Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006269

Physical Location & Seq

Genomic Coordinates Reverse (-)

40645042 .. 40648719

Gene Structure 3678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1326200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2541 bp

ATGGGAGGAAGGGCCAGTGTAATGCCTCCAATATTTTTCCTAGCGCTCATTGTTTCTGTCGGGTTCGTTAGCTTTGCGGAAGAGCGAGACTTGTACATGGTTTTGATGGAAGGAGACCCGGTCGCATTCCATGGAGGTTCTCGAATATCATCTCGTGAAGACGGAAGGCGTCTTGATCCAAACAGTGAAGTTTTCAAGGCCCATGCAAAGCGTTTGGTGGATTCTCATGACCAACTTCTACAGAGTGCTTTAGAGACGGGAAGCTACAGCAAGCTTTACAGCTTTAAACATATTGTAAACGGCTTTGCTGTCCATACCACCCCTTCCCAGGCTGAGAAGCTCAAAGCTGCTGCAGGAGTGAAGTTAGTTGAGAGAGACAGAGGAGCAAAGTTGATGACAACGTACACTCCTGAATTCCTTGGACTACCCCAAGGAGCATGGACACACGATGGAGGAGATGATCGTAGCGCCGGCGAGGGAATCGTGATTGGTTTTGTGGATTCAGGCATCAACCCCACACACCCCAGTTTTGCTAATGATCCCCTGCACCCTTTCACCGGAAATATCTCTCATTTTTTGGGATCTTGCGAAACGGGTCCTCGATTCCCAGCAAGTTCATGCAATGGGAAGATAGTCTCGGCAAAGTTCTTCTCAGCTGGGGCTGAAGCTGCTGCCACTCTTAACTCTACAGTGGATTTTCTTTCACCCTTTGATGCAGTTGGTCATGGAAGTCACGTGGCATCAACCGCTGCGGGTAATGCTGGAGTTCCAGTGGTTGTGAATGGTTACTACTACGGAGAAGCAAGTGGAATGGCTCCACGGGCACGAATTGCGGTGTATAAGGCTGTGTATCCTACAGTGGGAACTTTAACTGATGTGGTTTCAGCAATCGATCAAGCAATTCTGGATGGAGTGGACATCCTAACACTCTCCGTAGGGCCAGATGAACCGCCCGAAGACACGGTTACCTTCTTAAGCGCGTACGATATTGCCATGTTATCTGCTCGAAGAGCAGGGGTTTTCGTGGTGCAAGCAACAGGTAATCAAGGGCCAGGTATTTCAAGCGTAGTGTCTTACAGCCCTTGGGCTGTAGGTGTCGCTTCATCTGGCACAGATAGAACTTATCCGGGTTCTATTCTTCTCGGAAATGGCACAAAAATCGAAGGTGTGGGATTATCAGGACCGACTTTTGGATATGGTTTACTTCTGCATAAGTTGGTTTTGGCAAAGGATGCAGTGAAGGCAGATGGGACTTTCCCAAGGACTCCGCCTTATGTCGAAGAGTGCCAATATCCAGAAGCATTGGATCACAATGTAGTACTAGGTAGCATCGTCATCTGCACCTTCTCGGAGGGATTCTACAACGGAACATCCACCCTCACTGCCATCATCAACACGGCAAAAGGTCTAGGGTTTATGGGTTTTATCTTTGTTGCAAATCCGGCCTACGGTGATTTCATTGCAGAACCTATTCCTTTTGTTGTTCCTGGCATTCTGGTCCCTAATTTAACCAATTCCCAGGAGAAGGGATTTGCAACTCGATTCGCGGCAGAAGCAGCTATCGGAGAAGGAAGAGTTGCTTCTTTCATGGGACAAGCGCCCGTTGTAAGCAGATTCTCTTCGAGGGGACCAGATTTTGTAAACACCTCCAGGACTCCTTGTGATGTATTAAAGCCGGATATTCTTGCCCCAGGGCACCAAATTTGGGCAGCTTGGAGTCCCGTTAGCGCCTCAGAACCCGCTTTAACAGGTGATAAGAAGATGCAAGCCTGCAGCAATCTTTTGTGTTATTTCGGCAATACCTTGATATACAAGCAAATAATTAACAAACTTTGGTGTTTTTGTTGTTTAGGGTACAATTTTGCATTACAATCTGGTACAAGCATGGCAACACCTCATGTGGCGGGAATAGCAGCACTCGTCAAGCAGTACAATCCTTCATGGACTCCATCCATGATTGCGTCCGCGATTTCCACCACTGCCACAAAGTACGACAAAAATGGGGAACTTATCATGGCGGAAGGATCTGGCATAGGCAGTACATATCCTTCCAGCCATTTTGATTTCGGAGCTGGACTAGTCAGTCCAAGTCGAGCCCTGGATCCAGGCCTAGTCTTACCTACCGGATATGAAGATTATATCAGCTTCTTGTGCTCATTGCCCGAAATCAGTCCAGCTGCAGTTAGAAATGCAACTGGTGAACCGTGCAATAACACGCTTGACCATCCGGCCAATTTGAACCTCCCTTCGATAACATTATCGGCATTGAGGGGGTCTCAAATCGTCCGACGGATTTTCAATAACGTGGGGAGCAAGCCAGAGACTTACGTTTGCTCTGCGGTGTCACCTAATGGGACAATAGTGGATCTGTGTCCGACTTGGTTTAGGATTGCTCCAGAAGGCATCCAAGAACTGCTTATACAAGTTAAGGTGACGCAAGCGATGGATGATTTTACATTTGGTGAAATTGTTCTTACTGGAAGTTTAAATCATATTGTCAGGATACCCATATCGGTTTTTCCTGTTTCGATATCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0032501 GO:0032502 GO:0042335 GO:0043170 GO:0044238 GO:0048856 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 Peptidase_S8

Protein Analysis

847

Amino Acids

89.51

Weight (kDa)

5.66

Isoelectric Point (pI)

32.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	65 - 131	1.6e-11	Peptidase inhibitor I9
Peptidase_S8	PF00082	158 - 668	1.5e-38	Subtilase family
fn3_6	PF17766	745 - 839	2.7e-14	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006269)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62340
fragaria_vesca	FvH4_3g01420
malus_domestica	MD05G1352200.v1.1 MD10G1326200.v1.1
prunus_persica	Prupe.4G015500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0002151 RchiOBHm_Chr5g0002191
rosa_laevigata	RLG00000031001 RLG00000031002 RLG00000031007
rosa_multiflora	Rmu_co8154878.1_g000001 Rmu_sc0001492.1_g000001 Rmu_sc0007900.1_g000001 Rmu_sc0013155.1_g000003 Rmu_ssc0000001.1_g000005
rosa_roxburghii	Rroxscaffold_1G00073760
rosa_rugosa	Rorug04G0394700
rosa_samantha	Rh5AG016500 Rh5AG016600 Rh5AG017100 Rh5BG020000 Rh5CG017800 Rh5CG017900 Rh5CG018800 Rh5DG018000
rosa_wichuraiana	Rw2G029690 Rw5G001610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2082
AccB1I	GGYRCC	1 cut(s)	1695
AccII	CGCG	3 cut(s)	980, 1547, 1967
AclWI	GGATC	8 cut(s)	170, 533, 589, 1314, 2032, 2096, 2109, 2373
AcoI	YGGCCR	1 cut(s)	2229
AcsI	RAATTY	2 cut(s)	413, 1701
AcuI	CTGAAG	1 cut(s)	684
AcvI	CACGTG	1 cut(s)	736
AcyI	GRCGYC	1 cut(s)	169
AfaI	GTAC	9 cut(s)	95, 404, 983, 1320, 1856, 1879, 1931, 1991, 2041
AfeI	AGCGCT	1 cut(s)	45
AfiI	CCNNNNNNNGG	7 cut(s)	328, 557, 860, 1190, 1448, 1525, 1705
AflII	CTTAAG	1 cut(s)	973
AgsI	TTSAA	4 cut(s)	196, 1062, 2239, 2299
AhlI	ACTAGT	1 cut(s)	2077
AjnI	CCWGG	8 cut(s)	327, 1051, 1486, 1518, 1649, 1690, 2097, 2104
Alw21I	GWGCWC	1 cut(s)	2156
Alw26I	GTCTC	7 cut(s)	81, 108, 248, 369, 640, 2280, 2315
AlwI	GGATC	8 cut(s)	170, 533, 589, 1314, 2032, 2096, 2109, 2373
Aor51HI	AGCGCT	1 cut(s)	45
AoxI	GGCC	7 cut(s)	12, 198, 938, 1049, 1443, 2107, 2229
ApoI	RAATTY	2 cut(s)	413, 1701
Asp700I	GAANNNNTTC	2 cut(s)	1470, 2469
AspLEI	GCGC	5 cut(s)	46, 470, 980, 1600, 1730
AspS9I	GGNCC	8 cut(s)	12, 199, 596, 938, 1049, 1181, 1498, 1628
AsuC2I	CCSGG	2 cut(s)	119, 1128
AsuHPI	GGTGA	8 cut(s)	547, 696, 1463, 1763, 2210, 2337, 2443, 2474
AvaII	GGWCC	4 cut(s)	596, 1181, 1498, 1628
BaeGI	GKGCMC	2 cut(s)	826, 1698
BamHI	GGATCC	1 cut(s)	2101
BanI	GGYRCC	1 cut(s)	1695
BanII	GRGCYC	1 cut(s)	2098
BarI	GAAGNNNNNNTAC	2 cut(s)	2032, 2064
BauI	CACGAG	1 cut(s)	153
BbrPI	CACGTG	1 cut(s)	736
BbsI	GAAGAC	2 cut(s)	165, 963
Bbv12I	GWGCWC	1 cut(s)	2156
BccI	CCATC	8 cut(s)	100, 443, 902, 1241, 1394, 1957, 2232, 2437
BceAI	ACGGC	2 cut(s)	316, 1413
BcgI	CGANNNNNNTGC	2 cut(s)	1141, 1175
BciT130I	CCWGG	8 cut(s)	329, 1053, 1488, 1520, 1651, 1692, 2099, 2106
BciVI	GTATCC	2 cut(s)	861, 2496
BcnI	CCSGG	2 cut(s)	119, 1128
BcoDI	GTCTC	7 cut(s)	81, 108, 248, 369, 640, 2280, 2315
BcuI	ACTAGT	1 cut(s)	2077
BfaI	CTAG	5 cut(s)	41, 1322, 1409, 2078, 2111
BfmI	CTRYAG	8 cut(s)	239, 265, 351, 687, 855, 1089, 1771, 2178
BfoI	RGCGCY	4 cut(s)	47, 471, 1601, 1731
BfrI	CTTAAG	1 cut(s)	973
BfuI	GTATCC	2 cut(s)	861, 2496
BmcAI	AGTACT	1 cut(s)	1320
Bme18I	GGWCC	4 cut(s)	596, 1181, 1498, 1628
BmgT120I	GGNCC	8 cut(s)	12, 199, 596, 938, 1049, 1181, 1498, 1628
BmiI	GGNNCC	6 cut(s)	597, 816, 1500, 1629, 1697, 2103
BmrI	ACTGGG	1 cut(s)	519
BmsI	GCATC	7 cut(s)	516, 703, 749, 1222, 1338, 1752, 2412
BmuI	ACTGGG	1 cut(s)	519
BpiI	GAAGAC	2 cut(s)	165, 963
BplI	GAGNNNNNCTC	2 cut(s)	2260, 2292
BpmI	CTGGAG	3 cut(s)	783, 1633, 2379
BpuMI	CCSGG	2 cut(s)	119, 1128
Bsa29I	ATCGAT	1 cut(s)	891
BsaAI	YACGTR	1 cut(s)	736
BsaHI	GRCGYC	1 cut(s)	169
BsaI	GGTCTC	2 cut(s)	108, 2280
BsaWI	WCCGGW	2 cut(s)	557, 2123
BsaXI	ACNNNNNCTCC	6 cut(s)	105, 135, 391, 421, 756, 786
Bsc4I	CCNNNNNNNGG	7 cut(s)	328, 557, 860, 1190, 1448, 1525, 1705
Bse118I	RCCGGY	1 cut(s)	470
Bse1I	ACTGG	5 cut(s)	15, 525, 770, 2200, 2482
Bse3DI	GCAATG	3 cut(s)	628, 1458, 2156
BseBI	CCWGG	8 cut(s)	329, 1053, 1488, 1520, 1651, 1692, 2099, 2106
BseCI	ATCGAT	1 cut(s)	891
BseGI	GGATG	8 cut(s)	913, 918, 1237, 1370, 1949, 2224, 2403, 2452
BseLI	CCNNNNNNNGG	7 cut(s)	328, 557, 860, 1190, 1448, 1525, 1705
BseMI	GCAATG	3 cut(s)	628, 1458, 2156
BseMII	CTCAG	3 cut(s)	324, 666, 1746
BseNI	ACTGG	5 cut(s)	15, 525, 770, 2200, 2482
BseRI	GAGGAG	2 cut(s)	396, 468
BseSI	GKGCMC	2 cut(s)	826, 1698
BseYI	CCCAGC	2 cut(s)	607, 656
BsgI	GTGCAG	2 cut(s)	530, 1324
Bsh1236I	CGCG	3 cut(s)	980, 1547, 1967
Bsh1285I	CGRYCG	1 cut(s)	123
BshFI	GGCC	7 cut(s)	14, 200, 940, 1051, 1445, 2109, 2231
BshNI	GGYRCC	1 cut(s)	1695
BshVI	ATCGAT	1 cut(s)	891
BsiEI	CGRYCG	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	2156
BsiSI	CCGG	8 cut(s)	119, 471, 558, 1127, 1442, 1676, 2124, 2228
BsiWI	CGTACG	1 cut(s)	981
BslFI	GGGAC	6 cut(s)	1264, 1484, 1605, 1641, 1704, 2368
BslI	CCNNNNNNNGG	7 cut(s)	328, 557, 860, 1190, 1448, 1525, 1705
BsmAI	GTCTC	7 cut(s)	81, 108, 248, 369, 640, 2280, 2315
BsmBI	CGTCTC	1 cut(s)	248
BsmFI	GGGAC	6 cut(s)	1264, 1484, 1605, 1641, 1704, 2368
BsmI	GAATGC	2 cut(s)	125, 1491
BsnI	GGCC	7 cut(s)	14, 200, 940, 1051, 1445, 2109, 2231
Bso31I	GGTCTC	2 cut(s)	108, 2280
Bsp1286I	GDGCHC	4 cut(s)	826, 1698, 2098, 2156
Bsp1407I	TGTACA	1 cut(s)	93
Bsp143I	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
Bsp19I	CCATGG	1 cut(s)	130
BspANI	GGCC	7 cut(s)	14, 200, 940, 1051, 1445, 2109, 2231
BspCNI	CTCAG	3 cut(s)	325, 665, 1745
BspDI	ATCGAT	1 cut(s)	891
BspFNI	CGCG	3 cut(s)	980, 1547, 1967
BspHI	TCATGA	1 cut(s)	226
BspLI	GGNNCC	6 cut(s)	597, 816, 1500, 1629, 1697, 2103
BspMAI	CTGCAG	3 cut(s)	355, 1775, 2182
BspPI	GGATC	8 cut(s)	170, 533, 589, 1314, 2032, 2096, 2109, 2373
BspQI	GCTCTTC	2 cut(s)	75, 1003
BspT107I	GGYRCC	1 cut(s)	1695
BspTI	CTTAAG	1 cut(s)	973
BspTNI	GGTCTC	2 cut(s)	108, 2280
BsrDI	GCAATG	3 cut(s)	628, 1458, 2156
BsrFI	RCCGGY	1 cut(s)	470
BsrGI	TGTACA	1 cut(s)	93
BsrI	ACTGG	5 cut(s)	15, 525, 770, 2200, 2482
BssAI	RCCGGY	1 cut(s)	470
BssMI	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
BssNI	GRCGYC	1 cut(s)	169
BssSI	CACGAG	1 cut(s)	153
BssT1I	CCWWGG	5 cut(s)	130, 418, 430, 1082, 1259
Bst2BI	CACGAG	1 cut(s)	153
Bst2UI	CCWGG	8 cut(s)	329, 1053, 1488, 1520, 1651, 1692, 2099, 2106
Bst4CI	ACNGT	6 cut(s)	185, 691, 859, 964, 1451, 2205
Bst6I	CTCTTC	5 cut(s)	75, 1003, 1275, 1567, 1624
BstACI	GRCGYC	1 cut(s)	169
BstAFI	CTTAAG	1 cut(s)	973
BstAPI	GCANNNNNTGC	2 cut(s)	830, 1232
BstAUI	TGTACA	1 cut(s)	93
BstBAI	YACGTR	1 cut(s)	736
BstC8I	GCNNGC	7 cut(s)	272, 472, 1032, 1767, 1771, 2315, 2439
BstDEI	CTNAG	3 cut(s)	333, 652, 1732
BstDSI	CCRYGG	2 cut(s)	130, 818
BstEII	GGTNACC	1 cut(s)	964
BstF5I	GGATG	8 cut(s)	913, 918, 1237, 1370, 1949, 2224, 2403, 2452
BstFNI	CGCG	3 cut(s)	980, 1547, 1967
BstH2I	RGCGCY	4 cut(s)	47, 471, 1601, 1731
BstHHI	GCGC	5 cut(s)	46, 470, 980, 1600, 1730
BstKTI	GATC	9 cut(s)	178, 463, 541, 584, 895, 1309, 2027, 2104, 2368
BstMAI	GTCTC	7 cut(s)	81, 108, 248, 369, 640, 2280, 2315
BstMBI	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
BstMCI	CGRYCG	1 cut(s)	123
BstNI	CCWGG	8 cut(s)	329, 1053, 1488, 1520, 1651, 1692, 2099, 2106
BstPI	GGTNACC	1 cut(s)	964
BstSFI	CTRYAG	8 cut(s)	239, 265, 351, 687, 855, 1089, 1771, 2178
BstSLI	GKGCMC	2 cut(s)	826, 1698
BstUI	CGCG	3 cut(s)	980, 1547, 1967
BstV2I	GAAGAC	2 cut(s)	165, 963
BstX2I	RGATCY	4 cut(s)	581, 2024, 2101, 2365
BstYI	RGATCY	4 cut(s)	581, 2024, 2101, 2365
Bsu15I	ATCGAT	1 cut(s)	891
BsuI	GTATCC	2 cut(s)	861, 2496
BsuRI	GGCC	7 cut(s)	14, 200, 940, 1051, 1445, 2109, 2231
BsuTUI	ATCGAT	1 cut(s)	891
BtgI	CCRYGG	2 cut(s)	130, 818
BtgZI	GCGATG	1 cut(s)	2456
BtsCI	GGATG	8 cut(s)	913, 918, 1237, 1370, 1949, 2224, 2403, 2452
BtsI	GCAGTG	3 cut(s)	1242, 1380, 1977
BtsIMutI	CAGTG	8 cut(s)	22, 190, 696, 777, 864, 1242, 1380, 1977
Cac8I	GCNNGC	7 cut(s)	272, 472, 1032, 1767, 1771, 2315, 2439
CciI	TCATGA	1 cut(s)	226
CfoI	GCGC	5 cut(s)	46, 470, 980, 1600, 1730
Cfr10I	RCCGGY	1 cut(s)	470
Cfr13I	GGNCC	8 cut(s)	12, 199, 596, 938, 1049, 1181, 1498, 1628
ClaI	ATCGAT	1 cut(s)	891
CseI	GACGC	3 cut(s)	158, 1950, 2443
Csp6I	GTAC	9 cut(s)	94, 403, 982, 1319, 1855, 1878, 1930, 1990, 2040
CviQI	GTAC	9 cut(s)	94, 403, 982, 1319, 1855, 1878, 1930, 1990, 2040
DdeI	CTNAG	3 cut(s)	333, 652, 1732
DpnI	GATC	9 cut(s)	177, 462, 540, 583, 894, 1308, 2026, 2103, 2367
DpnII	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
DraI	TTTAAA	2 cut(s)	286, 2487
DrdI	GACNNNNNNGTC	1 cut(s)	2082
DseDI	GACNNNNNNGTC	1 cut(s)	2082
EaeI	YGGCCR	1 cut(s)	2229
Eam1104I	CTCTTC	5 cut(s)	75, 1003, 1275, 1567, 1624
EarI	CTCTTC	5 cut(s)	75, 1003, 1275, 1567, 1624
EciI	GGCGGA	2 cut(s)	1257, 2033
Eco130I	CCWWGG	5 cut(s)	130, 418, 430, 1082, 1259
Eco147I	AGGCCT	1 cut(s)	2109
Eco24I	GRGCYC	1 cut(s)	2098
Eco31I	GGTCTC	2 cut(s)	108, 2280
Eco32I	GATATC	1 cut(s)	2532
Eco47I	GGWCC	4 cut(s)	596, 1181, 1498, 1628
Eco47III	AGCGCT	1 cut(s)	45
Eco57I	CTGAAG	1 cut(s)	684
Eco72I	CACGTG	1 cut(s)	736
Eco91I	GGTNACC	1 cut(s)	964
EcoO109I	RGGNCCY	1 cut(s)	596
EcoO65I	GGTNACC	1 cut(s)	964
EcoRI	GAATTC	1 cut(s)	413
EcoRII	CCWGG	8 cut(s)	327, 1051, 1486, 1518, 1649, 1690, 2097, 2104
EcoRV	GATATC	1 cut(s)	2532
EcoT14I	CCWWGG	5 cut(s)	130, 418, 430, 1082, 1259
EcoT38I	GRGCYC	1 cut(s)	2098
ErhI	CCWWGG	5 cut(s)	130, 418, 430, 1082, 1259
Esp3I	CGTCTC	1 cut(s)	248
FalI	AAGNNNNNCTT	4 cut(s)	1564, 1596, 2400, 2432
FaqI	GGGAC	6 cut(s)	1264, 1484, 1605, 1641, 1704, 2368
FauI	CCCGC	3 cut(s)	745, 1747, 1897
FokI	GGATG	8 cut(s)	905, 920, 1244, 1357, 1936, 2211, 2390, 2459
FriOI	GRGCYC	1 cut(s)	2098
FspBI	CTAG	5 cut(s)	41, 1322, 1409, 2078, 2111
GlaI	GCGC	5 cut(s)	45, 469, 979, 1599, 1729
GsaI	CCCAGC	2 cut(s)	611, 660
GsuI	CTGGAG	3 cut(s)	783, 1633, 2379
HaeII	RGCGCY	4 cut(s)	47, 471, 1601, 1731
HaeIII	GGCC	7 cut(s)	14, 200, 940, 1051, 1445, 2109, 2231
HapII	CCGG	8 cut(s)	119, 471, 558, 1127, 1442, 1676, 2124, 2228
HgaI	GACGC	3 cut(s)	158, 1950, 2443
HhaI	GCGC	5 cut(s)	46, 470, 980, 1600, 1730
Hin1I	GRCGYC	1 cut(s)	169
Hin6I	GCGC	5 cut(s)	44, 468, 978, 1598, 1728
HinP1I	GCGC	5 cut(s)	44, 468, 978, 1598, 1728
HindIII	AAGCTT	1 cut(s)	272
HpaII	CCGG	8 cut(s)	119, 471, 558, 1127, 1442, 1676, 2124, 2228
HphI	GGTGA	8 cut(s)	547, 696, 1463, 1763, 2210, 2337, 2443, 2474
Hpy166II	GTNNAC	6 cut(s)	298, 405, 916, 1202, 1642, 2201
Hpy188I	TCNGA	7 cut(s)	1145, 1351, 1565, 1735, 2069, 2288, 2376
Hpy8I	GTNNAC	6 cut(s)	298, 405, 916, 1202, 1642, 2201
Hpy99I	CGWCG	1 cut(s)	2292
HpyCH4III	ACNGT	6 cut(s)	185, 691, 859, 964, 1451, 2205
HpyCH4IV	ACGT	4 cut(s)	401, 735, 2304, 2328
HpyF3I	CTNAG	3 cut(s)	333, 652, 1732
HpySE526I	ACGT	4 cut(s)	401, 735, 2304, 2328
Hsp92I	GRCGYC	1 cut(s)	169
HspAI	GCGC	5 cut(s)	44, 468, 978, 1598, 1728
KroI	GCCGGC	1 cut(s)	470
KroNI	GCCGGC	1 cut(s)	472
Kzo9I	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
LguI	GCTCTTC	2 cut(s)	75, 1003
LmnI	GCTCC	6 cut(s)	383, 434, 820, 2069, 2310, 2398
LweI	GCATC	7 cut(s)	516, 703, 749, 1222, 1338, 1752, 2412
MaeI	CTAG	5 cut(s)	41, 1322, 1409, 2078, 2111
MaeII	ACGT	4 cut(s)	401, 735, 2304, 2328
MaeIII	GTNAC	5 cut(s)	731, 785, 964, 2343, 2431
MalI	GATC	9 cut(s)	177, 462, 540, 583, 894, 1308, 2026, 2103, 2367
MboI	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
MflI	RGATCY	4 cut(s)	581, 2024, 2101, 2365
MhlI	GDGCHC	4 cut(s)	826, 1698, 2098, 2156
MlyI	GAGTC	4 cut(s)	1258, 1648, 1726, 1939
MmeI	TCCRAC	2 cut(s)	2311, 2399
MreI	CGCCGGCG	1 cut(s)	470
MroNI	GCCGGC	1 cut(s)	470
MroXI	GAANNNNTTC	2 cut(s)	1470, 2469
MspA1I	CMGCKG	3 cut(s)	656, 749, 2177
MspCI	CTTAAG	1 cut(s)	973
MspI	CCGG	8 cut(s)	119, 471, 558, 1127, 1442, 1676, 2124, 2228
Mva1269I	GAATGC	2 cut(s)	125, 1491
MvaI	CCWGG	8 cut(s)	329, 1053, 1488, 1520, 1651, 1692, 2099, 2106
MvnI	CGCG	3 cut(s)	980, 1547, 1967
NaeI	GCCGGC	1 cut(s)	472
NciI	CCSGG	2 cut(s)	119, 1128
NcoI	CCATGG	1 cut(s)	130
NdeII	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
NgoMIV	GCCGGC	1 cut(s)	470
NlaIV	GGNNCC	6 cut(s)	597, 816, 1500, 1629, 1697, 2103
NmeAIII	GCCGAG	1 cut(s)	617
NmuCI	GTSAC	3 cut(s)	731, 2343, 2431
PagI	TCATGA	1 cut(s)	226
PasI	CCCWGGG	1 cut(s)	1691
PceI	AGGCCT	1 cut(s)	2109
PciSI	GCTCTTC	2 cut(s)	75, 1003
PctI	GAATGC	2 cut(s)	125, 1491
PdiI	GCCGGC	1 cut(s)	472
PdmI	GAANNNNTTC	2 cut(s)	1470, 2469
PfeI	GAWTC	7 cut(s)	221, 480, 500, 603, 1356, 1542, 1614
Pfl23II	CGTACG	1 cut(s)	981
PflFI	GACNNNGTC	1 cut(s)	119
PfoI	TCCNGGA	1 cut(s)	1649
PleI	GAGTC	4 cut(s)	1258, 1648, 1725, 1939
PmaCI	CACGTG	1 cut(s)	736
PmlI	CACGTG	1 cut(s)	736
PpsI	GAGTC	4 cut(s)	1258, 1648, 1725, 1939
Ppu21I	YACGTR	1 cut(s)	736
PpuMI	RGGWCCY	1 cut(s)	596
Psp5II	RGGWCCY	1 cut(s)	596
Psp6I	CCWGG	8 cut(s)	327, 1051, 1486, 1518, 1649, 1690, 2097, 2104
PspCI	CACGTG	1 cut(s)	736
PspEI	GGTNACC	1 cut(s)	964
PspFI	CCCAGC	2 cut(s)	607, 656
PspGI	CCWGG	8 cut(s)	327, 1051, 1486, 1518, 1649, 1690, 2097, 2104
PspLI	CGTACG	1 cut(s)	981
PspN4I	GGNNCC	6 cut(s)	597, 816, 1500, 1629, 1697, 2103
PspPI	GGNCC	8 cut(s)	12, 199, 596, 938, 1049, 1181, 1498, 1628
PspPPI	RGGWCCY	1 cut(s)	596
PsrI	GAACNNNNNNTAC	2 cut(s)	2403, 2435
PstI	CTGCAG	3 cut(s)	355, 1775, 2182
PsuI	RGATCY	4 cut(s)	581, 2024, 2101, 2365
PsyI	GACNNNGTC	1 cut(s)	119
PvuII	CAGCTG	2 cut(s)	656, 2177
RsaI	GTAC	9 cut(s)	95, 404, 983, 1320, 1856, 1879, 1931, 1991, 2041
RsaNI	GTAC	9 cut(s)	94, 403, 982, 1319, 1855, 1878, 1930, 1990, 2040
SapI	GCTCTTC	2 cut(s)	75, 1003
Sau3AI	GATC	9 cut(s)	175, 460, 538, 581, 892, 1306, 2024, 2101, 2365
Sau96I	GGNCC	8 cut(s)	12, 199, 596, 938, 1049, 1181, 1498, 1628
ScaI	AGTACT	1 cut(s)	1320
SchI	GAGTC	4 cut(s)	1258, 1648, 1726, 1939
SduI	GDGCHC	4 cut(s)	826, 1698, 2098, 2156
SfaNI	GCATC	7 cut(s)	516, 703, 749, 1222, 1338, 1752, 2412
SfcI	CTRYAG	8 cut(s)	239, 265, 351, 687, 855, 1089, 1771, 2178
SgrAI	CRCCGGYG	1 cut(s)	470
SinI	GGWCC	4 cut(s)	596, 1181, 1498, 1628
SmlI	CTYRAG	1 cut(s)	973
SmoI	CTYRAG	1 cut(s)	973
SpeI	ACTAGT	1 cut(s)	2077
SseBI	AGGCCT	1 cut(s)	2109
SspI	AATATT	1 cut(s)	33
SspMI	CTAG	5 cut(s)	41, 1322, 1409, 2078, 2111
StuI	AGGCCT	1 cut(s)	2109
StyI	CCWWGG	5 cut(s)	130, 418, 430, 1082, 1259
TaaI	ACNGT	6 cut(s)	185, 691, 859, 964, 1451, 2205
TaiI	ACGT	4 cut(s)	404, 738, 2307, 2331
TaqII	GACCGA	1 cut(s)	1198
TatI	WGTACW	4 cut(s)	93, 1318, 1929, 2039
TauI	GCSGC	1 cut(s)	1550
TfiI	GAWTC	7 cut(s)	221, 480, 500, 603, 1356, 1542, 1614
TscAI	CASTG	8 cut(s)	22, 190, 696, 777, 864, 1242, 1387, 1984
TseFI	GTSAC	3 cut(s)	731, 2343, 2431
Tsp45I	GTSAC	3 cut(s)	731, 2343, 2431
TspDTI	ATGAA	7 cut(s)	606, 960, 1092, 1447, 1576, 1929, 2145
TspGWI	ACGGA	5 cut(s)	177, 810, 922, 1380, 2305
TspRI	CASTG	8 cut(s)	22, 190, 696, 777, 864, 1242, 1387, 1984
Tth111I	GACNNNGTC	1 cut(s)	119
Vha464I	CTTAAG	1 cut(s)	973
VpaK11BI	GGWCC	4 cut(s)	596, 1181, 1498, 1628
XapI	RAATTY	2 cut(s)	413, 1701
XmnI	GAANNNNTTC	2 cut(s)	1470, 2469
XspI	CTAG	5 cut(s)	41, 1322, 1409, 2078, 2111
ZrmI	AGTACT	1 cut(s)	1320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.