MD06G1180800.v1.1
ERF Family

HhH-GPD superfamily base excision DNA repair protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003200
Physical Location & Seq
Reverse (-)
32018949 .. 32020990
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1180800.v1.1.491

Sequence Viewer

Length: 864 bp
ATGCCGAAAACTCGCAAAAGAAAGCACGTGGAGGCTGAGCAGAAGCCAAAATCCGCAACCAAATCAGCCAAAACCGCCAATCCCTTCAAATCGACGGCAAAGGACCCATACCCGAATCACCCCCGACCCACCGGAGAAGAATGCTTGTCCGTACGCGACGACCTCTTGGCCTTCCACGGCTTCCCGGAAGAGTTCGCCGAGTACAGAAAGCAGAGACTGATGGCTCTTGAAACCGACGGCGCCATGAATTCAGAGCCGTCAGATCGGAAAGAGAGTGTTTTGGATGGGTTGGTGAGGACGCTGCTGTCGCAGAACACCACGGAAGTCAATTCCCAAAAGGCTTTTGCTTCTCTCAAATCAGCTTTTCCGACTTGGGAAGATGTTCTTGGTGCTGATTCAAATTCCATAGAGAATGCCATACGATGTGGAGGCCTAGCCAGGACTAAAACGTCGTGTATTAAGAACATGTTGAGGTGTTTACTGGAGAAGAAAGAAAAGTTGTGCTTGGAGTACTTGAGAGAGTTGTCAGTTGACGAAATTAAGTCCGAGCTATCTTGTTTCAAAGGAATCGGTCCCAAGACGGTGGCTTGTGTTTTGATGTTCCAGCTTCAGCAAGACGATTTTCCGGTGGACACTCATGTGTTCGAGATTGCAAAGGCTATTGGTTGGGTGCCAGCTGAAGCGGACAGGAATAAGACGTATCTTCATCTCAACCAATGGATCCCGAACGAACTCAAATTTGACCTCAACTGCCTTCTGTATACTCACGGCAAGCTCTGCCGCAAATGCATCAAGAAAGGGGATAGCCAGCAAGGAAAGGAATCCCATGATAACTCCTGTCCTTTATTACGTTACTGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
HhH-GPD

Protein Analysis

288

Amino Acids

32.49

Weight (kDa)

8.51

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HhH-GPD PF00730 98 - 242 3e-13 HhH-GPD superfamily base excision DNA repair protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 304, 448
AccB1I GGYRCC 2 cut(s) 239, 670
AccI GTMKAC 1 cut(s) 761
AccII CGCG 1 cut(s) 156
AciI CCGC 4 cut(s) 54, 75, 683, 781
AclWI GGATC 2 cut(s) 715, 728
AcsI RAATTY 3 cut(s) 247, 400, 737
AcuI CTGAAG 2 cut(s) 593, 699
AcvI CACGTG 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 3 cut(s) 153, 203, 512
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 4 cut(s) 88, 230, 399, 562
AjnI CCWGG 1 cut(s) 437
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AleI CACNNNNGTG 1 cut(s) 638
AluBI AGCT 5 cut(s) 362, 550, 607, 677, 775
AluI AGCT 5 cut(s) 362, 550, 607, 677, 775
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 2 cut(s) 715, 728
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 2 cut(s) 168, 430
ApeKI GCWGC 1 cut(s) 301
ApoI RAATTY 3 cut(s) 247, 400, 737
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 103, 572
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 110, 304
AvaII GGWCC 2 cut(s) 103, 572
BamHI GGATCC 1 cut(s) 720
BanI GGYRCC 2 cut(s) 239, 670
BbrPI CACGTG 1 cut(s) 28
BbvI GCAGC 1 cut(s) 288
BccI CCATC 2 cut(s) 214, 278
BceAI ACGGC 5 cut(s) 111, 193, 241, 253, 784
BciT130I CCWGG 1 cut(s) 439
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 1 cut(s) 434
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 2 cut(s) 302, 781
BlpI GCTNAGC 1 cut(s) 36
BlsI GCNGC 2 cut(s) 303, 782
BmcAI AGTACT 1 cut(s) 512
Bme1390I CCNGG 2 cut(s) 185, 439
Bme18I GGWCC 2 cut(s) 103, 572
BmgT120I GGNCC 2 cut(s) 103, 572
BmiI GGNNCC 5 cut(s) 105, 241, 574, 672, 722
BmrFI CCNGG 2 cut(s) 185, 439
BmsI GCATC 1 cut(s) 798
BpmI CTGGAG 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 535
BpuMI CCSGG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 175, 318
BsaWI WCCGGW 2 cut(s) 131, 625
Bse1I ACTGG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 175, 318
BseGI GGATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 1 cut(s) 486
BseXI GCAGC 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 170, 432
BshNI GGYRCC 2 cut(s) 239, 670
BsiSI CCGG 3 cut(s) 132, 185, 626
BsiWI CGTACG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 558
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 558
BsmI GAATGC 2 cut(s) 146, 418
BsnI GGCC 2 cut(s) 170, 432
Bsp143I GATC 2 cut(s) 262, 720
Bsp1720I GCTNAGC 1 cut(s) 36
BspACI CCGC 4 cut(s) 54, 75, 683, 781
BspANI GGCC 2 cut(s) 170, 432
BspCNI CTCAG 1 cut(s) 28
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 5 cut(s) 105, 241, 574, 672, 722
BspPI GGATC 2 cut(s) 715, 728
BspT107I GGYRCC 2 cut(s) 239, 670
BsrI ACTGG 1 cut(s) 486
BssECI CCNNGG 2 cut(s) 175, 318
BssMI GATC 2 cut(s) 262, 720
BssNAI GTATAC 1 cut(s) 762
BssNI GRCGYC 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 762
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 2 cut(s) 583, 857
Bst6I CTCTTC 1 cut(s) 183
BstACI GRCGYC 1 cut(s) 240
BstAPI GCANNNNNTGC 1 cut(s) 777
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 675, 773, 809
BstDEI CTNAG 1 cut(s) 36
BstDSI CCRYGG 2 cut(s) 175, 318
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 2 cut(s) 265, 723
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 2 cut(s) 262, 720
BstMWI GCNNNNNNNGC 4 cut(s) 74, 307, 777, 786
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 183, 437
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 288
BstX2I RGATCY 1 cut(s) 720
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 720
BstZ17I GTATAC 1 cut(s) 762
BsuRI GGCC 2 cut(s) 170, 432
BtgI CCRYGG 2 cut(s) 175, 318
BtsCI GGATG 1 cut(s) 289
Cac8I GCNNGC 3 cut(s) 675, 773, 809
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 103, 572
CseI GACGC 1 cut(s) 307
Csp6I GTAC 3 cut(s) 152, 202, 511
CviAII CATG 4 cut(s) 244, 466, 638, 827
CviQI GTAC 3 cut(s) 152, 202, 511
DdeI CTNAG 1 cut(s) 36
DinI GGCGCC 1 cut(s) 241
DpnI GATC 2 cut(s) 264, 722
DpnII GATC 2 cut(s) 262, 720
DrdI GACNNNNNNGTC 2 cut(s) 304, 448
DseDI GACNNNNNNGTC 2 cut(s) 304, 448
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 432
Eco47I GGWCC 2 cut(s) 103, 572
Eco57I CTGAAG 2 cut(s) 593, 699
Eco72I CACGTG 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 103
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 437
EcoT22I ATGCAT 1 cut(s) 791
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
FaeI CATG 4 cut(s) 247, 469, 641, 830
FaiI YATR 8 cut(s) 109, 245, 407, 419, 467, 639, 762, 828
FalI AAGNNNNNCTT 4 cut(s) 369, 401, 488, 520
FaqI GGGAC 1 cut(s) 558
FatI CATG 4 cut(s) 243, 465, 637, 826
FblI GTMKAC 1 cut(s) 761
Fnu4HI GCNGC 2 cut(s) 302, 781
FokI GGATG 1 cut(s) 296
Fsp4HI GCNGC 2 cut(s) 302, 781
FspBI CTAG 1 cut(s) 434
GlaI GCGC 1 cut(s) 241
GluI GCNGC 2 cut(s) 302, 781
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 2 cut(s) 170, 432
HapII CCGG 3 cut(s) 132, 185, 626
HgaI GACGC 1 cut(s) 307
HhaI GCGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 4 cut(s) 247, 469, 641, 830
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 4 cut(s) 115, 395, 567, 821
HpaII CCGG 3 cut(s) 132, 185, 626
HphI GGTGA 2 cut(s) 110, 304
Hpy166II GTNNAC 4 cut(s) 479, 532, 631, 762
Hpy188I TCNGA 5 cut(s) 253, 262, 267, 369, 547
Hpy188III TCNNGA 4 cut(s) 227, 646, 724, 793
Hpy8I GTNNAC 4 cut(s) 479, 532, 631, 762
Hpy99I CGWCG 4 cut(s) 97, 161, 239, 454
HpyAV CCTTC 3 cut(s) 94, 181, 764
HpyCH4III ACNGT 2 cut(s) 583, 857
HpyCH4IV ACGT 4 cut(s) 27, 449, 698, 850
HpyCH4V TGCA 2 cut(s) 653, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 307, 777, 786
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 4 cut(s) 27, 449, 698, 850
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 4 cut(s) 247, 469, 641, 830
HspAI GCGC 1 cut(s) 240
KasI GGCGCC 1 cut(s) 239
Kzo9I GATC 2 cut(s) 262, 720
Lsp1109I GCAGC 1 cut(s) 288
LweI GCATC 1 cut(s) 798
MaeI CTAG 1 cut(s) 434
MaeII ACGT 4 cut(s) 27, 449, 698, 850
MaeIII GTNAC 1 cut(s) 851
MalI GATC 2 cut(s) 264, 722
MboI GATC 2 cut(s) 262, 720
MboII GAAGA 5 cut(s) 149, 200, 389, 499, 695
MflI RGATCY 1 cut(s) 720
MluCI AATT 5 cut(s) 247, 328, 400, 537, 737
Mly113I GGCGCC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 6 cut(s) 25, 173, 288, 422, 465, 755
Mph1103I ATGCAT 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 2 cut(s) 459, 540
MslI CAYNNNNRTG 1 cut(s) 638
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 3 cut(s) 132, 185, 626
MspR9I CCNGG 2 cut(s) 185, 439
Mva1269I GAATGC 2 cut(s) 146, 418
MvaI CCWGG 1 cut(s) 439
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 4 cut(s) 74, 307, 777, 786
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 1 cut(s) 185
NdeII GATC 2 cut(s) 262, 720
NlaIII CATG 4 cut(s) 247, 469, 641, 830
NlaIV GGNNCC 5 cut(s) 105, 241, 574, 672, 722
NmeAIII GCCGAG 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 791
NspI RCATGY 1 cut(s) 469
OliI CACNNNNGTG 1 cut(s) 638
PceI AGGCCT 1 cut(s) 432
PciI ACATGT 1 cut(s) 465
PctI GAATGC 2 cut(s) 146, 418
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 4 cut(s) 115, 395, 567, 821
Pfl23II CGTACG 1 cut(s) 151
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 2 cut(s) 303, 782
PluTI GGCGCC 1 cut(s) 243
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 103
PscI ACATGT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 437
PspCI CACGTG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 437
PspLI CGTACG 1 cut(s) 151
PspN4I GGNNCC 5 cut(s) 105, 241, 574, 672, 722
PspPI GGNCC 2 cut(s) 103, 572
PspPPI RGGWCCY 1 cut(s) 103
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 720
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 3 cut(s) 153, 203, 512
RsaNI GTAC 3 cut(s) 152, 202, 511
RseI CAYNNNNRTG 1 cut(s) 638
SaqAI TTAA 2 cut(s) 459, 540
SatI GCNGC 2 cut(s) 302, 781
Sau3AI GATC 2 cut(s) 262, 720
Sau96I GGNCC 2 cut(s) 103, 572
ScaI AGTACT 1 cut(s) 512
ScrFI CCNGG 2 cut(s) 185, 439
SfaNI GCATC 1 cut(s) 798
SfoI GGCGCC 1 cut(s) 241
SinI GGWCC 2 cut(s) 103, 572
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 514
SmoI CTYRAG 1 cut(s) 514
Sse9I AATT 5 cut(s) 247, 328, 400, 537, 737
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 4 cut(s) 54, 75, 683, 781
SspDI GGCGCC 1 cut(s) 239
SspMI CTAG 1 cut(s) 434
StuI AGGCCT 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 183, 437
TaaI ACNGT 2 cut(s) 583, 857
TaiI ACGT 4 cut(s) 30, 452, 701, 853
TaqI TCGA 2 cut(s) 92, 645
TaqII GACCGA 1 cut(s) 560
TasI AATT 5 cut(s) 247, 328, 400, 537, 737
TatI WGTACW 2 cut(s) 201, 510
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 4 cut(s) 115, 395, 567, 821
Tru1I TTAA 2 cut(s) 459, 540
Tru9I TTAA 2 cut(s) 459, 540
TseI GCWGC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 260, 695
TspGWI ACGGA 2 cut(s) 139, 335
VpaK11BI GGWCC 2 cut(s) 103, 572
XapI RAATTY 3 cut(s) 247, 400, 737
XceI RCATGY 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.