MD07G1130200.v1.1

Programmed cell death protein 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004138
Physical Location & Seq
Forward (+)
18573634 .. 18576446
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1130200.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGAACCCAATTCCGTACCAACCGCAGGCGCCCCACCCTCCGTGGCCTCCGGTGGTGCCGCCCAACCCGCCAATGTCGAGCTCCTTCTGGGAAACCACGAACGTCCGTGATCGGCTCAAGAACTTGCAGGACACTCTGAGTCTCGCAAAAGCAATGCAGAAAGAGCTTCAGATGCTGCAATTGATCAAAGACGGTAAAGGGTCCGACGATGGTGAAGCTTCCGTTGGTGGGTTTTCGGAGTATTTGAAAGAAAGGAGGGTAAATGTGGAGTCTCAAGTGACGCTCTCACTGGAAGCTGCGAATGCTTTGATGGCGAAACTGCAAGTTGAGCTGGAACCGTTTAGAATGATCACGCATGAAATGTGTCCGTGGGAGGAGAAGTCTGCGGTGGTGCGGTTAGCAAATAAGATGCGCAAGACGAAGAGGAATAAACGGTGGAGGAAAGCGAAGAGGAAGCGCATTGCGGAAATGATTGCAGAGGAGCGTGAGAGGTTCGAGGAAGCTGATCGAGAAGCTGATGAGTGGAGAGCTAGGGAAATTGCCAAAGATATTGCCAAACGCAAGATGGAGGATATGAAGAAAATTGCAAAGCTTAAAGCAAAAGAAGAGAGAAAGAGACTAGAATCCGAGCTTGAGACGGTATTGATTGTAGAGAAATTGCAACAGTTGCGTTCCATCAGGATCGAAAAATTGAAGAAACAAGGTCATTTTCTCCCAGAGGAGGATGATAAATTTCTTGAAAAAGTACGGGCTGCAGTTGAGGAAGAGGAGCAACAAGTTATCTTGGCAGCTGATATGGACGCTGCTAAGGATGCCATAGCAACTGTTGAGCAGTCCAGGAAAATAACCGAGAATGTCAGACCTGACTCCAAGGATGAAAGCAGCGATAGAGACGAGACAAAGGAGAGTAAAGACCAGACAACTCCAAGCACTAATGCAGGGACCTCTACTTCAGTCACTAACAAGGATTACGGGAAACAAGTTTCGCAGGGAGAAGGACATAGCGGAGCTTATGATGCTGTGGTAAACTTACCTATTGAATTTTATCACTACTATCATGGCAGCAACACCGATATGGGCACGCTTATTGAGGTGAGAAGAACATGGGATGCCTATATAAGACCAGGAGGAAGCCGCATACCAGGACATTGGGTTCAACCCCCGCCACCTGCAGATGATATATGGGCATCCTACCTGGTGCAGCCTAAATGA

Protein Analysis

404

Amino Acids

46.25

Weight (kDa)

6.72

Isoelectric Point (pI)

56.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDCD7 PF16021 119 - 399 1.3e-14 Programmed cell death protein 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1177
Acc16I TGCGCA 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 1177
AccB1I GGYRCC 2 cut(s) 28, 55
AciI CCGC 9 cut(s) 23, 59, 68, 386, 394, 464, 1005, 1135, 1163
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 2 cut(s) 731, 1040
AcuI CTGAAG 2 cut(s) 152, 936
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 2 cut(s) 17, 747
AfiI CCNNNNNNNGG 3 cut(s) 25, 228, 721
AgsI TTSAA 5 cut(s) 247, 694, 740, 1040, 1157
AjnI CCWGG 4 cut(s) 836, 1123, 1141, 1194
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AloI GAACNNNNNNTCC 2 cut(s) 1137, 1169
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 6 cut(s) 146, 276, 610, 629, 885, 890
AlwI GGATC 1 cut(s) 689
AlwNI CAGNNNCTG 1 cut(s) 175
AoxI GGCC 1 cut(s) 44
ApeKI GCWGC 8 cut(s) 175, 296, 752, 788, 803, 882, 1062, 1201
ApoI RAATTY 2 cut(s) 731, 1040
AspLEI GCGC 3 cut(s) 31, 414, 459
AspS9I GGNCC 2 cut(s) 201, 942
AsuHPI GGTGA 2 cut(s) 224, 1105
AvaII GGWCC 2 cut(s) 201, 942
BaeGI GKGCMC 1 cut(s) 1082
BanI GGYRCC 2 cut(s) 28, 55
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 7 cut(s) 162, 283, 739, 790, 800, 894, 1074
BccI CCATC 4 cut(s) 203, 304, 559, 683
BciT130I CCWGG 4 cut(s) 838, 1125, 1143, 1196
BclI TGATCA 2 cut(s) 183, 348
BcoDI GTCTC 6 cut(s) 146, 276, 610, 629, 885, 890
BfaI CTAG 2 cut(s) 531, 620
BfmI CTRYAG 2 cut(s) 753, 1170
BfoI RGCGCY 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 1177
Bme1390I CCNGG 4 cut(s) 838, 1125, 1143, 1196
Bme18I GGWCC 2 cut(s) 201, 942
BmgT120I GGNCC 2 cut(s) 201, 942
BmiI GGNNCC 5 cut(s) 30, 57, 202, 336, 943
BmrFI CCNGG 4 cut(s) 838, 1125, 1143, 1196
BmsI GCATC 6 cut(s) 162, 399, 802, 1006, 1099, 1196
Bpu10I CCTNAGC 1 cut(s) 807
BpuEI CTTGAG 3 cut(s) 101, 258, 653
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 41, 368, 870
BsaWI WCCGGW 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 228, 721
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 2 cut(s) 159, 459
BseBI CCWGG 4 cut(s) 838, 1125, 1143, 1196
BseDI CCNNGG 3 cut(s) 41, 368, 870
BseGI GGATG 5 cut(s) 730, 817, 880, 1114, 1187
BseLI CCNNNNNNNGG 3 cut(s) 25, 228, 721
BseMI GCAATG 2 cut(s) 159, 459
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 4 cut(s) 389, 494, 734, 782
BseSI GKGCMC 1 cut(s) 1082
BseXI GCAGC 7 cut(s) 162, 283, 739, 790, 800, 894, 1074
BshFI GGCC 1 cut(s) 46
BshNI GGYRCC 2 cut(s) 28, 55
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 955
BslI CCNNNNNNNGG 3 cut(s) 25, 228, 721
BsmAI GTCTC 6 cut(s) 146, 276, 610, 629, 885, 890
BsmBI CGTCTC 2 cut(s) 629, 885
BsmFI GGGAC 1 cut(s) 955
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 83, 1082
Bsp143I GATC 5 cut(s) 109, 183, 348, 505, 681
BspACI CCGC 9 cut(s) 23, 59, 68, 386, 394, 464, 1005, 1135, 1163
BspANI GGCC 1 cut(s) 46
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 5 cut(s) 30, 57, 202, 336, 943
BspMAI CTGCAG 2 cut(s) 757, 1174
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 1 cut(s) 689
BspT107I GGYRCC 2 cut(s) 28, 55
BsrDI GCAATG 2 cut(s) 159, 459
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 3 cut(s) 41, 368, 870
BssMI GATC 5 cut(s) 109, 183, 348, 505, 681
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 870
Bst2UI CCWGG 4 cut(s) 838, 1125, 1143, 1196
Bst4CI ACNGT 6 cut(s) 194, 339, 435, 640, 666, 826
Bst6I CTCTTC 4 cut(s) 416, 443, 600, 759
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 667
BstC8I GCNNGC 2 cut(s) 27, 1082
BstDEI CTNAG 2 cut(s) 137, 807
BstDSI CCRYGG 2 cut(s) 41, 368
BstF5I GGATG 5 cut(s) 730, 817, 880, 1114, 1187
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 3 cut(s) 31, 414, 459
BstKTI GATC 5 cut(s) 112, 186, 351, 508, 684
BstMAI GTCTC 6 cut(s) 146, 276, 610, 629, 885, 890
BstMBI GATC 5 cut(s) 109, 183, 348, 505, 681
BstMWI GCNNNNNNNGC 9 cut(s) 67, 163, 172, 302, 311, 328, 667, 812, 1016
BstNI CCWGG 4 cut(s) 838, 1125, 1143, 1196
BstSCI CCNGG 4 cut(s) 836, 1123, 1141, 1194
BstSFI CTRYAG 2 cut(s) 753, 1170
BstSLI GKGCMC 1 cut(s) 1082
BstV1I GCAGC 7 cut(s) 162, 283, 739, 790, 800, 894, 1074
BstXI CCANNNNNNTGG 1 cut(s) 1149
BsuRI GGCC 1 cut(s) 46
BtgI CCRYGG 2 cut(s) 41, 368
BtsCI GGATG 5 cut(s) 730, 817, 880, 1114, 1187
BtsIMutI CAGTG 1 cut(s) 287
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 2 cut(s) 27, 1082
CaiI CAGNNNCTG 1 cut(s) 175
CfoI GCGC 3 cut(s) 31, 414, 459
Cfr13I GGNCC 2 cut(s) 201, 942
CseI GACGC 2 cut(s) 289, 809
CsiI ACCWGGT 1 cut(s) 1194
Csp6I GTAC 2 cut(s) 16, 746
CviAII CATG 3 cut(s) 356, 1058, 1104
CviQI GTAC 2 cut(s) 16, 746
DdeI CTNAG 2 cut(s) 137, 807
DinI GGCGCC 1 cut(s) 30
DpnI GATC 5 cut(s) 111, 185, 350, 507, 683
DpnII GATC 5 cut(s) 109, 183, 348, 505, 681
Eam1104I CTCTTC 4 cut(s) 416, 443, 600, 759
EarI CTCTTC 4 cut(s) 416, 443, 600, 759
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 870
Eco24I GRGCYC 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 201, 942
Eco53kI GAGCTC 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 152, 936
EcoICRI GAGCTC 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 942
EcoRII CCWGG 4 cut(s) 836, 1123, 1141, 1194
EcoT14I CCWWGG 1 cut(s) 870
EcoT38I GRGCYC 1 cut(s) 83
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 870
Esp3I CGTCTC 2 cut(s) 629, 885
FaeI CATG 3 cut(s) 359, 1061, 1107
FaqI GGGAC 1 cut(s) 955
FatI CATG 3 cut(s) 355, 1057, 1103
FauI CCCGC 2 cut(s) 75, 1170
FbaI TGATCA 2 cut(s) 183, 348
FokI GGATG 5 cut(s) 737, 824, 887, 1121, 1174
FriOI GRGCYC 1 cut(s) 83
FspBI CTAG 2 cut(s) 531, 620
FspI TGCGCA 1 cut(s) 413
GlaI GCGC 3 cut(s) 30, 413, 458
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 1 cut(s) 46
HapII CCGG 1 cut(s) 50
HgaI GACGC 2 cut(s) 289, 809
HhaI GCGC 3 cut(s) 31, 414, 459
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 3 cut(s) 359, 1061, 1107
Hin6I GCGC 3 cut(s) 29, 412, 457
HinP1I GCGC 3 cut(s) 29, 412, 457
HindIII AAGCTT 2 cut(s) 216, 590
HinfI GANTC 4 cut(s) 139, 269, 623, 866
HpaII CCGG 1 cut(s) 50
HphI GGTGA 2 cut(s) 224, 1105
Hpy166II GTNNAC 1 cut(s) 1027
Hpy188I TCNGA 6 cut(s) 138, 171, 205, 238, 628, 860
Hpy188III TCNNGA 4 cut(s) 118, 509, 679, 737
Hpy8I GTNNAC 1 cut(s) 1027
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 94, 989
HpyCH4III ACNGT 6 cut(s) 194, 339, 435, 640, 666, 826
HpyCH4IV ACGT 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 163, 172, 302, 311, 328, 667, 812, 1016
HpyF3I CTNAG 2 cut(s) 137, 807
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 3 cut(s) 359, 1061, 1107
HspAI GCGC 3 cut(s) 29, 412, 457
KasI GGCGCC 1 cut(s) 28
Ksp22I TGATCA 2 cut(s) 183, 348
Kzo9I GATC 5 cut(s) 109, 183, 348, 505, 681
LmnI GCTCC 4 cut(s) 86, 481, 769, 1007
Lsp1109I GCAGC 7 cut(s) 162, 283, 739, 790, 800, 894, 1074
LweI GCATC 6 cut(s) 162, 399, 802, 1006, 1099, 1196
MabI ACCWGGT 1 cut(s) 1194
MaeI CTAG 2 cut(s) 531, 620
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 277, 955
MalI GATC 5 cut(s) 111, 185, 350, 507, 683
MboI GATC 5 cut(s) 109, 183, 348, 505, 681
MboII GAAGA 7 cut(s) 433, 460, 589, 617, 706, 776, 1110
MfeI CAATTG 1 cut(s) 179
MhlI GDGCHC 2 cut(s) 83, 1082
MluCI AATT 8 cut(s) 9, 179, 537, 582, 656, 689, 731, 1040
Mly113I GGCGCC 1 cut(s) 29
MlyI GAGTC 3 cut(s) 148, 278, 860
MmeI TCCRAC 1 cut(s) 228
MseI TTAA 1 cut(s) 594
MslI CAYNNNNRTG 1 cut(s) 1073
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 4 cut(s) 838, 1125, 1143, 1196
MunI CAATTG 1 cut(s) 179
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 4 cut(s) 838, 1125, 1143, 1196
MwoI GCNNNNNNNGC 9 cut(s) 67, 163, 172, 302, 311, 328, 667, 812, 1016
NarI GGCGCC 1 cut(s) 29
NdeII GATC 5 cut(s) 109, 183, 348, 505, 681
NlaIII CATG 3 cut(s) 359, 1061, 1107
NlaIV GGNNCC 5 cut(s) 30, 57, 202, 336, 943
NmuCI GTSAC 2 cut(s) 277, 955
NsbI TGCGCA 1 cut(s) 413
PaqCI CACCTGC 1 cut(s) 1177
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 836
PleI GAGTC 3 cut(s) 147, 277, 860
PluTI GGCGCC 1 cut(s) 32
PpsI GAGTC 3 cut(s) 147, 277, 860
PpuMI RGGWCCY 1 cut(s) 942
Psp124BI GAGCTC 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 942
Psp6I CCWGG 4 cut(s) 836, 1123, 1141, 1194
PspGI CCWGG 4 cut(s) 836, 1123, 1141, 1194
PspN4I GGNNCC 5 cut(s) 30, 57, 202, 336, 943
PspPI GGNCC 2 cut(s) 201, 942
PspPPI RGGWCCY 1 cut(s) 942
PstI CTGCAG 2 cut(s) 757, 1174
PstNI CAGNNNCTG 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 2 cut(s) 17, 747
RsaNI GTAC 2 cut(s) 16, 746
RseI CAYNNNNRTG 1 cut(s) 1073
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 1 cut(s) 594
Sau3AI GATC 5 cut(s) 109, 183, 348, 505, 681
Sau96I GGNCC 2 cut(s) 201, 942
SchI GAGTC 3 cut(s) 148, 278, 860
ScrFI CCNGG 4 cut(s) 838, 1125, 1143, 1196
SduI GDGCHC 2 cut(s) 83, 1082
SexAI ACCWGGT 1 cut(s) 1194
SfaNI GCATC 6 cut(s) 162, 399, 802, 1006, 1099, 1196
SfcI CTRYAG 2 cut(s) 753, 1170
SfoI GGCGCC 1 cut(s) 30
SinI GGWCC 2 cut(s) 201, 942
SmiMI CAYNNNNRTG 1 cut(s) 1073
SmlI CTYRAG 3 cut(s) 116, 273, 632
SmoI CTYRAG 3 cut(s) 116, 273, 632
Sse9I AATT 8 cut(s) 9, 179, 537, 582, 656, 689, 731, 1040
SsiI CCGC 9 cut(s) 23, 59, 68, 386, 394, 464, 1005, 1135, 1163
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 2 cut(s) 531, 620
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 4 cut(s) 836, 1123, 1141, 1194
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 6 cut(s) 194, 339, 435, 640, 666, 826
TaiI ACGT 1 cut(s) 105
TaqI TCGA 4 cut(s) 77, 495, 508, 684
TasI AATT 8 cut(s) 9, 179, 537, 582, 656, 689, 731, 1040
TauI GCSGC 2 cut(s) 61, 1137
TfiI GAWTC 1 cut(s) 623
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 2 cut(s) 277, 955
TseI GCWGC 8 cut(s) 175, 296, 752, 788, 803, 882, 1062, 1201
Tsp45I GTSAC 2 cut(s) 277, 955
TspDTI ATGAA 4 cut(s) 17, 372, 590, 891
TspGWI ACGGA 4 cut(s) 30, 95, 211, 357
TspRI CASTG 1 cut(s) 294
VpaK11BI GGWCC 2 cut(s) 201, 942
XapI RAATTY 2 cut(s) 731, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XspI CTAG 2 cut(s) 531, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.