Rh5DG486300

Domain of unknown function (DUF4792)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001521
Physical Location & Seq
Forward (+)
76509866 .. 76513413
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG486300.1

Sequence Viewer

Length: 1128 bp
ATGACTTTGATTCTTGGTTTCTTTGGATCCGCCGATTTACAACTGGGCCCGAATTGCTCACGTCTCATACGAACTAATCCGTTCTTTGTTCAGACCATTAAGGCACAGGAAATTGAGGGTACAAAACCCGGGTCAATTTTATATGGATTCCACACACCTCCTCCTCTGGATGTTGAAATAGCTTGGACTGAAACACATCATACTCTTGTGCACCCTACTTATCACAAGGAATGGATTTACTTTCTAAACAAAGGGTCTAGAATAGATATATATTACCATGTCAAACCTTCAAGTTCCTCGCCCTTAACACTTGTAATTGCAGAAGGTAGAAAAAGCCTGGTTGAATGGATAGAGGATCCATCATATGGTAATGCAACATTGTCTTGGAATATAATTTATGGAAGTGGTAAGATCCAACAGGTAATTTCAGAGTCTTCTAATTATTATATTGCAGTAGGGAACTTGAACCCTGAGGATGTTGAGGTAGAGTTGGAATTCAACATAAAGTCCACTCTTTATAACACGACCCAGGCGTATTACAGGTGCTCCATTCATAATCAGTACTGTAGCTTAAAGTTTTCCCTCTTAGGGGCAACTTTTGGTGTCTTAACTTCTCCAGGTCCTGAAGAGGGTATTCAGGAGGATGACTGGTATGTCAAACTGTCATATGGACCACGATGGGTCATCTATTTTCTTGGATCAGGTGTGATGACTGTGCTCCTGTTAATGGCCTTTAGATTTTGTAACATATTCCAAAATGCTGGTGAGAATAGAACGGATTTTCAAGCAGGGGAGGCATCACCTGTACGAACCCCATTGCTTTTACCTAAAGATGATGATGCTTTGAGTTGGGGTTCATCTTATGAATGTATTTCAAATGATGAAGAGGATTTGGAAGAGTTCCTTGGAGTGAGTTCCCTTGAAGGAAAGTCGATAAATGAAGGTAACGACAACAATACTCAGCGTCTTTGTGTCATTTGCTTTGATGGGCCGAGAGACTGCTTCTTTCTTCCATGTGGACACTGTGCTACCTGTTTTACTTGTGGAAAAAGGATTTCTGAAGAGGCTGGAACTTGTCCCATTTGTCGTAGAAAGATGAAGAAAGTGAGGAAGATTTTTTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

375

Amino Acids

42.19

Weight (kDa)

5.28

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APD1-4_N PF16040 44 - 113 7.7e-21 E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M PF16041 138 - 243 3.5e-32 E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3 PF13920 321 - 369 1.1e-13 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 323 - 363 2.9e-06 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AasI GACNNNNNNGTC 1 cut(s) 653
AccB7I CCANNNNNTGG 1 cut(s) 365
AciI CCGC 1 cut(s) 30
AclWI GGATC 6 cut(s) 21, 34, 350, 363, 406, 706
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 2 cut(s) 645, 1080
AfaI GTAC 3 cut(s) 121, 563, 807
AfiI CCNNNNNNNGG 5 cut(s) 365, 588, 589, 629, 727
AgsI TTSAA 8 cut(s) 176, 291, 344, 466, 499, 785, 876, 923
AjiI CACGTC 1 cut(s) 62
AjnI CCWGG 3 cut(s) 336, 528, 616
AjuI GAANNNNNNNTTGG 2 cut(s) 888, 920
AluBI AGCT 2 cut(s) 182, 570
AluI AGCT 2 cut(s) 182, 570
Alw21I GWGCWC 3 cut(s) 213, 548, 720
Alw26I GTCTC 2 cut(s) 68, 990
Alw44I GTGCAC 1 cut(s) 209
AlwI GGATC 6 cut(s) 21, 34, 350, 363, 406, 706
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 46, 729, 989
ApaI GGGCCC 1 cut(s) 50
ApaLI GTGCAC 1 cut(s) 209
ApoI RAATTY 1 cut(s) 494
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AspS9I GGNCC 5 cut(s) 46, 47, 620, 671, 989
AsuC2I CCSGG 2 cut(s) 129, 130
AsuHPI GGTGA 2 cut(s) 776, 792
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 2 cut(s) 620, 671
AxyI CCTNAGG 1 cut(s) 471
BaeGI GKGCMC 2 cut(s) 50, 213
BamHI GGATCC 2 cut(s) 26, 355
BanII GRGCYC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 3 cut(s) 213, 548, 720
BccI CCATC 3 cut(s) 367, 672, 980
BciT130I CCWGG 3 cut(s) 338, 530, 618
BcnI CCSGG 2 cut(s) 129, 130
BcoDI GTCTC 2 cut(s) 68, 990
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 565
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 5 cut(s) 129, 130, 338, 530, 618
Bme18I GGWCC 2 cut(s) 620, 671
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 62
BmgT120I GGNCC 5 cut(s) 46, 47, 620, 671, 989
BmiI GGNNCC 3 cut(s) 28, 48, 357
BmrFI CCNGG 5 cut(s) 129, 130, 338, 530, 618
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 2 cut(s) 806, 829
BmuI ACTGGG 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 600
BpuMI CCSGG 2 cut(s) 129, 130
BsaJI CCNNGG 3 cut(s) 128, 528, 904
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 530, 560
Bsc4I CCNNNNNNNGG 5 cut(s) 365, 588, 589, 629, 727
Bse1I ACTGG 2 cut(s) 48, 653
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 815
BseBI CCWGG 3 cut(s) 338, 530, 618
BseDI CCNNGG 3 cut(s) 128, 528, 904
BseGI GGATG 3 cut(s) 175, 481, 649
BseLI CCNNNNNNNGG 5 cut(s) 365, 588, 589, 629, 727
BseMI GCAATG 1 cut(s) 815
BseMII CTCAG 2 cut(s) 462, 974
BseNI ACTGG 2 cut(s) 48, 653
BseRI GAGGAG 2 cut(s) 150, 153
BseSI GKGCMC 2 cut(s) 50, 213
BshFI GGCC 3 cut(s) 48, 731, 991
BsiHKAI GWGCWC 3 cut(s) 213, 548, 720
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 1062
BslI CCNNNNNNNGG 5 cut(s) 365, 588, 589, 629, 727
BsmAI GTCTC 2 cut(s) 68, 990
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 1062
BsnI GGCC 3 cut(s) 48, 731, 991
BsoBI CYCGRG 1 cut(s) 128
Bsp120I GGGCCC 1 cut(s) 46
Bsp1286I GDGCHC 4 cut(s) 50, 213, 548, 720
Bsp143I GATC 4 cut(s) 26, 355, 411, 698
BspACI CCGC 1 cut(s) 30
BspANI GGCC 3 cut(s) 48, 731, 991
BspCNI CTCAG 2 cut(s) 463, 973
BspLI GGNNCC 3 cut(s) 28, 48, 357
BspPI GGATC 6 cut(s) 21, 34, 350, 363, 406, 706
BsrDI GCAATG 1 cut(s) 815
BsrI ACTGG 2 cut(s) 48, 653
BssECI CCNNGG 3 cut(s) 128, 528, 904
BssMI GATC 4 cut(s) 26, 355, 411, 698
BssT1I CCWWGG 1 cut(s) 904
Bst2UI CCWGG 3 cut(s) 338, 530, 618
Bst4CI ACNGT 4 cut(s) 566, 663, 715, 1025
Bst6I CTCTTC 4 cut(s) 621, 879, 891, 1056
BstDEI CTNAG 3 cut(s) 471, 586, 960
BstF5I GGATG 3 cut(s) 175, 481, 649
BstKTI GATC 4 cut(s) 29, 358, 414, 701
BstMAI GTCTC 2 cut(s) 68, 990
BstMBI GATC 4 cut(s) 26, 355, 411, 698
BstMWI GCNNNNNNNGC 2 cut(s) 54, 794
BstNI CCWGG 3 cut(s) 338, 530, 618
BstSCI CCNGG 5 cut(s) 127, 128, 336, 528, 616
BstSFI CTRYAG 1 cut(s) 565
BstSLI GKGCMC 2 cut(s) 50, 213
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 3 cut(s) 26, 355, 411
BstXI CCANNNNNNTGG 1 cut(s) 761
BstYI RGATCY 3 cut(s) 26, 355, 411
Bsu36I CCTNAGG 1 cut(s) 471
BsuRI GGCC 3 cut(s) 48, 731, 991
BtrI CACGTC 1 cut(s) 62
BtsCI GGATG 3 cut(s) 175, 481, 649
BtsIMutI CAGTG 1 cut(s) 1021
CaiI CAGNNNCTG 1 cut(s) 623
Cfr13I GGNCC 5 cut(s) 46, 47, 620, 671, 989
Cfr9I CCCGGG 1 cut(s) 128
CseI GACGC 1 cut(s) 953
Csp6I GTAC 3 cut(s) 120, 562, 806
CviAII CATG 2 cut(s) 278, 1014
CviJI RGCY 7 cut(s) 48, 182, 336, 570, 731, 991, 1067
CviKI_1 RGCY 7 cut(s) 48, 182, 336, 570, 731, 991, 1067
CviQI GTAC 3 cut(s) 120, 562, 806
DdeI CTNAG 3 cut(s) 471, 586, 960
DpnI GATC 4 cut(s) 28, 357, 413, 700
DpnII GATC 4 cut(s) 26, 355, 411, 698
DrdI GACNNNNNNGTC 1 cut(s) 653
DseDI GACNNNNNNGTC 1 cut(s) 653
Eam1104I CTCTTC 4 cut(s) 621, 879, 891, 1056
EarI CTCTTC 4 cut(s) 621, 879, 891, 1056
EciI GGCGGA 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 904
Eco24I GRGCYC 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 620, 671
Eco57I CTGAAG 2 cut(s) 645, 1080
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 494
EcoRII CCWGG 3 cut(s) 336, 528, 616
EcoT14I CCWWGG 1 cut(s) 904
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 904
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 2 cut(s) 281, 1017
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 1 cut(s) 1062
FatI CATG 2 cut(s) 277, 1013
FauNDI CATATG 2 cut(s) 364, 667
FokI GGATG 3 cut(s) 182, 488, 656
FriOI GRGCYC 1 cut(s) 50
FspBI CTAG 1 cut(s) 258
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 3 cut(s) 48, 731, 991
HapII CCGG 1 cut(s) 129
HgaI GACGC 1 cut(s) 953
Hin1II CATG 2 cut(s) 281, 1017
HinfI GANTC 3 cut(s) 10, 147, 431
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 776, 792
Hpy166II GTNNAC 3 cut(s) 211, 510, 1019
Hpy188I TCNGA 3 cut(s) 93, 430, 1060
Hpy188III TCNNGA 4 cut(s) 167, 258, 623, 638
Hpy8I GTNNAC 3 cut(s) 211, 510, 1019
HpyAV CCTTC 4 cut(s) 297, 317, 917, 935
HpyCH4III ACNGT 4 cut(s) 566, 663, 715, 1025
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 4 cut(s) 211, 320, 374, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 794
HpyF3I CTNAG 3 cut(s) 471, 586, 960
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 2 cut(s) 281, 1017
Kzo9I GATC 4 cut(s) 26, 355, 411, 698
LmnI GCTCC 2 cut(s) 551, 723
LweI GCATC 2 cut(s) 806, 829
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 2 cut(s) 743, 944
MalI GATC 4 cut(s) 28, 357, 413, 700
MboI GATC 4 cut(s) 26, 355, 411, 698
MboII GAAGA 8 cut(s) 426, 638, 896, 908, 1001, 1073, 1111, 1123
MflI RGATCY 3 cut(s) 26, 355, 411
MhlI GDGCHC 4 cut(s) 50, 213, 548, 720
MluCI AATT 8 cut(s) 52, 111, 135, 315, 393, 423, 439, 494
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 439, 471
MseI TTAA 5 cut(s) 99, 305, 572, 608, 725
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 5 cut(s) 129, 130, 338, 530, 618
MvaI CCWGG 3 cut(s) 338, 530, 618
MwoI GCNNNNNNNGC 2 cut(s) 54, 794
NciI CCSGG 2 cut(s) 129, 130
NdeI CATATG 2 cut(s) 364, 667
NdeII GATC 4 cut(s) 26, 355, 411, 698
NlaIII CATG 2 cut(s) 281, 1017
NlaIV GGNNCC 3 cut(s) 28, 48, 357
NmeAIII GCCGAG 1 cut(s) 1017
PcsI WCGNNNNNNNCGW 2 cut(s) 67, 530
PfeI GAWTC 2 cut(s) 10, 147
PflMI CCANNNNNTGG 1 cut(s) 365
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 620
PsiI TTATAA 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 3 cut(s) 336, 528, 616
PspGI CCWGG 3 cut(s) 336, 528, 616
PspN4I GGNNCC 3 cut(s) 28, 48, 357
PspOMI GGGCCC 1 cut(s) 46
PspPI GGNCC 5 cut(s) 46, 47, 620, 671, 989
PspPPI RGGWCCY 1 cut(s) 620
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 3 cut(s) 26, 355, 411
RsaI GTAC 3 cut(s) 121, 563, 807
RsaNI GTAC 3 cut(s) 120, 562, 806
SaqAI TTAA 5 cut(s) 99, 305, 572, 608, 725
Sau3AI GATC 4 cut(s) 26, 355, 411, 698
Sau96I GGNCC 5 cut(s) 46, 47, 620, 671, 989
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 5 cut(s) 129, 130, 338, 530, 618
SduI GDGCHC 4 cut(s) 50, 213, 548, 720
SfaNI GCATC 2 cut(s) 806, 829
SfcI CTRYAG 1 cut(s) 565
SinI GGWCC 2 cut(s) 620, 671
SmaI CCCGGG 1 cut(s) 130
Sse9I AATT 8 cut(s) 52, 111, 135, 315, 393, 423, 439, 494
SsiI CCGC 1 cut(s) 30
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 5 cut(s) 127, 128, 336, 528, 616
StyI CCWWGG 1 cut(s) 904
TaaI ACNGT 4 cut(s) 566, 663, 715, 1025
TaiI ACGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 932
TasI AATT 8 cut(s) 52, 111, 135, 315, 393, 423, 439, 494
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 2 cut(s) 10, 147
Tru1I TTAA 5 cut(s) 99, 305, 572, 608, 725
Tru9I TTAA 5 cut(s) 99, 305, 572, 608, 725
TscAI CASTG 1 cut(s) 1028
TspDTI ATGAA 6 cut(s) 542, 846, 879, 897, 954, 1112
TspGWI ACGGA 2 cut(s) 69, 791
TspMI CCCGGG 1 cut(s) 128
TspRI CASTG 1 cut(s) 1028
Van91I CCANNNNNTGG 1 cut(s) 365
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 620, 671
XapI RAATTY 1 cut(s) 494
XbaI TCTAGA 1 cut(s) 257
XmaI CCCGGG 1 cut(s) 128
XspI CTAG 1 cut(s) 258
ZrmI AGTACT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.