MD10G1042000.v1.1

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002765
Physical Location & Seq
Reverse (-)
5423294 .. 5425656
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1042000.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGAAGTAGGCGATGAAGAGATAGGGATCGCCATTGTTGGTGGTGGAATCTGCGGCCTCGCAACTGCCCTTGCTCTTCATAGAAAGGGGTTTACAAGTGTAGTGTTGGAAAGATCAGAATCCCTACGTGCTACCGGAGGAGGTATCACTATTCGGGCAAATGGTTGGCGAGCCTTGGATGAGCTTGGTGTGGCCTCAAAGCTCAGACAAACTGCCCTACCACTTCAAGGGGCCCGCGACATATACCCTCATAATGGCAAGCAACAAAAAATATCATATGGAGGTGCGGAAGCACGATGCTTGAAACGGAGTGATCTGATCACAACACTAGCAGAGAGCTTGCCAGTTGGTACCATACGCCTTGGATGCCAAGCAATCTCTGTTAAGTTGGACTCGTTAACTTCCTATCCAACTCTTCAACTCCACAACGGAAGCACCATCAAAGCCACGGTTGTGATCGGATGTGATGGAACAAAATCAGTTGTTGCAGATTTCATCGGGGTAAAACCATCAAAGCCGTTCATGTTATCGGAGGTTAGAGGTTTTACAGTTTACGCAGGTGGTCATAATTTTGGAAACGAGTTTGTACAAGTTAAGGGTGACAAGAACACAATTGGAAGACTTCCAGTTCATGATAACTTGGTCTACTGGTTCGTCTCTCACCAAGTGCATGGCGGACAAGATCCGTCGAAGGTTTCAAAGGATCCAGAGCTCATTCGACAATTTACATTACAATCAATACTCGAGGAATTTCCTTCAGAAATGGTAGACATGATAAGAAAGAGTGAACTAGAATCAGTATCTCACGTGCGCTTGAGATATCGTCCGCCATGGGAGATAGTAGTACAAAATTTTCGAAAAGGAAGCGTGACGGTGGCTGGAGATGCCATGCATGTGATGGGGCCTTTCATCGGTCAGGGAGGCTCCGCAGGCATCGAAGACGCCATTGTTATTGCTAGAAGCTTGGCTCCCGCATTGGCCAAAAATTACTACAAAAAATGGAGTGGAAGGAATGGAATGATGGTTGAAGTGGGAGAGGCTTTCGACAAGTATGTGAAAGAGAGAAGGATGAGATTGGTGATGCTCTCTACACAAACTTATCTACTTGGTTTGCTGCAACAAGATTCAGGGTCGATGCTTAAATTTGTGTGTTTAATCTTGATGGCTACTCTATTTAGTGATGTAATTCGTCACACTCGATATGATTGTGGACGTCTTTAA

Protein Analysis

407

Amino Acids

44.43

Weight (kDa)

9.18

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 10 - 326 5.6e-27 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
AatII GACGTC 1 cut(s) 1216
Acc36I ACCTGC 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 350
AccB1I GGYRCC 1 cut(s) 350
AccI GTMKAC 2 cut(s) 645, 768
AccII CGCG 1 cut(s) 237
AciI CCGC 7 cut(s) 54, 235, 287, 675, 827, 927, 972
AclWI GGATC 4 cut(s) 35, 677, 698, 711
AcoI YGGCCR 1 cut(s) 978
AcsI RAATTY 3 cut(s) 749, 850, 1142
AcuI CTGAAG 1 cut(s) 741
AcvI CACGTG 1 cut(s) 808
AcyI GRCGYC 2 cut(s) 942, 1213
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 3 cut(s) 352, 588, 846
AfiI CCNNNNNNNGG 3 cut(s) 227, 254, 911
AgsI TTSAA 5 cut(s) 227, 304, 419, 699, 1028
AleI CACNNNNGTG 1 cut(s) 452
AluBI AGCT 5 cut(s) 184, 202, 339, 712, 963
AluI AGCT 5 cut(s) 184, 202, 339, 712, 963
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 4 cut(s) 35, 677, 698, 711
Ama87I CYCGRG 1 cut(s) 743
AoxI GGCC 5 cut(s) 55, 192, 231, 902, 978
ApaI GGGCCC 1 cut(s) 235
ApeKI GCWGC 1 cut(s) 1114
ApoI RAATTY 3 cut(s) 749, 850, 1142
Asp718I GGTACC 1 cut(s) 350
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 3 cut(s) 231, 232, 902
AsuHPI GGTGA 3 cut(s) 611, 653, 1090
AsuII TTCGAA 1 cut(s) 856
AvaI CYCGRG 1 cut(s) 743
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 980
BamHI GGATCC 1 cut(s) 703
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 2 cut(s) 235, 714
BbrPI CACGTG 1 cut(s) 808
BbsI GAAGAC 2 cut(s) 625, 945
Bbv12I GWGCWC 1 cut(s) 714
BbvI GCAGC 1 cut(s) 1101
BccI CCATC 6 cut(s) 446, 461, 517, 892, 1015, 1156
BceAI ACGGC 1 cut(s) 502
BclI TGATCA 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 3 cut(s) 329, 791, 957
BfuAI ACCTGC 1 cut(s) 548
BisI GCNGC 2 cut(s) 55, 1115
BlsI GCNGC 2 cut(s) 56, 1116
BmeT110I CYCGRG 1 cut(s) 743
BmgT120I GGNCC 3 cut(s) 231, 232, 902
BmiI GGNNCC 7 cut(s) 232, 233, 352, 705, 903, 925, 969
BmsI GCATC 6 cut(s) 287, 356, 874, 942, 1071, 1125
BpiI GAAGAC 2 cut(s) 625, 945
BpmI CTGGAG 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 856
BpuEI CTTGAG 1 cut(s) 835
BsaAI YACGTR 2 cut(s) 128, 808
BsaBI GATNNNNATC 2 cut(s) 26, 118
BsaHI GRCGYC 2 cut(s) 942, 1213
BsaJI CCNNGG 4 cut(s) 174, 361, 447, 830
BsaWI WCCGGW 1 cut(s) 134
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 254, 911
Bse1I ACTGG 3 cut(s) 344, 626, 653
Bse8I GATNNNNATC 2 cut(s) 26, 118
BseDI CCNNGG 4 cut(s) 174, 361, 447, 830
BseGI GGATG 4 cut(s) 184, 371, 467, 1074
BseJI GATNNNNATC 2 cut(s) 26, 118
BseLI CCNNNNNNNGG 3 cut(s) 227, 254, 911
BseMII CTCAG 1 cut(s) 217
BseNI ACTGG 3 cut(s) 344, 626, 653
BseRI GAGGAG 1 cut(s) 153
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 1 cut(s) 1101
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 5 cut(s) 57, 194, 233, 904, 980
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 743
BsiSI CCGG 1 cut(s) 135
BslI CCNNNNNNNGG 3 cut(s) 227, 254, 911
BsmAI GTCTC 1 cut(s) 661
BsmBI CGTCTC 1 cut(s) 661
BsnI GGCC 5 cut(s) 57, 194, 233, 904, 980
BsoBI CYCGRG 1 cut(s) 743
Bsp119I TTCGAA 1 cut(s) 856
Bsp120I GGGCCC 1 cut(s) 231
Bsp1286I GDGCHC 2 cut(s) 235, 714
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 7 cut(s) 54, 235, 287, 675, 827, 927, 972
BspANI GGCC 5 cut(s) 57, 194, 233, 904, 980
BspCNI CTCAG 1 cut(s) 216
BspFNI CGCG 1 cut(s) 237
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 7 cut(s) 232, 233, 352, 705, 903, 925, 969
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 4 cut(s) 35, 677, 698, 711
BspQI GCTCTTC 1 cut(s) 81
BspT104I TTCGAA 1 cut(s) 856
BspT107I GGYRCC 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 3 cut(s) 344, 626, 653
BssECI CCNNGG 4 cut(s) 174, 361, 447, 830
BssMI GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
BssNI GRCGYC 2 cut(s) 942, 1213
BssT1I CCWWGG 3 cut(s) 174, 361, 830
Bst4CI ACNGT 3 cut(s) 451, 550, 874
Bst6I CTCTTC 3 cut(s) 12, 81, 420
BstACI GRCGYC 2 cut(s) 942, 1213
BstAUI TGTACA 1 cut(s) 586
BstBAI YACGTR 2 cut(s) 128, 808
BstBI TTCGAA 1 cut(s) 856
BstC8I GCNNGC 5 cut(s) 171, 235, 260, 341, 931
BstDEI CTNAG 1 cut(s) 203
BstDSI CCRYGG 2 cut(s) 447, 830
BstF5I GGATG 4 cut(s) 184, 371, 467, 1074
BstFNI CGCG 1 cut(s) 237
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 7 cut(s) 30, 116, 316, 321, 459, 685, 706
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
BstMWI GCNNNNNNNGC 3 cut(s) 366, 884, 930
BstNSI RCATGY 1 cut(s) 896
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 1101
BstV2I GAAGAC 2 cut(s) 625, 945
BstX2I RGATCY 2 cut(s) 682, 703
BstXI CCANNNNNNTGG 1 cut(s) 671
BstYI RGATCY 2 cut(s) 682, 703
BsuRI GGCC 5 cut(s) 57, 194, 233, 904, 980
BtgI CCRYGG 2 cut(s) 447, 830
BtgZI GCGATG 1 cut(s) 27
BtsCI GGATG 4 cut(s) 184, 371, 467, 1074
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 5 cut(s) 171, 235, 260, 341, 931
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 813
Cfr13I GGNCC 3 cut(s) 231, 232, 902
CseI GACGC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 351, 587, 845
CviAII CATG 7 cut(s) 523, 632, 671, 772, 831, 889, 893
CviQI GTAC 3 cut(s) 351, 587, 845
DdeI CTNAG 1 cut(s) 203
DpnI GATC 7 cut(s) 29, 115, 315, 320, 458, 684, 705
DpnII GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
DraIII CACNNNGTG 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 978
Eam1104I CTCTTC 3 cut(s) 12, 81, 420
EarI CTCTTC 3 cut(s) 12, 81, 420
EciI GGCGGA 2 cut(s) 690, 816
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 3 cut(s) 174, 361, 830
Eco24I GRGCYC 2 cut(s) 235, 714
Eco32I GATATC 1 cut(s) 821
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 741
Eco72I CACGTG 1 cut(s) 808
Eco88I CYCGRG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 2 cut(s) 231, 902
EcoRV GATATC 1 cut(s) 821
EcoT14I CCWWGG 3 cut(s) 174, 361, 830
EcoT22I ATGCAT 1 cut(s) 894
EcoT38I GRGCYC 2 cut(s) 235, 714
ErhI CCWWGG 3 cut(s) 174, 361, 830
Esp3I CGTCTC 1 cut(s) 661
FaeI CATG 7 cut(s) 526, 635, 674, 775, 834, 892, 896
FatI CATG 7 cut(s) 522, 631, 670, 771, 830, 888, 892
FauI CCCGC 2 cut(s) 242, 979
FauNDI CATATG 1 cut(s) 277
FbaI TGATCA 1 cut(s) 318
FblI GTMKAC 2 cut(s) 645, 768
Fnu4HI GCNGC 2 cut(s) 55, 1115
FokI GGATG 4 cut(s) 191, 378, 474, 1081
FriOI GRGCYC 2 cut(s) 235, 714
Fsp4HI GCNGC 2 cut(s) 55, 1115
FspBI CTAG 3 cut(s) 329, 791, 957
GlaI GCGC 1 cut(s) 812
GluI GCNGC 2 cut(s) 55, 1115
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 5 cut(s) 57, 194, 233, 904, 980
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 950
HhaI GCGC 1 cut(s) 813
Hin1I GRCGYC 2 cut(s) 942, 1213
Hin1II CATG 7 cut(s) 526, 635, 674, 775, 834, 892, 896
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 5 cut(s) 48, 119, 392, 794, 1124
HpaI GTTAAC 1 cut(s) 399
HpaII CCGG 1 cut(s) 135
HphI GGTGA 3 cut(s) 611, 653, 1090
Hpy166II GTNNAC 7 cut(s) 93, 399, 553, 646, 769, 788, 1211
Hpy188I TCNGA 6 cut(s) 118, 206, 318, 461, 532, 760
Hpy188III TCNNGA 3 cut(s) 632, 707, 1159
Hpy8I GTNNAC 7 cut(s) 93, 399, 553, 646, 769, 788, 1211
Hpy99I CGWCG 1 cut(s) 691
HpyAV CCTTC 4 cut(s) 685, 765, 1002, 1059
HpyCH4III ACNGT 3 cut(s) 451, 550, 874
HpyCH4IV ACGT 3 cut(s) 127, 807, 1213
HpyCH4V TGCA 4 cut(s) 488, 670, 892, 1117
HpyF10VI GCNNNNNNNGC 3 cut(s) 366, 884, 930
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 3 cut(s) 127, 807, 1213
Hsp92I GRCGYC 2 cut(s) 942, 1213
Hsp92II CATG 7 cut(s) 526, 635, 674, 775, 834, 892, 896
HspAI GCGC 1 cut(s) 811
KpnI GGTACC 1 cut(s) 354
Ksp22I TGATCA 1 cut(s) 318
KspAI GTTAAC 1 cut(s) 399
Kzo9I GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 2 cut(s) 929, 973
Lsp1109I GCAGC 1 cut(s) 1101
LweI GCATC 6 cut(s) 287, 356, 874, 942, 1071, 1125
MaeI CTAG 3 cut(s) 329, 791, 957
MaeII ACGT 3 cut(s) 127, 807, 1213
MaeIII GTNAC 3 cut(s) 599, 868, 1190
MalI GATC 7 cut(s) 29, 115, 315, 320, 458, 684, 705
MboI GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
MboII GAAGA 5 cut(s) 29, 68, 407, 630, 950
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 2 cut(s) 682, 703
MhlI GDGCHC 2 cut(s) 235, 714
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 8 cut(s) 568, 612, 722, 749, 850, 985, 1142, 1185
MluNI TGGCCA 1 cut(s) 980
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 3 cut(s) 87, 369, 434
Mox20I TGGCCA 1 cut(s) 980
Mph1103I ATGCAT 1 cut(s) 894
MscI TGGCCA 1 cut(s) 980
MseI TTAA 6 cut(s) 384, 398, 594, 1140, 1154, 1219
MslI CAYNNNNRTG 2 cut(s) 452, 893
Msp20I TGGCCA 1 cut(s) 980
MspI CCGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 612
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 366, 884, 930
NcoI CCATGG 1 cut(s) 830
NdeI CATATG 1 cut(s) 277
NdeII GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
NlaIII CATG 7 cut(s) 526, 635, 674, 775, 834, 892, 896
NlaIV GGNNCC 7 cut(s) 232, 233, 352, 705, 903, 925, 969
NmuCI GTSAC 3 cut(s) 599, 868, 1190
NsiI ATGCAT 1 cut(s) 894
NspI RCATGY 1 cut(s) 896
NspV TTCGAA 1 cut(s) 856
OliI CACNNNNGTG 1 cut(s) 452
PaeR7I CTCGAG 1 cut(s) 743
PagI TCATGA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 548
PciSI GCTCTTC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 1195
PfeI GAWTC 4 cut(s) 48, 119, 794, 1124
PkrI GCNGC 2 cut(s) 56, 1116
PleI GAGTC 1 cut(s) 386
PmaCI CACGTG 1 cut(s) 808
PmlI CACGTG 1 cut(s) 808
PpsI GAGTC 1 cut(s) 386
Ppu21I YACGTR 2 cut(s) 128, 808
Psp124BI GAGCTC 1 cut(s) 714
PspCI CACGTG 1 cut(s) 808
PspN4I GGNNCC 7 cut(s) 232, 233, 352, 705, 903, 925, 969
PspOMI GGGCCC 1 cut(s) 231
PspPI GGNCC 3 cut(s) 231, 232, 902
PspXI VCTCGAGB 1 cut(s) 743
PsuI RGATCY 2 cut(s) 682, 703
RsaI GTAC 3 cut(s) 352, 588, 846
RsaNI GTAC 3 cut(s) 351, 587, 845
RseI CAYNNNNRTG 2 cut(s) 452, 893
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 6 cut(s) 384, 398, 594, 1140, 1154, 1219
SatI GCNGC 2 cut(s) 55, 1115
Sau3AI GATC 7 cut(s) 27, 113, 313, 318, 456, 682, 703
Sau96I GGNCC 3 cut(s) 231, 232, 902
SchI GAGTC 1 cut(s) 386
SduI GDGCHC 2 cut(s) 235, 714
SfaNI GCATC 6 cut(s) 287, 356, 874, 942, 1071, 1125
Sfr274I CTCGAG 1 cut(s) 743
SfuI TTCGAA 1 cut(s) 856
SlaI CTCGAG 1 cut(s) 743
SmiMI CAYNNNNRTG 2 cut(s) 452, 893
SmlI CTYRAG 2 cut(s) 743, 814
SmoI CTYRAG 2 cut(s) 743, 814
Sse9I AATT 8 cut(s) 568, 612, 722, 749, 850, 985, 1142, 1185
SsiI CCGC 7 cut(s) 54, 235, 287, 675, 827, 927, 972
SspMI CTAG 3 cut(s) 329, 791, 957
SstI GAGCTC 1 cut(s) 714
StyI CCWWGG 3 cut(s) 174, 361, 830
TaaI ACNGT 3 cut(s) 451, 550, 874
TaiI ACGT 3 cut(s) 130, 810, 1216
TaqI TCGA 8 cut(s) 689, 718, 744, 856, 936, 1044, 1133, 1198
TaqII GACCGA 1 cut(s) 902
TasI AATT 8 cut(s) 568, 612, 722, 749, 850, 985, 1142, 1185
TatI WGTACW 2 cut(s) 586, 844
TauI GCSGC 1 cut(s) 57
TfiI GAWTC 4 cut(s) 48, 119, 794, 1124
Tru1I TTAA 6 cut(s) 384, 398, 594, 1140, 1154, 1219
Tru9I TTAA 6 cut(s) 384, 398, 594, 1140, 1154, 1219
TseFI GTSAC 3 cut(s) 599, 868, 1190
TseI GCWGC 1 cut(s) 1114
Tsp45I GTSAC 3 cut(s) 599, 868, 1190
TspDTI ATGAA 6 cut(s) 30, 68, 484, 511, 620, 898
TspGWI ACGGA 3 cut(s) 322, 444, 675
XapI RAATTY 3 cut(s) 749, 850, 1142
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 895
XhoI CTCGAG 1 cut(s) 743
XmiI GTMKAC 2 cut(s) 645, 768
XspI CTAG 3 cut(s) 329, 791, 957
ZraI GACGTC 1 cut(s) 1214
Zsp2I ATGCAT 1 cut(s) 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.