MD07G1002000.v1.1

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006955
Physical Location & Seq
Forward (+)
252870 .. 255079
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1002000.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGGTTATGGAAATTGGTTTGGAAAAGGGGTTCCGCACTGCACTTAGCGATTTTGTATTGATGCAATTACAATTGGCCCCGGTGTTTTTCACATTTTCTCTTGGTACAAAGACCCACTATTATGGAAAGACTTTGCTTCATGGAGGTGCAGAATACAGAGGAACGGGTCGTAGCTTTGTAGTGTTCCATGCCAAATTTGCTGATAACTATAGACTCTACTCTAGAAGTCACTTTGTCAAGGGCATTGAGTTACTGATTCTGCTTGTTGTGTACCACCTATTTGGTCGTTCATATAAAAGTGGAGTTGTATATATCCTCATTACAATACAGATATGGTTCATGGTTGGAACATGGCTTTTTGCTCCTTTCCTCTTCAATCCATCTGGGTTTGAGTGGCAAAAGATCGTCGATGACTGGACAGATTGGAAAAAGTGGATAAACAATCATGGAGGGATCGGTGTATCACCTGAAAAAAGTTGGGAATCCTGGTGGGAAAAAGAACATGAGCATCTTCTTTACTCTGGGGTGCGTGGTATCGTTGCTGAGATATTATTAGCATTGCGCTTTTTCATTTACCAGTATGGGCTTGTTTATCACCTTAACATCACAAACAACAAAAGTTTCCTGGTATATGGTGTTTCGTGGCTTGTTATCATTTTGATTTTGTGTCTGATGAAGGCTGTGTCTGCTGGAAGGCGACGATTAAGTGCAGATTATCAGCTTTTGTTTCGACTGGTTAAGGGATTTATATTTATTACATTTTTAGCTATATTCGTTACCTTGATAGTGCTCCCTCATATGACGCTTCGGGATGTCGTAGTGTGCATTCTTGCCTTCATGCCAACTGGTTGGGGATTGCTTTTGATTGCGCAAGCTTGTAAGCCGGTAATTAAACGAGCAGGGTTTTGGGGATCAGTTGAAACACTTGCTCGTGGTTATGAAATAATCATGGGGTTGCTTCTTTTTACCCCGGTTGCATTCTTGGCTTGGTTTCCGTTTGTTTCCGAATTCCAGACGCGAATGCTTTTCAACCAAGCATTCAGTAGAGGTTTACAGATCTCGCGTATTCTTGGAGGACAAAGGAAGGGCCCTCACTCCTCCAACAATAATGAATAG

Protein Analysis

372

Amino Acids

42.69

Weight (kDa)

9.68

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucan_synthase PF02364 1 - 178 2.5e-44 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 870
AccII CGCG 2 cut(s) 1018, 1063
AciI CCGC 1 cut(s) 34
AclWI GGATC 2 cut(s) 461, 919
AcsI RAATTY 2 cut(s) 194, 1007
AfaI GTAC 2 cut(s) 106, 272
AgsI TTSAA 3 cut(s) 376, 920, 1030
AjnI CCWGG 2 cut(s) 485, 624
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
AluBI AGCT 4 cut(s) 174, 721, 767, 875
AluI AGCT 4 cut(s) 174, 721, 767, 875
Alw21I GWGCWC 1 cut(s) 792
AlwI GGATC 2 cut(s) 461, 919
AoxI GGCC 2 cut(s) 75, 1087
ApaI GGGCCC 1 cut(s) 1091
ApoI RAATTY 2 cut(s) 194, 1007
AspLEI GCGC 2 cut(s) 564, 871
AspS9I GGNCC 3 cut(s) 76, 1087, 1088
AsuC2I CCSGG 2 cut(s) 80, 971
AsuHPI GGTGA 2 cut(s) 456, 587
BaeGI GKGCMC 1 cut(s) 1091
BanII GRGCYC 1 cut(s) 1091
BauI CACGAG 1 cut(s) 930
Bbv12I GWGCWC 1 cut(s) 792
BccI CCATC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 487, 626
BcnI CCSGG 2 cut(s) 80, 971
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 208
BglII AGATCT 1 cut(s) 1056
Bme1390I CCNGG 4 cut(s) 80, 487, 626, 971
BmgT120I GGNCC 3 cut(s) 76, 1087, 1088
BmiI GGNNCC 3 cut(s) 32, 78, 1089
BmrFI CCNGG 4 cut(s) 80, 487, 626, 971
BmsI GCATC 2 cut(s) 51, 517
BpuMI CCSGG 2 cut(s) 80, 971
BsaJI CCNNGG 2 cut(s) 78, 969
Bse118I RCCGGY 1 cut(s) 883
Bse1I ACTGG 4 cut(s) 419, 577, 738, 850
Bse3DI GCAATG 1 cut(s) 557
BseBI CCWGG 2 cut(s) 487, 626
BseDI CCNNGG 2 cut(s) 78, 969
BseGI GGATG 1 cut(s) 817
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 4 cut(s) 419, 577, 738, 850
BseRI GAGGAG 1 cut(s) 1087
BseSI GKGCMC 1 cut(s) 1091
BsgI GTGCAG 3 cut(s) 24, 168, 729
Bsh1236I CGCG 2 cut(s) 1018, 1063
BshFI GGCC 2 cut(s) 77, 1089
BsiHKAI GWGCWC 1 cut(s) 792
BsiSI CCGG 3 cut(s) 80, 884, 971
BsmI GAATGC 4 cut(s) 825, 977, 1026, 1037
BsnI GGCC 2 cut(s) 77, 1089
Bsp120I GGGCCC 1 cut(s) 1087
Bsp1286I GDGCHC 2 cut(s) 792, 1091
Bsp143I GATC 4 cut(s) 402, 453, 911, 1056
BspACI CCGC 1 cut(s) 34
BspANI GGCC 2 cut(s) 77, 1089
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 2 cut(s) 1018, 1063
BspLI GGNNCC 3 cut(s) 32, 78, 1089
BspPI GGATC 2 cut(s) 461, 919
BsrDI GCAATG 1 cut(s) 557
BsrFI RCCGGY 1 cut(s) 883
BsrI ACTGG 4 cut(s) 419, 577, 738, 850
BssAI RCCGGY 1 cut(s) 883
BssECI CCNNGG 2 cut(s) 78, 969
BssMI GATC 4 cut(s) 402, 453, 911, 1056
BssSI CACGAG 1 cut(s) 930
Bst2BI CACGAG 1 cut(s) 930
Bst2UI CCWGG 2 cut(s) 487, 626
Bst6I CTCTTC 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 873
BstDEI CTNAG 2 cut(s) 44, 543
BstF5I GGATG 1 cut(s) 817
BstFNI CGCG 2 cut(s) 1018, 1063
BstHHI GCGC 2 cut(s) 564, 871
BstKTI GATC 4 cut(s) 405, 456, 914, 1059
BstMBI GATC 4 cut(s) 402, 453, 911, 1056
BstMWI GCNNNNNNNGC 3 cut(s) 197, 686, 983
BstNI CCWGG 2 cut(s) 487, 626
BstSCI CCNGG 4 cut(s) 78, 485, 624, 969
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 1091
BstUI CGCG 2 cut(s) 1018, 1063
BstX2I RGATCY 1 cut(s) 1056
BstXI CCANNNNNNTGG 3 cut(s) 122, 281, 849
BstYI RGATCY 1 cut(s) 1056
BsuRI GGCC 2 cut(s) 77, 1089
BtsCI GGATG 1 cut(s) 817
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 873
CfoI GCGC 2 cut(s) 564, 871
Cfr10I RCCGGY 1 cut(s) 883
Cfr13I GGNCC 3 cut(s) 76, 1087, 1088
CseI GACGC 2 cut(s) 811, 1024
Csp6I GTAC 2 cut(s) 105, 271
CviAII CATG 8 cut(s) 140, 188, 340, 351, 446, 503, 838, 949
CviQI GTAC 2 cut(s) 105, 271
DdeI CTNAG 2 cut(s) 44, 543
DpnI GATC 4 cut(s) 404, 455, 913, 1058
DpnII GATC 4 cut(s) 402, 453, 911, 1056
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 1091
EcoO109I RGGNCCY 2 cut(s) 1087, 1088
EcoRI GAATTC 1 cut(s) 1007
EcoRII CCWGG 2 cut(s) 485, 624
EcoT38I GRGCYC 1 cut(s) 1091
FaeI CATG 8 cut(s) 143, 191, 343, 354, 449, 506, 841, 952
FatI CATG 8 cut(s) 139, 187, 339, 350, 445, 502, 837, 948
FauNDI CATATG 1 cut(s) 798
FokI GGATG 1 cut(s) 824
FriOI GRGCYC 1 cut(s) 1091
FspBI CTAG 1 cut(s) 222
FspI TGCGCA 1 cut(s) 870
GlaI GCGC 2 cut(s) 563, 870
HaeIII GGCC 2 cut(s) 77, 1089
HapII CCGG 3 cut(s) 80, 884, 971
HgaI GACGC 2 cut(s) 811, 1024
HhaI GCGC 2 cut(s) 564, 871
Hin1II CATG 8 cut(s) 143, 191, 343, 354, 449, 506, 841, 952
Hin6I GCGC 2 cut(s) 562, 869
HinP1I GCGC 2 cut(s) 562, 869
HindIII AAGCTT 1 cut(s) 873
HinfI GANTC 3 cut(s) 213, 256, 482
HpaII CCGG 3 cut(s) 80, 884, 971
HphI GGTGA 2 cut(s) 456, 587
Hpy166II GTNNAC 2 cut(s) 271, 1052
Hpy188I TCNGA 2 cut(s) 672, 1006
Hpy188III TCNNGA 3 cut(s) 222, 809, 1012
Hpy8I GTNNAC 2 cut(s) 271, 1052
Hpy99I CGWCG 2 cut(s) 410, 702
HpyAV CCTTC 4 cut(s) 670, 687, 844, 1078
HpyCH4V TGCA 6 cut(s) 41, 64, 149, 710, 825, 977
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 686, 983
HpyF3I CTNAG 2 cut(s) 44, 543
Hsp92II CATG 8 cut(s) 143, 191, 343, 354, 449, 506, 841, 952
HspAI GCGC 2 cut(s) 562, 869
Kzo9I GATC 4 cut(s) 402, 453, 911, 1056
LmnI GCTCC 2 cut(s) 367, 795
LweI GCATC 2 cut(s) 51, 517
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 3 cut(s) 227, 249, 775
MalI GATC 4 cut(s) 404, 455, 913, 1058
MboI GATC 4 cut(s) 402, 453, 911, 1056
MboII GAAGA 2 cut(s) 364, 503
MfeI CAATTG 1 cut(s) 71
MflI RGATCY 1 cut(s) 1056
MhlI GDGCHC 2 cut(s) 792, 1091
MluCI AATT 6 cut(s) 12, 65, 71, 194, 888, 1007
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 325
MseI TTAA 4 cut(s) 600, 704, 738, 891
MslI CAYNNNNRTG 2 cut(s) 120, 144
MspI CCGG 3 cut(s) 80, 884, 971
MspR9I CCNGG 4 cut(s) 80, 487, 626, 971
MunI CAATTG 1 cut(s) 71
Mva1269I GAATGC 4 cut(s) 825, 977, 1026, 1037
MvaI CCWGG 2 cut(s) 487, 626
MvnI CGCG 2 cut(s) 1018, 1063
MwoI GCNNNNNNNGC 3 cut(s) 197, 686, 983
NciI CCSGG 2 cut(s) 80, 971
NdeI CATATG 1 cut(s) 798
NdeII GATC 4 cut(s) 402, 453, 911, 1056
NlaIII CATG 8 cut(s) 143, 191, 343, 354, 449, 506, 841, 952
NlaIV GGNNCC 3 cut(s) 32, 78, 1089
NmuCI GTSAC 1 cut(s) 227
NsbI TGCGCA 1 cut(s) 870
PctI GAATGC 4 cut(s) 825, 977, 1026, 1037
PfeI GAWTC 2 cut(s) 256, 482
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 485, 624
PspGI CCWGG 2 cut(s) 485, 624
PspN4I GGNNCC 3 cut(s) 32, 78, 1089
PspOMI GGGCCC 1 cut(s) 1087
PspPI GGNCC 3 cut(s) 76, 1087, 1088
PsuI RGATCY 1 cut(s) 1056
RsaI GTAC 2 cut(s) 106, 272
RsaNI GTAC 2 cut(s) 105, 271
RseI CAYNNNNRTG 2 cut(s) 120, 144
SaqAI TTAA 4 cut(s) 600, 704, 738, 891
Sau3AI GATC 4 cut(s) 402, 453, 911, 1056
Sau96I GGNCC 3 cut(s) 76, 1087, 1088
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 4 cut(s) 80, 487, 626, 971
SduI GDGCHC 2 cut(s) 792, 1091
SfaNI GCATC 2 cut(s) 51, 517
SfcI CTRYAG 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 120, 144
Sse9I AATT 6 cut(s) 12, 65, 71, 194, 888, 1007
SsiI CCGC 1 cut(s) 34
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 4 cut(s) 78, 485, 624, 969
TaqI TCGA 2 cut(s) 408, 730
TasI AATT 6 cut(s) 12, 65, 71, 194, 888, 1007
TfiI GAWTC 2 cut(s) 256, 482
Tru1I TTAA 4 cut(s) 600, 704, 738, 891
Tru9I TTAA 4 cut(s) 600, 704, 738, 891
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 1 cut(s) 227
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 7 cut(s) 128, 279, 328, 559, 689, 826, 954
TspGWI ACGGA 1 cut(s) 984
TspRI CASTG 1 cut(s) 43
XapI RAATTY 2 cut(s) 194, 1007
XbaI TCTAGA 1 cut(s) 221
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.