MD06G1004000.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001423
Physical Location & Seq
Forward (+)
469887 .. 474673
4787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1004000.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGCGTATCAGAGTTCTGGGTCGAGTTCCAGTCCCTCTGGATTCCAATTCCCCAATTCCAGTTCCCCTTTTGGTGACACCACCTACACCAAGGTCTTTGTTGGGGGGCTCGCCTGGGAGACGCACAGCGACACCATGCGCCGCCATTTCGACCAGTTCGGCCAGATTCTCGAGGCCGTCGTTATTATTGATAAGAACTCCGGCCGATCCAAAGGTTATGGCTTTGTGACTTTCCGCGAACCAGAGGCCGCTGCTCGGGCCTGTGCTGATCCAACTCCAATTATCGATGGCAGGCGCGCTAATTGTAATTTGGCTTCACTCCGGCAGTCCAGGCCACCTATGCCTTATGGACGTCCAAGACCAGCTTCTCCATACATTGGAGGCGTGCAAGCTACCCGTGGGGCTTACATTGGAGGATATGGCTACCAGCAGCCTCTTTCTTACAACTATCAACAAGGATTAGTTTATCCTCCATATGGTGCGTGCAATTCAAAATATGCAATGCCATGTAGATTTCCAAAATCAGGAGACGAAAAAGTGATAGTTGCAGAGGATATGTGCCCAGATCAATGTTTATTGAACAGGCAAGACTTAGGACTAGTGTTTTTTATTGAGGACCAACAATTGGGTTTTCTAGTCTCACCATTATTGATTGATCTCATACTTGAGATTTCTCATTCAAGTATAGGAGCACTTCAGCCTGTAGGTTTATACAACCCCTATGTGGGTCAGCAATATCTTCAAATATACGGACTTCCAGGGACAGCTAATACTTCTCATTATCCTTATGAACAACTGGGTCAAACCATTCCTGGTGGTCAGGGTTATCCAACAGTTCACGGATATGCAATGCCTGCTCAGCAGATAATCCAGTTTGGTGCACCCAGTGTTAATGCAGTCACAACTTCCCCAGTCCCTCAAGTTCAAGCAGCATATCCTGCAGGTATGGTAGCAAGTGTTGTGGCACAACCACATTTTATATTATCTGCTCCTCCTCAATATATGCAGGGTAGTGGTTCTGAGCAGAGAGCTGGGTATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000381 GO:0001501 GO:0001756 GO:0001947 GO:0002009 GO:0003002 GO:0003007 GO:0003143 GO:0003228 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0007368 GO:0007389 GO:0007507 GO:0007517 GO:0007519 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009792 GO:0009799 GO:0009855 GO:0009887 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009896 GO:0009952 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010638 GO:0010720 GO:0010830 GO:0010831 GO:0014706 GO:0016070 GO:0016071 GO:0016202 GO:0017091 GO:0017148 GO:0019219 GO:0019222 GO:0022603 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031330 GO:0031331 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032956 GO:0032970 GO:0033043 GO:0033554 GO:0034248 GO:0034249 GO:0034641 GO:0035050 GO:0035239 GO:0035282 GO:0035295 GO:0035925 GO:0036002 GO:0043009 GO:0043170 GO:0043484 GO:0043487 GO:0043488 GO:0043489 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0045595 GO:0045597 GO:0045661 GO:0045663 GO:0045844 GO:0045934 GO:0045935 GO:0046483 GO:0048024 GO:0048255 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048562 GO:0048568 GO:0048598 GO:0048634 GO:0048636 GO:0048641 GO:0048643 GO:0048646 GO:0048701 GO:0048702 GO:0048704 GO:0048705 GO:0048706 GO:0048729 GO:0048731 GO:0048856 GO:0050684 GO:0050779 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051171 GO:0051172 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051248 GO:0051252 GO:0051253 GO:0051254 GO:0051493 GO:0051495 GO:0051716 GO:0060255 GO:0060284 GO:0060297 GO:0060298 GO:0060429 GO:0060537 GO:0060538 GO:0060562 GO:0061013 GO:0061014 GO:0061053 GO:0061061 GO:0061157 GO:0061158 GO:0061371 GO:0065007 GO:0065008 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097157 GO:0097159 GO:0110020 GO:1901360 GO:1901363 GO:1901861 GO:1901863 GO:1902115 GO:1902117 GO:1902369 GO:1902373 GO:1902809 GO:1902811 GO:1902903 GO:1902905 GO:1903311 GO:1903312 GO:1903313 GO:1904888 GO:1905868 GO:1905870 GO:1990715 GO:1990825 GO:2000026 GO:2000112 GO:2000113 GO:2000736 GO:2000738 GO:2000765 GO:2000766 GO:2001014 GO:2001016
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

348

Amino Acids

37.54

Weight (kDa)

6.58

Isoelectric Point (pI)

59.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 32 - 88 1.7e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 355
Acc36I ACCTGC 1 cut(s) 932
AccB7I CCANNNNNTGG 2 cut(s) 377, 625
AccII CGCG 2 cut(s) 237, 297
AciI CCGC 3 cut(s) 142, 235, 249
AclWI GGATC 2 cut(s) 201, 263
AcoI YGGCCR 2 cut(s) 160, 202
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 352
AdeI CACNNNGTG 1 cut(s) 887
AfiI CCNNNNNNNGG 7 cut(s) 255, 377, 476, 524, 625, 724, 725
AgsI TTSAA 5 cut(s) 492, 580, 681, 743, 926
AhlI ACTAGT 1 cut(s) 598
AjnI CCWGG 4 cut(s) 113, 329, 757, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 349, 381
AluBI AGCT 4 cut(s) 365, 392, 767, 1031
AluI AGCT 4 cut(s) 365, 392, 767, 1031
Alw21I GWGCWC 2 cut(s) 694, 883
Alw26I GTCTC 3 cut(s) 113, 522, 643
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 2 cut(s) 201, 263
Ama87I CYCGRG 2 cut(s) 170, 255
AoxI GGCC 6 cut(s) 160, 174, 202, 246, 258, 332
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 3 cut(s) 251, 430, 929
AspLEI GCGC 3 cut(s) 141, 297, 299
AspS9I GGNCC 2 cut(s) 258, 616
AsuHPI GGTGA 2 cut(s) 86, 633
AvaI CYCGRG 2 cut(s) 170, 255
AvaII GGWCC 1 cut(s) 616
BaeGI GKGCMC 2 cut(s) 563, 883
BanII GRGCYC 1 cut(s) 111
BarI GAAGNNNNNNTAC 2 cut(s) 298, 330
Bbv12I GWGCWC 2 cut(s) 694, 883
BbvI GCAGC 3 cut(s) 238, 442, 941
BccI CCATC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 4 cut(s) 115, 331, 759, 813
BcoDI GTCTC 3 cut(s) 113, 522, 643
BcuI ACTAGT 1 cut(s) 598
BfaI CTAG 2 cut(s) 599, 635
BfmI CTRYAG 2 cut(s) 702, 939
BfuAI ACCTGC 1 cut(s) 932
BisI GCNGC 5 cut(s) 142, 249, 252, 431, 930
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 5 cut(s) 143, 250, 253, 432, 931
Bme1390I CCNGG 4 cut(s) 115, 331, 759, 813
Bme18I GGWCC 1 cut(s) 616
BmeT110I CYCGRG 2 cut(s) 170, 255
BmgT120I GGNCC 2 cut(s) 258, 616
BmrFI CCNGG 4 cut(s) 115, 331, 759, 813
BmrI ACTGGG 3 cut(s) 806, 879, 905
BmuI ACTGGG 3 cut(s) 806, 879, 905
Bpu1102I GCTNAGC 1 cut(s) 858
BpuEI CTTGAG 2 cut(s) 686, 903
Bsa29I ATCGAT 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 352
BsaJI CCNNGG 4 cut(s) 90, 114, 397, 758
Bsc4I CCNNNNNNNGG 7 cut(s) 255, 377, 476, 524, 625, 724, 725
Bse1I ACTGG 7 cut(s) 30, 60, 154, 801, 871, 885, 911
Bse3DI GCAATG 2 cut(s) 507, 855
BseBI CCWGG 4 cut(s) 115, 331, 759, 813
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 4 cut(s) 90, 114, 397, 758
BseLI CCNNNNNNNGG 7 cut(s) 255, 377, 476, 524, 625, 724, 725
BseMI GCAATG 2 cut(s) 507, 855
BseMII CTCAG 2 cut(s) 872, 1011
BseNI ACTGG 7 cut(s) 30, 60, 154, 801, 871, 885, 911
BsePI GCGCGC 1 cut(s) 295
BseRI GAGGAG 2 cut(s) 981, 984
BseSI GKGCMC 2 cut(s) 563, 883
BseX3I CGGCCG 1 cut(s) 202
BseXI GCAGC 3 cut(s) 238, 442, 941
BseYI CCCAGC 1 cut(s) 1031
Bsh1236I CGCG 2 cut(s) 237, 297
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 6 cut(s) 162, 176, 204, 248, 260, 334
BshVI ATCGAT 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 2 cut(s) 694, 883
BsiHKCI CYCGRG 2 cut(s) 170, 255
BsiSI CCGG 2 cut(s) 201, 322
BslFI GGGAC 3 cut(s) 18, 775, 899
BslI CCNNNNNNNGG 7 cut(s) 255, 377, 476, 524, 625, 724, 725
BsmAI GTCTC 3 cut(s) 113, 522, 643
BsmBI CGTCTC 2 cut(s) 113, 522
BsmFI GGGAC 3 cut(s) 18, 775, 899
BsnI GGCC 6 cut(s) 162, 176, 204, 248, 260, 334
BsoBI CYCGRG 2 cut(s) 170, 255
Bsp1286I GDGCHC 4 cut(s) 111, 563, 694, 883
Bsp143I GATC 4 cut(s) 206, 268, 565, 655
Bsp1720I GCTNAGC 1 cut(s) 858
BspACI CCGC 3 cut(s) 142, 235, 249
BspANI GGCC 6 cut(s) 162, 176, 204, 248, 260, 334
BspCNI CTCAG 2 cut(s) 871, 1012
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 2 cut(s) 237, 297
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 2 cut(s) 201, 263
BsrDI GCAATG 2 cut(s) 507, 855
BsrI ACTGG 7 cut(s) 30, 60, 154, 801, 871, 885, 911
BssECI CCNNGG 4 cut(s) 90, 114, 397, 758
BssHII GCGCGC 1 cut(s) 295
BssMI GATC 4 cut(s) 206, 268, 565, 655
BssNI GRCGYC 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 4 cut(s) 115, 331, 759, 813
Bst4CI ACNGT 1 cut(s) 835
BstACI GRCGYC 1 cut(s) 352
BstAPI GCANNNNNTGC 2 cut(s) 854, 938
BstC8I GCNNGC 7 cut(s) 111, 293, 297, 386, 390, 484, 855
BstDEI CTNAG 4 cut(s) 592, 858, 1020, 1041
BstDSI CCRYGG 1 cut(s) 397
BstFNI CGCG 2 cut(s) 237, 297
BstHHI GCGC 3 cut(s) 141, 297, 299
BstKTI GATC 4 cut(s) 209, 271, 568, 658
BstMAI GTCTC 3 cut(s) 113, 522, 643
BstMBI GATC 4 cut(s) 206, 268, 565, 655
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 6 cut(s) 257, 331, 340, 854, 859, 938
BstNI CCWGG 4 cut(s) 115, 331, 759, 813
BstSCI CCNGG 4 cut(s) 113, 329, 757, 811
BstSFI CTRYAG 2 cut(s) 702, 939
BstSLI GKGCMC 2 cut(s) 563, 883
BstUI CGCG 2 cut(s) 237, 297
BstV1I GCAGC 3 cut(s) 238, 442, 941
BstZI CGGCCG 1 cut(s) 202
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 6 cut(s) 162, 176, 204, 248, 260, 334
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 892
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 7 cut(s) 111, 293, 297, 386, 390, 484, 855
CfoI GCGC 3 cut(s) 141, 297, 299
Cfr13I GGNCC 2 cut(s) 258, 616
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 130
CspCI CAANNNNNGTGG 2 cut(s) 942, 977
CviAII CATG 2 cut(s) 136, 507
DdeI CTNAG 4 cut(s) 592, 858, 1020, 1041
DpnI GATC 4 cut(s) 208, 270, 567, 657
DpnII GATC 4 cut(s) 206, 268, 565, 655
DraIII CACNNNGTG 1 cut(s) 887
EaeI YGGCCR 2 cut(s) 160, 202
EagI CGGCCG 1 cut(s) 202
EclXI CGGCCG 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 616
Eco52I CGGCCG 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 680
Eco88I CYCGRG 2 cut(s) 170, 255
EcoRII CCWGG 4 cut(s) 113, 329, 757, 811
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 2 cut(s) 113, 522
FaeI CATG 2 cut(s) 139, 510
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 3 cut(s) 18, 775, 899
FatI CATG 2 cut(s) 135, 506
FauNDI CATATG 1 cut(s) 475
Fnu4HI GCNGC 5 cut(s) 142, 249, 252, 431, 930
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 5 cut(s) 142, 249, 252, 431, 930
FspBI CTAG 2 cut(s) 599, 635
GlaI GCGC 3 cut(s) 140, 296, 298
GluI GCNGC 5 cut(s) 142, 249, 252, 431, 930
GsaI CCCAGC 1 cut(s) 1035
HaeIII GGCC 6 cut(s) 162, 176, 204, 248, 260, 334
HapII CCGG 2 cut(s) 201, 322
HgaI GACGC 1 cut(s) 130
HhaI GCGC 3 cut(s) 141, 297, 299
Hin1I GRCGYC 1 cut(s) 352
Hin1II CATG 2 cut(s) 139, 510
Hin6I GCGC 3 cut(s) 139, 295, 297
HinP1I GCGC 3 cut(s) 139, 295, 297
HinfI GANTC 2 cut(s) 42, 166
HpaII CCGG 2 cut(s) 201, 322
HphI GGTGA 2 cut(s) 86, 633
Hpy166II GTNNAC 2 cut(s) 838, 881
Hpy188I TCNGA 2 cut(s) 12, 1021
Hpy188III TCNNGA 3 cut(s) 39, 170, 525
Hpy8I GTNNAC 2 cut(s) 838, 881
Hpy99I CGWCG 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 835
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 9 cut(s) 388, 486, 500, 548, 848, 881, 896, 941, 1006
HpyF10VI GCNNNNNNNGC 6 cut(s) 257, 331, 340, 854, 859, 938
HpyF3I CTNAG 4 cut(s) 592, 858, 1020, 1041
HpySE526I ACGT 1 cut(s) 352
Hsp92I GRCGYC 1 cut(s) 352
Hsp92II CATG 2 cut(s) 139, 510
HspAI GCGC 3 cut(s) 139, 295, 297
Kzo9I GATC 4 cut(s) 206, 268, 565, 655
LmnI GCTCC 2 cut(s) 689, 994
Lsp1109I GCAGC 3 cut(s) 238, 442, 941
MaeI CTAG 2 cut(s) 599, 635
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 3 cut(s) 74, 226, 898
MalI GATC 4 cut(s) 208, 270, 567, 657
MboI GATC 4 cut(s) 206, 268, 565, 655
MboII GAAGA 1 cut(s) 731
MfeI CAATTG 1 cut(s) 623
MhlI GDGCHC 4 cut(s) 111, 563, 694, 883
MluCI AATT 7 cut(s) 47, 55, 279, 301, 307, 487, 623
MmeI TCCRAC 2 cut(s) 296, 854
MseI TTAA 1 cut(s) 891
MslI CAYNNNNRTG 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 2 cut(s) 201, 322
MspR9I CCNGG 4 cut(s) 115, 331, 759, 813
MunI CAATTG 1 cut(s) 623
MvaI CCWGG 4 cut(s) 115, 331, 759, 813
MvnI CGCG 2 cut(s) 237, 297
MwoI GCNNNNNNNGC 6 cut(s) 257, 331, 340, 854, 859, 938
NdeI CATATG 1 cut(s) 475
NdeII GATC 4 cut(s) 206, 268, 565, 655
NlaIII CATG 2 cut(s) 139, 510
NmuCI GTSAC 3 cut(s) 74, 226, 898
PaeR7I CTCGAG 1 cut(s) 170
PauI GCGCGC 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 2 cut(s) 42, 166
PflMI CCANNNNNTGG 2 cut(s) 377, 625
PkrI GCNGC 5 cut(s) 143, 250, 253, 432, 931
Psp6I CCWGG 4 cut(s) 113, 329, 757, 811
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 4 cut(s) 113, 329, 757, 811
PspPI GGNCC 2 cut(s) 258, 616
PstI CTGCAG 1 cut(s) 943
PteI GCGCGC 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 843
SaqAI TTAA 1 cut(s) 891
SatI GCNGC 5 cut(s) 142, 249, 252, 431, 930
Sau3AI GATC 4 cut(s) 206, 268, 565, 655
Sau96I GGNCC 2 cut(s) 258, 616
SbfI CCTGCAGG 1 cut(s) 943
ScrFI CCNGG 4 cut(s) 115, 331, 759, 813
SdaI CCTGCAGG 1 cut(s) 943
SduI GDGCHC 4 cut(s) 111, 563, 694, 883
SfcI CTRYAG 2 cut(s) 702, 939
Sfr274I CTCGAG 1 cut(s) 170
SinI GGWCC 1 cut(s) 616
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 3 cut(s) 170, 665, 918
SmoI CTYRAG 3 cut(s) 170, 665, 918
SpeI ACTAGT 1 cut(s) 598
Sse8387I CCTGCAGG 1 cut(s) 943
Sse9I AATT 7 cut(s) 47, 55, 279, 301, 307, 487, 623
SsiI CCGC 3 cut(s) 142, 235, 249
SspMI CTAG 2 cut(s) 599, 635
StyD4I CCNGG 4 cut(s) 113, 329, 757, 811
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 835
TaiI ACGT 1 cut(s) 355
TaqI TCGA 4 cut(s) 23, 150, 171, 285
TasI AATT 7 cut(s) 47, 55, 279, 301, 307, 487, 623
TauI GCSGC 2 cut(s) 144, 251
TfiI GAWTC 2 cut(s) 42, 166
Tru1I TTAA 1 cut(s) 891
Tru9I TTAA 1 cut(s) 891
TscAI CASTG 1 cut(s) 892
TseFI GTSAC 3 cut(s) 74, 226, 898
TseI GCWGC 3 cut(s) 251, 430, 929
Tsp45I GTSAC 3 cut(s) 74, 226, 898
TspDTI ATGAA 1 cut(s) 804
TspGWI ACGGA 2 cut(s) 765, 855
TspRI CASTG 1 cut(s) 892
Van91I CCANNNNNTGG 2 cut(s) 377, 625
VneI GTGCAC 1 cut(s) 879
VpaK11BI GGWCC 1 cut(s) 616
XhoI CTCGAG 1 cut(s) 170
XspI CTAG 2 cut(s) 599, 635
ZraI GACGTC 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.