Rh5CG018800

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0006269
Physical Location & Seq
Forward (+)
1226133 .. 1229825
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG018800.1

Sequence Viewer

Length: 1611 bp
ATGGAAGAAGACTCGGTTGCAATCAATGGAGGCTCTCCATCTTCACTCCTCAACCTAAATAGTGAAGTTTCGAAGGCTCATGTAAAGCAATTGGAGGATTCTCATGATCAACTTCTACAGAGCAATCTTGAGACCGGAAGCTACAGAAATCTTTACAGCTTTAAACACGTTGCAAACGGGTTTGCTGTCCATACAACCCCTTCTCAGAGGAGCAAAGTTGATGACAAATTGACAACGTACACTCCTCGCTTCCTCGAGTTACCTGAAGGAGTATGGACACAAGAAGGAGGCCAACGTAATGCTGGTGAAGGAATTGTGATTGGTTTTGTGGATAGTGGCATCAATCCTGCACATCTAAGTTTTGCTTATAATCCTTTGAGCCCTTTCACCTCCAATATTTCTCATTTTTCGGGCGCTTGTGAGACTGTTCCTCTATTCCCAGCAACTTCATGCAATGGTAAAATTGTTTCAGCTAAGTTTTTCTCAGCTGGGGCTCGAGCCATTGCTACTCTCAACGCTTCGGTGGATTTTCTTTCACCTTCGACGCAGATGGACATGGCAGTTGCTGCTGGAAATGTTGGAGTTCCTGTGGTTGTGAATGGCTTCAGTTATGGACGAGCTAGTAGAATAGCACCACGTGCGCGGATTGCTGTATATAAAGCTGTGTATCCTACAGTAGGAACTTTGGCAGATGTAGTTTCTGCAATTGATGAAGCAATTCTAGATGGAGTGGATGTCCTAGCACTCTCTGTCGGACCAGATGAACCGCCATTGGGTAAAGTGACCTTCTTAAGCATGTTTGATGTGTTCATGCTGTATGCTCGACGAGCTGGGGTCTTCGTGGTGCAAGCAGCAGGTAATCGAGGGCCAGACCCTTCAAGTGTAGTGTCTTACAGCCCTTGGGCAGTAGGTGTAGCTTCTTCCGGCACAGATAGAATTTATCCTTCTTCTATTGTTCTTGGAAATGGAGCAAGAATTGAAGGTGTGGGATTATCAGGACCAACTTTTGGATATAGGTTACTTCAACATAGGTTGGTTTTGGCCAAGGATGCAATTAATCCAATTGGGTCATTCCCAAGAACCCCACCCTATGTTGAAGAGTGCCAATTTCCACAAGCATTGGATCCTAGGTTAGTTATAGGTAGTATTGTCATCTGCACATTCTCGGAGGGATTCTATAACAGGACATCCACCCTTTTTGCCATCATCAACACGGCAAGAGCTCTCGGATTTATGGGTTTTGTTCTTGTTGCAAATCCAGCCTATGGTGACTTCATTGCAGAGCCTGTTCCTTTTGCTGTTTCTGGCATTTTGGTCCCAAATGTAACCAATTCCCAGGTTATATTACAATACTATGAACAACAAACATTCAAGGATGAGAGAGGATTTCCTACAAGACTTGCAGCGAGAGCATCAATCGGAGAAGGAAGAGCTGCTTCTTTTACGGGCCAAGCACCTATTGTTAGCAGATTCTCTTCGAGAGGACCAGATTTCATTGACAGAGACAGGAAGCCTGCTGATGTACTAAAGCCTGATATTCTTGCCCCGGGGCACCAAATCTGGGGAGCTTGGAGCTCTATTAGTGCCTTTAGAACCCTCATTAGTGGGTGA

Protein Analysis

536

Amino Acids

57.23

Weight (kDa)

6.27

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 102 - 519 4.3e-12 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 1551
AccB7I CCANNNNNTGG 2 cut(s) 1007, 1265
AccII CGCG 1 cut(s) 643
AciI CCGC 2 cut(s) 643, 767
AclWI GGATC 2 cut(s) 1118, 1131
AcoI YGGCCR 1 cut(s) 1041
AcsI RAATTY 1 cut(s) 936
AcuI CTGAAG 2 cut(s) 285, 589
AcvI CACGTG 1 cut(s) 638
AdeI CACNNNGTG 1 cut(s) 638
AfaI GTAC 2 cut(s) 239, 1524
AfiI CCNNNNNNNGG 5 cut(s) 677, 773, 1007, 1265, 1561
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 5 cut(s) 879, 980, 1025, 1097, 1372
AjnI CCWGG 1 cut(s) 1335
Alw21I GWGCWC 2 cut(s) 1225, 1577
Alw26I GTCTC 3 cut(s) 125, 416, 1497
AlwI GGATC 2 cut(s) 1118, 1131
AlwNI CAGNNNCTG 2 cut(s) 566, 1286
Ama87I CYCGRG 3 cut(s) 254, 495, 1546
AoxI GGCC 4 cut(s) 289, 866, 1041, 1447
ApeKI GCWGC 4 cut(s) 566, 851, 1403, 1433
ApoI RAATTY 1 cut(s) 936
AseI ATTAAT 1 cut(s) 1056
Asp700I GAANNNNTTC 2 cut(s) 602, 717
AspA2I CCTAGG 1 cut(s) 1127
AspLEI GCGC 2 cut(s) 416, 643
AspS9I GGNCC 6 cut(s) 755, 866, 998, 1315, 1447, 1484
AsuC2I CCSGG 2 cut(s) 1547, 1548
AsuHPI GGTGA 4 cut(s) 317, 379, 528, 1280
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 3 cut(s) 254, 495, 1546
AvaII GGWCC 4 cut(s) 755, 998, 1315, 1484
AvrII CCTAGG 1 cut(s) 1127
BaeGI GKGCMC 1 cut(s) 1554
BalI TGGCCA 1 cut(s) 1043
BamHI GGATCC 1 cut(s) 1123
BanI GGYRCC 1 cut(s) 1551
BanII GRGCYC 4 cut(s) 383, 496, 1225, 1577
BbrPI CACGTG 1 cut(s) 638
BbsI GAAGAC 2 cut(s) 15, 829
Bbv12I GWGCWC 2 cut(s) 1225, 1577
BbvI GCAGC 4 cut(s) 553, 863, 1415, 1420
BccI CCATC 4 cut(s) 46, 544, 719, 1211
BceAI ACGGC 1 cut(s) 1230
BciT130I CCWGG 1 cut(s) 1337
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 2 cut(s) 1547, 1548
BcoDI GTCTC 3 cut(s) 125, 416, 1497
BfaI CTAG 4 cut(s) 621, 722, 740, 1128
BfmI CTRYAG 3 cut(s) 116, 142, 672
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 790
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 678
BisI GCNGC 4 cut(s) 567, 852, 1404, 1434
BlnI CCTAGG 1 cut(s) 1127
BlsI GCNGC 4 cut(s) 568, 853, 1405, 1435
Bme1390I CCNGG 3 cut(s) 1337, 1547, 1548
Bme18I GGWCC 4 cut(s) 755, 998, 1315, 1484
BmeT110I CYCGRG 3 cut(s) 254, 495, 1546
BmgT120I GGNCC 6 cut(s) 755, 866, 998, 1315, 1447, 1484
BmiI GGNNCC 3 cut(s) 1125, 1317, 1553
BmrFI CCNGG 3 cut(s) 1337, 1547, 1548
BmsI GCATC 3 cut(s) 348, 1039, 1421
BpiI GAAGAC 2 cut(s) 15, 829
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 2 cut(s) 1547, 1548
BsaAI YACGTR 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 7 cut(s) 899, 1044, 1127, 1335, 1545, 1546, 1547
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 6 cut(s) 226, 256, 573, 603, 720, 750
Bsc4I CCNNNNNNNGG 5 cut(s) 677, 773, 1007, 1265, 1561
Bse3DI GCAATG 3 cut(s) 460, 501, 1275
BseBI CCWGG 1 cut(s) 1337
BseDI CCNNGG 7 cut(s) 899, 1044, 1127, 1335, 1545, 1546, 1547
BseGI GGATG 4 cut(s) 739, 1054, 1187, 1381
BseLI CCNNNNNNNGG 5 cut(s) 677, 773, 1007, 1265, 1561
BseMI GCAATG 3 cut(s) 460, 501, 1275
BseMII CTCAG 2 cut(s) 218, 498
BseRI GAGGAG 3 cut(s) 38, 223, 234
BseSI GKGCMC 1 cut(s) 1554
BseXI GCAGC 4 cut(s) 553, 863, 1415, 1420
BseYI CCCAGC 3 cut(s) 439, 488, 830
BsgI GTGCAG 2 cut(s) 333, 1141
Bsh1236I CGCG 1 cut(s) 643
BshFI GGCC 4 cut(s) 291, 868, 1043, 1449
BshNI GGYRCC 1 cut(s) 1551
BsiHKAI GWGCWC 2 cut(s) 1225, 1577
BsiHKCI CYCGRG 3 cut(s) 254, 495, 1546
BsiSI CCGG 3 cut(s) 135, 924, 1547
BslFI GGGAC 1 cut(s) 1301
BslI CCNNNNNNNGG 5 cut(s) 677, 773, 1007, 1265, 1561
BsmAI GTCTC 3 cut(s) 125, 416, 1497
BsmFI GGGAC 1 cut(s) 1301
BsnI GGCC 4 cut(s) 291, 868, 1043, 1449
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 3 cut(s) 254, 495, 1546
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 5 cut(s) 383, 496, 1225, 1554, 1577
Bsp143I GATC 2 cut(s) 106, 1123
BspACI CCGC 2 cut(s) 643, 767
BspANI GGCC 4 cut(s) 291, 868, 1043, 1449
BspCNI CTCAG 2 cut(s) 217, 497
BspFNI CGCG 1 cut(s) 643
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 1125, 1317, 1553
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 2 cut(s) 1118, 1131
BspQI GCTCTTC 1 cut(s) 1423
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 1551
BspTI CTTAAG 1 cut(s) 790
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 3 cut(s) 460, 501, 1275
BssECI CCNNGG 7 cut(s) 899, 1044, 1127, 1335, 1545, 1546, 1547
BssMI GATC 2 cut(s) 106, 1123
BssT1I CCWWGG 3 cut(s) 899, 1044, 1127
Bst2UI CCWGG 1 cut(s) 1337
Bst4CI ACNGT 2 cut(s) 427, 676
Bst6I CTCTTC 3 cut(s) 1092, 1423, 1480
BstAFI CTTAAG 1 cut(s) 790
BstAPI GCANNNNNTGC 2 cut(s) 566, 638
BstBAI YACGTR 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 849, 1515
BstDEI CTNAG 4 cut(s) 204, 356, 474, 484
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 4 cut(s) 739, 1054, 1187, 1381
BstFNI CGCG 1 cut(s) 643
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 2 cut(s) 416, 643
BstKTI GATC 2 cut(s) 109, 1126
BstMAI GTCTC 3 cut(s) 125, 416, 1497
BstMBI GATC 2 cut(s) 106, 1123
BstMWI GCNNNNNNNGC 7 cut(s) 566, 638, 647, 827, 1049, 1259, 1409
BstNI CCWGG 1 cut(s) 1337
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 3 cut(s) 1335, 1545, 1546
BstSFI CTRYAG 3 cut(s) 116, 142, 672
BstSLI GKGCMC 1 cut(s) 1554
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 4 cut(s) 553, 863, 1415, 1420
BstV2I GAAGAC 2 cut(s) 15, 829
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 4 cut(s) 291, 868, 1043, 1449
BtsCI GGATG 4 cut(s) 739, 1054, 1187, 1381
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 849, 1515
CaiI CAGNNNCTG 2 cut(s) 566, 1286
CciI TCATGA 1 cut(s) 103
CfoI GCGC 2 cut(s) 416, 643
Cfr13I GGNCC 6 cut(s) 755, 866, 998, 1315, 1447, 1484
Cfr9I CCCGGG 1 cut(s) 1546
CseI GACGC 1 cut(s) 553
Csp6I GTAC 2 cut(s) 238, 1523
CspCI CAANNNNNGTGG 4 cut(s) 1101, 1136, 1180, 1215
CviAII CATG 6 cut(s) 80, 104, 450, 556, 796, 811
CviQI GTAC 2 cut(s) 238, 1523
DdeI CTNAG 4 cut(s) 204, 356, 474, 484
DpnI GATC 2 cut(s) 108, 1125
DpnII GATC 2 cut(s) 106, 1123
DraI TTTAAA 1 cut(s) 163
DraIII CACNNNGTG 1 cut(s) 638
EaeI YGGCCR 1 cut(s) 1041
Eam1104I CTCTTC 3 cut(s) 1092, 1423, 1480
EarI CTCTTC 3 cut(s) 1092, 1423, 1480
Ecl136II GAGCTC 2 cut(s) 1223, 1575
Eco130I CCWWGG 3 cut(s) 899, 1044, 1127
Eco24I GRGCYC 4 cut(s) 383, 496, 1225, 1577
Eco31I GGTCTC 1 cut(s) 125
Eco47I GGWCC 4 cut(s) 755, 998, 1315, 1484
Eco53kI GAGCTC 2 cut(s) 1223, 1575
Eco57I CTGAAG 2 cut(s) 285, 589
Eco72I CACGTG 1 cut(s) 638
Eco88I CYCGRG 3 cut(s) 254, 495, 1546
EcoICRI GAGCTC 2 cut(s) 1223, 1575
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 1335
EcoT14I CCWWGG 3 cut(s) 899, 1044, 1127
EcoT38I GRGCYC 4 cut(s) 383, 496, 1225, 1577
ErhI CCWWGG 3 cut(s) 899, 1044, 1127
FaeI CATG 6 cut(s) 83, 107, 453, 559, 799, 814
FalI AAGNNNNNCTT 4 cut(s) 349, 381, 1420, 1452
FaqI GGGAC 1 cut(s) 1301
FatI CATG 6 cut(s) 79, 103, 449, 555, 795, 810
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 4 cut(s) 567, 852, 1404, 1434
FokI GGATG 4 cut(s) 746, 1061, 1174, 1388
FriOI GRGCYC 4 cut(s) 383, 496, 1225, 1577
Fsp4HI GCNGC 4 cut(s) 567, 852, 1404, 1434
FspBI CTAG 4 cut(s) 621, 722, 740, 1128
GlaI GCGC 2 cut(s) 415, 642
GluI GCNGC 4 cut(s) 567, 852, 1404, 1434
GsaI CCCAGC 3 cut(s) 443, 492, 834
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 4 cut(s) 291, 868, 1043, 1449
HapII CCGG 3 cut(s) 135, 924, 1547
HgaI GACGC 1 cut(s) 553
HhaI GCGC 2 cut(s) 416, 643
Hin1II CATG 6 cut(s) 83, 107, 453, 559, 799, 814
Hin6I GCGC 2 cut(s) 414, 641
HinP1I GCGC 2 cut(s) 414, 641
HinfI GANTC 4 cut(s) 11, 98, 1173, 1470
HpaII CCGG 3 cut(s) 135, 924, 1547
HphI GGTGA 4 cut(s) 317, 379, 528, 1280
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 5 cut(s) 207, 755, 1168, 1229, 1421
Hpy188III TCNNGA 5 cut(s) 104, 128, 722, 996, 1479
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 2 cut(s) 547, 828
HpyCH4III ACNGT 2 cut(s) 427, 676
HpyCH4IV ACGT 4 cut(s) 168, 236, 295, 637
HpyF10VI GCNNNNNNNGC 7 cut(s) 566, 638, 647, 827, 1049, 1259, 1409
HpyF3I CTNAG 4 cut(s) 204, 356, 474, 484
HpySE526I ACGT 4 cut(s) 168, 236, 295, 637
Hsp92II CATG 6 cut(s) 83, 107, 453, 559, 799, 814
HspAI GCGC 2 cut(s) 414, 641
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 2 cut(s) 106, 1123
LguI GCTCTTC 1 cut(s) 1423
LmnI GCTCC 4 cut(s) 210, 968, 1565, 1572
Lsp1109I GCAGC 4 cut(s) 553, 863, 1415, 1420
LweI GCATC 3 cut(s) 348, 1039, 1421
MaeI CTAG 4 cut(s) 621, 722, 740, 1128
MaeII ACGT 4 cut(s) 168, 236, 295, 637
MaeIII GTNAC 5 cut(s) 258, 781, 1017, 1268, 1324
MalI GATC 2 cut(s) 108, 1125
MboI GATC 2 cut(s) 106, 1123
MboII GAAGA 9 cut(s) 17, 20, 33, 829, 912, 939, 1109, 1440, 1467
MfeI CAATTG 3 cut(s) 89, 705, 1062
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 5 cut(s) 383, 496, 1225, 1554, 1577
MlsI TGGCCA 1 cut(s) 1043
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 559, 733
Mox20I TGGCCA 1 cut(s) 1043
MroXI GAANNNNTTC 2 cut(s) 602, 717
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 3 cut(s) 162, 791, 1056
Msp20I TGGCCA 1 cut(s) 1043
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 3 cut(s) 135, 924, 1547
MspR9I CCNGG 3 cut(s) 1337, 1547, 1548
MunI CAATTG 3 cut(s) 89, 705, 1062
MvaI CCWGG 1 cut(s) 1337
MvnI CGCG 1 cut(s) 643
MwoI GCNNNNNNNGC 7 cut(s) 566, 638, 647, 827, 1049, 1259, 1409
NciI CCSGG 2 cut(s) 1547, 1548
NdeII GATC 2 cut(s) 106, 1123
NlaIII CATG 6 cut(s) 83, 107, 453, 559, 799, 814
NlaIV GGNNCC 3 cut(s) 1125, 1317, 1553
NmuCI GTSAC 2 cut(s) 781, 1268
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 71
PaeR7I CTCGAG 2 cut(s) 254, 495
PagI TCATGA 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 1423
PdmI GAANNNNTTC 2 cut(s) 602, 717
PfeI GAWTC 3 cut(s) 98, 1173, 1470
PflMI CCANNNNNTGG 2 cut(s) 1007, 1265
PkrI GCNGC 4 cut(s) 568, 853, 1405, 1435
PleI GAGTC 1 cut(s) 5
PmaCI CACGTG 1 cut(s) 638
PmlI CACGTG 1 cut(s) 638
PpsI GAGTC 1 cut(s) 5
Ppu21I YACGTR 1 cut(s) 638
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 369
Psp124BI GAGCTC 2 cut(s) 1225, 1577
Psp6I CCWGG 1 cut(s) 1335
PspCI CACGTG 1 cut(s) 638
PspFI CCCAGC 3 cut(s) 439, 488, 830
PspGI CCWGG 1 cut(s) 1335
PspN4I GGNNCC 3 cut(s) 1125, 1317, 1553
PspPI GGNCC 6 cut(s) 755, 866, 998, 1315, 1447, 1484
PspXI VCTCGAGB 2 cut(s) 254, 495
PstNI CAGNNNCTG 2 cut(s) 566, 1286
PsuI RGATCY 1 cut(s) 1123
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 2 cut(s) 239, 1524
RsaNI GTAC 2 cut(s) 238, 1523
SacI GAGCTC 2 cut(s) 1225, 1577
SapI GCTCTTC 1 cut(s) 1423
SaqAI TTAA 3 cut(s) 162, 791, 1056
SatI GCNGC 4 cut(s) 567, 852, 1404, 1434
Sau3AI GATC 2 cut(s) 106, 1123
Sau96I GGNCC 6 cut(s) 755, 866, 998, 1315, 1447, 1484
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 3 cut(s) 1337, 1547, 1548
SduI GDGCHC 5 cut(s) 383, 496, 1225, 1554, 1577
SfaNI GCATC 3 cut(s) 348, 1039, 1421
SfcI CTRYAG 3 cut(s) 116, 142, 672
Sfr274I CTCGAG 2 cut(s) 254, 495
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 4 cut(s) 755, 998, 1315, 1484
SlaI CTCGAG 2 cut(s) 254, 495
SmaI CCCGGG 1 cut(s) 1548
SmlI CTYRAG 4 cut(s) 128, 254, 495, 790
SmoI CTYRAG 4 cut(s) 128, 254, 495, 790
SsiI CCGC 2 cut(s) 643, 767
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 621, 722, 740, 1128
SstI GAGCTC 2 cut(s) 1225, 1577
StyD4I CCNGG 3 cut(s) 1335, 1545, 1546
StyI CCWWGG 3 cut(s) 899, 1044, 1127
TaaI ACNGT 2 cut(s) 427, 676
TaiI ACGT 4 cut(s) 171, 239, 298, 640
TaqI TCGA 7 cut(s) 71, 255, 496, 542, 823, 862, 1478
TatI WGTACW 1 cut(s) 1522
TfiI GAWTC 3 cut(s) 98, 1173, 1470
Tru1I TTAA 3 cut(s) 162, 791, 1056
Tru9I TTAA 3 cut(s) 162, 791, 1056
TseFI GTSAC 2 cut(s) 781, 1268
TseI GCWGC 4 cut(s) 566, 851, 1403, 1433
Tsp45I GTSAC 2 cut(s) 781, 1268
TspDTI ATGAA 7 cut(s) 438, 726, 777, 799, 1264, 1371, 1483
TspMI CCCGGG 1 cut(s) 1546
Van91I CCANNNNNTGG 2 cut(s) 1007, 1265
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 4 cut(s) 755, 998, 1315, 1484
VspI ATTAAT 1 cut(s) 1056
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 1 cut(s) 936
XbaI TCTAGA 1 cut(s) 721
XceI RCATGY 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XhoI CTCGAG 2 cut(s) 254, 495
XmaI CCCGGG 1 cut(s) 1546
XmaJI CCTAGG 1 cut(s) 1127
XmnI GAANNNNTTC 2 cut(s) 602, 717
XspI CTAG 4 cut(s) 621, 722, 740, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.