pycom03g05570

Domain of unknown function (DUF4792)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001521
Physical Location & Seq
Reverse (-)
4416530 .. 4419576
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05570.5

Sequence Viewer

Length: 1242 bp
ATGAGAAGGTCTAGGACTTATCATGTGAACTTGTTGGTTTCCGATGTAGAAGTCTCTGAGGATGATATTTGGTCGGCCCTTGCTGTGCTTGTCACCTTTTGGTTCTTTGCATCTATGACTATGATTCTTGGTTTCTATGGATCTGGCAATCTACAATTGGGCCCGAACTGCTCACGCTTGATACAAACTAATCCGTTCTTTGTGCAATCGATTAAGGCACAGGAGCTTGATGTGCAAAAACTTGGCCCAGTGTTATATGGATTCAACAAACCTCCTCCCTTGGATGTTGAAATAGCATGGACCGAAACACAGGATATGCTTGTTCCAGCCAATTTTCACAAGGCATGGATTTACTTTCTAAACAAAGGGTCTGGTTTGGATATTACTTACCGTGTGAAGCCTACAAGTTCCTCGCCTTTAACTCTTGTAATCGCACAAGGTAGTGAGAGCCTGGCTGAATGGATAGAGGATCCGTCATACGGTAATGCAACATTGTCTTGGAATATAATTTATGGAAGTGGTAGGATCCAGCAGGAAATTCCAAAGTCTTCTACTTATTATATCGCAGTACGAAACTTGAACCCTGAGTTTGTTGAGGTAGAGTTGGTATTCAACATAAAATCTGTTCTTTACAACACAACCGAGGCGTATTATAAATGCTCTTTGTATAACCGGTTATGTAGCTTAAAGCTTTCCCTCTTGGGGGCAAATGTTGCTGTCATAACTTCTCCAGGTCCTAAAGAGGGTATCCCTAATGATGATTGGTATGTAAGCCTGTCTTATGGACCGCGATGGGCCATGTATTTTCTTGTAGCAGGCGTTGTGACTTTGCTCCTCGTAAAGGCCTTTAAATTTTATAACAAATTCCAGAATTCTAGTGAAGACACAACCGAATTTCAAGCAGAGGAGGGACAATCTGTGCAAACCCCGCTGCTTTCACCCAAAGACGATGATACTTTGAGTTGGGGTTCGTCGTATGAATTTATTTCCAATAATGAAGAAGATTTGGATGAGTGCCTTGCAGTGAGTTCCCTTGAAGGAAGGTTAACAAATGAAGGAGATAGCAACAATACTCGGCATCTTTGTGTCATATGCTTTGATGGTCCTAGGGATTGCTTCTTTCTTCCCTGTGGGCATTGCGCTGCCTGTTTTACTTGCGGAACAAGGATTGCCGAGGAGGCTGGTACGTGTCCCATATGTCGTAGAAGGATGAAGAAAGTGAGGAGGATTTTTACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

414

Amino Acids

46.44

Weight (kDa)

5.09

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 654, 858
AccII CGCG 1 cut(s) 790
AciI CCGC 3 cut(s) 788, 929, 1158
AclWI GGATC 5 cut(s) 148, 464, 477, 520, 533
AcsI RAATTY 6 cut(s) 537, 851, 863, 871, 893, 980
AfaI GTAC 2 cut(s) 570, 1186
AfiI CCNNNNNNNGG 5 cut(s) 479, 702, 703, 743, 841
AflIII ACRYGT 1 cut(s) 1187
AgeI ACCGGT 1 cut(s) 672
AgsI TTSAA 6 cut(s) 265, 290, 580, 613, 899, 1037
AjnI CCWGG 2 cut(s) 450, 730
AluBI AGCT 3 cut(s) 226, 684, 691
AluI AGCT 3 cut(s) 226, 684, 691
Alw26I GTCTC 1 cut(s) 58
AlwI GGATC 5 cut(s) 148, 464, 477, 520, 533
AoxI GGCC 5 cut(s) 75, 160, 244, 795, 843
ApaI GGGCCC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 931, 1142
ApoI RAATTY 6 cut(s) 537, 851, 863, 871, 893, 980
AsiGI ACCGGT 1 cut(s) 672
AspA2I CCTAGG 1 cut(s) 1106
AspLEI GCGC 1 cut(s) 1142
AspS9I GGNCC 9 cut(s) 76, 160, 161, 245, 300, 734, 785, 795, 1103
AsuHPI GGTGA 2 cut(s) 85, 930
AvaII GGWCC 4 cut(s) 300, 734, 785, 1103
AvrII CCTAGG 1 cut(s) 1106
BaeGI GKGCMC 1 cut(s) 164
BamHI GGATCC 2 cut(s) 469, 525
BanII GRGCYC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 540, 888
BbvI GCAGC 2 cut(s) 918, 1129
BccI CCATC 2 cut(s) 786, 1094
BciT130I CCWGG 2 cut(s) 452, 732
BciVI GTATCC 1 cut(s) 758
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 3 cut(s) 12, 876, 1107
BfuI GTATCC 1 cut(s) 758
BglI GCCNNNNNGGC 1 cut(s) 1178
BisI GCNGC 2 cut(s) 932, 1143
BlnI CCTAGG 1 cut(s) 1106
BlsI GCNGC 2 cut(s) 933, 1144
Bme1390I CCNGG 2 cut(s) 452, 732
Bme18I GGWCC 4 cut(s) 300, 734, 785, 1103
BmgT120I GGNCC 9 cut(s) 76, 160, 161, 245, 300, 734, 785, 795, 1103
BmiI GGNNCC 3 cut(s) 162, 471, 527
BmrFI CCNGG 2 cut(s) 452, 732
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 119, 1087
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 2 cut(s) 540, 888
BpmI CTGGAG 1 cut(s) 714
Bsa29I ATCGAT 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 1188
BsaJI CCNNGG 4 cut(s) 279, 642, 1106, 1173
BsaWI WCCGGW 1 cut(s) 672
Bsc4I CCNNNNNNNGG 5 cut(s) 479, 702, 703, 743, 841
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 1135
BseBI CCWGG 2 cut(s) 452, 732
BseCI ATCGAT 1 cut(s) 209
BseDI CCNNGG 4 cut(s) 279, 642, 1106, 1173
BseGI GGATG 4 cut(s) 67, 289, 1015, 1215
BseLI CCNNNNNNNGG 5 cut(s) 479, 702, 703, 743, 841
BseMI GCAATG 1 cut(s) 1135
BseMII CTCAG 2 cut(s) 48, 576
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 5 cut(s) 264, 824, 920, 1190, 1237
BseSI GKGCMC 1 cut(s) 164
BseXI GCAGC 2 cut(s) 918, 1129
Bsh1236I CGCG 1 cut(s) 790
BshFI GGCC 5 cut(s) 77, 162, 246, 797, 845
BshTI ACCGGT 1 cut(s) 672
BshVI ATCGAT 1 cut(s) 209
BsiSI CCGG 1 cut(s) 673
BslFI GGGAC 2 cut(s) 924, 1176
BslI CCNNNNNNNGG 5 cut(s) 479, 702, 703, 743, 841
BsmAI GTCTC 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 924, 1176
BsnI GGCC 5 cut(s) 77, 162, 246, 797, 845
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 3 cut(s) 140, 469, 525
BspACI CCGC 3 cut(s) 788, 929, 1158
BspANI GGCC 5 cut(s) 77, 162, 246, 797, 845
BspCNI CTCAG 2 cut(s) 49, 577
BspDI ATCGAT 1 cut(s) 209
BspFNI CGCG 1 cut(s) 790
BspLI GGNNCC 3 cut(s) 162, 471, 527
BspPI GGATC 5 cut(s) 148, 464, 477, 520, 533
BsrDI GCAATG 1 cut(s) 1135
BsrFI RCCGGY 1 cut(s) 672
BsrI ACTGG 1 cut(s) 248
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 4 cut(s) 279, 642, 1106, 1173
BssMI GATC 3 cut(s) 140, 469, 525
BssT1I CCWWGG 2 cut(s) 279, 1106
Bst2UI CCWGG 2 cut(s) 452, 732
Bst4CI ACNGT 3 cut(s) 392, 482, 1237
BstAPI GCANNNNNTGC 1 cut(s) 713
BstBAI YACGTR 1 cut(s) 1188
BstC8I GCNNGC 1 cut(s) 817
BstDEI CTNAG 2 cut(s) 57, 585
BstENI CCTNNNNNAGG 1 cut(s) 839
BstF5I GGATG 4 cut(s) 67, 289, 1015, 1215
BstFNI CGCG 1 cut(s) 790
BstHHI GCGC 1 cut(s) 1142
BstKTI GATC 3 cut(s) 143, 472, 528
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 3 cut(s) 140, 469, 525
BstMWI GCNNNNNNNGC 5 cut(s) 168, 232, 713, 928, 1178
BstNI CCWGG 2 cut(s) 452, 732
BstSCI CCNGG 2 cut(s) 450, 730
BstSLI GKGCMC 1 cut(s) 164
BstUI CGCG 1 cut(s) 790
BstV1I GCAGC 2 cut(s) 918, 1129
BstV2I GAAGAC 2 cut(s) 540, 888
BstX2I RGATCY 3 cut(s) 140, 469, 525
BstYI RGATCY 3 cut(s) 140, 469, 525
Bsu15I ATCGAT 1 cut(s) 209
BsuI GTATCC 1 cut(s) 758
BsuRI GGCC 5 cut(s) 77, 162, 246, 797, 845
BsuTUI ATCGAT 1 cut(s) 209
BtgZI GCGATG 1 cut(s) 805
BtsCI GGATG 4 cut(s) 67, 289, 1015, 1215
BtsI GCAGTG 1 cut(s) 1029
BtsIMutI CAGTG 2 cut(s) 255, 1029
Cac8I GCNNGC 1 cut(s) 817
CfoI GCGC 1 cut(s) 1142
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 9 cut(s) 76, 160, 161, 245, 300, 734, 785, 795, 1103
ClaI ATCGAT 1 cut(s) 209
Csp6I GTAC 2 cut(s) 569, 1185
CspAI ACCGGT 1 cut(s) 672
CviAII CATG 4 cut(s) 23, 297, 345, 799
CviQI GTAC 2 cut(s) 569, 1185
DdeI CTNAG 2 cut(s) 57, 585
DpnI GATC 3 cut(s) 142, 471, 527
DpnII GATC 3 cut(s) 140, 469, 525
DraI TTTAAA 1 cut(s) 850
Eco130I CCWWGG 2 cut(s) 279, 1106
Eco147I AGGCCT 1 cut(s) 845
Eco24I GRGCYC 1 cut(s) 164
Eco47I GGWCC 4 cut(s) 300, 734, 785, 1103
EcoNI CCTNNNNNAGG 1 cut(s) 839
EcoO109I RGGNCCY 1 cut(s) 734
EcoRI GAATTC 1 cut(s) 871
EcoRII CCWGG 2 cut(s) 450, 730
EcoT14I CCWWGG 2 cut(s) 279, 1106
EcoT38I GRGCYC 1 cut(s) 164
ErhI CCWWGG 2 cut(s) 279, 1106
FaeI CATG 4 cut(s) 26, 300, 348, 802
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 2 cut(s) 924, 1176
FatI CATG 4 cut(s) 22, 296, 344, 798
FauI CCCGC 1 cut(s) 936
FauNDI CATATG 2 cut(s) 1091, 1196
Fnu4HI GCNGC 2 cut(s) 932, 1143
FokI GGATG 4 cut(s) 74, 296, 1022, 1222
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 932, 1143
FspBI CTAG 3 cut(s) 12, 876, 1107
GlaI GCGC 1 cut(s) 1141
GluI GCNGC 2 cut(s) 932, 1143
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 5 cut(s) 77, 162, 246, 797, 845
HapII CCGG 1 cut(s) 673
HhaI GCGC 1 cut(s) 1142
Hin1II CATG 4 cut(s) 26, 300, 348, 802
Hin6I GCGC 1 cut(s) 1140
HinP1I GCGC 1 cut(s) 1140
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 2 cut(s) 124, 261
HpaI GTTAAC 1 cut(s) 1047
HpaII CCGG 1 cut(s) 673
HphI GGTGA 2 cut(s) 85, 930
Hpy166II GTNNAC 2 cut(s) 28, 1047
Hpy188I TCNGA 2 cut(s) 43, 58
Hpy188III TCNNGA 1 cut(s) 868
Hpy8I GTNNAC 2 cut(s) 28, 1047
Hpy99I CGWCG 1 cut(s) 976
HpyAV CCTTC 4 cut(s) 1031, 1035, 1049, 1200
HpyCH4III ACNGT 3 cut(s) 392, 482, 1237
HpyCH4IV ACGT 1 cut(s) 1187
HpyCH4V TGCA 6 cut(s) 110, 205, 235, 488, 922, 1022
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 232, 713, 928, 1178
HpyF3I CTNAG 2 cut(s) 57, 585
HpySE526I ACGT 1 cut(s) 1187
Hsp92II CATG 4 cut(s) 26, 300, 348, 802
HspAI GCGC 1 cut(s) 1140
KspAI GTTAAC 1 cut(s) 1047
Kzo9I GATC 3 cut(s) 140, 469, 525
LmnI GCTCC 2 cut(s) 223, 837
Lsp1109I GCAGC 2 cut(s) 918, 1129
LweI GCATC 2 cut(s) 119, 1087
MaeI CTAG 3 cut(s) 12, 876, 1107
MaeII ACGT 1 cut(s) 1187
MaeIII GTNAC 2 cut(s) 91, 823
MalI GATC 3 cut(s) 142, 471, 527
MboI GATC 3 cut(s) 140, 469, 525
MboII GAAGA 6 cut(s) 540, 893, 1010, 1013, 1115, 1225
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 3 cut(s) 140, 469, 525
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 9 cut(s) 155, 331, 507, 537, 851, 863, 871, 893, 980
MseI TTAA 5 cut(s) 213, 419, 686, 849, 1046
MslI CAYNNNNRTG 1 cut(s) 1083
MspA1I CMGCKG 1 cut(s) 931
MspI CCGG 1 cut(s) 673
MspR9I CCNGG 2 cut(s) 452, 732
MunI CAATTG 1 cut(s) 155
MvaI CCWGG 2 cut(s) 452, 732
MvnI CGCG 1 cut(s) 790
MwoI GCNNNNNNNGC 5 cut(s) 168, 232, 713, 928, 1178
NdeI CATATG 2 cut(s) 1091, 1196
NdeII GATC 3 cut(s) 140, 469, 525
NlaIII CATG 4 cut(s) 26, 300, 348, 802
NlaIV GGNNCC 3 cut(s) 162, 471, 527
NmeAIII GCCGAG 2 cut(s) 1054, 1198
NmuCI GTSAC 2 cut(s) 91, 823
PceI AGGCCT 1 cut(s) 845
PfeI GAWTC 2 cut(s) 124, 261
PinAI ACCGGT 1 cut(s) 672
PkrI GCNGC 2 cut(s) 933, 1144
Ppu21I YACGTR 1 cut(s) 1188
PpuMI RGGWCCY 1 cut(s) 734
PsiI TTATAA 2 cut(s) 654, 858
Psp5II RGGWCCY 1 cut(s) 734
Psp6I CCWGG 2 cut(s) 450, 730
PspGI CCWGG 2 cut(s) 450, 730
PspN4I GGNNCC 3 cut(s) 162, 471, 527
PspOMI GGGCCC 1 cut(s) 160
PspPI GGNCC 9 cut(s) 76, 160, 161, 245, 300, 734, 785, 795, 1103
PspPPI RGGWCCY 1 cut(s) 734
PsuI RGATCY 3 cut(s) 140, 469, 525
RsaI GTAC 2 cut(s) 570, 1186
RsaNI GTAC 2 cut(s) 569, 1185
RseI CAYNNNNRTG 1 cut(s) 1083
SaqAI TTAA 5 cut(s) 213, 419, 686, 849, 1046
SatI GCNGC 2 cut(s) 932, 1143
Sau3AI GATC 3 cut(s) 140, 469, 525
Sau96I GGNCC 9 cut(s) 76, 160, 161, 245, 300, 734, 785, 795, 1103
ScrFI CCNGG 2 cut(s) 452, 732
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 119, 1087
SinI GGWCC 4 cut(s) 300, 734, 785, 1103
SmiMI CAYNNNNRTG 1 cut(s) 1083
Sse9I AATT 9 cut(s) 155, 331, 507, 537, 851, 863, 871, 893, 980
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 3 cut(s) 788, 929, 1158
SspMI CTAG 3 cut(s) 12, 876, 1107
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 2 cut(s) 450, 730
StyI CCWWGG 2 cut(s) 279, 1106
TaaI ACNGT 3 cut(s) 392, 482, 1237
TaiI ACGT 1 cut(s) 1190
TaqI TCGA 1 cut(s) 209
TaqII GACCGA 1 cut(s) 317
TasI AATT 9 cut(s) 155, 331, 507, 537, 851, 863, 871, 893, 980
TfiI GAWTC 2 cut(s) 124, 261
Tru1I TTAA 5 cut(s) 213, 419, 686, 849, 1046
Tru9I TTAA 5 cut(s) 213, 419, 686, 849, 1046
TscAI CASTG 2 cut(s) 255, 1029
TseFI GTSAC 2 cut(s) 91, 823
TseI GCWGC 2 cut(s) 931, 1142
Tsp45I GTSAC 2 cut(s) 91, 823
TspDTI ATGAA 4 cut(s) 993, 1011, 1068, 1226
TspGWI ACGGA 2 cut(s) 183, 462
TspRI CASTG 2 cut(s) 255, 1029
VpaK11BI GGWCC 4 cut(s) 300, 734, 785, 1103
XagI CCTNNNNNAGG 1 cut(s) 839
XapI RAATTY 6 cut(s) 537, 851, 863, 871, 893, 980
XmaJI CCTAGG 1 cut(s) 1106
XspI CTAG 3 cut(s) 12, 876, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.