MD06G1114600.v1.1

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005386
Physical Location & Seq
Forward (+)
25402702 .. 25405249
2548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1114600.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGCGGAATCCATCATTACCTCAGAAAAACCCTCCACCTCGTCCCCTCTAGAACCTCCTTCCCGTATCGTCTGCCACGTCTGCCAGAAGCAATTTTCTCAGTACACATGCCCTCGATGCAACTCACGCTACTGTTCCCTGCACTGCTACAAGTCGCATAATCTCCGGTGTACCGAATCGTTTATGAAGGAGAATGTGGTGGGGGAGCTGGGGCAATTGGGGCCCGATGACGAAACTAAACAGAAAATGCTGGACATACTGAAACGATTTCACTCTGAAGAAGAAGAAACTGATGACATGGATGATGATGATAATGCTGGTTCTGCACTGTCTGAGGAGACTATTCAAAAAGTGCTGTCTGGTGGTGAAGTTAGTCTGGATGATCTATCTACAGAAGAGAAGAAACTATTCCAAAGAGCTGTCGTTTCTGGGGAACTGAGCAAGATGATTAAGCCTTGGGATCCGTGGTGGTTGAAGCCTTCTGCCAGAACGATATCATTTAGCAAGGAAGGATCCCAACTTATCCAACCTCTTGTCAAAGACGATGAATCAATGTCACCTGAGGATAATTCAGAGAGCAATCAAGCGAGTGAGATTCCACCCGGGCCTGACACCCCACTGCCACCAGTCAGCAAGCTGAGTTCCACAAAGCCATCACCGTCCTTAGCTGTTCATCTGGTTGACATCATATACAGCTACTGTTTCACACTCCGTCTTTACAATGGAGATTGGAAATCGGATGCCATAGGATCATCCGTGGCAGTGCTTTCTGTCTCCAATGTACTGGGCCAGGGTGGGCAACCGGAGACTGTCCTGGAAGTGCTGTCTTATTGCTTGGAGAAGACCTGCTCCCCAGACTTCAAACATACAGGCGGCTTGCAATTTGGACTCGGTCTCATAGATGATGTAACAAGCCTATTAATGCTAGGAACACCCGCTTTGTTGTGCTTGCTCTGTGATCTGCAGAGGATGGTTCAGGCTGGGGAGAGGGATCTGAAGTCAGAGAAACCACGAAAGTTGTCACGGAGAACAGAAATTAGGAACAAGCTTAAGTTGGCCGAACGGAAAATTTATTTCATCATGTGTTGGGTGCACGAGCAAACCGGGGAAGTTTGGACTCCCTTAGCAGCCATTGTAACGGCCGAAAAGAGTTCAGCTTTGAGTTATCAATCGGCTTCGAGGCCCGGGAAAGCGGAGAAGAAAGCCGAACCAAAGAGCAAGGTTTTGATTCAGGAGATTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHQ1 zf-HIT

Protein Analysis

415

Amino Acids

46.01

Weight (kDa)

5.41

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 22 - 51 2e-10 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 854
AciI CCGC 4 cut(s) 5, 873, 936, 1193
AclWI GGATC 6 cut(s) 455, 468, 507, 520, 757, 999
AcoI YGGCCR 2 cut(s) 1056, 1140
AcsI RAATTY 1 cut(s) 1068
AcuI CTGAAG 2 cut(s) 297, 1016
AfaI GTAC 3 cut(s) 104, 172, 783
AflII CTTAAG 1 cut(s) 1049
AgsI TTSAA 3 cut(s) 347, 475, 862
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 2 cut(s) 789, 813
AluBI AGCT 7 cut(s) 208, 419, 637, 668, 696, 1048, 1157
AluI AGCT 7 cut(s) 208, 419, 637, 668, 696, 1048, 1157
Alw21I GWGCWC 1 cut(s) 1095
Alw26I GTCTC 4 cut(s) 332, 778, 800, 899
Alw44I GTGCAC 1 cut(s) 1091
AlwI GGATC 6 cut(s) 455, 468, 507, 520, 757, 999
AlwNI CAGNNNCTG 1 cut(s) 699
Ama87I CYCGRG 2 cut(s) 602, 1184
AoxI GGCC 6 cut(s) 221, 605, 787, 1056, 1140, 1181
ApaI GGGCCC 1 cut(s) 225
ApaLI GTGCAC 1 cut(s) 1091
ApeKI GCWGC 1 cut(s) 1127
ApoI RAATTY 1 cut(s) 1068
AseI ATTAAT 1 cut(s) 920
Asp700I GAANNNNTTC 1 cut(s) 407
AspS9I GGNCC 5 cut(s) 221, 222, 605, 787, 1182
AsuC2I CCSGG 5 cut(s) 603, 604, 1105, 1185, 1186
AsuHPI GGTGA 3 cut(s) 377, 549, 648
AvaI CYCGRG 2 cut(s) 602, 1184
AxyI CCTNAGG 1 cut(s) 561
BaeGI GKGCMC 2 cut(s) 225, 1095
BamHI GGATCC 2 cut(s) 460, 512
BanII GRGCYC 1 cut(s) 225
BauI CACGAG 1 cut(s) 1094
BbsI GAAGAC 1 cut(s) 848
Bbv12I GWGCWC 1 cut(s) 1095
BbvI GCAGC 1 cut(s) 1139
BccI CCATC 3 cut(s) 20, 661, 964
BceAI ACGGC 1 cut(s) 1155
BciT130I CCWGG 2 cut(s) 791, 815
BcnI CCSGG 5 cut(s) 603, 604, 1105, 1185, 1186
BcoDI GTCTC 4 cut(s) 332, 778, 800, 899
BfaI CTAG 2 cut(s) 50, 926
BfmI CTRYAG 2 cut(s) 390, 962
BfrI CTTAAG 1 cut(s) 1049
BfuAI ACCTGC 1 cut(s) 854
BisI GCNGC 2 cut(s) 874, 1128
BlsI GCNGC 2 cut(s) 875, 1129
Bme1390I CCNGG 7 cut(s) 603, 604, 791, 815, 1105, 1185, 1186
BmeT110I CYCGRG 2 cut(s) 602, 1184
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 5 cut(s) 221, 222, 605, 787, 1182
BmiI GGNNCC 4 cut(s) 222, 223, 462, 514
BmrFI CCNGG 7 cut(s) 603, 604, 791, 815, 1105, 1185, 1186
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 2 cut(s) 107, 730
BmuI ACTGGG 1 cut(s) 794
BpiI GAAGAC 1 cut(s) 848
Bpu10I CCTNAGC 2 cut(s) 664, 1123
BpuMI CCSGG 5 cut(s) 603, 604, 1105, 1185, 1186
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 7 cut(s) 455, 464, 602, 756, 790, 1104, 1184
BsaWI WCCGGW 2 cut(s) 165, 802
Bse1I ACTGG 2 cut(s) 626, 789
Bse21I CCTNAGG 1 cut(s) 561
BseBI CCWGG 2 cut(s) 791, 815
BseDI CCNNGG 7 cut(s) 455, 464, 602, 756, 790, 1104, 1184
BseGI GGATG 5 cut(s) 307, 385, 745, 752, 975
BseMII CTCAG 6 cut(s) 36, 113, 324, 428, 552, 629
BseNI ACTGG 2 cut(s) 626, 789
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 2 cut(s) 225, 1095
BseX3I CGGCCG 1 cut(s) 1140
BseXI GCAGC 1 cut(s) 1139
BseYI CCCAGC 2 cut(s) 208, 980
BsgI GTGCAG 2 cut(s) 125, 309
Bsh1285I CGRYCG 1 cut(s) 1143
BshFI GGCC 6 cut(s) 223, 607, 789, 1058, 1142, 1183
BsiEI CGRYCG 1 cut(s) 1143
BsiHKAI GWGCWC 1 cut(s) 1095
BsiHKCI CYCGRG 2 cut(s) 602, 1184
BsiSI CCGG 5 cut(s) 166, 603, 803, 1104, 1185
BslFI GGGAC 1 cut(s) 28
BsmAI GTCTC 4 cut(s) 332, 778, 800, 899
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 6 cut(s) 223, 607, 789, 1058, 1142, 1183
Bso31I GGTCTC 1 cut(s) 899
BsoBI CYCGRG 2 cut(s) 602, 1184
Bsp120I GGGCCC 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 225, 1095
Bsp143I GATC 6 cut(s) 382, 460, 512, 749, 958, 991
BspACI CCGC 4 cut(s) 5, 873, 936, 1193
BspANI GGCC 6 cut(s) 223, 607, 789, 1058, 1142, 1183
BspCNI CTCAG 6 cut(s) 35, 112, 325, 429, 553, 630
BspLI GGNNCC 4 cut(s) 222, 223, 462, 514
BspMAI CTGCAG 1 cut(s) 966
BspMI ACCTGC 1 cut(s) 854
BspPI GGATC 6 cut(s) 455, 468, 507, 520, 757, 999
BspTI CTTAAG 1 cut(s) 1049
BspTNI GGTCTC 1 cut(s) 899
BsrI ACTGG 2 cut(s) 626, 789
BssECI CCNNGG 7 cut(s) 455, 464, 602, 756, 790, 1104, 1184
BssMI GATC 6 cut(s) 382, 460, 512, 749, 958, 991
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 1 cut(s) 455
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 2 cut(s) 791, 815
Bst4CI ACNGT 5 cut(s) 134, 330, 660, 701, 811
Bst6I CTCTTC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 1049
BstC8I GCNNGC 3 cut(s) 635, 878, 950
BstDEI CTNAG 8 cut(s) 22, 99, 333, 437, 561, 638, 664, 1123
BstDSI CCRYGG 2 cut(s) 464, 756
BstF5I GGATG 5 cut(s) 307, 385, 745, 752, 975
BstKTI GATC 6 cut(s) 385, 463, 515, 752, 961, 994
BstMAI GTCTC 4 cut(s) 332, 778, 800, 899
BstMBI GATC 6 cut(s) 382, 460, 512, 749, 958, 991
BstMCI CGRYCG 1 cut(s) 1143
BstMWI GCNNNNNNNGC 5 cut(s) 81, 117, 126, 220, 323
BstNI CCWGG 2 cut(s) 791, 815
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 7 cut(s) 601, 602, 789, 813, 1103, 1183, 1184
BstSFI CTRYAG 2 cut(s) 390, 962
BstSLI GKGCMC 2 cut(s) 225, 1095
BstV1I GCAGC 1 cut(s) 1139
BstV2I GAAGAC 1 cut(s) 848
BstX2I RGATCY 3 cut(s) 460, 512, 991
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 3 cut(s) 460, 512, 991
BstZI CGGCCG 1 cut(s) 1140
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 6 cut(s) 223, 607, 789, 1058, 1142, 1183
BtgI CCRYGG 2 cut(s) 464, 756
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 5 cut(s) 307, 385, 745, 752, 975
BtsI GCAGTG 3 cut(s) 142, 617, 768
BtsIMutI CAGTG 4 cut(s) 142, 326, 617, 768
BveI ACCTGC 1 cut(s) 854
Cac8I GCNNGC 3 cut(s) 635, 878, 950
CaiI CAGNNNCTG 1 cut(s) 699
Cfr13I GGNCC 5 cut(s) 221, 222, 605, 787, 1182
Cfr9I CCCGGG 2 cut(s) 602, 1184
Csp6I GTAC 3 cut(s) 103, 171, 782
CspCI CAANNNNNGTGG 2 cut(s) 999, 1034
CviAII CATG 3 cut(s) 108, 298, 1081
CviQI GTAC 3 cut(s) 103, 171, 782
DdeI CTNAG 8 cut(s) 22, 99, 333, 437, 561, 638, 664, 1123
DpnI GATC 6 cut(s) 384, 462, 514, 751, 960, 993
DpnII GATC 6 cut(s) 382, 460, 512, 749, 958, 991
EaeI YGGCCR 2 cut(s) 1056, 1140
EagI CGGCCG 1 cut(s) 1140
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 1140
Eco130I CCWWGG 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 899
Eco32I GATATC 1 cut(s) 495
Eco52I CGGCCG 1 cut(s) 1140
Eco57I CTGAAG 2 cut(s) 297, 1016
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 2 cut(s) 602, 1184
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 789, 813
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 3 cut(s) 111, 301, 1084
FaqI GGGAC 1 cut(s) 28
FatI CATG 3 cut(s) 107, 297, 1080
FauI CCCGC 1 cut(s) 943
Fnu4HI GCNGC 2 cut(s) 874, 1128
FokI GGATG 5 cut(s) 314, 392, 739, 752, 982
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 2 cut(s) 874, 1128
FspBI CTAG 2 cut(s) 50, 926
GluI GCNGC 2 cut(s) 874, 1128
GsaI CCCAGC 2 cut(s) 212, 984
HaeIII GGCC 6 cut(s) 223, 607, 789, 1058, 1142, 1183
HapII CCGG 5 cut(s) 166, 603, 803, 1104, 1185
Hin1II CATG 3 cut(s) 111, 301, 1084
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HindIII AAGCTT 1 cut(s) 1046
HinfI GANTC 7 cut(s) 8, 176, 548, 595, 888, 1117, 1228
HpaII CCGG 5 cut(s) 166, 603, 803, 1104, 1185
HphI GGTGA 3 cut(s) 377, 549, 648
Hpy166II GTNNAC 4 cut(s) 105, 171, 682, 1093
Hpy188I TCNGA 7 cut(s) 25, 277, 334, 574, 739, 996, 1003
Hpy188III TCNNGA 3 cut(s) 50, 377, 1232
Hpy8I GTNNAC 4 cut(s) 105, 171, 682, 1093
HpyAV CCTTC 4 cut(s) 69, 181, 489, 503
HpyCH4III ACNGT 5 cut(s) 134, 330, 660, 701, 811
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 6 cut(s) 120, 142, 326, 880, 964, 1093
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 117, 126, 220, 323
HpyF3I CTNAG 8 cut(s) 22, 99, 333, 437, 561, 638, 664, 1123
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 3 cut(s) 111, 301, 1084
Kzo9I GATC 6 cut(s) 382, 460, 512, 749, 958, 991
LmnI GCTCC 2 cut(s) 205, 854
Lsp1109I GCAGC 1 cut(s) 1139
LweI GCATC 2 cut(s) 107, 730
MaeI CTAG 2 cut(s) 50, 926
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 4 cut(s) 555, 907, 1020, 1135
MalI GATC 6 cut(s) 384, 462, 514, 751, 960, 993
MboI GATC 6 cut(s) 382, 460, 512, 749, 958, 991
MboII GAAGA 7 cut(s) 290, 293, 296, 407, 412, 853, 1210
MfeI CAATTG 1 cut(s) 215
MflI RGATCY 3 cut(s) 460, 512, 991
MhlI GDGCHC 2 cut(s) 225, 1095
MluCI AATT 6 cut(s) 92, 215, 568, 881, 1035, 1068
MlyI GAGTC 2 cut(s) 882, 1111
MmeI TCCRAC 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 3 cut(s) 450, 920, 1050
MspCI CTTAAG 1 cut(s) 1049
MspI CCGG 5 cut(s) 166, 603, 803, 1104, 1185
MspR9I CCNGG 7 cut(s) 603, 604, 791, 815, 1105, 1185, 1186
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 2 cut(s) 791, 815
MwoI GCNNNNNNNGC 5 cut(s) 81, 117, 126, 220, 323
NciI CCSGG 5 cut(s) 603, 604, 1105, 1185, 1186
NdeII GATC 6 cut(s) 382, 460, 512, 749, 958, 991
NlaIII CATG 3 cut(s) 111, 301, 1084
NlaIV GGNNCC 4 cut(s) 222, 223, 462, 514
NmuCI GTSAC 2 cut(s) 555, 1020
NspI RCATGY 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 5 cut(s) 8, 176, 548, 595, 1228
PflFI GACNNNGTC 1 cut(s) 891
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 2 cut(s) 875, 1129
PleI GAGTC 2 cut(s) 882, 1111
PpsI GAGTC 2 cut(s) 882, 1111
PshBI ATTAAT 1 cut(s) 920
Psp6I CCWGG 2 cut(s) 789, 813
PspFI CCCAGC 2 cut(s) 208, 980
PspGI CCWGG 2 cut(s) 789, 813
PspN4I GGNNCC 4 cut(s) 222, 223, 462, 514
PspOMI GGGCCC 1 cut(s) 221
PspPI GGNCC 5 cut(s) 221, 222, 605, 787, 1182
PstI CTGCAG 1 cut(s) 966
PstNI CAGNNNCTG 1 cut(s) 699
PsuI RGATCY 3 cut(s) 460, 512, 991
PsyI GACNNNGTC 1 cut(s) 891
RsaI GTAC 3 cut(s) 104, 172, 783
RsaNI GTAC 3 cut(s) 103, 171, 782
SaqAI TTAA 3 cut(s) 450, 920, 1050
SatI GCNGC 2 cut(s) 874, 1128
Sau3AI GATC 6 cut(s) 382, 460, 512, 749, 958, 991
Sau96I GGNCC 5 cut(s) 221, 222, 605, 787, 1182
SchI GAGTC 2 cut(s) 882, 1111
ScrFI CCNGG 7 cut(s) 603, 604, 791, 815, 1105, 1185, 1186
SduI GDGCHC 2 cut(s) 225, 1095
SfaNI GCATC 2 cut(s) 107, 730
SfcI CTRYAG 2 cut(s) 390, 962
SmaI CCCGGG 2 cut(s) 604, 1186
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
Sse9I AATT 6 cut(s) 92, 215, 568, 881, 1035, 1068
SsiI CCGC 4 cut(s) 5, 873, 936, 1193
SspMI CTAG 2 cut(s) 50, 926
StyD4I CCNGG 7 cut(s) 601, 602, 789, 813, 1103, 1183, 1184
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 5 cut(s) 134, 330, 660, 701, 811
TaiI ACGT 1 cut(s) 81
TaqI TCGA 2 cut(s) 115, 1178
TaqII GACCGA 1 cut(s) 881
TasI AATT 6 cut(s) 92, 215, 568, 881, 1035, 1068
TatI WGTACW 2 cut(s) 102, 781
TauI GCSGC 1 cut(s) 876
TfiI GAWTC 5 cut(s) 8, 176, 548, 595, 1228
Tru1I TTAA 3 cut(s) 450, 920, 1050
Tru9I TTAA 3 cut(s) 450, 920, 1050
TscAI CASTG 4 cut(s) 149, 333, 624, 768
TseFI GTSAC 2 cut(s) 555, 1020
TseI GCWGC 1 cut(s) 1127
Tsp45I GTSAC 2 cut(s) 555, 1020
TspDTI ATGAA 4 cut(s) 200, 561, 662, 1066
TspGWI ACGGA 5 cut(s) 453, 701, 745, 1039, 1078
TspMI CCCGGG 2 cut(s) 602, 1184
TspRI CASTG 4 cut(s) 149, 333, 624, 768
Tth111I GACNNNGTC 1 cut(s) 891
Vha464I CTTAAG 1 cut(s) 1049
VneI GTGCAC 1 cut(s) 1091
VspI ATTAAT 1 cut(s) 920
XapI RAATTY 1 cut(s) 1068
XbaI TCTAGA 1 cut(s) 49
XceI RCATGY 1 cut(s) 111
XmaI CCCGGG 2 cut(s) 602, 1184
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 2 cut(s) 50, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.