Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006269

Physical Location & Seq

Genomic Coordinates Forward (+)

1319422 .. 1322873

Gene Structure 3452 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031007

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGGCAACTGCAAGAGCCAATGTCTTATTGCCTTCGTTTTGTTTCATAGCGCTTCTCGCTTCTGTGATATTTACTGGTTTTTCAGTAGAAGATCGAGCAATATACTTGGTTTTGATGGAAGAAGACTCAGTTGCATTCAATGGAGGCTCTCCATCTTTACGCCTCAACCTAAACAGTGAAGTTTCGAAGGCTCATGTAAAGCAATTAGAGGATTCTCATGATCAACTTCTACAGAGCAATCTTGAGACCGGAAGCCACAGCAAGCTTTACAGCTTTAAACACGTTGCAAACGGCTTTGCTGTCCATACAACCCCTACTCAGGTTGAAAAGCTCAAATATGCTCCAGCAGTAAAGCTGGTTGAGAGAGATAGAGGAGCAAAGTTGATGACAACGTACACTCCTCGCTTCCTTGAGTTACCTGAAGGAGTATGGACACAAGAAGGAGGCCAACGTAATGCTGGTGAAGGAATTGTGATTGGTTTTGTGGATAGTGGCATCAATCCTGCACATCGAAGTTTTGCTTATAATCCTTTGAGCCCTTTCACCTCCAATATTTCTCATTTTTCGGGCGCTTGTGAGACTGGTCCTCTATTCCCAGCAACGTCATGCAATGGTAAAATTGTTTCAGCTAAGTTTTTCTCAGCTGGGGCTCGAGCCATTGCTACTCTCAACGCTTCGGTGGATTTTCTTTCACCCTTCGACGCAGATGGACATGGCAGCGCATGCTTTTTTCAGTCATGTGGCATCAGTTGCTGCTGGAAATGCTGGAAATGCTTCAGTTATGGACGAGCTGGTGGAATGGCACCACGTGCGCGGATTGCTGTATATAAAGCTGTGTATCCTACAGTAGGAACTTTGGCAGATGTAGTTTCTGCAATTGATGAAGCAATTCGAGATGGAGTGGATGTCCTAGCACTCTCTGTCGGACCAGATGAACCGCCATTGGGTAAAGTGACCTTCTTAAGCATGTTTGATGTGTTCGTGCTTAATCGAGGGCCAAACCCTTCAAGTGTAGTGTCTTACAGCCCTTGGGCAGTAGGTGTAGCTTCTTCCGGCACAGATAGAATTTATCCTTCTTCTATTGTTCTTGGAAATGGAGAAAGAATTGAAGGTGTGGGATTATCAGGACCAACTTTTGGATATAGGTTACTTCAACATAGGTTGGTTTTGGCCAAGGATGCAATTAATCCAATTGGGTCATTCCCAAGAACCTATGTTGAAGAGTGCCAATTTCCACAAGCATTGGATACTAGGTTAGTTATAGGTAGTATTGTCATCTGCACATTCTCGGAGGGATTCTATAACGGGAGATCCACCCTTAATGCCATCGTCAACACGGCAAGAGCTCTCGGATTTATGGGTTTTGTTCTTGTTGCAAATCCAGCCTATGGTGACTTCATTGCAGAGCCTGTTCCTTTTGCTGTTTCTGGCATTTTGGTCCCGAGTGTAACCAATTCCCAGGATGAGAGAGGATTTCCTACTAGACTTGGAGCAAGAGCATCAATCGGAGAAGGAAGAGCTGCTTCATTCATGGGCCAAGCACCTATTATTAGCAGATTCTCTTCGAGAGGGCCAGATTTCATTGACAGAGACAGGAAACCTGCTGATGTACTAAAGCCTGATATTCTTGCCCCAGGGCACCAAATCTGGGGAGCTTGGAGCCCCATTAGTGCCTTAGAACCCTCATTAGTGGGACACAGTTTTGCACTACTTTCTGGTACAAGCATGGCAACACCTCAAATCGCTGGAATAGCAGCACTTATTAAGCAGTACAATCCTTCATGGACTCCTTCCATGATTGCATCTGCGATTTCCACCACGGCAACCAATCAATATCCCTCCACTCCTTTTGATTTTGGAGCTGGCTTTGTCAGTCCAAGTCATGCCATGGATCCGGGTCTAGTCTTATCTTCAGGATATGAAAACTACGTCAGCTTCTTGTGCTCATTGCATGATACCAATCCAAGTACAATAAGAACTGTCACTGGAGAATTGTGCAACAAGCCACTTGACCATCCAGCCAATCTGAACCTTCCTTCAGTAACAATATCAGCATTGAATGGGTCTCAAATAGTACGAAGGGTTTTCATGAACGTAGAGAGCAAGCCAGAGACTTACTCTAGCTCGGTGCTGCCACCAAATGGGACAACAGTAGACATATCTCCACCTTCGTTTACCATAGCTCAACAAGGCACTAAAGAGCTAGTTATACAGTTCAATGTGACCCAAGCACTGGACCAATTCACCTTTGGTGAAATTGTTCTTACAGGAAGTTTAAATCATATTGTCAGGGTACCTTTATCAGTTTTTCCAGAGTCTATGACAAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0032501 GO:0032502 GO:0042335 GO:0043170 GO:0044238 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

778

Amino Acids

83.11

Weight (kDa)

6.22

Isoelectric Point (pI)

32.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	62 - 128	2.6e-11	Peptidase inhibitor I9
Peptidase_S8	PF00082	154 - 622	1.5e-27	Subtilase family
fn3_6	PF17766	675 - 769	5.7e-17	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006269)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62340
fragaria_vesca	FvH4_3g01420
malus_domestica	MD05G1352200.v1.1 MD10G1326200.v1.1
prunus_persica	Prupe.4G015500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002151 RchiOBHm_Chr5g0002191
rosa_laevigata	RLG00000031001 RLG00000031002 RLG00000031007
rosa_multiflora	Rmu_co8154878.1_g000001 Rmu_sc0001492.1_g000001 Rmu_sc0007900.1_g000001 Rmu_sc0013155.1_g000003 Rmu_ssc0000001.1_g000005
rosa_roxburghii	Rroxscaffold_1G00073760
rosa_rugosa	Rorug04G0394700
rosa_samantha	Rh5AG016500 Rh5AG016600 Rh5AG017100 Rh5BG020000 Rh5CG017800 Rh5CG017900 Rh5CG018800 Rh5DG018000
rosa_wichuraiana	Rw2G029690 Rw5G001610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	525
Acc36I	ACCTGC	1 cut(s)	1609
Acc65I	GGTACC	1 cut(s)	2293
AccB1I	GGYRCC	3 cut(s)	802, 1638, 2293
AccB7I	CCANNNNNTGG	4 cut(s)	1136, 1388, 2141, 2233
AccI	GTMKAC	1 cut(s)	2154
AccII	CGCG	1 cut(s)	814
AciI	CCGC	2 cut(s)	814, 938
AclWI	GGATC	3 cut(s)	1305, 1886, 1899
AcoI	YGGCCR	1 cut(s)	1170
AcsI	RAATTY	1 cut(s)	1065
AcuI	CTGAAG	4 cut(s)	441, 760, 1896, 2022
AcvI	CACGTG	1 cut(s)	809
AdeI	CACNNNGTG	1 cut(s)	809
AfaI	GTAC	7 cut(s)	395, 1611, 1720, 1772, 1969, 2076, 2295
AfeI	AGCGCT	1 cut(s)	51
AfiI	CCNNNNNNNGG	8 cut(s)	319, 848, 944, 1136, 1388, 1648, 2141, 2233
AflII	CTTAAG	1 cut(s)	961
AflIII	ACRYGT	1 cut(s)	280
AgsI	TTSAA	8 cut(s)	139, 326, 1008, 1109, 1154, 1220, 2059, 2218
AjnI	CCWGG	2 cut(s)	1458, 1633
Alw21I	GWGCWC	2 cut(s)	1348, 1946
Alw26I	GTCTC	5 cut(s)	239, 572, 1584, 2070, 2105
AlwI	GGATC	3 cut(s)	1305, 1886, 1899
AlwNI	CAGNNNCTG	2 cut(s)	753, 1409
Ama87I	CYCGRG	2 cut(s)	651, 1441
Aor51HI	AGCGCT	1 cut(s)	51
AoxI	GGCC	5 cut(s)	445, 995, 1170, 1534, 1571
ApeKI	GCWGC	5 cut(s)	717, 753, 1520, 1754, 2131
ApoI	RAATTY	1 cut(s)	1065
AseI	ATTAAT	1 cut(s)	1185
Asp700I	GAANNNNTTC	3 cut(s)	773, 888, 2259
Asp718I	GGTACC	1 cut(s)	2293
AspLEI	GCGC	4 cut(s)	52, 572, 722, 814
AspS9I	GGNCC	8 cut(s)	584, 926, 995, 1127, 1438, 1534, 1571, 2236
AsuC2I	CCSGG	1 cut(s)	1896
AsuHPI	GGTGA	6 cut(s)	473, 535, 684, 1403, 2236, 2264
AsuII	TTCGAA	1 cut(s)	185
AvaI	CYCGRG	2 cut(s)	651, 1441
AvaII	GGWCC	5 cut(s)	584, 926, 1127, 1438, 2236
BaeGI	GKGCMC	1 cut(s)	1641
BalI	TGGCCA	1 cut(s)	1172
BamHI	GGATCC	1 cut(s)	1891
BanI	GGYRCC	3 cut(s)	802, 1638, 2293
BanII	GRGCYC	4 cut(s)	539, 652, 1348, 1664
BbrPI	CACGTG	1 cut(s)	809
BbsI	GAAGAC	1 cut(s)	129
Bbv12I	GWGCWC	2 cut(s)	1348, 1946
BbvI	GCAGC	5 cut(s)	729, 740, 1507, 1766, 2118
BccI	CCATC	6 cut(s)	109, 160, 701, 890, 1334, 2022
BceAI	ACGGC	3 cut(s)	307, 1353, 1836
BciT130I	CCWGG	2 cut(s)	1460, 1635
BciVI	GTATCC	2 cut(s)	849, 1240
BclI	TGATCA	1 cut(s)	220
BcnI	CCSGG	1 cut(s)	1896
BcoDI	GTCTC	5 cut(s)	239, 572, 1584, 2070, 2105
BfaI	CTAG	6 cut(s)	911, 1251, 1482, 1901, 2121, 2204
BfmI	CTRYAG	2 cut(s)	230, 843
BfoI	RGCGCY	2 cut(s)	53, 573
BfrI	CTTAAG	1 cut(s)	961
BfuAI	ACCTGC	1 cut(s)	1609
BfuI	GTATCC	2 cut(s)	849, 1240
BisI	GCNGC	5 cut(s)	718, 754, 1521, 1755, 2132
BlsI	GCNGC	5 cut(s)	719, 755, 1522, 1756, 2133
Bme1390I	CCNGG	3 cut(s)	1460, 1635, 1896
Bme18I	GGWCC	5 cut(s)	584, 926, 1127, 1438, 2236
BmeT110I	CYCGRG	2 cut(s)	651, 1441
BmgT120I	GGNCC	8 cut(s)	584, 926, 995, 1127, 1438, 1534, 1571, 2236
BmiI	GGNNCC	6 cut(s)	804, 1440, 1640, 1661, 1893, 2295
BmrFI	CCNGG	3 cut(s)	1460, 1635, 1896
BmsI	GCATC	5 cut(s)	504, 753, 1168, 1508, 1811
BpiI	GAAGAC	1 cut(s)	129
BplI	GAGNNNNNCTC	2 cut(s)	2102, 2134
BpmI	CTGGAG	2 cut(s)	327, 2007
Bpu14I	TTCGAA	1 cut(s)	185
BpuEI	CTTGAG	2 cut(s)	263, 431
BpuMI	CCSGG	1 cut(s)	1896
BsaAI	YACGTR	1 cut(s)	809
BsaBI	GATNNNNATC	1 cut(s)	1959
BsaI	GGTCTC	2 cut(s)	239, 2070
BsaJI	CCNNGG	7 cut(s)	1028, 1173, 1458, 1633, 1634, 1818, 1887
BsaWI	WCCGGW	1 cut(s)	248
BsaXI	ACNNNNNCTCC	4 cut(s)	382, 412, 891, 921
Bsc4I	CCNNNNNNNGG	8 cut(s)	319, 848, 944, 1136, 1388, 1648, 2141, 2233
Bse1I	ACTGG	4 cut(s)	79, 586, 1990, 2238
Bse3DI	GCAATG	4 cut(s)	616, 657, 1398, 1946
Bse8I	GATNNNNATC	1 cut(s)	1959
BseBI	CCWGG	2 cut(s)	1460, 1635
BseDI	CCNNGG	7 cut(s)	1028, 1173, 1458, 1633, 1634, 1818, 1887
BseGI	GGATG	4 cut(s)	910, 1183, 1468, 2014
BseJI	GATNNNNATC	1 cut(s)	1959
BseLI	CCNNNNNNNGG	8 cut(s)	319, 848, 944, 1136, 1388, 1648, 2141, 2233
BseMI	GCAATG	4 cut(s)	616, 657, 1398, 1946
BseMII	CTCAG	3 cut(s)	141, 332, 654
BseNI	ACTGG	4 cut(s)	79, 586, 1990, 2238
BseRI	GAGGAG	2 cut(s)	387, 390
BseSI	GKGCMC	1 cut(s)	1641
BseXI	GCAGC	5 cut(s)	729, 740, 1507, 1766, 2118
BseYI	CCCAGC	2 cut(s)	595, 644
BsgI	GTGCAG	2 cut(s)	489, 1264
Bsh1236I	CGCG	1 cut(s)	814
BshFI	GGCC	5 cut(s)	447, 997, 1172, 1536, 1573
BshNI	GGYRCC	3 cut(s)	802, 1638, 2293
BsiHKAI	GWGCWC	2 cut(s)	1348, 1946
BsiHKCI	CYCGRG	2 cut(s)	651, 1441
BsiSI	CCGG	3 cut(s)	249, 1053, 1895
BslFI	GGGAC	3 cut(s)	1424, 1707, 2158
BslI	CCNNNNNNNGG	8 cut(s)	319, 848, 944, 1136, 1388, 1648, 2141, 2233
BsmAI	GTCTC	5 cut(s)	239, 572, 1584, 2070, 2105
BsmFI	GGGAC	3 cut(s)	1424, 1707, 2158
BsmI	GAATGC	1 cut(s)	134
BsnI	GGCC	5 cut(s)	447, 997, 1172, 1536, 1573
Bso31I	GGTCTC	2 cut(s)	239, 2070
BsoBI	CYCGRG	2 cut(s)	651, 1441
Bsp119I	TTCGAA	1 cut(s)	185
Bsp1286I	GDGCHC	6 cut(s)	539, 652, 1348, 1641, 1664, 1946
Bsp143I	GATC	4 cut(s)	91, 220, 1310, 1891
Bsp19I	CCATGG	1 cut(s)	1887
BspACI	CCGC	2 cut(s)	814, 938
BspANI	GGCC	5 cut(s)	447, 997, 1172, 1536, 1573
BspCNI	CTCAG	3 cut(s)	140, 331, 653
BspFNI	CGCG	1 cut(s)	814
BspHI	TCATGA	2 cut(s)	217, 2088
BspLI	GGNNCC	6 cut(s)	804, 1440, 1640, 1661, 1893, 2295
BspMI	ACCTGC	1 cut(s)	1609
BspPI	GGATC	3 cut(s)	1305, 1886, 1899
BspQI	GCTCTTC	1 cut(s)	1510
BspT104I	TTCGAA	1 cut(s)	185
BspT107I	GGYRCC	3 cut(s)	802, 1638, 2293
BspTI	CTTAAG	1 cut(s)	961
BspTNI	GGTCTC	2 cut(s)	239, 2070
BsrDI	GCAATG	4 cut(s)	616, 657, 1398, 1946
BsrI	ACTGG	4 cut(s)	79, 586, 1990, 2238
BssECI	CCNNGG	7 cut(s)	1028, 1173, 1458, 1633, 1634, 1818, 1887
BssMI	GATC	4 cut(s)	91, 220, 1310, 1891
BssT1I	CCWWGG	3 cut(s)	1028, 1173, 1887
Bst2UI	CCWGG	2 cut(s)	1460, 1635
Bst4CI	ACNGT	6 cut(s)	176, 847, 1700, 1981, 2152, 2214
Bst6I	CTCTTC	3 cut(s)	1215, 1510, 1567
BstAFI	CTTAAG	1 cut(s)	961
BstAPI	GCANNNNNTGC	3 cut(s)	723, 750, 809
BstBAI	YACGTR	1 cut(s)	809
BstBI	TTCGAA	1 cut(s)	185
BstC8I	GCNNGC	4 cut(s)	263, 724, 1864, 2105
BstDEI	CTNAG	5 cut(s)	127, 318, 630, 640, 1675
BstDSI	CCRYGG	2 cut(s)	1818, 1887
BstENI	CCTNNNNNAGG	1 cut(s)	846
BstF5I	GGATG	4 cut(s)	910, 1183, 1468, 2014
BstFNI	CGCG	1 cut(s)	814
BstH2I	RGCGCY	2 cut(s)	53, 573
BstHHI	GCGC	4 cut(s)	52, 572, 722, 814
BstKTI	GATC	4 cut(s)	94, 223, 1313, 1894
BstMAI	GTCTC	5 cut(s)	239, 572, 1584, 2070, 2105
BstMBI	GATC	4 cut(s)	91, 220, 1310, 1891
BstNI	CCWGG	2 cut(s)	1460, 1635
BstNSI	RCATGY	2 cut(s)	726, 970
BstSCI	CCNGG	3 cut(s)	1458, 1633, 1894
BstSFI	CTRYAG	2 cut(s)	230, 843
BstSLI	GKGCMC	1 cut(s)	1641
BstUI	CGCG	1 cut(s)	814
BstV1I	GCAGC	5 cut(s)	729, 740, 1507, 1766, 2118
BstV2I	GAAGAC	1 cut(s)	129
BstX2I	RGATCY	2 cut(s)	1310, 1891
BstYI	RGATCY	2 cut(s)	1310, 1891
BsuI	GTATCC	2 cut(s)	849, 1240
BsuRI	GGCC	5 cut(s)	447, 997, 1172, 1536, 1573
BtgI	CCRYGG	2 cut(s)	1818, 1887
BtsCI	GGATG	4 cut(s)	910, 1183, 1468, 2014
BtsIMutI	CAGTG	3 cut(s)	181, 1983, 2231
BveI	ACCTGC	1 cut(s)	1609
Cac8I	GCNNGC	4 cut(s)	263, 724, 1864, 2105
CaiI	CAGNNNCTG	2 cut(s)	753, 1409
CciI	TCATGA	2 cut(s)	217, 2088
CfoI	GCGC	4 cut(s)	52, 572, 722, 814
Cfr13I	GGNCC	8 cut(s)	584, 926, 995, 1127, 1438, 1534, 1571, 2236
CseI	GACGC	1 cut(s)	710
Csp6I	GTAC	7 cut(s)	394, 1610, 1719, 1771, 1968, 2075, 2294
CspCI	CAANNNNNGTGG	4 cut(s)	1224, 1259, 1831, 1866
CviQI	GTAC	7 cut(s)	394, 1610, 1719, 1771, 1968, 2075, 2294
DdeI	CTNAG	5 cut(s)	127, 318, 630, 640, 1675
DpnI	GATC	4 cut(s)	93, 222, 1312, 1893
DpnII	GATC	4 cut(s)	91, 220, 1310, 1891
DraI	TTTAAA	2 cut(s)	277, 2277
DraIII	CACNNNGTG	1 cut(s)	809
EaeI	YGGCCR	1 cut(s)	1170
Eam1104I	CTCTTC	3 cut(s)	1215, 1510, 1567
EarI	CTCTTC	3 cut(s)	1215, 1510, 1567
Ecl136II	GAGCTC	1 cut(s)	1346
Eco130I	CCWWGG	3 cut(s)	1028, 1173, 1887
Eco24I	GRGCYC	4 cut(s)	539, 652, 1348, 1664
Eco31I	GGTCTC	2 cut(s)	239, 2070
Eco47I	GGWCC	5 cut(s)	584, 926, 1127, 1438, 2236
Eco47III	AGCGCT	1 cut(s)	51
Eco53kI	GAGCTC	1 cut(s)	1346
Eco57I	CTGAAG	4 cut(s)	441, 760, 1896, 2022
Eco72I	CACGTG	1 cut(s)	809
Eco88I	CYCGRG	2 cut(s)	651, 1441
EcoICRI	GAGCTC	1 cut(s)	1346
EcoNI	CCTNNNNNAGG	1 cut(s)	846
EcoRII	CCWGG	2 cut(s)	1458, 1633
EcoT14I	CCWWGG	3 cut(s)	1028, 1173, 1887
EcoT38I	GRGCYC	4 cut(s)	539, 652, 1348, 1664
ErhI	CCWWGG	3 cut(s)	1028, 1173, 1887
FalI	AAGNNNNNCTT	4 cut(s)	505, 537, 1507, 1539
FaqI	GGGAC	3 cut(s)	1424, 1707, 2158
FbaI	TGATCA	1 cut(s)	220
FblI	GTMKAC	1 cut(s)	2154
Fnu4HI	GCNGC	5 cut(s)	718, 754, 1521, 1755, 2132
FokI	GGATG	4 cut(s)	917, 1190, 1475, 2001
FriOI	GRGCYC	4 cut(s)	539, 652, 1348, 1664
Fsp4HI	GCNGC	5 cut(s)	718, 754, 1521, 1755, 2132
FspBI	CTAG	6 cut(s)	911, 1251, 1482, 1901, 2121, 2204
GlaI	GCGC	4 cut(s)	51, 571, 721, 813
GluI	GCNGC	5 cut(s)	718, 754, 1521, 1755, 2132
GsaI	CCCAGC	2 cut(s)	599, 648
GsuI	CTGGAG	2 cut(s)	327, 2007
HaeII	RGCGCY	2 cut(s)	53, 573
HaeIII	GGCC	5 cut(s)	447, 997, 1172, 1536, 1573
HapII	CCGG	3 cut(s)	249, 1053, 1895
HgaI	GACGC	1 cut(s)	710
HhaI	GCGC	4 cut(s)	52, 572, 722, 814
Hin6I	GCGC	4 cut(s)	50, 570, 720, 812
HinP1I	GCGC	4 cut(s)	50, 570, 720, 812
HincII	GTYRAC	1 cut(s)	1333
HindII	GTYRAC	1 cut(s)	1333
HindIII	AAGCTT	1 cut(s)	263
HinfI	GANTC	6 cut(s)	125, 212, 1296, 1557, 1786, 2315
HpaII	CCGG	3 cut(s)	249, 1053, 1895
HphI	GGTGA	6 cut(s)	473, 535, 684, 1403, 2236, 2264
Hpy166II	GTNNAC	4 cut(s)	396, 1333, 2155, 2175
Hpy188I	TCNGA	5 cut(s)	926, 1291, 1352, 1508, 2028
Hpy188III	TCNNGA	9 cut(s)	218, 242, 893, 1125, 1441, 1566, 1914, 2089, 2312
Hpy8I	GTNNAC	4 cut(s)	396, 1333, 2155, 2175
Hpy99I	CGWCG	1 cut(s)	704
HpyCH4III	ACNGT	6 cut(s)	176, 847, 1700, 1981, 2152, 2214
HpyCH4IV	ACGT	7 cut(s)	282, 392, 451, 602, 808, 1929, 2094
HpyF3I	CTNAG	5 cut(s)	127, 318, 630, 640, 1675
HpySE526I	ACGT	7 cut(s)	282, 392, 451, 602, 808, 1929, 2094
HspAI	GCGC	4 cut(s)	50, 570, 720, 812
KpnI	GGTACC	1 cut(s)	2297
Ksp22I	TGATCA	1 cut(s)	220
Kzo9I	GATC	4 cut(s)	91, 220, 1310, 1891
LguI	GCTCTTC	1 cut(s)	1510
LmnI	GCTCC	6 cut(s)	346, 374, 1490, 1652, 1659, 1859
Lsp1109I	GCAGC	5 cut(s)	729, 740, 1507, 1766, 2118
LweI	GCATC	5 cut(s)	504, 753, 1168, 1508, 1811
MaeI	CTAG	6 cut(s)	911, 1251, 1482, 1901, 2121, 2204
MaeII	ACGT	7 cut(s)	282, 392, 451, 602, 808, 1929, 2094
MaeIII	GTNAC	8 cut(s)	414, 952, 1146, 1391, 1447, 1981, 2041, 2221
MalI	GATC	4 cut(s)	93, 222, 1312, 1893
MboI	GATC	4 cut(s)	91, 220, 1310, 1891
MboII	GAAGA	9 cut(s)	101, 131, 134, 1041, 1068, 1232, 1527, 1554, 1902
MfeI	CAATTG	2 cut(s)	876, 1191
MflI	RGATCY	2 cut(s)	1310, 1891
MhlI	GDGCHC	6 cut(s)	539, 652, 1348, 1641, 1664, 1946
MlsI	TGGCCA	1 cut(s)	1172
MluNI	TGGCCA	1 cut(s)	1172
MlyI	GAGTC	3 cut(s)	119, 1780, 2324
MmeI	TCCRAC	1 cut(s)	904
Mox20I	TGGCCA	1 cut(s)	1172
MroXI	GAANNNNTTC	3 cut(s)	773, 888, 2259
MscI	TGGCCA	1 cut(s)	1172
MseI	TTAA	7 cut(s)	276, 962, 987, 1185, 1320, 1764, 2276
Msp20I	TGGCCA	1 cut(s)	1172
MspA1I	CMGCKG	1 cut(s)	644
MspCI	CTTAAG	1 cut(s)	961
MspI	CCGG	3 cut(s)	249, 1053, 1895
MspR9I	CCNGG	3 cut(s)	1460, 1635, 1896
MunI	CAATTG	2 cut(s)	876, 1191
Mva1269I	GAATGC	1 cut(s)	134
MvaI	CCWGG	2 cut(s)	1460, 1635
MvnI	CGCG	1 cut(s)	814
NciI	CCSGG	1 cut(s)	1896
NcoI	CCATGG	1 cut(s)	1887
NdeII	GATC	4 cut(s)	91, 220, 1310, 1891
NlaIV	GGNNCC	6 cut(s)	804, 1440, 1640, 1661, 1893, 2295
NmuCI	GTSAC	4 cut(s)	952, 1391, 1981, 2221
NspI	RCATGY	2 cut(s)	726, 970
NspV	TTCGAA	1 cut(s)	185
PaeI	GCATGC	1 cut(s)	726
PaeR7I	CTCGAG	1 cut(s)	651
PagI	TCATGA	2 cut(s)	217, 2088
PasI	CCCWGGG	1 cut(s)	1634
PciSI	GCTCTTC	1 cut(s)	1510
PctI	GAATGC	1 cut(s)	134
PdmI	GAANNNNTTC	3 cut(s)	773, 888, 2259
PfeI	GAWTC	3 cut(s)	212, 1296, 1557
PflMI	CCANNNNNTGG	4 cut(s)	1136, 1388, 2141, 2233
PkrI	GCNGC	5 cut(s)	719, 755, 1522, 1756, 2133
PleI	GAGTC	3 cut(s)	119, 1780, 2323
PmaCI	CACGTG	1 cut(s)	809
PmlI	CACGTG	1 cut(s)	809
PpsI	GAGTC	3 cut(s)	119, 1780, 2323
Ppu21I	YACGTR	1 cut(s)	809
PshBI	ATTAAT	1 cut(s)	1185
PsiI	TTATAA	1 cut(s)	525
Psp124BI	GAGCTC	1 cut(s)	1348
Psp6I	CCWGG	2 cut(s)	1458, 1633
PspCI	CACGTG	1 cut(s)	809
PspFI	CCCAGC	2 cut(s)	595, 644
PspGI	CCWGG	2 cut(s)	1458, 1633
PspN4I	GGNNCC	6 cut(s)	804, 1440, 1640, 1661, 1893, 2295
PspPI	GGNCC	8 cut(s)	584, 926, 995, 1127, 1438, 1534, 1571, 2236
PspXI	VCTCGAGB	1 cut(s)	651
PstNI	CAGNNNCTG	2 cut(s)	753, 1409
PsuI	RGATCY	2 cut(s)	1310, 1891
PvuII	CAGCTG	1 cut(s)	644
RsaI	GTAC	7 cut(s)	395, 1611, 1720, 1772, 1969, 2076, 2295
RsaNI	GTAC	7 cut(s)	394, 1610, 1719, 1771, 1968, 2075, 2294
SacI	GAGCTC	1 cut(s)	1348
SapI	GCTCTTC	1 cut(s)	1510
SaqAI	TTAA	7 cut(s)	276, 962, 987, 1185, 1320, 1764, 2276
SatI	GCNGC	5 cut(s)	718, 754, 1521, 1755, 2132
Sau3AI	GATC	4 cut(s)	91, 220, 1310, 1891
Sau96I	GGNCC	8 cut(s)	584, 926, 995, 1127, 1438, 1534, 1571, 2236
SchI	GAGTC	3 cut(s)	119, 1780, 2324
ScrFI	CCNGG	3 cut(s)	1460, 1635, 1896
SduI	GDGCHC	6 cut(s)	539, 652, 1348, 1641, 1664, 1946
SfaNI	GCATC	5 cut(s)	504, 753, 1168, 1508, 1811
SfcI	CTRYAG	2 cut(s)	230, 843
Sfr274I	CTCGAG	1 cut(s)	651
SfuI	TTCGAA	1 cut(s)	185
SinI	GGWCC	5 cut(s)	584, 926, 1127, 1438, 2236
SlaI	CTCGAG	1 cut(s)	651
SmlI	CTYRAG	4 cut(s)	242, 410, 651, 961
SmoI	CTYRAG	4 cut(s)	242, 410, 651, 961
SphI	GCATGC	1 cut(s)	726
SsiI	CCGC	2 cut(s)	814, 938
SspI	AATATT	1 cut(s)	553
SspMI	CTAG	6 cut(s)	911, 1251, 1482, 1901, 2121, 2204
SstI	GAGCTC	1 cut(s)	1348
StyD4I	CCNGG	3 cut(s)	1458, 1633, 1894
StyI	CCWWGG	3 cut(s)	1028, 1173, 1887
TaaI	ACNGT	6 cut(s)	176, 847, 1700, 1981, 2152, 2214
TaiI	ACGT	7 cut(s)	285, 395, 454, 605, 811, 1932, 2097
TaqI	TCGA	8 cut(s)	94, 185, 511, 652, 699, 892, 991, 1565
TatI	WGTACW	3 cut(s)	1609, 1770, 1967
TfiI	GAWTC	3 cut(s)	212, 1296, 1557
Tru1I	TTAA	7 cut(s)	276, 962, 987, 1185, 1320, 1764, 2276
Tru9I	TTAA	7 cut(s)	276, 962, 987, 1185, 1320, 1764, 2276
TscAI	CASTG	3 cut(s)	181, 1990, 2238
TseFI	GTSAC	4 cut(s)	952, 1391, 1981, 2221
TseI	GCWGC	5 cut(s)	717, 753, 1520, 1754, 2131
Tsp45I	GTSAC	4 cut(s)	952, 1391, 1981, 2221
TspRI	CASTG	3 cut(s)	181, 1990, 2238
Van91I	CCANNNNNTGG	4 cut(s)	1136, 1388, 2141, 2233
Vha464I	CTTAAG	1 cut(s)	961
VpaK11BI	GGWCC	5 cut(s)	584, 926, 1127, 1438, 2236
VspI	ATTAAT	1 cut(s)	1185
XagI	CCTNNNNNAGG	1 cut(s)	846
XapI	RAATTY	1 cut(s)	1065
XceI	RCATGY	2 cut(s)	726, 970
XcmI	CCANNNNNNNNNTGG	2 cut(s)	455, 2246
XhoI	CTCGAG	1 cut(s)	651
XmiI	GTMKAC	1 cut(s)	2154
XmnI	GAANNNNTTC	3 cut(s)	773, 888, 2259
XspI	CTAG	6 cut(s)	911, 1251, 1482, 1901, 2121, 2204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.