Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0002270

Physical Location & Seq

Genomic Coordinates Reverse (-)

23808323 .. 23812936

Gene Structure 4614 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1101600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3234 bp

ATGGTGACCGCGACACCACTAACTCCTCCTTCAAACAGCCAGCAAGCCGAAGCCGAAGCCGAGGCTGAGAATCTGAGCGTCCCGGAGTTGTTTTCGGCGCTGAGAAGGGCATCTCAGGCCGATGAATTCGCCCGAATCGAAGAGGCATTAGCAGCGAGAGAAGCGAAGCTGAAGCAAGAAATTGAGCGGCAGAGGCACGAGAAGGCATTGATGGAGGAAAACCACGAGTTCGAGCGGCTCGAGAAGCTCAAAGCAGAGGACGAGCTCAGGCTCAAGCTTTCTGCGGTCAAGGTAGAGGCGGTGAGCGGAGAGGAGCTGCCGGAGAATGACGAGGACAATGAGGTAGTTTCGGAGCGTCCGGGGGAAAAATTGAGGAGGACGAAGAGGAGGAAGGGTTTGAGTGAGAGTGACGGCATGCAGAGAAGAAGAAGCGAGCGTTTGGCTGCAATCGCTACTGATAGTGCTGCAGACAACTCTGCTTCTGATGAAGAAAATGGAGGCCTGACAATTTCAGGTTATAGAGGGGACGAAAGGAAGCCGAAGAAGTGGAAGAACATGGGAAAATTGATGGAAAAGCGGAAGACGGGAAGTGGAGGAAAATATATACAATATCGTTGTACTATGATGTCATTTTTTAATACGATGAAGCGAGTTAAGAAACACTTGAAGGAAGGGCACTTGAAGTTGCTTCAACAAACTCCATTTTGGCCATTAATATCGCCATTTTACAATGGTGTGATTTCCGAAGATCAATGCAGGAAACCAGTGTCTGGTATCCGCAACATCATCAAGTGCTACAACTCCACAACAATGAGTTTTGAGTTTGGTAGCACATCTGCAAGGATGACAACCGAAGATATCGCTGAAATTTTGGGACTTCCTCTAGAAGGTGAAGAAGTACAATTGAAAGGATCAACAAGATACAGATCAGATTTCACCAACAGATATTTCGATGTAAAAGAAGTGTCCAAAAAGATGGTGGATGATGCTTTAGAAGAAGCGATAAAAGGGAAGAGAGAAATCGATGTTGTACGCCTGATTGTACTAGTACTATGCGTTTCCTTCTTATTTAGTGGCACAACCCACTTCACTTCATGGAATATAGTCAAGTATTGTGAAGATTTAGAGAATATTTCAAGATATTCGTGGGCAAAAGCAGTGGCTGACCTTTTACACAAATCATTGCAAGCATCGACCATGAGGTTCGATGGATGTACTGTTCATGGATGTGTGGTTGCCATAATGATTTGGTTGTGTGAGAAAACTAACCTCATACAACCAATCAGTGGAAGGGAGGGACAGAAACCAGCTCTTGTAAAGTGGAGCCTGCAAGAACTGCACATGAAACTGCAAGAAATAGACATTGCTGACGTTGGGCTGAGCTTTAAGAAGGACAAGGGGAAGAAGAAGAAAAGAGGAGAAAACAAAACAACAGGTGAAGAAGCTAAAACTAATGGACGAGAAGATCGAGAACAAGGGGAAGATGTGTTTGAGTTGGAGACCTGCAGTTCGCAGTTTGAGCAGCGTAGTAAGAAATTGAGGAAGGGCAACAACTTGCAAAGAAGAAGTGAGCGTCTGACTGCAAACCGTAGTGCTGCAAACAATAGTAGCAGTGTAAGCTCTCTTCCTGTTAAAGGAAATAAAGATCTCGGAATTTCAGGTTATATAGGGGTCACAAAGAAGCAGATGGAAAATAGGAAAACACAAAAAAGGAAGATGAGAACGTGGACAAGTGCAGAAGGGGGAAAACATGTGCAATACCGCTGTAATATGAAGTCATTTTTTAATACAATGCAGCGAGTTAAGAAACACTTAACTGAAGGGCACTTGGAGTTACTTCGGCAAACTCCATTTTGGCCATTAATATCTGCATTTTACTATGGTGTGATTTCTGAAGATCAATGCAGGAAATCAGACAGTGATGTCCTTAAAATCATCAGATGCTACAACTCAATAACAATGAGTTTTGAGTTTGGTAGCACATCAGCATTGTTGACAACCGAAGACATTGCCAAAATTCTGGGACTCCCACAAGAAGGTAATGAAGTACAATTACAAGGATCAAAGAAGTACGAATCAGTTTTTACCAAAAAATATTTCGAGGAGAAACACGTGTCAAGAAAAATGGTGCACGATGCTTTGGAAGAAGCAATAAAAGGGAAGAGAGAAAAACATGTAGAGGATGTTGTACGCTTGATTGTACTAGAACTATGCGGTACCTTCTTGTTTTGTGACTCAGGTGGGTTAAATTCATGGAGTCTAGTAAAGTATTGCGAAGATTTAGAAAATATTTCAAGATATTCATGGGCAAAAGCAGTGGCTGACCTTCTACACGAGTCGTTGGGAAAACGGACCAAGATGTCGGATGGATATTCTCATCCTGGATGTGTAGTTGTCATAATGCTTTGGTTGTGTGAGAGAACTAACCTTATACGACCAATCAAAGGAAGGGAGGGACAAAGACCAGATCTCGTAAAATGGAACTTGCAGGAACTGCACTCAAAACTGGAACAAATAGACGTCGCTGACATTGGCCTGAGCTTCAAGGACAAGGAGAAGAAGAAGAAAAGAAAATATAATGGGAAAACACACGAGGAAGCTGAAATTGATGGACAAGAAGATGGAGAAGAAGGGGAAGATGTGTTTCGTGATACAGTGGGCTCATCGGAAGACAAACTCCGAGTTCAAAAGACACAATCACAATTCATCCCTGCTCCCACTATTTCGAAAATGGTTCAAAAAGCAAATGCTGAAGTTTCAAGAGCTCAAAGAAAAGTGGACAGGCTAACTGGAAGGTTGGAGAGTAAGAAGGAAAAAACTATAAAACTGAGGAGAAAACTAAAAGAAGAAAAGGAGGAAAAGAGAAAGCTGAAAGAGGACTACGAGTTCGTAATTGCCGAGCAGAAATCATTGTTCGGTTCTCTGAAAGGTTTGGTGAAAAAGCTGGAAGGAACACTCGAAGCCGAAAGGGAAGAGGTGAAAAACCTAAAGAAAGAAAAAACGGAGCTGATCAAGGAAAACCAAGAACTCAAAGATCAGCTACATCAAGGGAGTCCGTGCACCGCAATGCAATTGAGAGAAGACGTACAAATTGTGACAAGCACCGTAAAGAGGGCGTTAGAACCGGAGTTCGGGGACGATCAAGGCGGAAAGAAGAAAAGGAAAGGAGATGAGCAGAATGAAACAAGGGTTCATGTTTTATCTGATGCCACTGCTGCAGGTATTCTTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1985 PMD

Protein Analysis

1078

Amino Acids

122.82

Weight (kDa)

8.98

Isoelectric Point (pI)

42.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMD	PF10536	262 - 448	1.4e-10	Plant mobile domain
DUF1985	PF09331	264 - 393	7.7e-06	Domain of unknown function (DUF1985)
PMD	PF10536	637 - 842	8.6e-12	Plant mobile domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002270)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g04060
malus_domestica	MD06G1101600.v1.1 MD06G1101700.v1.1 MD06G1101900.v1.1 MD14G1121200.v1.1
prunus_persica	Prupe.5G115000_v2.0.a1 Prupe.5G115000_v2.0.a1 Prupe.5G115000_v2.0.a1
pyrus_communis	pycom06g09680
rosa_chinensis	RchiOBHm_Chr5g0001951 RchiOBHm_Chr7g0195461
rosa_laevigata	RLG00000002439 RLG00000004119 RLG00000022620 RLG00000028643 RLG00000028651 RLG00000032833 RLG00000033240
rosa_multiflora	Rmu_sc0000317.1_g000011 Rmu_sc0018163.1_g000004
rosa_roxburghii	Rroxscaffold_2G00094000 Rroxscaffold_3G00260460 Rroxscaffold_5G00357160
rosa_rugosa	Rorug02G0433400 Rorug07G0023400
rosa_samantha	Rh2AG537600 Rh2BG632300 Rh2DG488700 Rh2DG645100 Rh3BG060600 Rh3CG059400 Rh6AG052600 Rh6AG097200 Rh6CG084100 Rh6CG182500 Rh6DG080300 Rh6DG173900 Rh7AG150900 Rh7BG152600 Rh7CG156100 Rh7CG185200 Rh7DG151900
rosa_wichuraiana	Rw7G012880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1922, 2359
AatII	GACGTC	1 cut(s)	2523
Acc36I	ACCTGC	2 cut(s)	1511, 3209
Acc65I	GGTACC	1 cut(s)	2216
AccB1I	GGYRCC	1 cut(s)	2216
AccB7I	CCANNNNNTGG	2 cut(s)	770, 1286
AccBSI	CCGCTC	3 cut(s)	187, 235, 306
AccII	CGCG	1 cut(s)	11
AclWI	GGATC	2 cut(s)	919, 2068
AcoI	YGGCCR	2 cut(s)	707, 1856
AcsI	RAATTY	5 cut(s)	125, 867, 1653, 2016, 2248
AcuI	CTGAAG	4 cut(s)	191, 1839, 1914, 2772
AcvI	CACGTG	1 cut(s)	2113
AcyI	GRCGYC	1 cut(s)	2520
AfiI	CCNNNNNNNGG	8 cut(s)	770, 887, 1286, 1634, 2036, 2444, 3111, 3131
AflIII	ACRYGT	4 cut(s)	1750, 2110, 2112, 2173
AhlI	ACTAGT	1 cut(s)	1045
AjnI	CCWGG	1 cut(s)	2380
AjuI	GAANNNNNNNTTGG	4 cut(s)	932, 964, 2081, 2113
AloI	GAACNNNNNNTCC	4 cut(s)	1203, 1235, 2870, 2902
Alw21I	GWGCWC	4 cut(s)	267, 2133, 2767, 3062
Alw26I	GTCTC	1 cut(s)	1493
Alw44I	GTGCAC	2 cut(s)	2129, 3058
AlwI	GGATC	2 cut(s)	919, 2068
AlwNI	CAGNNNCTG	4 cut(s)	770, 1163, 2321, 2494
Ama87I	CYCGRG	1 cut(s)	239
AoxI	GGCC	5 cut(s)	117, 499, 707, 1856, 2533
ApaLI	GTGCAC	2 cut(s)	2129, 3058
ApeKI	GCWGC	8 cut(s)	152, 316, 443, 464, 1522, 1595, 1795, 3215
ApoI	RAATTY	5 cut(s)	125, 867, 1653, 2016, 2248
ArsI	GACNNNNNNTTYG	4 cut(s)	1492, 1524, 2870, 2902
AseI	ATTAAT	2 cut(s)	713, 1862
Asp718I	GGTACC	1 cut(s)	2216
AspLEI	GCGC	1 cut(s)	100
AspS9I	GGNCC	1 cut(s)	2352
AsuC2I	CCSGG	2 cut(s)	83, 360
AsuHPI	GGTGA	7 cut(s)	16, 313, 902, 928, 1448, 2947, 2989
AsuII	TTCGAA	1 cut(s)	2726
AvaI	CYCGRG	1 cut(s)	239
AvaII	GGWCC	1 cut(s)	2352
BaeGI	GKGCMC	4 cut(s)	678, 1827, 2133, 3062
BalI	TGGCCA	2 cut(s)	709, 1858
BanI	GGYRCC	1 cut(s)	2216
BanII	GRGCYC	3 cut(s)	267, 2663, 2767
BarI	GAAGNNNNNNTAC	4 cut(s)	1608, 1640, 2037, 2069
BauI	CACGAG	4 cut(s)	197, 224, 2333, 2591
BbrPI	CACGTG	1 cut(s)	2113
BbsI	GAAGAC	4 cut(s)	587, 2010, 2676, 3087
Bbv12I	GWGCWC	4 cut(s)	267, 2133, 2767, 3062
BbvI	GCAGC	8 cut(s)	164, 303, 430, 451, 1534, 1582, 1807, 3202
BccI	CCATC	8 cut(s)	205, 562, 970, 1202, 1681, 2360, 2603, 2615
BceAI	ACGGC	1 cut(s)	427
BcgI	CGANNNNNNTGC	2 cut(s)	1991, 2025
BciT130I	CCWGG	1 cut(s)	2382
BciVI	GTATCC	1 cut(s)	785
BclI	TGATCA	1 cut(s)	3009
BcnI	CCSGG	2 cut(s)	83, 360
BcoDI	GTCTC	1 cut(s)	1493
BcuI	ACTAGT	1 cut(s)	1045
BfaI	CTAG	4 cut(s)	884, 1046, 2204, 2261
BfmI	CTRYAG	3 cut(s)	465, 1504, 3216
BfoI	RGCGCY	1 cut(s)	101
BfuAI	ACCTGC	2 cut(s)	1511, 3209
BfuI	GTATCC	1 cut(s)	785
BglII	AGATCT	2 cut(s)	1645, 2467
BlpI	GCTNAGC	1 cut(s)	1379
BmcAI	AGTACT	1 cut(s)	1050
Bme1390I	CCNGG	3 cut(s)	83, 360, 2382
Bme18I	GGWCC	1 cut(s)	2352
BmeT110I	CYCGRG	1 cut(s)	239
BmgT120I	GGNCC	1 cut(s)	2352
BmiI	GGNNCC	2 cut(s)	1325, 2218
BmrFI	CCNGG	3 cut(s)	83, 360, 2382
BmsI	GCATC	6 cut(s)	119, 976, 1199, 1931, 2125, 3196
BpiI	GAAGAC	4 cut(s)	587, 2010, 2676, 3087
Bpu10I	CCTNAGC	2 cut(s)	266, 2537
Bpu1102I	GCTNAGC	1 cut(s)	1379
Bpu14I	TTCGAA	1 cut(s)	2726
BpuEI	CTTGAG	1 cut(s)	257
BpuMI	CCSGG	2 cut(s)	83, 360
Bsa29I	ATCGAT	1 cut(s)	1023
BsaAI	YACGTR	1 cut(s)	2113
BsaBI	GATNNNNATC	1 cut(s)	925
BsaHI	GRCGYC	1 cut(s)	2520
BsaI	GGTCTC	1 cut(s)	1493
BsaJI	CCNNGG	2 cut(s)	60, 359
BsaWI	WCCGGW	1 cut(s)	3124
BsaXI	ACNNNNNCTCC	2 cut(s)	379, 409
Bsc4I	CCNNNNNNNGG	8 cut(s)	770, 887, 1286, 1634, 2036, 2444, 3111, 3131
Bse1I	ACTGG	3 cut(s)	764, 2511, 2794
Bse3DI	GCAATG	4 cut(s)	1181, 1362, 2007, 3072
Bse8I	GATNNNNATC	1 cut(s)	925
BseBI	CCWGG	1 cut(s)	2382
BseCI	ATCGAT	1 cut(s)	1023
BseDI	CCNNGG	2 cut(s)	60, 359
BseGI	GGATG	9 cut(s)	849, 988, 1217, 1232, 2188, 2371, 2377, 2390, 2706
BseJI	GATNNNNATC	1 cut(s)	925
BseLI	CCNNNNNNNGG	8 cut(s)	770, 887, 1286, 1634, 2036, 2444, 3111, 3131
BseMI	GCAATG	4 cut(s)	1181, 1362, 2007, 3072
BseMII	CTCAG	9 cut(s)	57, 65, 92, 128, 280, 1370, 2250, 2528, 2819
BseNI	ACTGG	3 cut(s)	764, 2511, 2794
BseRI	GAGGAG	7 cut(s)	15, 326, 388, 400, 1431, 2117, 2845
BseSI	GKGCMC	4 cut(s)	678, 1827, 2133, 3062
BseXI	GCAGC	8 cut(s)	164, 303, 430, 451, 1534, 1582, 1807, 3202
BsgI	GTGCAG	3 cut(s)	1322, 1755, 2480
Bsh1236I	CGCG	1 cut(s)	11
BshFI	GGCC	5 cut(s)	119, 501, 709, 1858, 2535
BshNI	GGYRCC	1 cut(s)	2216
BshVI	ATCGAT	1 cut(s)	1023
BsiHKAI	GWGCWC	4 cut(s)	267, 2133, 2767, 3062
BsiHKCI	CYCGRG	1 cut(s)	239
BsiSI	CCGG	4 cut(s)	83, 320, 359, 3125
BslFI	GGGAC	7 cut(s)	65, 539, 888, 1311, 2037, 2469, 3149
BslI	CCNNNNNNNGG	8 cut(s)	770, 887, 1286, 1634, 2036, 2444, 3111, 3131
BsmAI	GTCTC	1 cut(s)	1493
BsmFI	GGGAC	7 cut(s)	65, 539, 888, 1311, 2037, 2469, 3149
BsnI	GGCC	5 cut(s)	119, 501, 709, 1858, 2535
Bso31I	GGTCTC	1 cut(s)	1493
BsoBI	CYCGRG	1 cut(s)	239
Bsp119I	TTCGAA	1 cut(s)	2726
Bsp1286I	GDGCHC	7 cut(s)	267, 678, 1827, 2133, 2663, 2767, 3062
Bsp1720I	GCTNAGC	1 cut(s)	1379
BspANI	GGCC	5 cut(s)	119, 501, 709, 1858, 2535
BspCNI	CTCAG	9 cut(s)	58, 66, 93, 127, 279, 1371, 2249, 2529, 2820
BspDI	ATCGAT	1 cut(s)	1023
BspFNI	CGCG	1 cut(s)	11
BspLI	GGNNCC	2 cut(s)	1325, 2218
BspMAI	CTGCAG	3 cut(s)	469, 1508, 3220
BspMI	ACCTGC	2 cut(s)	1511, 3209
BspPI	GGATC	2 cut(s)	919, 2068
BspT104I	TTCGAA	1 cut(s)	2726
BspT107I	GGYRCC	1 cut(s)	2216
BspTNI	GGTCTC	1 cut(s)	1493
BsrBI	CCGCTC	3 cut(s)	187, 235, 306
BsrDI	GCAATG	4 cut(s)	1181, 1362, 2007, 3072
BsrI	ACTGG	3 cut(s)	764, 2511, 2794
BssECI	CCNNGG	2 cut(s)	60, 359
BssNI	GRCGYC	1 cut(s)	2520
BssSI	CACGAG	4 cut(s)	197, 224, 2333, 2591
Bst2BI	CACGAG	4 cut(s)	197, 224, 2333, 2591
Bst2UI	CCWGG	1 cut(s)	2382
Bst4CI	ACNGT	5 cut(s)	1219, 1589, 1919, 2656, 3106
Bst6I	CTCTTC	6 cut(s)	135, 377, 1007, 1629, 2156, 2967
BstACI	GRCGYC	1 cut(s)	2520
BstAPI	GCANNNNNTGC	2 cut(s)	1336, 2494
BstBAI	YACGTR	1 cut(s)	2113
BstBI	TTCGAA	1 cut(s)	2726
BstC8I	GCNNGC	6 cut(s)	41, 45, 416, 434, 1188, 1328
BstDEI	CTNAG	9 cut(s)	66, 74, 101, 114, 266, 1379, 2236, 2537, 2828
BstEII	GGTNACC	1 cut(s)	4
BstENI	CCTNNNNNAGG	2 cut(s)	885, 1632
BstF5I	GGATG	9 cut(s)	849, 988, 1217, 1232, 2188, 2371, 2377, 2390, 2706
BstFNI	CGCG	1 cut(s)	11
BstH2I	RGCGCY	1 cut(s)	101
BstHHI	GCGC	1 cut(s)	100
BstMAI	GTCTC	1 cut(s)	1493
BstNI	CCWGG	1 cut(s)	2382
BstNSI	RCATGY	3 cut(s)	418, 1754, 2177
BstPI	GGTNACC	1 cut(s)	4
BstSCI	CCNGG	3 cut(s)	81, 358, 2380
BstSFI	CTRYAG	3 cut(s)	465, 1504, 3216
BstSLI	GKGCMC	4 cut(s)	678, 1827, 2133, 3062
BstUI	CGCG	1 cut(s)	11
BstV1I	GCAGC	8 cut(s)	164, 303, 430, 451, 1534, 1582, 1807, 3202
BstV2I	GAAGAC	4 cut(s)	587, 2010, 2676, 3087
BstX2I	RGATCY	2 cut(s)	1645, 2467
BstXI	CCANNNNNNTGG	2 cut(s)	976, 2020
BstYI	RGATCY	2 cut(s)	1645, 2467
Bsu15I	ATCGAT	1 cut(s)	1023
BsuI	GTATCC	1 cut(s)	785
BsuRI	GGCC	5 cut(s)	119, 501, 709, 1858, 2535
BsuTUI	ATCGAT	1 cut(s)	1023
BtsCI	GGATG	9 cut(s)	849, 988, 1217, 1232, 2188, 2371, 2377, 2390, 2706
BtsI	GCAGTG	4 cut(s)	1164, 1618, 2322, 3210
BtsIMutI	CAGTG	8 cut(s)	771, 1164, 1291, 1618, 1924, 2322, 2661, 3210
BveI	ACCTGC	2 cut(s)	1511, 3209
Cac8I	GCNNGC	6 cut(s)	41, 45, 416, 434, 1188, 1328
CaiI	CAGNNNCTG	4 cut(s)	770, 1163, 2321, 2494
CfoI	GCGC	1 cut(s)	100
Cfr13I	GGNCC	1 cut(s)	2352
ClaI	ATCGAT	1 cut(s)	1023
CseI	GACGC	3 cut(s)	67, 344, 1562
CspCI	CAANNNNNGTGG	4 cut(s)	1140, 1175, 2298, 2333
DdeI	CTNAG	9 cut(s)	66, 74, 101, 114, 266, 1379, 2236, 2537, 2828
DrdI	GACNNNNNNGTC	2 cut(s)	1922, 2359
DseDI	GACNNNNNNGTC	2 cut(s)	1922, 2359
EaeI	YGGCCR	2 cut(s)	707, 1856
Eam1104I	CTCTTC	6 cut(s)	135, 377, 1007, 1629, 2156, 2967
EarI	CTCTTC	6 cut(s)	135, 377, 1007, 1629, 2156, 2967
EciI	GGCGGA	1 cut(s)	3162
Ecl136II	GAGCTC	2 cut(s)	265, 2765
Eco147I	AGGCCT	1 cut(s)	501
Eco24I	GRGCYC	3 cut(s)	267, 2663, 2767
Eco31I	GGTCTC	1 cut(s)	1493
Eco32I	GATATC	1 cut(s)	859
Eco47I	GGWCC	1 cut(s)	2352
Eco53kI	GAGCTC	2 cut(s)	265, 2765
Eco57I	CTGAAG	4 cut(s)	191, 1839, 1914, 2772
Eco72I	CACGTG	1 cut(s)	2113
Eco88I	CYCGRG	1 cut(s)	239
Eco91I	GGTNACC	1 cut(s)	4
EcoICRI	GAGCTC	2 cut(s)	265, 2765
EcoNI	CCTNNNNNAGG	2 cut(s)	885, 1632
EcoO65I	GGTNACC	1 cut(s)	4
EcoRI	GAATTC	1 cut(s)	125
EcoRII	CCWGG	1 cut(s)	2380
EcoRV	GATATC	1 cut(s)	859
EcoT38I	GRGCYC	3 cut(s)	267, 2663, 2767
FalI	AAGNNNNNCTT	4 cut(s)	647, 679, 1796, 1828
FaqI	GGGAC	7 cut(s)	65, 539, 888, 1311, 2037, 2469, 3149
FbaI	TGATCA	1 cut(s)	3009
FokI	GGATG	9 cut(s)	856, 995, 1224, 1239, 2195, 2364, 2378, 2397, 2693
FriOI	GRGCYC	3 cut(s)	267, 2663, 2767
FspBI	CTAG	4 cut(s)	884, 1046, 2204, 2261
GlaI	GCGC	1 cut(s)	99
HaeII	RGCGCY	1 cut(s)	101
HaeIII	GGCC	5 cut(s)	119, 501, 709, 1858, 2535
HapII	CCGG	4 cut(s)	83, 320, 359, 3125
HgaI	GACGC	3 cut(s)	67, 344, 1562
HhaI	GCGC	1 cut(s)	100
Hin1I	GRCGYC	1 cut(s)	2520
Hin6I	GCGC	1 cut(s)	98
HinP1I	GCGC	1 cut(s)	98
HincII	GTYRAC	1 cut(s)	1995
HindII	GTYRAC	1 cut(s)	1995
HindIII	AAGCTT	1 cut(s)	275
HinfI	GANTC	8 cut(s)	70, 135, 2025, 2075, 2234, 2257, 2336, 3052
HpaII	CCGG	4 cut(s)	83, 320, 359, 3125
HphI	GGTGA	7 cut(s)	16, 313, 902, 928, 1448, 2947, 2989
Hpy166II	GTNNAC	5 cut(s)	1728, 1995, 2131, 2779, 3060
Hpy188III	TCNNGA	8 cut(s)	241, 884, 1137, 1469, 2118, 2295, 2648, 2760
Hpy8I	GTNNAC	5 cut(s)	1728, 1995, 2131, 2779, 3060
Hpy99I	CGWCG	1 cut(s)	2525
HpyCH4III	ACNGT	5 cut(s)	1219, 1589, 1919, 2656, 3106
HpyCH4IV	ACGT	5 cut(s)	1371, 1724, 2112, 2520, 3084
HpyF3I	CTNAG	9 cut(s)	66, 74, 101, 114, 266, 1379, 2236, 2537, 2828
HpySE526I	ACGT	5 cut(s)	1371, 1724, 2112, 2520, 3084
Hsp92I	GRCGYC	1 cut(s)	2520
HspAI	GCGC	1 cut(s)	98
KpnI	GGTACC	1 cut(s)	2220
Ksp22I	TGATCA	1 cut(s)	3009
LmnI	GCTCC	5 cut(s)	313, 352, 1323, 2719, 3004
Lsp1109I	GCAGC	8 cut(s)	164, 303, 430, 451, 1534, 1582, 1807, 3202
LweI	GCATC	6 cut(s)	119, 976, 1199, 1931, 2125, 3196
MaeI	CTAG	4 cut(s)	884, 1046, 2204, 2261
MaeII	ACGT	5 cut(s)	1371, 1724, 2112, 2520, 3084
MaeIII	GTNAC	6 cut(s)	4, 407, 1672, 1833, 2231, 3094
MbiI	CCGCTC	3 cut(s)	187, 235, 306
MfeI	CAATTG	2 cut(s)	902, 3071
MflI	RGATCY	2 cut(s)	1645, 2467
MhlI	GDGCHC	7 cut(s)	267, 678, 1827, 2133, 2663, 2767, 3062
MlsI	TGGCCA	2 cut(s)	709, 1858
MluNI	TGGCCA	2 cut(s)	709, 1858
MlyI	GAGTC	5 cut(s)	2019, 2228, 2266, 2345, 3061
MmeI	TCCRAC	3 cut(s)	1476, 2343, 2778
Mox20I	TGGCCA	2 cut(s)	709, 1858
MscI	TGGCCA	2 cut(s)	709, 1858
MslI	CAYNNNNRTG	3 cut(s)	809, 1227, 3065
Msp20I	TGGCCA	2 cut(s)	709, 1858
MspA1I	CMGCKG	1 cut(s)	1764
MspI	CCGG	4 cut(s)	83, 320, 359, 3125
MspR9I	CCNGG	3 cut(s)	83, 360, 2382
MunI	CAATTG	2 cut(s)	902, 3071
MvaI	CCWGG	1 cut(s)	2382
MvnI	CGCG	1 cut(s)	11
NciI	CCSGG	2 cut(s)	83, 360
NlaIV	GGNNCC	2 cut(s)	1325, 2218
NmeAIII	GCCGAG	2 cut(s)	85, 2923
NmuCI	GTSAC	5 cut(s)	4, 407, 1672, 2231, 3094
NspI	RCATGY	3 cut(s)	418, 1754, 2177
NspV	TTCGAA	1 cut(s)	2726
PaeI	GCATGC	1 cut(s)	418
PaeR7I	CTCGAG	1 cut(s)	239
PceI	AGGCCT	1 cut(s)	501
PciI	ACATGT	2 cut(s)	1750, 2173
PcsI	WCGNNNNNNNCGW	4 cut(s)	135, 237, 1465, 2895
PfeI	GAWTC	3 cut(s)	70, 135, 2075
PflMI	CCANNNNNTGG	2 cut(s)	770, 1286
PfoI	TCCNGGA	2 cut(s)	81, 2380
PleI	GAGTC	5 cut(s)	2019, 2228, 2265, 2344, 3060
PmaCI	CACGTG	1 cut(s)	2113
PmlI	CACGTG	1 cut(s)	2113
PpsI	GAGTC	5 cut(s)	2019, 2228, 2265, 2344, 3060
Ppu21I	YACGTR	1 cut(s)	2113
PscI	ACATGT	2 cut(s)	1750, 2173
PshBI	ATTAAT	2 cut(s)	713, 1862
Psp124BI	GAGCTC	2 cut(s)	267, 2767
Psp6I	CCWGG	1 cut(s)	2380
PspCI	CACGTG	1 cut(s)	2113
PspEI	GGTNACC	1 cut(s)	4
PspGI	CCWGG	1 cut(s)	2380
PspN4I	GGNNCC	2 cut(s)	1325, 2218
PspPI	GGNCC	1 cut(s)	2352
PstI	CTGCAG	3 cut(s)	469, 1508, 3220
PstNI	CAGNNNCTG	4 cut(s)	770, 1163, 2321, 2494
PsuI	RGATCY	2 cut(s)	1645, 2467
RseI	CAYNNNNRTG	3 cut(s)	809, 1227, 3065
SacI	GAGCTC	2 cut(s)	267, 2767
Sau96I	GGNCC	1 cut(s)	2352
ScaI	AGTACT	1 cut(s)	1050
SchI	GAGTC	5 cut(s)	2019, 2228, 2266, 2345, 3061
ScrFI	CCNGG	3 cut(s)	83, 360, 2382
SduI	GDGCHC	7 cut(s)	267, 678, 1827, 2133, 2663, 2767, 3062
SfaNI	GCATC	6 cut(s)	119, 976, 1199, 1931, 2125, 3196
SfcI	CTRYAG	3 cut(s)	465, 1504, 3216
Sfr274I	CTCGAG	1 cut(s)	239
SfuI	TTCGAA	1 cut(s)	2726
SinI	GGWCC	1 cut(s)	2352
SlaI	CTCGAG	1 cut(s)	239
SmiMI	CAYNNNNRTG	3 cut(s)	809, 1227, 3065
SmlI	CTYRAG	2 cut(s)	239, 272
SmoI	CTYRAG	2 cut(s)	239, 272
SpeI	ACTAGT	1 cut(s)	1045
SphI	GCATGC	1 cut(s)	418
SseBI	AGGCCT	1 cut(s)	501
SspI	AATATT	3 cut(s)	1132, 2096, 2290
SspMI	CTAG	4 cut(s)	884, 1046, 2204, 2261
SstI	GAGCTC	2 cut(s)	267, 2767
StuI	AGGCCT	1 cut(s)	501
StyD4I	CCNGG	3 cut(s)	81, 358, 2380
TaaI	ACNGT	5 cut(s)	1219, 1589, 1919, 2656, 3106
TaiI	ACGT	5 cut(s)	1374, 1727, 2115, 2523, 3087
TatI	WGTACW	7 cut(s)	617, 898, 1042, 1048, 1214, 2047, 2200
TauI	GCSGC	2 cut(s)	190, 238
TfiI	GAWTC	3 cut(s)	70, 135, 2075
TscAI	CASTG	8 cut(s)	771, 1164, 1291, 1618, 1924, 2322, 2661, 3217
TseFI	GTSAC	5 cut(s)	4, 407, 1672, 2231, 3094
TseI	GCWGC	8 cut(s)	152, 316, 443, 464, 1522, 1595, 1795, 3215
Tsp45I	GTSAC	5 cut(s)	4, 407, 1672, 2231, 3094
TspGWI	ACGGA	3 cut(s)	2365, 3017, 3045
TspRI	CASTG	8 cut(s)	771, 1164, 1291, 1618, 1924, 2322, 2661, 3217
Van91I	CCANNNNNTGG	2 cut(s)	770, 1286
VneI	GTGCAC	2 cut(s)	2129, 3058
VpaK11BI	GGWCC	1 cut(s)	2352
VspI	ATTAAT	2 cut(s)	713, 1862
XagI	CCTNNNNNAGG	2 cut(s)	885, 1632
XapI	RAATTY	5 cut(s)	125, 867, 1653, 2016, 2248
XbaI	TCTAGA	1 cut(s)	883
XceI	RCATGY	3 cut(s)	418, 1754, 2177
XcmI	CCANNNNNNNNNTGG	1 cut(s)	976
XhoI	CTCGAG	1 cut(s)	239
XspI	CTAG	4 cut(s)	884, 1046, 2204, 2261
ZraI	GACGTC	1 cut(s)	2521
ZrmI	AGTACT	1 cut(s)	1050

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.