MD15G1228000.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001423
Physical Location & Seq
Forward (+)
18577110 .. 18579188
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1228000.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGCTTACCAGCAACAGCAAGGAGGTGGGCAGCTGTTCAATTCCCCGTTCGGGGACACGACGTTGACGAAGGTGTTCGTGGGGGGGCTGGCCTGGGAGACCCAGAGCGACACTCTACGACGTCACTTCGAGCAGTTCGGCGACATCCTCGAGGCCGTCGTCATCACCGATAAGAACACCGCCCGATCCAAAGGCTATGGCTTTGTCACATTTCGTGATCCCGAATCTGCAAGAAGGGCTTGTTTGGATCCCAATCCTGTGATCGATGGCCGCAGAGCCAACTGCAATCTTGCTTCTCTGGGTCGTCCCCGCCAACCTTTCCCTTTTCATGGGCAAGTGAGATCACCAGCTCCAATCCTCGGTCGTGTGCAACCGCCTCGAGCTGCTTACATGGGAAGCCCCAACCCTGCTTACCACCACCCTATTCCTCATGGTTACCAGCCCGGGTTCGTGTACCCATCTTATGGGTACACAGGATATGGACCTGAATTTGTCTATCCACAGGGTGCCTACAGTCCTTACATGACTCAGCAGCAGTACCTTCAGATATATGGGGTACCAGGCACCGTTAACCCTGGCATATATTCATATGGACAATTGGGTCATTCACTCCCCGGGAGTCATGGGTATGCAACGGTTCAAAACTATGGAGTTCCAGGTCATCATGTAATGCAATTTGGCGGCTCCAATGTTGGTGCTGCATCCCCAGCTGCCATTCCGACGATTCAAACACCATATCCTGCAGGCAACATTCTCTTCCCAAAACCCTTATTCCCATTTACAAAGATGATAACACACCTTCATTGCAATTTAGTAACTTTATCAGTATTTTCTCTTGAAAATCTTAAATTTTTCCTGATTTATGGTAACTCATATTGTACGCTATGCCTGCGAACCCTATTTCTTTTTTCTGCAAAACATGTATATGATCATACAACATTGCCGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

317

Amino Acids

34.88

Weight (kDa)

9.1

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 25 - 80 1.3e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 600
AatII GACGTC 1 cut(s) 124
AbsI CCTCGAGG 1 cut(s) 149
Acc65I GGTACC 1 cut(s) 556
AccB1I GGYRCC 3 cut(s) 506, 556, 563
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 6 cut(s) 180, 271, 310, 374, 681, 944
AclWI GGATC 4 cut(s) 180, 212, 242, 255
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 2 cut(s) 488, 848
AcuI CTGAAG 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 121
AdeI CACNNNGTG 1 cut(s) 506
AfaI GTAC 5 cut(s) 455, 470, 539, 558, 880
AfiI CCNNNNNNNGG 5 cut(s) 51, 52, 329, 359, 464
AflIII ACRYGT 1 cut(s) 919
AgsI TTSAA 4 cut(s) 40, 641, 728, 839
AjnI CCWGG 4 cut(s) 92, 559, 574, 655
AluBI AGCT 4 cut(s) 34, 350, 383, 710
AluI AGCT 4 cut(s) 34, 350, 383, 710
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 4 cut(s) 180, 212, 242, 255
Ama87I CYCGRG 4 cut(s) 149, 378, 443, 614
AoxI GGCC 3 cut(s) 90, 153, 268
ApeKI GCWGC 5 cut(s) 31, 383, 532, 698, 710
ApoI RAATTY 2 cut(s) 488, 848
Asp700I GAANNNNTTC 1 cut(s) 74
Asp718I GGTACC 1 cut(s) 556
AspS9I GGNCC 1 cut(s) 482
AsuC2I CCSGG 4 cut(s) 444, 445, 615, 616
AsuHPI GGTGA 2 cut(s) 157, 336
AvaI CYCGRG 4 cut(s) 149, 378, 443, 614
AvaII GGWCC 1 cut(s) 482
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 3 cut(s) 506, 556, 563
BbvI GCAGC 5 cut(s) 43, 370, 544, 685, 697
BccI CCATC 2 cut(s) 260, 466
BceAI ACGGC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 4 cut(s) 94, 561, 576, 657
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 4 cut(s) 444, 445, 615, 616
BcoDI GTCTC 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 511, 741
BisI GCNGC 8 cut(s) 32, 271, 384, 533, 682, 699, 711, 944
BlsI GCNGC 8 cut(s) 33, 272, 385, 534, 683, 700, 712, 945
Bme1390I CCNGG 8 cut(s) 94, 444, 445, 561, 576, 615, 616, 657
Bme18I GGWCC 1 cut(s) 482
BmeT110I CYCGRG 4 cut(s) 149, 378, 443, 614
BmgT120I GGNCC 1 cut(s) 482
BmiI GGNNCC 5 cut(s) 249, 508, 558, 565, 685
BmrFI CCNGG 8 cut(s) 94, 444, 445, 561, 576, 615, 616, 657
BmsI GCATC 1 cut(s) 710
BplI GAGNNNNNCTC 2 cut(s) 97, 129
BpuMI CCSGG 4 cut(s) 444, 445, 615, 616
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 6 cut(s) 93, 358, 443, 574, 613, 614
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 52, 329, 359, 464
Bse3DI GCAATG 2 cut(s) 802, 938
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 4 cut(s) 94, 561, 576, 657
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 6 cut(s) 93, 358, 443, 574, 613, 614
BseGI GGATG 2 cut(s) 144, 701
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 5 cut(s) 51, 52, 329, 359, 464
BseMI GCAATG 2 cut(s) 802, 938
BseMII CTCAG 1 cut(s) 542
BseXI GCAGC 5 cut(s) 43, 370, 544, 685, 697
BseYI CCCAGC 1 cut(s) 706
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 3 cut(s) 92, 155, 270
BshNI GGYRCC 3 cut(s) 506, 556, 563
BshVI ATCGAT 1 cut(s) 264
BsiEI CGRYCG 1 cut(s) 364
BsiHKCI CYCGRG 4 cut(s) 149, 378, 443, 614
BsiSI CCGG 2 cut(s) 444, 615
BslFI GGGAC 2 cut(s) 68, 291
BslI CCNNNNNNNGG 5 cut(s) 51, 52, 329, 359, 464
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 68, 291
BsnI GGCC 3 cut(s) 92, 155, 270
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 4 cut(s) 149, 378, 443, 614
Bsp143I GATC 6 cut(s) 185, 217, 247, 261, 341, 928
BspACI CCGC 6 cut(s) 180, 271, 310, 374, 681, 944
BspANI GGCC 3 cut(s) 92, 155, 270
BspCNI CTCAG 1 cut(s) 541
BspDI ATCGAT 1 cut(s) 264
BspLI GGNNCC 5 cut(s) 249, 508, 558, 565, 685
BspMAI CTGCAG 1 cut(s) 745
BspPI GGATC 4 cut(s) 180, 212, 242, 255
BspT107I GGYRCC 3 cut(s) 506, 556, 563
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 802, 938
BssECI CCNNGG 6 cut(s) 93, 358, 443, 574, 613, 614
BssMI GATC 6 cut(s) 185, 217, 247, 261, 341, 928
BssNI GRCGYC 1 cut(s) 121
Bst2UI CCWGG 4 cut(s) 94, 561, 576, 657
Bst4CI ACNGT 3 cut(s) 515, 568, 637
Bst6I CTCTTC 1 cut(s) 761
BstACI GRCGYC 1 cut(s) 121
BstC8I GCNNGC 3 cut(s) 90, 745, 890
BstDEI CTNAG 1 cut(s) 528
BstEII GGTNACC 1 cut(s) 434
BstF5I GGATG 2 cut(s) 144, 701
BstKTI GATC 6 cut(s) 188, 220, 250, 264, 344, 931
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 6 cut(s) 185, 217, 247, 261, 341, 928
BstMCI CGRYCG 1 cut(s) 364
BstMWI GCNNNNNNNGC 3 cut(s) 236, 707, 889
BstNI CCWGG 4 cut(s) 94, 561, 576, 657
BstNSI RCATGY 1 cut(s) 923
BstPI GGTNACC 1 cut(s) 434
BstSCI CCNGG 8 cut(s) 92, 442, 443, 559, 574, 613, 614, 655
BstSFI CTRYAG 2 cut(s) 511, 741
BstV1I GCAGC 5 cut(s) 43, 370, 544, 685, 697
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 3 cut(s) 92, 155, 270
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 2 cut(s) 144, 701
Cac8I GCNNGC 3 cut(s) 90, 745, 890
Cfr13I GGNCC 1 cut(s) 482
Cfr9I CCCGGG 2 cut(s) 443, 614
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 5 cut(s) 454, 469, 538, 557, 879
CviAII CATG 7 cut(s) 329, 391, 431, 523, 623, 665, 920
CviQI GTAC 5 cut(s) 454, 469, 538, 557, 879
DdeI CTNAG 1 cut(s) 528
DpnI GATC 6 cut(s) 187, 219, 249, 263, 343, 930
DpnII GATC 6 cut(s) 185, 217, 247, 261, 341, 928
DraIII CACNNNGTG 1 cut(s) 506
DrdI GACNNNNNNGTC 1 cut(s) 600
DseDI GACNNNNNNGTC 1 cut(s) 600
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 761
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 482
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 4 cut(s) 149, 378, 443, 614
Eco91I GGTNACC 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 434
EcoRII CCWGG 4 cut(s) 92, 559, 574, 655
FaeI CATG 7 cut(s) 332, 394, 434, 526, 626, 668, 923
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FaqI GGGAC 2 cut(s) 68, 291
FatI CATG 7 cut(s) 328, 390, 430, 522, 622, 664, 919
FauI CCCGC 1 cut(s) 317
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 1 cut(s) 928
Fnu4HI GCNGC 8 cut(s) 32, 271, 384, 533, 682, 699, 711, 944
FokI GGATG 2 cut(s) 131, 688
Fsp4HI GCNGC 8 cut(s) 32, 271, 384, 533, 682, 699, 711, 944
GluI GCNGC 8 cut(s) 32, 271, 384, 533, 682, 699, 711, 944
GsaI CCCAGC 1 cut(s) 710
HaeIII GGCC 3 cut(s) 92, 155, 270
HapII CCGG 2 cut(s) 444, 615
Hin1I GRCGYC 1 cut(s) 121
Hin1II CATG 7 cut(s) 332, 394, 434, 526, 626, 668, 923
HincII GTYRAC 2 cut(s) 66, 571
HindII GTYRAC 2 cut(s) 66, 571
HinfI GANTC 4 cut(s) 224, 526, 619, 724
HpaI GTTAAC 1 cut(s) 571
HpaII CCGG 2 cut(s) 444, 615
HphI GGTGA 2 cut(s) 157, 336
Hpy166II GTNNAC 4 cut(s) 66, 454, 471, 571
Hpy188I TCNGA 2 cut(s) 546, 720
Hpy188III TCNNGA 4 cut(s) 215, 221, 836, 856
Hpy8I GTNNAC 4 cut(s) 66, 454, 471, 571
Hpy99I CGWCG 4 cut(s) 64, 123, 161, 724
HpyAV CCTTC 4 cut(s) 64, 228, 551, 809
HpyCH4III ACNGT 3 cut(s) 515, 568, 637
HpyCH4IV ACGT 2 cut(s) 62, 121
HpyCH4V TGCA 9 cut(s) 230, 285, 370, 632, 673, 701, 743, 807, 914
HpyF10VI GCNNNNNNNGC 3 cut(s) 236, 707, 889
HpyF3I CTNAG 1 cut(s) 528
HpySE526I ACGT 2 cut(s) 62, 121
Hsp92I GRCGYC 1 cut(s) 121
Hsp92II CATG 7 cut(s) 332, 394, 434, 526, 626, 668, 923
KpnI GGTACC 1 cut(s) 560
Ksp22I TGATCA 1 cut(s) 928
KspAI GTTAAC 1 cut(s) 571
Kzo9I GATC 6 cut(s) 185, 217, 247, 261, 341, 928
LmnI GCTCC 2 cut(s) 355, 689
Lsp1109I GCAGC 5 cut(s) 43, 370, 544, 685, 697
LweI GCATC 1 cut(s) 710
MaeII ACGT 2 cut(s) 62, 121
MaeIII GTNAC 5 cut(s) 122, 205, 434, 814, 866
MalI GATC 6 cut(s) 187, 219, 249, 263, 343, 930
MboI GATC 6 cut(s) 185, 217, 247, 261, 341, 928
MboII GAAGA 1 cut(s) 748
MfeI CAATTG 1 cut(s) 596
MflI RGATCY 1 cut(s) 247
MluCI AATT 6 cut(s) 40, 488, 596, 674, 808, 848
MlyI GAGTC 2 cut(s) 520, 628
MmeI TCCRAC 1 cut(s) 743
MnlI CCTC 6 cut(s) 17, 145, 158, 368, 387, 438
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 2 cut(s) 570, 846
MslI CAYNNNNRTG 2 cut(s) 627, 924
MspA1I CMGCKG 2 cut(s) 34, 710
MspI CCGG 2 cut(s) 444, 615
MspR9I CCNGG 8 cut(s) 94, 444, 445, 561, 576, 615, 616, 657
MunI CAATTG 1 cut(s) 596
MvaI CCWGG 4 cut(s) 94, 561, 576, 657
MwoI GCNNNNNNNGC 3 cut(s) 236, 707, 889
NciI CCSGG 4 cut(s) 444, 445, 615, 616
NdeI CATATG 1 cut(s) 589
NdeII GATC 6 cut(s) 185, 217, 247, 261, 341, 928
NlaIII CATG 7 cut(s) 332, 394, 434, 526, 626, 668, 923
NlaIV GGNNCC 5 cut(s) 249, 508, 558, 565, 685
NmuCI GTSAC 2 cut(s) 122, 205
NspI RCATGY 1 cut(s) 923
PaeR7I CTCGAG 2 cut(s) 149, 378
PciI ACATGT 1 cut(s) 919
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 156, 165
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 224, 724
PflMI CCANNNNNTGG 1 cut(s) 464
PkrI GCNGC 8 cut(s) 33, 272, 385, 534, 683, 700, 712, 945
PleI GAGTC 2 cut(s) 520, 627
PpsI GAGTC 2 cut(s) 520, 627
PscI ACATGT 1 cut(s) 919
Psp6I CCWGG 4 cut(s) 92, 559, 574, 655
PspEI GGTNACC 1 cut(s) 434
PspFI CCCAGC 1 cut(s) 706
PspGI CCWGG 4 cut(s) 92, 559, 574, 655
PspN4I GGNNCC 5 cut(s) 249, 508, 558, 565, 685
PspPI GGNCC 1 cut(s) 482
PspXI VCTCGAGB 2 cut(s) 149, 378
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 2 cut(s) 34, 710
RsaI GTAC 5 cut(s) 455, 470, 539, 558, 880
RsaNI GTAC 5 cut(s) 454, 469, 538, 557, 879
RseI CAYNNNNRTG 2 cut(s) 627, 924
SaqAI TTAA 2 cut(s) 570, 846
SatI GCNGC 8 cut(s) 32, 271, 384, 533, 682, 699, 711, 944
Sau3AI GATC 6 cut(s) 185, 217, 247, 261, 341, 928
Sau96I GGNCC 1 cut(s) 482
SbfI CCTGCAGG 1 cut(s) 745
SchI GAGTC 2 cut(s) 520, 628
ScrFI CCNGG 8 cut(s) 94, 444, 445, 561, 576, 615, 616, 657
SdaI CCTGCAGG 1 cut(s) 745
SfaNI GCATC 1 cut(s) 710
SfcI CTRYAG 2 cut(s) 511, 741
Sfr274I CTCGAG 2 cut(s) 149, 378
SinI GGWCC 1 cut(s) 482
SlaI CTCGAG 2 cut(s) 149, 378
SmaI CCCGGG 2 cut(s) 445, 616
SmiMI CAYNNNNRTG 2 cut(s) 627, 924
SmlI CTYRAG 2 cut(s) 149, 378
SmoI CTYRAG 2 cut(s) 149, 378
Sse8387I CCTGCAGG 1 cut(s) 745
Sse9I AATT 6 cut(s) 40, 488, 596, 674, 808, 848
SsiI CCGC 6 cut(s) 180, 271, 310, 374, 681, 944
StyD4I CCNGG 8 cut(s) 92, 442, 443, 559, 574, 613, 614, 655
TaaI ACNGT 3 cut(s) 515, 568, 637
TaiI ACGT 2 cut(s) 65, 124
TaqI TCGA 4 cut(s) 129, 150, 264, 379
TaqII GACCGA 1 cut(s) 350
TasI AATT 6 cut(s) 40, 488, 596, 674, 808, 848
TauI GCSGC 3 cut(s) 273, 684, 946
TfiI GAWTC 2 cut(s) 224, 724
Tru1I TTAA 2 cut(s) 570, 846
Tru9I TTAA 2 cut(s) 570, 846
TseFI GTSAC 2 cut(s) 122, 205
TseI GCWGC 5 cut(s) 31, 383, 532, 698, 710
Tsp45I GTSAC 2 cut(s) 122, 205
TspDTI ATGAA 3 cut(s) 317, 576, 791
TspMI CCCGGG 2 cut(s) 443, 614
Van91I CCANNNNNTGG 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 488, 848
XceI RCATGY 1 cut(s) 923
XhoI CTCGAG 2 cut(s) 149, 378
XmaI CCCGGG 2 cut(s) 443, 614
XmnI GAANNNNTTC 1 cut(s) 74
ZraI GACGTC 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.