Rroxscaffold_1G00073760

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006269
Physical Location & Seq
Reverse (-)
94490327 .. 94491210
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073760.1

Sequence Viewer

Length: 636 bp
ATGGTAGGAGACTCAGTTGCATTCAATGGAGGCTCTCCATCTTCACGCCTCAACCTAAACAGTGAAGTTTCGAAGGCTCTTGCAAAGCAATTGGAGGATTCTCATGATCAACTTCTACAGAGAAATCTTGATACCGGAAGCTACAGCAAGCTTCACAGCTTTAAACACATTGCAAACGGCTTTGCTGTCCGTACAACTCCTTCTCAGGTTGAAAAGCTCAAATATGCTCCAGGAGTAAAGCTGGTTGAGAGAGATAGAGGAGCAAAGTTGATGACAACATACACTCCTCGCCTCCTTGAGTTACCTGAAGGAGTATGGACACAAGAAGGAGGCCAACGTAATGCTGGTGAAGGAATTGTGATTGGTTTTGTGGATAGTGGCATCAACCCTGCACATCTAAGTTTTGCTTATAATCCTCTGAGCCCTTTCACCTCTCCAATATTTCTCATTTTTCGGGTGCTTGTGAGACTGGTCCTCGATTCCCAGCAACTTCATGCTATGATAAAATTGTTTCAGCCAAGTTTTTCTCAGCTGGGGCTCGAGCCATTGCTACTCTCAACACTTCAGTGGATTTTCTTTCACCCTTTGACGCAGATGGACATGGCAGTTAGCTTGAGTGACTTTAAATGCTTTTGA

Protein Analysis

211

Amino Acids

23.42

Weight (kDa)

7.9

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 24 - 90 1.3e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AcuI CTGAAG 2 cut(s) 327, 548
AfaI GTAC 1 cut(s) 193
AgsI TTSAA 2 cut(s) 25, 212
AjnI CCWGG 1 cut(s) 229
AleI CACNNNNGTG 1 cut(s) 565
AluBI AGCT 7 cut(s) 141, 151, 159, 217, 241, 532, 612
AluI AGCT 7 cut(s) 141, 151, 159, 217, 241, 532, 612
Alw26I GTCTC 2 cut(s) 3, 460
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 3 cut(s) 359, 421, 572
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 1 cut(s) 472
BanII GRGCYC 2 cut(s) 425, 540
BarI GAAGNNNNNNTAC 2 cut(s) 184, 216
BccI CCATC 2 cut(s) 46, 589
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 231
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 2 cut(s) 3, 460
BfmI CTRYAG 2 cut(s) 116, 142
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 472
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 317, 634
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 3 cut(s) 30, 268, 298
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 2 cut(s) 168, 545
BseBI CCWGG 1 cut(s) 231
BseMI GCAATG 2 cut(s) 168, 545
BseMII CTCAG 4 cut(s) 27, 218, 410, 542
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 2 cut(s) 273, 276
BseYI CCCAGC 2 cut(s) 483, 532
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 1 cut(s) 135
BsmAI GTCTC 2 cut(s) 3, 460
BsmI GAATGC 1 cut(s) 20
BsnI GGCC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 425, 540
Bsp143I GATC 1 cut(s) 106
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 4 cut(s) 26, 217, 411, 541
BspHI TCATGA 1 cut(s) 103
BspT104I TTCGAA 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 168, 545
BsrI ACTGG 1 cut(s) 474
BssMI GATC 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 62
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 5 cut(s) 13, 204, 398, 419, 528
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 2 cut(s) 3, 460
BstMBI GATC 1 cut(s) 106
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 2 cut(s) 116, 142
BsuRI GGCC 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 67, 572
Cac8I GCNNGC 1 cut(s) 149
CciI TCATGA 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 472
CseI GACGC 1 cut(s) 598
Csp6I GTAC 1 cut(s) 192
CviAII CATG 3 cut(s) 104, 494, 601
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 5 cut(s) 13, 204, 398, 419, 528
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
DraI TTTAAA 2 cut(s) 163, 625
Eco24I GRGCYC 2 cut(s) 425, 540
Eco47I GGWCC 1 cut(s) 472
Eco57I CTGAAG 2 cut(s) 327, 548
Eco88I CYCGRG 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 229
EcoT38I GRGCYC 2 cut(s) 425, 540
FaeI CATG 3 cut(s) 107, 497, 604
FaiI YATR 8 cut(s) 105, 225, 280, 316, 411, 495, 500, 602
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FatI CATG 3 cut(s) 103, 493, 600
FbaI TGATCA 1 cut(s) 106
FriOI GRGCYC 2 cut(s) 425, 540
GsaI CCCAGC 2 cut(s) 487, 536
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 1 cut(s) 333
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 598
Hin1II CATG 3 cut(s) 107, 497, 604
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 3 cut(s) 11, 98, 479
HpaII CCGG 1 cut(s) 135
HphI GGTGA 3 cut(s) 359, 421, 572
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 2 cut(s) 104, 128
HpyAV CCTTC 5 cut(s) 67, 210, 302, 320, 344
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 4 cut(s) 20, 83, 173, 392
HpyF3I CTNAG 5 cut(s) 13, 204, 398, 419, 528
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 3 cut(s) 107, 497, 604
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 2 cut(s) 232, 260
LweI GCATC 1 cut(s) 390
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 2 cut(s) 300, 617
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 1 cut(s) 33
MfeI CAATTG 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 425, 540
MluCI AATT 3 cut(s) 89, 354, 506
MlyI GAGTC 1 cut(s) 5
MseI TTAA 2 cut(s) 162, 624
MslI CAYNNNNRTG 1 cut(s) 565
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 20
MvaI CCWGG 1 cut(s) 231
NdeII GATC 1 cut(s) 106
NlaIII CATG 3 cut(s) 107, 497, 604
NmuCI GTSAC 1 cut(s) 617
NspV TTCGAA 1 cut(s) 71
OliI CACNNNNGTG 1 cut(s) 565
PaeR7I CTCGAG 1 cut(s) 539
PagI TCATGA 1 cut(s) 103
PctI GAATGC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 98, 479
PfoI TCCNGGA 1 cut(s) 229
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PsiI TTATAA 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 2 cut(s) 483, 532
PspGI CCWGG 1 cut(s) 229
PspPI GGNCC 1 cut(s) 472
PspXI VCTCGAGB 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 565
SaqAI TTAA 2 cut(s) 162, 624
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 472
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 425, 540
SfaNI GCATC 1 cut(s) 390
SfcI CTRYAG 2 cut(s) 116, 142
Sfr274I CTCGAG 1 cut(s) 539
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 472
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 565
SmlI CTYRAG 3 cut(s) 296, 539, 613
SmoI CTYRAG 3 cut(s) 296, 539, 613
Sse9I AATT 3 cut(s) 89, 354, 506
SspI AATATT 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 71, 477, 540
TasI AATT 3 cut(s) 89, 354, 506
TfiI GAWTC 2 cut(s) 98, 479
Tru1I TTAA 2 cut(s) 162, 624
Tru9I TTAA 2 cut(s) 162, 624
TscAI CASTG 2 cut(s) 67, 572
TseFI GTSAC 1 cut(s) 617
Tsp45I GTSAC 1 cut(s) 617
TspDTI ATGAA 1 cut(s) 482
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 2 cut(s) 67, 572
VpaK11BI GGWCC 1 cut(s) 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XhoI CTCGAG 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.