Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0002765

Physical Location & Seq

Genomic Coordinates Reverse (-)

3753933 .. 3757418

Gene Structure 3486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1043700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1200 bp

ATGGAGATTGGTGATACAAGAGAGAGTATTGTAATTGTGGGTGGTGGGATTTGCGGCCTTGCCACTGCTCTTGCTCTTCACAGGAAAGGCATTAGAAGCGTGGTCCTGGAAAGATCAAACAGATTGCCAGCAACCGGGGTAGCTATTATCGTGCATCTAAACGGCTGGCGTGCACTTGATCAGCTTGGTGTTGCCTCACTCCTTAGACAAACTTCTTTTCCTATACTCTCCGGACAACTTATTTCACTCGGTAGTGGTGAAATTGAAAATATGGATGTAGGGAAAGAAGAACTGAGAGTTTTGAAGAGGACTGATCTCGTTAGTGTTTTGGCTGATAACTTGCCTCCAAACACTGTACGTTTCGGGTGCGAAGTGCATTCTGTTAAGTTGAATCCCGGAACTTCTTCTCCAGTTCTTCAACTTCAAGATGGAACAATTTTGAACCCCAAGGTTGTGATTGGATGCGACGGAGTGAACTCAATCGTATCAAATGTGATGGGGATGAAGGCTTCTAATATTTTCAGAATATGTGTCATAAGAGGCTTCACAAGATATCCAGATGGTCATGAATTCGGTAGTGAGTTCACTTTAACGAGAAAAGATGATACTCAGGTGGGACGACTCCCCATGACCGAAAGCCTGGTGTATTGGTTCATGACTCGAAAACATTCTTCTCAAGATTCGGCAGCTTCTAAGGACCAGAAACTAATTCGGGAGTTGGGAGTGAAATCGGTGGAGGGCTTCCCTACAAGCATCATAGAGATGTTTAAGAATTGCGAGCTAGACTCTATACATCTCACAGAGTACGTGAGATACCATGCACCATGGGACATACTACGAATACCCTCTCGCGCGGGGACTGTGTCACTGGCGGGAGATGCCATGCATACCATGGGACCTTTCCTTGCACAAGGTGGCTCAGCCGCACTAGAAGATGCAGTTGTGCTTGCTCGGTGCCTAGCTCAGAAAACTCGCGTTGATCTAAGAGGAAGAGGAACTAAACTGATGGTAGAGGAAGCGTTGGATCAGTATGTGAAAGAGCGAAAGACTCGAGTTCTGCGATTGTCTTTGCAGACATACCTGATTGGGAAAATGTTTCATACTTCATCACAGTTTGTTAAGCTCATATGCATTGTCCTTCTGGCAGTCCTGTTTAGCGAGTCACACGGCCATACCCGCTACGACTGCGGCAGTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_3

Protein Analysis

400

Amino Acids

43.82

Weight (kDa)

8.86

Isoelectric Point (pI)

34.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_3	PF01494	10 - 352	2.6e-28	FAD binding domain
DAO	PF01266	10 - 43	3e-06	FAD dependent oxidoreductase
NAD_binding_8	PF13450	12 - 42	1e-05	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15760 AT4G15760
fragaria_vesca	FvH4_7g03910
malus_domestica	MD07G1043700.v1.1 MD10G1041900.v1.1 MD10G1042000.v1.1
prunus_persica	Prupe.2G043900_v2.0.a1 Prupe.8G046000_v2.0.a1 Prupe.8G046000_v2.0.a1 Prupe.8G046000_v2.0.a1 Prupe.8G046000_v2.0.a1 Prupe.8G046000_v2.0.a1
pyrus_communis	pycom10g02900 pycom10g02910
rosa_chinensis	RchiOBHm_Chr1g0327481 RchiOBHm_Chr1g0327491 RchiOBHm_Chr2g0087851
rosa_laevigata	RLG00000015907 RLG00000029990
rosa_multiflora	Rmu_sc0003600.1_g000007 Rmu_sc0008385.1_g000017 Rmu_ssc0000042.1_g000053
rosa_roxburghii	Rroxscaffold_2G00151770 Rroxscaffold_2G00153170 Rroxscaffold_4G00322630
rosa_rugosa	Rorug01G0067300 Rorug01G0479000
rosa_samantha	Rh1AG084300 Rh1BG067000 Rh1CG082000 Rh1CG082100 Rh1DG088600 Rh1DG088700 Rh2AG033600 Rh2BG032800 Rh2CG033500 Rh2DG033200
rosa_wichuraiana	Rw1G006630 Rw2G002620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	954
AccII	CGCG	3 cut(s)	852, 854, 975
AccIII	TCCGGA	1 cut(s)	230
AciI	CCGC	6 cut(s)	54, 854, 872, 924, 1177, 1188
AclWI	GGATC	1 cut(s)	1032
AcoI	YGGCCR	1 cut(s)	1168
AcsI	RAATTY	1 cut(s)	569
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	2 cut(s)	357, 806
AfiI	CCNNNNNNNGG	1 cut(s)	134
AgsI	TTSAA	6 cut(s)	266, 304, 391, 419, 425, 442
AjnI	CCWGG	2 cut(s)	105, 639
AloI	GAACNNNNNNTCC	2 cut(s)	391, 423
AluBI	AGCT	6 cut(s)	143, 184, 689, 781, 962, 1123
AluI	AGCT	6 cut(s)	143, 184, 689, 781, 962, 1123
Alw21I	GWGCWC	1 cut(s)	175
Alw44I	GTGCAC	1 cut(s)	171
AlwI	GGATC	1 cut(s)	1032
Ama87I	CYCGRG	1 cut(s)	1050
Aor13HI	TCCGGA	1 cut(s)	230
AoxI	GGCC	2 cut(s)	55, 1168
ApaLI	GTGCAC	1 cut(s)	171
ApeKI	GCWGC	1 cut(s)	686
ApoI	RAATTY	1 cut(s)	569
Asp700I	GAANNNNTTC	2 cut(s)	403, 667
AspLEI	GCGC	1 cut(s)	854
AspS9I	GGNCC	3 cut(s)	103, 697, 896
AsuC2I	CCSGG	2 cut(s)	136, 396
AsuHPI	GGTGA	2 cut(s)	23, 269
AvaI	CYCGRG	1 cut(s)	1050
AvaII	GGWCC	3 cut(s)	103, 697, 896
BaeGI	GKGCMC	1 cut(s)	175
BaeI	ACNNNNGTAYC	2 cut(s)	597, 630
BanI	GGYRCC	1 cut(s)	954
Bbv12I	GWGCWC	1 cut(s)	175
BbvI	GCAGC	1 cut(s)	698
BccI	CCATC	4 cut(s)	422, 490, 554, 1000
BceAI	ACGGC	2 cut(s)	178, 1183
BciT130I	CCWGG	2 cut(s)	107, 641
BclI	TGATCA	1 cut(s)	178
BcnI	CCSGG	2 cut(s)	136, 396
BfaI	CTAG	3 cut(s)	782, 929, 959
BisI	GCNGC	4 cut(s)	55, 687, 924, 1189
BlpI	GCTNAGC	1 cut(s)	919
BlsI	GCNGC	4 cut(s)	56, 688, 925, 1190
Bme1390I	CCNGG	4 cut(s)	107, 136, 396, 641
Bme18I	GGWCC	3 cut(s)	103, 697, 896
BmeT110I	CYCGRG	1 cut(s)	1050
BmgT120I	GGNCC	3 cut(s)	103, 697, 896
BmiI	GGNNCC	2 cut(s)	897, 956
BmrFI	CCNGG	4 cut(s)	107, 136, 396, 641
BmsI	GCATC	5 cut(s)	163, 452, 762, 868, 925
BplI	GAGNNNNNCTC	2 cut(s)	770, 802
BpmI	CTGGAG	1 cut(s)	393
Bpu1102I	GCTNAGC	1 cut(s)	919
BpuEI	CTTGAG	1 cut(s)	660
BpuMI	CCSGG	2 cut(s)	136, 396
BsaAI	YACGTR	1 cut(s)	808
BsaJI	CCNNGG	4 cut(s)	135, 447, 824, 891
BsaWI	WCCGGW	1 cut(s)	230
BsaXI	ACNNNNNCTCC	2 cut(s)	391, 421
Bsc4I	CCNNNNNNNGG	1 cut(s)	134
Bse1I	ACTGG	2 cut(s)	410, 873
BseAI	TCCGGA	1 cut(s)	230
BseBI	CCWGG	2 cut(s)	107, 641
BseDI	CCNNGG	4 cut(s)	135, 447, 824, 891
BseGI	GGATG	3 cut(s)	280, 467, 507
BseLI	CCNNNNNNNGG	1 cut(s)	134
BseMII	CTCAG	4 cut(s)	284, 623, 933, 977
BseNI	ACTGG	2 cut(s)	410, 873
BseSI	GKGCMC	1 cut(s)	175
BseXI	GCAGC	1 cut(s)	698
Bsh1236I	CGCG	3 cut(s)	852, 854, 975
BshFI	GGCC	2 cut(s)	57, 1170
BshNI	GGYRCC	1 cut(s)	954
BsiHKAI	GWGCWC	1 cut(s)	175
BsiHKCI	CYCGRG	1 cut(s)	1050
BsiSI	CCGG	3 cut(s)	135, 231, 396
BslFI	GGGAC	4 cut(s)	630, 842, 871, 909
BslI	CCNNNNNNNGG	1 cut(s)	134
BsmFI	GGGAC	4 cut(s)	630, 842, 871, 909
BsmI	GAATGC	1 cut(s)	376
BsnI	GGCC	2 cut(s)	57, 1170
BsoBI	CYCGRG	1 cut(s)	1050
Bsp1286I	GDGCHC	1 cut(s)	175
Bsp13I	TCCGGA	1 cut(s)	230
Bsp143I	GATC	5 cut(s)	113, 178, 313, 979, 1024
Bsp1720I	GCTNAGC	1 cut(s)	919
Bsp19I	CCATGG	2 cut(s)	824, 891
BspACI	CCGC	6 cut(s)	54, 854, 872, 924, 1177, 1188
BspANI	GGCC	2 cut(s)	57, 1170
BspCNI	CTCAG	4 cut(s)	285, 622, 932, 976
BspEI	TCCGGA	1 cut(s)	230
BspFNI	CGCG	3 cut(s)	852, 854, 975
BspHI	TCATGA	2 cut(s)	565, 654
BspLI	GGNNCC	2 cut(s)	897, 956
BspPI	GGATC	1 cut(s)	1032
BspQI	GCTCTTC	1 cut(s)	81
BspT107I	GGYRCC	1 cut(s)	954
BsrI	ACTGG	2 cut(s)	410, 873
BssECI	CCNNGG	4 cut(s)	135, 447, 824, 891
BssMI	GATC	5 cut(s)	113, 178, 313, 979, 1024
BssT1I	CCWWGG	3 cut(s)	447, 824, 891
Bst2UI	CCWGG	2 cut(s)	107, 641
Bst4CI	ACNGT	3 cut(s)	355, 862, 1113
Bst6I	CTCTTC	3 cut(s)	81, 299, 985
BstBAI	YACGTR	1 cut(s)	808
BstC8I	GCNNGC	5 cut(s)	129, 167, 171, 779, 948
BstDEI	CTNAG	7 cut(s)	203, 293, 609, 693, 919, 963, 983
BstDSI	CCRYGG	2 cut(s)	824, 891
BstF5I	GGATG	3 cut(s)	280, 467, 507
BstFNI	CGCG	3 cut(s)	852, 854, 975
BstHHI	GCGC	1 cut(s)	854
BstKTI	GATC	5 cut(s)	116, 181, 316, 982, 1027
BstMBI	GATC	5 cut(s)	113, 178, 313, 979, 1024
BstMWI	GCNNNNNNNGC	4 cut(s)	96, 878, 1176, 1185
BstNI	CCWGG	2 cut(s)	107, 641
BstSCI	CCNGG	4 cut(s)	105, 134, 394, 639
BstSLI	GKGCMC	1 cut(s)	175
BstUI	CGCG	3 cut(s)	852, 854, 975
BstV1I	GCAGC	1 cut(s)	698
BsuRI	GGCC	2 cut(s)	57, 1170
BtgI	CCRYGG	2 cut(s)	824, 891
BtsCI	GGATG	3 cut(s)	280, 467, 507
BtsI	GCAGTG	1 cut(s)	63
BtsIMutI	CAGTG	3 cut(s)	63, 351, 866
Cac8I	GCNNGC	5 cut(s)	129, 167, 171, 779, 948
CciI	TCATGA	2 cut(s)	565, 654
CfoI	GCGC	1 cut(s)	854
Cfr13I	GGNCC	3 cut(s)	103, 697, 896
Csp6I	GTAC	2 cut(s)	356, 805
CspCI	CAANNNNNGTGG	2 cut(s)	52, 87
CviAII	CATG	7 cut(s)	566, 628, 655, 818, 825, 883, 892
CviQI	GTAC	2 cut(s)	356, 805
DdeI	CTNAG	7 cut(s)	203, 293, 609, 693, 919, 963, 983
DpnI	GATC	5 cut(s)	115, 180, 315, 981, 1026
DpnII	GATC	5 cut(s)	113, 178, 313, 979, 1024
DraIII	CACNNNGTG	1 cut(s)	914
EaeI	YGGCCR	1 cut(s)	1168
Eam1104I	CTCTTC	3 cut(s)	81, 299, 985
EarI	CTCTTC	3 cut(s)	81, 299, 985
Eco130I	CCWWGG	3 cut(s)	447, 824, 891
Eco32I	GATATC	1 cut(s)	554
Eco47I	GGWCC	3 cut(s)	103, 697, 896
Eco88I	CYCGRG	1 cut(s)	1050
EcoO109I	RGGNCCY	1 cut(s)	896
EcoRI	GAATTC	1 cut(s)	569
EcoRII	CCWGG	2 cut(s)	105, 639
EcoRV	GATATC	1 cut(s)	554
EcoT14I	CCWWGG	3 cut(s)	447, 824, 891
EcoT22I	ATGCAT	2 cut(s)	888, 1133
ErhI	CCWWGG	3 cut(s)	447, 824, 891
FaeI	CATG	7 cut(s)	569, 631, 658, 821, 828, 886, 895
FaqI	GGGAC	4 cut(s)	630, 842, 871, 909
FatI	CATG	7 cut(s)	565, 627, 654, 817, 824, 882, 891
FauI	CCCGC	3 cut(s)	847, 865, 1184
FauNDI	CATATG	1 cut(s)	1127
FbaI	TGATCA	1 cut(s)	178
Fnu4HI	GCNGC	4 cut(s)	55, 687, 924, 1189
FokI	GGATG	3 cut(s)	287, 474, 514
Fsp4HI	GCNGC	4 cut(s)	55, 687, 924, 1189
FspBI	CTAG	3 cut(s)	782, 929, 959
GlaI	GCGC	1 cut(s)	853
GluI	GCNGC	4 cut(s)	55, 687, 924, 1189
GsuI	CTGGAG	1 cut(s)	393
HaeIII	GGCC	2 cut(s)	57, 1170
HapII	CCGG	3 cut(s)	135, 231, 396
HhaI	GCGC	1 cut(s)	854
Hin1II	CATG	7 cut(s)	569, 631, 658, 821, 828, 886, 895
Hin6I	GCGC	1 cut(s)	852
HinP1I	GCGC	1 cut(s)	852
HinfI	GANTC	7 cut(s)	391, 621, 658, 680, 785, 1048, 1160
HpaII	CCGG	3 cut(s)	135, 231, 396
HphI	GGTGA	2 cut(s)	23, 269
Hpy166II	GTNNAC	3 cut(s)	173, 475, 585
Hpy188I	TCNGA	2 cut(s)	524, 966
Hpy188III	TCNNGA	7 cut(s)	231, 425, 557, 566, 655, 677, 713
Hpy8I	GTNNAC	3 cut(s)	173, 475, 585
Hpy99I	CGWCG	1 cut(s)	470
HpyAV	CCTTC	2 cut(s)	499, 1148
HpyCH4III	ACNGT	3 cut(s)	355, 862, 1113
HpyCH4IV	ACGT	2 cut(s)	358, 807
HpyCH4V	TGCA	9 cut(s)	154, 173, 376, 821, 886, 908, 938, 1072, 1131
HpyF10VI	GCNNNNNNNGC	4 cut(s)	96, 878, 1176, 1185
HpyF3I	CTNAG	7 cut(s)	203, 293, 609, 693, 919, 963, 983
HpySE526I	ACGT	2 cut(s)	358, 807
Hsp92II	CATG	7 cut(s)	569, 631, 658, 821, 828, 886, 895
HspAI	GCGC	1 cut(s)	852
Kpn2I	TCCGGA	1 cut(s)	230
Ksp22I	TGATCA	1 cut(s)	178
Kzo9I	GATC	5 cut(s)	113, 178, 313, 979, 1024
LguI	GCTCTTC	1 cut(s)	81
Lsp1109I	GCAGC	1 cut(s)	698
LweI	GCATC	5 cut(s)	163, 452, 762, 868, 925
MaeI	CTAG	3 cut(s)	782, 929, 959
MaeII	ACGT	2 cut(s)	358, 807
MaeIII	GTNAC	2 cut(s)	864, 1161
MalI	GATC	5 cut(s)	115, 180, 315, 981, 1026
MboI	GATC	5 cut(s)	113, 178, 313, 979, 1024
MboII	GAAGA	8 cut(s)	68, 299, 316, 396, 407, 663, 944, 1002
MhlI	GDGCHC	1 cut(s)	175
MluCI	AATT	6 cut(s)	33, 261, 435, 569, 708, 772
MlyI	GAGTC	5 cut(s)	615, 652, 779, 1042, 1169
MmeI	TCCRAC	1 cut(s)	1002
MnlI	CCTC	9 cut(s)	205, 300, 354, 533, 730, 856, 980, 986, 1006
Mph1103I	ATGCAT	2 cut(s)	888, 1133
MroI	TCCGGA	1 cut(s)	230
MroXI	GAANNNNTTC	2 cut(s)	403, 667
MseI	TTAA	4 cut(s)	384, 590, 768, 1119
MslI	CAYNNNNRTG	1 cut(s)	761
MspI	CCGG	3 cut(s)	135, 231, 396
MspR9I	CCNGG	4 cut(s)	107, 136, 396, 641
Mva1269I	GAATGC	1 cut(s)	376
MvaI	CCWGG	2 cut(s)	107, 641
MvnI	CGCG	3 cut(s)	852, 854, 975
MwoI	GCNNNNNNNGC	4 cut(s)	96, 878, 1176, 1185
NciI	CCSGG	2 cut(s)	136, 396
NcoI	CCATGG	2 cut(s)	824, 891
NdeI	CATATG	1 cut(s)	1127
NdeII	GATC	5 cut(s)	113, 178, 313, 979, 1024
NlaIII	CATG	7 cut(s)	569, 631, 658, 821, 828, 886, 895
NlaIV	GGNNCC	2 cut(s)	897, 956
NmuCI	GTSAC	2 cut(s)	864, 1161
NsiI	ATGCAT	2 cut(s)	888, 1133
PaeR7I	CTCGAG	1 cut(s)	1050
PagI	TCATGA	2 cut(s)	565, 654
PciSI	GCTCTTC	1 cut(s)	81
PcsI	WCGNNNNNNNCGW	1 cut(s)	1057
PctI	GAATGC	1 cut(s)	376
PdmI	GAANNNNTTC	2 cut(s)	403, 667
PfeI	GAWTC	2 cut(s)	391, 680
PflFI	GACNNNGTC	1 cut(s)	862
PfoI	TCCNGGA	2 cut(s)	105, 394
PkrI	GCNGC	4 cut(s)	56, 688, 925, 1190
PleI	GAGTC	5 cut(s)	615, 652, 779, 1042, 1168
PpsI	GAGTC	5 cut(s)	615, 652, 779, 1042, 1168
Ppu21I	YACGTR	1 cut(s)	808
PpuMI	RGGWCCY	1 cut(s)	896
Psp5II	RGGWCCY	1 cut(s)	896
Psp6I	CCWGG	2 cut(s)	105, 639
PspGI	CCWGG	2 cut(s)	105, 639
PspN4I	GGNNCC	2 cut(s)	897, 956
PspPI	GGNCC	3 cut(s)	103, 697, 896
PspPPI	RGGWCCY	1 cut(s)	896
PspXI	VCTCGAGB	1 cut(s)	1050
PsyI	GACNNNGTC	1 cut(s)	862
RsaI	GTAC	2 cut(s)	357, 806
RsaNI	GTAC	2 cut(s)	356, 805
RseI	CAYNNNNRTG	1 cut(s)	761
SapI	GCTCTTC	1 cut(s)	81
SaqAI	TTAA	4 cut(s)	384, 590, 768, 1119
SatI	GCNGC	4 cut(s)	55, 687, 924, 1189
Sau3AI	GATC	5 cut(s)	113, 178, 313, 979, 1024
Sau96I	GGNCC	3 cut(s)	103, 697, 896
SchI	GAGTC	5 cut(s)	615, 652, 779, 1042, 1169
ScrFI	CCNGG	4 cut(s)	107, 136, 396, 641
SduI	GDGCHC	1 cut(s)	175
SfaNI	GCATC	5 cut(s)	163, 452, 762, 868, 925
Sfr274I	CTCGAG	1 cut(s)	1050
SinI	GGWCC	3 cut(s)	103, 697, 896
SlaI	CTCGAG	1 cut(s)	1050
SmiMI	CAYNNNNRTG	1 cut(s)	761
SmlI	CTYRAG	2 cut(s)	675, 1050
SmoI	CTYRAG	2 cut(s)	675, 1050
Sse9I	AATT	6 cut(s)	33, 261, 435, 569, 708, 772
SsiI	CCGC	6 cut(s)	54, 854, 872, 924, 1177, 1188
SspI	AATATT	1 cut(s)	517
SspMI	CTAG	3 cut(s)	782, 929, 959
StyD4I	CCNGG	4 cut(s)	105, 134, 394, 639
StyI	CCWWGG	3 cut(s)	447, 824, 891
TaaI	ACNGT	3 cut(s)	355, 862, 1113
TaiI	ACGT	2 cut(s)	361, 810
TaqI	TCGA	2 cut(s)	661, 1051
TaqII	GACCGA	1 cut(s)	647
TasI	AATT	6 cut(s)	33, 261, 435, 569, 708, 772
TauI	GCSGC	3 cut(s)	57, 926, 1191
TfiI	GAWTC	2 cut(s)	391, 680
Tru1I	TTAA	4 cut(s)	384, 590, 768, 1119
Tru9I	TTAA	4 cut(s)	384, 590, 768, 1119
TscAI	CASTG	3 cut(s)	70, 358, 873
TseFI	GTSAC	2 cut(s)	864, 1161
TseI	GCWGC	1 cut(s)	686
Tsp45I	GTSAC	2 cut(s)	864, 1161
TspDTI	ATGAA	5 cut(s)	518, 582, 643, 1088, 1095
TspGWI	ACGGA	1 cut(s)	483
TspRI	CASTG	3 cut(s)	70, 358, 873
Tth111I	GACNNNGTC	1 cut(s)	862
VneI	GTGCAC	1 cut(s)	171
VpaK11BI	GGWCC	3 cut(s)	103, 697, 896
XapI	RAATTY	1 cut(s)	569
XcmI	CCANNNNNNNNNTGG	1 cut(s)	889
XhoI	CTCGAG	1 cut(s)	1050
XmnI	GAANNNNTTC	2 cut(s)	403, 667
XspI	CTAG	3 cut(s)	782, 929, 959
Zsp2I	ATGCAT	2 cut(s)	888, 1133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.