MD10G1219000.v1.1

Nuclear-pore

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003251
Physical Location & Seq
Forward (+)
31667798 .. 31668481
684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1219000.v1.1.491

Sequence Viewer

Length: 684 bp
ATGCCATCACACGATGGAGCCAAAGATCAGGTTGAGCTGGAGCAACAGTCCAGTGATTTTGGAGGAGACCGAGAAGAGGGAGAGCTGGTCCCTGATATTTCTGAGCTTGAAGGTGGTGGTGATACGATGGCCAGTCCTGAAATAGGGGAAGTTCAACCTGAACCTGTAACAACTCCCGAGGCTTCCCCAGCTAGGGGCGATGATTATGGTGTTGCTGCTGGTTCTGTTGTGGATATCAGTGAGGTTAATTATCCAGAGATTCTTAATGATGACATTGATGCGACTGAAGAAACCGCTGATGGTTCCGATAAGTCAATTGACGGTAATGACCAAACTGTGATGGAGACTGATCAGGCTGCCGAAGCTACCTCAGTAATCGTGGATACCACTTCAACAGGTACCACATCGGAAGTTAGCGTTTCAAAACAAACTAGTCCCAGTCTTGCGGCAGAAGAGGTGAGACAGGTGTCTCCAGTGACTAATCCGTCAACTACCATAAATATAACAGAACGAGCCGTGGCGAATGCACGCCGAAGACAACAGGCAAGAGGGCTTTCTACATCACCAAGCCCAGGTCGTGGTGGACCAGCTGGAAGACAAGGTGGGAGACCTGGACGTGCCCCTCCTGGACGCGGACGCGGACGTGGACGATCTCGTGGGTCAACATCTGGCGATCAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000278 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0005829 GO:0005911 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0007049 GO:0007088 GO:0007093 GO:0007094 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0008152 GO:0009506 GO:0009507 GO:0009536 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009910 GO:0009987 GO:0010467 GO:0010468 GO:0010564 GO:0010605 GO:0010639 GO:0010948 GO:0010965 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016973 GO:0019222 GO:0022402 GO:0022414 GO:0030054 GO:0030071 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031503 GO:0031577 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033233 GO:0033234 GO:0034613 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0046907 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048519 GO:0048523 GO:0048580 GO:0048581 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050793 GO:0050794 GO:0051028 GO:0051093 GO:0051128 GO:0051129 GO:0051168 GO:0051169 GO:0051171 GO:0051172 GO:0051179 GO:0051234 GO:0051236 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051641 GO:0051649 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0055044 GO:0060255 GO:0060968 GO:0061458 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071173 GO:0071174 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0090567 GO:0099402 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1903320 GO:1903321 GO:1905818 GO:1905819 GO:2000026 GO:2000241 GO:2000242 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko03013 ko04144 ko05200 ko05216 map03013 map04144 map05200 map05216
Pfam Domains
Protein Families
TPR_MLP1_2

Protein Analysis

228

Amino Acids

23.42

Weight (kDa)

4.31

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 484
Acc65I GGTACC 1 cut(s) 398
AccB1I GGYRCC 1 cut(s) 398
AccB7I CCANNNNNTGG 1 cut(s) 578
AccII CGCG 2 cut(s) 633, 639
AciI CCGC 4 cut(s) 294, 446, 633, 639
AcoI YGGCCR 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 8 cut(s) 76, 143, 192, 193, 194, 572, 578, 632
AgsI TTSAA 4 cut(s) 110, 155, 393, 423
AhlI ACTAGT 1 cut(s) 431
AjiI CACGTC 2 cut(s) 617, 644
AjnI CCWGG 3 cut(s) 571, 610, 625
AluBI AGCT 6 cut(s) 37, 85, 106, 191, 365, 590
AluI AGCT 6 cut(s) 37, 85, 106, 191, 365, 590
Alw26I GTCTC 5 cut(s) 60, 338, 454, 474, 601
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 215, 356
Asp718I GGTACC 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 88, 584
AsuHPI GGTGA 3 cut(s) 131, 469, 555
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 2 cut(s) 88, 584
BaeGI GKGCMC 1 cut(s) 622
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 1 cut(s) 398
BauI CACGAG 1 cut(s) 654
BbsI GAAGAC 2 cut(s) 541, 601
BbvI GCAGC 2 cut(s) 202, 343
BccI CCATC 5 cut(s) 8, 13, 121, 293, 334
BceAI ACGGC 1 cut(s) 500
BciT130I CCWGG 3 cut(s) 573, 612, 627
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 5 cut(s) 60, 338, 454, 474, 601
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 2 cut(s) 192, 432
BfuI GTATCC 1 cut(s) 376
BisI GCNGC 3 cut(s) 216, 357, 447
BlsI GCNGC 3 cut(s) 217, 358, 448
Bme1390I CCNGG 3 cut(s) 573, 612, 627
Bme18I GGWCC 2 cut(s) 88, 584
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 2 cut(s) 617, 644
BmgT120I GGNCC 2 cut(s) 88, 584
BmiI GGNNCC 4 cut(s) 19, 90, 304, 400
BmrFI CCNGG 3 cut(s) 573, 612, 627
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 1 cut(s) 268
BmuI ACTGGG 1 cut(s) 432
BoxI GACNNNNGTC 1 cut(s) 466
BpiI GAAGAC 2 cut(s) 541, 601
BpmI CTGGAG 2 cut(s) 59, 456
BsaI GGTCTC 2 cut(s) 60, 601
BsaJI CCNNGG 3 cut(s) 177, 516, 571
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 72, 102
Bsc4I CCNNNNNNNGG 8 cut(s) 76, 143, 192, 193, 194, 572, 578, 632
Bse1I ACTGG 4 cut(s) 51, 132, 438, 473
BseBI CCWGG 3 cut(s) 573, 612, 627
BseDI CCNNGG 3 cut(s) 177, 516, 571
BseLI CCNNNNNNNGG 8 cut(s) 76, 143, 192, 193, 194, 572, 578, 632
BseMII CTCAG 2 cut(s) 93, 384
BseNI ACTGG 4 cut(s) 51, 132, 438, 473
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 622
BseXI GCAGC 2 cut(s) 202, 343
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 2 cut(s) 633, 639
BshFI GGCC 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 398
BsiHKCI CYCGRG 1 cut(s) 176
BslFI GGGAC 2 cut(s) 74, 420
BslI CCNNNNNNNGG 8 cut(s) 76, 143, 192, 193, 194, 572, 578, 632
BsmAI GTCTC 5 cut(s) 60, 338, 454, 474, 601
BsmFI GGGAC 2 cut(s) 74, 420
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 1 cut(s) 131
Bso31I GGTCTC 2 cut(s) 60, 601
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 4 cut(s) 25, 349, 650, 673
BspACI CCGC 4 cut(s) 294, 446, 633, 639
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 2 cut(s) 94, 383
BspFNI CGCG 2 cut(s) 633, 639
BspLI GGNNCC 4 cut(s) 19, 90, 304, 400
BspT107I GGYRCC 1 cut(s) 398
BspTNI GGTCTC 2 cut(s) 60, 601
BsrI ACTGG 4 cut(s) 51, 132, 438, 473
BssECI CCNNGG 3 cut(s) 177, 516, 571
BssMI GATC 4 cut(s) 25, 349, 650, 673
BssSI CACGAG 1 cut(s) 654
Bst2BI CACGAG 1 cut(s) 654
Bst2UI CCWGG 3 cut(s) 573, 612, 627
Bst4CI ACNGT 3 cut(s) 48, 323, 337
Bst6I CTCTTC 2 cut(s) 69, 447
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 2 cut(s) 102, 370
BstDSI CCRYGG 1 cut(s) 516
BstENI CCTNNNNNAGG 1 cut(s) 141
BstFNI CGCG 2 cut(s) 633, 639
BstKTI GATC 4 cut(s) 28, 352, 653, 676
BstMAI GTCTC 5 cut(s) 60, 338, 454, 474, 601
BstMBI GATC 4 cut(s) 25, 349, 650, 673
BstMWI GCNNNNNNNGC 2 cut(s) 188, 362
BstNI CCWGG 3 cut(s) 573, 612, 627
BstPAI GACNNNNGTC 1 cut(s) 466
BstSCI CCNGG 3 cut(s) 571, 610, 625
BstSLI GKGCMC 1 cut(s) 622
BstUI CGCG 2 cut(s) 633, 639
BstV1I GCAGC 2 cut(s) 202, 343
BstV2I GAAGAC 2 cut(s) 541, 601
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 213
BtrI CACGTC 2 cut(s) 617, 644
BtsIMutI CAGTG 3 cut(s) 58, 244, 480
Cac8I GCNNGC 1 cut(s) 529
Cfr13I GGNCC 2 cut(s) 88, 584
CseI GACGC 2 cut(s) 639, 645
Csp6I GTAC 1 cut(s) 399
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 2 cut(s) 102, 370
DpnI GATC 4 cut(s) 27, 351, 652, 675
DpnII GATC 4 cut(s) 25, 349, 650, 673
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 69, 447
EarI CTCTTC 2 cut(s) 69, 447
Eco31I GGTCTC 2 cut(s) 60, 601
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 2 cut(s) 88, 584
Eco57I CTGAAG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 176
EcoNI CCTNNNNNAGG 1 cut(s) 141
EcoRII CCWGG 3 cut(s) 571, 610, 625
EcoRV GATATC 1 cut(s) 235
FaiI YATR 3 cut(s) 207, 497, 503
FaqI GGGAC 2 cut(s) 74, 420
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 3 cut(s) 216, 357, 447
Fsp4HI GCNGC 3 cut(s) 216, 357, 447
FspBI CTAG 2 cut(s) 192, 432
GluI GCNGC 3 cut(s) 216, 357, 447
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 59, 456
HaeIII GGCC 1 cut(s) 131
HgaI GACGC 2 cut(s) 639, 645
HincII GTYRAC 2 cut(s) 489, 663
HindII GTYRAC 2 cut(s) 489, 663
HinfI GANTC 1 cut(s) 259
HphI GGTGA 3 cut(s) 131, 469, 555
Hpy166II GTNNAC 4 cut(s) 489, 584, 647, 663
Hpy188I TCNGA 3 cut(s) 103, 307, 409
Hpy188III TCNNGA 3 cut(s) 137, 176, 254
Hpy8I GTNNAC 4 cut(s) 489, 584, 647, 663
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 3 cut(s) 48, 323, 337
HpyCH4IV ACGT 2 cut(s) 616, 643
HpyCH4V TGCA 1 cut(s) 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 362
HpyF3I CTNAG 2 cut(s) 102, 370
HpySE526I ACGT 2 cut(s) 616, 643
KpnI GGTACC 1 cut(s) 402
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 4 cut(s) 25, 349, 650, 673
LmnI GCTCC 2 cut(s) 17, 40
Lsp1109I GCAGC 2 cut(s) 202, 343
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 192, 432
MaeII ACGT 2 cut(s) 616, 643
MaeIII GTNAC 2 cut(s) 166, 475
MalI GATC 4 cut(s) 27, 351, 652, 675
MboI GATC 4 cut(s) 25, 349, 650, 673
MboII GAAGA 5 cut(s) 86, 299, 464, 546, 606
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 622
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 2 cut(s) 247, 315
MluNI TGGCCA 1 cut(s) 131
MnlI CCTC 8 cut(s) 56, 70, 172, 235, 379, 448, 542, 633
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 2 cut(s) 246, 264
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 2 cut(s) 296, 590
MspR9I CCNGG 3 cut(s) 573, 612, 627
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 3 cut(s) 573, 612, 627
MvnI CGCG 2 cut(s) 633, 639
MwoI GCNNNNNNNGC 2 cut(s) 188, 362
NdeII GATC 4 cut(s) 25, 349, 650, 673
NlaIV GGNNCC 4 cut(s) 19, 90, 304, 400
NmuCI GTSAC 1 cut(s) 475
PctI GAATGC 1 cut(s) 529
PfeI GAWTC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 625
PkrI GCNGC 3 cut(s) 217, 358, 448
PshAI GACNNNNGTC 1 cut(s) 466
Psp6I CCWGG 3 cut(s) 571, 610, 625
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 3 cut(s) 571, 610, 625
PspN4I GGNNCC 4 cut(s) 19, 90, 304, 400
PspPI GGNCC 2 cut(s) 88, 584
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
SaqAI TTAA 2 cut(s) 246, 264
SatI GCNGC 3 cut(s) 216, 357, 447
Sau3AI GATC 4 cut(s) 25, 349, 650, 673
Sau96I GGNCC 2 cut(s) 88, 584
ScrFI CCNGG 3 cut(s) 573, 612, 627
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 1 cut(s) 268
SinI GGWCC 2 cut(s) 88, 584
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 2 cut(s) 247, 315
SsiI CCGC 4 cut(s) 294, 446, 633, 639
SspMI CTAG 2 cut(s) 192, 432
StyD4I CCNGG 3 cut(s) 571, 610, 625
TaaI ACNGT 3 cut(s) 48, 323, 337
TaiI ACGT 2 cut(s) 619, 646
TaqII GACCGA 1 cut(s) 84
TasI AATT 2 cut(s) 247, 315
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 246, 264
Tru9I TTAA 2 cut(s) 246, 264
TscAI CASTG 3 cut(s) 58, 244, 480
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 215, 356
Tsp45I GTSAC 1 cut(s) 475
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 3 cut(s) 58, 244, 480
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 2 cut(s) 88, 584
XagI CCTNNNNNAGG 1 cut(s) 141
XspI CTAG 2 cut(s) 192, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.