MD09G1097600.v1.1

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005947
Physical Location & Seq
Forward (+)
7088497 .. 7089915
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1097600.v1.1.491

Sequence Viewer

Length: 1419 bp
ATGGCTTCCACACTTAAGGTCCTAGAGCAATGTCAGGTCTCTCCACCAAAAGATTCAGTTCCCACAACCTCTCTTCCCCTCACATTTTTGGACATACCTTGGCTTGTCTTGCTACAACCTGTCCAACGCCTCTACTTCTATGAATTCCCTCACCCATCTCACCATTTCACACAAACCCTCCTTCCATCAATCAAACACACTCTCTCCCTCACTCTCCAGAACTTCTTCCCGCTCTCTGGAAACCTCACTTGTCCCCCTCCTCCCTCCATGCCTTACATCCACTACGTCGCAAACATGTCTTCTGTCAAATTCACCATTACTGAGTCCGCTGCCAACTTCATCCATCTTATAGGCAACCACCCAAGACATGTCCAAGAGTTTGATCACCTTGTTCCCCATTTGCCGGATTTTAATAATTTGGACTCCAATTCAAGTGACACAACCACATTTCTACTTCCTATTCTTGCATTACAAGCAACAATTTTCCCAAACTCTGGCTTATGCATTTCCATAGCATATCGCCACGTTGTGGATGGACGAACTGCTAATCATTTCATGAAATGTTGGGCTTCTGTTTACAAGTCTGGAGGAGACTTATCTTGTATACATGAATCACCTCCTATCTATGACAGAGATTTGATCAAAACCCCAAAAGATTATGAGCCCATTTTATTCAATCAATACATCGGGTTGAGATCGACATGGAAAAATCTTGACATGGCCAAGTCTTCTTCCATTTTCATAGACAACTTTCGCACAACATTTGTACTAGGAAGAGAAAATATTCAAAGCCTGAAACAACTTATCATGACCCAATGCAAGAATTGTAATGAGCCAACCCCAAAGCACTTGTCGAAATTTGTGGTCACATGTGGTTTTGTGTGGGTATGCTTGATGAAGGCCCTACATGCTAGTGAAACTGGGGTGACTTCAATGAAGGATGATGACCTCTTACGGTTAGGGTTTGCAGCCGATATACGAAGCCTCTTTCAGAATCCAGTGCCCATCACCTATTTCGGAAACTGCTTGTCAGCATGTTATGTGGCAGATAAAAAGAGAGAGATCATGGCGGAGTGTGGGATTGCTAAGGCGGTGAAGGCCTTGGAGGCTGCAATTTTTGAGCTGAAAAATCGAGATCTTGAAGGGTTAAAGAGGATGGTTCCATTTCGAGGGGAGTTGGTTGTAGCAGAAAGTAGTGTAGCAATTGCTGGATCGCCAAAGTTGGGAGTTTATGGGACTGATTTTGGATGGGGAAGAGCAAAGAAAGTGGAGGTCGTTCACTTTGTTGATTCAACCGTTTTTTCTCTTGCTGAGAGTAGAGATGAGGAGGATGGCATTGAGGTTGGTTTAGCTCTACCGAGGACTACAATGGATGCATTTACTTCTTTGTTTGAACAAGGTCTGAAAAATTTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

473

Amino Acids

52.79

Weight (kDa)

6.6

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 16 - 465 1.9e-38 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 494, 529
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 604
AciI CCGC 4 cut(s) 230, 327, 1070, 1091
AclWI GGATC 1 cut(s) 1219
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 4 cut(s) 143, 308, 857, 1408
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 1 cut(s) 768
AfiI CCNNNNNNNGG 6 cut(s) 236, 403, 494, 529, 1169, 1223
AflII CTTAAG 1 cut(s) 14
AflIII ACRYGT 3 cut(s) 294, 367, 869
AgsI TTSAA 7 cut(s) 432, 676, 788, 933, 1142, 1293, 1394
AluBI AGCT 2 cut(s) 1123, 1352
AluI AGCT 2 cut(s) 1123, 1352
Alw26I GTCTC 2 cut(s) 43, 585
AlwI GGATC 1 cut(s) 1219
AoxI GGCC 3 cut(s) 720, 900, 1098
ApeKI GCWGC 3 cut(s) 329, 968, 1109
ApoI RAATTY 4 cut(s) 143, 308, 857, 1408
ArsI GACNNNNNNTTYG 2 cut(s) 836, 868
Asp700I GAANNNNTTC 3 cut(s) 224, 783, 1409
AspS9I GGNCC 2 cut(s) 19, 901
AsuHPI GGTGA 8 cut(s) 143, 152, 304, 377, 606, 937, 1000, 1105
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 1005
BalI TGGCCA 1 cut(s) 722
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 2 cut(s) 291, 720
BbvI GCAGC 3 cut(s) 316, 980, 1096
BccI CCATC 8 cut(s) 163, 193, 351, 527, 1013, 1150, 1242, 1325
BclI TGATCA 2 cut(s) 382, 639
BcoDI GTCTC 2 cut(s) 43, 585
BfaI CTAG 3 cut(s) 23, 770, 912
BfrI CTTAAG 1 cut(s) 14
BglI GCCNNNNNGGC 1 cut(s) 1106
BglII AGATCT 1 cut(s) 1135
BisI GCNGC 3 cut(s) 330, 969, 1110
BlsI GCNGC 3 cut(s) 331, 970, 1111
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 19, 901
BmiI GGNNCC 1 cut(s) 1161
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 1 cut(s) 1363
BmuI ACTGGG 1 cut(s) 930
BpiI GAAGAC 2 cut(s) 291, 720
BpmI CTGGAG 2 cut(s) 200, 606
Bpu10I CCTNAGC 1 cut(s) 1086
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 98, 1101, 1358
BsaXI ACNNNNNCTCC 4 cut(s) 162, 188, 192, 218
Bsc4I CCNNNNNNNGG 6 cut(s) 236, 403, 494, 529, 1169, 1223
Bse1I ACTGG 2 cut(s) 925, 998
Bse3DI GCAATG 1 cut(s) 35
BseDI CCNNGG 3 cut(s) 98, 1101, 1358
BseGI GGATG 8 cut(s) 276, 339, 538, 946, 1161, 1253, 1336, 1378
BseLI CCNNNNNNNGG 6 cut(s) 236, 403, 494, 529, 1169, 1223
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 312, 1302
BseNI ACTGG 2 cut(s) 925, 998
BseRI GAGGAG 3 cut(s) 249, 603, 1340
BseSI GKGCMC 1 cut(s) 1005
BseXI GCAGC 3 cut(s) 316, 980, 1096
BshFI GGCC 3 cut(s) 722, 902, 1100
BsiSI CCGG 1 cut(s) 404
BslFI GGGAC 2 cut(s) 237, 1249
BslI CCNNNNNNNGG 6 cut(s) 236, 403, 494, 529, 1169, 1223
BsmAI GTCTC 2 cut(s) 43, 585
BsmFI GGGAC 2 cut(s) 237, 1249
BsnI GGCC 3 cut(s) 722, 902, 1100
Bso31I GGTCTC 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 666, 1005
Bsp143I GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
BspACI CCGC 4 cut(s) 230, 327, 1070, 1091
BspANI GGCC 3 cut(s) 722, 902, 1100
BspCNI CTCAG 2 cut(s) 313, 1303
BspHI TCATGA 2 cut(s) 555, 807
BspLI GGNNCC 1 cut(s) 1161
BspPI GGATC 1 cut(s) 1219
BspQI GCTCTTC 1 cut(s) 1249
BspTI CTTAAG 1 cut(s) 14
BspTNI GGTCTC 1 cut(s) 43
BsrBI CCGCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 35
BsrI ACTGG 2 cut(s) 925, 998
BssECI CCNNGG 3 cut(s) 98, 1101, 1358
BssMI GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
BssNAI GTATAC 1 cut(s) 605
BssT1I CCWWGG 2 cut(s) 98, 1101
Bst1107I GTATAC 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 957, 1297
Bst6I CTCTTC 3 cut(s) 78, 769, 1249
BstAFI CTTAAG 1 cut(s) 14
BstDEI CTNAG 3 cut(s) 321, 1086, 1311
BstF5I GGATG 8 cut(s) 276, 339, 538, 946, 1161, 1253, 1336, 1378
BstKTI GATC 6 cut(s) 385, 642, 698, 1065, 1138, 1214
BstMAI GTCTC 2 cut(s) 43, 585
BstMBI GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
BstMWI GCNNNNNNNGC 5 cut(s) 109, 473, 908, 1097, 1106
BstNSI RCATGY 5 cut(s) 298, 371, 873, 911, 1038
BstSLI GKGCMC 1 cut(s) 1005
BstV1I GCAGC 3 cut(s) 316, 980, 1096
BstV2I GAAGAC 2 cut(s) 291, 720
BstX2I RGATCY 1 cut(s) 1135
BstYI RGATCY 1 cut(s) 1135
BstZ17I GTATAC 1 cut(s) 605
BsuRI GGCC 3 cut(s) 722, 902, 1100
BtsCI GGATG 8 cut(s) 276, 339, 538, 946, 1161, 1253, 1336, 1378
BtsIMutI CAGTG 1 cut(s) 1005
CciI TCATGA 2 cut(s) 555, 807
Cfr13I GGNCC 2 cut(s) 19, 901
Csp6I GTAC 1 cut(s) 767
CspCI CAANNNNNGTGG 2 cut(s) 1248, 1283
CviQI GTAC 1 cut(s) 767
DdeI CTNAG 3 cut(s) 321, 1086, 1311
DpnI GATC 6 cut(s) 384, 641, 697, 1064, 1137, 1213
DpnII GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 3 cut(s) 78, 769, 1249
EarI CTCTTC 3 cut(s) 78, 769, 1249
EciI GGCGGA 1 cut(s) 1085
Eco130I CCWWGG 2 cut(s) 98, 1101
Eco147I AGGCCT 1 cut(s) 1100
Eco24I GRGCYC 1 cut(s) 666
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 19
EcoO109I RGGNCCY 2 cut(s) 19, 901
EcoRI GAATTC 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 98, 1101
EcoT22I ATGCAT 2 cut(s) 506, 1378
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 2 cut(s) 98, 1101
FaqI GGGAC 2 cut(s) 237, 1249
FauI CCCGC 1 cut(s) 237
FbaI TGATCA 2 cut(s) 382, 639
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 3 cut(s) 330, 969, 1110
FokI GGATG 8 cut(s) 263, 326, 545, 953, 1168, 1260, 1343, 1385
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 3 cut(s) 330, 969, 1110
FspBI CTAG 3 cut(s) 23, 770, 912
GluI GCNGC 3 cut(s) 330, 969, 1110
GsuI CTGGAG 2 cut(s) 200, 606
HaeIII GGCC 3 cut(s) 722, 902, 1100
HapII CCGG 1 cut(s) 404
HinfI GANTC 6 cut(s) 53, 323, 422, 611, 994, 1289
HpaII CCGG 1 cut(s) 404
HphI GGTGA 8 cut(s) 143, 152, 304, 377, 606, 937, 1000, 1105
Hpy166II GTNNAC 3 cut(s) 577, 605, 1279
Hpy188I TCNGA 3 cut(s) 993, 1019, 1404
Hpy188III TCNNGA 8 cut(s) 217, 237, 556, 585, 713, 808, 1133, 1139
Hpy8I GTNNAC 3 cut(s) 577, 605, 1279
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 5 cut(s) 191, 892, 931, 1090, 1136
HpyCH4III ACNGT 2 cut(s) 957, 1297
HpyCH4IV ACGT 2 cut(s) 285, 525
HpyCH4V TGCA 6 cut(s) 467, 504, 819, 968, 1112, 1376
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 473, 908, 1097, 1106
HpyF3I CTNAG 3 cut(s) 321, 1086, 1311
HpySE526I ACGT 2 cut(s) 285, 525
Ksp22I TGATCA 2 cut(s) 382, 639
Kzo9I GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
LguI GCTCTTC 1 cut(s) 1249
Lsp1109I GCAGC 3 cut(s) 316, 980, 1096
LweI GCATC 1 cut(s) 1363
MaeI CTAG 3 cut(s) 23, 770, 912
MaeII ACGT 2 cut(s) 285, 525
MaeIII GTNAC 3 cut(s) 434, 865, 925
MalI GATC 6 cut(s) 384, 641, 697, 1064, 1137, 1213
MbiI CCGCTC 1 cut(s) 232
MboI GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
MboII GAAGA 7 cut(s) 65, 217, 291, 720, 723, 786, 1266
MfeI CAATTG 1 cut(s) 1203
MflI RGATCY 1 cut(s) 1135
MhlI GDGCHC 2 cut(s) 666, 1005
MlsI TGGCCA 1 cut(s) 722
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 2 cut(s) 332, 416
MmeI TCCRAC 1 cut(s) 148
Mox20I TGGCCA 1 cut(s) 722
Mph1103I ATGCAT 2 cut(s) 506, 1378
MroXI GAANNNNTTC 3 cut(s) 224, 783, 1409
MscI TGGCCA 1 cut(s) 722
MseI TTAA 3 cut(s) 15, 411, 1148
MslI CAYNNNNRTG 1 cut(s) 912
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 329
MspCI CTTAAG 1 cut(s) 14
MspI CCGG 1 cut(s) 404
MunI CAATTG 1 cut(s) 1203
MwoI GCNNNNNNNGC 5 cut(s) 109, 473, 908, 1097, 1106
NdeII GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
NlaIV GGNNCC 1 cut(s) 1161
NmuCI GTSAC 3 cut(s) 434, 865, 925
NsiI ATGCAT 2 cut(s) 506, 1378
NspI RCATGY 5 cut(s) 298, 371, 873, 911, 1038
PagI TCATGA 2 cut(s) 555, 807
PceI AGGCCT 1 cut(s) 1100
PciI ACATGT 3 cut(s) 294, 367, 869
PciSI GCTCTTC 1 cut(s) 1249
PdmI GAANNNNTTC 3 cut(s) 224, 783, 1409
PfeI GAWTC 4 cut(s) 53, 611, 994, 1289
PflMI CCANNNNNTGG 2 cut(s) 494, 529
PkrI GCNGC 3 cut(s) 331, 970, 1111
PleI GAGTC 2 cut(s) 331, 416
PpsI GAGTC 2 cut(s) 331, 416
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 3 cut(s) 294, 367, 869
Psp5II RGGWCCY 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 1161
PspPI GGNCC 2 cut(s) 19, 901
PspPPI RGGWCCY 1 cut(s) 19
PsuI RGATCY 1 cut(s) 1135
RsaI GTAC 1 cut(s) 768
RsaNI GTAC 1 cut(s) 767
RseI CAYNNNNRTG 1 cut(s) 912
SapI GCTCTTC 1 cut(s) 1249
SaqAI TTAA 3 cut(s) 15, 411, 1148
SatI GCNGC 3 cut(s) 330, 969, 1110
Sau3AI GATC 6 cut(s) 382, 639, 695, 1062, 1135, 1211
Sau96I GGNCC 2 cut(s) 19, 901
SchI GAGTC 2 cut(s) 332, 416
SduI GDGCHC 2 cut(s) 666, 1005
SfaNI GCATC 1 cut(s) 1363
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 1100
SsiI CCGC 4 cut(s) 230, 327, 1070, 1091
SspI AATATT 1 cut(s) 784
SspMI CTAG 3 cut(s) 23, 770, 912
StuI AGGCCT 1 cut(s) 1100
StyI CCWWGG 2 cut(s) 98, 1101
TaaI ACNGT 2 cut(s) 957, 1297
TaiI ACGT 2 cut(s) 288, 528
TaqI TCGA 4 cut(s) 698, 854, 1132, 1168
TatI WGTACW 1 cut(s) 766
TfiI GAWTC 4 cut(s) 53, 611, 994, 1289
Tru1I TTAA 3 cut(s) 15, 411, 1148
Tru9I TTAA 3 cut(s) 15, 411, 1148
TscAI CASTG 1 cut(s) 1005
TseFI GTSAC 3 cut(s) 434, 865, 925
TseI GCWGC 3 cut(s) 329, 968, 1109
Tsp45I GTSAC 3 cut(s) 434, 865, 925
TspDTI ATGAA 8 cut(s) 156, 328, 544, 572, 624, 730, 911, 950
TspRI CASTG 1 cut(s) 1005
Van91I CCANNNNNTGG 2 cut(s) 494, 529
Vha464I CTTAAG 1 cut(s) 14
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 4 cut(s) 143, 308, 857, 1408
XceI RCATGY 5 cut(s) 298, 371, 873, 911, 1038
XcmI CCANNNNNNNNNTGG 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 604
XmnI GAANNNNTTC 3 cut(s) 224, 783, 1409
XspI CTAG 3 cut(s) 23, 770, 912
Zsp2I ATGCAT 2 cut(s) 506, 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.