Rw2G029690

Belongs to the peptidase S8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006269
Physical Location & Seq
Forward (+)
48143121 .. 48144234
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029690.1

Sequence Viewer

Length: 654 bp
ATGGAAGAAGACTCGGTTGCAATCAATGGAGGCTCTCCATCTTCACGCCTCAACCTAAATAGTGAAGTTTCGAAGGCTCATGTAAAGCAATTGGAGGATTCTCATGGTCAACTTCTACAGAGCAATCTTGAGACCGGAAGCTACAGCAAGCTTTACAGCTTTAAACACGTTGCGAACGGGTTTGCTGTCCATACAACCCCTTCTCAGGTTGAAAAGCTCAAATATGCTCCAGGAGTAAAGCTGGTTGAGAGAGATAGAGGAGCAAAGTTGATGACAACGTACACTCCTCGCTTCCTCGAGTTACCTGAAGGAGTATGGACACAAGAAGGAGGCCAACGTAATGCTGGTGAAGGAATTGTGATTGGTTTTGTGGATAGTGGCATCAATCCTGCACATCTAAGTTTTGCTTATAATCCTTTGAGCCCTTTCACCTCCAATATTTCTCATTTTTCGGGCGCTTGTGAGACTGTTCCTCTATTCCCAGCAACTTCATGCAATGGTAAAATTGTTTCAGCTAAGTTTTTCTCAGCTGGGGCTCGAGCCATTGCTACTCTCAACGCTTCGGTGGATTTTCTTTCACCTTCAACGCAGATGGACATGGCAGTCATGTGGCATCAGTTGCTGCTGGAAATGTTGGAGTTCCTATGGTTGTGA

Protein Analysis

217

Amino Acids

23.61

Weight (kDa)

5.96

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 28 - 90 1.8e-12 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AasI GACNNNNNNGTC 1 cut(s) 602
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 2 cut(s) 212, 585
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 7 cut(s) 141, 151, 159, 217, 241, 515, 530
AluI AGCT 7 cut(s) 141, 151, 159, 217, 241, 515, 530
Alw26I GTCTC 2 cut(s) 125, 458
AlwNI CAGNNNCTG 1 cut(s) 622
Ama87I CYCGRG 2 cut(s) 296, 537
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 1 cut(s) 622
AspLEI GCGC 1 cut(s) 458
AsuHPI GGTGA 3 cut(s) 359, 421, 570
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 2 cut(s) 296, 537
BanII GRGCYC 2 cut(s) 425, 538
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 1 cut(s) 609
BccI CCATC 2 cut(s) 46, 586
BciT130I CCWGG 1 cut(s) 231
BcoDI GTCTC 2 cut(s) 125, 458
BfmI CTRYAG 2 cut(s) 116, 142
BfoI RGCGCY 1 cut(s) 459
BisI GCNGC 1 cut(s) 623
BlsI GCNGC 1 cut(s) 624
Bme1390I CCNGG 1 cut(s) 231
BmeT110I CYCGRG 2 cut(s) 296, 537
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 2 cut(s) 390, 622
BpiI GAAGAC 1 cut(s) 15
BpmI CTGGAG 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 125
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse3DI GCAATG 2 cut(s) 502, 543
BseBI CCWGG 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMI GCAATG 2 cut(s) 502, 543
BseMII CTCAG 2 cut(s) 218, 540
BseRI GAGGAG 2 cut(s) 273, 276
BseXI GCAGC 1 cut(s) 609
BseYI CCCAGC 2 cut(s) 481, 530
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 296, 537
BsiSI CCGG 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 2 cut(s) 125, 458
BsnI GGCC 1 cut(s) 333
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 2 cut(s) 296, 537
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 425, 538
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 2 cut(s) 217, 539
BspT104I TTCGAA 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 502, 543
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 469
BstAPI GCANNNNNTGC 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 4 cut(s) 204, 398, 516, 526
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstMAI GTCTC 2 cut(s) 125, 458
BstMWI GCNNNNNNNGC 1 cut(s) 619
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 229
BstSFI CTRYAG 2 cut(s) 116, 142
BstV1I GCAGC 1 cut(s) 609
BstV2I GAAGAC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 149
CaiI CAGNNNCTG 1 cut(s) 622
CfoI GCGC 1 cut(s) 458
Csp6I GTAC 1 cut(s) 280
CviAII CATG 5 cut(s) 80, 104, 492, 598, 607
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 4 cut(s) 204, 398, 516, 526
DraI TTTAAA 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
Eco24I GRGCYC 2 cut(s) 425, 538
Eco31I GGTCTC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 2 cut(s) 296, 537
EcoRII CCWGG 1 cut(s) 229
EcoT38I GRGCYC 2 cut(s) 425, 538
FaeI CATG 5 cut(s) 83, 107, 495, 601, 610
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FatI CATG 5 cut(s) 79, 103, 491, 597, 606
Fnu4HI GCNGC 1 cut(s) 623
FriOI GRGCYC 2 cut(s) 425, 538
Fsp4HI GCNGC 1 cut(s) 623
GlaI GCGC 1 cut(s) 457
GluI GCNGC 1 cut(s) 623
GsaI CCCAGC 2 cut(s) 485, 534
GsuI CTGGAG 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 1 cut(s) 333
HapII CCGG 1 cut(s) 135
HhaI GCGC 1 cut(s) 458
Hin1II CATG 5 cut(s) 83, 107, 495, 601, 610
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 1 cut(s) 110
HindII GTYRAC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 2 cut(s) 11, 98
HpaII CCGG 1 cut(s) 135
HphI GGTGA 3 cut(s) 359, 421, 570
Hpy166II GTNNAC 2 cut(s) 110, 282
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 2 cut(s) 110, 282
HpyAV CCTTC 6 cut(s) 67, 210, 302, 320, 344, 591
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 3 cut(s) 168, 278, 337
HpyCH4V TGCA 3 cut(s) 20, 392, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 619
HpyF3I CTNAG 4 cut(s) 204, 398, 516, 526
HpySE526I ACGT 3 cut(s) 168, 278, 337
Hsp92II CATG 5 cut(s) 83, 107, 495, 601, 610
HspAI GCGC 1 cut(s) 456
LmnI GCTCC 2 cut(s) 232, 260
Lsp1109I GCAGC 1 cut(s) 609
LweI GCATC 2 cut(s) 390, 622
MaeII ACGT 3 cut(s) 168, 278, 337
MaeIII GTNAC 1 cut(s) 300
MboII GAAGA 3 cut(s) 17, 20, 33
MfeI CAATTG 1 cut(s) 89
MhlI GDGCHC 2 cut(s) 425, 538
MluCI AATT 3 cut(s) 89, 354, 504
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 615
MnlI CCTC 9 cut(s) 23, 59, 88, 251, 297, 305, 323, 442, 483
MseI TTAA 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 1 cut(s) 619
NlaIII CATG 5 cut(s) 83, 107, 495, 601, 610
NspV TTCGAA 1 cut(s) 71
PaeR7I CTCGAG 2 cut(s) 296, 537
PfeI GAWTC 1 cut(s) 98
PfoI TCCNGGA 1 cut(s) 229
PkrI GCNGC 1 cut(s) 624
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PsiI TTATAA 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 2 cut(s) 481, 530
PspGI CCWGG 1 cut(s) 229
PspXI VCTCGAGB 2 cut(s) 296, 537
PstNI CAGNNNCTG 1 cut(s) 622
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 1 cut(s) 623
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 425, 538
SfaNI GCATC 2 cut(s) 390, 622
SfcI CTRYAG 2 cut(s) 116, 142
Sfr274I CTCGAG 2 cut(s) 296, 537
SfuI TTCGAA 1 cut(s) 71
SlaI CTCGAG 2 cut(s) 296, 537
SmlI CTYRAG 3 cut(s) 128, 296, 537
SmoI CTYRAG 3 cut(s) 128, 296, 537
Sse9I AATT 3 cut(s) 89, 354, 504
SspI AATATT 1 cut(s) 439
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 3 cut(s) 171, 281, 340
TaqI TCGA 3 cut(s) 71, 297, 538
TasI AATT 3 cut(s) 89, 354, 504
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TseI GCWGC 1 cut(s) 622
TspDTI ATGAA 1 cut(s) 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XhoI CTCGAG 2 cut(s) 296, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.