MD04G1194600.v1.1

Universal stress protein A-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002806
Physical Location & Seq
Reverse (-)
28376752 .. 28378412
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1194600.v1.1.491

Sequence Viewer

Length: 504 bp
ATGACTGACGTGGCAGAAAGCGAGCGGAGGATCCTCGTGGCCGTCGACGAAGGCGAGGAGAGCATGCATGCGCTGTCGTGGTGCCTGAAGAACGTTGCAGGGCAAAATTCCAAAGATACCCTTGTCCTCCTCCACGCCAAGCCCCCGCGAGCTGTGTTCACGGCTCTAGATGGCACAGCGTATTTGTTTTCGTCTGATATTTTGGCGGCAACGGAGAAGTACGGCGCTGATGTCGCGGACTGCGTTATGGAGAAGGCGAGGAAGATGTGCAAGGACCTCCAGCCTGATCTTAAGGCGGAGACGAAAGTAGAGAGCGGAGATCCGAGGGATGTCATTTGCCAGATGGTAGAGCGGGTTCGTGCTGACGTGTTGGTTATGGGAAGCCATGGCTATGGCGTCATCAAGAGGGCTTTTCTTGGGAGTGTGAGCAATCACTGTGCACAGAATGTCAAGTGTCCTGTCTTGATCGTGAAGAAGCCAAAAACAAATGCCGGGAGCAAATGA

Protein Analysis

168

Amino Acids

18.15

Weight (kDa)

7.6

Isoelectric Point (pI)

25.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 9 - 158 1.1e-29 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 81
AccBSI CCGCTC 3 cut(s) 25, 315, 352
AccI GTMKAC 1 cut(s) 45
AccII CGCG 2 cut(s) 148, 236
AciI CCGC 7 cut(s) 25, 146, 206, 236, 296, 315, 352
AclI AACGTT 1 cut(s) 93
AclWI GGATC 3 cut(s) 25, 38, 314
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 1 cut(s) 221
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 1 cut(s) 366
AjiI CACGTC 2 cut(s) 10, 367
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 1 cut(s) 293
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 3 cut(s) 25, 38, 314
AoxI GGCC 1 cut(s) 39
ApaLI GTGCAC 1 cut(s) 438
ApoI RAATTY 1 cut(s) 106
AspLEI GCGC 2 cut(s) 73, 227
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 493
AvaII GGWCC 1 cut(s) 274
BaeGI GKGCMC 1 cut(s) 442
BaeI ACNNNNGTAYC 2 cut(s) 108, 141
BamHI GGATCC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 81
BauI CACGAG 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 442
BccI CCATC 2 cut(s) 164, 337
BceAI ACGGC 3 cut(s) 26, 177, 238
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 228
BfrI CTTAAG 1 cut(s) 290
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 493
Bme18I GGWCC 1 cut(s) 274
BmgBI CACGTC 2 cut(s) 10, 367
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 2 cut(s) 32, 83
BmrFI CCNGG 1 cut(s) 493
BpmI CTGGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 493
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 323, 385
BseDI CCNNGG 2 cut(s) 323, 385
BseGI GGATG 1 cut(s) 334
BseRI GAGGAG 2 cut(s) 71, 119
BseSI GKGCMC 1 cut(s) 442
Bsh1236I CGCG 2 cut(s) 148, 236
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 442
BsiSI CCGG 1 cut(s) 492
BsmAI GTCTC 1 cut(s) 293
BsmBI CGTCTC 1 cut(s) 293
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 4 cut(s) 30, 286, 319, 465
Bsp19I CCATGG 1 cut(s) 385
BspACI CCGC 7 cut(s) 25, 146, 206, 236, 296, 315, 352
BspANI GGCC 1 cut(s) 41
BspFNI CGCG 2 cut(s) 148, 236
BspLI GGNNCC 2 cut(s) 32, 83
BspPI GGATC 3 cut(s) 25, 38, 314
BspT107I GGYRCC 1 cut(s) 81
BspTI CTTAAG 1 cut(s) 290
BsrBI CCGCTC 3 cut(s) 25, 315, 352
BssECI CCNNGG 2 cut(s) 323, 385
BssMI GATC 4 cut(s) 30, 286, 319, 465
BssNI GRCGYC 1 cut(s) 396
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 385
Bst2BI CACGAG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 437
BstACI GRCGYC 1 cut(s) 396
BstAFI CTTAAG 1 cut(s) 290
BstC8I GCNNGC 4 cut(s) 23, 65, 69, 150
BstDSI CCRYGG 1 cut(s) 385
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 2 cut(s) 148, 236
BstH2I RGCGCY 1 cut(s) 228
BstHHI GCGC 2 cut(s) 73, 227
BstKTI GATC 4 cut(s) 33, 289, 322, 468
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 4 cut(s) 30, 286, 319, 465
BstMWI GCNNNNNNNGC 2 cut(s) 60, 233
BstNSI RCATGY 2 cut(s) 67, 71
BstSCI CCNGG 1 cut(s) 491
BstSLI GKGCMC 1 cut(s) 442
BstUI CGCG 2 cut(s) 148, 236
BstX2I RGATCY 2 cut(s) 30, 319
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 2 cut(s) 30, 319
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 385
BtrI CACGTC 2 cut(s) 10, 367
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 433
Cac8I GCNNGC 4 cut(s) 23, 65, 69, 150
CfoI GCGC 2 cut(s) 73, 227
Cfr13I GGNCC 1 cut(s) 274
CseI GACGC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 220
CviAII CATG 3 cut(s) 64, 68, 386
CviJI RGCY 9 cut(s) 41, 142, 152, 164, 283, 384, 390, 410, 478
CviKI_1 RGCY 9 cut(s) 41, 142, 152, 164, 283, 384, 390, 410, 478
CviQI GTAC 1 cut(s) 220
DpnI GATC 4 cut(s) 32, 288, 321, 467
DpnII GATC 4 cut(s) 30, 286, 319, 465
EaeI YGGCCR 1 cut(s) 39
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 385
EcoT22I ATGCAT 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 3 cut(s) 67, 71, 389
FaiI YATR 6 cut(s) 65, 69, 248, 377, 387, 393
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FatI CATG 3 cut(s) 63, 67, 385
FauI CCCGC 2 cut(s) 153, 345
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 1 cut(s) 167
GlaI GCGC 2 cut(s) 72, 226
GluI GCNGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 228
HaeIII GGCC 1 cut(s) 41
HapII CCGG 1 cut(s) 492
HgaI GACGC 1 cut(s) 385
HhaI GCGC 2 cut(s) 73, 227
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 3 cut(s) 67, 71, 389
Hin6I GCGC 2 cut(s) 71, 225
HinP1I GCGC 2 cut(s) 71, 225
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HpaII CCGG 1 cut(s) 492
Hpy166II GTNNAC 3 cut(s) 46, 159, 440
Hpy188I TCNGA 2 cut(s) 196, 324
Hpy188III TCNNGA 4 cut(s) 167, 403, 463, 469
Hpy8I GTNNAC 3 cut(s) 46, 159, 440
Hpy99I CGWCG 2 cut(s) 47, 50
HpyAV CCTTC 2 cut(s) 44, 247
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 3 cut(s) 9, 93, 366
HpyCH4V TGCA 4 cut(s) 67, 98, 270, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 233
HpySE526I ACGT 3 cut(s) 9, 93, 366
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 3 cut(s) 67, 71, 389
HspAI GCGC 2 cut(s) 71, 225
Kzo9I GATC 4 cut(s) 30, 286, 319, 465
LmnI GCTCC 1 cut(s) 495
LpnPI CCDG 6 cut(s) 84, 98, 293, 297, 353, 471
MaeI CTAG 1 cut(s) 167
MaeII ACGT 3 cut(s) 9, 93, 366
MalI GATC 4 cut(s) 32, 288, 321, 467
MbiI CCGCTC 3 cut(s) 25, 315, 352
MboI GATC 4 cut(s) 30, 286, 319, 465
MboII GAAGA 3 cut(s) 100, 274, 484
MflI RGATCY 2 cut(s) 30, 319
MhlI GDGCHC 1 cut(s) 442
MluCI AATT 1 cut(s) 106
MnlI CCTC 9 cut(s) 21, 44, 49, 137, 140, 252, 287, 318, 399
Mph1103I ATGCAT 1 cut(s) 69
MseI TTAA 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 390
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 1 cut(s) 493
MvnI CGCG 2 cut(s) 148, 236
MwoI GCNNNNNNNGC 2 cut(s) 60, 233
NciI CCSGG 1 cut(s) 493
NcoI CCATGG 1 cut(s) 385
NdeII GATC 4 cut(s) 30, 286, 319, 465
NlaIII CATG 3 cut(s) 67, 71, 389
NlaIV GGNNCC 2 cut(s) 32, 83
NsiI ATGCAT 1 cut(s) 69
NspI RCATGY 2 cut(s) 67, 71
PaeI GCATGC 2 cut(s) 67, 71
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 51, 240
PkrI GCNGC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 274
Psp1406I AACGTT 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 32, 83
PspPI GGNCC 1 cut(s) 274
PspPPI RGGWCCY 1 cut(s) 274
PsrI GAACNNNNNNTAC 2 cut(s) 339, 371
PsuI RGATCY 2 cut(s) 30, 319
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 390
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 4 cut(s) 30, 286, 319, 465
Sau96I GGNCC 1 cut(s) 274
ScrFI CCNGG 1 cut(s) 493
SduI GDGCHC 1 cut(s) 442
SetI ASST 5 cut(s) 12, 96, 154, 279, 369
SgrDI CGTCGACG 1 cut(s) 44
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 2 cut(s) 67, 71
Sse9I AATT 1 cut(s) 106
SsiI CCGC 7 cut(s) 25, 146, 206, 236, 296, 315, 352
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 491
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 3 cut(s) 12, 96, 369
TaqI TCGA 1 cut(s) 45
TasI AATT 1 cut(s) 106
TauI GCSGC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 440
Vha464I CTTAAG 1 cut(s) 290
VneI GTGCAC 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 274
XapI RAATTY 1 cut(s) 106
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 2 cut(s) 67, 71
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.