Rh5AG017100

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006269
Physical Location & Seq
Forward (+)
1226136 .. 1229828
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG017100.1

Sequence Viewer

Length: 1596 bp
ATGGAAGAAGACTCGGTTGCAATCAATGGAGGCTCTCCATCTTCACTCCTCAACCTAAATAGTGAAGTTTCGAAGGCTCATGTAAAGCAATTGGAGGATTCTCATGATCAACTTCTACAGAGCAATCTTGAGACCGGAAGCTACAGAAATCTTTACAGCTTTAAACACGTTGCAAACGGGTTTGCTGTCCATACAACCCCTTCTCAGAGGAGCAAAGTTGATGACAAATTGACAACGTACACTCCTCGCTTCCTCGAGTTACCTGAAGGAGTATGGACACAAGAAGGAGGCCAACGTAATGCTGGTGAAGGAATTGTGATTGGTTTTGTGGATAGTGGCATCAATCCTGCACATCTAAGTTTTGCTTATAATCCTTTGAGCCCTTTCACCTCCAATATTTCTCATTTTTCGGGCGCTTGTGAGACTGTTCCTCTATTCCCAGCAACTTCATGCAATGGTAAAATTGTTTCAGCTAAGTTTTTCTCAGCTGGGGCTCGAGCCATTGCTACTCTCAACGCTTCGGTGGATTTTCTTTCACCTTCGACGCAGATGGACATGGCAGTTGCTGCTGGAAATGTTGGAGTTCCTGTGGTTGTGAATGGCTTCAGTTATGGACGAGCTAGTAGAATAGCACCACGTGCGCGGATTGCTGTATATAAAGCTGTGTATCCTACAGTAGGAACTTTGGCAGATGTAGTTTCTGCAATTGATGAAGCAATTCTAGATGGAGTGGATGTCCTAGCACTCTCTGTCGGACCAGATGAACCGCCATTGGGTAAAGTGACCTTCTTAAGCATGTTTGATGTGTTCATGCTGTATGCTCGACGAGCTGGGGTCTTCGTGGTGCAAGCAGCAGGTAATCGAGGGCCAGACCCTTCAAGTGTAGTGTCTTACAGCCCTTGGGCAGTAGGTGTAGCTTCTTCCGGCACAGATAGAATTTATCCTTCTTCTATTGTTCTTGGAAATGGAGCAAGAATTGAAGGACCAACTTTTGGATATAGGTTACTTCAACATAGGTTGGTTTTGGCCAAGGATGCAATTAATCCAATTGGGTCATTCCCAAGAACCCCACCCTATGTTGAAGAGTGCCAATTTCCACAAGCATTGGATCCTAGGTTAGTTATAGGTAGTATTGTCATCTGCACATTCTCGGAGGGATTCTATAACAGGACATCCACCCTTTTTGCCATCATCAACACGGCAAGAGCTCTCGGATTTATGGGTTTTGTTCTTGTTGCAAATCCAGCCTATGGTGACTTCATTGCAGAGCCTGTTCCTTTTGCTGTTTCTGGCATTTTGGTCCCAAATGTAACCAATTCCCAGGTTATATTACAATACTATGAACAACAAACATTCAAGGATGAGAGAGGATTTCCTACAAGACTTGCAGCGAGAGCATCAATCGGAGAAGGAAGAGCTGCTTCTTTTACGGGCCAAGCACCTATTGTTAGCAGATTCTCTTCGAGAGGACCAGATTTCATTGACAGAGACAGGAAGCCTGCTGATGTACTAAAGCCTGATATTCTTGCCCCGGGGCACCAAATCTGGGGAGCTTGGAGCTCTATTAGTGCCTTTAGAACCCTCATTAGTGGGTGA

Protein Analysis

531

Amino Acids

56.82

Weight (kDa)

6.27

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 102 - 514 3.7e-12 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 1536
AccB7I CCANNNNNTGG 2 cut(s) 992, 1250
AccII CGCG 1 cut(s) 643
AciI CCGC 2 cut(s) 643, 767
AclWI GGATC 2 cut(s) 1103, 1116
AcoI YGGCCR 1 cut(s) 1026
AcsI RAATTY 1 cut(s) 936
AcuI CTGAAG 2 cut(s) 285, 589
AcvI CACGTG 1 cut(s) 638
AdeI CACNNNGTG 1 cut(s) 638
AfaI GTAC 2 cut(s) 239, 1509
AfiI CCNNNNNNNGG 5 cut(s) 677, 773, 992, 1250, 1546
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 5 cut(s) 879, 980, 1010, 1082, 1357
AjnI CCWGG 1 cut(s) 1320
Alw21I GWGCWC 2 cut(s) 1210, 1562
Alw26I GTCTC 3 cut(s) 125, 416, 1482
AlwI GGATC 2 cut(s) 1103, 1116
AlwNI CAGNNNCTG 2 cut(s) 566, 1271
Ama87I CYCGRG 3 cut(s) 254, 495, 1531
AoxI GGCC 4 cut(s) 289, 866, 1026, 1432
ApeKI GCWGC 4 cut(s) 566, 851, 1388, 1418
ApoI RAATTY 1 cut(s) 936
AseI ATTAAT 1 cut(s) 1041
Asp700I GAANNNNTTC 2 cut(s) 602, 717
AspA2I CCTAGG 1 cut(s) 1112
AspLEI GCGC 2 cut(s) 416, 643
AspS9I GGNCC 6 cut(s) 755, 866, 983, 1300, 1432, 1469
AsuC2I CCSGG 2 cut(s) 1532, 1533
AsuHPI GGTGA 4 cut(s) 317, 379, 528, 1265
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 3 cut(s) 254, 495, 1531
AvaII GGWCC 4 cut(s) 755, 983, 1300, 1469
AvrII CCTAGG 1 cut(s) 1112
BaeGI GKGCMC 1 cut(s) 1539
BalI TGGCCA 1 cut(s) 1028
BamHI GGATCC 1 cut(s) 1108
BanI GGYRCC 1 cut(s) 1536
BanII GRGCYC 4 cut(s) 383, 496, 1210, 1562
BbrPI CACGTG 1 cut(s) 638
BbsI GAAGAC 2 cut(s) 15, 829
Bbv12I GWGCWC 2 cut(s) 1210, 1562
BbvI GCAGC 4 cut(s) 553, 863, 1400, 1405
BccI CCATC 4 cut(s) 46, 544, 719, 1196
BceAI ACGGC 1 cut(s) 1215
BciT130I CCWGG 1 cut(s) 1322
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 2 cut(s) 1532, 1533
BcoDI GTCTC 3 cut(s) 125, 416, 1482
BfaI CTAG 4 cut(s) 621, 722, 740, 1113
BfmI CTRYAG 3 cut(s) 116, 142, 672
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 790
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 678
BisI GCNGC 4 cut(s) 567, 852, 1389, 1419
BlnI CCTAGG 1 cut(s) 1112
BlsI GCNGC 4 cut(s) 568, 853, 1390, 1420
Bme1390I CCNGG 3 cut(s) 1322, 1532, 1533
Bme18I GGWCC 4 cut(s) 755, 983, 1300, 1469
BmeT110I CYCGRG 3 cut(s) 254, 495, 1531
BmgT120I GGNCC 6 cut(s) 755, 866, 983, 1300, 1432, 1469
BmiI GGNNCC 3 cut(s) 1110, 1302, 1538
BmrFI CCNGG 3 cut(s) 1322, 1532, 1533
BmsI GCATC 3 cut(s) 348, 1024, 1406
BpiI GAAGAC 2 cut(s) 15, 829
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 2 cut(s) 1532, 1533
BsaAI YACGTR 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 7 cut(s) 899, 1029, 1112, 1320, 1530, 1531, 1532
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 6 cut(s) 226, 256, 573, 603, 720, 750
Bsc4I CCNNNNNNNGG 5 cut(s) 677, 773, 992, 1250, 1546
Bse3DI GCAATG 3 cut(s) 460, 501, 1260
BseBI CCWGG 1 cut(s) 1322
BseDI CCNNGG 7 cut(s) 899, 1029, 1112, 1320, 1530, 1531, 1532
BseGI GGATG 4 cut(s) 739, 1039, 1172, 1366
BseLI CCNNNNNNNGG 5 cut(s) 677, 773, 992, 1250, 1546
BseMI GCAATG 3 cut(s) 460, 501, 1260
BseMII CTCAG 2 cut(s) 218, 498
BseRI GAGGAG 3 cut(s) 38, 223, 234
BseSI GKGCMC 1 cut(s) 1539
BseXI GCAGC 4 cut(s) 553, 863, 1400, 1405
BseYI CCCAGC 3 cut(s) 439, 488, 830
BsgI GTGCAG 2 cut(s) 333, 1126
Bsh1236I CGCG 1 cut(s) 643
BshFI GGCC 4 cut(s) 291, 868, 1028, 1434
BshNI GGYRCC 1 cut(s) 1536
BsiHKAI GWGCWC 2 cut(s) 1210, 1562
BsiHKCI CYCGRG 3 cut(s) 254, 495, 1531
BsiSI CCGG 3 cut(s) 135, 924, 1532
BslFI GGGAC 1 cut(s) 1286
BslI CCNNNNNNNGG 5 cut(s) 677, 773, 992, 1250, 1546
BsmAI GTCTC 3 cut(s) 125, 416, 1482
BsmFI GGGAC 1 cut(s) 1286
BsnI GGCC 4 cut(s) 291, 868, 1028, 1434
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 3 cut(s) 254, 495, 1531
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 5 cut(s) 383, 496, 1210, 1539, 1562
Bsp143I GATC 2 cut(s) 106, 1108
BspACI CCGC 2 cut(s) 643, 767
BspANI GGCC 4 cut(s) 291, 868, 1028, 1434
BspCNI CTCAG 2 cut(s) 217, 497
BspFNI CGCG 1 cut(s) 643
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 1110, 1302, 1538
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 2 cut(s) 1103, 1116
BspQI GCTCTTC 1 cut(s) 1408
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 1536
BspTI CTTAAG 1 cut(s) 790
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 3 cut(s) 460, 501, 1260
BssECI CCNNGG 7 cut(s) 899, 1029, 1112, 1320, 1530, 1531, 1532
BssMI GATC 2 cut(s) 106, 1108
BssT1I CCWWGG 3 cut(s) 899, 1029, 1112
Bst2UI CCWGG 1 cut(s) 1322
Bst4CI ACNGT 2 cut(s) 427, 676
Bst6I CTCTTC 3 cut(s) 1077, 1408, 1465
BstAFI CTTAAG 1 cut(s) 790
BstAPI GCANNNNNTGC 2 cut(s) 566, 638
BstBAI YACGTR 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 849, 1500
BstDEI CTNAG 4 cut(s) 204, 356, 474, 484
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 4 cut(s) 739, 1039, 1172, 1366
BstFNI CGCG 1 cut(s) 643
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 2 cut(s) 416, 643
BstKTI GATC 2 cut(s) 109, 1111
BstMAI GTCTC 3 cut(s) 125, 416, 1482
BstMBI GATC 2 cut(s) 106, 1108
BstMWI GCNNNNNNNGC 7 cut(s) 566, 638, 647, 827, 1034, 1244, 1394
BstNI CCWGG 1 cut(s) 1322
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 3 cut(s) 1320, 1530, 1531
BstSFI CTRYAG 3 cut(s) 116, 142, 672
BstSLI GKGCMC 1 cut(s) 1539
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 4 cut(s) 553, 863, 1400, 1405
BstV2I GAAGAC 2 cut(s) 15, 829
BstX2I RGATCY 1 cut(s) 1108
BstYI RGATCY 1 cut(s) 1108
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 4 cut(s) 291, 868, 1028, 1434
BtsCI GGATG 4 cut(s) 739, 1039, 1172, 1366
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 849, 1500
CaiI CAGNNNCTG 2 cut(s) 566, 1271
CciI TCATGA 1 cut(s) 103
CfoI GCGC 2 cut(s) 416, 643
Cfr13I GGNCC 6 cut(s) 755, 866, 983, 1300, 1432, 1469
Cfr9I CCCGGG 1 cut(s) 1531
CseI GACGC 1 cut(s) 553
Csp6I GTAC 2 cut(s) 238, 1508
CspCI CAANNNNNGTGG 4 cut(s) 1086, 1121, 1165, 1200
CviAII CATG 6 cut(s) 80, 104, 450, 556, 796, 811
CviQI GTAC 2 cut(s) 238, 1508
DdeI CTNAG 4 cut(s) 204, 356, 474, 484
DpnI GATC 2 cut(s) 108, 1110
DpnII GATC 2 cut(s) 106, 1108
DraI TTTAAA 1 cut(s) 163
DraIII CACNNNGTG 1 cut(s) 638
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 3 cut(s) 1077, 1408, 1465
EarI CTCTTC 3 cut(s) 1077, 1408, 1465
Ecl136II GAGCTC 2 cut(s) 1208, 1560
Eco130I CCWWGG 3 cut(s) 899, 1029, 1112
Eco24I GRGCYC 4 cut(s) 383, 496, 1210, 1562
Eco31I GGTCTC 1 cut(s) 125
Eco47I GGWCC 4 cut(s) 755, 983, 1300, 1469
Eco53kI GAGCTC 2 cut(s) 1208, 1560
Eco57I CTGAAG 2 cut(s) 285, 589
Eco72I CACGTG 1 cut(s) 638
Eco88I CYCGRG 3 cut(s) 254, 495, 1531
EcoICRI GAGCTC 2 cut(s) 1208, 1560
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 1320
EcoT14I CCWWGG 3 cut(s) 899, 1029, 1112
EcoT38I GRGCYC 4 cut(s) 383, 496, 1210, 1562
ErhI CCWWGG 3 cut(s) 899, 1029, 1112
FaeI CATG 6 cut(s) 83, 107, 453, 559, 799, 814
FalI AAGNNNNNCTT 4 cut(s) 349, 381, 1405, 1437
FaqI GGGAC 1 cut(s) 1286
FatI CATG 6 cut(s) 79, 103, 449, 555, 795, 810
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 4 cut(s) 567, 852, 1389, 1419
FokI GGATG 4 cut(s) 746, 1046, 1159, 1373
FriOI GRGCYC 4 cut(s) 383, 496, 1210, 1562
Fsp4HI GCNGC 4 cut(s) 567, 852, 1389, 1419
FspBI CTAG 4 cut(s) 621, 722, 740, 1113
GlaI GCGC 2 cut(s) 415, 642
GluI GCNGC 4 cut(s) 567, 852, 1389, 1419
GsaI CCCAGC 3 cut(s) 443, 492, 834
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 4 cut(s) 291, 868, 1028, 1434
HapII CCGG 3 cut(s) 135, 924, 1532
HgaI GACGC 1 cut(s) 553
HhaI GCGC 2 cut(s) 416, 643
Hin1II CATG 6 cut(s) 83, 107, 453, 559, 799, 814
Hin6I GCGC 2 cut(s) 414, 641
HinP1I GCGC 2 cut(s) 414, 641
HinfI GANTC 4 cut(s) 11, 98, 1158, 1455
HpaII CCGG 3 cut(s) 135, 924, 1532
HphI GGTGA 4 cut(s) 317, 379, 528, 1265
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 5 cut(s) 207, 755, 1153, 1214, 1406
Hpy188III TCNNGA 4 cut(s) 104, 128, 722, 1464
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 2 cut(s) 547, 828
HpyCH4III ACNGT 2 cut(s) 427, 676
HpyCH4IV ACGT 4 cut(s) 168, 236, 295, 637
HpyF10VI GCNNNNNNNGC 7 cut(s) 566, 638, 647, 827, 1034, 1244, 1394
HpyF3I CTNAG 4 cut(s) 204, 356, 474, 484
HpySE526I ACGT 4 cut(s) 168, 236, 295, 637
Hsp92II CATG 6 cut(s) 83, 107, 453, 559, 799, 814
HspAI GCGC 2 cut(s) 414, 641
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 2 cut(s) 106, 1108
LguI GCTCTTC 1 cut(s) 1408
LmnI GCTCC 4 cut(s) 210, 968, 1550, 1557
Lsp1109I GCAGC 4 cut(s) 553, 863, 1400, 1405
LweI GCATC 3 cut(s) 348, 1024, 1406
MaeI CTAG 4 cut(s) 621, 722, 740, 1113
MaeII ACGT 4 cut(s) 168, 236, 295, 637
MaeIII GTNAC 5 cut(s) 258, 781, 1002, 1253, 1309
MalI GATC 2 cut(s) 108, 1110
MboI GATC 2 cut(s) 106, 1108
MboII GAAGA 9 cut(s) 17, 20, 33, 829, 912, 939, 1094, 1425, 1452
MfeI CAATTG 3 cut(s) 89, 705, 1047
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 5 cut(s) 383, 496, 1210, 1539, 1562
MlsI TGGCCA 1 cut(s) 1028
MluNI TGGCCA 1 cut(s) 1028
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 559, 733
Mox20I TGGCCA 1 cut(s) 1028
MroXI GAANNNNTTC 2 cut(s) 602, 717
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 3 cut(s) 162, 791, 1041
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 3 cut(s) 135, 924, 1532
MspR9I CCNGG 3 cut(s) 1322, 1532, 1533
MunI CAATTG 3 cut(s) 89, 705, 1047
MvaI CCWGG 1 cut(s) 1322
MvnI CGCG 1 cut(s) 643
MwoI GCNNNNNNNGC 7 cut(s) 566, 638, 647, 827, 1034, 1244, 1394
NciI CCSGG 2 cut(s) 1532, 1533
NdeII GATC 2 cut(s) 106, 1108
NlaIII CATG 6 cut(s) 83, 107, 453, 559, 799, 814
NlaIV GGNNCC 3 cut(s) 1110, 1302, 1538
NmuCI GTSAC 2 cut(s) 781, 1253
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 71
PaeR7I CTCGAG 2 cut(s) 254, 495
PagI TCATGA 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 1408
PdmI GAANNNNTTC 2 cut(s) 602, 717
PfeI GAWTC 3 cut(s) 98, 1158, 1455
PflMI CCANNNNNTGG 2 cut(s) 992, 1250
PkrI GCNGC 4 cut(s) 568, 853, 1390, 1420
PleI GAGTC 1 cut(s) 5
PmaCI CACGTG 1 cut(s) 638
PmlI CACGTG 1 cut(s) 638
PpsI GAGTC 1 cut(s) 5
Ppu21I YACGTR 1 cut(s) 638
PshBI ATTAAT 1 cut(s) 1041
PsiI TTATAA 1 cut(s) 369
Psp124BI GAGCTC 2 cut(s) 1210, 1562
Psp6I CCWGG 1 cut(s) 1320
PspCI CACGTG 1 cut(s) 638
PspFI CCCAGC 3 cut(s) 439, 488, 830
PspGI CCWGG 1 cut(s) 1320
PspN4I GGNNCC 3 cut(s) 1110, 1302, 1538
PspPI GGNCC 6 cut(s) 755, 866, 983, 1300, 1432, 1469
PspXI VCTCGAGB 2 cut(s) 254, 495
PstNI CAGNNNCTG 2 cut(s) 566, 1271
PsuI RGATCY 1 cut(s) 1108
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 2 cut(s) 239, 1509
RsaNI GTAC 2 cut(s) 238, 1508
SacI GAGCTC 2 cut(s) 1210, 1562
SapI GCTCTTC 1 cut(s) 1408
SaqAI TTAA 3 cut(s) 162, 791, 1041
SatI GCNGC 4 cut(s) 567, 852, 1389, 1419
Sau3AI GATC 2 cut(s) 106, 1108
Sau96I GGNCC 6 cut(s) 755, 866, 983, 1300, 1432, 1469
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 3 cut(s) 1322, 1532, 1533
SduI GDGCHC 5 cut(s) 383, 496, 1210, 1539, 1562
SfaNI GCATC 3 cut(s) 348, 1024, 1406
SfcI CTRYAG 3 cut(s) 116, 142, 672
Sfr274I CTCGAG 2 cut(s) 254, 495
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 4 cut(s) 755, 983, 1300, 1469
SlaI CTCGAG 2 cut(s) 254, 495
SmaI CCCGGG 1 cut(s) 1533
SmlI CTYRAG 4 cut(s) 128, 254, 495, 790
SmoI CTYRAG 4 cut(s) 128, 254, 495, 790
SsiI CCGC 2 cut(s) 643, 767
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 621, 722, 740, 1113
SstI GAGCTC 2 cut(s) 1210, 1562
StyD4I CCNGG 3 cut(s) 1320, 1530, 1531
StyI CCWWGG 3 cut(s) 899, 1029, 1112
TaaI ACNGT 2 cut(s) 427, 676
TaiI ACGT 4 cut(s) 171, 239, 298, 640
TaqI TCGA 7 cut(s) 71, 255, 496, 542, 823, 862, 1463
TatI WGTACW 1 cut(s) 1507
TfiI GAWTC 3 cut(s) 98, 1158, 1455
Tru1I TTAA 3 cut(s) 162, 791, 1041
Tru9I TTAA 3 cut(s) 162, 791, 1041
TseFI GTSAC 2 cut(s) 781, 1253
TseI GCWGC 4 cut(s) 566, 851, 1388, 1418
Tsp45I GTSAC 2 cut(s) 781, 1253
TspDTI ATGAA 7 cut(s) 438, 726, 777, 799, 1249, 1356, 1468
TspMI CCCGGG 1 cut(s) 1531
Van91I CCANNNNNTGG 2 cut(s) 992, 1250
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 4 cut(s) 755, 983, 1300, 1469
VspI ATTAAT 1 cut(s) 1041
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 1 cut(s) 936
XbaI TCTAGA 1 cut(s) 721
XceI RCATGY 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XhoI CTCGAG 2 cut(s) 254, 495
XmaI CCCGGG 1 cut(s) 1531
XmaJI CCTAGG 1 cut(s) 1112
XmnI GAANNNNTTC 2 cut(s) 602, 717
XspI CTAG 4 cut(s) 621, 722, 740, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.