Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006269

Physical Location & Seq

Genomic Coordinates Reverse (-)

46880010 .. 46883050

Gene Structure 3041 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1352200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGATCTTAATTATCTATGGTTTGATGCTTATGGATATGGTTTGTGAAGTTTTCAAGGCCCATGCAAAGCGATTGGTGGATTCTCATGACCAACTTCTACAAAGTGCTTTAGAGACTGGAAGCTACAGCAAGCTTTACAGCTTTGAACACATTGTAAATGGCTTTGCTGTCCATACCATCCCTTCCCAGGCTGAGAAGCTCAAAGATGCGGCAGGAGTGAAGTTGGTTGAGAGAGACAGAGGAGCAAGGTTGATGGCAACGTACTGTCCTGAATTCCTTGGACTACCCCAAGGAGCATGGACACTGGAGGGAGGAGATGATCGTGGCACCGGCGAAGGAATTGTGATTGGTTTTGTGGATTCAGGCATCAATCCCACACACCCCAGTTTTGCTTATGATCCCCTGCACCGTTTCACCTCAAACATTTCTCATTTTTCAGACGCTTGCGAAACGGGTCCTCGATTCCCAGCCAGTTCATGCAATGGGAAAATAGTCTCGGCAAAGTTCTTCTCAGCTGGGGCTGAAGCTGCTGCCACTCTTAACTCTACAGTGGATTTTCTTTCACCCTTTTATGCAGTTGGTCATGGAAGTCACGTGGCATCAACGGCTGCGGGTAATGCTGGAGTTCCAGTGGTTGTAAATGGTTACTACTATGGAGAAGCAAGTGGGATGGCTCCACGGGCGCGAATTGCTGTATATAAAGCTGTGTATCCTACAGTGGGAACTTTAACAGATGTAGTTTCAGCAATTGATCACGCAATTCTAGATGGAGTGGACATCCTAACACTTTCTGTAGGACCAGATGAACCTCCAGAAGATACTGTTACCTTCTTAAGCACGTATGATATTGCCATGTTATCTGCTCGAAGAGCTGGGGTTTTTATGTTGCAAGCAGCAGGCAATCAAGGGCCAGGTCCTTCGAGCGTAGTCTCTTACAGTCCTTGGGCTGTAGCTGTCGCTTCATCTGGCACTGATAGAAATTACCCCAGTTCTATTCTTCTCGGAAATGGCACAAAAATCGAAGGACCGACTTTTGGATATGGTTTACTTTTGCATAAGTTGGATTTGGCAAGGGATGCAGTGAAGGCAGATGGGACGTTCCCAAGGACTCCGCCTTATGTCGAGGAGTGCCAATATCCTGAAGCATTGGATCACAATGTAGTGCAAGGTAGCATCGTCATTTGCACCTTCTCAGCGGGATTCTACAACGGAACATCCAGCCTCACTGCCATCATCAACACGGCAAAAGGTCTAGGGTTTATGGGTTTTATCCTTGTTGCAAATCCAGCCTGCGGTGATTTCATTGCAGAACCTATTCCTTTTGTTGTTCCTGGCATTTTGGTCCCCAATTTAACCAATTCCCAGGTTATATTACAATATTATGAACAAGAAACACACGAGGAGAAAGGATTTGATACTCGATTTGCAGCAAAAGCAGCTATTGGAGAAGGAAGAGTTGCTTCTTTCATGGGACAAGCGCCCGTTGTAAGCAGATTCTCTTCCAGGGGACCAGATTTCACAAACACCTCCAGGACTCCTTGTGATGTACTAAAGCCGGATATTCTTGCCCCAGGGCACCAAATTTGGGCAGCTTGGAGTCCCTTTAGCGCCTCAGAGCCCGCTTTAACAGGCTACAATTTTGCACCACAATCTGGTACAAGCATGGCAACACCTCAAGTAGTGGGAATAGCAGCACTCATCAAGCAGTACAATCCTTCATGGATTCCATCCATGATCGCGTCTGCAATTTCCACCACTGCCACAAAGTATGACAACAACGGAGAACTTGAAATGGCAGAAGGATCCGGCATAGGCAGTACATATCCGTCCAGCCATTTTGATTTAGGAGCTGGATTCGTCAGTCCAAGTCGAGCCATGGATCCCGGCCTAGTCTTACCTTCTGGATATGAAGATTATATCAGCTTCTTGTGCTCATTGCCTGAAATCAAAAGTCCAGCTACAATTAGAAATGCAACCGGACAACTGCGCAACTACACACTTGACCATCCGGCCAATTTGAACCTTCCTTCGATAACAATATCGGCATTGAGGGGTCTCAAATCATCCGACGGATTTTCAATAACGTGGGGAGCAAGCCGGAGACTTTTGTTTCCTCTGCGGTGTCACCTAATGGGACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0032501 GO:0032502 GO:0042335 GO:0043170 GO:0044238 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

714

Amino Acids

75.78

Weight (kDa)

5.45

Isoelectric Point (pI)

36.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	26 - 84	2.3e-09	Peptidase inhibitor I9
Peptidase_S8	PF00082	111 - 597	7.4e-38	Subtilase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006269)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62340
fragaria_vesca	FvH4_3g01420
malus_domestica	MD05G1352200.v1.1 MD10G1326200.v1.1
prunus_persica	Prupe.4G015500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002151 RchiOBHm_Chr5g0002191
rosa_laevigata	RLG00000031001 RLG00000031002 RLG00000031007
rosa_multiflora	Rmu_co8154878.1_g000001 Rmu_sc0001492.1_g000001 Rmu_sc0007900.1_g000001 Rmu_sc0013155.1_g000003 Rmu_ssc0000001.1_g000005
rosa_roxburghii	Rroxscaffold_1G00073760
rosa_rugosa	Rorug04G0394700
rosa_samantha	Rh5AG016500 Rh5AG016600 Rh5AG017100 Rh5BG020000 Rh5CG017800 Rh5CG017900 Rh5CG018800 Rh5DG018000
rosa_wichuraiana	Rw2G029690 Rw5G001610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1988
AccB1I	GGYRCC	2 cut(s)	326, 1575
AccB7I	CCANNNNNTGG	1 cut(s)	1652
AccII	CGCG	2 cut(s)	685, 1739
AciI	CCGC	7 cut(s)	209, 611, 1112, 1196, 1293, 1620, 2119
AclWI	GGATC	6 cut(s)	392, 1158, 1797, 1810, 1874, 1887
AcoI	YGGCCR	1 cut(s)	2010
AcsI	RAATTY	2 cut(s)	272, 1581
AcuI	CTGAAG	2 cut(s)	543, 1161
AcvI	CACGTG	1 cut(s)	595
AfaI	GTAC	5 cut(s)	263, 1548, 1657, 1709, 1819
AfiI	CCNNNNNNNGG	6 cut(s)	187, 719, 1034, 1292, 1585, 1652
AflII	CTTAAG	1 cut(s)	832
AgsI	TTSAA	5 cut(s)	55, 146, 1790, 2020, 2079
AjnI	CCWGG	7 cut(s)	186, 910, 1330, 1362, 1502, 1529, 1570
Alw21I	GWGCWC	1 cut(s)	1934
Alw26I	GTCTC	6 cut(s)	107, 228, 499, 934, 2060, 2095
AlwI	GGATC	6 cut(s)	392, 1158, 1797, 1810, 1874, 1887
AoxI	GGCC	4 cut(s)	57, 908, 1885, 2010
ApeKI	GCWGC	8 cut(s)	527, 530, 608, 893, 1427, 1436, 1589, 1691
ApoI	RAATTY	2 cut(s)	272, 1581
Asp700I	GAANNNNTTC	1 cut(s)	1314
AspLEI	GCGC	4 cut(s)	685, 1480, 1610, 1989
AspS9I	GGNCC	8 cut(s)	58, 455, 797, 908, 914, 1025, 1342, 1508
AsuC2I	CCSGG	1 cut(s)	1884
AsuHPI	GGTGA	4 cut(s)	406, 555, 1307, 2117
AvaII	GGWCC	6 cut(s)	455, 797, 914, 1025, 1342, 1508
BaeGI	GKGCMC	1 cut(s)	1578
BamHI	GGATCC	2 cut(s)	1802, 1879
BanI	GGYRCC	2 cut(s)	326, 1575
BanII	GRGCYC	1 cut(s)	1620
BauI	CACGAG	1 cut(s)	1397
BbrPI	CACGTG	1 cut(s)	595
Bbv12I	GWGCWC	1 cut(s)	1934
BbvI	GCAGC	8 cut(s)	514, 517, 595, 905, 1439, 1448, 1601, 1703
BccI	CCATC	8 cut(s)	185, 247, 664, 761, 1085, 1238, 1735, 2013
BceAI	ACGGC	2 cut(s)	621, 1257
BcgI	CGANNNNNNTGC	2 cut(s)	1000, 1034
BciT130I	CCWGG	7 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572
BciVI	GTATCC	1 cut(s)	720
BclI	TGATCA	1 cut(s)	751
BcnI	CCSGG	1 cut(s)	1884
BcoDI	GTCTC	6 cut(s)	107, 228, 499, 934, 2060, 2095
BfaI	CTAG	3 cut(s)	764, 1253, 1889
BfmI	CTRYAG	5 cut(s)	124, 546, 714, 792, 948
BfoI	RGCGCY	2 cut(s)	1481, 1611
BfrI	CTTAAG	1 cut(s)	832
BfuI	GTATCC	1 cut(s)	720
BisI	GCNGC	9 cut(s)	210, 528, 531, 609, 894, 1428, 1437, 1590, 1692
BlsI	GCNGC	9 cut(s)	211, 529, 532, 610, 895, 1429, 1438, 1591, 1693
Bme1390I	CCNGG	8 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572, 1884
Bme18I	GGWCC	6 cut(s)	455, 797, 914, 1025, 1342, 1508
BmgT120I	GGNCC	8 cut(s)	58, 455, 797, 908, 914, 1025, 1342, 1508
BmiI	GGNNCC	8 cut(s)	328, 456, 675, 1344, 1509, 1577, 1804, 1881
BmrFI	CCNGG	8 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572, 1884
BmrI	ACTGGG	2 cut(s)	378, 981
BmsI	GCATC	6 cut(s)	15, 196, 375, 608, 1066, 1182
BmuI	ACTGGG	2 cut(s)	378, 981
BpmI	CTGGAG	4 cut(s)	326, 642, 795, 1513
BpuEI	CTTGAG	1 cut(s)	1659
BpuMI	CCSGG	1 cut(s)	1884
BsaAI	YACGTR	2 cut(s)	595, 840
BsaI	GGTCTC	1 cut(s)	2060
BsaWI	WCCGGW	1 cut(s)	1976
BsaXI	ACNNNNNCTCC	2 cut(s)	615, 645
Bsc4I	CCNNNNNNNGG	6 cut(s)	187, 719, 1034, 1292, 1585, 1652
Bse118I	RCCGGY	1 cut(s)	329
Bse1I	ACTGG	6 cut(s)	121, 309, 384, 471, 629, 987
Bse3DI	GCAATG	3 cut(s)	487, 1302, 1934
BseBI	CCWGG	7 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572
BseGI	GGATG	8 cut(s)	177, 675, 777, 1081, 1214, 1727, 2005, 2063
BseLI	CCNNNNNNNGG	6 cut(s)	187, 719, 1034, 1292, 1585, 1652
BseMI	GCAATG	3 cut(s)	487, 1302, 1934
BseMII	CTCAG	4 cut(s)	183, 525, 1206, 1626
BseNI	ACTGG	6 cut(s)	121, 309, 384, 471, 629, 987
BseRI	GAGGAG	4 cut(s)	255, 327, 1139, 1415
BseSI	GKGCMC	1 cut(s)	1578
BseXI	GCAGC	8 cut(s)	514, 517, 595, 905, 1439, 1448, 1601, 1703
BseYI	CCCAGC	3 cut(s)	466, 515, 872
BsgI	GTGCAG	1 cut(s)	389
Bsh1236I	CGCG	2 cut(s)	685, 1739
BshFI	GGCC	4 cut(s)	59, 910, 1887, 2012
BshNI	GGYRCC	2 cut(s)	326, 1575
BsiHKAI	GWGCWC	1 cut(s)	1934
BsiSI	CCGG	7 cut(s)	330, 1556, 1806, 1884, 1977, 2009, 2098
BslFI	GGGAC	5 cut(s)	1108, 1328, 1485, 1521, 1584
BslI	CCNNNNNNNGG	6 cut(s)	187, 719, 1034, 1292, 1585, 1652
BsmAI	GTCTC	6 cut(s)	107, 228, 499, 934, 2060, 2095
BsmFI	GGGAC	5 cut(s)	1108, 1328, 1485, 1521, 1584
BsnI	GGCC	4 cut(s)	59, 910, 1887, 2012
Bso31I	GGTCTC	1 cut(s)	2060
Bsp1286I	GDGCHC	3 cut(s)	1578, 1620, 1934
Bsp143I	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
Bsp19I	CCATGG	1 cut(s)	1875
BspACI	CCGC	7 cut(s)	209, 611, 1112, 1196, 1293, 1620, 2119
BspANI	GGCC	4 cut(s)	59, 910, 1887, 2012
BspCNI	CTCAG	4 cut(s)	184, 524, 1205, 1625
BspFNI	CGCG	2 cut(s)	685, 1739
BspHI	TCATGA	1 cut(s)	85
BspLI	GGNNCC	8 cut(s)	328, 456, 675, 1344, 1509, 1577, 1804, 1881
BspPI	GGATC	6 cut(s)	392, 1158, 1797, 1810, 1874, 1887
BspQI	GCTCTTC	1 cut(s)	862
BspT107I	GGYRCC	2 cut(s)	326, 1575
BspTI	CTTAAG	1 cut(s)	832
BspTNI	GGTCTC	1 cut(s)	2060
BsrDI	GCAATG	3 cut(s)	487, 1302, 1934
BsrFI	RCCGGY	1 cut(s)	329
BsrI	ACTGG	6 cut(s)	121, 309, 384, 471, 629, 987
BssAI	RCCGGY	1 cut(s)	329
BssMI	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
BssSI	CACGAG	1 cut(s)	1397
BssT1I	CCWWGG	5 cut(s)	277, 289, 941, 1103, 1875
Bst2BI	CACGAG	1 cut(s)	1397
Bst2UI	CCWGG	7 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572
Bst4CI	ACNGT	6 cut(s)	266, 410, 550, 718, 823, 938
Bst6I	CTCTTC	3 cut(s)	862, 1447, 1504
BstAFI	CTTAAG	1 cut(s)	832
BstAPI	GCANNNNNTGC	1 cut(s)	1076
BstBAI	YACGTR	2 cut(s)	595, 840
BstC8I	GCNNGC	7 cut(s)	131, 445, 891, 898, 1291, 1620, 2095
BstDEI	CTNAG	4 cut(s)	192, 511, 1192, 1612
BstDSI	CCRYGG	2 cut(s)	677, 1875
BstF5I	GGATG	8 cut(s)	177, 675, 777, 1081, 1214, 1727, 2005, 2063
BstFNI	CGCG	2 cut(s)	685, 1739
BstH2I	RGCGCY	2 cut(s)	1481, 1611
BstHHI	GCGC	4 cut(s)	685, 1480, 1610, 1989
BstKTI	GATC	8 cut(s)	6, 322, 400, 754, 1153, 1737, 1805, 1882
BstMAI	GTCTC	6 cut(s)	107, 228, 499, 934, 2060, 2095
BstMBI	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
BstNI	CCWGG	7 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572
BstSCI	CCNGG	8 cut(s)	186, 910, 1330, 1362, 1502, 1529, 1570, 1882
BstSFI	CTRYAG	5 cut(s)	124, 546, 714, 792, 948
BstSLI	GKGCMC	1 cut(s)	1578
BstUI	CGCG	2 cut(s)	685, 1739
BstV1I	GCAGC	8 cut(s)	514, 517, 595, 905, 1439, 1448, 1601, 1703
BstX2I	RGATCY	2 cut(s)	1802, 1879
BstYI	RGATCY	2 cut(s)	1802, 1879
BsuI	GTATCC	1 cut(s)	720
BsuRI	GGCC	4 cut(s)	59, 910, 1887, 2012
BtgI	CCRYGG	2 cut(s)	677, 1875
BtsCI	GGATG	8 cut(s)	177, 675, 777, 1081, 1214, 1727, 2005, 2063
BtsI	GCAGTG	3 cut(s)	1086, 1224, 1755
BtsIMutI	CAGTG	8 cut(s)	302, 555, 636, 723, 969, 1086, 1224, 1755
Cac8I	GCNNGC	7 cut(s)	131, 445, 891, 898, 1291, 1620, 2095
CciI	TCATGA	1 cut(s)	85
CfoI	GCGC	4 cut(s)	685, 1480, 1610, 1989
Cfr10I	RCCGGY	1 cut(s)	329
Cfr13I	GGNCC	8 cut(s)	58, 455, 797, 908, 914, 1025, 1342, 1508
CseI	GACGC	2 cut(s)	449, 1728
Csp6I	GTAC	5 cut(s)	262, 1547, 1656, 1708, 1818
CviQI	GTAC	5 cut(s)	262, 1547, 1656, 1708, 1818
DdeI	CTNAG	4 cut(s)	192, 511, 1192, 1612
DpnI	GATC	8 cut(s)	5, 321, 399, 753, 1152, 1736, 1804, 1881
DpnII	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
EaeI	YGGCCR	1 cut(s)	2010
Eam1104I	CTCTTC	3 cut(s)	862, 1447, 1504
EarI	CTCTTC	3 cut(s)	862, 1447, 1504
EciI	GGCGGA	1 cut(s)	1101
Eco130I	CCWWGG	5 cut(s)	277, 289, 941, 1103, 1875
Eco24I	GRGCYC	1 cut(s)	1620
Eco31I	GGTCTC	1 cut(s)	2060
Eco47I	GGWCC	6 cut(s)	455, 797, 914, 1025, 1342, 1508
Eco57I	CTGAAG	2 cut(s)	543, 1161
Eco72I	CACGTG	1 cut(s)	595
EcoO109I	RGGNCCY	2 cut(s)	455, 914
EcoRI	GAATTC	1 cut(s)	272
EcoRII	CCWGG	7 cut(s)	186, 910, 1330, 1362, 1502, 1529, 1570
EcoT14I	CCWWGG	5 cut(s)	277, 289, 941, 1103, 1875
EcoT38I	GRGCYC	1 cut(s)	1620
ErhI	CCWWGG	5 cut(s)	277, 289, 941, 1103, 1875
FalI	AAGNNNNNCTT	2 cut(s)	1444, 1476
FaqI	GGGAC	5 cut(s)	1108, 1328, 1485, 1521, 1584
FauI	CCCGC	3 cut(s)	604, 1189, 1627
FbaI	TGATCA	1 cut(s)	751
Fnu4HI	GCNGC	9 cut(s)	210, 528, 531, 609, 894, 1428, 1437, 1590, 1692
FokI	GGATG	8 cut(s)	164, 682, 764, 1088, 1201, 1714, 1992, 2050
FriOI	GRGCYC	1 cut(s)	1620
Fsp4HI	GCNGC	9 cut(s)	210, 528, 531, 609, 894, 1428, 1437, 1590, 1692
FspBI	CTAG	3 cut(s)	764, 1253, 1889
FspI	TGCGCA	1 cut(s)	1988
GlaI	GCGC	4 cut(s)	684, 1479, 1609, 1988
GluI	GCNGC	9 cut(s)	210, 528, 531, 609, 894, 1428, 1437, 1590, 1692
GsaI	CCCAGC	3 cut(s)	470, 519, 876
GsuI	CTGGAG	4 cut(s)	326, 642, 795, 1513
HaeII	RGCGCY	2 cut(s)	1481, 1611
HaeIII	GGCC	4 cut(s)	59, 910, 1887, 2012
HapII	CCGG	7 cut(s)	330, 1556, 1806, 1884, 1977, 2009, 2098
HgaI	GACGC	2 cut(s)	449, 1728
HhaI	GCGC	4 cut(s)	685, 1480, 1610, 1989
Hin6I	GCGC	4 cut(s)	683, 1478, 1608, 1987
HinP1I	GCGC	4 cut(s)	683, 1478, 1608, 1987
HindIII	AAGCTT	1 cut(s)	131
HpaII	CCGG	7 cut(s)	330, 1556, 1806, 1884, 1977, 2009, 2098
HphI	GGTGA	4 cut(s)	406, 555, 1307, 2117
Hpy166II	GTNNAC	2 cut(s)	775, 1046
Hpy188I	TCNGA	4 cut(s)	439, 1004, 1615, 2068
Hpy188III	TCNNGA	6 cut(s)	86, 269, 764, 812, 1139, 1902
Hpy8I	GTNNAC	2 cut(s)	775, 1046
Hpy99I	CGWCG	1 cut(s)	2072
HpyCH4III	ACNGT	6 cut(s)	266, 410, 550, 718, 823, 938
HpyCH4IV	ACGT	5 cut(s)	260, 594, 839, 1097, 2084
HpyF3I	CTNAG	4 cut(s)	192, 511, 1192, 1612
HpySE526I	ACGT	5 cut(s)	260, 594, 839, 1097, 2084
HspAI	GCGC	4 cut(s)	683, 1478, 1608, 1987
Ksp22I	TGATCA	1 cut(s)	751
Kzo9I	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
LguI	GCTCTTC	1 cut(s)	862
LmnI	GCTCC	5 cut(s)	242, 293, 679, 1847, 2090
Lsp1109I	GCAGC	8 cut(s)	514, 517, 595, 905, 1439, 1448, 1601, 1703
LweI	GCATC	6 cut(s)	15, 196, 375, 608, 1066, 1182
MaeI	CTAG	3 cut(s)	764, 1253, 1889
MaeII	ACGT	5 cut(s)	260, 594, 839, 1097, 2084
MaeIII	GTNAC	4 cut(s)	590, 644, 823, 2123
MalI	GATC	8 cut(s)	5, 321, 399, 753, 1152, 1736, 1804, 1881
MboI	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
MboII	GAAGA	7 cut(s)	499, 827, 879, 989, 1464, 1491, 1922
MfeI	CAATTG	1 cut(s)	747
MflI	RGATCY	2 cut(s)	1802, 1879
MhlI	GDGCHC	3 cut(s)	1578, 1620, 1934
MlyI	GAGTC	3 cut(s)	1102, 1528, 1606
MmeI	TCCRAC	2 cut(s)	1041, 2091
MroXI	GAANNNNTTC	1 cut(s)	1314
MseI	TTAA	6 cut(s)	8, 540, 728, 833, 1352, 1625
MspA1I	CMGCKG	2 cut(s)	515, 1196
MspCI	CTTAAG	1 cut(s)	832
MspI	CCGG	7 cut(s)	330, 1556, 1806, 1884, 1977, 2009, 2098
MspR9I	CCNGG	8 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572, 1884
MunI	CAATTG	1 cut(s)	747
MvaI	CCWGG	7 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572
MvnI	CGCG	2 cut(s)	685, 1739
NciI	CCSGG	1 cut(s)	1884
NcoI	CCATGG	1 cut(s)	1875
NdeII	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
NlaIV	GGNNCC	8 cut(s)	328, 456, 675, 1344, 1509, 1577, 1804, 1881
NmeAIII	GCCGAG	1 cut(s)	476
NmuCI	GTSAC	2 cut(s)	590, 2123
NsbI	TGCGCA	1 cut(s)	1988
PagI	TCATGA	1 cut(s)	85
PasI	CCCWGGG	1 cut(s)	1571
PciSI	GCTCTTC	1 cut(s)	862
PdmI	GAANNNNTTC	1 cut(s)	1314
PfeI	GAWTC	7 cut(s)	80, 359, 462, 1200, 1494, 1723, 1854
PflMI	CCANNNNNTGG	1 cut(s)	1652
PfoI	TCCNGGA	1 cut(s)	1529
PkrI	GCNGC	9 cut(s)	211, 529, 532, 610, 895, 1429, 1438, 1591, 1693
PleI	GAGTC	3 cut(s)	1102, 1528, 1605
PmaCI	CACGTG	1 cut(s)	595
PmlI	CACGTG	1 cut(s)	595
PpsI	GAGTC	3 cut(s)	1102, 1528, 1605
Ppu21I	YACGTR	2 cut(s)	595, 840
PpuMI	RGGWCCY	2 cut(s)	455, 914
Psp5II	RGGWCCY	2 cut(s)	455, 914
Psp6I	CCWGG	7 cut(s)	186, 910, 1330, 1362, 1502, 1529, 1570
PspCI	CACGTG	1 cut(s)	595
PspFI	CCCAGC	3 cut(s)	466, 515, 872
PspGI	CCWGG	7 cut(s)	186, 910, 1330, 1362, 1502, 1529, 1570
PspN4I	GGNNCC	8 cut(s)	328, 456, 675, 1344, 1509, 1577, 1804, 1881
PspPI	GGNCC	8 cut(s)	58, 455, 797, 908, 914, 1025, 1342, 1508
PspPPI	RGGWCCY	2 cut(s)	455, 914
PsuI	RGATCY	2 cut(s)	1802, 1879
PvuII	CAGCTG	1 cut(s)	515
RsaI	GTAC	5 cut(s)	263, 1548, 1657, 1709, 1819
RsaNI	GTAC	5 cut(s)	262, 1547, 1656, 1708, 1818
SapI	GCTCTTC	1 cut(s)	862
SaqAI	TTAA	6 cut(s)	8, 540, 728, 833, 1352, 1625
SatI	GCNGC	9 cut(s)	210, 528, 531, 609, 894, 1428, 1437, 1590, 1692
Sau3AI	GATC	8 cut(s)	3, 319, 397, 751, 1150, 1734, 1802, 1879
Sau96I	GGNCC	8 cut(s)	58, 455, 797, 908, 914, 1025, 1342, 1508
SchI	GAGTC	3 cut(s)	1102, 1528, 1606
ScrFI	CCNGG	8 cut(s)	188, 912, 1332, 1364, 1504, 1531, 1572, 1884
SduI	GDGCHC	3 cut(s)	1578, 1620, 1934
SfaNI	GCATC	6 cut(s)	15, 196, 375, 608, 1066, 1182
SfcI	CTRYAG	5 cut(s)	124, 546, 714, 792, 948
SgrAI	CRCCGGYG	1 cut(s)	329
SinI	GGWCC	6 cut(s)	455, 797, 914, 1025, 1342, 1508
SmlI	CTYRAG	2 cut(s)	832, 1674
SmoI	CTYRAG	2 cut(s)	832, 1674
SsiI	CCGC	7 cut(s)	209, 611, 1112, 1196, 1293, 1620, 2119
SspI	AATATT	1 cut(s)	1379
SspMI	CTAG	3 cut(s)	764, 1253, 1889
StyD4I	CCNGG	8 cut(s)	186, 910, 1330, 1362, 1502, 1529, 1570, 1882
StyI	CCWWGG	5 cut(s)	277, 289, 941, 1103, 1875
TaaI	ACNGT	6 cut(s)	266, 410, 550, 718, 823, 938
TaiI	ACGT	5 cut(s)	263, 597, 842, 1100, 2087
TaqI	TCGA	8 cut(s)	460, 865, 920, 1020, 1122, 1420, 1870, 2030
TaqII	GACCGA	1 cut(s)	1042
TatI	WGTACW	3 cut(s)	1546, 1707, 1817
TauI	GCSGC	1 cut(s)	212
TfiI	GAWTC	7 cut(s)	80, 359, 462, 1200, 1494, 1723, 1854
Tru1I	TTAA	6 cut(s)	8, 540, 728, 833, 1352, 1625
Tru9I	TTAA	6 cut(s)	8, 540, 728, 833, 1352, 1625
TscAI	CASTG	8 cut(s)	309, 555, 636, 723, 976, 1086, 1231, 1762
TseFI	GTSAC	2 cut(s)	590, 2123
TseI	GCWGC	8 cut(s)	527, 530, 608, 893, 1427, 1436, 1589, 1691
Tsp45I	GTSAC	2 cut(s)	590, 2123
TspDTI	ATGAA	8 cut(s)	465, 819, 951, 1291, 1398, 1456, 1707, 1923
TspGWI	ACGGA	4 cut(s)	1224, 1794, 1815, 2085
TspRI	CASTG	8 cut(s)	309, 555, 636, 723, 976, 1086, 1231, 1762
Van91I	CCANNNNNTGG	1 cut(s)	1652
Vha464I	CTTAAG	1 cut(s)	832
VpaK11BI	GGWCC	6 cut(s)	455, 797, 914, 1025, 1342, 1508
XapI	RAATTY	2 cut(s)	272, 1581
XbaI	TCTAGA	1 cut(s)	763
XmnI	GAANNNNTTC	1 cut(s)	1314
XspI	CTAG	3 cut(s)	764, 1253, 1889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.