MD14G1166200.v1.1

CD2 antigen cytoplasmic tail-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003252
Physical Location & Seq
Reverse (-)
25975839 .. 25976981
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1166200.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGAACCCAATCTTCCATCGCGATCCAATCGCAAGCGCCCCGTCCCCGAACTCTCCGACTCCGATTCCGATTCCGACAGACCCACGGCGGAAAGGAGGGTTAAGTTTCCCAAGGGGAAGAAGGCGAGACGGGAAGACGAACCCGTGAAGATACCCCGAGCCGACGAGGACGACGACGAGATCGGGGTTGAGTTTCCGGACCCCCGTTTCGCCGCCAAAGAACGCGAGAAGCGGCGCCGGTTCGGGGACGACGGGATCGGCGGCGACGATGTCTCCGCCGCCGAAGTCGCGTACGGCGAGGAATTGGATTACGACAATTTCGTGGAGGACGGGATTCAGATGGAGCCTTTCAACTTGGAGAAGGAGAGGAAAGAGGGGTATTTCGATGCCGAGGGGAATTTCGTCGAGTATGCTGAAGGAAATCGAGTGAAGGATGCTTGGCTGGATAGCGTTGAAGCTGGGGTTACGCAGTTACATGTTCCGAAACCGAAAGTCGAAGATGATACGGAGGATTTGCAACCGGAAGATGAAGATATTGGGAAGATTAAGAGGCGCATTGCCGATGTGCTCGAGGAAGGCGAAACGGTGTTGCAGGCTCTGAGGAGGTTGAAGGGGGACAAGAAGGTGAAGATGTCGCCGGAGACGAAGAGGGTTTTCGATCAGCTGACTGAGGATGCTGTGACACTGATGGACAAGCATGGGGAGTATGATGTGAATCATGAGAAGAAGGAGATTTTCGAACGGGAGGCTCAGGGGTATGAGACTTTAGCTAGGGCAAGACTCGAAGGCATCCCGCCAGTTGCTAACGATAACAATGGTGATGAAACTGCTGGAGATGGATATGATATGTTTGGTGAAGACGATGATGAAGATGCTAATGCTGCTGCTAGACCGTCGGATTCCAACGGAAGAGGAGTTGGTTCGTCCGAAGCGCAGAATGATTATGTGTTTGATGAGAATTCGGGGTACTATTATAGCAGCAGTTTGGGGTACTATTATGATCCGTCGACAGGGCTCTATTGCTCCGCTGCATCTGGGGTTTGGTACTCGTACAATGAGGAGACCGGAGCGTATGACGAAGTTTCTCACCATGATCAGGCTGCTTCTGCTGCGGCTGCCACCGCTGAGGCTGCAAAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

42.5

Weight (kDa)

4.44

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OCRE PF17780 312 - 362 4.4e-19 OCRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 1007
AccII CGCG 3 cut(s) 22, 225, 290
AccIII TCCGGA 1 cut(s) 196
AclWI GGATC 3 cut(s) 18, 263, 995
AcsI RAATTY 2 cut(s) 397, 958
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 5 cut(s) 293, 968, 992, 1046, 1052
AfiI CCNNNNNNNGG 3 cut(s) 244, 1010, 1096
AflIII ACRYGT 1 cut(s) 475
AgsI TTSAA 3 cut(s) 352, 455, 610
AluBI AGCT 3 cut(s) 458, 664, 770
AluI AGCT 3 cut(s) 458, 664, 770
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 5 cut(s) 121, 277, 635, 755, 1055
AlwI GGATC 3 cut(s) 18, 263, 995
AlwNI CAGNNNCTG 1 cut(s) 598
Ama87I CYCGRG 2 cut(s) 156, 569
Aor13HI TCCGGA 1 cut(s) 196
ApeKI GCWGC 8 cut(s) 881, 884, 978, 1028, 1100, 1109, 1115, 1130
ApoI RAATTY 2 cut(s) 397, 958
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 4 cut(s) 39, 237, 555, 934
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 4 cut(s) 637, 830, 866, 1079
AsuII TTCGAA 1 cut(s) 738
AvaI CYCGRG 2 cut(s) 156, 569
AvaII GGWCC 1 cut(s) 199
BanI GGYRCC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 1017
BbsI GAAGAC 2 cut(s) 141, 864
Bbv12I GWGCWC 1 cut(s) 570
BbvCI CCTCAGC 1 cut(s) 1125
BbvI GCAGC 8 cut(s) 868, 871, 990, 1015, 1087, 1096, 1102, 1117
BccI CCATC 4 cut(s) 25, 334, 682, 830
BceAI ACGGC 2 cut(s) 102, 310
BclI TGATCA 1 cut(s) 1093
BcoDI GTCTC 5 cut(s) 121, 277, 635, 755, 1055
BfaI CTAG 2 cut(s) 771, 888
BfoI RGCGCY 2 cut(s) 40, 238
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 2 cut(s) 156, 569
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 4 cut(s) 6, 201, 236, 345
BmsI GCATC 6 cut(s) 376, 424, 664, 798, 862, 1040
BpiI GAAGAC 2 cut(s) 141, 864
BpmI CTGGAG 1 cut(s) 852
Bpu10I CCTNAGC 2 cut(s) 750, 1125
Bpu14I TTCGAA 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 714
BsaHI GRCGYC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 1055
BsaJI CCNNGG 3 cut(s) 84, 111, 390
BsaWI WCCGGW 3 cut(s) 196, 520, 1064
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 1010, 1096
Bse118I RCCGGY 1 cut(s) 237
Bse1I ACTGG 1 cut(s) 797
Bse3DI GCAATG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 714
BseAI TCCGGA 1 cut(s) 196
BseDI CCNNGG 3 cut(s) 84, 111, 390
BseGI GGATG 3 cut(s) 439, 679, 789
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 3 cut(s) 244, 1010, 1096
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 4 cut(s) 590, 660, 764, 1116
BseNI ACTGG 1 cut(s) 797
BseRI GAGGAG 3 cut(s) 616, 927, 1073
BseXI GCAGC 8 cut(s) 868, 871, 990, 1015, 1087, 1096, 1102, 1117
BseYI CCCAGC 1 cut(s) 458
Bsh1236I CGCG 3 cut(s) 22, 225, 290
BshNI GGYRCC 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 2 cut(s) 156, 569
BsiSI CCGG 5 cut(s) 197, 238, 521, 638, 1065
BsiWI CGTACG 1 cut(s) 291
BslFI GGGAC 3 cut(s) 29, 260, 629
BslI CCNNNNNNNGG 3 cut(s) 244, 1010, 1096
BsmAI GTCTC 5 cut(s) 121, 277, 635, 755, 1055
BsmBI CGTCTC 2 cut(s) 121, 635
BsmFI GGGAC 3 cut(s) 29, 260, 629
Bso31I GGTCTC 1 cut(s) 1055
BsoBI CYCGRG 2 cut(s) 156, 569
Bsp119I TTCGAA 1 cut(s) 738
Bsp1286I GDGCHC 2 cut(s) 570, 1017
Bsp13I TCCGGA 1 cut(s) 196
Bsp143I GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
Bsp68I TCGCGA 1 cut(s) 22
BspCNI CTCAG 4 cut(s) 591, 661, 763, 1117
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 3 cut(s) 22, 225, 290
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 4 cut(s) 6, 201, 236, 345
BspPI GGATC 3 cut(s) 18, 263, 995
BspT104I TTCGAA 1 cut(s) 738
BspT107I GGYRCC 1 cut(s) 234
BspTNI GGTCTC 1 cut(s) 1055
BsrDI GCAATG 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 237
BsrI ACTGG 1 cut(s) 797
BssAI RCCGGY 1 cut(s) 237
BssECI CCNNGG 3 cut(s) 84, 111, 390
BssMI GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
BssNI GRCGYC 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 586, 894
Bst6I CTCTTC 2 cut(s) 641, 904
BstACI GRCGYC 1 cut(s) 235
BstBI TTCGAA 1 cut(s) 738
BstC8I GCNNGC 2 cut(s) 35, 594
BstDEI CTNAG 4 cut(s) 599, 669, 750, 1125
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 3 cut(s) 439, 679, 789
BstFNI CGCG 3 cut(s) 22, 225, 290
BstH2I RGCGCY 2 cut(s) 40, 238
BstHHI GCGC 4 cut(s) 39, 237, 555, 934
BstKTI GATC 6 cut(s) 26, 183, 258, 661, 1003, 1096
BstMAI GTCTC 5 cut(s) 121, 277, 635, 755, 1055
BstMBI GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
BstMWI GCNNNNNNNGC 7 cut(s) 287, 881, 1106, 1109, 1115, 1121, 1130
BstNSI RCATGY 1 cut(s) 479
BstUI CGCG 3 cut(s) 22, 225, 290
BstV1I GCAGC 8 cut(s) 868, 871, 990, 1015, 1087, 1096, 1102, 1117
BstV2I GAAGAC 2 cut(s) 141, 864
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 3
BtsCI GGATG 3 cut(s) 439, 679, 789
BtsIMutI CAGTG 1 cut(s) 683
BtuMI TCGCGA 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 35, 594
CaiI CAGNNNCTG 1 cut(s) 598
CciI TCATGA 1 cut(s) 718
CfoI GCGC 4 cut(s) 39, 237, 555, 934
Cfr10I RCCGGY 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 5 cut(s) 292, 967, 991, 1045, 1051
CviAII CATG 4 cut(s) 476, 698, 719, 1091
CviQI GTAC 5 cut(s) 292, 967, 991, 1045, 1051
DdeI CTNAG 4 cut(s) 599, 669, 750, 1125
DinI GGCGCC 1 cut(s) 236
DpnI GATC 6 cut(s) 25, 182, 257, 660, 1002, 1095
DpnII GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
Eam1104I CTCTTC 2 cut(s) 641, 904
EarI CTCTTC 2 cut(s) 641, 904
EciI GGCGGA 2 cut(s) 104, 265
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 1017
Eco31I GGTCTC 1 cut(s) 1055
Eco47I GGWCC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 2 cut(s) 156, 569
EcoRI GAATTC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 1017
EgeI GGCGCC 1 cut(s) 236
EheI GGCGCC 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 111
Esp3I CGTCTC 2 cut(s) 121, 635
FaeI CATG 4 cut(s) 479, 701, 722, 1094
FaqI GGGAC 3 cut(s) 29, 260, 629
FatI CATG 4 cut(s) 475, 697, 718, 1090
FauI CCCGC 1 cut(s) 801
FbaI TGATCA 1 cut(s) 1093
FblI GTMKAC 1 cut(s) 1007
FokI GGATG 3 cut(s) 446, 686, 776
FriOI GRGCYC 1 cut(s) 1017
FspBI CTAG 2 cut(s) 771, 888
GlaI GCGC 4 cut(s) 38, 236, 554, 933
GsaI CCCAGC 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 852
HaeII RGCGCY 2 cut(s) 40, 238
HapII CCGG 5 cut(s) 197, 238, 521, 638, 1065
HhaI GCGC 4 cut(s) 39, 237, 555, 934
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 4 cut(s) 479, 701, 722, 1094
Hin6I GCGC 4 cut(s) 37, 235, 553, 932
HinP1I GCGC 4 cut(s) 37, 235, 553, 932
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HinfI GANTC 7 cut(s) 59, 65, 71, 334, 715, 780, 899
HpaII CCGG 5 cut(s) 197, 238, 521, 638, 1065
HphI GGTGA 4 cut(s) 637, 830, 866, 1079
Hpy166II GTNNAC 1 cut(s) 1008
Hpy188I TCNGA 9 cut(s) 58, 64, 70, 76, 339, 483, 600, 898, 928
Hpy188III TCNNGA 3 cut(s) 21, 197, 719
Hpy8I GTNNAC 1 cut(s) 1008
Hpy99I CGWCG 8 cut(s) 167, 176, 179, 254, 269, 407, 898, 1009
HpyAV CCTTC 9 cut(s) 115, 355, 410, 424, 569, 604, 616, 721, 779
HpyCH4III ACNGT 2 cut(s) 586, 894
HpyCH4V TGCA 4 cut(s) 517, 592, 1031, 1133
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 881, 1106, 1109, 1115, 1121, 1130
HpyF3I CTNAG 4 cut(s) 599, 669, 750, 1125
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 4 cut(s) 479, 701, 722, 1094
HspAI GCGC 4 cut(s) 37, 235, 553, 932
KasI GGCGCC 1 cut(s) 234
Kpn2I TCCGGA 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 1093
Kzo9I GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
LmnI GCTCC 3 cut(s) 343, 1028, 1067
Lsp1109I GCAGC 8 cut(s) 868, 871, 990, 1015, 1087, 1096, 1102, 1117
LweI GCATC 6 cut(s) 376, 424, 664, 798, 862, 1040
MaeI CTAG 2 cut(s) 771, 888
MaeIII GTNAC 3 cut(s) 463, 471, 679
MalI GATC 6 cut(s) 25, 182, 257, 660, 1002, 1095
MboI GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
MhlI GDGCHC 2 cut(s) 570, 1017
MluCI AATT 4 cut(s) 302, 316, 397, 958
Mly113I GGCGCC 1 cut(s) 235
MlyI GAGTC 2 cut(s) 53, 774
MmeI TCCRAC 4 cut(s) 81, 99, 876, 927
MroI TCCGGA 1 cut(s) 196
MseI TTAA 2 cut(s) 102, 546
MspA1I CMGCKG 3 cut(s) 664, 1028, 1124
MspI CCGG 5 cut(s) 197, 238, 521, 638, 1065
MvnI CGCG 3 cut(s) 22, 225, 290
MwoI GCNNNNNNNGC 7 cut(s) 287, 881, 1106, 1109, 1115, 1121, 1130
NarI GGCGCC 1 cut(s) 235
NdeII GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
NlaIII CATG 4 cut(s) 479, 701, 722, 1094
NlaIV GGNNCC 4 cut(s) 6, 201, 236, 345
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 1 cut(s) 679
NruI TCGCGA 1 cut(s) 22
NspI RCATGY 1 cut(s) 479
NspV TTCGAA 1 cut(s) 738
PaeR7I CTCGAG 1 cut(s) 569
PagI TCATGA 1 cut(s) 718
PciI ACATGT 1 cut(s) 475
PcsI WCGNNNNNNNCGW 6 cut(s) 171, 264, 294, 318, 576, 641
PfeI GAWTC 5 cut(s) 65, 71, 334, 715, 899
Pfl23II CGTACG 1 cut(s) 291
PflFI GACNNNGTC 1 cut(s) 269
PleI GAGTC 2 cut(s) 53, 774
PluTI GGCGCC 1 cut(s) 238
PpsI GAGTC 2 cut(s) 53, 774
PscI ACATGT 1 cut(s) 475
PspFI CCCAGC 1 cut(s) 458
PspLI CGTACG 1 cut(s) 291
PspN4I GGNNCC 4 cut(s) 6, 201, 236, 345
PspPI GGNCC 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 569
PstNI CAGNNNCTG 1 cut(s) 598
PsyI GACNNNGTC 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 664
RruI TCGCGA 1 cut(s) 22
RsaI GTAC 5 cut(s) 293, 968, 992, 1046, 1052
RsaNI GTAC 5 cut(s) 292, 967, 991, 1045, 1051
SalI GTCGAC 1 cut(s) 1006
SaqAI TTAA 2 cut(s) 102, 546
Sau3AI GATC 6 cut(s) 23, 180, 255, 658, 1000, 1093
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 2 cut(s) 53, 774
SduI GDGCHC 2 cut(s) 570, 1017
SetI ASST 5 cut(s) 460, 608, 627, 666, 772
SfaNI GCATC 6 cut(s) 376, 424, 664, 798, 862, 1040
SfoI GGCGCC 1 cut(s) 236
Sfr274I CTCGAG 1 cut(s) 569
SfuI TTCGAA 1 cut(s) 738
SinI GGWCC 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 4 cut(s) 302, 316, 397, 958
SspDI GGCGCC 1 cut(s) 234
SspMI CTAG 2 cut(s) 771, 888
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 2 cut(s) 586, 894
TaqI TCGA 9 cut(s) 384, 405, 424, 495, 570, 657, 738, 783, 1007
TasI AATT 4 cut(s) 302, 316, 397, 958
TauI GCSGC 5 cut(s) 215, 235, 264, 281, 1115
TfiI GAWTC 5 cut(s) 65, 71, 334, 715, 899
Tru1I TTAA 2 cut(s) 102, 546
Tru9I TTAA 2 cut(s) 102, 546
TscAI CASTG 1 cut(s) 690
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 8 cut(s) 881, 884, 978, 1028, 1100, 1109, 1115, 1130
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 3 cut(s) 543, 837, 882
TspGWI ACGGA 3 cut(s) 521, 921, 993
TspRI CASTG 1 cut(s) 690
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 2 cut(s) 397, 958
XceI RCATGY 1 cut(s) 479
XhoI CTCGAG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 1007
XspI CTAG 2 cut(s) 771, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.