Rw7G005300

Zinc finger HIT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005386
Physical Location & Seq
Reverse (-)
4509093 .. 4510026
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005300.1

Sequence Viewer

Length: 846 bp
ATGGCGGACTCCATAATCACCTCTGAGAAACCCTCCACCTCGTCCCCTCTCGACCCTCCTTCCCGCATCGTCTGCCACGTTTGCCAGAAGCAGTTTTCACAGTACACGTGTCCTCGATGCAACTCACGCTACTGTTCTCTTCACTGCTACAAGTCGCATAATATTCGATGTACCGAGTCGTTTATGCGAGATAATGTGGTTGGGAAGCTAGGGCAATTGGGGCCGAATGACGAAACGAAGCAGAAGATGATGGACATACTGAAAAGGTTTCACTCTGAAGAAGAAACTATCCAAAAAGTGCTGGCTGGAGGTGAAGTTAGTTTTGATGATCTATCTGCAGAAGAAAAGAAACTATTCCGAAGAGCTGTTGCTTCTGGAGAACTGAGCAAGATGATTAAGCCTTGGGAACCTTGGTGGTTGAAGCCCTCCGTGTCATCGGTATTGGGTCAAGGAGGGCAGCCGGAGACTGTCCTGGAAATTATGGGTGGCTTGCAGTTTGGACTATGTCTCGTAGACGATGTAATAACCGTATTAACATTAGGAACCCCTGCATTGCTGTGCTTGCTTTGTGATCTGCAGAGGATGGTCCAGACCAGGGAGACCGAGCTGAAATCAGAGAATCCACGAAAATCAAGAAGCGCTGAAATTAGGAGTAAGCTGAAGCAGGCCGAAAGGAAAATCTACTTCTTCATGTGTTGGGTCCACGAGCAACCTGGGGAAGCTTGGTCTTCGTTAGGAGCCATCGTAAGGGCAGAGAAAAGTTCAGCTATGAACTATGAACCTTTCTCAAGGTCTGGCAAACTGGTCAACAAAGCCAAACCCAGGAGCAAAGTTTTGATTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHQ1 zf-HIT

Protein Analysis

281

Amino Acids

31.71

Weight (kDa)

8.91

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-HIT PF04438 22 - 51 1.2e-10 HIT zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 513
AciI CCGC 2 cut(s) 5, 64
AcuI CTGAAG 2 cut(s) 297, 680
AcvI CACGTG 1 cut(s) 108
AfaI GTAC 2 cut(s) 104, 172
AfeI AGCGCT 1 cut(s) 640
AfiI CCNNNNNNNGG 3 cut(s) 595, 747, 822
AflIII ACRYGT 2 cut(s) 105, 107
AgsI TTSAA 2 cut(s) 421, 842
AjnI CCWGG 4 cut(s) 471, 593, 712, 821
AluBI AGCT 6 cut(s) 208, 365, 607, 658, 722, 767
AluI AGCT 6 cut(s) 208, 365, 607, 658, 722, 767
Alw26I GTCTC 3 cut(s) 458, 512, 593
Aor51HI AGCGCT 1 cut(s) 640
AoxI GGCC 2 cut(s) 221, 666
ApeKI GCWGC 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 3 cut(s) 221, 586, 700
AsuHPI GGTGA 2 cut(s) 10, 323
AvaII GGWCC 2 cut(s) 586, 700
BauI CACGAG 1 cut(s) 704
BbrPI CACGTG 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 720
BbvI GCAGC 1 cut(s) 469
BccI CCATC 3 cut(s) 244, 577, 749
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 4 cut(s) 473, 595, 714, 823
BcoDI GTCTC 3 cut(s) 458, 512, 593
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 2 cut(s) 336, 575
BfoI RGCGCY 1 cut(s) 642
BisI GCNGC 1 cut(s) 458
BlsI GCNGC 1 cut(s) 459
Bme1390I CCNGG 4 cut(s) 473, 595, 714, 823
Bme18I GGWCC 2 cut(s) 586, 700
BmgT120I GGNCC 3 cut(s) 221, 586, 700
BmiI GGNNCC 5 cut(s) 222, 408, 544, 701, 739
BmrFI CCNGG 4 cut(s) 473, 595, 714, 823
BmsI GCATC 2 cut(s) 75, 107
BpiI GAAGAC 1 cut(s) 720
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 2 cut(s) 327, 396
BpuEI CTTGAG 1 cut(s) 772
BsaAI YACGTR 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 593
BsaJI CCNNGG 5 cut(s) 401, 410, 594, 713, 821
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 729, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 595, 747, 822
Bse1I ACTGG 1 cut(s) 807
Bse3DI GCAATG 1 cut(s) 551
BseBI CCWGG 4 cut(s) 473, 595, 714, 823
BseDI CCNNGG 5 cut(s) 401, 410, 594, 713, 821
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 595, 747, 822
BseMI GCAATG 1 cut(s) 551
BseMII CTCAG 2 cut(s) 15, 374
BseNI ACTGG 1 cut(s) 807
BseXI GCAGC 1 cut(s) 469
BshFI GGCC 2 cut(s) 223, 668
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 3 cut(s) 595, 747, 822
BsmAI GTCTC 3 cut(s) 458, 512, 593
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 2 cut(s) 223, 668
Bso31I GGTCTC 1 cut(s) 593
Bsp143I GATC 2 cut(s) 328, 571
BspACI CCGC 2 cut(s) 5, 64
BspANI GGCC 2 cut(s) 223, 668
BspCNI CTCAG 2 cut(s) 16, 375
BspLI GGNNCC 5 cut(s) 222, 408, 544, 701, 739
BspMAI CTGCAG 2 cut(s) 340, 579
BspQI GCTCTTC 1 cut(s) 355
BspTNI GGTCTC 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 551
BsrI ACTGG 1 cut(s) 807
BssECI CCNNGG 5 cut(s) 401, 410, 594, 713, 821
BssMI GATC 2 cut(s) 328, 571
BssSI CACGAG 1 cut(s) 704
BssT1I CCWWGG 2 cut(s) 401, 410
Bst2BI CACGAG 1 cut(s) 704
Bst2UI CCWGG 4 cut(s) 473, 595, 714, 823
Bst4CI ACNGT 4 cut(s) 102, 134, 469, 529
Bst6I CTCTTC 2 cut(s) 144, 355
BstAPI GCANNNNNTGC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 108
BstC8I GCNNGC 4 cut(s) 303, 491, 563, 666
BstDEI CTNAG 2 cut(s) 24, 383
BstF5I GGATG 1 cut(s) 588
BstH2I RGCGCY 1 cut(s) 642
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 2 cut(s) 331, 574
BstMAI GTCTC 3 cut(s) 458, 512, 593
BstMBI GATC 2 cut(s) 328, 571
BstMWI GCNNNNNNNGC 5 cut(s) 72, 81, 126, 220, 562
BstNI CCWGG 4 cut(s) 473, 595, 714, 823
BstSCI CCNGG 4 cut(s) 471, 593, 712, 821
BstSFI CTRYAG 2 cut(s) 336, 575
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 1 cut(s) 720
BsuRI GGCC 2 cut(s) 223, 668
BtsCI GGATG 1 cut(s) 588
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 142
Cac8I GCNNGC 4 cut(s) 303, 491, 563, 666
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 3 cut(s) 221, 586, 700
Csp6I GTAC 2 cut(s) 103, 171
CviAII CATG 1 cut(s) 691
CviQI GTAC 2 cut(s) 103, 171
DdeI CTNAG 2 cut(s) 24, 383
DpnI GATC 2 cut(s) 330, 573
DpnII GATC 2 cut(s) 328, 571
Eam1104I CTCTTC 2 cut(s) 144, 355
EarI CTCTTC 2 cut(s) 144, 355
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 401, 410
Eco31I GGTCTC 1 cut(s) 593
Eco47I GGWCC 2 cut(s) 586, 700
Eco47III AGCGCT 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 297, 680
Eco72I CACGTG 1 cut(s) 108
EcoRII CCWGG 4 cut(s) 471, 593, 712, 821
EcoT14I CCWWGG 2 cut(s) 401, 410
ErhI CCWWGG 2 cut(s) 401, 410
FaeI CATG 1 cut(s) 694
FaiI YATR 9 cut(s) 14, 159, 185, 257, 482, 505, 692, 770, 777
FaqI GGGAC 1 cut(s) 28
FatI CATG 1 cut(s) 690
FauI CCCGC 1 cut(s) 71
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 1 cut(s) 458
FokI GGATG 1 cut(s) 595
Fsp4HI GCNGC 1 cut(s) 458
FspBI CTAG 1 cut(s) 209
GlaI GCGC 1 cut(s) 640
GluI GCNGC 1 cut(s) 458
GsuI CTGGAG 2 cut(s) 327, 396
HaeII RGCGCY 1 cut(s) 642
HaeIII GGCC 2 cut(s) 223, 668
HapII CCGG 1 cut(s) 461
HhaI GCGC 1 cut(s) 641
Hin1II CATG 1 cut(s) 694
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 1 cut(s) 720
HinfI GANTC 4 cut(s) 8, 176, 619, 838
HpaII CCGG 1 cut(s) 461
HphI GGTGA 2 cut(s) 10, 323
Hpy166II GTNNAC 4 cut(s) 105, 514, 703, 808
Hpy188I TCNGA 4 cut(s) 25, 277, 359, 616
Hpy188III TCNNGA 4 cut(s) 50, 375, 589, 633
Hpy8I GTNNAC 4 cut(s) 105, 514, 703, 808
HpyAV CCTTC 1 cut(s) 69
HpyCH4III ACNGT 4 cut(s) 102, 134, 469, 529
HpyCH4IV ACGT 2 cut(s) 78, 107
HpyCH4V TGCA 5 cut(s) 120, 338, 493, 551, 577
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 81, 126, 220, 562
HpyF3I CTNAG 2 cut(s) 24, 383
HpySE526I ACGT 2 cut(s) 78, 107
Hsp92II CATG 1 cut(s) 694
HspAI GCGC 1 cut(s) 639
Kzo9I GATC 2 cut(s) 328, 571
LguI GCTCTTC 1 cut(s) 355
LmnI GCTCC 2 cut(s) 737, 825
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 2 cut(s) 75, 107
MaeI CTAG 1 cut(s) 209
MaeII ACGT 2 cut(s) 78, 107
MalI GATC 2 cut(s) 330, 573
MboI GATC 2 cut(s) 328, 571
MboII GAAGA 8 cut(s) 131, 256, 290, 293, 353, 372, 679, 720
MfeI CAATTG 1 cut(s) 215
MluCI AATT 3 cut(s) 215, 477, 645
MlyI GAGTC 2 cut(s) 2, 185
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 396, 533
MslI CAYNNNNRTG 1 cut(s) 556
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 4 cut(s) 473, 595, 714, 823
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 4 cut(s) 473, 595, 714, 823
MwoI GCNNNNNNNGC 5 cut(s) 72, 81, 126, 220, 562
NdeII GATC 2 cut(s) 328, 571
NlaIII CATG 1 cut(s) 694
NlaIV GGNNCC 5 cut(s) 222, 408, 544, 701, 739
PciSI GCTCTTC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 619, 838
PflFI GACNNNGTC 1 cut(s) 504
PfoI TCCNGGA 1 cut(s) 471
PkrI GCNGC 1 cut(s) 459
PleI GAGTC 2 cut(s) 2, 184
PmaCI CACGTG 1 cut(s) 108
PmlI CACGTG 1 cut(s) 108
PpsI GAGTC 2 cut(s) 2, 184
Ppu21I YACGTR 1 cut(s) 108
Psp6I CCWGG 4 cut(s) 471, 593, 712, 821
PspCI CACGTG 1 cut(s) 108
PspGI CCWGG 4 cut(s) 471, 593, 712, 821
PspN4I GGNNCC 5 cut(s) 222, 408, 544, 701, 739
PspPI GGNCC 3 cut(s) 221, 586, 700
PstI CTGCAG 2 cut(s) 340, 579
PsyI GACNNNGTC 1 cut(s) 504
RsaI GTAC 2 cut(s) 104, 172
RsaNI GTAC 2 cut(s) 103, 171
RseI CAYNNNNRTG 1 cut(s) 556
SapI GCTCTTC 1 cut(s) 355
SaqAI TTAA 2 cut(s) 396, 533
SatI GCNGC 1 cut(s) 458
Sau3AI GATC 2 cut(s) 328, 571
Sau96I GGNCC 3 cut(s) 221, 586, 700
SchI GAGTC 2 cut(s) 2, 185
ScrFI CCNGG 4 cut(s) 473, 595, 714, 823
SfaNI GCATC 2 cut(s) 75, 107
SfcI CTRYAG 2 cut(s) 336, 575
SinI GGWCC 2 cut(s) 586, 700
SmiMI CAYNNNNRTG 1 cut(s) 556
SmlI CTYRAG 1 cut(s) 787
SmoI CTYRAG 1 cut(s) 787
Sse9I AATT 3 cut(s) 215, 477, 645
SsiI CCGC 2 cut(s) 5, 64
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 4 cut(s) 471, 593, 712, 821
StyI CCWWGG 2 cut(s) 401, 410
TaaI ACNGT 4 cut(s) 102, 134, 469, 529
TaiI ACGT 2 cut(s) 81, 110
TaqI TCGA 3 cut(s) 51, 115, 166
TaqII GACCGA 1 cut(s) 617
TasI AATT 3 cut(s) 215, 477, 645
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 2 cut(s) 619, 838
Tru1I TTAA 2 cut(s) 396, 533
Tru9I TTAA 2 cut(s) 396, 533
TscAI CASTG 1 cut(s) 149
TseI GCWGC 1 cut(s) 457
TspDTI ATGAA 3 cut(s) 679, 785, 792
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 1 cut(s) 149
Tth111I GACNNNGTC 1 cut(s) 504
VpaK11BI GGWCC 2 cut(s) 586, 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.