pycom05g21680

Nuclear-pore

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003251
Physical Location & Seq
Forward (+)
24240972 .. 24241520
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21680.1

Sequence Viewer

Length: 549 bp
ATGGGCAGTCCTGAAATAGGGGAAGTTCAACCTGAACCTGTAACAACTCCCGAGGCTTCCCCAGCTAGGGGGGATGATTATGGTGTTGCTGCTGGTTCTGTTGTGGATATCGGTGAGGTTAATTCTCCAGAGGTTCTTAATGATGAGAAAAATGATGACATTGATGCAACTGAAGAAGCTGCTGATGGTTCCGATAAGTCAATTGATGGTAATGACCATACTGTGACGGAGACTGACCAGGCTGCCGAAGCTACCTCAGTAATCGTAGATACCACTTCAACAAGTACTACAGCAGAAGTAAGCGTTTCAAAACAAGCTAGTCCCAGTGTTACTGCAGAAGAGGTGAGACAGGTGTCTCCTGTGACTATTCCGTCAACTACCATAAATTTAACAACACGAGCTCGGGAGAATGCACGCCGAAGACAACAGGCCAGAGGGCTTTCTACTACACCAAGCACAGGTCGTGGAGGACCTGTCGGAAGACAAACCGGAAGATCTGGACGTGGACCTCCTGGACGTGGGCGTGGGCGCGGACATCCTCGTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000278 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005829 GO:0005911 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007088 GO:0007093 GO:0007094 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0008152 GO:0009506 GO:0009507 GO:0009536 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010564 GO:0010605 GO:0010639 GO:0010948 GO:0010965 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016973 GO:0019222 GO:0022402 GO:0022414 GO:0030054 GO:0030071 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031503 GO:0031577 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033233 GO:0033234 GO:0034613 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050793 GO:0050794 GO:0051028 GO:0051093 GO:0051128 GO:0051129 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051236 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071173 GO:0071174 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0090567 GO:0099402 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1903320 GO:1903321 GO:1905818 GO:1905819 GO:2000026 GO:2000241 GO:2000242 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko03013 ko04144 ko05200 ko05216 map03013 map04144 map05200 map05216
Pfam Domains
Protein Families
TPR_MLP1_2

Protein Analysis

183

Amino Acids

18.79

Weight (kDa)

4.76

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 370
AccII CGCG 1 cut(s) 531
AciI CCGC 1 cut(s) 531
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 1 cut(s) 286
AfiI CCNNNNNNNGG 6 cut(s) 17, 66, 67, 68, 458, 518
AgsI TTSAA 3 cut(s) 29, 279, 309
AjiI CACGTC 2 cut(s) 503, 518
AjnI CCWGG 2 cut(s) 237, 511
AluBI AGCT 5 cut(s) 65, 179, 251, 317, 401
AluI AGCT 5 cut(s) 65, 179, 251, 317, 401
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 3 cut(s) 224, 340, 360
Ama87I CYCGRG 2 cut(s) 50, 402
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 89, 179, 242
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 2 cut(s) 470, 506
AsuHPI GGTGA 2 cut(s) 125, 355
AvaI CYCGRG 2 cut(s) 50, 402
AvaII GGWCC 2 cut(s) 470, 506
BanII GRGCYC 1 cut(s) 403
BauI CACGAG 2 cut(s) 396, 540
BbsI GAAGAC 2 cut(s) 427, 487
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 3 cut(s) 76, 166, 229
BccI CCATC 2 cut(s) 179, 200
BciT130I CCWGG 2 cut(s) 239, 513
BcoDI GTCTC 3 cut(s) 224, 340, 360
BfaI CTAG 2 cut(s) 66, 318
BfmI CTRYAG 2 cut(s) 288, 333
BglII AGATCT 1 cut(s) 494
BisI GCNGC 3 cut(s) 90, 180, 243
BlsI GCNGC 3 cut(s) 91, 181, 244
BmcAI AGTACT 1 cut(s) 286
Bme1390I CCNGG 2 cut(s) 239, 513
Bme18I GGWCC 2 cut(s) 470, 506
BmeT110I CYCGRG 2 cut(s) 50, 402
BmgBI CACGTC 2 cut(s) 503, 518
BmgT120I GGNCC 2 cut(s) 470, 506
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 239, 513
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 318
BoxI GACNNNNGTC 1 cut(s) 352
BpiI GAAGAC 2 cut(s) 427, 487
BpmI CTGGAG 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 488
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 66, 67, 68, 458, 518
Bse1I ACTGG 1 cut(s) 324
BseBI CCWGG 2 cut(s) 239, 513
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 2 cut(s) 79, 535
BseLI CCNNNNNNNGG 6 cut(s) 17, 66, 67, 68, 458, 518
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 324
BseXI GCAGC 3 cut(s) 76, 166, 229
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 431
BsiHKAI GWGCWC 1 cut(s) 403
BsiHKCI CYCGRG 2 cut(s) 50, 402
BsiSI CCGG 1 cut(s) 489
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 6 cut(s) 17, 66, 67, 68, 458, 518
BsmAI GTCTC 3 cut(s) 224, 340, 360
BsmFI GGGAC 1 cut(s) 306
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 1 cut(s) 431
BsoBI CYCGRG 2 cut(s) 50, 402
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 1 cut(s) 494
BspACI CCGC 1 cut(s) 531
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 1 cut(s) 269
BspFNI CGCG 1 cut(s) 531
BspLI GGNNCC 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 324
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 494
BssSI CACGAG 2 cut(s) 396, 540
Bst2BI CACGAG 2 cut(s) 396, 540
Bst2UI CCWGG 2 cut(s) 239, 513
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 1 cut(s) 256
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 79, 535
BstFNI CGCG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 1 cut(s) 497
BstMAI GTCTC 3 cut(s) 224, 340, 360
BstMBI GATC 1 cut(s) 494
BstMWI GCNNNNNNNGC 2 cut(s) 62, 248
BstNI CCWGG 2 cut(s) 239, 513
BstPAI GACNNNNGTC 1 cut(s) 352
BstSCI CCNGG 2 cut(s) 237, 511
BstSFI CTRYAG 2 cut(s) 288, 333
BstUI CGCG 1 cut(s) 531
BstV1I GCAGC 3 cut(s) 76, 166, 229
BstV2I GAAGAC 2 cut(s) 427, 487
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
BsuRI GGCC 1 cut(s) 431
BtrI CACGTC 2 cut(s) 503, 518
BtsCI GGATG 2 cut(s) 79, 535
BtsIMutI CAGTG 1 cut(s) 331
Cac8I GCNNGC 1 cut(s) 415
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 470, 506
Csp6I GTAC 1 cut(s) 285
CviJI RGCY 9 cut(s) 56, 65, 179, 242, 251, 317, 401, 431, 439
CviKI_1 RGCY 9 cut(s) 56, 65, 179, 242, 251, 317, 401, 431, 439
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 1 cut(s) 256
DpnI GATC 1 cut(s) 496
DpnII GATC 1 cut(s) 494
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 403
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 470, 506
Eco53kI GAGCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 192
Eco88I CYCGRG 2 cut(s) 50, 402
EcoICRI GAGCTC 1 cut(s) 401
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 470
EcoRII CCWGG 2 cut(s) 237, 511
EcoRV GATATC 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 403
FaiI YATR 3 cut(s) 81, 219, 383
FaqI GGGAC 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 90, 180, 243
FokI GGATG 2 cut(s) 86, 522
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 3 cut(s) 90, 180, 243
FspBI CTAG 2 cut(s) 66, 318
GlaI GCGC 1 cut(s) 530
GluI GCNGC 3 cut(s) 90, 180, 243
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 1 cut(s) 431
HapII CCGG 1 cut(s) 489
HhaI GCGC 1 cut(s) 531
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HpaII CCGG 1 cut(s) 489
HphI GGTGA 2 cut(s) 125, 355
Hpy166II GTNNAC 2 cut(s) 375, 506
Hpy188I TCNGA 2 cut(s) 193, 479
Hpy188III TCNNGA 5 cut(s) 11, 50, 128, 404, 498
Hpy8I GTNNAC 2 cut(s) 375, 506
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 2 cut(s) 502, 517
HpyCH4V TGCA 3 cut(s) 167, 335, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 248
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 2 cut(s) 502, 517
HspAI GCGC 1 cut(s) 529
Kzo9I GATC 1 cut(s) 494
Lsp1109I GCAGC 3 cut(s) 76, 166, 229
LweI GCATC 1 cut(s) 154
MaeI CTAG 2 cut(s) 66, 318
MaeII ACGT 2 cut(s) 502, 517
MaeIII GTNAC 4 cut(s) 40, 223, 328, 361
MalI GATC 1 cut(s) 496
MboI GATC 1 cut(s) 494
MboII GAAGA 5 cut(s) 185, 350, 432, 492, 504
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 1 cut(s) 494
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 3 cut(s) 121, 201, 385
MmeI TCCRAC 1 cut(s) 457
MnlI CCTC 9 cut(s) 46, 109, 124, 265, 334, 428, 461, 519, 549
MseI TTAA 3 cut(s) 120, 138, 389
MslI CAYNNNNRTG 1 cut(s) 540
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 2 cut(s) 239, 513
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 2 cut(s) 239, 513
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 62, 248
NdeII GATC 1 cut(s) 494
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 2 cut(s) 223, 361
PctI GAATGC 1 cut(s) 415
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 3 cut(s) 91, 181, 244
PpuMI RGGWCCY 1 cut(s) 470
PshAI GACNNNNGTC 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 403
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 2 cut(s) 237, 511
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 2 cut(s) 237, 511
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 2 cut(s) 470, 506
PspPPI RGGWCCY 1 cut(s) 470
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 494
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
RseI CAYNNNNRTG 1 cut(s) 540
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 3 cut(s) 120, 138, 389
SatI GCNGC 3 cut(s) 90, 180, 243
Sau3AI GATC 1 cut(s) 494
Sau96I GGNCC 2 cut(s) 470, 506
ScaI AGTACT 1 cut(s) 286
ScrFI CCNGG 2 cut(s) 239, 513
SduI GDGCHC 1 cut(s) 403
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 2 cut(s) 288, 333
SinI GGWCC 2 cut(s) 470, 506
SmiMI CAYNNNNRTG 1 cut(s) 540
Sse9I AATT 3 cut(s) 121, 201, 385
SsiI CCGC 1 cut(s) 531
SspMI CTAG 2 cut(s) 66, 318
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 2 cut(s) 237, 511
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 2 cut(s) 505, 520
TasI AATT 3 cut(s) 121, 201, 385
TatI WGTACW 1 cut(s) 284
Tru1I TTAA 3 cut(s) 120, 138, 389
Tru9I TTAA 3 cut(s) 120, 138, 389
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 2 cut(s) 223, 361
TseI GCWGC 3 cut(s) 89, 179, 242
Tsp45I GTSAC 2 cut(s) 223, 361
TspGWI ACGGA 2 cut(s) 242, 360
TspRI CASTG 1 cut(s) 331
VpaK11BI GGWCC 2 cut(s) 470, 506
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 385
XspI CTAG 2 cut(s) 66, 318
ZrmI AGTACT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.