RLG00000030735

Anthocyanin 5-aromatic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005947
Physical Location & Seq
Reverse (-)
66651731 .. 66652435
705 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030735

Sequence Viewer

Length: 552 bp
ATGGGTAATACTAAAACTGCCAAGTTAGATCCAACGAGCATGGTTAGGGCAACTTTTTTGATGGACTTGACTCGCATGGAGAGGATCAAAAATTGGATTGTTAATCTATGCAACAAGGGAAATGAGCCACTACCGGTACATTTATCTGCTTATGTCCTAACATGGTTTGGCCGGGAAGCGGCGATAAGGAGTGAGTTGTTGGGGGACAGTGGAATTGTTGTTGCGGCAAGAGCAATTGGAAATACGGTTAAGAAGTTAGACAAGGCTATTTTTGGAGGGGCAGAGAAATGGATTTTGGATTGGAATATTATGTTTGAGTCGGAGCTCCATGTTATGGTATCAGGATCACCGAAAGTGGATCTTTATGAAACGGATTTTGGGTCGGGGAGACCGAAAAAGATAGAGGACGTTGGAGTTGATGGTACTAAAGCTATTGCACTGACTGAGAGTAGAGACGTATCAGGTGGCATTGAGGTTGGATTAGTGCTACCCAAGCCTCAAATGGATTATTTCTCAACTTTTTTCATTGAGGGACTGAATGCTACTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

184

Amino Acids

20.11

Weight (kDa)

5.75

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 71 - 172 6.1e-08 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 2 cut(s) 179, 224
AclWI GGATC 4 cut(s) 23, 92, 352, 366
AcoI YGGCCR 1 cut(s) 169
AfaI GTAC 2 cut(s) 138, 424
AfiI CCNNNNNNNGG 2 cut(s) 178, 334
AgeI ACCGGT 1 cut(s) 133
AjuI GAANNNNNNNTTGG 4 cut(s) 278, 310, 360, 392
AluBI AGCT 2 cut(s) 325, 431
AluI AGCT 2 cut(s) 325, 431
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 2 cut(s) 382, 447
AlwI GGATC 4 cut(s) 23, 92, 352, 366
AoxI GGCC 1 cut(s) 169
AsiGI ACCGGT 1 cut(s) 133
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 339
BanII GRGCYC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 327
BccI CCATC 2 cut(s) 55, 413
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 382, 447
BisI GCNGC 2 cut(s) 180, 225
BlsI GCNGC 2 cut(s) 181, 226
Bme1390I CCNGG 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 382
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 334
Bse118I RCCGGY 1 cut(s) 133
BseLI CCNNNNNNNGG 2 cut(s) 178, 334
BseMII CTCAG 1 cut(s) 435
BshFI GGCC 1 cut(s) 171
BshTI ACCGGT 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 2 cut(s) 134, 172
BslFI GGGAC 2 cut(s) 218, 546
BslI CCNNNNNNNGG 2 cut(s) 178, 334
BsmAI GTCTC 2 cut(s) 382, 447
BsmBI CGTCTC 1 cut(s) 447
BsmFI GGGAC 2 cut(s) 218, 546
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 1 cut(s) 171
Bso31I GGTCTC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 4 cut(s) 28, 84, 344, 358
BspACI CCGC 2 cut(s) 179, 224
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 1 cut(s) 436
BspPI GGATC 4 cut(s) 23, 92, 352, 366
BspTNI GGTCTC 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 133
BssAI RCCGGY 1 cut(s) 133
BssMI GATC 4 cut(s) 28, 84, 344, 358
Bst4CI ACNGT 2 cut(s) 209, 247
BstDEI CTNAG 1 cut(s) 444
BstKTI GATC 4 cut(s) 31, 87, 347, 361
BstMAI GTCTC 2 cut(s) 382, 447
BstMBI GATC 4 cut(s) 28, 84, 344, 358
BstMWI GCNNNNNNNGC 2 cut(s) 230, 493
BstSCI CCNGG 1 cut(s) 171
BstX2I RGATCY 2 cut(s) 28, 358
BstYI RGATCY 2 cut(s) 28, 358
BsuRI GGCC 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 214, 437
Cfr10I RCCGGY 1 cut(s) 133
Csp6I GTAC 2 cut(s) 137, 423
CspAI ACCGGT 1 cut(s) 133
CviAII CATG 5 cut(s) 40, 76, 162, 329, 549
CviJI RGCY 6 cut(s) 127, 171, 266, 325, 431, 496
CviKI_1 RGCY 6 cut(s) 127, 171, 266, 325, 431, 496
CviQI GTAC 2 cut(s) 137, 423
DdeI CTNAG 1 cut(s) 444
DpnI GATC 4 cut(s) 30, 86, 346, 360
DpnII GATC 4 cut(s) 28, 84, 344, 358
EaeI YGGCCR 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 5 cut(s) 43, 79, 165, 332, 552
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FaqI GGGAC 2 cut(s) 218, 546
FatI CATG 5 cut(s) 39, 75, 161, 328, 548
Fnu4HI GCNGC 2 cut(s) 180, 225
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 2 cut(s) 180, 225
GluI GCNGC 2 cut(s) 180, 225
HaeIII GGCC 1 cut(s) 171
HapII CCGG 2 cut(s) 134, 172
Hin1II CATG 5 cut(s) 43, 79, 165, 332, 552
HinfI GANTC 2 cut(s) 70, 317
HpaII CCGG 2 cut(s) 134, 172
HphI GGTGA 1 cut(s) 339
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 1 cut(s) 342
HpyCH4III ACNGT 2 cut(s) 209, 247
HpyCH4IV ACGT 2 cut(s) 408, 456
HpyCH4V TGCA 2 cut(s) 111, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 493
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 2 cut(s) 408, 456
Hsp92II CATG 5 cut(s) 43, 79, 165, 332, 552
Kzo9I GATC 4 cut(s) 28, 84, 344, 358
LmnI GCTCC 2 cut(s) 322, 330
LpnPI CCDG 4 cut(s) 147, 185, 327, 447
MaeII ACGT 2 cut(s) 408, 456
MalI GATC 4 cut(s) 30, 86, 346, 360
MboI GATC 4 cut(s) 28, 84, 344, 358
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 2 cut(s) 28, 358
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 3 cut(s) 91, 213, 234
MlyI GAGTC 2 cut(s) 64, 326
MmeI TCCRAC 4 cut(s) 56, 300, 391, 457
MnlI CCTC 6 cut(s) 75, 269, 397, 466, 507, 523
MseI TTAA 2 cut(s) 102, 249
MspI CCGG 2 cut(s) 134, 172
MspR9I CCNGG 1 cut(s) 173
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 544
MwoI GCNNNNNNNGC 2 cut(s) 230, 493
NciI CCSGG 1 cut(s) 173
NdeII GATC 4 cut(s) 28, 84, 344, 358
NlaIII CATG 5 cut(s) 43, 79, 165, 332, 552
PcsI WCGNNNNNNNCGW 1 cut(s) 389
PctI GAATGC 1 cut(s) 544
PflMI CCANNNNNTGG 1 cut(s) 334
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 2 cut(s) 181, 226
PleI GAGTC 2 cut(s) 64, 325
PpsI GAGTC 2 cut(s) 64, 325
Psp124BI GAGCTC 1 cut(s) 327
PsuI RGATCY 2 cut(s) 28, 358
RsaI GTAC 2 cut(s) 138, 424
RsaNI GTAC 2 cut(s) 137, 423
SacI GAGCTC 1 cut(s) 327
SaqAI TTAA 2 cut(s) 102, 249
SatI GCNGC 2 cut(s) 180, 225
Sau3AI GATC 4 cut(s) 28, 84, 344, 358
SchI GAGTC 2 cut(s) 64, 326
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 1 cut(s) 327
SetI ASST 6 cut(s) 327, 411, 433, 459, 466, 477
Sse9I AATT 3 cut(s) 91, 213, 234
SsiI CCGC 2 cut(s) 179, 224
SspI AATATT 1 cut(s) 307
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 209, 247
TaiI ACGT 2 cut(s) 411, 459
TaqII GACCGA 1 cut(s) 406
TasI AATT 3 cut(s) 91, 213, 234
TauI GCSGC 2 cut(s) 182, 227
Tru1I TTAA 2 cut(s) 102, 249
Tru9I TTAA 2 cut(s) 102, 249
TscAI CASTG 2 cut(s) 214, 444
TspDTI ATGAA 2 cut(s) 381, 514
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 2 cut(s) 214, 444
Van91I CCANNNNNTGG 1 cut(s) 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.