MD07G1008000.v1.1

Anthocyanin 5-aromatic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005947
Physical Location & Seq
Forward (+)
733538 .. 734812
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1008000.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGACTTCCCATTTCACTTCCACCACCCTCCCAAACCTCAAGCACTCTCTCTCCCTCGCTCTCCAACAATTTTACCCTTTCGCCGGAACCCTACTGGCCCGGCCTCATCCCGAAAAGCCCGAGCTTCTCTACACGCAAGGCGACTCGGTGTCATTGACTGTCGTGGAGTCGGACGGGGACTTTGAGTATCTCTCGAGCAATTCCCAAAGAGGTGCGGTAGAGTTTCACACACTTGTGCCGGAGCTAGCGTCGACAGGTAGTATAGCACATTGTAGTAATGAGGCACAAGCAGCAGATATTTCTTTGTTAAGTGTTCAAATTACAGTGTTCCCAAATTGTGGGATTTGCATTGGGATTGCTTACCACCACGTTGTTGGTGATGAGAGGACCTTTAACAATTTCATCAAGGCTTGGGCCTCATTTTCTCGTCTCGGAGACTCGTCAAACATAGCCGTACAATCAGCGCCCTACTATGATCGTACGGCTATTGTCGATGCACTTGGTCTCGAGGATATCTTCTTGAAGGAGTGGTGGAAGCGAATTTCTTCACAAGGAGTGGTTCTAGGTGCTAATAAGAATGCCATTGTTAATGGGCATTCGAAAAACATGATTAGGGCAACATTTTTGATTGACTTGGCCCAAATGGAGAGCATCAAGAATTGGATTATTGAGCTATGCAACAAGGGAAATGAGTCGCATCCGGTACATTTATCACCATATGTCCTAACGTGTGCATTTACATGGGTTTGTTTGCTTAAAACCCAAGAAAATGATAACAACTACAGCAAGTGTTGCGATGAAGATCCAAATTATTTTGGGTTTATTGCGGGCGGGATAACTCGTTTGGGTCATCCAGTACCAGCTACGTATGTCGGTAATTGTGTTGGATTTGGCCGGTCAGTGGCGTCAACGAAAGCGCTACTAGGGGCAAATGGAGTTGTTGTTGCGGCGAAGGCAATCGGAAACACTATTAAGAAGTTAGACAAGGCCATTTTGGGAGGGGCAGAGAAGTGGATTTCGGAGTGGGAGGAGATGTTCGGGTCGGAGCTCCACGTCATGGTTCTCGGGTCACCGAAGGTGGATCTGTATGAGATAGATTTTGGATGGGGCAGGCCTAGAAAGATAGAGGAGATTGGAATTGATGGTGTGACAGGCATTTCACTGAGTGAGAGTAGAGACGTGTCGGGTGGCATTGAGGTTGGCTTGGTCCTACCAAAGCCTCGAATGGATGCTTTCACAACTTTAATCATCGACGGACTCAATGCCATTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

425

Amino Acids

46.1

Weight (kDa)

5.48

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 413 3.7e-30 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 338, 1057
AccI GTMKAC 1 cut(s) 251
AciI CCGC 4 cut(s) 215, 827, 831, 947
AclWI GGATC 2 cut(s) 797, 1089
AcoI YGGCCR 1 cut(s) 892
AcsI RAATTY 1 cut(s) 540
AcyI GRCGYC 1 cut(s) 905
AdeI CACNNNGTG 1 cut(s) 1166
AfaI GTAC 4 cut(s) 456, 481, 705, 858
AfeI AGCGCT 1 cut(s) 918
AfiI CCNNNNNNNGG 4 cut(s) 83, 338, 901, 1057
AflIII ACRYGT 2 cut(s) 728, 1179
AgsI TTSAA 2 cut(s) 317, 523
AhdI GACNNNNNGTC 1 cut(s) 148
AjiI CACGTC 2 cut(s) 1054, 1180
AleI CACNNNNGTG 1 cut(s) 233
AloI GAACNNNNNNTCC 2 cut(s) 1019, 1051
AluBI AGCT 5 cut(s) 124, 244, 673, 863, 1048
AluI AGCT 5 cut(s) 124, 244, 673, 863, 1048
Alw21I GWGCWC 1 cut(s) 1050
Alw26I GTCTC 4 cut(s) 429, 434, 509, 1170
AlwI GGATC 2 cut(s) 797, 1089
Ama87I CYCGRG 4 cut(s) 119, 193, 506, 1064
Aor51HI AGCGCT 1 cut(s) 918
AoxI GGCC 7 cut(s) 96, 101, 414, 636, 892, 987, 1112
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 540
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
Asp700I GAANNNNTTC 1 cut(s) 544
AspLEI GCGC 2 cut(s) 466, 919
AspS9I GGNCC 5 cut(s) 97, 387, 414, 637, 1207
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 389, 705, 1062
AsuII TTCGAA 1 cut(s) 599
AsuNHI GCTAGC 1 cut(s) 244
AvaI CYCGRG 4 cut(s) 119, 193, 506, 1064
AvaII GGWCC 2 cut(s) 387, 1207
BanII GRGCYC 1 cut(s) 1050
Bbv12I GWGCWC 1 cut(s) 1050
BbvI GCAGC 1 cut(s) 302
BccI CCATC 2 cut(s) 1098, 1136
BceAI ACGGC 2 cut(s) 437, 498
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 4 cut(s) 429, 434, 509, 1170
BfaI CTAG 4 cut(s) 245, 563, 923, 1116
BfmI CTRYAG 1 cut(s) 781
BfoI RGCGCY 2 cut(s) 467, 920
BisI GCNGC 2 cut(s) 291, 948
BlsI GCNGC 2 cut(s) 292, 949
Bme1390I CCNGG 1 cut(s) 100
Bme18I GGWCC 2 cut(s) 387, 1207
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 4 cut(s) 119, 193, 506, 1064
BmgBI CACGTC 2 cut(s) 1054, 1180
BmgT120I GGNCC 5 cut(s) 97, 387, 414, 637, 1207
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 100
BmsI GCATC 4 cut(s) 484, 660, 706, 1219
BmtI GCTAGC 1 cut(s) 248
BplI GAGNNNNNCTC 2 cut(s) 176, 208
Bpu14I TTCGAA 1 cut(s) 599
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 867
BsaBI GATNNNNATC 1 cut(s) 801
BsaHI GRCGYC 1 cut(s) 905
BsaI GGTCTC 1 cut(s) 509
BsaWI WCCGGW 1 cut(s) 700
BsaXI ACNNNNNCTCC 8 cut(s) 35, 65, 233, 263, 927, 957, 1019, 1049
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 338, 901, 1057
Bse118I RCCGGY 1 cut(s) 894
Bse1I ACTGG 2 cut(s) 99, 854
Bse8I GATNNNNATC 1 cut(s) 801
BseGI GGATG 5 cut(s) 106, 697, 850, 1109, 1234
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 4 cut(s) 83, 338, 901, 1057
BseMII CTCAG 1 cut(s) 1154
BseNI ACTGG 2 cut(s) 99, 854
BseRI GAGGAG 2 cut(s) 1043, 1142
BseXI GCAGC 1 cut(s) 302
BshFI GGCC 7 cut(s) 98, 103, 416, 638, 894, 989, 1114
BsiHKAI GWGCWC 1 cut(s) 1050
BsiHKCI CYCGRG 4 cut(s) 119, 193, 506, 1064
BsiSI CCGG 5 cut(s) 84, 100, 239, 701, 895
BsiWI CGTACG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 4 cut(s) 83, 338, 901, 1057
BsmAI GTCTC 4 cut(s) 429, 434, 509, 1170
BsmBI CGTCTC 2 cut(s) 434, 1170
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 2 cut(s) 583, 595
BsnI GGCC 7 cut(s) 98, 103, 416, 638, 894, 989, 1114
Bso31I GGTCTC 1 cut(s) 509
BsoBI CYCGRG 4 cut(s) 119, 193, 506, 1064
Bsp119I TTCGAA 1 cut(s) 599
Bsp1286I GDGCHC 1 cut(s) 1050
Bsp143I GATC 3 cut(s) 475, 802, 1081
BspACI CCGC 4 cut(s) 215, 827, 831, 947
BspANI GGCC 7 cut(s) 98, 103, 416, 638, 894, 989, 1114
BspCNI CTCAG 1 cut(s) 1155
BspHI TCATGA 1 cut(s) 1271
BspLI GGNNCC 1 cut(s) 88
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 797, 1089
BspT104I TTCGAA 1 cut(s) 599
BspTNI GGTCTC 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 894
BsrI ACTGG 2 cut(s) 99, 854
BssAI RCCGGY 1 cut(s) 894
BssMI GATC 3 cut(s) 475, 802, 1081
BssNI GRCGYC 1 cut(s) 905
Bst4CI ACNGT 2 cut(s) 160, 325
BstACI GRCGYC 1 cut(s) 905
BstAPI GCANNNNNTGC 1 cut(s) 792
BstBAI YACGTR 1 cut(s) 867
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 3 cut(s) 246, 829, 1112
BstDEI CTNAG 1 cut(s) 1163
BstEII GGTNACC 1 cut(s) 1068
BstF5I GGATG 5 cut(s) 106, 697, 850, 1109, 1234
BstH2I RGCGCY 2 cut(s) 467, 920
BstHHI GCGC 2 cut(s) 466, 919
BstKTI GATC 3 cut(s) 478, 805, 1084
BstMAI GTCTC 4 cut(s) 429, 434, 509, 1170
BstMBI GATC 3 cut(s) 475, 802, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 290, 792, 953
BstPI GGTNACC 1 cut(s) 1068
BstSCI CCNGG 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 781
BstSNI TACGTA 1 cut(s) 867
BstV1I GCAGC 1 cut(s) 302
BstX2I RGATCY 2 cut(s) 802, 1081
BstXI CCANNNNNNTGG 1 cut(s) 374
BstYI RGATCY 2 cut(s) 802, 1081
BsuRI GGCC 7 cut(s) 98, 103, 416, 638, 894, 989, 1114
BtgZI GCGATG 1 cut(s) 810
BtrI CACGTC 2 cut(s) 1054, 1180
BtsCI GGATG 5 cut(s) 106, 697, 850, 1109, 1234
BtsIMutI CAGTG 3 cut(s) 330, 906, 1160
Cac8I GCNNGC 3 cut(s) 246, 829, 1112
CciI TCATGA 1 cut(s) 1271
CfoI GCGC 2 cut(s) 466, 919
Cfr10I RCCGGY 1 cut(s) 894
Cfr13I GGNCC 5 cut(s) 97, 387, 414, 637, 1207
CseI GACGC 2 cut(s) 237, 894
Csp6I GTAC 4 cut(s) 455, 480, 704, 857
CviAII CATG 4 cut(s) 607, 741, 1057, 1272
CviQI GTAC 4 cut(s) 455, 480, 704, 857
DdeI CTNAG 1 cut(s) 1163
DpnI GATC 3 cut(s) 477, 804, 1083
DpnII GATC 3 cut(s) 475, 802, 1081
DraIII CACNNNGTG 1 cut(s) 1166
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 892
Eam1105I GACNNNNNGTC 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 1048
Eco105I TACGTA 1 cut(s) 867
Eco147I AGGCCT 1 cut(s) 1114
Eco24I GRGCYC 1 cut(s) 1050
Eco31I GGTCTC 1 cut(s) 509
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 2 cut(s) 387, 1207
Eco47III AGCGCT 1 cut(s) 918
Eco53kI GAGCTC 1 cut(s) 1048
Eco88I CYCGRG 4 cut(s) 119, 193, 506, 1064
Eco91I GGTNACC 1 cut(s) 1068
EcoICRI GAGCTC 1 cut(s) 1048
EcoO109I RGGNCCY 1 cut(s) 387
EcoO65I GGTNACC 1 cut(s) 1068
EcoRV GATATC 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 1050
Esp3I CGTCTC 2 cut(s) 434, 1170
FaeI CATG 4 cut(s) 610, 744, 1060, 1275
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 606, 740, 1056, 1271
FauI CCCGC 2 cut(s) 820, 824
FauNDI CATATG 1 cut(s) 718
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 2 cut(s) 291, 948
FokI GGATG 5 cut(s) 93, 684, 837, 1116, 1241
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 2 cut(s) 291, 948
FspBI CTAG 4 cut(s) 245, 563, 923, 1116
GlaI GCGC 2 cut(s) 465, 918
GluI GCNGC 2 cut(s) 291, 948
HaeII RGCGCY 2 cut(s) 467, 920
HaeIII GGCC 7 cut(s) 98, 103, 416, 638, 894, 989, 1114
HapII CCGG 5 cut(s) 84, 100, 239, 701, 895
HgaI GACGC 2 cut(s) 237, 894
HhaI GCGC 2 cut(s) 466, 919
Hin1I GRCGYC 1 cut(s) 905
Hin1II CATG 4 cut(s) 610, 744, 1060, 1275
Hin6I GCGC 2 cut(s) 464, 917
HinP1I GCGC 2 cut(s) 464, 917
HincII GTYRAC 2 cut(s) 252, 909
HindII GTYRAC 2 cut(s) 252, 909
HinfI GANTC 5 cut(s) 143, 167, 437, 692, 1257
HpaII CCGG 5 cut(s) 84, 100, 239, 701, 895
HphI GGTGA 3 cut(s) 389, 705, 1062
Hpy166II GTNNAC 2 cut(s) 252, 909
Hpy188I TCNGA 5 cut(s) 172, 434, 962, 1021, 1045
Hpy188III TCNNGA 6 cut(s) 110, 193, 506, 520, 655, 1272
Hpy8I GTNNAC 2 cut(s) 252, 909
Hpy99I CGWCG 2 cut(s) 253, 1256
HpyAV CCTTC 3 cut(s) 517, 946, 1069
HpyCH4III ACNGT 2 cut(s) 160, 325
HpyCH4IV ACGT 5 cut(s) 369, 728, 866, 1053, 1179
HpyCH4V TGCA 4 cut(s) 348, 497, 678, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 792, 953
HpyF3I CTNAG 1 cut(s) 1163
HpySE526I ACGT 5 cut(s) 369, 728, 866, 1053, 1179
Hsp92I GRCGYC 1 cut(s) 905
Hsp92II CATG 4 cut(s) 610, 744, 1060, 1275
HspAI GCGC 2 cut(s) 464, 917
Kzo9I GATC 3 cut(s) 475, 802, 1081
LmnI GCTCC 3 cut(s) 241, 1045, 1053
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 4 cut(s) 484, 660, 706, 1219
MaeI CTAG 4 cut(s) 245, 563, 923, 1116
MaeII ACGT 5 cut(s) 369, 728, 866, 1053, 1179
MaeIII GTNAC 2 cut(s) 1068, 1147
MalI GATC 3 cut(s) 477, 804, 1083
MboI GATC 3 cut(s) 475, 802, 1081
MboII GAAGA 3 cut(s) 508, 537, 812
MflI RGATCY 2 cut(s) 802, 1081
MhlI GDGCHC 1 cut(s) 1050
MlyI GAGTC 5 cut(s) 137, 176, 431, 701, 1251
MmeI TCCRAC 4 cut(s) 88, 150, 865, 1023
MroXI GAANNNNTTC 1 cut(s) 544
MseI TTAA 6 cut(s) 308, 393, 588, 756, 972, 1244
MslI CAYNNNNRTG 2 cut(s) 233, 739
MspI CCGG 5 cut(s) 84, 100, 239, 701, 895
MspR9I CCNGG 1 cut(s) 100
Mva1269I GAATGC 2 cut(s) 583, 595
MwoI GCNNNNNNNGC 3 cut(s) 290, 792, 953
NciI CCSGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 718
NdeII GATC 3 cut(s) 475, 802, 1081
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 4 cut(s) 610, 744, 1060, 1275
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 1068, 1147
NspV TTCGAA 1 cut(s) 599
OliI CACNNNNGTG 1 cut(s) 233
PaeR7I CTCGAG 2 cut(s) 193, 506
PagI TCATGA 1 cut(s) 1271
PceI AGGCCT 1 cut(s) 1114
PctI GAATGC 2 cut(s) 583, 595
PdmI GAANNNNTTC 1 cut(s) 544
Pfl23II CGTACG 1 cut(s) 479
PflMI CCANNNNNTGG 2 cut(s) 338, 1057
PkrI GCNGC 2 cut(s) 292, 949
PleI GAGTC 5 cut(s) 137, 175, 431, 700, 1251
PpsI GAGTC 5 cut(s) 137, 175, 431, 700, 1251
Ppu21I YACGTR 1 cut(s) 867
PpuMI RGGWCCY 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 1050
Psp5II RGGWCCY 1 cut(s) 387
PspEI GGTNACC 1 cut(s) 1068
PspLI CGTACG 1 cut(s) 479
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 5 cut(s) 97, 387, 414, 637, 1207
PspPPI RGGWCCY 1 cut(s) 387
PsuI RGATCY 2 cut(s) 802, 1081
RsaI GTAC 4 cut(s) 456, 481, 705, 858
RsaNI GTAC 4 cut(s) 455, 480, 704, 857
RseI CAYNNNNRTG 2 cut(s) 233, 739
SacI GAGCTC 1 cut(s) 1050
SalI GTCGAC 1 cut(s) 250
SaqAI TTAA 6 cut(s) 308, 393, 588, 756, 972, 1244
SatI GCNGC 2 cut(s) 291, 948
Sau3AI GATC 3 cut(s) 475, 802, 1081
Sau96I GGNCC 5 cut(s) 97, 387, 414, 637, 1207
SchI GAGTC 5 cut(s) 137, 176, 431, 701, 1251
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 1 cut(s) 1050
SfaNI GCATC 4 cut(s) 484, 660, 706, 1219
SfcI CTRYAG 1 cut(s) 781
Sfr274I CTCGAG 2 cut(s) 193, 506
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 2 cut(s) 387, 1207
SlaI CTCGAG 2 cut(s) 193, 506
SmiMI CAYNNNNRTG 2 cut(s) 233, 739
SmlI CTYRAG 3 cut(s) 38, 193, 506
SmoI CTYRAG 3 cut(s) 38, 193, 506
SnaBI TACGTA 1 cut(s) 867
SseBI AGGCCT 1 cut(s) 1114
SsiI CCGC 4 cut(s) 215, 827, 831, 947
SspMI CTAG 4 cut(s) 245, 563, 923, 1116
SstI GAGCTC 1 cut(s) 1050
StuI AGGCCT 1 cut(s) 1114
StyD4I CCNGG 1 cut(s) 98
TaaI ACNGT 2 cut(s) 160, 325
TaiI ACGT 5 cut(s) 372, 731, 869, 1056, 1182
TaqI TCGA 7 cut(s) 194, 251, 492, 507, 599, 1222, 1251
TauI GCSGC 1 cut(s) 950
Tru1I TTAA 6 cut(s) 308, 393, 588, 756, 972, 1244
Tru9I TTAA 6 cut(s) 308, 393, 588, 756, 972, 1244
TscAI CASTG 3 cut(s) 330, 906, 1167
TseFI GTSAC 2 cut(s) 1068, 1147
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 1068, 1147
TspDTI ATGAA 3 cut(s) 391, 813, 1260
TspGWI ACGGA 1 cut(s) 1269
TspRI CASTG 3 cut(s) 330, 906, 1167
Van91I CCANNNNNTGG 2 cut(s) 338, 1057
VpaK11BI GGWCC 2 cut(s) 387, 1207
XapI RAATTY 1 cut(s) 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XhoI CTCGAG 2 cut(s) 193, 506
XmiI GTMKAC 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 544
XspI CTAG 4 cut(s) 245, 563, 923, 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.