MD05G1045100.v1.1

CD2 antigen cytoplasmic tail-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003252
Physical Location & Seq
Reverse (-)
7742326 .. 7748393
6068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1045100.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGAAGGCAATCGGAATTTGAAACGTCCCTTCTTCGAAGACGACAGCTCCGGCAAACCTCCGGCGCAAAAGAGGGTTAGGTTCCCCAAGGGGAAGAAGGCGAGGCCGGAAGATGAAACGGCACTCAAATCCATACCTGCTGAGGCCATTGGGCCGACTCCGGCGGCGGCGGCGACGGACCCTCGGGCCGCCGCCAAAGAGCGTGCCAAGCGCCGCAGCCAAATCACAACTGAGCTCTTCACTGAAGACGCTGCAGGCGTTGTCAGCGATGTCTCTGCTGCCGAAGTTACTTACAAGGATTATGAGGACTTTGTTGAGGATGGGATTCGAATTGAACCTTTCAACTTGAACAAGGAGAGGGAAGAAGGTTATTTTGATGCAAATGGAAATTTCGTTGAATATGCCAATAAAAATGAAGTCAAGGATGCATGGCTTGATAGTATTGAAGTTGCTACAAAATATGCTGGAAAAGACGTTGTAGTAACAAAAGATTATGATGATGTCCAAGATTTATCTTCTGATGACATTGGGAAGATAAAAAGACGTATTGCTGATGTGCTTGAACCAGGAGAAACGGTTCTACACGCTTTGAGAAGATTGAAGGGAACACCTAAAAACAAGAAGGAGAAGATGTCAGCTGAAACGAAGATAGTATTTGACCAGTTAACTGAAGATGCCATGAACCTGATGGATAACGGTGAATCCAATGTTTATCATGAGAAGAAGGAGATTTTTGAGCGTGAGGCAGAAGGATATGAGAGTTTAGCTCGGGCAAGAAGGGAACCTACATCTATTAGTGCATATCAAGAGAGATCTGTTCTCGGCATGGAGTGGGAGTCCTCCTCTGATGTAACAAATCCTGGAGCACCCTCTTCAATACTGCCGGAAACTGCTGTGGCCACATCCAGTTCTAACGCAACCACTGTAGAAATCTCAAGTAACGGTGATGATGCATTTGATATGTTTGCAGAAGATGATGAACATGCAATTGCTAAACCTTCTGAAGGAAGTAATGTGATTTCTGGCCCTAATTCTTATTGCGTCAGCTCACCGTCATCGAACAACCTAAACAATTATTCAGAGAATGGAGTTTTGCAAAATGATTATGTGTTTGATGAGTCTTCTGGGTACTATTATAGCAGCAGTTTGGGATATTATTATGACCCATCTACAGGACTCTATTGCTCCGCTTCATCAGGGCTATGGTACTCGTTCAATGAGGAAACTGGCATATATGATGAGATTCACCAGACTACAACCACTGTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

424

Amino Acids

46.77

Weight (kDa)

4.58

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OCRE PF17780 366 - 414 4.3e-16 OCRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 145
AciI CCGC 7 cut(s) 164, 167, 170, 189, 192, 214, 1188
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 2 cut(s) 16, 388
AcuI CTGAAG 3 cut(s) 264, 690, 1023
AfaI GTAC 2 cut(s) 1130, 1208
AfiI CCNNNNNNNGG 2 cut(s) 1004, 1172
AjnI CCWGG 2 cut(s) 565, 859
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 5 cut(s) 48, 235, 638, 767, 1047
AluI AGCT 5 cut(s) 48, 235, 638, 767, 1047
Alw21I GWGCWC 2 cut(s) 237, 868
Alw26I GTCTC 1 cut(s) 277
Ama87I CYCGRG 2 cut(s) 183, 768
AoxI GGCC 6 cut(s) 104, 144, 152, 186, 897, 1024
ApeKI GCWGC 4 cut(s) 216, 251, 278, 1140
ApoI RAATTY 2 cut(s) 16, 388
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 2 cut(s) 67, 213
AspS9I GGNCC 4 cut(s) 152, 178, 186, 1025
AsuHPI GGTGA 4 cut(s) 710, 956, 1041, 1238
AsuII TTCGAA 2 cut(s) 36, 328
AvaI CYCGRG 2 cut(s) 183, 768
AvaII GGWCC 1 cut(s) 178
BalI TGGCCA 1 cut(s) 899
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 769, 801
BbsI GAAGAC 3 cut(s) 45, 252, 1113
Bbv12I GWGCWC 2 cut(s) 237, 868
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 4 cut(s) 228, 238, 265, 1152
BccI CCATC 3 cut(s) 314, 682, 1174
BceAI ACGGC 1 cut(s) 135
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 2 cut(s) 567, 861
BcoDI GTCTC 1 cut(s) 277
BfmI CTRYAG 3 cut(s) 252, 924, 1170
BfoI RGCGCY 1 cut(s) 214
BfuAI ACCTGC 1 cut(s) 145
BglII AGATCT 1 cut(s) 812
Bme1390I CCNGG 2 cut(s) 567, 861
Bme18I GGWCC 1 cut(s) 178
BmeT110I CYCGRG 2 cut(s) 183, 768
BmgT120I GGNCC 4 cut(s) 152, 178, 186, 1025
BmiI GGNNCC 3 cut(s) 83, 180, 783
BmrFI CCNGG 2 cut(s) 567, 861
BmsI GCATC 4 cut(s) 367, 415, 664, 940
BpiI GAAGAC 3 cut(s) 45, 252, 1113
BplI GAGNNNNNCTC 4 cut(s) 751, 783, 827, 859
BpmI CTGGAG 1 cut(s) 882
Bpu10I CCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 2 cut(s) 36, 328
BpuEI CTTGAG 1 cut(s) 919
BsaJI CCNNGG 2 cut(s) 87, 182
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 561, 591, 617, 647, 821, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 1004, 1172
Bse1I ACTGG 3 cut(s) 661, 906, 1231
BseBI CCWGG 2 cut(s) 567, 861
BseDI CCNNGG 2 cut(s) 87, 182
BseGI GGATG 3 cut(s) 325, 430, 902
BseLI CCNNNNNNNGG 2 cut(s) 1004, 1172
BseMII CTCAG 2 cut(s) 132, 222
BseNI ACTGG 3 cut(s) 661, 906, 1231
BseRI GAGGAG 1 cut(s) 832
BseXI GCAGC 4 cut(s) 228, 238, 265, 1152
BshFI GGCC 6 cut(s) 106, 146, 154, 188, 899, 1026
BsiHKAI GWGCWC 2 cut(s) 237, 868
BsiHKCI CYCGRG 2 cut(s) 183, 768
BsiSI CCGG 5 cut(s) 51, 62, 107, 161, 884
BslFI GGGAC 1 cut(s) 12
BslI CCNNNNNNNGG 2 cut(s) 1004, 1172
BsmAI GTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 12
BsnI GGCC 6 cut(s) 106, 146, 154, 188, 899, 1026
BsoBI CYCGRG 2 cut(s) 183, 768
Bsp119I TTCGAA 2 cut(s) 36, 328
Bsp1286I GDGCHC 2 cut(s) 237, 868
Bsp143I GATC 1 cut(s) 812
BspACI CCGC 7 cut(s) 164, 167, 170, 189, 192, 214, 1188
BspANI GGCC 6 cut(s) 106, 146, 154, 188, 899, 1026
BspCNI CTCAG 2 cut(s) 133, 223
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 3 cut(s) 83, 180, 783
BspMAI CTGCAG 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 145
BspQI GCTCTTC 1 cut(s) 242
BspT104I TTCGAA 2 cut(s) 36, 328
BsrI ACTGG 3 cut(s) 661, 906, 1231
BssECI CCNNGG 2 cut(s) 87, 182
BssMI GATC 1 cut(s) 812
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 567, 861
Bst4CI ACNGT 6 cut(s) 577, 698, 925, 944, 1053, 1264
Bst6I CTCTTC 2 cut(s) 242, 877
BstBI TTCGAA 2 cut(s) 36, 328
BstC8I GCNNGC 2 cut(s) 204, 256
BstDEI CTNAG 2 cut(s) 141, 231
BstENI CCTNNNNNAGG 1 cut(s) 1002
BstF5I GGATG 3 cut(s) 325, 430, 902
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 2 cut(s) 67, 213
BstKTI GATC 1 cut(s) 815
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 1 cut(s) 812
BstMWI GCNNNNNNNGC 3 cut(s) 170, 208, 264
BstNI CCWGG 2 cut(s) 567, 861
BstNSI RCATGY 1 cut(s) 986
BstSCI CCNGG 2 cut(s) 565, 859
BstSFI CTRYAG 3 cut(s) 252, 924, 1170
BstV1I GCAGC 4 cut(s) 228, 238, 265, 1152
BstV2I GAAGAC 3 cut(s) 45, 252, 1113
BstX2I RGATCY 1 cut(s) 812
BstYI RGATCY 1 cut(s) 812
BsuRI GGCC 6 cut(s) 106, 146, 154, 188, 899, 1026
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 325, 430, 902
BtsIMutI CAGTG 3 cut(s) 240, 921, 1260
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 204, 256
CciI TCATGA 1 cut(s) 715
CfoI GCGC 2 cut(s) 67, 213
Cfr13I GGNCC 4 cut(s) 152, 178, 186, 1025
CseI GACGC 2 cut(s) 257, 1030
Csp6I GTAC 2 cut(s) 1129, 1207
CviAII CATG 5 cut(s) 429, 679, 716, 826, 983
CviQI GTAC 2 cut(s) 1129, 1207
DdeI CTNAG 2 cut(s) 141, 231
DpnI GATC 1 cut(s) 814
DpnII GATC 1 cut(s) 812
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 2 cut(s) 242, 877
EarI CTCTTC 2 cut(s) 242, 877
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 3 cut(s) 264, 690, 1023
Eco88I CYCGRG 2 cut(s) 183, 768
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 1002
EcoRII CCWGG 2 cut(s) 565, 859
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 2 cut(s) 430, 955
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 432, 682, 719, 829, 986
FaqI GGGAC 1 cut(s) 12
FatI CATG 5 cut(s) 428, 678, 715, 825, 982
FokI GGATG 3 cut(s) 332, 437, 889
FriOI GRGCYC 1 cut(s) 237
GlaI GCGC 2 cut(s) 66, 212
GsuI CTGGAG 1 cut(s) 882
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 6 cut(s) 106, 146, 154, 188, 899, 1026
HapII CCGG 5 cut(s) 51, 62, 107, 161, 884
HgaI GACGC 2 cut(s) 257, 1030
HhaI GCGC 2 cut(s) 67, 213
Hin1II CATG 5 cut(s) 432, 682, 719, 829, 986
Hin6I GCGC 2 cut(s) 65, 211
HinP1I GCGC 2 cut(s) 65, 211
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 7 cut(s) 157, 325, 701, 836, 1118, 1176, 1243
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 5 cut(s) 51, 62, 107, 161, 884
HphI GGTGA 4 cut(s) 710, 956, 1041, 1238
Hpy166II GTNNAC 1 cut(s) 666
Hpy188I TCNGA 5 cut(s) 15, 520, 847, 1003, 1081
Hpy188III TCNNGA 2 cut(s) 716, 806
Hpy8I GTNNAC 1 cut(s) 666
Hpy99I CGWCG 1 cut(s) 178
HpyCH4III ACNGT 6 cut(s) 577, 698, 925, 944, 1053, 1264
HpyCH4IV ACGT 3 cut(s) 25, 474, 544
HpyCH4V TGCA 8 cut(s) 254, 380, 428, 800, 953, 968, 986, 1096
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 208, 264
HpyF3I CTNAG 2 cut(s) 141, 231
HpySE526I ACGT 3 cut(s) 25, 474, 544
Hsp92II CATG 5 cut(s) 432, 682, 719, 829, 986
HspAI GCGC 2 cut(s) 65, 211
KspAI GTTAAC 1 cut(s) 666
Kzo9I GATC 1 cut(s) 812
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 3 cut(s) 53, 863, 1190
Lsp1109I GCAGC 4 cut(s) 228, 238, 265, 1152
LweI GCATC 4 cut(s) 367, 415, 664, 940
MaeII ACGT 3 cut(s) 25, 474, 544
MaeIII GTNAC 4 cut(s) 286, 481, 850, 938
MalI GATC 1 cut(s) 814
MboI GATC 1 cut(s) 812
MfeI CAATTG 1 cut(s) 987
MflI RGATCY 1 cut(s) 812
MhlI GDGCHC 2 cut(s) 237, 868
MlsI TGGCCA 1 cut(s) 899
MluCI AATT 6 cut(s) 16, 330, 388, 987, 1030, 1072
MluNI TGGCCA 1 cut(s) 899
MlyI GAGTC 4 cut(s) 151, 845, 1127, 1170
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 2 cut(s) 430, 955
MroXI GAANNNNTTC 1 cut(s) 576
MscI TGGCCA 1 cut(s) 899
MseI TTAA 1 cut(s) 665
Msp20I TGGCCA 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 5 cut(s) 51, 62, 107, 161, 884
MspR9I CCNGG 2 cut(s) 567, 861
MunI CAATTG 1 cut(s) 987
MvaI CCWGG 2 cut(s) 567, 861
MwoI GCNNNNNNNGC 3 cut(s) 170, 208, 264
NdeII GATC 1 cut(s) 812
NlaIII CATG 5 cut(s) 432, 682, 719, 829, 986
NlaIV GGNNCC 3 cut(s) 83, 180, 783
NmeAIII GCCGAG 1 cut(s) 801
NsiI ATGCAT 2 cut(s) 430, 955
NspI RCATGY 1 cut(s) 986
NspV TTCGAA 2 cut(s) 36, 328
PagI TCATGA 1 cut(s) 715
PciSI GCTCTTC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 3 cut(s) 325, 701, 1243
PfoI TCCNGGA 1 cut(s) 859
PleI GAGTC 4 cut(s) 151, 844, 1126, 1170
PpsI GAGTC 4 cut(s) 151, 844, 1126, 1170
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 2 cut(s) 565, 859
PspGI CCWGG 2 cut(s) 565, 859
PspN4I GGNNCC 3 cut(s) 83, 180, 783
PspPI GGNCC 4 cut(s) 152, 178, 186, 1025
PstI CTGCAG 1 cut(s) 256
PsuI RGATCY 1 cut(s) 812
PvuII CAGCTG 1 cut(s) 638
RsaI GTAC 2 cut(s) 1130, 1208
RsaNI GTAC 2 cut(s) 1129, 1207
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 1 cut(s) 665
Sau3AI GATC 1 cut(s) 812
Sau96I GGNCC 4 cut(s) 152, 178, 186, 1025
SchI GAGTC 4 cut(s) 151, 845, 1127, 1170
ScrFI CCNGG 2 cut(s) 567, 861
SduI GDGCHC 2 cut(s) 237, 868
SfaNI GCATC 4 cut(s) 367, 415, 664, 940
SfcI CTRYAG 3 cut(s) 252, 924, 1170
SfuI TTCGAA 2 cut(s) 36, 328
SinI GGWCC 1 cut(s) 178
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
Sse9I AATT 6 cut(s) 16, 330, 388, 987, 1030, 1072
SsiI CCGC 7 cut(s) 164, 167, 170, 189, 192, 214, 1188
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 565, 859
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 6 cut(s) 577, 698, 925, 944, 1053, 1264
TaiI ACGT 3 cut(s) 28, 477, 547
TaqI TCGA 3 cut(s) 36, 328, 1058
TasI AATT 6 cut(s) 16, 330, 388, 987, 1030, 1072
TauI GCSGC 6 cut(s) 167, 170, 173, 191, 194, 216
TfiI GAWTC 3 cut(s) 325, 701, 1243
Tru1I TTAA 1 cut(s) 665
Tru9I TTAA 1 cut(s) 665
TscAI CASTG 3 cut(s) 247, 928, 1267
TseI GCWGC 4 cut(s) 216, 251, 278, 1140
TspDTI ATGAA 5 cut(s) 129, 429, 695, 993, 1182
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 3 cut(s) 247, 928, 1267
VpaK11BI GGWCC 1 cut(s) 178
XagI CCTNNNNNAGG 1 cut(s) 1002
XapI RAATTY 2 cut(s) 16, 388
XceI RCATGY 1 cut(s) 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 576
Zsp2I ATGCAT 2 cut(s) 430, 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.