Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001521

Physical Location & Seq

Genomic Coordinates Forward (+)

24164627 .. 24168168

Gene Structure 3542 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1153100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGGAAGACCAACCGTCGTCTTCATCCTCCTCCGCCTCCTCATCTTCTTCTTCTGCTGCTGCTTCGACCAGTACGGACACTTCGGGCGGTGCTGCTGCTTCTGTCGCTACGACGTCGTCTTCGAACGCTTCACAGGTGCGGGAAGAGGAGGACGATGACAATCGGTACCAGCAACACAACCATTTCCGGCATCCGGAGCTGGAGCAAAACCACGAAATGGGCACTTTGTACGGTGGAAATGTGCCCGCGTTAGATGAGGTGTCGACGGTGATTACGGATGACACGTGGTCCTGCATCATCGTCCTCCTCACCTTCTGGTTCTTCGTGTCGTTGACTTTGATTTTGGGAGTTTACGGGTCTTCGAGTTTGGAACTTGGCCCTAATACTTCAATTCTTCTGCAACCTAACCCATTTTTTGTGCAGTCCATAAAGGTACAAGAGCTGAATGCATCAAATCCCGGGCCTGTACTATATGGTTTCTACAGTACTCCACCTCTTGATACTGCCACCATTTGGTCCGAAATGCGTACTGTCTCGGTTCCAGCTGATTCTCATACGGACTGGATATATTACCTAAATGCAGGGTCTCAAATAAGTATATCATACAGCGTGAACTCTCCGAGCTCATCTGTTTTTCTTATTATTGCTCAAGGTCAAGAAGGCCTTGCTCAGTGGCTCGAAGAACCAACATACCCTAATACTACTTTATCGTGGAACCTCATTCATGGTAGTGGTATGATTGAGCAGGACCTATCAAAGTCTTCTACCTACTATGTTTCTGTGGGTAACATGAACGCAGATGTTGTGGAGGTGCAATTAAACTTCACAATGAATGCTTTAGTGTATAATACAACTACGGCTTATTCCAAATGTACTTTCATGGATGAATCCTGTACTTTGAAAATTTTGTTTCCGGAGGGAAATGTGGCTGTGTTGACCACTCCTGGTACAGAGCAGGATACTCCTATGGCAAACCAGTATGTCAAACTATCATATGGGCCAAGATGGACCACATATATCGTTGGTATAGGTGGGATGACTGTGCTCATGTTGTTGGCCTTCAACTTCTTAAACAAGTACCGATTTACCAATGAAGATGGAATAAGAGTACAACATGATGAGTTCAGATCTGAGAGAGCCCCATTGCTTTCCTACAAAGATGATGATCAATCAAGTTGGGGCTCATCTTATGATTCTGCCTCAAACGATGAGGGGGATCTTGAAGACTTTCTGGCTGGAGAAGGGAAGTCGTCAAGAGATGGTGAAAGTAATAACACTCGTCGTCTTTGCGCAATCTGCTTTGATGCTCCCAGGGACTGCTTTTTCCTTCCCTGCGGGCATTGTGTGGCTTGTTTTGAGTGTGCAACAAGGATTGCGGAGGCAGCTGCTACTTGTCCAGTTTGTCGGAGGAACATAAAGAAGGTGAGGAAGATCTTTACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

482

Amino Acids

52.77

Weight (kDa)

4.54

Isoelectric Point (pI)

46.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APD1-4_N	PF16040	154 - 222	2.2e-23	E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M	PF16041	247 - 352	1e-30	E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3	PF13920	427 - 475	7.8e-14	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	429 - 469	2.2e-06	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01450
fragaria_vesca	FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430
malus_domestica	MD03G1070000.v1.1 MD04G1153100.v1.1 MD11G1073800.v1.1 MD12G1166600.v1.1
prunus_persica	Prupe.6G057100_v2.0.a1 Prupe.6G057100_v2.0.a1 Prupe.6G277400_v2.0.a1 Prupe.6G277400_v2.0.a1
pyrus_communis	pycom03g05570 pycom11g06120 pycom12g15850
rosa_chinensis	RchiOBHm_Chr3g0463121 RchiOBHm_Chr5g0069661
rosa_laevigata	RLG00000024811 RLG00000036082
rosa_multiflora	Rmu_sc0000738.1_g000037 Rmu_sc0000766.1_g000040 Rmu_sc0008595.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011420 Rroxscaffold_6G00416950
rosa_rugosa	Rorug03G0061500 Rorug03G0061600 Rorug03G0061700 Rorug05G0399000 Rorug05G0399100
rosa_samantha	Rh3AG119800 Rh3BG124200 Rh3CG126200 Rh3DG125200 Rh5AG455800 Rh5BG474800 Rh5CG498200 Rh5DG486300
rosa_wichuraiana	Rw3G010130 Rw5G042530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	116
Acc16I	TGCGCA	1 cut(s)	1291
Acc65I	GGTACC	1 cut(s)	165
AccB1I	GGYRCC	1 cut(s)	165
AccB7I	CCANNNNNTGG	2 cut(s)	217, 513
AccI	GTMKAC	1 cut(s)	263
AccII	CGCG	1 cut(s)	248
AccIII	TCCGGA	2 cut(s)	193, 913
AciI	CCGC	6 cut(s)	33, 87, 139, 246, 1335, 1376
AclWI	GGATC	1 cut(s)	1224
AcsI	RAATTY	1 cut(s)	903
AcvI	CACGTG	1 cut(s)	285
AcyI	GRCGYC	1 cut(s)	113
AfiI	CCNNNNNNNGG	3 cut(s)	193, 217, 513
AflIII	ACRYGT	1 cut(s)	282
AgsI	TTSAA	4 cut(s)	390, 901, 1063, 1223
AhdI	GACNNNNNGTC	2 cut(s)	13, 286
AjnI	CCWGG	2 cut(s)	943, 1310
AjuI	GAANNNNNNNTTGG	2 cut(s)	860, 892
AluBI	AGCT	5 cut(s)	199, 442, 545, 624, 1385
AluI	AGCT	5 cut(s)	199, 442, 545, 624, 1385
Alw21I	GWGCWC	2 cut(s)	626, 1047
Alw26I	GTCTC	2 cut(s)	538, 591
AlwI	GGATC	1 cut(s)	1224
AlwNI	CAGNNNCTG	1 cut(s)	1317
Ama87I	CYCGRG	1 cut(s)	458
Aor13HI	TCCGGA	2 cut(s)	193, 913
AoxI	GGCC	5 cut(s)	376, 461, 661, 998, 1056
ApeKI	GCWGC	6 cut(s)	56, 59, 92, 95, 1382, 1385
ApoI	RAATTY	1 cut(s)	903
ArsI	GACNNNNNNTTYG	4 cut(s)	103, 135, 325, 357
Asp700I	GAANNNNTTC	1 cut(s)	1227
Asp718I	GGTACC	1 cut(s)	165
AspLEI	GCGC	1 cut(s)	1292
AspS9I	GGNCC	7 cut(s)	288, 377, 461, 516, 748, 998, 1008
AsuC2I	CCSGG	2 cut(s)	459, 460
AsuHPI	GGTGA	4 cut(s)	280, 301, 1274, 1435
AsuII	TTCGAA	1 cut(s)	122
AvaI	CYCGRG	1 cut(s)	458
AvaII	GGWCC	4 cut(s)	288, 516, 748, 1008
BaeGI	GKGCMC	2 cut(s)	224, 246
BanI	GGYRCC	1 cut(s)	165
BanII	GRGCYC	3 cut(s)	626, 1141, 1184
BarI	GAAGNNNNNNTAC	2 cut(s)	64, 96
BbrPI	CACGTG	1 cut(s)	285
BbsI	GAAGAC	6 cut(s)	12, 12, 111, 351, 753, 1230
Bbv12I	GWGCWC	2 cut(s)	626, 1047
BbvI	GCAGC	6 cut(s)	43, 46, 79, 82, 1372, 1394
BccI	CCATC	3 cut(s)	999, 1091, 1253
BceAI	ACGGC	1 cut(s)	873
BciT130I	CCWGG	2 cut(s)	945, 1312
BciVI	GTATCC	1 cut(s)	952
BclI	TGATCA	1 cut(s)	1165
BcnI	CCSGG	2 cut(s)	459, 460
BcoDI	GTCTC	2 cut(s)	538, 591
BfmI	CTRYAG	1 cut(s)	481
BfuI	GTATCC	1 cut(s)	952
BglII	AGATCT	2 cut(s)	1127, 1431
BisI	GCNGC	6 cut(s)	57, 60, 93, 96, 1383, 1386
BlsI	GCNGC	6 cut(s)	58, 61, 94, 97, 1384, 1387
BmcAI	AGTACT	1 cut(s)	487
Bme1390I	CCNGG	4 cut(s)	459, 460, 945, 1312
Bme18I	GGWCC	4 cut(s)	288, 516, 748, 1008
BmeRI	GACNNNNNGTC	2 cut(s)	13, 286
BmeT110I	CYCGRG	1 cut(s)	458
BmgT120I	GGNCC	7 cut(s)	288, 377, 461, 516, 748, 998, 1008
BmiI	GGNNCC	3 cut(s)	167, 540, 716
BmrFI	CCNGG	4 cut(s)	459, 460, 945, 1312
BmsI	GCATC	4 cut(s)	199, 303, 458, 1294
BpiI	GAAGAC	6 cut(s)	12, 12, 111, 351, 753, 1230
BpmI	CTGGAG	2 cut(s)	221, 1257
Bpu14I	TTCGAA	1 cut(s)	122
BpuEI	CTTGAG	1 cut(s)	633
BpuMI	CCSGG	2 cut(s)	459, 460
BsaAI	YACGTR	1 cut(s)	285
BsaBI	GATNNNNATC	2 cut(s)	159, 1164
BsaHI	GRCGYC	1 cut(s)	113
BsaI	GGTCTC	1 cut(s)	591
BsaJI	CCNNGG	3 cut(s)	458, 1310, 1311
BsaWI	WCCGGW	2 cut(s)	193, 913
Bsc4I	CCNNNNNNNGG	3 cut(s)	193, 217, 513
Bse1I	ACTGG	4 cut(s)	69, 566, 976, 1397
Bse3DI	GCAATG	1 cut(s)	1142
Bse8I	GATNNNNATC	2 cut(s)	159, 1164
BseAI	TCCGGA	2 cut(s)	193, 913
BseBI	CCWGG	2 cut(s)	945, 1312
BseDI	CCNNGG	3 cut(s)	458, 1310, 1311
BseGI	GGATG	5 cut(s)	23, 190, 283, 889, 1041
BseJI	GATNNNNATC	2 cut(s)	159, 1164
BseLI	CCNNNNNNNGG	3 cut(s)	193, 217, 513
BseMI	GCAATG	1 cut(s)	1142
BseMII	CTCAG	2 cut(s)	683, 1122
BseNI	ACTGG	4 cut(s)	69, 566, 976, 1397
BseRI	GAGGAG	4 cut(s)	19, 28, 161, 296
BseSI	GKGCMC	2 cut(s)	224, 246
BseXI	GCAGC	6 cut(s)	43, 46, 79, 82, 1372, 1394
BsgI	GTGCAG	1 cut(s)	440
Bsh1236I	CGCG	1 cut(s)	248
BshFI	GGCC	5 cut(s)	378, 463, 663, 1000, 1058
BshNI	GGYRCC	1 cut(s)	165
BsiHKAI	GWGCWC	2 cut(s)	626, 1047
BsiHKCI	CYCGRG	1 cut(s)	458
BsiSI	CCGG	4 cut(s)	187, 194, 459, 914
BslFI	GGGAC	1 cut(s)	1328
BslI	CCNNNNNNNGG	3 cut(s)	193, 217, 513
BsmAI	GTCTC	2 cut(s)	538, 591
BsmFI	GGGAC	1 cut(s)	1328
BsmI	GAATGC	2 cut(s)	451, 838
BsnI	GGCC	5 cut(s)	378, 463, 663, 1000, 1058
Bso31I	GGTCTC	1 cut(s)	591
BsoBI	CYCGRG	1 cut(s)	458
Bsp119I	TTCGAA	1 cut(s)	122
Bsp1286I	GDGCHC	6 cut(s)	224, 246, 626, 1047, 1141, 1184
Bsp13I	TCCGGA	2 cut(s)	193, 913
Bsp143I	GATC	4 cut(s)	1127, 1165, 1216, 1431
BspACI	CCGC	6 cut(s)	33, 87, 139, 246, 1335, 1376
BspANI	GGCC	5 cut(s)	378, 463, 663, 1000, 1058
BspCNI	CTCAG	2 cut(s)	682, 1123
BspEI	TCCGGA	2 cut(s)	193, 913
BspFNI	CGCG	1 cut(s)	248
BspLI	GGNNCC	3 cut(s)	167, 540, 716
BspPI	GGATC	1 cut(s)	1224
BspT104I	TTCGAA	1 cut(s)	122
BspT107I	GGYRCC	1 cut(s)	165
BspTNI	GGTCTC	1 cut(s)	591
BsrDI	GCAATG	1 cut(s)	1142
BsrI	ACTGG	4 cut(s)	69, 566, 976, 1397
BssECI	CCNNGG	3 cut(s)	458, 1310, 1311
BssMI	GATC	4 cut(s)	1127, 1165, 1216, 1431
BssNI	GRCGYC	1 cut(s)	113
Bst2UI	CCWGG	2 cut(s)	945, 1312
Bst4CI	ACNGT	7 cut(s)	15, 233, 268, 485, 532, 1042, 1441
Bst6I	CTCTTC	1 cut(s)	138
BstACI	GRCGYC	1 cut(s)	113
BstBAI	YACGTR	1 cut(s)	285
BstBI	TTCGAA	1 cut(s)	122
BstC8I	GCNNGC	2 cut(s)	246, 1337
BstDEI	CTNAG	2 cut(s)	669, 1131
BstF5I	GGATG	5 cut(s)	23, 190, 283, 889, 1041
BstFNI	CGCG	1 cut(s)	248
BstHHI	GCGC	1 cut(s)	1292
BstKTI	GATC	4 cut(s)	1130, 1168, 1219, 1434
BstMAI	GTCTC	2 cut(s)	538, 591
BstMBI	GATC	4 cut(s)	1127, 1165, 1216, 1431
BstMWI	GCNNNNNNNGC	4 cut(s)	104, 196, 1296, 1382
BstNI	CCWGG	2 cut(s)	945, 1312
BstSCI	CCNGG	4 cut(s)	457, 458, 943, 1310
BstSFI	CTRYAG	1 cut(s)	481
BstSLI	GKGCMC	2 cut(s)	224, 246
BstUI	CGCG	1 cut(s)	248
BstV1I	GCAGC	6 cut(s)	43, 46, 79, 82, 1372, 1394
BstV2I	GAAGAC	6 cut(s)	12, 12, 111, 351, 753, 1230
BstX2I	RGATCY	3 cut(s)	1127, 1216, 1431
BstYI	RGATCY	3 cut(s)	1127, 1216, 1431
BsuI	GTATCC	1 cut(s)	952
BsuRI	GGCC	5 cut(s)	378, 463, 663, 1000, 1058
BtsCI	GGATG	5 cut(s)	23, 190, 283, 889, 1041
BtsIMutI	CAGTG	1 cut(s)	677
Cac8I	GCNNGC	2 cut(s)	246, 1337
CaiI	CAGNNNCTG	1 cut(s)	1317
CfoI	GCGC	1 cut(s)	1292
Cfr13I	GGNCC	7 cut(s)	288, 377, 461, 516, 748, 998, 1008
Cfr9I	CCCGGG	1 cut(s)	458
CviAII	CATG	5 cut(s)	725, 790, 880, 1048, 1115
DdeI	CTNAG	2 cut(s)	669, 1131
DpnI	GATC	4 cut(s)	1129, 1167, 1218, 1433
DpnII	GATC	4 cut(s)	1127, 1165, 1216, 1431
DriI	GACNNNNNGTC	2 cut(s)	13, 286
Eam1104I	CTCTTC	1 cut(s)	138
Eam1105I	GACNNNNNGTC	2 cut(s)	13, 286
EarI	CTCTTC	1 cut(s)	138
EciI	GGCGGA	1 cut(s)	22
Ecl136II	GAGCTC	1 cut(s)	624
Eco147I	AGGCCT	1 cut(s)	663
Eco24I	GRGCYC	3 cut(s)	626, 1141, 1184
Eco31I	GGTCTC	1 cut(s)	591
Eco47I	GGWCC	4 cut(s)	288, 516, 748, 1008
Eco53kI	GAGCTC	1 cut(s)	624
Eco72I	CACGTG	1 cut(s)	285
Eco88I	CYCGRG	1 cut(s)	458
EcoICRI	GAGCTC	1 cut(s)	624
EcoO109I	RGGNCCY	1 cut(s)	748
EcoRII	CCWGG	2 cut(s)	943, 1310
EcoT22I	ATGCAT	1 cut(s)	451
EcoT38I	GRGCYC	3 cut(s)	626, 1141, 1184
FaeI	CATG	5 cut(s)	728, 793, 883, 1051, 1118
FalI	AAGNNNNNCTT	2 cut(s)	648, 680
FaqI	GGGAC	1 cut(s)	1328
FatI	CATG	5 cut(s)	724, 789, 879, 1047, 1114
FauI	CCCGC	3 cut(s)	132, 253, 1328
FauNDI	CATATG	1 cut(s)	994
FbaI	TGATCA	1 cut(s)	1165
FblI	GTMKAC	1 cut(s)	263
Fnu4HI	GCNGC	6 cut(s)	57, 60, 93, 96, 1383, 1386
FokI	GGATG	5 cut(s)	10, 177, 290, 896, 1048
FriOI	GRGCYC	3 cut(s)	626, 1141, 1184
Fsp4HI	GCNGC	6 cut(s)	57, 60, 93, 96, 1383, 1386
FspI	TGCGCA	1 cut(s)	1291
GlaI	GCGC	1 cut(s)	1291
GluI	GCNGC	6 cut(s)	57, 60, 93, 96, 1383, 1386
GsuI	CTGGAG	2 cut(s)	221, 1257
HaeIII	GGCC	5 cut(s)	378, 463, 663, 1000, 1058
HapII	CCGG	4 cut(s)	187, 194, 459, 914
HhaI	GCGC	1 cut(s)	1292
Hin1I	GRCGYC	1 cut(s)	113
Hin1II	CATG	5 cut(s)	728, 793, 883, 1051, 1118
Hin6I	GCGC	1 cut(s)	1290
HinP1I	GCGC	1 cut(s)	1290
HincII	GTYRAC	3 cut(s)	264, 333, 936
HindII	GTYRAC	3 cut(s)	264, 333, 936
HinfI	GANTC	3 cut(s)	548, 887, 1193
HpaII	CCGG	4 cut(s)	187, 194, 459, 914
HphI	GGTGA	4 cut(s)	280, 301, 1274, 1435
Hpy166II	GTNNAC	5 cut(s)	264, 333, 352, 613, 936
Hpy188I	TCNGA	5 cut(s)	520, 621, 1127, 1132, 1407
Hpy188III	TCNNGA	6 cut(s)	194, 497, 656, 914, 1220, 1254
Hpy8I	GTNNAC	5 cut(s)	264, 333, 352, 613, 936
Hpy99I	CGWCG	5 cut(s)	19, 115, 118, 268, 1284
HpyAV	CCTTC	6 cut(s)	322, 653, 1069, 1235, 1337, 1414
HpyCH4III	ACNGT	7 cut(s)	15, 233, 268, 485, 532, 1042, 1441
HpyCH4IV	ACGT	2 cut(s)	113, 284
HpyCH4V	TGCA	7 cut(s)	294, 400, 421, 449, 581, 814, 1364
HpyF10VI	GCNNNNNNNGC	4 cut(s)	104, 196, 1296, 1382
HpyF3I	CTNAG	2 cut(s)	669, 1131
HpySE526I	ACGT	2 cut(s)	113, 284
Hsp92I	GRCGYC	1 cut(s)	113
Hsp92II	CATG	5 cut(s)	728, 793, 883, 1051, 1118
HspAI	GCGC	1 cut(s)	1290
Kpn2I	TCCGGA	2 cut(s)	193, 913
KpnI	GGTACC	1 cut(s)	169
Ksp22I	TGATCA	1 cut(s)	1165
Kzo9I	GATC	4 cut(s)	1127, 1165, 1216, 1431
LmnI	GCTCC	3 cut(s)	196, 202, 1312
Lsp1109I	GCAGC	6 cut(s)	43, 46, 79, 82, 1372, 1394
LweI	GCATC	4 cut(s)	199, 303, 458, 1294
MaeII	ACGT	2 cut(s)	113, 284
MaeIII	GTNAC	1 cut(s)	785
MalI	GATC	4 cut(s)	1129, 1167, 1218, 1433
MboI	GATC	4 cut(s)	1127, 1165, 1216, 1431
MflI	RGATCY	3 cut(s)	1127, 1216, 1431
MhlI	GDGCHC	6 cut(s)	224, 246, 626, 1047, 1141, 1184
MluCI	AATT	3 cut(s)	390, 815, 903
MmeI	TCCRAC	1 cut(s)	1385
Mph1103I	ATGCAT	1 cut(s)	451
MroI	TCCGGA	2 cut(s)	193, 913
MroXI	GAANNNNTTC	1 cut(s)	1227
MseI	TTAA	3 cut(s)	818, 1070, 1444
MslI	CAYNNNNRTG	1 cut(s)	729
MspA1I	CMGCKG	2 cut(s)	545, 1385
MspI	CCGG	4 cut(s)	187, 194, 459, 914
MspR9I	CCNGG	4 cut(s)	459, 460, 945, 1312
Mva1269I	GAATGC	2 cut(s)	451, 838
MvaI	CCWGG	2 cut(s)	945, 1312
MvnI	CGCG	1 cut(s)	248
MwoI	GCNNNNNNNGC	4 cut(s)	104, 196, 1296, 1382
NciI	CCSGG	2 cut(s)	459, 460
NdeI	CATATG	1 cut(s)	994
NdeII	GATC	4 cut(s)	1127, 1165, 1216, 1431
NlaIII	CATG	5 cut(s)	728, 793, 883, 1051, 1118
NlaIV	GGNNCC	3 cut(s)	167, 540, 716
NsbI	TGCGCA	1 cut(s)	1291
NsiI	ATGCAT	1 cut(s)	451
NspV	TTCGAA	1 cut(s)	122
PasI	CCCWGGG	1 cut(s)	1311
PceI	AGGCCT	1 cut(s)	663
PcsI	WCGNNNNNNNCGW	2 cut(s)	119, 281
PctI	GAATGC	2 cut(s)	451, 838
PdmI	GAANNNNTTC	1 cut(s)	1227
PfeI	GAWTC	3 cut(s)	548, 887, 1193
PflFI	GACNNNGTC	1 cut(s)	115
PflMI	CCANNNNNTGG	2 cut(s)	217, 513
PkrI	GCNGC	6 cut(s)	58, 61, 94, 97, 1384, 1387
PmaCI	CACGTG	1 cut(s)	285
PmlI	CACGTG	1 cut(s)	285
Ppu21I	YACGTR	1 cut(s)	285
PpuMI	RGGWCCY	1 cut(s)	748
Psp124BI	GAGCTC	1 cut(s)	626
Psp5II	RGGWCCY	1 cut(s)	748
Psp6I	CCWGG	2 cut(s)	943, 1310
PspCI	CACGTG	1 cut(s)	285
PspGI	CCWGG	2 cut(s)	943, 1310
PspN4I	GGNNCC	3 cut(s)	167, 540, 716
PspPI	GGNCC	7 cut(s)	288, 377, 461, 516, 748, 998, 1008
PspPPI	RGGWCCY	1 cut(s)	748
PstNI	CAGNNNCTG	1 cut(s)	1317
PsuI	RGATCY	3 cut(s)	1127, 1216, 1431
PsyI	GACNNNGTC	1 cut(s)	115
PvuII	CAGCTG	2 cut(s)	545, 1385
RseI	CAYNNNNRTG	1 cut(s)	729
SacI	GAGCTC	1 cut(s)	626
SalI	GTCGAC	1 cut(s)	262
SaqAI	TTAA	3 cut(s)	818, 1070, 1444
SatI	GCNGC	6 cut(s)	57, 60, 93, 96, 1383, 1386
Sau3AI	GATC	4 cut(s)	1127, 1165, 1216, 1431
Sau96I	GGNCC	7 cut(s)	288, 377, 461, 516, 748, 998, 1008
ScaI	AGTACT	1 cut(s)	487
ScrFI	CCNGG	4 cut(s)	459, 460, 945, 1312
SduI	GDGCHC	6 cut(s)	224, 246, 626, 1047, 1141, 1184
SfaNI	GCATC	4 cut(s)	199, 303, 458, 1294
SfcI	CTRYAG	1 cut(s)	481
SfuI	TTCGAA	1 cut(s)	122
SinI	GGWCC	4 cut(s)	288, 516, 748, 1008
SmaI	CCCGGG	1 cut(s)	460
SmiMI	CAYNNNNRTG	1 cut(s)	729
SmlI	CTYRAG	1 cut(s)	648
SmoI	CTYRAG	1 cut(s)	648
Sse9I	AATT	3 cut(s)	390, 815, 903
SseBI	AGGCCT	1 cut(s)	663
SsiI	CCGC	6 cut(s)	33, 87, 139, 246, 1335, 1376
SstI	GAGCTC	1 cut(s)	626
StuI	AGGCCT	1 cut(s)	663
StyD4I	CCNGG	4 cut(s)	457, 458, 943, 1310
TaaI	ACNGT	7 cut(s)	15, 233, 268, 485, 532, 1042, 1441
TaiI	ACGT	2 cut(s)	116, 287
TaqI	TCGA	5 cut(s)	65, 122, 263, 362, 678
TasI	AATT	3 cut(s)	390, 815, 903
TatI	WGTACW	5 cut(s)	466, 485, 872, 893, 1108
TfiI	GAWTC	3 cut(s)	548, 887, 1193
Tru1I	TTAA	3 cut(s)	818, 1070, 1444
Tru9I	TTAA	3 cut(s)	818, 1070, 1444
TscAI	CASTG	1 cut(s)	677
TseI	GCWGC	6 cut(s)	56, 59, 92, 95, 1382, 1385
TspDTI	ATGAA	7 cut(s)	12, 713, 806, 845, 868, 900, 1107
TspGWI	ACGGA	3 cut(s)	89, 290, 572
TspMI	CCCGGG	1 cut(s)	458
TspRI	CASTG	1 cut(s)	677
Tth111I	GACNNNGTC	1 cut(s)	115
Van91I	CCANNNNNTGG	2 cut(s)	217, 513
VpaK11BI	GGWCC	4 cut(s)	288, 516, 748, 1008
XapI	RAATTY	1 cut(s)	903
XmaI	CCCGGG	1 cut(s)	458
XmiI	GTMKAC	1 cut(s)	263
XmnI	GAANNNNTTC	1 cut(s)	1227
ZraI	GACGTC	1 cut(s)	114
ZrmI	AGTACT	1 cut(s)	487
Zsp2I	ATGCAT	1 cut(s)	451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.