pycom17g13090

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006955
Physical Location & Seq
Forward (+)
10775456 .. 10777768
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g13090.1

Sequence Viewer

Length: 1479 bp
ATGAACCAAATATCACTTTTTGAGGCTAAGGTTTCAAATGGAAATGGAGAACAGACACTTAGCCGTGATGTTTACCGGCTTGGGCGTCGGTTCGATTTCTACAGAATGCTTTCCTTTTACTTCACAACAGTTGGATTCTATTTTAGTAGTTTGGTAACTGTGCTGACTGTATATGTGTTTTTGTATGGACGTATGTACCTGGTTATGAGTGGATTAGAGAATGAGATCCTGGAGAATCCAACCATACATGAGAACAAAGCCTTCGAAGAGGCTTTGGCTACCCAGTCTGTCTTTCAACTGGGCTTGTTGCTTGTGCTTCCAATGATCATGGAAATTGGCCTAGAGAAAGGGTTCCGCAGTGCCTTGGGTGATCTCATCATCATGCAGCTCCAGTTGGCCTCTGTTTTCTTCACATTCCAGCTGGGAACAAAAGCTCATTACTATGGAAGGACAATCTTACACGGAGGATCTAAATACCGAGCAACTGGCCGTGGTTTTGTTGTTTTCCATGCAAAGTTTTCCGAGAACTACAGACTTTACTCGCGCAGTCACTTTGTGAAAGGGTTGGAATTGCTTATTCTGCTGATTGTGTATGGAGTCTACGGGCAAGCGTATAGGAGTTCAAATCTTTATTTTTTCATCACTTTGTCCATGTGGTTTCTGGTTGCTTCCTGGTTGTTTGCTCCTTTCATATTCAATCCGTCCAGCTTTGACTGGCAGAAAACTGTAGATGATTGGACAGAATGGAAGAGGTGGATGGGAAATCGTGGTGGTATTGGGATAGCACCTGACAAAAGTTGGGAATCATGGTGGAATGAAGAACAGGAACACCTCAAATACACAGTTATCCGGGGAAGAATTCTTGAAATAATACTTGCGCTGCGGTTCCTTATTTACCAATATGGAATTGTTTACCACCTTGACATAGCCCATCACAGCAAGAGTTTACTGGTTTATGGACTTTCTTGGATAGTTATGGTAACTGTTCTTCTCGTTTTAAAGATGGTTTCCATGGGTAGACGAAGATTTGGCACCGACTTTCAGCTCATGTTCAGGATTCTGAAAGCGCTTTTGTTTCTTGGTTTCATGTCAGTCATGACTGTCCTATTTGTTGTTTGTGGCCTTACTATTTCAGATCTGTTTGCTGCTATCCTTGCCTTCCTACCCACAGGCTGGGCCCTTCTTCTGATCGGGCAAGCATGCAGGCCAATGTTCAAAGGGCTAGGATTCTGGGAATCGATCATGGAGCTTGGAAGAGCATACGATTACGTAATGGGGCTAGTAATCTTCATGCCGATAGCGATTTTGTCATGGTTCCCGTTTGTATCAGAGTTCCAAACACGTTTGCTCTTCAACCAAGCATTCAGCAGAGGCCTGCAGATTTCCATGATTCTTGCAGGGAGGAAAGACAAGACGGAGGGGTCTCGGACAGAGAGACCAGACAAGCCTTCAGCATCTCAAGTTCATACCCGCAATTAG

Protein Analysis

493

Amino Acids

56.86

Weight (kDa)

9.47

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucan_synthase PF02364 1 - 288 3.8e-76 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1420
AccB1I GGYRCC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 1173
AccI GTMKAC 2 cut(s) 600, 1018
AccII CGCG 1 cut(s) 544
AciI CCGC 3 cut(s) 355, 883, 1471
AclWI GGATC 2 cut(s) 220, 475
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 1 cut(s) 858
AcuI CTGAAG 1 cut(s) 1434
AcyI GRCGYC 1 cut(s) 85
AdeI CACNNNGTG 1 cut(s) 556
AfaI GTAC 1 cut(s) 197
AfeI AGCGCT 1 cut(s) 1068
AfiI CCNNNNNNNGG 1 cut(s) 1173
AflIII ACRYGT 1 cut(s) 1340
AgsI TTSAA 7 cut(s) 36, 296, 624, 697, 866, 1216, 1354
AjnI CCWGG 3 cut(s) 198, 228, 671
AluBI AGCT 6 cut(s) 388, 421, 434, 708, 1045, 1249
AluI AGCT 6 cut(s) 388, 421, 434, 708, 1045, 1249
Alw26I GTCTC 2 cut(s) 1428, 1429
AlwI GGATC 2 cut(s) 220, 475
Aor51HI AGCGCT 1 cut(s) 1068
AoxI GGCC 7 cut(s) 337, 396, 487, 1120, 1176, 1205, 1372
ApaI GGGCCC 1 cut(s) 1180
ApeKI GCWGC 3 cut(s) 385, 880, 1145
ApoI RAATTY 1 cut(s) 858
Asp700I GAANNNNTTC 2 cut(s) 109, 350
AspLEI GCGC 3 cut(s) 546, 880, 1069
AspS9I GGNCC 2 cut(s) 1176, 1177
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 1 cut(s) 380
AsuII TTCGAA 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 1180
BaeI ACNNNNGTAYC 2 cut(s) 179, 212
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 1 cut(s) 1180
BarI GAAGNNNNNNTAC 2 cut(s) 972, 1004
BbvI GCAGC 3 cut(s) 397, 867, 1132
BccI CCATC 3 cut(s) 751, 939, 997
BceAI ACGGC 2 cut(s) 48, 474
BciT130I CCWGG 3 cut(s) 200, 230, 673
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 2 cut(s) 1428, 1429
BfaI CTAG 3 cut(s) 341, 1223, 1280
BfmI CTRYAG 4 cut(s) 100, 529, 726, 1376
BfoI RGCGCY 1 cut(s) 1070
BglII AGATCT 1 cut(s) 1135
BisI GCNGC 3 cut(s) 386, 881, 1146
BlsI GCNGC 3 cut(s) 387, 882, 1147
Bme1390I CCNGG 4 cut(s) 200, 230, 673, 851
BmgT120I GGNCC 2 cut(s) 1176, 1177
BmiI GGNNCC 5 cut(s) 353, 887, 1033, 1178, 1316
BmrFI CCNGG 4 cut(s) 200, 230, 673, 851
BmrI ACTGGG 2 cut(s) 277, 308
BmsI GCATC 1 cut(s) 1463
BmuI ACTGGG 2 cut(s) 277, 308
BpmI CTGGAG 2 cut(s) 251, 374
Bpu10I CCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 1443
BpuMI CCSGG 1 cut(s) 851
Bsa29I ATCGAT 1 cut(s) 1238
BsaAI YACGTR 1 cut(s) 1270
BsaHI GRCGYC 1 cut(s) 85
BsaI GGTCTC 2 cut(s) 1428, 1429
BsaJI CCNNGG 4 cut(s) 363, 490, 850, 1011
Bsc4I CCNNNNNNNGG 1 cut(s) 1173
Bse118I RCCGGY 1 cut(s) 75
Bse1I ACTGG 6 cut(s) 283, 303, 391, 490, 719, 954
BseBI CCWGG 3 cut(s) 200, 230, 673
BseCI ATCGAT 1 cut(s) 1238
BseDI CCNNGG 4 cut(s) 363, 490, 850, 1011
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 1 cut(s) 1173
BseNI ACTGG 6 cut(s) 283, 303, 391, 490, 719, 954
BseSI GKGCMC 1 cut(s) 1180
BseXI GCAGC 3 cut(s) 397, 867, 1132
BseYI CCCAGC 2 cut(s) 421, 1173
Bsh1236I CGCG 1 cut(s) 544
BshFI GGCC 7 cut(s) 339, 398, 489, 1122, 1178, 1207, 1374
BshNI GGYRCC 1 cut(s) 1031
BshVI ATCGAT 1 cut(s) 1238
BsiSI CCGG 2 cut(s) 76, 850
BslI CCNNNNNNNGG 1 cut(s) 1173
BsmAI GTCTC 2 cut(s) 1428, 1429
BsmI GAATGC 2 cut(s) 111, 1361
BsnI GGCC 7 cut(s) 339, 398, 489, 1122, 1178, 1207, 1374
Bso31I GGTCTC 2 cut(s) 1428, 1429
Bsp119I TTCGAA 1 cut(s) 264
Bsp120I GGGCCC 1 cut(s) 1176
Bsp1286I GDGCHC 1 cut(s) 1180
Bsp143I GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
Bsp19I CCATGG 1 cut(s) 1011
BspACI CCGC 3 cut(s) 355, 883, 1471
BspANI GGCC 7 cut(s) 339, 398, 489, 1122, 1178, 1207, 1374
BspDI ATCGAT 1 cut(s) 1238
BspFNI CGCG 1 cut(s) 544
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 5 cut(s) 353, 887, 1033, 1178, 1316
BspMAI CTGCAG 1 cut(s) 1380
BspPI GGATC 2 cut(s) 220, 475
BspQI GCTCTTC 2 cut(s) 1249, 1355
BspT104I TTCGAA 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 1031
BspTNI GGTCTC 2 cut(s) 1428, 1429
BsrFI RCCGGY 1 cut(s) 75
BsrI ACTGG 6 cut(s) 283, 303, 391, 490, 719, 954
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 4 cut(s) 363, 490, 850, 1011
BssMI GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
BssNI GRCGYC 1 cut(s) 85
BssT1I CCWWGG 2 cut(s) 363, 1011
Bst2UI CCWGG 3 cut(s) 200, 230, 673
Bst4CI ACNGT 7 cut(s) 130, 160, 169, 727, 844, 985, 1102
Bst6I CTCTTC 4 cut(s) 261, 743, 1249, 1355
BstACI GRCGYC 1 cut(s) 85
BstBAI YACGTR 1 cut(s) 1270
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 5 cut(s) 609, 1197, 1201, 1205, 1376
BstDEI CTNAG 2 cut(s) 27, 59
BstDSI CCRYGG 2 cut(s) 490, 1011
BstF5I GGATG 1 cut(s) 762
BstFNI CGCG 1 cut(s) 544
BstH2I RGCGCY 1 cut(s) 1070
BstHHI GCGC 3 cut(s) 546, 880, 1069
BstKTI GATC 7 cut(s) 228, 327, 373, 470, 1138, 1191, 1242
BstMAI GTCTC 2 cut(s) 1428, 1429
BstMBI GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
BstMWI GCNNNNNNNGC 2 cut(s) 580, 1154
BstNI CCWGG 3 cut(s) 200, 230, 673
BstNSI RCATGY 1 cut(s) 1203
BstSCI CCNGG 4 cut(s) 198, 228, 671, 849
BstSFI CTRYAG 4 cut(s) 100, 529, 726, 1376
BstSLI GKGCMC 1 cut(s) 1180
BstSNI TACGTA 1 cut(s) 1270
BstUI CGCG 1 cut(s) 544
BstV1I GCAGC 3 cut(s) 397, 867, 1132
BstX2I RGATCY 3 cut(s) 225, 467, 1135
BstYI RGATCY 3 cut(s) 225, 467, 1135
Bsu15I ATCGAT 1 cut(s) 1238
BsuRI GGCC 7 cut(s) 339, 398, 489, 1122, 1178, 1207, 1374
BsuTUI ATCGAT 1 cut(s) 1238
BtgI CCRYGG 2 cut(s) 490, 1011
BtsCI GGATG 1 cut(s) 762
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 5 cut(s) 609, 1197, 1201, 1205, 1376
CciI TCATGA 1 cut(s) 1095
CfoI GCGC 3 cut(s) 546, 880, 1069
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 1176, 1177
ClaI ATCGAT 1 cut(s) 1238
CseI GACGC 1 cut(s) 74
CsiI ACCWGGT 1 cut(s) 198
Csp6I GTAC 1 cut(s) 196
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 27, 59
DpnI GATC 7 cut(s) 227, 326, 372, 469, 1137, 1190, 1241
DpnII GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
DraI TTTAAA 1 cut(s) 999
DraIII CACNNNGTG 1 cut(s) 556
DrdI GACNNNNNNGTC 1 cut(s) 1420
DseDI GACNNNNNNGTC 1 cut(s) 1420
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 4 cut(s) 261, 743, 1249, 1355
EarI CTCTTC 4 cut(s) 261, 743, 1249, 1355
Eco105I TACGTA 1 cut(s) 1270
Eco130I CCWWGG 2 cut(s) 363, 1011
Eco147I AGGCCT 1 cut(s) 1374
Eco24I GRGCYC 1 cut(s) 1180
Eco31I GGTCTC 2 cut(s) 1428, 1429
Eco47III AGCGCT 1 cut(s) 1068
Eco57I CTGAAG 1 cut(s) 1434
EcoO109I RGGNCCY 1 cut(s) 1177
EcoRI GAATTC 1 cut(s) 858
EcoRII CCWGG 3 cut(s) 198, 228, 671
EcoT14I CCWWGG 2 cut(s) 363, 1011
EcoT38I GRGCYC 1 cut(s) 1180
ErhI CCWWGG 2 cut(s) 363, 1011
FbaI TGATCA 1 cut(s) 324
FblI GTMKAC 2 cut(s) 600, 1018
Fnu4HI GCNGC 3 cut(s) 386, 881, 1146
FokI GGATG 1 cut(s) 769
FriOI GRGCYC 1 cut(s) 1180
Fsp4HI GCNGC 3 cut(s) 386, 881, 1146
FspBI CTAG 3 cut(s) 341, 1223, 1280
GlaI GCGC 3 cut(s) 545, 879, 1068
GluI GCNGC 3 cut(s) 386, 881, 1146
GsaI CCCAGC 2 cut(s) 425, 1177
GsuI CTGGAG 2 cut(s) 251, 374
HaeII RGCGCY 1 cut(s) 1070
HaeIII GGCC 7 cut(s) 339, 398, 489, 1122, 1178, 1207, 1374
HapII CCGG 2 cut(s) 76, 850
HgaI GACGC 1 cut(s) 74
HhaI GCGC 3 cut(s) 546, 880, 1069
Hin1I GRCGYC 1 cut(s) 85
Hin6I GCGC 3 cut(s) 544, 878, 1067
HinP1I GCGC 3 cut(s) 544, 878, 1067
HinfI GANTC 8 cut(s) 135, 235, 597, 803, 1057, 1227, 1235, 1390
HpaII CCGG 2 cut(s) 76, 850
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 5 cut(s) 73, 601, 913, 947, 1019
Hpy188I TCNGA 6 cut(s) 523, 1062, 1135, 1188, 1330, 1428
Hpy188III TCNNGA 3 cut(s) 863, 1054, 1096
Hpy8I GTNNAC 5 cut(s) 73, 601, 913, 947, 1019
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 5 cut(s) 271, 441, 1168, 1190, 1458
HpyCH4III ACNGT 7 cut(s) 130, 160, 169, 727, 844, 985, 1102
HpyCH4IV ACGT 3 cut(s) 190, 1269, 1342
HpyCH4V TGCA 5 cut(s) 385, 512, 1203, 1378, 1397
HpyF10VI GCNNNNNNNGC 2 cut(s) 580, 1154
HpyF3I CTNAG 2 cut(s) 27, 59
HpySE526I ACGT 3 cut(s) 190, 1269, 1342
Hsp92I GRCGYC 1 cut(s) 85
HspAI GCGC 3 cut(s) 544, 878, 1067
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
LguI GCTCTTC 2 cut(s) 1249, 1355
LmnI GCTCC 3 cut(s) 393, 688, 1246
Lsp1109I GCAGC 3 cut(s) 397, 867, 1132
LweI GCATC 1 cut(s) 1463
MabI ACCWGGT 1 cut(s) 198
MaeI CTAG 3 cut(s) 341, 1223, 1280
MaeII ACGT 3 cut(s) 190, 1269, 1342
MaeIII GTNAC 3 cut(s) 154, 548, 979
MalI GATC 7 cut(s) 227, 326, 372, 469, 1137, 1190, 1241
MboI GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
MflI RGATCY 3 cut(s) 225, 467, 1135
MhlI GDGCHC 1 cut(s) 1180
MluCI AATT 5 cut(s) 333, 569, 858, 906, 1474
MlyI GAGTC 1 cut(s) 606
MmeI TCCRAC 3 cut(s) 112, 263, 546
MnlI CCTC 9 cut(s) 16, 262, 409, 458, 744, 842, 1364, 1395, 1411
MroXI GAANNNNTTC 2 cut(s) 109, 350
MseI TTAA 1 cut(s) 998
MslI CAYNNNNRTG 2 cut(s) 380, 441
MspA1I CMGCKG 1 cut(s) 421
MspI CCGG 2 cut(s) 76, 850
MspR9I CCNGG 4 cut(s) 200, 230, 673, 851
Mva1269I GAATGC 2 cut(s) 111, 1361
MvaI CCWGG 3 cut(s) 200, 230, 673
MvnI CGCG 1 cut(s) 544
MwoI GCNNNNNNNGC 2 cut(s) 580, 1154
NciI CCSGG 1 cut(s) 851
NcoI CCATGG 1 cut(s) 1011
NdeII GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
NlaIV GGNNCC 5 cut(s) 353, 887, 1033, 1178, 1316
NmuCI GTSAC 1 cut(s) 548
NspI RCATGY 1 cut(s) 1203
NspV TTCGAA 1 cut(s) 264
PaeI GCATGC 1 cut(s) 1203
PagI TCATGA 1 cut(s) 1095
PceI AGGCCT 1 cut(s) 1374
PciSI GCTCTTC 2 cut(s) 1249, 1355
PctI GAATGC 2 cut(s) 111, 1361
PdmI GAANNNNTTC 2 cut(s) 109, 350
PfeI GAWTC 7 cut(s) 135, 235, 803, 1057, 1227, 1235, 1390
PflMI CCANNNNNTGG 1 cut(s) 1173
PfoI TCCNGGA 1 cut(s) 228
PkrI GCNGC 3 cut(s) 387, 882, 1147
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
Ppu21I YACGTR 1 cut(s) 1270
Psp6I CCWGG 3 cut(s) 198, 228, 671
PspFI CCCAGC 2 cut(s) 421, 1173
PspGI CCWGG 3 cut(s) 198, 228, 671
PspN4I GGNNCC 5 cut(s) 353, 887, 1033, 1178, 1316
PspOMI GGGCCC 1 cut(s) 1176
PspPI GGNCC 2 cut(s) 1176, 1177
PstI CTGCAG 1 cut(s) 1380
PsuI RGATCY 3 cut(s) 225, 467, 1135
PvuII CAGCTG 1 cut(s) 421
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 2 cut(s) 380, 441
SapI GCTCTTC 2 cut(s) 1249, 1355
SaqAI TTAA 1 cut(s) 998
SatI GCNGC 3 cut(s) 386, 881, 1146
Sau3AI GATC 7 cut(s) 225, 324, 370, 467, 1135, 1188, 1239
Sau96I GGNCC 2 cut(s) 1176, 1177
SchI GAGTC 1 cut(s) 606
ScrFI CCNGG 4 cut(s) 200, 230, 673, 851
SduI GDGCHC 1 cut(s) 1180
SexAI ACCWGGT 1 cut(s) 198
SfaNI GCATC 1 cut(s) 1463
SfcI CTRYAG 4 cut(s) 100, 529, 726, 1376
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 380, 441
SmlI CTYRAG 1 cut(s) 1458
SmoI CTYRAG 1 cut(s) 1458
SnaBI TACGTA 1 cut(s) 1270
SphI GCATGC 1 cut(s) 1203
Sse9I AATT 5 cut(s) 333, 569, 858, 906, 1474
SseBI AGGCCT 1 cut(s) 1374
SsiI CCGC 3 cut(s) 355, 883, 1471
SspMI CTAG 3 cut(s) 341, 1223, 1280
StuI AGGCCT 1 cut(s) 1374
StyD4I CCNGG 4 cut(s) 198, 228, 671, 849
StyI CCWWGG 2 cut(s) 363, 1011
TaaI ACNGT 7 cut(s) 130, 160, 169, 727, 844, 985, 1102
TaiI ACGT 3 cut(s) 193, 1272, 1345
TaqI TCGA 3 cut(s) 93, 264, 1238
TasI AATT 5 cut(s) 333, 569, 858, 906, 1474
TfiI GAWTC 7 cut(s) 135, 235, 803, 1057, 1227, 1235, 1390
Tru1I TTAA 1 cut(s) 998
Tru9I TTAA 1 cut(s) 998
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 3 cut(s) 385, 880, 1145
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 7 cut(s) 17, 628, 679, 831, 1075, 1279, 1454
TspGWI ACGGA 3 cut(s) 477, 690, 1430
TspRI CASTG 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 1173
XapI RAATTY 1 cut(s) 858
XceI RCATGY 1 cut(s) 1203
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XmiI GTMKAC 2 cut(s) 600, 1018
XmnI GAANNNNTTC 2 cut(s) 109, 350
XspI CTAG 3 cut(s) 341, 1223, 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.