RchiOBHm_Chr1g0313351

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006955
Physical Location & Seq
Forward (+)
453955 .. 456677
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54417

Sequence Viewer

Length: 1329 bp
ATGTTGTCCTGCTATTTCACCACAGTTGGTTTCTACTTCAGCACCTTGTTAACAGTGCTTACGGTTTATGTGTTTCTCTACGGTCGCCTTTATCTGGTTCTGAGCGGACTTGAAAAGGGGCTGAGTACTCAACGAGCTATTCGAAACAATAAGCCTCTTCAAGTGGCCCTTGCTTCTCAGTCTGTAGTGCAAATTGGTTTTTTAATGGCATTGCCTATGATTATGGAAATTGGATTGGAAAAGGGGTTCCGTACTGCACTAAGTGATTTTATATTAATGCAATTACAATTGGCCCCTGTATTTTTCACATTTTCACTTGGTACAAAGACTCACTATTACGGAAAGACTTTGCTTCATGGAGGTGCAGAGTACAGAGGAACTGGTCGTAGCTTTGTTGTATTTCATGCCAAATTTGCTGATAACTACAGGCTCTACTCCCGAAGCCATTTTGTCAAGGGCATTGAGCTTTTGATTCTGCTTGTTGTGTACCATATATTTGGTCGTTCATATAGAAGTGGAGTTGTATATATTCTCATTACTGTACAGATATGGTTCATGGTTGGAACGTGGCTTTTCGCTCCTTTCCTGTTCAACCCGTCAGGGTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAGAAGTGGATAAGCAATCATGGAGGGATTGGTGTATCTCCTGAAAAAAGTTGGGAATCCTGGTGGGAGAAGGAACACGAGCATCTTCGCTACTCTGGGATGCGCGGTATCATTACTGAGATATTATTAGCATTGCGCTTTTTTGTCTACCAGTATGGGCTTGTATATCACCTCAACATTACAGAGAACAAAAGTATCCTGGTATATGGTGTTTCGTGGCTGGTGATCATCGTTGTTTTGTTGCTGATGAAGGCTGTGTCAGCTGGAAGGCGACGATTAAGTGCAGACTTTCAGCTCTTGTTTCGACTTGTTAAGGGATGTATGTTTATTACACTCTTATCTGTCTTTGTTATCTTAGTAGTGCTCACTCATATGACGATAAAGGATGTCATAGTCTGCATTCTCGCCTTTATGCCAACTGGATGGGGAATGCTTTTGATTGCACAAGCTTGCAAGAAACCAATTAAACGAGCAGGATTTTGGCTGTCGGTTCAAACACTGGCTCGTGGTTATGAAGTAATCATGGGTTTGCTTCTATTTACCCCGGTAGCATTCTTGGCTTGGTTCCCTTTTGTTTCTGAATTCCAAACGCGAATGCTGTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCACGTATTCTTGGAGGGCCTAGGAAGGGCCCCCGCGCTAGCAATAGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0009504 GO:0009506 GO:0016020 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

442

Amino Acids

50.61

Weight (kDa)

9.86

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucan_synthase PF02364 1 - 336 2.8e-57 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 789
AccII CGCG 3 cut(s) 747, 1234, 1311
AciI CCGC 3 cut(s) 105, 747, 1309
AcsI RAATTY 2 cut(s) 410, 1223
AcuI CTGAAG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 6 cut(s) 127, 253, 322, 371, 488, 543
AfiI CCNNNNNNNGG 2 cut(s) 94, 1301
AgsI TTSAA 5 cut(s) 113, 161, 592, 1136, 1246
AjnI CCWGG 2 cut(s) 701, 840
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 6 cut(s) 137, 390, 466, 905, 937, 1091
AluI AGCT 6 cut(s) 137, 390, 466, 905, 937, 1091
Alw21I GWGCWC 1 cut(s) 1008
AoxI GGCC 4 cut(s) 165, 291, 1292, 1303
ApaI GGGCCC 1 cut(s) 1307
ApoI RAATTY 2 cut(s) 410, 1223
AseI ATTAAT 1 cut(s) 275
AspA2I CCTAGG 1 cut(s) 1295
AspLEI GCGC 3 cut(s) 747, 780, 1313
AspS9I GGNCC 5 cut(s) 166, 292, 1292, 1303, 1304
AsuC2I CCSGG 1 cut(s) 1187
AsuHPI GGTGA 3 cut(s) 10, 803, 877
AsuII TTCGAA 1 cut(s) 142
AsuNHI GCTAGC 1 cut(s) 1313
AvrII CCTAGG 1 cut(s) 1295
BaeGI GKGCMC 1 cut(s) 1307
BaeI ACNNNNGTAYC 2 cut(s) 820, 853
BanII GRGCYC 1 cut(s) 1307
BauI CACGAG 2 cut(s) 719, 1146
Bbv12I GWGCWC 1 cut(s) 1008
BccI CCATC 1 cut(s) 1059
BciT130I CCWGG 2 cut(s) 703, 842
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 1187
BfaI CTAG 2 cut(s) 1296, 1314
BfmI CTRYAG 2 cut(s) 183, 424
BfuI GTATCC 1 cut(s) 848
BlnI CCTAGG 1 cut(s) 1295
BmcAI AGTACT 1 cut(s) 127
Bme1390I CCNGG 3 cut(s) 703, 842, 1187
BmgT120I GGNCC 5 cut(s) 166, 292, 1292, 1303, 1304
BmiI GGNNCC 5 cut(s) 248, 294, 1208, 1305, 1306
BmrFI CCNGG 3 cut(s) 703, 842, 1187
BmsI GCATC 2 cut(s) 732, 733
BmtI GCTAGC 1 cut(s) 1317
Bpu14I TTCGAA 1 cut(s) 142
BpuMI CCSGG 1 cut(s) 1187
BsaAI YACGTR 1 cut(s) 1280
BsaJI CCNNGG 2 cut(s) 1185, 1295
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 1301
Bse1I ACTGG 5 cut(s) 385, 635, 793, 1066, 1146
Bse3DI GCAATG 2 cut(s) 209, 773
BseBI CCWGG 2 cut(s) 703, 842
BseDI CCNNGG 2 cut(s) 1185, 1295
BseGI GGATG 4 cut(s) 747, 965, 1033, 1070
BseLI CCNNNNNNNGG 2 cut(s) 94, 1301
BseMI GCAATG 2 cut(s) 209, 773
BseMII CTCAG 4 cut(s) 92, 113, 191, 750
BseNI ACTGG 5 cut(s) 385, 635, 793, 1066, 1146
BseSI GKGCMC 1 cut(s) 1307
BsgI GTGCAG 3 cut(s) 240, 384, 945
Bsh1236I CGCG 3 cut(s) 747, 1234, 1311
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 4 cut(s) 167, 293, 1294, 1305
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 1008
BsiSI CCGG 1 cut(s) 1187
BslI CCNNNNNNNGG 2 cut(s) 94, 1301
BsmI GAATGC 5 cut(s) 1041, 1077, 1193, 1242, 1253
BsnI GGCC 4 cut(s) 167, 293, 1294, 1305
Bsp119I TTCGAA 1 cut(s) 142
Bsp120I GGGCCC 1 cut(s) 1303
Bsp1286I GDGCHC 2 cut(s) 1008, 1307
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 1 cut(s) 867
BspACI CCGC 3 cut(s) 105, 747, 1309
BspANI GGCC 4 cut(s) 167, 293, 1294, 1305
BspCNI CTCAG 4 cut(s) 93, 114, 190, 751
BspFNI CGCG 3 cut(s) 747, 1234, 1311
BspLI GGNNCC 5 cut(s) 248, 294, 1208, 1305, 1306
BspOI GCTAGC 1 cut(s) 1317
BspT104I TTCGAA 1 cut(s) 142
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 2 cut(s) 209, 773
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 5 cut(s) 385, 635, 793, 1066, 1146
BssECI CCNNGG 2 cut(s) 1185, 1295
BssMI GATC 1 cut(s) 867
BssSI CACGAG 2 cut(s) 719, 1146
BssT1I CCWWGG 1 cut(s) 1295
Bst2BI CACGAG 2 cut(s) 719, 1146
Bst2UI CCWGG 2 cut(s) 703, 842
Bst4CI ACNGT 5 cut(s) 25, 55, 64, 83, 541
Bst6I CTCTTC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 541
BstBAI YACGTR 1 cut(s) 1280
BstBI TTCGAA 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 1093, 1315
BstDEI CTNAG 6 cut(s) 101, 122, 177, 260, 759, 997
BstF5I GGATG 4 cut(s) 747, 965, 1033, 1070
BstFNI CGCG 3 cut(s) 747, 1234, 1311
BstHHI GCGC 3 cut(s) 747, 780, 1313
BstKTI GATC 1 cut(s) 870
BstMBI GATC 1 cut(s) 867
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 3 cut(s) 413, 902, 1199
BstNI CCWGG 2 cut(s) 703, 842
BstSCI CCNGG 3 cut(s) 701, 840, 1185
BstSFI CTRYAG 2 cut(s) 183, 424
BstSLI GKGCMC 1 cut(s) 1307
BstUI CGCG 3 cut(s) 747, 1234, 1311
BstXI CCANNNNNNTGG 2 cut(s) 497, 1065
BsuI GTATCC 1 cut(s) 848
BsuRI GGCC 4 cut(s) 167, 293, 1294, 1305
BtsCI GGATG 4 cut(s) 747, 965, 1033, 1070
BtsIMutI CAGTG 2 cut(s) 60, 1139
Cac8I GCNNGC 2 cut(s) 1093, 1315
CfoI GCGC 3 cut(s) 747, 780, 1313
Cfr13I GGNCC 5 cut(s) 166, 292, 1292, 1303, 1304
Csp6I GTAC 6 cut(s) 126, 252, 321, 370, 487, 542
CviAII CATG 5 cut(s) 356, 404, 556, 662, 1165
CviQI GTAC 6 cut(s) 126, 252, 321, 370, 487, 542
DdeI CTNAG 6 cut(s) 101, 122, 177, 260, 759, 997
DpnI GATC 1 cut(s) 869
DpnII GATC 1 cut(s) 867
DraIII CACNNNGTG 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 1295
Eco24I GRGCYC 1 cut(s) 1307
Eco57I CTGAAG 1 cut(s) 22
EcoO109I RGGNCCY 3 cut(s) 1292, 1303, 1304
EcoRI GAATTC 1 cut(s) 1223
EcoRII CCWGG 2 cut(s) 701, 840
EcoT14I CCWWGG 1 cut(s) 1295
EcoT38I GRGCYC 1 cut(s) 1307
ErhI CCWWGG 1 cut(s) 1295
FaeI CATG 5 cut(s) 359, 407, 559, 665, 1168
FalI AAGNNNNNCTT 2 cut(s) 153, 185
FatI CATG 5 cut(s) 355, 403, 555, 661, 1164
FauI CCCGC 1 cut(s) 1316
FauNDI CATATG 1 cut(s) 1014
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 1 cut(s) 789
FokI GGATG 4 cut(s) 754, 972, 1040, 1077
FriOI GRGCYC 1 cut(s) 1307
FspBI CTAG 2 cut(s) 1296, 1314
GlaI GCGC 3 cut(s) 746, 779, 1312
HaeIII GGCC 4 cut(s) 167, 293, 1294, 1305
HapII CCGG 1 cut(s) 1187
HhaI GCGC 3 cut(s) 747, 780, 1313
Hin1II CATG 5 cut(s) 359, 407, 559, 665, 1168
Hin6I GCGC 3 cut(s) 745, 778, 1311
HinP1I GCGC 3 cut(s) 745, 778, 1311
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 1089
HinfI GANTC 3 cut(s) 328, 472, 698
HpaI GTTAAC 1 cut(s) 51
HpaII CCGG 1 cut(s) 1187
HphI GGTGA 3 cut(s) 10, 803, 877
Hpy166II GTNNAC 4 cut(s) 51, 487, 790, 1268
Hpy188I TCNGA 2 cut(s) 102, 1222
Hpy188III TCNNGA 2 cut(s) 438, 683
Hpy8I GTNNAC 4 cut(s) 51, 487, 790, 1268
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 4 cut(s) 706, 886, 903, 1294
HpyCH4III ACNGT 5 cut(s) 25, 55, 64, 83, 541
HpyCH4IV ACGT 2 cut(s) 566, 1279
HpyCH4V TGCA 8 cut(s) 190, 257, 280, 365, 926, 1041, 1085, 1095
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 902, 1199
HpyF3I CTNAG 6 cut(s) 101, 122, 177, 260, 759, 997
HpySE526I ACGT 2 cut(s) 566, 1279
Hsp92II CATG 5 cut(s) 359, 407, 559, 665, 1168
HspAI GCGC 3 cut(s) 745, 778, 1311
Ksp22I TGATCA 1 cut(s) 867
KspAI GTTAAC 1 cut(s) 51
Kzo9I GATC 1 cut(s) 867
LmnI GCTCC 1 cut(s) 583
LweI GCATC 2 cut(s) 732, 733
MaeI CTAG 2 cut(s) 1296, 1314
MaeII ACGT 2 cut(s) 566, 1279
MalI GATC 1 cut(s) 869
MbiI CCGCTC 1 cut(s) 105
MboI GATC 1 cut(s) 867
MboII GAAGA 3 cut(s) 149, 655, 719
MfeI CAATTG 1 cut(s) 287
MhlI GDGCHC 2 cut(s) 1008, 1307
MluCI AATT 7 cut(s) 192, 228, 281, 287, 410, 1104, 1223
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 7 cut(s) 165, 353, 368, 659, 824, 1256, 1283
MseI TTAA 6 cut(s) 50, 203, 275, 920, 954, 1107
MslI CAYNNNNRTG 2 cut(s) 360, 1013
MspA1I CMGCKG 1 cut(s) 905
MspI CCGG 1 cut(s) 1187
MspR9I CCNGG 3 cut(s) 703, 842, 1187
MunI CAATTG 1 cut(s) 287
Mva1269I GAATGC 5 cut(s) 1041, 1077, 1193, 1242, 1253
MvaI CCWGG 2 cut(s) 703, 842
MvnI CGCG 3 cut(s) 747, 1234, 1311
MwoI GCNNNNNNNGC 3 cut(s) 413, 902, 1199
NciI CCSGG 1 cut(s) 1187
NdeI CATATG 1 cut(s) 1014
NdeII GATC 1 cut(s) 867
NheI GCTAGC 1 cut(s) 1313
NlaIII CATG 5 cut(s) 359, 407, 559, 665, 1168
NlaIV GGNNCC 5 cut(s) 248, 294, 1208, 1305, 1306
NspV TTCGAA 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 139
PctI GAATGC 5 cut(s) 1041, 1077, 1193, 1242, 1253
PfeI GAWTC 2 cut(s) 472, 698
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 1280
PshBI ATTAAT 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 701, 840
PspGI CCWGG 2 cut(s) 701, 840
PspN4I GGNNCC 5 cut(s) 248, 294, 1208, 1305, 1306
PspOMI GGGCCC 1 cut(s) 1303
PspPI GGNCC 5 cut(s) 166, 292, 1292, 1303, 1304
PvuII CAGCTG 1 cut(s) 905
RsaI GTAC 6 cut(s) 127, 253, 322, 371, 488, 543
RsaNI GTAC 6 cut(s) 126, 252, 321, 370, 487, 542
RseI CAYNNNNRTG 2 cut(s) 360, 1013
SaqAI TTAA 6 cut(s) 50, 203, 275, 920, 954, 1107
Sau3AI GATC 1 cut(s) 867
Sau96I GGNCC 5 cut(s) 166, 292, 1292, 1303, 1304
ScaI AGTACT 1 cut(s) 127
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 3 cut(s) 703, 842, 1187
SduI GDGCHC 2 cut(s) 1008, 1307
SfaNI GCATC 2 cut(s) 732, 733
SfcI CTRYAG 2 cut(s) 183, 424
SfuI TTCGAA 1 cut(s) 142
SmiMI CAYNNNNRTG 2 cut(s) 360, 1013
Sse9I AATT 7 cut(s) 192, 228, 281, 287, 410, 1104, 1223
SsiI CCGC 3 cut(s) 105, 747, 1309
SspMI CTAG 2 cut(s) 1296, 1314
StyD4I CCNGG 3 cut(s) 701, 840, 1185
StyI CCWWGG 1 cut(s) 1295
TaaI ACNGT 5 cut(s) 25, 55, 64, 83, 541
TaiI ACGT 2 cut(s) 569, 1282
TaqI TCGA 2 cut(s) 142, 946
TasI AATT 7 cut(s) 192, 228, 281, 287, 410, 1104, 1223
TatI WGTACW 3 cut(s) 125, 369, 541
TfiI GAWTC 2 cut(s) 472, 698
Tru1I TTAA 6 cut(s) 50, 203, 275, 920, 954, 1107
Tru9I TTAA 6 cut(s) 50, 203, 275, 920, 954, 1107
TscAI CASTG 2 cut(s) 60, 1146
TspDTI ATGAA 6 cut(s) 344, 392, 495, 544, 905, 1170
TspGWI ACGGA 2 cut(s) 239, 354
TspRI CASTG 2 cut(s) 60, 1146
VspI ATTAAT 1 cut(s) 275
XapI RAATTY 2 cut(s) 410, 1223
XmaJI CCTAGG 1 cut(s) 1295
XmiI GTMKAC 1 cut(s) 789
XspI CTAG 2 cut(s) 1296, 1314
ZrmI AGTACT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.