MD05G1240600.v1.1

Nuclear-pore

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003251
Physical Location & Seq
Forward (+)
37117000 .. 37137248
20249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1240600.v1.1.491

Sequence Viewer

Length: 6306 bp
ATGCCGCTCTTCGTCTCCGACGAGGACTTCTCCCGCCACGGCAACGACGCCGTCTGGGTGGCGGACAAGGCCGACGCCTATATTCGGGACCTGTACAAGGAGCTTGAGACCGTCAGGGCCCAGAACGACGCCGCTTCCATCACGGCCGAACAGACCTGCTCCCTCTTCGAGCAGAAGTACCTTTCCATCGCCGACGAATTCTCCAAGCTCGAGTCTCAATACGCGCAGCTTCAGTCCTCCCTCGACAGCCGCCTCTCCGAGGTCGCCGAGCTCCAGTCGCAGAAGCACCAGCTCCATCTCCAATCCATTGGGAAGGATGGGGAGACAGAGAGGATTAAGGCGGAGGTGTCGGAATTGCACAAATCGAAGAGGCAGTTGATAGAGTTGGTGGAGCAAAAGGACTTGGAAATTAGTGAAAAGAACGCCACCATCAAGTCCTATATCGACAGGATTGTATTATCTTCTGACAATGCTGCTCAAAGGGAGGCCCGTTTGAGCGAAGCGGAGGCAGAATTAGCACGTACCAAGGCTGCATGTACTCGTCTATCACAGGAGAAAGAACTTATTGAGAGGCATAATGTCTGGCTTAATGATGAGTTAACAGAGAAAGTTAACAGTCTCGTTCGGCTGCGTAGAACGCATGCTGATGTTGAGGCAGATTTGTCTTCTAAACTTGCAGATGTTGAGAGACAATTCAATGATTGCTCTAGCTCTTTAAAATGGCACAAGCAAAGAGTGGGGGAATTAGGCGACAGGCTGACGTCTTTACAGGAGGAGCTCCACTCATCTAAAGATGCTGCTGCAGCCAATGAAGAGCGATTAACCGCTGAACTCTCAACATTAAATAAGCTTGTTGAACTATACAAAGAAAGTTCTGAGGAATGGTCCAAAAAGGCAGGAGACCTTGAAGGTGTGATCAAGGCTCTGGAGACACATTTGAATCAAGAGGAAAATGATTACAAGGAAAGACTTGAAAGAGCAGAGTCTGCAAGAAATCAACTTGAGAAGGAAGCTGCAGATCTTAAAGTGAAACTTGAAAAATGTGAAGCAGAAATCGAAGCTAGCAGGAAATCAACTGAGCTTAATCTTCTTCCACTCAGTAGTTTTAGTACCGAAGCATGGATGAATTCATTTGAATCTACCGACATTATGGAGGCTGATCAAGCGGTTGTCCCAAGGATTCCTGCTGGTGTTTCAGGAACAGCATTAGCAGCTTCACTTCTGCGGGATGGCTGGAGTCTTGCAAAAATGTATGCTAAGTACCAAGAGGCTGTTGATGCCTTTCGTCATGAGCAGTTGGGAAGGAAGGAATCTGAGGCAGTCTTGCAACGGGTTCTATATGAATTAGAGGAGAAAGCAGAAGTCATTTTTGATGAAAGAGATGAACATGAGAGAATGGTTGAGGCATATTCTATGATCAACCAGAAATTGCAGAACTCGATTTCTGAACAGGCAAACTTGGAGAAGACCATCCAGGAGTTAAAGGCTGAGGTAAGGAGGCATGAGCGTGACTACACGTTTGCACGAAAAGAGATTGCTGATCTCCAGCGAGAGGTGACAATCCTTCTGAAGGAATGCCGTGATATACAGCTTCGTGGGACATCATCTGGTCATGATAGTCATGACTATGATACTGTCGCCGTTGTTGAGATGAACACTGAATCTGATGCCGAGAGAGTGATATCTGAACATCTTTTGACCTTCAAGGACATAAATGGACTAGTTGATCAGAATGTGCAGCTCAGGAGCCTTGTTCGGAATCTTTCTGACCAGCTTGAAAATAGGGAAATGGAGTTCAAGGAGAATTTTGAAATGGAGATCAAGAAGCACACTGATGAAGCTGCCTCTAGAGTTGCGGCAGTGTTACAAAGAGCTGAAGAGCAAGGGCGCATGATTGAATCCCTTCACACCTCTGTTGCAATGTACAAAAGGCTATATGAAGAGGAGCATAAACTTCATTCGTCTAGTCCTTGTATAGAAGAGGCTACTCCAGAAGAAAGAAGAACTGACGTCAAGCTTCTCTTTGAGAGTTCTCAGGAAGCTACAAGAAAGGCCCAAGACCAGACGGCAGAACGAGTGAAATGTCTGGAGGAAGATCTGGCAAGTACCAGGAGTGAGATCATATCGTTGCGGTCAGAGCGTGACAAGTTGGCTTTGGAAGCAAATTTTTCTCGAGAGAGACTTGAGAGCTTTATCAAAGAATTTGAGCATCAGAGAAATGAAACAAATGGTGTTTTAGCAAGAAACGTTGAGTTCTCTCAGTTGATAGTTGACTACCAACGAAGGCTGCGTGAAAGTTCTGAATCTGTTCAAACTGCTGAGGAGCGTACTAGGAAGTTGACTATGGAGGTGTCTGTTTTGAAGCATGAAAAGGAAATGTTGGAACATGCTGAGAAGCGTGCTTGTGACGAAGTTCGTAGTTTGTCGGAAAGGGTCCATAGACTGCAGGCCAGCTTGGACACTATTCAGAGTGCCGAGGAAGTTCGTGAGGAGGCAAGAGCTGCTGAGAGGAGAAGACAAGAAGAGTATACGAAACAAATAGAGAGGGAGTGGGCTGATGTCAAGAAAGATCTACAAGAAGAGCGAAACAATGCCCGTACTCTCACACTTGACCGTGAGCAGTCCGTACAAAATGCTATGAGACAAGTTGAAGAAATGGGAAAAGAGTTGGCTAATGCTTTGCATGCTGTTGCATCTGCTGAGACTAGAGCTGCTGTAGCCGAGGCTAAACTGACTGATCTGAACAAAAGGATTAAGTCCTCTGACGTTAAGGTTGTTGATGTCGATGGTGGGAGCGGATCCTCCTCCTTGACTAGTGATGAGGCTTTGGTGACATTGCGTGCTGCAAAGGAAGAGATTGAAAAGTTGAAAGAGGAAGTGCAAGCTAACAAGGATCACATGCTACAGTATAAGAGCATAGCTCAGGTAAATGAAGATGCATTGAGGCTGATGGAATCTGCCCATGAAAACTTCAAGATTGAGGCCGAAAAACTAAAGAAATCGCTTGAAGTCGAACTTCTTTCTCTAAGAGAGAGAGTATCTGAACTTGAACACGAGTGTAGTTTGAATTCTCAAGAAGTTGCTTCAGCGGCTGCAGGAAGGGAAGAAGCTCTTTCTTCCACTCTATCAGAAATTACAAGTTTAAAGGAAGAAACCTTAACTAAAACTTCCCAAATTGTGTCACTGGAGATTCAGATATCTGCTTTGAAAGAAGACTTGGAGAAAGAACGTCAGCGATCACGTTCTGCTCAGGCCAATTATGAAAGACAGGTTATCCTCCAGTCCGAGACAATACAGGAGTTGACCAAGACATCGCAAGATTTGGCTATGCTCCAGGAAGAAATGTCAGAGTTGCGTAAATTGGTTGATGCACTCAAAGGCGAAAATAATGAACTCAAGTCTAAGTGGGAATTTGAGAAGGCAATATTAGAAGAATCAAAAAATGTTGCCGAGAAGAAGTACATTGAAATCAACGAACAGAACAAAATACTGCACAGTCAACTTGAGGCTTTGCACATTCAACTGACTGAAAGAGATCGTGGTTCAGTTGGAACTTCTGCAATCACTGGTCCAGATATTTCAGGTGATGCTGGTTTACAAAATGTGATAAGTTATCTTCGACGGACAAAAGAAATTGCAGAGACAGAGATTTCCTTAATGAAACAAGAAAAGCTTCGATTACAATCACAACTTGAGAGTGCCCTCAAGGCTTCAGAAACAGCCCAGTCATCACTTCATGCCGAGCGCACTAATTCAAGATCATTGTTCACTGAGGAGGAAATGAAATCCTTGCAACTACAGGTTAGAGAAATGAACTTACTTCGTGAAAGCAATATCCAACTCAGAGAAGAAAACAAGCACAATTTTGAGGAATGCCAGAAATTCCGTGAAATATCACAAAAAGCTAGTGCTGAGACTGAGAACCTAGAGAGATTGCTACAGGAGAGGCAGATTGAGTTGGAAGCCTGTAAGAAAGAAATTGAAATGCAAAAGACAGAGAAAGAACACTCAGAACAGAGGGTTCGTGAGTTACTTGAAAGGTATAGAAATATTGATGTTCAAGATTATGATCGCGCAAAGGATGATGTTCGTCAGTTGCAGAAAAAATTGGAGGAGAAGGATTCTGAAATACTAGAAGTCAAGAAACTTCTTTCAGAAAAGATGGAGACTGTATCACGTCTGGAGCGAGATATTGCTAACTGCAGATTGGAACTGACTGAGATGGAGAAGAGAATGAGTGATGCTTTGCAAGCTGAGGCAAGTCTAAAATCAGAAATCGAGAAGCATAGGAAGATGGCCACTCAGTATAAGAGGAGGCTCGAAATGTTCTCAAGAGAAAAAGAGACCGTTTCAAAAGAAAAGGAGACACTGTCCAAGGAAAAGGAGGAACTGAGCAAGGAGAATCAAGCTCTTTCTAGACAGCTGGAGGAAGTCAAATTAGTGAAACGAGCATCAGTAGATACTACCGGGGTGCAAGCAATTAGGGAAGAGAAAGACCAGAAAATTCAGCTTCTGGAAAAGCATTTGGAGAGGCAAAGAGAGGAGCTGAGAAAGGAGAAGGATGAAAATCGGATGGAGAAAGCAATGCGCAAGAAGATGGAGAAGGCTGTAACAGATTCCTATAATAATGTTGACCAGGACAAGTCGAACTTCATGAATGAACTCGAGAAGCATAAGCAGGCTTTGAAGCAGCTTTCAGATGAACTTGAAAAGCTGAAACATGCAAAAGACAGTCTCCCTCAGGGTACCTCAATTGTTCAAATGCTTTCGGGAACCATACTGGATGGTCTTGCTGCTGCCTACGGTTTAGCTGTCGAAAATTTCGAAAAGACAGCACATTCAGTTCACAGTGAGTTTGGTGCTCACGGTGTTCTTGCAAACACTCCTCCAGTTGCAGATACTTCATTACCGGCTACCTCTGGTACCGCTCAAGCATCCACGGTTATGTCTTCAACGAGTCCTGCAAGGGGCTTAGTATCCAAAGCAACTGAAGAGAGGGGCTTAGCATCCACAGCCACTGAAGAAAGCGCAAAAACAATCACTTTACCCAAAGGCAATGTCGAAACCCGTAAACAAGGAAGGAGATTGGTTAGGCCCCGGCTTGTAAGACCTGAAGAACAACAAGGTGATGTAGAGATGTCAGAGATGGAAGGGACCCGCAATGGGGGCAAACAAGCACCATCGAATGAAATGGAAGTTCAAGGCAATGCTACTTTGCCACAACAGCTGCTTCGGAAACGTCTTGCTTCTTCATCTACTTTTGAGTCCCGTGAGGAATCAAATAACCAAGGGGAAATTTGTCCTGAGGTGGCAGCACCTGTTTCAAAGAAGGCCAAAGGTTCAGATTCTCTACAAGGGACTGAAGGGCAGCCATCTGCTATTTCGGAAAATCTTGGGAGTGTTCCAGTCAAAGATGAACCTTTAGACGTCGCTGTTGATTTGCCGCAAAGTTCAAGTGAGGAAGCTGCTGTTGATGCCGAGAAAGAAGAAACTGAGACTGCTGGGGAGAAGGTTGAAGAGCCAAATGAAAGACAGTTTGATGGTTTGAGTCAAGTTGAATCACAGAAGGATAGTGATTTAGTGGAGAATGTGGATGGATCAGATGGAAAAGATCTACCATCGCACGATGGAGCCAAAGATCAGGTTGAGCTGGAGCAACAGTCCAGTGATTTTGGAGGAGACCGAGAAGAGGGAGAGCTGGTCCCTGATATTTCTGAGCTTGAAGGTGGTGATGCGATGGGCAGTCCTGAAATAGGGGAAGTTCAACCTGAACCTGTAACAACTCCCGAGGCTTCCCCAGCTAGGGGCGATGATTACGGTGTTGCTGCTGGTTCTGTTGTGGATATCGGTGAGGTTAATTCTCCAGAGGTTCTTAATGATGAGAAAAATGATGACATTGATGCAACTGAAGAAGCTGCTGATGGTTCCGATAAGTCAATTGATGGTAATGACCATACTTTGACGGAGACTGACCAGGCTGCCGAAGCTACCTCAGTAATCGTAGATACCACTTCAACAAGTACCACAGCAGAAGTAAGCGTTTCAAAACAAGCTAGTCCCAGTGTTACTGCAGGAGAGGTGAGACAGGTGTCTCCTGTGATTATTCCGTCAACTACCATAAATTTAACAACACGAGCTCTGGAGAATGCACGCCGAAGACAACAGGCCAGAGGGCTCTCTACTTCACCAAGCACAGGTCGTGGAGGACCTGCCGGAAGACAAACTGGAAGATCTGGACGTGGACCTCCTGGACGTGGGCGTGGGCGCGGACGTTCTCGTGGGTCAACATCAGGCAATCAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000278 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005829 GO:0005911 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007088 GO:0007093 GO:0007094 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0008152 GO:0009506 GO:0009507 GO:0009536 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010564 GO:0010605 GO:0010639 GO:0010948 GO:0010965 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016973 GO:0019222 GO:0022402 GO:0022414 GO:0030054 GO:0030071 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031503 GO:0031577 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033233 GO:0033234 GO:0034613 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050793 GO:0050794 GO:0051028 GO:0051093 GO:0051128 GO:0051129 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051236 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071173 GO:0071174 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0090567 GO:0099402 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1903320 GO:1903321 GO:1905818 GO:1905819 GO:2000026 GO:2000241 GO:2000242 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko03013 ko04144 ko05200 ko05216 map03013 map04144 map05200 map05216
Pfam Domains
Protein Families
TPR_MLP1_2

Protein Analysis

2102

Amino Acids

236.78

Weight (kDa)

5.01

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoprot-TPR PF25481 170 - 249 5.1e-28 Nucleoprotein TPR-like domain
TPR PF25785 491 - 597 1.3e-30 Nucleoprotein TPR-like domain
TPR_MLP1_2 PF07926 1044 - 1171 1.2e-23 TPR/MLP1/MLP2-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 764, 2013, 5430
Acc16I TGCGCA 1 cut(s) 4589
Acc36I ACCTGC 2 cut(s) 164, 6217
Acc65I GGTACC 2 cut(s) 4745, 4922
AccB1I GGYRCC 2 cut(s) 4745, 4922
AccBSI CCGCTC 3 cut(s) 7, 2796, 4928
AccI GTMKAC 1 cut(s) 2528
AccII CGCG 3 cut(s) 224, 4074, 6267
AclI AACGTT 1 cut(s) 2247
AclWI GGATC 4 cut(s) 2793, 2806, 2901, 5605
AcoI YGGCCR 2 cut(s) 144, 4296
AcyI GRCGYC 6 cut(s) 48, 75, 129, 761, 2010, 5427
AflIII ACRYGT 1 cut(s) 1515
AhlI ACTAGT 2 cut(s) 1720, 2813
AjiI CACGTC 3 cut(s) 4178, 6239, 6254
AjnI CCWGG 6 cut(s) 1473, 2108, 3333, 4635, 5973, 6247
AjuI GAANNNNNNNTTGG 2 cut(s) 3200, 3232
AloI GAACNNNNNNTCC 2 cut(s) 1778, 1810
Alw21I GWGCWC 4 cut(s) 273, 780, 4864, 6139
AlwI GGATC 4 cut(s) 2793, 2806, 2901, 5605
AlwNI CAGNNNCTG 5 cut(s) 986, 3092, 4513, 5018, 5318
Ama87I CYCGRG 4 cut(s) 209, 2172, 4664, 5786
ApaI GGGCCC 1 cut(s) 121
ArsI GACNNNNNNTTYG 4 cut(s) 1502, 1534, 5043, 5075
Asp700I GAANNNNTTC 3 cut(s) 1902, 2307, 3672
Asp718I GGTACC 2 cut(s) 4745, 4922
AspLEI GCGC 7 cut(s) 226, 1890, 3747, 4076, 4590, 5030, 6267
AsuC2I CCSGG 2 cut(s) 4468, 5098
AsuHPI GGTGA 8 cut(s) 1567, 2842, 3596, 5138, 5741, 5861, 6091, 6177
AsuII TTCGAA 1 cut(s) 4824
AsuNHI GCTAGC 1 cut(s) 1061
AvaI CYCGRG 4 cut(s) 209, 2172, 4664, 5786
AvaII GGWCC 8 cut(s) 88, 885, 2434, 3569, 5154, 5701, 6206, 6242
AxyI CCTNAGG 2 cut(s) 4740, 5304
BaeGI GKGCMC 2 cut(s) 121, 3703
BalI TGGCCA 1 cut(s) 4298
BamHI GGATCC 1 cut(s) 2798
BanI GGYRCC 2 cut(s) 4745, 4922
BanII GRGCYC 5 cut(s) 121, 273, 780, 6139, 6177
BauI CACGAG 3 cut(s) 3053, 6132, 6276
BbsI GAAGAC 7 cut(s) 657, 1472, 2521, 3219, 4941, 6163, 6223
Bbv12I GWGCWC 4 cut(s) 273, 780, 4864, 6139
BbvCI CCTCAGC 3 cut(s) 1488, 2319, 4254
BceAI ACGGC 6 cut(s) 35, 55, 159, 1563, 1625, 2082
BciT130I CCWGG 6 cut(s) 1475, 2110, 3335, 4637, 5975, 6249
BciVI GTATCC 1 cut(s) 4987
BclI TGATCA 4 cut(s) 915, 1159, 1416, 1726
BcnI CCSGG 2 cut(s) 4468, 5098
BcuI ACTAGT 2 cut(s) 1720, 2813
BfuAI ACCTGC 2 cut(s) 164, 6217
BfuI GTATCC 1 cut(s) 4987
BglI GCCNNNNNGGC 1 cut(s) 3707
BglII AGATCT 5 cut(s) 1018, 2095, 2569, 5611, 6230
BlpI GCTNAGC 1 cut(s) 5002
Bme1390I CCNGG 8 cut(s) 1475, 2110, 3335, 4468, 4637, 5098, 5975, 6249
Bme18I GGWCC 8 cut(s) 88, 885, 2434, 3569, 5154, 5701, 6206, 6242
BmeT110I CYCGRG 4 cut(s) 209, 2172, 4664, 5786
BmgBI CACGTC 3 cut(s) 4178, 6239, 6254
BmrFI CCNGG 8 cut(s) 1475, 2110, 3335, 4468, 4637, 5098, 5975, 6249
BmrI ACTGGG 2 cut(s) 3718, 6054
BmtI GCTAGC 1 cut(s) 1065
BmuI ACTGGG 2 cut(s) 3718, 6054
BoxI GACNNNNGTC 1 cut(s) 6088
BpiI GAAGAC 7 cut(s) 657, 1472, 2521, 3219, 4941, 6163, 6223
BplI GAGNNNNNCTC 6 cut(s) 14, 46, 767, 799, 2905, 2937
Bpu10I CCTNAGC 6 cut(s) 1488, 1742, 2319, 2922, 3249, 4254
Bpu1102I GCTNAGC 1 cut(s) 5002
Bpu14I TTCGAA 1 cut(s) 4824
BpuMI CCSGG 2 cut(s) 4468, 5098
BsaAI YACGTR 1 cut(s) 521
BsaBI GATNNNNATC 3 cut(s) 3682, 4068, 4566
BsaHI GRCGYC 6 cut(s) 48, 75, 129, 761, 2010, 5427
BsaI GGTCTC 4 cut(s) 101, 894, 4337, 5673
BsaXI ACNNNNNCTCC 8 cut(s) 185, 215, 4355, 4385, 5645, 5675, 5685, 5715
Bse118I RCCGGY 1 cut(s) 4909
Bse21I CCTNAGG 2 cut(s) 4740, 5304
Bse3DI GCAATG 6 cut(s) 1926, 2834, 4590, 5062, 5167, 5212
Bse8I GATNNNNATC 3 cut(s) 3682, 4068, 4566
BseBI CCWGG 6 cut(s) 1475, 2110, 3335, 4637, 5975, 6249
BseJI GATNNNNATC 3 cut(s) 3682, 4068, 4566
BseMI GCAATG 6 cut(s) 1926, 2834, 4590, 5062, 5167, 5212
BseSI GKGCMC 2 cut(s) 121, 3703
BseX3I CGGCCG 1 cut(s) 144
BseYI CCCAGC 2 cut(s) 5501, 5797
BsgI GTGCAG 2 cut(s) 1757, 3476
Bsh1236I CGCG 3 cut(s) 224, 4074, 6267
Bsh1285I CGRYCG 1 cut(s) 147
BshNI GGYRCC 2 cut(s) 4745, 4922
BsiEI CGRYCG 1 cut(s) 147
BsiHKAI GWGCWC 4 cut(s) 273, 780, 4864, 6139
BsiHKCI CYCGRG 4 cut(s) 209, 2172, 4664, 5786
BsiSI CCGG 4 cut(s) 4467, 4910, 5098, 6213
BslFI GGGAC 8 cut(s) 101, 1157, 1612, 5167, 5251, 5371, 5687, 6042
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 8 cut(s) 101, 1157, 1612, 5167, 5251, 5371, 5687, 6042
BsmI GAATGC 3 cut(s) 1580, 3878, 6151
Bso31I GGTCTC 4 cut(s) 101, 894, 4337, 5673
BsoBI CYCGRG 4 cut(s) 209, 2172, 4664, 5786
Bsp119I TTCGAA 1 cut(s) 4824
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 7 cut(s) 121, 273, 780, 3703, 4864, 6139, 6177
Bsp1407I TGTACA 2 cut(s) 93, 1923
Bsp1720I GCTNAGC 1 cut(s) 5002
BspFNI CGCG 3 cut(s) 224, 4074, 6267
BspHI TCATGA 4 cut(s) 1288, 1612, 1621, 4653
BspMAI CTGCAG 6 cut(s) 805, 1018, 2448, 3097, 4205, 6073
BspMI ACCTGC 2 cut(s) 164, 6217
BspOI GCTAGC 1 cut(s) 1065
BspPI GGATC 4 cut(s) 2793, 2806, 2901, 5605
BspQI GCTCTTC 5 cut(s) 14, 807, 1872, 2574, 5511
BspT104I TTCGAA 1 cut(s) 4824
BspT107I GGYRCC 2 cut(s) 4745, 4922
BspTNI GGTCTC 4 cut(s) 101, 894, 4337, 5673
BsrBI CCGCTC 3 cut(s) 7, 2796, 4928
BsrDI GCAATG 6 cut(s) 1926, 2834, 4590, 5062, 5167, 5212
BsrFI RCCGGY 1 cut(s) 4909
BsrGI TGTACA 2 cut(s) 93, 1923
BssAI RCCGGY 1 cut(s) 4909
BssNAI GTATAC 1 cut(s) 2529
BssNI GRCGYC 6 cut(s) 48, 75, 129, 761, 2010, 5427
BssSI CACGAG 3 cut(s) 3053, 6132, 6276
BssT1I CCWWGG 4 cut(s) 525, 1175, 4374, 5287
Bst1107I GTATAC 1 cut(s) 2529
Bst2BI CACGAG 3 cut(s) 3053, 6132, 6276
Bst2UI CCWGG 6 cut(s) 1475, 2110, 3335, 4637, 5975, 6249
BstACI GRCGYC 6 cut(s) 48, 75, 129, 761, 2010, 5427
BstAPI GCANNNNNTGC 2 cut(s) 986, 2501
BstAUI TGTACA 2 cut(s) 93, 1923
BstBAI YACGTR 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 4824
BstDSI CCRYGG 2 cut(s) 37, 4938
BstENI CCTNNNNNAGG 4 cut(s) 95, 257, 1568, 5751
BstFNI CGCG 3 cut(s) 224, 4074, 6267
BstHHI GCGC 7 cut(s) 226, 1890, 3747, 4076, 4590, 5030, 6267
BstMCI CGRYCG 1 cut(s) 147
BstNI CCWGG 6 cut(s) 1475, 2110, 3335, 4637, 5975, 6249
BstNSI RCATGY 6 cut(s) 537, 644, 2390, 2687, 2902, 4724
BstPAI GACNNNNGTC 1 cut(s) 6088
BstSCI CCNGG 8 cut(s) 1473, 2108, 3333, 4466, 4635, 5096, 5973, 6247
BstSLI GKGCMC 2 cut(s) 121, 3703
BstUI CGCG 3 cut(s) 224, 4074, 6267
BstV2I GAAGAC 7 cut(s) 657, 1472, 2521, 3219, 4941, 6163, 6223
BstX2I RGATCY 6 cut(s) 1018, 2095, 2569, 2798, 5611, 6230
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 6 cut(s) 1018, 2095, 2569, 2798, 5611, 6230
BstZ17I GTATAC 1 cut(s) 2529
BstZI CGGCCG 1 cut(s) 144
Bsu36I CCTNAGG 2 cut(s) 4740, 5304
BsuI GTATCC 1 cut(s) 4987
BtgI CCRYGG 2 cut(s) 37, 4938
BtgZI GCGATG 5 cut(s) 172, 3297, 5604, 5750, 5823
BtrI CACGTC 3 cut(s) 4178, 6239, 6254
BtsI GCAGTG 1 cut(s) 1866
BveI ACCTGC 2 cut(s) 164, 6217
CaiI CAGNNNCTG 5 cut(s) 986, 3092, 4513, 5018, 5318
CciI TCATGA 4 cut(s) 1288, 1612, 1621, 4653
CfoI GCGC 7 cut(s) 226, 1890, 3747, 4076, 4590, 5030, 6267
Cfr10I RCCGGY 1 cut(s) 4909
CseI GACGC 3 cut(s) 56, 83, 137
DraI TTTAAA 2 cut(s) 717, 3144
EaeI YGGCCR 2 cut(s) 144, 4296
EagI CGGCCG 1 cut(s) 144
EciI GGCGGA 2 cut(s) 77, 356
Ecl136II GAGCTC 3 cut(s) 271, 778, 6137
EclXI CGGCCG 1 cut(s) 144
Eco130I CCWWGG 4 cut(s) 525, 1175, 4374, 5287
Eco24I GRGCYC 5 cut(s) 121, 273, 780, 6139, 6177
Eco31I GGTCTC 4 cut(s) 101, 894, 4337, 5673
Eco32I GATATC 3 cut(s) 1683, 3198, 5845
Eco47I GGWCC 8 cut(s) 88, 885, 2434, 3569, 5154, 5701, 6206, 6242
Eco52I CGGCCG 1 cut(s) 144
Eco53kI GAGCTC 3 cut(s) 271, 778, 6137
Eco81I CCTNAGG 2 cut(s) 4740, 5304
Eco88I CYCGRG 4 cut(s) 209, 2172, 4664, 5786
EcoICRI GAGCTC 3 cut(s) 271, 778, 6137
EcoNI CCTNNNNNAGG 4 cut(s) 95, 257, 1568, 5751
EcoO109I RGGNCCY 5 cut(s) 88, 117, 5094, 5154, 6206
EcoRI GAATTC 3 cut(s) 197, 1126, 3067
EcoRII CCWGG 6 cut(s) 1473, 2108, 3333, 4635, 5973, 6247
EcoRV GATATC 3 cut(s) 1683, 3198, 5845
EcoT14I CCWWGG 4 cut(s) 525, 1175, 4374, 5287
EcoT22I ATGCAT 1 cut(s) 2941
EcoT38I GRGCYC 5 cut(s) 121, 273, 780, 6139, 6177
ErhI CCWWGG 4 cut(s) 525, 1175, 4374, 5287
Esp3I CGTCTC 1 cut(s) 19
FaqI GGGAC 8 cut(s) 101, 1157, 1612, 5167, 5251, 5371, 5687, 6042
FauI CCCGC 3 cut(s) 41, 1218, 5165
FbaI TGATCA 4 cut(s) 915, 1159, 1416, 1726
FblI GTMKAC 1 cut(s) 2528
FriOI GRGCYC 5 cut(s) 121, 273, 780, 6139, 6177
FspI TGCGCA 1 cut(s) 4589
GlaI GCGC 7 cut(s) 225, 1889, 3746, 4075, 4589, 5029, 6266
GsaI CCCAGC 2 cut(s) 5505, 5801
HapII CCGG 4 cut(s) 4467, 4910, 5098, 6213
HgaI GACGC 3 cut(s) 56, 83, 137
HhaI GCGC 7 cut(s) 226, 1890, 3747, 4076, 4590, 5030, 6267
Hin1I GRCGYC 6 cut(s) 48, 75, 129, 761, 2010, 5427
Hin6I GCGC 7 cut(s) 224, 1888, 3745, 4074, 4588, 5028, 6265
HinP1I GCGC 7 cut(s) 224, 1888, 3745, 4074, 4588, 5028, 6265
HincII GTYRAC 9 cut(s) 600, 613, 2272, 2340, 3303, 3500, 4633, 6111, 6285
HindII GTYRAC 9 cut(s) 600, 613, 2272, 2340, 3303, 3500, 4633, 6111, 6285
HindIII AAGCTT 3 cut(s) 848, 2015, 3671
HpaI GTTAAC 2 cut(s) 600, 613
HpaII CCGG 4 cut(s) 4467, 4910, 5098, 6213
HphI GGTGA 8 cut(s) 1567, 2842, 3596, 5138, 5741, 5861, 6091, 6177
Hpy99I CGWCG 7 cut(s) 23, 50, 77, 131, 197, 3624, 5432
Hsp92I GRCGYC 6 cut(s) 48, 75, 129, 761, 2010, 5427
HspAI GCGC 7 cut(s) 224, 1888, 3745, 4074, 4588, 5028, 6265
KflI GGGWCCC 1 cut(s) 5154
KpnI GGTACC 2 cut(s) 4749, 4926
Ksp22I TGATCA 4 cut(s) 915, 1159, 1416, 1726
KspAI GTTAAC 2 cut(s) 600, 613
LguI GCTCTTC 5 cut(s) 14, 807, 1872, 2574, 5511
MbiI CCGCTC 3 cut(s) 7, 2796, 4928
MfeI CAATTG 2 cut(s) 4752, 5937
MflI RGATCY 6 cut(s) 1018, 2095, 2569, 2798, 5611, 6230
MhlI GDGCHC 7 cut(s) 121, 273, 780, 3703, 4864, 6139, 6177
MlsI TGGCCA 1 cut(s) 4298
MluNI TGGCCA 1 cut(s) 4298
MlyI GAGTC 6 cut(s) 221, 992, 1246, 4966, 5273, 5557
MmeI TCCRAC 7 cut(s) 42, 330, 2361, 2406, 3529, 3862, 3939
Mox20I TGGCCA 1 cut(s) 4298
Mph1103I ATGCAT 1 cut(s) 2941
MroXI GAANNNNTTC 3 cut(s) 1902, 2307, 3672
MscI TGGCCA 1 cut(s) 4298
MslI CAYNNNNRTG 5 cut(s) 645, 1506, 1626, 1833, 4943
Msp20I TGGCCA 1 cut(s) 4298
MspA1I CMGCKG 4 cut(s) 827, 3089, 4423, 5227
MspI CCGG 4 cut(s) 4467, 4910, 5098, 6213
MspR9I CCNGG 8 cut(s) 1475, 2110, 3335, 4468, 4637, 5098, 5975, 6249
MunI CAATTG 2 cut(s) 4752, 5937
Mva1269I GAATGC 3 cut(s) 1580, 3878, 6151
MvaI CCWGG 6 cut(s) 1475, 2110, 3335, 4637, 5975, 6249
MvnI CGCG 3 cut(s) 224, 4074, 6267
NciI CCSGG 2 cut(s) 4468, 5098
NheI GCTAGC 1 cut(s) 1061
NmeAIII GCCGAG 7 cut(s) 292, 1696, 2500, 2746, 3475, 3766, 5503
NmuCI GTSAC 6 cut(s) 1508, 1555, 2141, 2405, 2830, 3180
NsbI TGCGCA 1 cut(s) 4589
NsiI ATGCAT 1 cut(s) 2941
NspI RCATGY 6 cut(s) 537, 644, 2390, 2687, 2902, 4724
NspV TTCGAA 1 cut(s) 4824
PaeI GCATGC 2 cut(s) 644, 2687
PaeR7I CTCGAG 3 cut(s) 209, 2172, 4664
PagI TCATGA 4 cut(s) 1288, 1612, 1621, 4653
PciSI GCTCTTC 5 cut(s) 14, 807, 1872, 2574, 5511
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 2287, 4183, 4821
PctI GAATGC 3 cut(s) 1580, 3878, 6151
PdmI GAANNNNTTC 3 cut(s) 1902, 2307, 3672
PflFI GACNNNGTC 2 cut(s) 50, 4369
PfoI TCCNGGA 3 cut(s) 1473, 3333, 6247
PleI GAGTC 6 cut(s) 220, 991, 1245, 4965, 5272, 5556
PpsI GAGTC 6 cut(s) 220, 991, 1245, 4965, 5272, 5556
Ppu21I YACGTR 1 cut(s) 521
PpuMI RGGWCCY 3 cut(s) 88, 5154, 6206
PshAI GACNNNNGTC 1 cut(s) 6088
Psp124BI GAGCTC 3 cut(s) 273, 780, 6139
Psp1406I AACGTT 1 cut(s) 2247
Psp5II RGGWCCY 3 cut(s) 88, 5154, 6206
Psp6I CCWGG 6 cut(s) 1473, 2108, 3333, 4635, 5973, 6247
PspFI CCCAGC 2 cut(s) 5501, 5797
PspGI CCWGG 6 cut(s) 1473, 2108, 3333, 4635, 5973, 6247
PspOMI GGGCCC 1 cut(s) 117
PspPPI RGGWCCY 3 cut(s) 88, 5154, 6206
PspXI VCTCGAGB 1 cut(s) 209
PstI CTGCAG 6 cut(s) 805, 1018, 2448, 3097, 4205, 6073
PstNI CAGNNNCTG 5 cut(s) 986, 3092, 4513, 5018, 5318
PsuI RGATCY 6 cut(s) 1018, 2095, 2569, 2798, 5611, 6230
PsyI GACNNNGTC 2 cut(s) 50, 4369
PvuII CAGCTG 2 cut(s) 4423, 5227
RseI CAYNNNNRTG 5 cut(s) 645, 1506, 1626, 1833, 4943
SacI GAGCTC 3 cut(s) 273, 780, 6139
SapI GCTCTTC 5 cut(s) 14, 807, 1872, 2574, 5511
SchI GAGTC 6 cut(s) 221, 992, 1246, 4966, 5273, 5557
ScrFI CCNGG 8 cut(s) 1475, 2110, 3335, 4468, 4637, 5098, 5975, 6249
SduI GDGCHC 7 cut(s) 121, 273, 780, 3703, 4864, 6139, 6177
Sfr274I CTCGAG 3 cut(s) 209, 2172, 4664
SfuI TTCGAA 1 cut(s) 4824
SinI GGWCC 8 cut(s) 88, 885, 2434, 3569, 5154, 5701, 6206, 6242
SlaI CTCGAG 3 cut(s) 209, 2172, 4664
SmiMI CAYNNNNRTG 5 cut(s) 645, 1506, 1626, 1833, 4943
SpeI ACTAGT 2 cut(s) 1720, 2813
SphI GCATGC 2 cut(s) 644, 2687
SspI AATATT 2 cut(s) 3426, 4051
SstI GAGCTC 3 cut(s) 273, 780, 6139
StyD4I CCNGG 8 cut(s) 1473, 2108, 3333, 4466, 4635, 5096, 5973, 6247
StyI CCWWGG 4 cut(s) 525, 1175, 4374, 5287
TaqII GACCGA 1 cut(s) 5697
TatI WGTACW 4 cut(s) 93, 536, 1923, 3459
TauI GCSGC 6 cut(s) 7, 134, 252, 1859, 3092, 5446
TseFI GTSAC 6 cut(s) 1508, 1555, 2141, 2405, 2830, 3180
Tsp45I GTSAC 6 cut(s) 1508, 1555, 2141, 2405, 2830, 3180
TspGWI ACGGA 5 cut(s) 2614, 3637, 3875, 5978, 6096
Tth111I GACNNNGTC 2 cut(s) 50, 4369
VpaK11BI GGWCC 8 cut(s) 88, 885, 2434, 3569, 5154, 5701, 6206, 6242
XagI CCTNNNNNAGG 4 cut(s) 95, 257, 1568, 5751
XbaI TCTAGA 2 cut(s) 1847, 4415
XceI RCATGY 6 cut(s) 537, 644, 2390, 2687, 2902, 4724
XhoI CTCGAG 3 cut(s) 209, 2172, 4664
XmiI GTMKAC 1 cut(s) 2528
XmnI GAANNNNTTC 3 cut(s) 1902, 2307, 3672
ZraI GACGTC 3 cut(s) 762, 2011, 5428
Zsp2I ATGCAT 1 cut(s) 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.