC 3 cut(s) 12, 51, 675
BstX2I RGATCY 3 cut(s) 275, 604, 660
BstXI CCANNNNNNTGG 1 cut(s) 1010
BstYI RGATCY 3 cut(s) 275, 604, 660
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 4 cut(s) 92, 297, 776, 1240
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 5 cut(s) 202, 245, 424, 730, 898
BtsIMutI CAGTG 1 cut(s) 1270
CaiI CAGNNNCTG 1 cut(s) 872
Cfr13I GGNCC 8 cut(s) 91, 295, 296, 504, 775, 869, 920, 1238
Cfr9I CCCGGG 1 cut(s) 377
CseI GACGC 1 cut(s) 1202
Csp6I GTAC 2 cut(s) 369, 1055
CviAII CATG 5 cut(s) 32, 190, 250, 527, 1263
CviQI GTAC 2 cut(s) 369, 1055
DdeI CTNAG 2 cut(s) 720, 1209
DpnI GATC 6 cut(s) 21, 201, 277, 606, 662, 949
DpnII GATC 6 cut(s) 19, 199, 275, 604, 660, 947
DrdI GACNNNNNNGTC 1 cut(s) 902
DseDI GACNNNNNNGTC 1 cut(s) 902
Eam1104I CTCTTC 4 cut(s) 870, 1128, 1140, 1305
EarI CTCTTC 4 cut(s) 870, 1128, 1140, 1305
EciI GGCGGA 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 1153
Eco24I GRGCYC 2 cut(s) 142, 299
Eco47I GGWCC 3 cut(s) 504, 869, 920
Eco57I CTGAAG 3 cut(s) 347, 894, 1329
Eco81I CCTNAGG 1 cut(s) 720
Eco88I CYCGRG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 743
EcoRII CCWGG 3 cut(s) 585, 777, 865
EcoT14I CCWWGG 1 cut(s) 1153
EcoT38I GRGCYC 2 cut(s) 142, 299
ErhI CCWWGG 1 cut(s) 1153
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 5 cut(s) 35, 193, 253, 530, 1266
FalI AAGNNNNNCTT 2 cut(s) 1137, 1169
FaqI GGGAC 1 cut(s) 1311
FatI CATG 5 cut(s) 31, 189, 249, 526, 1262
FauNDI CATATG 2 cut(s) 613, 916
Fnu4HI GCNGC 2 cut(s) 110, 180
FokI GGATG 5 cut(s) 209, 232, 431, 737, 905
FriOI GRGCYC 2 cut(s) 142, 299
Fsp4HI GCNGC 2 cut(s) 110, 180
FspBI CTAG 1 cut(s) 135
GluI GCNGC 2 cut(s) 110, 180
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 4 cut(s) 92, 297, 776, 1240
HapII CCGG 1 cut(s) 378
HgaI GACGC 1 cut(s) 1202
Hin1II CATG 5 cut(s) 35, 193, 253, 530, 1266
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 4 cut(s) 35, 259, 396, 680
HpaII CCGG 1 cut(s) 378
HphI GGTGA 4 cut(s) 220, 540, 1025, 1041
Hpy166II GTNNAC 2 cut(s) 460, 759
Hpy188I TCNGA 3 cut(s) 342, 679, 1309
Hpy188III TCNNGA 4 cut(s) 416, 507, 872, 887
Hpy8I GTNNAC 2 cut(s) 460, 759
HpyAV CCTTC 5 cut(s) 54, 187, 546, 566, 1166
HpyCH4III ACNGT 5 cut(s) 152, 815, 912, 964, 1274
HpyCH4IV ACGT 2 cut(s) 310, 626
HpyCH4V TGCA 5 cut(s) 245, 460, 569, 623, 701
HpyF10VI GCNNNNNNNGC 5 cut(s) 118, 176, 303, 848, 1043
HpyF3I CTNAG 2 cut(s) 720, 1209
HpySE526I ACGT 2 cut(s) 310, 626
Hsp92II CATG 5 cut(s) 35, 193, 253, 530, 1266
Kzo9I GATC 6 cut(s) 19, 199, 275, 604, 660, 947
LmnI GCTCC 2 cut(s) 800, 972
Lsp1109I GCAGC 2 cut(s) 121, 191
LweI GCATC 3 cut(s) 254, 1055, 1078
MaeI CTAG 1 cut(s) 135
MaeII ACGT 2 cut(s) 310, 626
MaeIII GTNAC 2 cut(s) 226, 992
MalI GATC 6 cut(s) 21, 201, 277, 606, 662, 949
MboI GATC 6 cut(s) 19, 199, 275, 604, 660, 947
MflI RGATCY 3 cut(s) 275, 604, 660
MhlI GDGCHC 7 cut(s) 142, 225, 299, 462, 797, 969, 1272
MluCI AATT 7 cut(s) 301, 384, 564, 642, 672, 688, 743
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 2 cut(s) 688, 720
MroXI GAANNNNTTC 1 cut(s) 39
MseI TTAA 5 cut(s) 348, 554, 821, 857, 974
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 5 cut(s) 378, 379, 587, 779, 867
MvaI CCWGG 3 cut(s) 587, 779, 867
MwoI GCNNNNNNNGC 5 cut(s) 118, 176, 303, 848, 1043
NciI CCSGG 2 cut(s) 378, 379
NdeI CATATG 2 cut(s) 613, 916
NdeII GATC 6 cut(s) 19, 199, 275, 604, 660, 947
NlaIII CATG 5 cut(s) 35, 193, 253, 530, 1266
NlaIV GGNNCC 4 cut(s) 277, 297, 505, 606
NmeAIII GCCGAG 1 cut(s) 1266
NmuCI GTSAC 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 316, 779
PdmI GAANNNNTTC 1 cut(s) 39
PfeI GAWTC 3 cut(s) 35, 259, 396
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 2 cut(s) 111, 181
PleI GAGTC 1 cut(s) 688
PpsI GAGTC 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 869
PsiI TTATAA 1 cut(s) 768
Psp1406I AACGTT 1 cut(s) 626
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 3 cut(s) 585, 777, 865
PspGI CCWGG 3 cut(s) 585, 777, 865
PspN4I GGNNCC 4 cut(s) 277, 297, 505, 606
PspOMI GGGCCC 1 cut(s) 295
PspPI GGNCC 8 cut(s) 91, 295, 296, 504, 775, 869, 920, 1238
PspPPI RGGWCCY 1 cut(s) 869
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 3 cut(s) 275, 604, 660
RsaI GTAC 2 cut(s) 370, 1056
RsaNI GTAC 2 cut(s) 369, 1055
SaqAI TTAA 5 cut(s) 348, 554, 821, 857, 974
SatI GCNGC 2 cut(s) 110, 180
Sau3AI GATC 6 cut(s) 19, 199, 275, 604, 660, 947
Sau96I GGNCC 8 cut(s) 91, 295, 296, 504, 775, 869, 920, 1238
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 5 cut(s) 378, 379, 587, 779, 867
SduI GDGCHC 7 cut(s) 142, 225, 299, 462, 797, 969, 1272
SfaNI GCATC 3 cut(s) 254, 1055, 1078
SfcI CTRYAG 1 cut(s) 814
SinI GGWCC 3 cut(s) 504, 869, 920
SmaI CCCGGG 1 cut(s) 379
Sse9I AATT 7 cut(s) 301, 384, 564, 642, 672, 688, 743
SsiI CCGC 1 cut(s) 279
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 376, 377, 585, 777, 865
StyI CCWWGG 1 cut(s) 1153
TaaI ACNGT 5 cut(s) 152, 815, 912, 964, 1274
TaiI ACGT 2 cut(s) 313, 629
TaqI TCGA 1 cut(s) 925
TasI AATT 7 cut(s) 301, 384, 564, 642, 672, 688, 743
TfiI GAWTC 3 cut(s) 35, 259, 396
Tru1I TTAA 5 cut(s) 348, 554, 821, 857, 974
Tru9I TTAA 5 cut(s) 348, 554, 821, 857, 974
TscAI CASTG 1 cut(s) 1277
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 109, 179
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 8 cut(s) 48, 63, 206, 1095, 1146, 1203, 1355, 1361
TspGWI ACGGA 2 cut(s) 28, 1156
TspMI CCCGGG 1 cut(s) 377
TspRI CASTG 1 cut(s) 1277
Van91I CCANNNNNTGG 1 cut(s) 614
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 3 cut(s) 504, 869, 920
XapI RAATTY 1 cut(s) 743
XmaI CCCGGG 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 39
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.