MD03G1108200.v1.1

L-ascorbate peroxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002887
Physical Location & Seq
Forward (+)
9396035 .. 9398958
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1108200.v1.1.491

Sequence Viewer

Length: 648 bp
ATGCTTCGCATCGCATGGCATTCGGCGGGAACTTACGACACCAAGACGAAGACCGGGGGGCCCTTCGGAACTATGAGGTGCCCGGCTGAGCAAGCTCACGGGGCCAACAATGGTCTCGAGATCGCTGTCAGGCTCTTGGAGCCCATCAAGCAACAGTTCCCCATCCTCTCCTACGCCGACTTCTACCAGTTGGCTGGTGTTGTTGCTGTTGAGATTACTGGTGGGCCTGATGTCCCATTCCACCCAGGAAGGACGGATGCCCCCGTGCCACCACCAGAGGGCCGTCTTCCTGATGCTACCAAGGGTAATGACCATTTGAGGGATGTCTTCTGCAAGACCATGGGCCTCAGCGACAAGGATATTGTTACTCTCTCCGGTGGTCACACCCTGGGAAGGTGCCACAAGGAGCGATCTGGATTTGAAGGACCTTGGACTCCCAACCCCCTTATCTTTGACAACTCCTACTTCAAGGTGCTTCTTGGTGGAGACCAGGAAGGTCTTCTAATGCTTCCAACTGACAAGGCTCTTCTGGATGACCCTGTCTTCCGCCCTCTTGTGGAAAAATACGCTGCGGATGAAGATGCTTTCTTTGCTGACTATGCTGAATCTCACATGAAGCTCTCCGAGCTTGGGTTTGCTGAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00480 map00053 map00480
Pfam Domains
Protein Families
peroxidase

Protein Analysis

216

Amino Acids

23.46

Weight (kDa)

5.1

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 1 - 192 5.9e-41 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 78, 396
AciI CCGC 3 cut(s) 26, 547, 572
AfiI CCNNNNNNNGG 5 cut(s) 278, 319, 393, 556, 630
AgsI TTSAA 2 cut(s) 422, 469
AjnI CCWGG 3 cut(s) 244, 387, 489
AluBI AGCT 3 cut(s) 95, 619, 628
AluI AGCT 3 cut(s) 95, 619, 628
Alw26I GTCTC 2 cut(s) 119, 480
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 6 cut(s) 59, 102, 224, 280, 343, 642
ApaI GGGCCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 7 cut(s) 59, 60, 102, 224, 280, 343, 425
AsuC2I CCSGG 2 cut(s) 55, 83
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 2 cut(s) 63, 83
BanI GGYRCC 2 cut(s) 78, 396
BanII GRGCYC 2 cut(s) 63, 144
BbsI GAAGAC 5 cut(s) 56, 278, 319, 491, 535
BbvCI CCTCAGC 2 cut(s) 347, 639
BbvI GCAGC 1 cut(s) 556
BccI CCATC 2 cut(s) 152, 170
BceAI ACGGC 1 cut(s) 267
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 3 cut(s) 246, 389, 491
BcnI CCSGG 2 cut(s) 55, 83
BcoDI GTCTC 2 cut(s) 119, 480
BisI GCNGC 1 cut(s) 570
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 5 cut(s) 55, 83, 246, 389, 491
Bme18I GGWCC 1 cut(s) 425
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 7 cut(s) 59, 60, 102, 224, 280, 343, 425
BmiI GGNNCC 6 cut(s) 60, 61, 80, 103, 141, 398
BmrFI CCNGG 5 cut(s) 55, 83, 246, 389, 491
BmsI GCATC 4 cut(s) 18, 247, 283, 571
BpiI GAAGAC 5 cut(s) 56, 278, 319, 491, 535
Bpu10I CCTNAGC 2 cut(s) 347, 639
Bpu1102I GCTNAGC 1 cut(s) 87
BpuMI CCSGG 2 cut(s) 55, 83
BsaI GGTCTC 2 cut(s) 119, 480
BsaJI CCNNGG 7 cut(s) 54, 244, 300, 339, 387, 388, 428
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 5 cut(s) 278, 319, 393, 556, 630
Bse1I ACTGG 2 cut(s) 187, 223
BseBI CCWGG 3 cut(s) 246, 389, 491
BseDI CCNNGG 7 cut(s) 54, 244, 300, 339, 387, 388, 428
BseGI GGATG 5 cut(s) 162, 262, 328, 538, 580
BseLI CCNNNNNNNGG 5 cut(s) 278, 319, 393, 556, 630
BseMII CTCAG 3 cut(s) 78, 361, 630
BseNI ACTGG 2 cut(s) 187, 223
BseSI GKGCMC 2 cut(s) 63, 83
BseXI GCAGC 1 cut(s) 556
BshFI GGCC 6 cut(s) 61, 104, 226, 282, 345, 644
BshNI GGYRCC 2 cut(s) 78, 396
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 54, 83, 375
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 5 cut(s) 278, 319, 393, 556, 630
BsmAI GTCTC 2 cut(s) 119, 480
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 6 cut(s) 61, 104, 226, 282, 345, 644
Bso31I GGTCTC 2 cut(s) 119, 480
BsoBI CYCGRG 1 cut(s) 116
Bsp120I GGGCCC 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 63, 83, 144
Bsp143I GATC 2 cut(s) 120, 410
Bsp1720I GCTNAGC 1 cut(s) 87
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 26, 547, 572
BspANI GGCC 6 cut(s) 61, 104, 226, 282, 345, 644
BspCNI CTCAG 3 cut(s) 79, 360, 631
BspLI GGNNCC 6 cut(s) 60, 61, 80, 103, 141, 398
BspQI GCTCTTC 1 cut(s) 531
BspT107I GGYRCC 2 cut(s) 78, 396
BspTNI GGTCTC 2 cut(s) 119, 480
BsrI ACTGG 2 cut(s) 187, 223
BssECI CCNNGG 7 cut(s) 54, 244, 300, 339, 387, 388, 428
BssMI GATC 2 cut(s) 120, 410
BssT1I CCWWGG 3 cut(s) 300, 339, 428
Bst2UI CCWGG 3 cut(s) 246, 389, 491
Bst4CI ACNGT 1 cut(s) 156
Bst6I CTCTTC 1 cut(s) 531
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 87, 347, 639
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 5 cut(s) 162, 262, 328, 538, 580
BstKTI GATC 2 cut(s) 123, 413
BstMAI GTCTC 2 cut(s) 119, 480
BstMBI GATC 2 cut(s) 120, 410
BstMWI GCNNNNNNNGC 7 cut(s) 92, 101, 139, 148, 590, 599, 625
BstNI CCWGG 3 cut(s) 246, 389, 491
BstSCI CCNGG 5 cut(s) 53, 81, 244, 387, 489
BstSLI GKGCMC 2 cut(s) 63, 83
BstV1I GCAGC 1 cut(s) 556
BstV2I GAAGAC 5 cut(s) 56, 278, 319, 491, 535
BstXI CCANNNNNNTGG 1 cut(s) 194
BsuRI GGCC 6 cut(s) 61, 104, 226, 282, 345, 644
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 5 cut(s) 162, 262, 328, 538, 580
Cac8I GCNNGC 1 cut(s) 93
Cfr13I GGNCC 7 cut(s) 59, 60, 102, 224, 280, 343, 425
CviAII CATG 3 cut(s) 15, 340, 613
DdeI CTNAG 3 cut(s) 87, 347, 639
DpnI GATC 2 cut(s) 122, 412
DpnII GATC 2 cut(s) 120, 410
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
EciI GGCGGA 1 cut(s) 536
Eco130I CCWWGG 3 cut(s) 300, 339, 428
Eco147I AGGCCT 1 cut(s) 644
Eco24I GRGCYC 2 cut(s) 63, 144
Eco31I GGTCTC 2 cut(s) 119, 480
Eco47I GGWCC 1 cut(s) 425
Eco88I CYCGRG 1 cut(s) 116
EcoO109I RGGNCCY 3 cut(s) 59, 60, 425
EcoRII CCWGG 3 cut(s) 244, 387, 489
EcoT14I CCWWGG 3 cut(s) 300, 339, 428
EcoT38I GRGCYC 2 cut(s) 63, 144
ErhI CCWWGG 3 cut(s) 300, 339, 428
FaeI CATG 3 cut(s) 18, 343, 616
FaiI YATR 5 cut(s) 16, 74, 341, 600, 614
FaqI GGGAC 1 cut(s) 218
FatI CATG 3 cut(s) 14, 339, 612
FauI CCCGC 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 570
FokI GGATG 5 cut(s) 149, 269, 335, 545, 587
FriOI GRGCYC 2 cut(s) 63, 144
Fsp4HI GCNGC 1 cut(s) 570
GluI GCNGC 1 cut(s) 570
HaeIII GGCC 6 cut(s) 61, 104, 226, 282, 345, 644
HapII CCGG 3 cut(s) 54, 83, 375
Hin1II CATG 3 cut(s) 18, 343, 616
HinfI GANTC 2 cut(s) 433, 605
HpaII CCGG 3 cut(s) 54, 83, 375
Hpy188I TCNGA 2 cut(s) 68, 625
Hpy188III TCNNGA 5 cut(s) 116, 118, 290, 414, 530
HpyAV CCTTC 5 cut(s) 73, 243, 387, 416, 488
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 101, 139, 148, 590, 599, 625
HpyF3I CTNAG 3 cut(s) 87, 347, 639
Hsp92II CATG 3 cut(s) 18, 343, 616
Kzo9I GATC 2 cut(s) 120, 410
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 2 cut(s) 139, 406
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 4 cut(s) 18, 247, 283, 571
MaeIII GTNAC 2 cut(s) 364, 380
MalI GATC 2 cut(s) 122, 412
MboI GATC 2 cut(s) 120, 410
MboII GAAGA 7 cut(s) 61, 278, 319, 491, 518, 535, 590
MhlI GDGCHC 3 cut(s) 63, 83, 144
MlyI GAGTC 1 cut(s) 427
MmeI TCCRAC 1 cut(s) 536
MnlI CCTC 7 cut(s) 69, 176, 271, 312, 356, 561, 634
MroXI GAANNNNTTC 1 cut(s) 498
MspI CCGG 3 cut(s) 54, 83, 375
MspR9I CCNGG 5 cut(s) 55, 83, 246, 389, 491
Mva1269I GAATGC 1 cut(s) 19
MvaI CCWGG 3 cut(s) 246, 389, 491
MwoI GCNNNNNNNGC 7 cut(s) 92, 101, 139, 148, 590, 599, 625
NciI CCSGG 2 cut(s) 55, 83
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 120, 410
NlaIII CATG 3 cut(s) 18, 343, 616
NlaIV GGNNCC 6 cut(s) 60, 61, 80, 103, 141, 398
NmuCI GTSAC 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 116
PasI CCCWGGG 1 cut(s) 388
PceI AGGCCT 1 cut(s) 644
PciSI GCTCTTC 1 cut(s) 531
PctI GAATGC 1 cut(s) 19
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 605
PflFI GACNNNGTC 1 cut(s) 539
PkrI GCNGC 1 cut(s) 571
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PpuMI RGGWCCY 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 3 cut(s) 244, 387, 489
PspGI CCWGG 3 cut(s) 244, 387, 489
PspN4I GGNNCC 6 cut(s) 60, 61, 80, 103, 141, 398
PspOMI GGGCCC 1 cut(s) 59
PspPI GGNCC 7 cut(s) 59, 60, 102, 224, 280, 343, 425
PspPPI RGGWCCY 1 cut(s) 425
PsyI GACNNNGTC 1 cut(s) 539
SapI GCTCTTC 1 cut(s) 531
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 2 cut(s) 120, 410
Sau96I GGNCC 7 cut(s) 59, 60, 102, 224, 280, 343, 425
SchI GAGTC 1 cut(s) 427
ScrFI CCNGG 5 cut(s) 55, 83, 246, 389, 491
SduI GDGCHC 3 cut(s) 63, 83, 144
SetI ASST 8 cut(s) 80, 97, 398, 430, 474, 499, 621, 630
SfaNI GCATC 4 cut(s) 18, 247, 283, 571
Sfr274I CTCGAG 1 cut(s) 116
SinI GGWCC 1 cut(s) 425
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SseBI AGGCCT 1 cut(s) 644
SsiI CCGC 3 cut(s) 26, 547, 572
StuI AGGCCT 1 cut(s) 644
StyD4I CCNGG 5 cut(s) 53, 81, 244, 387, 489
StyI CCWWGG 3 cut(s) 300, 339, 428
TaaI ACNGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 117
TfiI GAWTC 1 cut(s) 605
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 2 cut(s) 591, 629
TspGWI ACGGA 1 cut(s) 269
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.