Rroxscaffold_3G00269860

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005386
Physical Location & Seq
Forward (+)
62587642 .. 62588429
788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269860.1

Sequence Viewer

Length: 600 bp
ATGGCGGACTCCATAATCACCTCTGAGGAACCCTCCACCTCGTCCCCTATCGACCCTCCTTCCCGCATCGTCTGCCATGTTTGCCTGAAGCAGTTTTCACAGTACACGTGTCCTCGATGCAACTCACGCTACTGTTCTCTTCACTGCTACAAGTCTCATAATATTCGATGTACCGAGTCGTTTATGCGAGATAATGTGCTTGGGGAGCTAGGGCAATTGGGGCCGAATGACGAAACGAAGCAGAAGATGATGGACATACTGAAAAGGTTTCACTCTGAAGAAGAAGCTCATGGCACGGATGAGGATGTGGATGCTGATTCTGCACTGTCTGAAGAGACTATCCAAAAAGTGCTGGCCGGAGGTGAAGACGAACCAATGTCACCAGAAGATGATCAAGAAAGCAGTCAAGCAAGTGAGATTCCACCAGGTGCTGAGACACCGCTGCCTCCCCTCAGCAAGCATAGTTCCGCGAAGCCATCACCATTCTTAGCTGTTCATCTGGTCGACATTGTATACAGCTACTGCATCGCTCTTCGTCTCTACAATGGTGATTGGCAATCAGATGCCACAGGCTCAGCTATGGTCGTTCTCTCCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHQ1 zf-HIT

Protein Analysis

199

Amino Acids

21.9

Weight (kDa)

4.61

Isoelectric Point (pI)

63.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 22 - 51 1.7e-10 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 504, 513
AccII CGCG 1 cut(s) 470
AciI CCGC 4 cut(s) 5, 64, 440, 468
AcoI YGGCCR 1 cut(s) 354
AcuI CTGAAG 3 cut(s) 107, 297, 351
AcvI CACGTG 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 428
AfaI GTAC 2 cut(s) 104, 172
AflIII ACRYGT 2 cut(s) 105, 107
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 5 cut(s) 208, 287, 491, 519, 578
AluI AGCT 5 cut(s) 208, 287, 491, 519, 578
Alw26I GTCTC 4 cut(s) 159, 329, 428, 542
AlwNI CAGNNNCTG 2 cut(s) 431, 522
AoxI GGCC 2 cut(s) 221, 354
ApeKI GCWGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 5 cut(s) 10, 372, 374, 471, 560
BbrPI CACGTG 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 372
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 429
BccI CCATC 2 cut(s) 244, 484
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 4 cut(s) 159, 329, 428, 542
BfaI CTAG 1 cut(s) 209
BisI GCNGC 1 cut(s) 443
BlpI GCTNAGC 1 cut(s) 574
BlsI GCNGC 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 426
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 2 cut(s) 30, 222
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 5 cut(s) 75, 107, 301, 534, 553
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bpu10I CCTNAGC 1 cut(s) 452
Bpu1102I GCTNAGC 1 cut(s) 574
BsaAI YACGTR 1 cut(s) 108
BseBI CCWGG 1 cut(s) 426
BseGI GGATG 3 cut(s) 304, 310, 316
BseMII CTCAG 4 cut(s) 15, 423, 466, 588
BseXI GCAGC 1 cut(s) 429
BsgI GTGCAG 1 cut(s) 306
Bsh1236I CGCG 1 cut(s) 470
BshFI GGCC 2 cut(s) 223, 356
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 28
BsmAI GTCTC 4 cut(s) 159, 329, 428, 542
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 2 cut(s) 223, 356
Bsp143I GATC 1 cut(s) 391
Bsp1720I GCTNAGC 1 cut(s) 574
BspACI CCGC 4 cut(s) 5, 64, 440, 468
BspANI GGCC 2 cut(s) 223, 356
BspCNI CTCAG 4 cut(s) 16, 424, 465, 587
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 30, 222
BspQI GCTCTTC 1 cut(s) 537
BssMI GATC 1 cut(s) 391
BssNAI GTATAC 1 cut(s) 514
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 102, 134, 327
Bst6I CTCTTC 3 cut(s) 144, 327, 537
BstAPI GCANNNNNTGC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 354, 458
BstDEI CTNAG 5 cut(s) 24, 432, 452, 487, 574
BstF5I GGATG 3 cut(s) 304, 310, 316
BstFNI CGCG 1 cut(s) 470
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 4 cut(s) 159, 329, 428, 542
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 6 cut(s) 72, 81, 126, 205, 220, 320
BstNI CCWGG 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 424
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 1 cut(s) 429
BstV2I GAAGAC 1 cut(s) 372
BstZ17I GTATAC 1 cut(s) 514
BsuRI GGCC 2 cut(s) 223, 356
BtgZI GCGATG 1 cut(s) 511
BtsCI GGATG 3 cut(s) 304, 310, 316
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 2 cut(s) 142, 323
Cac8I GCNNGC 2 cut(s) 354, 458
CaiI CAGNNNCTG 2 cut(s) 431, 522
Cfr13I GGNCC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 424
Csp6I GTAC 2 cut(s) 103, 171
CviAII CATG 2 cut(s) 77, 290
CviJI RGCY 9 cut(s) 208, 223, 287, 356, 475, 491, 519, 573, 578
CviKI_1 RGCY 9 cut(s) 208, 223, 287, 356, 475, 491, 519, 573, 578
CviQI GTAC 2 cut(s) 103, 171
DdeI CTNAG 5 cut(s) 24, 432, 452, 487, 574
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
DraIII CACNNNGTG 1 cut(s) 428
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 3 cut(s) 144, 327, 537
EarI CTCTTC 3 cut(s) 144, 327, 537
EciI GGCGGA 1 cut(s) 20
Eco57I CTGAAG 3 cut(s) 107, 297, 351
Eco72I CACGTG 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 2 cut(s) 80, 293
FaiI YATR 9 cut(s) 14, 78, 159, 185, 257, 291, 462, 514, 581
FaqI GGGAC 1 cut(s) 28
FatI CATG 2 cut(s) 76, 289
FauI CCCGC 1 cut(s) 71
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 2 cut(s) 504, 513
Fnu4HI GCNGC 1 cut(s) 443
FokI GGATG 3 cut(s) 311, 317, 323
Fsp4HI GCNGC 1 cut(s) 443
FspBI CTAG 1 cut(s) 209
GluI GCNGC 1 cut(s) 443
HaeIII GGCC 2 cut(s) 223, 356
HapII CCGG 1 cut(s) 357
Hin1II CATG 2 cut(s) 80, 293
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 4 cut(s) 8, 176, 317, 418
HpaII CCGG 1 cut(s) 357
HphI GGTGA 5 cut(s) 10, 372, 374, 471, 560
Hpy166II GTNNAC 3 cut(s) 105, 505, 514
Hpy188I TCNGA 4 cut(s) 25, 277, 331, 562
Hpy188III TCNNGA 1 cut(s) 395
Hpy8I GTNNAC 3 cut(s) 105, 505, 514
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 3 cut(s) 102, 134, 327
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 3 cut(s) 120, 323, 525
HpyF10VI GCNNNNNNNGC 6 cut(s) 72, 81, 126, 205, 220, 320
HpyF3I CTNAG 5 cut(s) 24, 432, 452, 487, 574
HpySE526I ACGT 1 cut(s) 107
Hsp92II CATG 2 cut(s) 80, 293
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 8 cut(s) 98, 338, 370, 396, 411, 438, 485, 555
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 5 cut(s) 75, 107, 301, 534, 553
MabI ACCWGGT 1 cut(s) 424
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 1 cut(s) 378
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 8 cut(s) 131, 256, 290, 293, 344, 377, 398, 524
MfeI CAATTG 1 cut(s) 215
MluCI AATT 1 cut(s) 215
MlyI GAGTC 2 cut(s) 2, 185
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 426
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 426
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 6 cut(s) 72, 81, 126, 205, 220, 320
NdeII GATC 1 cut(s) 391
NlaIII CATG 2 cut(s) 80, 293
NlaIV GGNNCC 2 cut(s) 30, 222
NmuCI GTSAC 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PfeI GAWTC 2 cut(s) 317, 418
PkrI GCNGC 1 cut(s) 444
PleI GAGTC 2 cut(s) 2, 184
PmaCI CACGTG 1 cut(s) 108
PmlI CACGTG 1 cut(s) 108
PpsI GAGTC 2 cut(s) 2, 184
Ppu21I YACGTR 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 424
PspCI CACGTG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 30, 222
PspPI GGNCC 1 cut(s) 221
PstNI CAGNNNCTG 2 cut(s) 431, 522
RsaI GTAC 2 cut(s) 104, 172
RsaNI GTAC 2 cut(s) 103, 171
SalI GTCGAC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 537
SatI GCNGC 1 cut(s) 443
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 2 cut(s) 2, 185
ScrFI CCNGG 1 cut(s) 426
SexAI ACCWGGT 1 cut(s) 424
SfaNI GCATC 5 cut(s) 75, 107, 301, 534, 553
Sse9I AATT 1 cut(s) 215
SsiI CCGC 4 cut(s) 5, 64, 440, 468
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 102, 134, 327
TaiI ACGT 1 cut(s) 110
TaqI TCGA 4 cut(s) 51, 115, 166, 504
TasI AATT 1 cut(s) 215
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 2 cut(s) 317, 418
TscAI CASTG 2 cut(s) 149, 330
TseFI GTSAC 1 cut(s) 378
TseI GCWGC 1 cut(s) 442
Tsp45I GTSAC 1 cut(s) 378
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 2 cut(s) 311, 583
TspRI CASTG 2 cut(s) 149, 330
XmiI GTMKAC 2 cut(s) 504, 513
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.