Prupe.6G091600_v2.0.a1

L-ascorbate peroxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0002887
Physical Location & Seq
Forward (+)
6288878 .. 6291737
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G091600.2

Sequence Viewer

Length: 753 bp
ATGGGGAAGAGCTACCCTACCGTGAGCGAAGAGTACAAGAAGGCCATCGACAAGGCCAAGAGGAAGCTCAGAGGCTTCATCGCCGAGAAGGCCTGCGCTCCACTCATGCTTCGTATCGCATGGCATTCTGCTGGTACCTACGATTCGAGAACAAAAACCGGAGGCCCATTCGGAACTATGAAGCACGCAGCTGAGCAATCGCACGGGGCCAACGCTGGTCTCGATATCGCTGTCAGGCTCTTGGAGCCCATCAAGCAACAGTTCCCAATTCTCTCTTACGCTGACTTCTATCAGTTGGCTGGTGTTGTTGCTGTTGAGATTACTGGTGGGCCTGATGTCCCCTTCCATCCAGGAAGAGAGGACAAGCCTGAGCCACCTCCAGAAGGCCGTCTTCCTGATGCTACCAAGGGTAATGACCATTTGAGGGATGTCTTTGGCAAAACCATGGGCCTCAGCGACCAGGATATTGTTGCTCTCTCTGGCGGTCACACTCTGGGAAGGTGCCACAAGGAGCGATCTGGATTTGAAGGACCCTGGACTCCCAACCCCCTCATCTTCGACAACTCGTATTTCACGGTGCTCCTGAGTGAAAAGTATGATGGTCTTCTAATGCTTCCAACTGACACTGCCCTTCTGTCTGACCCTGTCTTCCGCCCTCTTGTTGAAAAATATGCTGCGGATGAAGATGCCTTCTTTGCTGATTACGCTGCAGCACACCAGAGGCTTTCTGAGCTTGGGTTTGCTGAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00480 map00053 map00480
Pfam Domains
Protein Families
peroxidase

Protein Analysis

251

Amino Acids

27.37

Weight (kDa)

5.76

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 134
AccB1I GGYRCC 2 cut(s) 134, 501
AciI CCGC 3 cut(s) 483, 652, 677
AfaI GTAC 2 cut(s) 35, 136
AfiI CCNNNNNNNGG 2 cut(s) 383, 424
AgsI TTSAA 2 cut(s) 527, 665
AjnI CCWGG 3 cut(s) 349, 459, 533
AluBI AGCT 4 cut(s) 12, 67, 191, 733
AluI AGCT 4 cut(s) 12, 67, 191, 733
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 224
AoxI GGCC 9 cut(s) 42, 54, 90, 163, 207, 329, 385, 448, 747
ApeKI GCWGC 4 cut(s) 188, 674, 707, 710
Asp718I GGTACC 1 cut(s) 134
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 5 cut(s) 164, 207, 329, 448, 530
AvaII GGWCC 1 cut(s) 530
BanI GGYRCC 2 cut(s) 134, 501
BanII GRGCYC 1 cut(s) 249
BbsI GAAGAC 3 cut(s) 383, 596, 640
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 2 cut(s) 452, 744
BbvI GCAGC 4 cut(s) 200, 661, 694, 722
BccI CCATC 4 cut(s) 53, 257, 354, 593
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 3 cut(s) 351, 461, 535
BcoDI GTCTC 1 cut(s) 224
BfmI CTRYAG 1 cut(s) 708
BglI GCCNNNNNGGC 1 cut(s) 89
BisI GCNGC 4 cut(s) 189, 675, 708, 711
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 4 cut(s) 190, 676, 709, 712
Bme1390I CCNGG 3 cut(s) 351, 461, 535
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 5 cut(s) 164, 207, 329, 448, 530
BmiI GGNNCC 5 cut(s) 136, 208, 246, 503, 532
BmrFI CCNGG 3 cut(s) 351, 461, 535
BmsI GCATC 2 cut(s) 388, 676
BpiI GAAGAC 3 cut(s) 383, 596, 640
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 3 cut(s) 369, 452, 744
Bpu1102I GCTNAGC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 405, 444, 533
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 424
Bse1I ACTGG 1 cut(s) 328
BseBI CCWGG 3 cut(s) 351, 461, 535
BseDI CCNNGG 3 cut(s) 405, 444, 533
BseGI GGATG 3 cut(s) 346, 433, 685
BseLI CCNNNNNNNGG 2 cut(s) 383, 424
BseMII CTCAG 7 cut(s) 82, 183, 360, 466, 575, 720, 735
BseNI ACTGG 1 cut(s) 328
BseXI GCAGC 4 cut(s) 200, 661, 694, 722
BshFI GGCC 9 cut(s) 44, 56, 92, 165, 209, 331, 387, 450, 749
BshNI GGYRCC 2 cut(s) 134, 501
BsiHKAI GWGCWC 1 cut(s) 582
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 323
BslI CCNNNNNNNGG 2 cut(s) 383, 424
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 9 cut(s) 44, 56, 92, 165, 209, 331, 387, 450, 749
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 249, 582
Bsp143I GATC 1 cut(s) 515
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 3 cut(s) 483, 652, 677
BspANI GGCC 9 cut(s) 44, 56, 92, 165, 209, 331, 387, 450, 749
BspCNI CTCAG 7 cut(s) 81, 184, 361, 465, 576, 721, 736
BspLI GGNNCC 5 cut(s) 136, 208, 246, 503, 532
BspMAI CTGCAG 1 cut(s) 712
BspQI GCTCTTC 1 cut(s) 2
BspT107I GGYRCC 2 cut(s) 134, 501
BspTNI GGTCTC 1 cut(s) 224
BsrI ACTGG 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 405, 444, 533
BssMI GATC 1 cut(s) 515
BssT1I CCWWGG 2 cut(s) 405, 444
Bst2UI CCWGG 3 cut(s) 351, 461, 535
Bst4CI ACNGT 3 cut(s) 22, 261, 577
Bst6I CTCTTC 3 cut(s) 2, 24, 349
BstC8I GCNNGC 2 cut(s) 94, 186
BstDEI CTNAG 7 cut(s) 68, 192, 369, 452, 584, 729, 744
BstDSI CCRYGG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 3 cut(s) 346, 433, 685
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 1 cut(s) 518
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 1 cut(s) 515
BstMWI GCNNNNNNNGC 6 cut(s) 89, 244, 253, 695, 704, 730
BstNI CCWGG 3 cut(s) 351, 461, 535
BstSCI CCNGG 3 cut(s) 349, 459, 533
BstSFI CTRYAG 1 cut(s) 708
BstV1I GCAGC 4 cut(s) 200, 661, 694, 722
BstV2I GAAGAC 3 cut(s) 383, 596, 640
BsuRI GGCC 9 cut(s) 44, 56, 92, 165, 209, 331, 387, 450, 749
BtgI CCRYGG 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 3 cut(s) 346, 433, 685
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 1 cut(s) 624
Cac8I GCNNGC 2 cut(s) 94, 186
CfoI GCGC 1 cut(s) 98
Cfr13I GGNCC 5 cut(s) 164, 207, 329, 448, 530
Csp6I GTAC 2 cut(s) 34, 135
CviAII CATG 3 cut(s) 106, 120, 445
CviQI GTAC 2 cut(s) 34, 135
DdeI CTNAG 7 cut(s) 68, 192, 369, 452, 584, 729, 744
DpnI GATC 1 cut(s) 517
DpnII GATC 1 cut(s) 515
Eam1104I CTCTTC 3 cut(s) 2, 24, 349
EarI CTCTTC 3 cut(s) 2, 24, 349
EciI GGCGGA 1 cut(s) 641
Eco130I CCWWGG 2 cut(s) 405, 444
Eco147I AGGCCT 2 cut(s) 92, 749
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 224
Eco32I GATATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 530
EcoRII CCWGG 3 cut(s) 349, 459, 533
EcoRV GATATC 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 405, 444
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 2 cut(s) 405, 444
FaeI CATG 3 cut(s) 109, 123, 448
FaiI YATR 6 cut(s) 107, 121, 179, 446, 597, 672
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 105, 119, 444
Fnu4HI GCNGC 4 cut(s) 189, 675, 708, 711
FokI GGATG 3 cut(s) 333, 440, 692
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 189, 675, 708, 711
GlaI GCGC 1 cut(s) 97
GluI GCNGC 4 cut(s) 189, 675, 708, 711
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 9 cut(s) 44, 56, 92, 165, 209, 331, 387, 450, 749
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 98
Hin1II CATG 3 cut(s) 109, 123, 448
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HinfI GANTC 2 cut(s) 143, 538
HpaII CCGG 1 cut(s) 159
Hpy188I TCNGA 4 cut(s) 71, 173, 640, 730
Hpy188III TCNNGA 6 cut(s) 147, 221, 380, 395, 519, 583
HpyAV CCTTC 8 cut(s) 34, 82, 352, 377, 492, 521, 641, 700
HpyCH4III ACNGT 3 cut(s) 22, 261, 577
HpyCH4V TGCA 1 cut(s) 710
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 244, 253, 695, 704, 730
HpyF3I CTNAG 7 cut(s) 68, 192, 369, 452, 584, 729, 744
Hsp92II CATG 3 cut(s) 109, 123, 448
HspAI GCGC 1 cut(s) 96
KpnI GGTACC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 515
LguI GCTCTTC 1 cut(s) 2
LmnI GCTCC 4 cut(s) 103, 244, 511, 585
Lsp1109I GCAGC 4 cut(s) 200, 661, 694, 722
LweI GCATC 2 cut(s) 388, 676
MaeIII GTNAC 1 cut(s) 485
MalI GATC 1 cut(s) 517
MboI GATC 1 cut(s) 515
MboII GAAGA 8 cut(s) 19, 41, 366, 383, 547, 596, 640, 695
MhlI GDGCHC 2 cut(s) 249, 582
MluCI AATT 1 cut(s) 267
MlyI GAGTC 1 cut(s) 532
MmeI TCCRAC 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 3 cut(s) 351, 461, 535
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 3 cut(s) 351, 461, 535
MwoI GCNNNNNNNGC 6 cut(s) 89, 244, 253, 695, 704, 730
NcoI CCATGG 1 cut(s) 444
NdeII GATC 1 cut(s) 515
NlaIII CATG 3 cut(s) 109, 123, 448
NlaIV GGNNCC 5 cut(s) 136, 208, 246, 503, 532
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 485
PceI AGGCCT 2 cut(s) 92, 749
PciSI GCTCTTC 1 cut(s) 2
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 143
PflFI GACNNNGTC 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 4 cut(s) 190, 676, 709, 712
PleI GAGTC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 532
PpuMI RGGWCCY 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 530
Psp6I CCWGG 3 cut(s) 349, 459, 533
PspGI CCWGG 3 cut(s) 349, 459, 533
PspN4I GGNNCC 5 cut(s) 136, 208, 246, 503, 532
PspPI GGNCC 5 cut(s) 164, 207, 329, 448, 530
PspPPI RGGWCCY 1 cut(s) 530
PstI CTGCAG 1 cut(s) 712
PsyI GACNNNGTC 1 cut(s) 644
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 35, 136
RsaNI GTAC 2 cut(s) 34, 135
SapI GCTCTTC 1 cut(s) 2
SatI GCNGC 4 cut(s) 189, 675, 708, 711
Sau3AI GATC 1 cut(s) 515
Sau96I GGNCC 5 cut(s) 164, 207, 329, 448, 530
SchI GAGTC 1 cut(s) 532
ScrFI CCNGG 3 cut(s) 351, 461, 535
SduI GDGCHC 2 cut(s) 249, 582
SetI ASST 7 cut(s) 14, 69, 140, 193, 379, 503, 735
SfaNI GCATC 2 cut(s) 388, 676
SfcI CTRYAG 1 cut(s) 708
SinI GGWCC 1 cut(s) 530
Sse9I AATT 1 cut(s) 267
SseBI AGGCCT 2 cut(s) 92, 749
SsiI CCGC 3 cut(s) 483, 652, 677
StuI AGGCCT 2 cut(s) 92, 749
StyD4I CCNGG 3 cut(s) 349, 459, 533
StyI CCWWGG 2 cut(s) 405, 444
TaaI ACNGT 3 cut(s) 22, 261, 577
TaqI TCGA 4 cut(s) 48, 146, 222, 558
TasI AATT 1 cut(s) 267
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 1 cut(s) 143
TscAI CASTG 1 cut(s) 631
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 4 cut(s) 188, 674, 707, 710
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 3 cut(s) 67, 194, 696
TspRI CASTG 1 cut(s) 631
Tth111I GACNNNGTC 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 530
XagI CCTNNNNNAGG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.