Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0006269

Physical Location & Seq

Genomic Coordinates Forward (+)

1188189 .. 1191533

Gene Structure 3345 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG016500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2346 bp

ATGGAAGGAGACTCACTTGCATTCAATGGAGGCTCTCCATCTTCACGCCTCAACCTAAACAGTGAAATTTCGAAGGCTCATGCAAAGCAATTGGAGGATTCTCATGATCAACTTCTACAGAGCAATCTTGAGACTGGAAGCTACAGCAAGCTTTACAGCTTTAAACACGTTGCAAACGGCTTTGCTGTCCATACAACCCCTTCACAGGCTGAAAAGCTGAAATATGCTCCGAGTGTAAAGCTGGTTGAGAGAGATAGAGGGGCGAAGTTGATGACAACGTACACTCCTCGCTTCCTTGAGTTACCTGAAGGAGTATGGACACAAGAAGGAGGCCAACGTAACGCTGGTGAAGGAATTGTGATTGGTTTTGTGGATTCTGGCATCAACCCTGTACATCTAAGTTTTGCTTATAATCCTCTGAGCCCTTTCACCTCTAATATTTCTCATTTTTCGGGTGCTTGTGAGACTGGTCCTCTATTCCCAGCAACTTCATGCAATGGTAAAATAGTTTCAGCCAAGTTTTTCTCAGCCGGGGCTCTAGCCATTGCTACTCTCAACAGTTCAGTGGATTTTCTTTCACCTTTTGACTCAGATGGACATGGCAGTCATGTGGCTTCGGTTGCTGCTGGAAATGCTGGAGTTCCTGTGGTTGTGAATGGCTTCTATTATGGACAAGCTAGTGGAATGGCACCACGTGCGCGGATTGCTGTATATAAAGCTGTGTATCCTACAGTAGGAACTTTGGCAGATGTAGTTTCTGCAATTGATGAAGCAATTCGAGATGGAGTGGACGTCCTAGCACTCTCTGTCGGACCAGACGAACCGCCATTGGGTAGAGTGACCTTCTTAAGCATGTTTGATGTGTTCATGTTATATGCTCGACGAGCTGGGGTTTTTGTGGTGCAAGCAGCAGGTAATCGAGGGCCAGACCCTTCAAGTGTAGTGTCTTACAGCCCTTGGGCAGTAGGTGTAGCTTCTTCCGGCACAGATAGAATTTATCCTTGTTCTATTGTTCTTGGAAATGGAGCAAGAATTGAAGGTGTGGGATTATCAGGACCAACTTTTGGATATAGGTTACTTCAACATAGGTTGGTTTTGGCCAAGGATGCAATTAATCCAATTGGGTCATTCCCAAGAACCCCACCCTATGTTGAAGAGTGCCAATTTCCACAAGCATTGGATCCTAGGTTAGTTATAGGTAGTATTGTCATCTGCACATTCTCGGAGGGATTCTATAACAGGACATCCACCCTTACTGCCATCATCAACACGGCAAGAGCTCTCGGATTTATGGGTTTTGTTCTTGTTGCAAATCCAGCCTATGGTGACTTCATTGCAGAGCCTGTTCCTTTTGCTGTTTCTGGCATTTTGGTCCCAAATGTAACCAATTCCCAGGTTATATTACAATACTATGAACAACAAACATTCAAGGATGAGAGAGGATTTCCTACAAGACTTGCAGCGAGAGCATCAATCGGAGAAGGAAGAGCTGCTTCTTTTACGGGCCAAGCACCTATTGTTAGCAGATTCTCTTCGAGAGGACCAGATTTCATTGACAGAGACAGGAAGCCTGCTGATGTACTAAAGCCTGATATTCTTGCCCCGGGGCACCAAATCTGGGGAGCTTGGAGCCCCATTAGTGCCTTAGAACCGTCATTAGTGGGACAAAGTTTTGCACTACTTTCTGGTACAAGCATGGCAACACCTCATATCGCTGGAATAGCAGCACTTATTAAGCAGTACAATCCTTCATGGACTCCTTCCATGATTGCATCTGCGATTTCCACCACGGCAACCAAATATGATAAAAATGGAGAACTGATAATGGCTGAAGGATCTGATATTGGCAGTAAATATCCCTCCACTCCTTTTGATTTTGGAGCTGGCTTTGTCAGTCCAAGTCATGCCATGGATCCGGGTCTAGTCTTATCTTCAGGATATGAAGACTACATCAGCTTCTTGTGCTCATTGCATGATACCAATCCAAGTACAATAAGAACTGTCACTGGAGAGTTGTGCAACAAGCCACTTGACCATCCAGCCAATCTGAACCTTCCTTCAGTAACAATATCAGCATTGAATGGGTCTCAAATAGTACGAAGGGTTTTCATGAACGTAGAGAGCAAGCCAGAGACTTACTCTAGCTCGGTGCTGCCACCAAATGGGACAACAGTAGACATATCTCCTCCTTCGTTTACCATAGCTCCACAAGGTACTCAAGAGCTAGGTATACAGTTCAATGTGACCCAAGCAATGGATCAATTCACCTATGGTGAAATCGTTCTTACAGGAAGTTTGAATCATATTGTCAGGATACCTTTATCAGTTTTTCCAGTTTCAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0032501 GO:0032502 GO:0042335 GO:0043170 GO:0044238 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

781

Amino Acids

83.08

Weight (kDa)

5.79

Isoelectric Point (pI)

32.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	27 - 90	5.7e-12	Peptidase inhibitor I9
Peptidase_S8	PF00082	116 - 605	6.8e-41	Subtilase family
fn3_6	PF17766	682 - 776	2.3e-16	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006269)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62340
fragaria_vesca	FvH4_3g01420
malus_domestica	MD05G1352200.v1.1 MD10G1326200.v1.1
prunus_persica	Prupe.4G015500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002151 RchiOBHm_Chr5g0002191
rosa_laevigata	RLG00000031001 RLG00000031002 RLG00000031007
rosa_multiflora	Rmu_co8154878.1_g000001 Rmu_sc0001492.1_g000001 Rmu_sc0007900.1_g000001 Rmu_sc0013155.1_g000003 Rmu_ssc0000001.1_g000005
rosa_roxburghii	Rroxscaffold_1G00073760
rosa_rugosa	Rorug04G0394700
rosa_samantha	Rh5AG016500 Rh5AG016600 Rh5AG017100 Rh5BG020000 Rh5CG017800 Rh5CG017900 Rh5CG018800 Rh5DG018000
rosa_wichuraiana	Rw2G029690 Rw5G001610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	411
AasI	GACNNNNNNGTC	1 cut(s)	603
AatII	GACGTC	1 cut(s)	795
Acc36I	ACCTGC	1 cut(s)	902
AccB1I	GGYRCC	2 cut(s)	688, 1608
AccB7I	CCANNNNNTGG	4 cut(s)	1064, 1322, 2162, 2254
AccI	GTMKAC	2 cut(s)	2175, 2230
AccII	CGCG	1 cut(s)	700
AciI	CCGC	2 cut(s)	700, 824
AclWI	GGATC	6 cut(s)	1175, 1188, 1843, 1907, 1920, 2265
AcoI	YGGCCR	1 cut(s)	1098
AcsI	RAATTY	2 cut(s)	66, 993
AcuI	CTGAAG	4 cut(s)	327, 1851, 1917, 2043
AcvI	CACGTG	1 cut(s)	695
AcyI	GRCGYC	1 cut(s)	792
AdeI	CACNNNGTG	1 cut(s)	695
AfaI	GTAC	8 cut(s)	281, 393, 1581, 1690, 1742, 1990, 2097, 2215
AfiI	CCNNNNNNNGG	8 cut(s)	205, 734, 830, 1064, 1322, 1618, 2162, 2254
AflII	CTTAAG	1 cut(s)	847
AflIII	ACRYGT	1 cut(s)	166
AjnI	CCWGG	1 cut(s)	1392
Alw21I	GWGCWC	2 cut(s)	1282, 1967
Alw26I	GTCTC	6 cut(s)	3, 125, 458, 1554, 2091, 2126
AlwI	GGATC	6 cut(s)	1175, 1188, 1843, 1907, 1920, 2265
AlwNI	CAGNNNCTG	1 cut(s)	1343
Ama87I	CYCGRG	1 cut(s)	1603
AoxI	GGCC	4 cut(s)	331, 923, 1098, 1504
ApeKI	GCWGC	6 cut(s)	623, 908, 1460, 1490, 1724, 2152
ApoI	RAATTY	2 cut(s)	66, 993
AseI	ATTAAT	1 cut(s)	1113
Asp700I	GAANNNNTTC	3 cut(s)	659, 774, 2280
AspA2I	CCTAGG	1 cut(s)	1184
AspLEI	GCGC	1 cut(s)	700
AspS9I	GGNCC	7 cut(s)	470, 812, 923, 1055, 1372, 1504, 1541
AsuC2I	CCSGG	4 cut(s)	532, 1604, 1605, 1917
AsuHPI	GGTGA	6 cut(s)	359, 421, 570, 1337, 2257, 2285
AsuII	TTCGAA	1 cut(s)	71
AvaI	CYCGRG	1 cut(s)	1603
AvaII	GGWCC	5 cut(s)	470, 812, 1055, 1372, 1541
AvrII	CCTAGG	1 cut(s)	1184
BaeGI	GKGCMC	1 cut(s)	1611
BalI	TGGCCA	1 cut(s)	1100
BamHI	GGATCC	2 cut(s)	1180, 1912
BanI	GGYRCC	2 cut(s)	688, 1608
BanII	GRGCYC	4 cut(s)	425, 538, 1282, 1634
BbrPI	CACGTG	1 cut(s)	695
BbsI	GAAGAC	1 cut(s)	1950
Bbv12I	GWGCWC	2 cut(s)	1282, 1967
BbvI	GCAGC	6 cut(s)	610, 920, 1472, 1477, 1736, 2139
BccI	CCATC	5 cut(s)	46, 587, 776, 1268, 2043
BceAI	ACGGC	3 cut(s)	193, 1287, 1806
BciT130I	CCWGG	1 cut(s)	1394
BciVI	GTATCC	2 cut(s)	735, 2307
BclI	TGATCA	1 cut(s)	106
BcnI	CCSGG	4 cut(s)	532, 1604, 1605, 1917
BcoDI	GTCTC	6 cut(s)	3, 125, 458, 1554, 2091, 2126
BfaI	CTAG	7 cut(s)	539, 678, 797, 1185, 1922, 2142, 2225
BfmI	CTRYAG	3 cut(s)	116, 142, 729
BfrI	CTTAAG	1 cut(s)	847
BfuAI	ACCTGC	1 cut(s)	902
BfuI	GTATCC	2 cut(s)	735, 2307
BisI	GCNGC	6 cut(s)	624, 909, 1461, 1491, 1725, 2153
BlnI	CCTAGG	1 cut(s)	1184
BlsI	GCNGC	6 cut(s)	625, 910, 1462, 1492, 1726, 2154
Bme1390I	CCNGG	5 cut(s)	532, 1394, 1604, 1605, 1917
Bme18I	GGWCC	5 cut(s)	470, 812, 1055, 1372, 1541
BmeT110I	CYCGRG	1 cut(s)	1603
BmgT120I	GGNCC	7 cut(s)	470, 812, 923, 1055, 1372, 1504, 1541
BmiI	GGNNCC	6 cut(s)	690, 1182, 1374, 1610, 1631, 1914
BmrFI	CCNGG	5 cut(s)	532, 1394, 1604, 1605, 1917
BmsI	GCATC	4 cut(s)	390, 1096, 1478, 1781
BpiI	GAAGAC	1 cut(s)	1950
BplI	GAGNNNNNCTC	2 cut(s)	2123, 2155
BpmI	CTGGAG	2 cut(s)	657, 2028
Bpu14I	TTCGAA	1 cut(s)	71
BpuEI	CTTGAG	3 cut(s)	149, 317, 2202
BpuMI	CCSGG	4 cut(s)	532, 1604, 1605, 1917
BsaAI	YACGTR	1 cut(s)	695
BsaBI	GATNNNNATC	1 cut(s)	1980
BsaHI	GRCGYC	1 cut(s)	792
BsaI	GGTCTC	1 cut(s)	2091
BsaXI	ACNNNNNCTCC	8 cut(s)	268, 298, 630, 660, 777, 807, 2188, 2218
Bsc4I	CCNNNNNNNGG	8 cut(s)	205, 734, 830, 1064, 1322, 1618, 2162, 2254
Bse1I	ACTGG	4 cut(s)	139, 472, 2011, 2333
Bse3DI	GCAATG	5 cut(s)	502, 543, 1332, 1967, 2259
Bse8I	GATNNNNATC	1 cut(s)	1980
BseBI	CCWGG	1 cut(s)	1394
BseGI	GGATG	4 cut(s)	1111, 1244, 1438, 2035
BseJI	GATNNNNATC	1 cut(s)	1980
BseLI	CCNNNNNNNGG	8 cut(s)	205, 734, 830, 1064, 1322, 1618, 2162, 2254
BseMI	GCAATG	5 cut(s)	502, 543, 1332, 1967, 2259
BseMII	CTCAG	3 cut(s)	410, 540, 603
BseNI	ACTGG	4 cut(s)	139, 472, 2011, 2333
BseRI	GAGGAG	2 cut(s)	276, 2175
BseSI	GKGCMC	1 cut(s)	1611
BseXI	GCAGC	6 cut(s)	610, 920, 1472, 1477, 1736, 2139
BseYI	CCCAGC	2 cut(s)	481, 887
BsgI	GTGCAG	1 cut(s)	1198
Bsh1236I	CGCG	1 cut(s)	700
BshFI	GGCC	4 cut(s)	333, 925, 1100, 1506
BshNI	GGYRCC	2 cut(s)	688, 1608
BsiHKAI	GWGCWC	2 cut(s)	1282, 1967
BsiHKCI	CYCGRG	1 cut(s)	1603
BsiSI	CCGG	4 cut(s)	531, 981, 1604, 1916
BslFI	GGGAC	3 cut(s)	1358, 1677, 2179
BslI	CCNNNNNNNGG	8 cut(s)	205, 734, 830, 1064, 1322, 1618, 2162, 2254
BsmAI	GTCTC	6 cut(s)	3, 125, 458, 1554, 2091, 2126
BsmFI	GGGAC	3 cut(s)	1358, 1677, 2179
BsmI	GAATGC	1 cut(s)	20
BsnI	GGCC	4 cut(s)	333, 925, 1100, 1506
Bso31I	GGTCTC	1 cut(s)	2091
BsoBI	CYCGRG	1 cut(s)	1603
Bsp119I	TTCGAA	1 cut(s)	71
Bsp1286I	GDGCHC	6 cut(s)	425, 538, 1282, 1611, 1634, 1967
Bsp1407I	TGTACA	1 cut(s)	391
Bsp143I	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
Bsp19I	CCATGG	1 cut(s)	1908
BspACI	CCGC	2 cut(s)	700, 824
BspANI	GGCC	4 cut(s)	333, 925, 1100, 1506
BspCNI	CTCAG	3 cut(s)	411, 539, 602
BspFNI	CGCG	1 cut(s)	700
BspHI	TCATGA	2 cut(s)	103, 2109
BspLI	GGNNCC	6 cut(s)	690, 1182, 1374, 1610, 1631, 1914
BspMI	ACCTGC	1 cut(s)	902
BspPI	GGATC	6 cut(s)	1175, 1188, 1843, 1907, 1920, 2265
BspQI	GCTCTTC	1 cut(s)	1480
BspT104I	TTCGAA	1 cut(s)	71
BspT107I	GGYRCC	2 cut(s)	688, 1608
BspTI	CTTAAG	1 cut(s)	847
BspTNI	GGTCTC	1 cut(s)	2091
BsrDI	GCAATG	5 cut(s)	502, 543, 1332, 1967, 2259
BsrGI	TGTACA	1 cut(s)	391
BsrI	ACTGG	4 cut(s)	139, 472, 2011, 2333
BssMI	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
BssNAI	GTATAC	1 cut(s)	2231
BssNI	GRCGYC	1 cut(s)	792
BssT1I	CCWWGG	4 cut(s)	956, 1101, 1184, 1908
Bst1107I	GTATAC	1 cut(s)	2231
Bst2UI	CCWGG	1 cut(s)	1394
Bst4CI	ACNGT	7 cut(s)	62, 560, 733, 1653, 2002, 2173, 2235
Bst6I	CTCTTC	3 cut(s)	1149, 1480, 1537
BstACI	GRCGYC	1 cut(s)	792
BstAFI	CTTAAG	1 cut(s)	847
BstAPI	GCANNNNNTGC	1 cut(s)	695
BstAUI	TGTACA	1 cut(s)	391
BstBAI	YACGTR	1 cut(s)	695
BstBI	TTCGAA	1 cut(s)	71
BstC8I	GCNNGC	5 cut(s)	149, 906, 1572, 1885, 2126
BstDEI	CTNAG	5 cut(s)	398, 419, 526, 589, 1645
BstDSI	CCRYGG	2 cut(s)	1788, 1908
BstENI	CCTNNNNNAGG	1 cut(s)	732
BstF5I	GGATG	4 cut(s)	1111, 1244, 1438, 2035
BstFNI	CGCG	1 cut(s)	700
BstHHI	GCGC	1 cut(s)	700
BstKTI	GATC	5 cut(s)	109, 1183, 1838, 1915, 2260
BstMAI	GTCTC	6 cut(s)	3, 125, 458, 1554, 2091, 2126
BstMBI	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
BstNI	CCWGG	1 cut(s)	1394
BstNSI	RCATGY	1 cut(s)	856
BstSCI	CCNGG	5 cut(s)	530, 1392, 1602, 1603, 1915
BstSFI	CTRYAG	3 cut(s)	116, 142, 729
BstSLI	GKGCMC	1 cut(s)	1611
BstUI	CGCG	1 cut(s)	700
BstV1I	GCAGC	6 cut(s)	610, 920, 1472, 1477, 1736, 2139
BstV2I	GAAGAC	1 cut(s)	1950
BstX2I	RGATCY	3 cut(s)	1180, 1835, 1912
BstYI	RGATCY	3 cut(s)	1180, 1835, 1912
BstZ17I	GTATAC	1 cut(s)	2231
BsuI	GTATCC	2 cut(s)	735, 2307
BsuRI	GGCC	4 cut(s)	333, 925, 1100, 1506
BtgI	CCRYGG	2 cut(s)	1788, 1908
BtsCI	GGATG	4 cut(s)	1111, 1244, 1438, 2035
BtsIMutI	CAGTG	3 cut(s)	67, 570, 2004
BveI	ACCTGC	1 cut(s)	902
Cac8I	GCNNGC	5 cut(s)	149, 906, 1572, 1885, 2126
CaiI	CAGNNNCTG	1 cut(s)	1343
CciI	TCATGA	2 cut(s)	103, 2109
CfoI	GCGC	1 cut(s)	700
Cfr13I	GGNCC	7 cut(s)	470, 812, 923, 1055, 1372, 1504, 1541
Cfr9I	CCCGGG	1 cut(s)	1603
Csp6I	GTAC	8 cut(s)	280, 392, 1580, 1689, 1741, 1989, 2096, 2214
CspCI	CAANNNNNGTGG	4 cut(s)	1158, 1193, 1852, 1887
CviQI	GTAC	8 cut(s)	280, 392, 1580, 1689, 1741, 1989, 2096, 2214
DdeI	CTNAG	5 cut(s)	398, 419, 526, 589, 1645
DpnI	GATC	5 cut(s)	108, 1182, 1837, 1914, 2259
DpnII	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
DraI	TTTAAA	1 cut(s)	163
DraIII	CACNNNGTG	1 cut(s)	695
DrdI	GACNNNNNNGTC	1 cut(s)	603
DseDI	GACNNNNNNGTC	1 cut(s)	603
EaeI	YGGCCR	1 cut(s)	1098
Eam1104I	CTCTTC	3 cut(s)	1149, 1480, 1537
EarI	CTCTTC	3 cut(s)	1149, 1480, 1537
Ecl136II	GAGCTC	1 cut(s)	1280
Eco130I	CCWWGG	4 cut(s)	956, 1101, 1184, 1908
Eco24I	GRGCYC	4 cut(s)	425, 538, 1282, 1634
Eco31I	GGTCTC	1 cut(s)	2091
Eco47I	GGWCC	5 cut(s)	470, 812, 1055, 1372, 1541
Eco53kI	GAGCTC	1 cut(s)	1280
Eco57I	CTGAAG	4 cut(s)	327, 1851, 1917, 2043
Eco72I	CACGTG	1 cut(s)	695
Eco88I	CYCGRG	1 cut(s)	1603
EcoICRI	GAGCTC	1 cut(s)	1280
EcoNI	CCTNNNNNAGG	1 cut(s)	732
EcoRII	CCWGG	1 cut(s)	1392
EcoT14I	CCWWGG	4 cut(s)	956, 1101, 1184, 1908
EcoT38I	GRGCYC	4 cut(s)	425, 538, 1282, 1634
ErhI	CCWWGG	4 cut(s)	956, 1101, 1184, 1908
FalI	AAGNNNNNCTT	4 cut(s)	391, 423, 1477, 1509
FaqI	GGGAC	3 cut(s)	1358, 1677, 2179
FbaI	TGATCA	1 cut(s)	106
FblI	GTMKAC	2 cut(s)	2175, 2230
Fnu4HI	GCNGC	6 cut(s)	624, 909, 1461, 1491, 1725, 2153
FokI	GGATG	4 cut(s)	1118, 1231, 1445, 2022
FriOI	GRGCYC	4 cut(s)	425, 538, 1282, 1634
Fsp4HI	GCNGC	6 cut(s)	624, 909, 1461, 1491, 1725, 2153
FspBI	CTAG	7 cut(s)	539, 678, 797, 1185, 1922, 2142, 2225
GlaI	GCGC	1 cut(s)	699
GluI	GCNGC	6 cut(s)	624, 909, 1461, 1491, 1725, 2153
GsaI	CCCAGC	2 cut(s)	485, 891
GsuI	CTGGAG	2 cut(s)	657, 2028
HaeIII	GGCC	4 cut(s)	333, 925, 1100, 1506
HapII	CCGG	4 cut(s)	531, 981, 1604, 1916
HhaI	GCGC	1 cut(s)	700
Hin1I	GRCGYC	1 cut(s)	792
Hin6I	GCGC	1 cut(s)	698
HinP1I	GCGC	1 cut(s)	698
HindIII	AAGCTT	1 cut(s)	149
HinfI	GANTC	8 cut(s)	11, 98, 374, 587, 1230, 1527, 1756, 2299
HpaII	CCGG	4 cut(s)	531, 981, 1604, 1916
HphI	GGTGA	6 cut(s)	359, 421, 570, 1337, 2257, 2285
Hpy166II	GTNNAC	5 cut(s)	282, 790, 2176, 2196, 2231
Hpy188I	TCNGA	9 cut(s)	231, 420, 592, 812, 1225, 1286, 1478, 1840, 2049
Hpy188III	TCNNGA	9 cut(s)	104, 128, 779, 1053, 1536, 1935, 2110, 2219, 2311
Hpy8I	GTNNAC	5 cut(s)	282, 790, 2176, 2196, 2231
Hpy99I	CGWCG	1 cut(s)	885
HpyCH4III	ACNGT	7 cut(s)	62, 560, 733, 1653, 2002, 2173, 2235
HpyCH4IV	ACGT	6 cut(s)	168, 278, 337, 694, 792, 2115
HpyF3I	CTNAG	5 cut(s)	398, 419, 526, 589, 1645
HpySE526I	ACGT	6 cut(s)	168, 278, 337, 694, 792, 2115
Hsp92I	GRCGYC	1 cut(s)	792
HspAI	GCGC	1 cut(s)	698
Ksp22I	TGATCA	1 cut(s)	106
Kzo9I	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
LguI	GCTCTTC	1 cut(s)	1480
LmnI	GCTCC	6 cut(s)	232, 1025, 1622, 1629, 1880, 2209
Lsp1109I	GCAGC	6 cut(s)	610, 920, 1472, 1477, 1736, 2139
LweI	GCATC	4 cut(s)	390, 1096, 1478, 1781
MaeI	CTAG	7 cut(s)	539, 678, 797, 1185, 1922, 2142, 2225
MaeII	ACGT	6 cut(s)	168, 278, 337, 694, 792, 2115
MaeIII	GTNAC	9 cut(s)	300, 338, 838, 1074, 1325, 1381, 2002, 2062, 2242
MalI	GATC	5 cut(s)	108, 1182, 1837, 1914, 2259
MboI	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
MboII	GAAGA	7 cut(s)	33, 969, 1166, 1497, 1524, 1923, 1955
MfeI	CAATTG	3 cut(s)	89, 762, 1119
MflI	RGATCY	3 cut(s)	1180, 1835, 1912
MhlI	GDGCHC	6 cut(s)	425, 538, 1282, 1611, 1634, 1967
MlsI	TGGCCA	1 cut(s)	1100
MluNI	TGGCCA	1 cut(s)	1100
MlyI	GAGTC	3 cut(s)	5, 581, 1750
MmeI	TCCRAC	1 cut(s)	790
Mox20I	TGGCCA	1 cut(s)	1100
MroXI	GAANNNNTTC	3 cut(s)	659, 774, 2280
MscI	TGGCCA	1 cut(s)	1100
MseI	TTAA	4 cut(s)	162, 848, 1113, 1734
Msp20I	TGGCCA	1 cut(s)	1100
MspCI	CTTAAG	1 cut(s)	847
MspI	CCGG	4 cut(s)	531, 981, 1604, 1916
MspR9I	CCNGG	5 cut(s)	532, 1394, 1604, 1605, 1917
MunI	CAATTG	3 cut(s)	89, 762, 1119
Mva1269I	GAATGC	1 cut(s)	20
MvaI	CCWGG	1 cut(s)	1394
MvnI	CGCG	1 cut(s)	700
NciI	CCSGG	4 cut(s)	532, 1604, 1605, 1917
NcoI	CCATGG	1 cut(s)	1908
NdeII	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
NlaIV	GGNNCC	6 cut(s)	690, 1182, 1374, 1610, 1631, 1914
NmuCI	GTSAC	4 cut(s)	838, 1325, 2002, 2242
NspI	RCATGY	1 cut(s)	856
NspV	TTCGAA	1 cut(s)	71
PagI	TCATGA	2 cut(s)	103, 2109
PciSI	GCTCTTC	1 cut(s)	1480
PcsI	WCGNNNNNNNCGW	1 cut(s)	816
PctI	GAATGC	1 cut(s)	20
PdmI	GAANNNNTTC	3 cut(s)	659, 774, 2280
PfeI	GAWTC	5 cut(s)	98, 374, 1230, 1527, 2299
PflMI	CCANNNNNTGG	4 cut(s)	1064, 1322, 2162, 2254
PkrI	GCNGC	6 cut(s)	625, 910, 1462, 1492, 1726, 2154
PleI	GAGTC	3 cut(s)	5, 581, 1750
PmaCI	CACGTG	1 cut(s)	695
PmlI	CACGTG	1 cut(s)	695
PpsI	GAGTC	3 cut(s)	5, 581, 1750
Ppu21I	YACGTR	1 cut(s)	695
PshBI	ATTAAT	1 cut(s)	1113
PsiI	TTATAA	1 cut(s)	411
Psp124BI	GAGCTC	1 cut(s)	1282
Psp6I	CCWGG	1 cut(s)	1392
PspCI	CACGTG	1 cut(s)	695
PspFI	CCCAGC	2 cut(s)	481, 887
PspGI	CCWGG	1 cut(s)	1392
PspN4I	GGNNCC	6 cut(s)	690, 1182, 1374, 1610, 1631, 1914
PspPI	GGNCC	7 cut(s)	470, 812, 923, 1055, 1372, 1504, 1541
PstNI	CAGNNNCTG	1 cut(s)	1343
PsuI	RGATCY	3 cut(s)	1180, 1835, 1912
RsaI	GTAC	8 cut(s)	281, 393, 1581, 1690, 1742, 1990, 2097, 2215
RsaNI	GTAC	8 cut(s)	280, 392, 1580, 1689, 1741, 1989, 2096, 2214
SacI	GAGCTC	1 cut(s)	1282
SapI	GCTCTTC	1 cut(s)	1480
SaqAI	TTAA	4 cut(s)	162, 848, 1113, 1734
SatI	GCNGC	6 cut(s)	624, 909, 1461, 1491, 1725, 2153
Sau3AI	GATC	5 cut(s)	106, 1180, 1835, 1912, 2257
Sau96I	GGNCC	7 cut(s)	470, 812, 923, 1055, 1372, 1504, 1541
SchI	GAGTC	3 cut(s)	5, 581, 1750
ScrFI	CCNGG	5 cut(s)	532, 1394, 1604, 1605, 1917
SduI	GDGCHC	6 cut(s)	425, 538, 1282, 1611, 1634, 1967
SfaNI	GCATC	4 cut(s)	390, 1096, 1478, 1781
SfcI	CTRYAG	3 cut(s)	116, 142, 729
SfuI	TTCGAA	1 cut(s)	71
SinI	GGWCC	5 cut(s)	470, 812, 1055, 1372, 1541
SmaI	CCCGGG	1 cut(s)	1605
SmlI	CTYRAG	4 cut(s)	128, 296, 847, 2217
SmoI	CTYRAG	4 cut(s)	128, 296, 847, 2217
SsiI	CCGC	2 cut(s)	700, 824
SspI	AATATT	1 cut(s)	439
SspMI	CTAG	7 cut(s)	539, 678, 797, 1185, 1922, 2142, 2225
SstI	GAGCTC	1 cut(s)	1282
StyD4I	CCNGG	5 cut(s)	530, 1392, 1602, 1603, 1915
StyI	CCWWGG	4 cut(s)	956, 1101, 1184, 1908
TaaI	ACNGT	7 cut(s)	62, 560, 733, 1653, 2002, 2173, 2235
TaiI	ACGT	6 cut(s)	171, 281, 340, 697, 795, 2118
TaqI	TCGA	5 cut(s)	71, 778, 880, 919, 1535
TatI	WGTACW	4 cut(s)	391, 1579, 1740, 1988
TfiI	GAWTC	5 cut(s)	98, 374, 1230, 1527, 2299
Tru1I	TTAA	4 cut(s)	162, 848, 1113, 1734
Tru9I	TTAA	4 cut(s)	162, 848, 1113, 1734
TscAI	CASTG	3 cut(s)	67, 570, 2011
TseFI	GTSAC	4 cut(s)	838, 1325, 2002, 2242
TseI	GCWGC	6 cut(s)	623, 908, 1460, 1490, 1724, 2152
Tsp45I	GTSAC	4 cut(s)	838, 1325, 2002, 2242
TspMI	CCCGGG	1 cut(s)	1603
TspRI	CASTG	3 cut(s)	67, 570, 2011
Van91I	CCANNNNNTGG	4 cut(s)	1064, 1322, 2162, 2254
Vha464I	CTTAAG	1 cut(s)	847
VpaK11BI	GGWCC	5 cut(s)	470, 812, 1055, 1372, 1541
VspI	ATTAAT	1 cut(s)	1113
XagI	CCTNNNNNAGG	1 cut(s)	732
XapI	RAATTY	2 cut(s)	66, 993
XceI	RCATGY	1 cut(s)	856
XcmI	CCANNNNNNNNNTGG	1 cut(s)	341
XmaI	CCCGGG	1 cut(s)	1603
XmaJI	CCTAGG	1 cut(s)	1184
XmiI	GTMKAC	2 cut(s)	2175, 2230
XmnI	GAANNNNTTC	3 cut(s)	659, 774, 2280
XspI	CTAG	7 cut(s)	539, 678, 797, 1185, 1922, 2142, 2225
ZraI	GACGTC	1 cut(s)	793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.