pycom15g20280

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001423
Physical Location & Seq
Forward (+)
14486265 .. 14488361
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g20280.1

Sequence Viewer

Length: 852 bp
ATGGCTTACCACCAGCAGCAGCAGCAGCAACAGCAAGGAGGTGGGCAGCTGTTCAATTCCCCGTTCGGGGACACGACGTTGACGAAGGTGTTCGTGGGGGGGCTGGCCTGGGAGACCCAGAGCGACACTCTACGACGTCACTTCGAGCAGTTCGGCGACATCCTCGAGGCCGTCGTCATCACCGATAAGAACACCGCCCGATCCAAAGGCTATGGCTTTGTCACATTTCGTGAACCTGAATCTGCAAGAAGGGCTTGTATGGATCCCAATCCTGTGATCGATGGCCGCAGAGCCAACTGCAATCTTGCCTCTCTGGGTCGTCCCCGCCAACCTTTCCCTTTTCATGGGCAATTGAGATCACCAGCTCCAATCCTCGGTCGTGTGCAACCGCCTCGAGCTGCTTACATGGGAAGCCCCAACCCTGCTTACCACCACCCTATTCCTCATGGTTACCAGCCCGGGTTCGTGTACCCATCTTATGGGTACACAGGATATGGACCTGAATTTGTCTATCCACAGGGTGCCTACAGTCCTTACATGACTCAGCAGCAGTACCTTCAGATATATGGGGTACCAGGCACTGTTAACCCTGGCGTATATTCATATGGACAATTGAGTCATTCACTCCCCGGGAGTCATGGGTATGCAACGGTTCAAAACTATGGAGTTCCAGGTCATCATGTAATGCAATTTGGCGGCTCCAATGTTGGTGCTGCATCCCCGGCTGCCATTCCAACGATTCAAACACCATATCCTGCAGGTATAATTGCACCAGCTCCAGGACACACGCAGATTATGCTTCCTACTAATTCTCCTCAATTCACCAGTGGTTCCGATCAAACTGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

284

Amino Acids

30.67

Weight (kDa)

8.79

Isoelectric Point (pI)

58.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 30 - 85 3.7e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 615
AatII GACGTC 1 cut(s) 139
AbsI CCTCGAGG 1 cut(s) 164
Acc36I ACCTGC 2 cut(s) 749, 836
Acc65I GGTACC 1 cut(s) 571
AccB1I GGYRCC 2 cut(s) 521, 571
AccB7I CCANNNNNTGG 1 cut(s) 479
AciI CCGC 5 cut(s) 195, 286, 325, 389, 696
AclWI GGATC 3 cut(s) 195, 257, 270
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 503
AcuI CTGAAG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 136
AdeI CACNNNGTG 1 cut(s) 521
AfaI GTAC 4 cut(s) 470, 485, 554, 573
AfiI CCNNNNNNNGG 6 cut(s) 66, 67, 344, 374, 479, 779
AgsI TTSAA 3 cut(s) 55, 656, 743
AjnI CCWGG 5 cut(s) 107, 574, 589, 670, 778
AluBI AGCT 4 cut(s) 49, 365, 398, 776
AluI AGCT 4 cut(s) 49, 365, 398, 776
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 3 cut(s) 195, 257, 270
AlwNI CAGNNNCTG 1 cut(s) 581
Ama87I CYCGRG 4 cut(s) 164, 393, 458, 629
AoxI GGCC 3 cut(s) 105, 168, 283
ApeKI GCWGC 9 cut(s) 16, 19, 22, 25, 46, 398, 547, 713, 725
ApoI RAATTY 1 cut(s) 503
Asp700I GAANNNNTTC 1 cut(s) 89
Asp718I GGTACC 1 cut(s) 571
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 5 cut(s) 459, 460, 630, 631, 722
AsuHPI GGTGA 3 cut(s) 172, 351, 814
AvaI CYCGRG 4 cut(s) 164, 393, 458, 629
AvaII GGWCC 1 cut(s) 497
BamHI GGATCC 1 cut(s) 262
BanI GGYRCC 2 cut(s) 521, 571
BbvI GCAGC 9 cut(s) 28, 31, 34, 37, 58, 385, 559, 700, 712
BccI CCATC 2 cut(s) 275, 481
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 3 cut(s) 374, 408, 824
BciT130I CCWGG 5 cut(s) 109, 576, 591, 672, 780
BcnI CCSGG 5 cut(s) 459, 460, 630, 631, 722
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 3 cut(s) 526, 756, 843
BfuAI ACCTGC 2 cut(s) 749, 836
Bme18I GGWCC 1 cut(s) 497
BmeT110I CYCGRG 4 cut(s) 164, 393, 458, 629
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 5 cut(s) 264, 523, 573, 700, 832
BmsI GCATC 1 cut(s) 725
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpmI CTGGAG 1 cut(s) 762
BpuMI CCSGG 5 cut(s) 459, 460, 630, 631, 722
Bsa29I ATCGAT 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 7 cut(s) 108, 373, 458, 589, 628, 629, 720
BsaXI ACNNNNNCTCC 2 cut(s) 796, 826
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 67, 344, 374, 479, 779
Bse1I ACTGG 1 cut(s) 825
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 5 cut(s) 109, 576, 591, 672, 780
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 7 cut(s) 108, 373, 458, 589, 628, 629, 720
BseGI GGATG 2 cut(s) 159, 716
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 6 cut(s) 66, 67, 344, 374, 479, 779
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 1 cut(s) 825
BseRI GAGGAG 1 cut(s) 804
BseXI GCAGC 9 cut(s) 28, 31, 34, 37, 58, 385, 559, 700, 712
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 3 cut(s) 107, 170, 285
BshNI GGYRCC 2 cut(s) 521, 571
BshVI ATCGAT 1 cut(s) 279
BsiEI CGRYCG 1 cut(s) 379
BsiHKCI CYCGRG 4 cut(s) 164, 393, 458, 629
BsiSI CCGG 3 cut(s) 459, 630, 722
BslFI GGGAC 2 cut(s) 83, 306
BslI CCNNNNNNNGG 6 cut(s) 66, 67, 344, 374, 479, 779
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 2 cut(s) 83, 306
BsnI GGCC 3 cut(s) 107, 170, 285
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 4 cut(s) 164, 393, 458, 629
Bsp143I GATC 5 cut(s) 200, 262, 276, 356, 835
BspACI CCGC 5 cut(s) 195, 286, 325, 389, 696
BspANI GGCC 3 cut(s) 107, 170, 285
BspCNI CTCAG 1 cut(s) 556
BspDI ATCGAT 1 cut(s) 279
BspLI GGNNCC 5 cut(s) 264, 523, 573, 700, 832
BspMAI CTGCAG 2 cut(s) 760, 847
BspMI ACCTGC 2 cut(s) 749, 836
BspPI GGATC 3 cut(s) 195, 257, 270
BspT107I GGYRCC 2 cut(s) 521, 571
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 825
BssECI CCNNGG 7 cut(s) 108, 373, 458, 589, 628, 629, 720
BssMI GATC 5 cut(s) 200, 262, 276, 356, 835
BssNI GRCGYC 1 cut(s) 136
Bst2UI CCWGG 5 cut(s) 109, 576, 591, 672, 780
Bst4CI ACNGT 3 cut(s) 530, 583, 652
BstACI GRCGYC 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 796
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 1 cut(s) 543
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 2 cut(s) 159, 716
BstKTI GATC 5 cut(s) 203, 265, 279, 359, 838
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 5 cut(s) 200, 262, 276, 356, 835
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 6 cut(s) 22, 25, 31, 251, 722, 796
BstNI CCWGG 5 cut(s) 109, 576, 591, 672, 780
BstPI GGTNACC 1 cut(s) 449
BstSFI CTRYAG 3 cut(s) 526, 756, 843
BstV1I GCAGC 9 cut(s) 28, 31, 34, 37, 58, 385, 559, 700, 712
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 3 cut(s) 107, 170, 285
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 2 cut(s) 159, 716
BtsIMutI CAGTG 2 cut(s) 579, 832
BveI ACCTGC 2 cut(s) 749, 836
Cac8I GCNNGC 1 cut(s) 105
CaiI CAGNNNCTG 1 cut(s) 581
Cfr13I GGNCC 1 cut(s) 497
Cfr9I CCCGGG 2 cut(s) 458, 629
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 4 cut(s) 469, 484, 553, 572
CviAII CATG 6 cut(s) 344, 406, 446, 538, 638, 680
CviQI GTAC 4 cut(s) 469, 484, 553, 572
DdeI CTNAG 1 cut(s) 543
DpnI GATC 5 cut(s) 202, 264, 278, 358, 837
DpnII GATC 5 cut(s) 200, 262, 276, 356, 835
DraIII CACNNNGTG 1 cut(s) 521
DrdI GACNNNNNNGTC 1 cut(s) 615
DseDI GACNNNNNNGTC 1 cut(s) 615
EaeI YGGCCR 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 497
Eco57I CTGAAG 1 cut(s) 542
Eco88I CYCGRG 4 cut(s) 164, 393, 458, 629
Eco91I GGTNACC 1 cut(s) 449
EcoO65I GGTNACC 1 cut(s) 449
EcoRII CCWGG 5 cut(s) 107, 574, 589, 670, 778
FaeI CATG 6 cut(s) 347, 409, 449, 541, 641, 683
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 83, 306
FatI CATG 6 cut(s) 343, 405, 445, 537, 637, 679
FauI CCCGC 1 cut(s) 332
FauNDI CATATG 1 cut(s) 604
FokI GGATG 2 cut(s) 146, 703
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 3 cut(s) 107, 170, 285
HapII CCGG 3 cut(s) 459, 630, 722
Hin1I GRCGYC 1 cut(s) 136
Hin1II CATG 6 cut(s) 347, 409, 449, 541, 641, 683
HincII GTYRAC 2 cut(s) 81, 586
HindII GTYRAC 2 cut(s) 81, 586
HinfI GANTC 5 cut(s) 239, 541, 616, 634, 739
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 3 cut(s) 459, 630, 722
HphI GGTGA 3 cut(s) 172, 351, 814
Hpy166II GTNNAC 5 cut(s) 81, 233, 469, 486, 586
Hpy188I TCNGA 2 cut(s) 561, 835
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 5 cut(s) 81, 233, 469, 486, 586
Hpy99I CGWCG 3 cut(s) 79, 138, 176
HpyAV CCTTC 3 cut(s) 79, 243, 566
HpyCH4III ACNGT 3 cut(s) 530, 583, 652
HpyCH4IV ACGT 2 cut(s) 77, 136
HpyCH4V TGCA 9 cut(s) 245, 300, 385, 647, 688, 716, 758, 770, 845
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 25, 31, 251, 722, 796
HpyF3I CTNAG 1 cut(s) 543
HpySE526I ACGT 2 cut(s) 77, 136
Hsp92I GRCGYC 1 cut(s) 136
Hsp92II CATG 6 cut(s) 347, 409, 449, 541, 641, 683
KpnI GGTACC 1 cut(s) 575
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 5 cut(s) 200, 262, 276, 356, 835
LmnI GCTCC 3 cut(s) 370, 704, 781
Lsp1109I GCAGC 9 cut(s) 28, 31, 34, 37, 58, 385, 559, 700, 712
LweI GCATC 1 cut(s) 725
MaeII ACGT 2 cut(s) 77, 136
MaeIII GTNAC 3 cut(s) 137, 220, 449
MalI GATC 5 cut(s) 202, 264, 278, 358, 837
MboI GATC 5 cut(s) 200, 262, 276, 356, 835
MfeI CAATTG 2 cut(s) 350, 611
MflI RGATCY 1 cut(s) 262
MluCI AATT 8 cut(s) 55, 350, 503, 611, 689, 765, 808, 818
MlyI GAGTC 3 cut(s) 535, 625, 643
MmeI TCCRAC 1 cut(s) 758
MnlI CCTC 8 cut(s) 32, 160, 173, 319, 383, 402, 453, 825
MroXI GAANNNNTTC 1 cut(s) 89
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 3 cut(s) 459, 630, 722
MunI CAATTG 2 cut(s) 350, 611
MvaI CCWGG 5 cut(s) 109, 576, 591, 672, 780
MwoI GCNNNNNNNGC 6 cut(s) 22, 25, 31, 251, 722, 796
NciI CCSGG 5 cut(s) 459, 460, 630, 631, 722
NdeI CATATG 1 cut(s) 604
NdeII GATC 5 cut(s) 200, 262, 276, 356, 835
NlaIII CATG 6 cut(s) 347, 409, 449, 541, 641, 683
NlaIV GGNNCC 5 cut(s) 264, 523, 573, 700, 832
NmuCI GTSAC 2 cut(s) 137, 220
PaeR7I CTCGAG 2 cut(s) 164, 393
PcsI WCGNNNNNNNCGW 3 cut(s) 80, 171, 180
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 2 cut(s) 239, 739
PflMI CCANNNNNTGG 1 cut(s) 479
PfoI TCCNGGA 1 cut(s) 778
PleI GAGTC 3 cut(s) 535, 624, 642
PpsI GAGTC 3 cut(s) 535, 624, 642
Psp6I CCWGG 5 cut(s) 107, 574, 589, 670, 778
PspEI GGTNACC 1 cut(s) 449
PspGI CCWGG 5 cut(s) 107, 574, 589, 670, 778
PspN4I GGNNCC 5 cut(s) 264, 523, 573, 700, 832
PspPI GGNCC 1 cut(s) 497
PspXI VCTCGAGB 2 cut(s) 164, 393
PstI CTGCAG 2 cut(s) 760, 847
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 4 cut(s) 470, 485, 554, 573
RsaNI GTAC 4 cut(s) 469, 484, 553, 572
RseI CAYNNNNRTG 1 cut(s) 642
SaqAI TTAA 1 cut(s) 585
Sau3AI GATC 5 cut(s) 200, 262, 276, 356, 835
Sau96I GGNCC 1 cut(s) 497
SbfI CCTGCAGG 1 cut(s) 760
SchI GAGTC 3 cut(s) 535, 625, 643
SdaI CCTGCAGG 1 cut(s) 760
SfaNI GCATC 1 cut(s) 725
SfcI CTRYAG 3 cut(s) 526, 756, 843
Sfr274I CTCGAG 2 cut(s) 164, 393
SinI GGWCC 1 cut(s) 497
SlaI CTCGAG 2 cut(s) 164, 393
SmaI CCCGGG 2 cut(s) 460, 631
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 164, 393
SmoI CTYRAG 2 cut(s) 164, 393
Sse8387I CCTGCAGG 1 cut(s) 760
Sse9I AATT 8 cut(s) 55, 350, 503, 611, 689, 765, 808, 818
SsiI CCGC 5 cut(s) 195, 286, 325, 389, 696
TaaI ACNGT 3 cut(s) 530, 583, 652
TaiI ACGT 2 cut(s) 80, 139
TaqI TCGA 4 cut(s) 144, 165, 279, 394
TaqII GACCGA 1 cut(s) 365
TasI AATT 8 cut(s) 55, 350, 503, 611, 689, 765, 808, 818
TauI GCSGC 2 cut(s) 288, 699
TfiI GAWTC 2 cut(s) 239, 739
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 2 cut(s) 586, 832
TseFI GTSAC 2 cut(s) 137, 220
TseI GCWGC 9 cut(s) 16, 19, 22, 25, 46, 398, 547, 713, 725
Tsp45I GTSAC 2 cut(s) 137, 220
TspDTI ATGAA 2 cut(s) 332, 591
TspMI CCCGGG 2 cut(s) 458, 629
TspRI CASTG 2 cut(s) 586, 832
Van91I CCANNNNNTGG 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 497
XapI RAATTY 1 cut(s) 503
XhoI CTCGAG 2 cut(s) 164, 393
XmaI CCCGGG 2 cut(s) 458, 629
XmnI GAANNNNTTC 1 cut(s) 89
ZraI GACGTC 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.