Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002832

Physical Location & Seq

Genomic Coordinates Forward (+)

2087939 .. 2090678

Gene Structure 2740 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1028600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1044 bp

ATGGGATCGAAGTCTGAATCCGTTTGTGTCACTGGCGCGTCCGGTTTCATCGGCTCCTGGCTTGTCATGAGACTCCTCGAGCGCGGTTACACCGTCCGCGCCACCGTGCGTGACCCCACGAATCAGAAGAAGGTGAAGCATCTGTTGGACTTGCCGAAGGCGGAGACGCACCTGACGCTGTGGAAGGCGGACCTGGCGGACGAGGGAAGCTTTGATGAAGCCATACAAGGCTGCACCGGAGTATTCCATGTTGCTACACCTATGGACTTTGAGTCCAAGGACCCCGAGAATGAAGTGATCAAGCCAACAATCAATGGACTTTTGGACATCCTGAAGGCATGCCAGAAAGTTAAAACAGTTCGAAGGTTGGTCTTCACATCCTCAGCAGGAACCGTTATTATGGAAGAGCACAAAAAGCCGGTTTACGACGAATCCAACTGGAGCGACGTTGAATTTTGCCTCTCCGTCAAGATGACTGGTTGGATGTACTTCGTTTCCAAGACTCTAGCTGAGCAAGCTGCATGGAAGTATGCCAAAGAAAACAACATTGATTTCATCACCATTATCCCAACTCTTGTGATTGGTCCATTTCTCATGCCATCCATGCCACCAAGCCTCATCACTGGACTTTCACCAATCACACGAACTAAATCACATTATGGCATCATCAAGCAGGGGCAGTATGTTCACTTGGACGACCTCTGCAATTCTCACATCTATTTGTATGAGCATCCGAAAGCTGAGGGCCGTTACATTTGTTCGTCACATGATGCAACAATTCACGACCTTGTAAAAATGCTCAGAGAAAAATACCCTGCGTACAATATTCCCACAAAGTTCGAGGGCATTGACGACAACTTAGATACTATTCATTTTTCTTCAAAGAAGTTGAGGGACATAGGGTTTGAGTTCAAGTACAGCTTCGAGGACATGTTTGTGGGGGCTGTTGATACGTGCCGGGCAAAGGGATTGATTCCGATTCCGGCCGAGAAAACTGAGGCTGCTGATGAGAGCAACGTCGTCGACATCAAAATTGCCGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0008150 GO:0008152 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0012505 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0019898 GO:0031090 GO:0031312 GO:0031984 GO:0042175 GO:0042406 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045552 GO:0046148 GO:0046283 GO:0055114 GO:0071704 GO:0098827 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00941 ko01100 ko01110 map00941 map01100 map01110

Pfam Domains

Protein Families

Epimerase

Protein Analysis

348

Amino Acids

38.94

Weight (kDa)

6.02

Isoelectric Point (pI)

31.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NmrA	PF05368	6 - 130	9.6e-12	NmrA-like family
Epimerase	PF01370	8 - 248	5.3e-28	NAD dependent epimerase/dehydratase family
KR	PF08659	8 - 134	7.3e-06	KR domain
3Beta_HSD	PF01073	10 - 176	2e-19	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	10 - 266	2.4e-17	GDP-mannose 4,6 dehydratase
NAD_binding_4	PF07993	10 - 202	1.3e-13	Male sterility protein
NAD_binding_10	PF13460	12 - 132	7e-13	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42800
fragaria_vesca	FvH4_2g39520 FvH4_2g39520 FvH4_2g39520 FvH4_2g39520 FvH4_2g39520
malus_domestica	MD08G1028200.v1.1 MD08G1028400.v1.1 MD08G1028600.v1.1 MD15G1024100.v1.1
prunus_persica	Prupe.1G376400_v2.0.a1 Prupe.1G376400_v2.0.a1
pyrus_communis	pycom08g02250 pycom15g02070
rosa_chinensis	RchiOBHm_Chr6g0301421
rosa_laevigata	RLG00000011254 RLG00000011255
rosa_multiflora	Rmu_sc0003058.1_g000015 Rmu_sc0003058.1_g000017 Rmu_sc0014417.1_g000009 Rmu_sc0040371.1_g000001
rosa_roxburghii	Rroxscaffold_171G00441500 Rroxscaffold_7G00166830 Rroxscaffold_7G00172560
rosa_rugosa	Rorug06G0250100 Rorug06G0304400.1 Rorug06G0304500
rosa_samantha	Rh1BG361700 Rh6AG363400 Rh6AG418200 Rh6AG418300 Rh6BG371500 Rh6BG424500 Rh6CG378000 Rh6CG431700 Rh6DG417500 Rh6DG417600
rosa_wichuraiana	Rw6G031710 Rw6G036190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1023
AccII	CGCG	3 cut(s)	38, 84, 99
AciI	CCGC	6 cut(s)	84, 97, 161, 188, 197, 1038
AclWI	GGATC	1 cut(s)	13
AcoI	YGGCCR	1 cut(s)	984
AcsI	RAATTY	1 cut(s)	452
AcuI	CTGAAG	1 cut(s)	353
AfaI	GTAC	3 cut(s)	488, 821, 917
AfiI	CCNNNNNNNGG	1 cut(s)	964
AflIII	ACRYGT	1 cut(s)	930
AgsI	TTSAA	3 cut(s)	452, 882, 913
AhdI	GACNNNNNGTC	1 cut(s)	271
AjnI	CCWGG	2 cut(s)	56, 192
AjuI	GAANNNNNNNTTGG	2 cut(s)	128, 160
AluBI	AGCT	5 cut(s)	210, 509, 518, 740, 921
AluI	AGCT	5 cut(s)	210, 509, 518, 740, 921
Alw21I	GWGCWC	1 cut(s)	411
Alw26I	GTCTC	2 cut(s)	64, 158
AlwI	GGATC	1 cut(s)	13
Ama87I	CYCGRG	2 cut(s)	77, 284
AoxI	GGCC	2 cut(s)	745, 984
ApeKI	GCWGC	3 cut(s)	231, 518, 1001
ApoI	RAATTY	1 cut(s)	452
ArsI	GACNNNNNNTTYG	2 cut(s)	887, 919
AspLEI	GCGC	3 cut(s)	38, 84, 101
AspS9I	GGNCC	4 cut(s)	190, 280, 584, 745
AsuC2I	CCSGG	1 cut(s)	959
AsuHPI	GGTGA	3 cut(s)	145, 550, 624
AsuII	TTCGAA	1 cut(s)	361
AvaI	CYCGRG	2 cut(s)	77, 284
AvaII	GGWCC	3 cut(s)	190, 280, 584
BbsI	GAAGAC	1 cut(s)	364
Bbv12I	GWGCWC	1 cut(s)	411
BbvCI	CCTCAGC	2 cut(s)	382, 741
BbvI	GCAGC	3 cut(s)	218, 505, 988
BccI	CCATC	1 cut(s)	607
BceAI	ACGGC	1 cut(s)	732
BcgI	CGANNNNNNTGC	2 cut(s)	1003, 1037
BciT130I	CCWGG	2 cut(s)	58, 194
BclI	TGATCA	1 cut(s)	297
BcnI	CCSGG	1 cut(s)	959
BcoDI	GTCTC	2 cut(s)	64, 158
BfaI	CTAG	1 cut(s)	506
BisI	GCNGC	4 cut(s)	232, 519, 1002, 1038
BlpI	GCTNAGC	1 cut(s)	510
BlsI	GCNGC	4 cut(s)	233, 520, 1003, 1039
Bme1390I	CCNGG	3 cut(s)	58, 194, 959
Bme18I	GGWCC	3 cut(s)	190, 280, 584
BmeRI	GACNNNNNGTC	1 cut(s)	271
BmeT110I	CYCGRG	2 cut(s)	77, 284
BmgT120I	GGNCC	4 cut(s)	190, 280, 584, 745
BmiI	GGNNCC	3 cut(s)	55, 282, 391
BmrFI	CCNGG	3 cut(s)	58, 194, 959
BmsI	GCATC	4 cut(s)	148, 672, 739, 760
BpiI	GAAGAC	1 cut(s)	364
BpmI	CTGGAG	1 cut(s)	460
Bpu10I	CCTNAGC	2 cut(s)	382, 741
Bpu1102I	GCTNAGC	1 cut(s)	510
Bpu14I	TTCGAA	1 cut(s)	361
BpuMI	CCSGG	1 cut(s)	959
BsaAI	YACGTR	1 cut(s)	954
BsaJI	CCNNGG	1 cut(s)	276
BsaWI	WCCGGW	2 cut(s)	41, 236
Bsc4I	CCNNNNNNNGG	1 cut(s)	964
Bse118I	RCCGGY	1 cut(s)	418
Bse1I	ACTGG	4 cut(s)	37, 443, 481, 628
BseBI	CCWGG	2 cut(s)	58, 194
BseDI	CCNNGG	1 cut(s)	276
BseGI	GGATG	5 cut(s)	327, 377, 489, 599, 730
BseLI	CCNNNNNNNGG	1 cut(s)	964
BseMII	CTCAG	5 cut(s)	396, 501, 732, 814, 987
BseNI	ACTGG	4 cut(s)	37, 443, 481, 628
BseRI	GAGGAG	1 cut(s)	65
BseX3I	CGGCCG	1 cut(s)	984
BseXI	GCAGC	3 cut(s)	218, 505, 988
BsgI	GTGCAG	1 cut(s)	217
Bsh1236I	CGCG	3 cut(s)	38, 84, 99
Bsh1285I	CGRYCG	1 cut(s)	987
BshFI	GGCC	2 cut(s)	747, 986
BsiEI	CGRYCG	1 cut(s)	987
BsiHKAI	GWGCWC	1 cut(s)	411
BsiHKCI	CYCGRG	2 cut(s)	77, 284
BsiSI	CCGG	5 cut(s)	42, 237, 419, 958, 983
BslFI	GGGAC	1 cut(s)	908
BslI	CCNNNNNNNGG	1 cut(s)	964
BsmAI	GTCTC	2 cut(s)	64, 158
BsmBI	CGTCTC	1 cut(s)	158
BsmFI	GGGAC	1 cut(s)	908
BsnI	GGCC	2 cut(s)	747, 986
BsoBI	CYCGRG	2 cut(s)	77, 284
Bsp119I	TTCGAA	1 cut(s)	361
Bsp1286I	GDGCHC	1 cut(s)	411
Bsp143I	GATC	2 cut(s)	5, 297
Bsp1720I	GCTNAGC	1 cut(s)	510
BspACI	CCGC	6 cut(s)	84, 97, 161, 188, 197, 1038
BspANI	GGCC	2 cut(s)	747, 986
BspCNI	CTCAG	5 cut(s)	395, 502, 733, 813, 988
BspFNI	CGCG	3 cut(s)	38, 84, 99
BspHI	TCATGA	1 cut(s)	66
BspLI	GGNNCC	3 cut(s)	55, 282, 391
BspPI	GGATC	1 cut(s)	13
BspQI	GCTCTTC	1 cut(s)	399
BspT104I	TTCGAA	1 cut(s)	361
BsrFI	RCCGGY	1 cut(s)	418
BsrI	ACTGG	4 cut(s)	37, 443, 481, 628
BssAI	RCCGGY	1 cut(s)	418
BssECI	CCNNGG	1 cut(s)	276
BssMI	GATC	2 cut(s)	5, 297
BssT1I	CCWWGG	1 cut(s)	276
Bst2UI	CCWGG	2 cut(s)	58, 194
Bst4CI	ACNGT	4 cut(s)	94, 106, 358, 394
Bst6I	CTCTTC	1 cut(s)	399
BstBAI	YACGTR	1 cut(s)	954
BstBI	TTCGAA	1 cut(s)	361
BstC8I	GCNNGC	2 cut(s)	340, 516
BstDEI	CTNAG	6 cut(s)	382, 510, 741, 800, 859, 996
BstF5I	GGATG	5 cut(s)	327, 377, 489, 599, 730
BstFNI	CGCG	3 cut(s)	38, 84, 99
BstHHI	GCGC	3 cut(s)	38, 84, 101
BstKTI	GATC	2 cut(s)	8, 300
BstMAI	GTCTC	2 cut(s)	64, 158
BstMBI	GATC	2 cut(s)	5, 297
BstMCI	CGRYCG	1 cut(s)	987
BstMWI	GCNNNNNNNGC	5 cut(s)	175, 194, 415, 515, 604
BstNI	CCWGG	2 cut(s)	58, 194
BstNSI	RCATGY	2 cut(s)	342, 934
BstSCI	CCNGG	3 cut(s)	56, 192, 957
BstUI	CGCG	3 cut(s)	38, 84, 99
BstV1I	GCAGC	3 cut(s)	218, 505, 988
BstV2I	GAAGAC	1 cut(s)	364
BstZI	CGGCCG	1 cut(s)	984
BsuRI	GGCC	2 cut(s)	747, 986
BtsCI	GGATG	5 cut(s)	327, 377, 489, 599, 730
BtsIMutI	CAGTG	2 cut(s)	30, 621
Cac8I	GCNNGC	2 cut(s)	340, 516
CciI	TCATGA	1 cut(s)	66
CfoI	GCGC	3 cut(s)	38, 84, 101
Cfr10I	RCCGGY	1 cut(s)	418
Cfr13I	GGNCC	4 cut(s)	190, 280, 584, 745
CseI	GACGC	3 cut(s)	27, 175, 184
Csp6I	GTAC	3 cut(s)	487, 820, 916
CviAII	CATG	8 cut(s)	67, 248, 339, 522, 595, 604, 767, 931
CviQI	GTAC	3 cut(s)	487, 820, 916
DdeI	CTNAG	6 cut(s)	382, 510, 741, 800, 859, 996
DpnI	GATC	2 cut(s)	7, 299
DpnII	GATC	2 cut(s)	5, 297
DriI	GACNNNNNGTC	1 cut(s)	271
EaeI	YGGCCR	1 cut(s)	984
EagI	CGGCCG	1 cut(s)	984
Eam1104I	CTCTTC	1 cut(s)	399
Eam1105I	GACNNNNNGTC	1 cut(s)	271
EarI	CTCTTC	1 cut(s)	399
EciI	GGCGGA	3 cut(s)	176, 203, 212
EclXI	CGGCCG	1 cut(s)	984
Eco130I	CCWWGG	1 cut(s)	276
Eco47I	GGWCC	3 cut(s)	190, 280, 584
Eco52I	CGGCCG	1 cut(s)	984
Eco57I	CTGAAG	1 cut(s)	353
Eco88I	CYCGRG	2 cut(s)	77, 284
EcoO109I	RGGNCCY	1 cut(s)	280
EcoRII	CCWGG	2 cut(s)	56, 192
EcoT14I	CCWWGG	1 cut(s)	276
ErhI	CCWWGG	1 cut(s)	276
Esp3I	CGTCTC	1 cut(s)	158
FaeI	CATG	8 cut(s)	70, 251, 342, 525, 598, 607, 770, 934
FalI	AAGNNNNNCTT	2 cut(s)	905, 937
FaqI	GGGAC	1 cut(s)	908
FatI	CATG	8 cut(s)	66, 247, 338, 521, 594, 603, 766, 930
FbaI	TGATCA	1 cut(s)	297
FblI	GTMKAC	1 cut(s)	1023
Fnu4HI	GCNGC	4 cut(s)	232, 519, 1002, 1038
FokI	GGATG	5 cut(s)	314, 364, 496, 586, 717
Fsp4HI	GCNGC	4 cut(s)	232, 519, 1002, 1038
FspBI	CTAG	1 cut(s)	506
GlaI	GCGC	3 cut(s)	37, 83, 100
GluI	GCNGC	4 cut(s)	232, 519, 1002, 1038
GsuI	CTGGAG	1 cut(s)	460
HaeIII	GGCC	2 cut(s)	747, 986
HapII	CCGG	5 cut(s)	42, 237, 419, 958, 983
HgaI	GACGC	3 cut(s)	27, 175, 184
HhaI	GCGC	3 cut(s)	38, 84, 101
Hin1II	CATG	8 cut(s)	70, 251, 342, 525, 598, 607, 770, 934
Hin6I	GCGC	3 cut(s)	36, 82, 99
HinP1I	GCGC	3 cut(s)	36, 82, 99
HincII	GTYRAC	1 cut(s)	1024
HindII	GTYRAC	1 cut(s)	1024
HindIII	AAGCTT	1 cut(s)	208
HinfI	GANTC	8 cut(s)	17, 72, 121, 272, 431, 502, 973, 979
HpaII	CCGG	5 cut(s)	42, 237, 419, 958, 983
HphI	GGTGA	3 cut(s)	145, 550, 624
Hpy166II	GTNNAC	3 cut(s)	424, 688, 1024
Hpy188I	TCNGA	5 cut(s)	16, 126, 735, 803, 978
Hpy188III	TCNNGA	4 cut(s)	67, 331, 469, 782
Hpy8I	GTNNAC	3 cut(s)	424, 688, 1024
Hpy99I	CGWCG	4 cut(s)	431, 449, 1022, 1025
HpyAV	CCTTC	5 cut(s)	124, 151, 178, 328, 357
HpyCH4III	ACNGT	4 cut(s)	94, 106, 358, 394
HpyCH4IV	ACGT	3 cut(s)	447, 953, 1017
HpyCH4V	TGCA	4 cut(s)	234, 521, 705, 773
HpyF10VI	GCNNNNNNNGC	5 cut(s)	175, 194, 415, 515, 604
HpyF3I	CTNAG	6 cut(s)	382, 510, 741, 800, 859, 996
HpySE526I	ACGT	3 cut(s)	447, 953, 1017
Hsp92II	CATG	8 cut(s)	70, 251, 342, 525, 598, 607, 770, 934
HspAI	GCGC	3 cut(s)	36, 82, 99
Ksp22I	TGATCA	1 cut(s)	297
Kzo9I	GATC	2 cut(s)	5, 297
LguI	GCTCTTC	1 cut(s)	399
LmnI	GCTCC	2 cut(s)	59, 441
Lsp1109I	GCAGC	3 cut(s)	218, 505, 988
LweI	GCATC	4 cut(s)	148, 672, 739, 760
MaeI	CTAG	1 cut(s)	506
MaeII	ACGT	3 cut(s)	447, 953, 1017
MaeIII	GTNAC	5 cut(s)	28, 86, 110, 749, 762
MalI	GATC	2 cut(s)	7, 299
MboI	GATC	2 cut(s)	5, 297
MboII	GAAGA	4 cut(s)	139, 364, 416, 870
MhlI	GDGCHC	1 cut(s)	411
MluCI	AATT	4 cut(s)	452, 706, 777, 1032
MlyI	GAGTC	3 cut(s)	66, 281, 496
MmeI	TCCRAC	3 cut(s)	126, 459, 461
MseI	TTAA	2 cut(s)	351, 1042
MslI	CAYNNNNRTG	1 cut(s)	935
MspI	CCGG	5 cut(s)	42, 237, 419, 958, 983
MspR9I	CCNGG	3 cut(s)	58, 194, 959
MvaI	CCWGG	2 cut(s)	58, 194
MvnI	CGCG	3 cut(s)	38, 84, 99
MwoI	GCNNNNNNNGC	5 cut(s)	175, 194, 415, 515, 604
NciI	CCSGG	1 cut(s)	959
NdeII	GATC	2 cut(s)	5, 297
NlaIII	CATG	8 cut(s)	70, 251, 342, 525, 598, 607, 770, 934
NlaIV	GGNNCC	3 cut(s)	55, 282, 391
NmeAIII	GCCGAG	1 cut(s)	1012
NmuCI	GTSAC	3 cut(s)	28, 110, 762
NspI	RCATGY	2 cut(s)	342, 934
NspV	TTCGAA	1 cut(s)	361
PaeI	GCATGC	1 cut(s)	342
PaeR7I	CTCGAG	1 cut(s)	77
PagI	TCATGA	1 cut(s)	66
PciI	ACATGT	1 cut(s)	930
PciSI	GCTCTTC	1 cut(s)	399
PfeI	GAWTC	5 cut(s)	17, 121, 431, 973, 979
PkrI	GCNGC	4 cut(s)	233, 520, 1003, 1039
PleI	GAGTC	3 cut(s)	66, 280, 496
PpsI	GAGTC	3 cut(s)	66, 280, 496
Ppu21I	YACGTR	1 cut(s)	954
PpuMI	RGGWCCY	1 cut(s)	280
PscI	ACATGT	1 cut(s)	930
Psp5II	RGGWCCY	1 cut(s)	280
Psp6I	CCWGG	2 cut(s)	56, 192
PspGI	CCWGG	2 cut(s)	56, 192
PspN4I	GGNNCC	3 cut(s)	55, 282, 391
PspPI	GGNCC	4 cut(s)	190, 280, 584, 745
PspPPI	RGGWCCY	1 cut(s)	280
PspXI	VCTCGAGB	1 cut(s)	77
RsaI	GTAC	3 cut(s)	488, 821, 917
RsaNI	GTAC	3 cut(s)	487, 820, 916
RseI	CAYNNNNRTG	1 cut(s)	935
SalI	GTCGAC	1 cut(s)	1022
SapI	GCTCTTC	1 cut(s)	399
SaqAI	TTAA	2 cut(s)	351, 1042
SatI	GCNGC	4 cut(s)	232, 519, 1002, 1038
Sau3AI	GATC	2 cut(s)	5, 297
Sau96I	GGNCC	4 cut(s)	190, 280, 584, 745
SchI	GAGTC	3 cut(s)	66, 281, 496
ScrFI	CCNGG	3 cut(s)	58, 194, 959
SduI	GDGCHC	1 cut(s)	411
SfaNI	GCATC	4 cut(s)	148, 672, 739, 760
Sfr274I	CTCGAG	1 cut(s)	77
SfuI	TTCGAA	1 cut(s)	361
SinI	GGWCC	3 cut(s)	190, 280, 584
SlaI	CTCGAG	1 cut(s)	77
SmiMI	CAYNNNNRTG	1 cut(s)	935
SmlI	CTYRAG	1 cut(s)	77
SmoI	CTYRAG	1 cut(s)	77
SphI	GCATGC	1 cut(s)	342
Sse9I	AATT	4 cut(s)	452, 706, 777, 1032
SsiI	CCGC	6 cut(s)	84, 97, 161, 188, 197, 1038
SspI	AATATT	1 cut(s)	826
SspMI	CTAG	1 cut(s)	506
StyD4I	CCNGG	3 cut(s)	56, 192, 957
StyI	CCWWGG	1 cut(s)	276
TaaI	ACNGT	4 cut(s)	94, 106, 358, 394
TaiI	ACGT	3 cut(s)	450, 956, 1020
TaqI	TCGA	6 cut(s)	8, 78, 361, 840, 924, 1023
TasI	AATT	4 cut(s)	452, 706, 777, 1032
TatI	WGTACW	2 cut(s)	486, 915
TauI	GCSGC	1 cut(s)	1040
TfiI	GAWTC	5 cut(s)	17, 121, 431, 973, 979
Tru1I	TTAA	2 cut(s)	351, 1042
Tru9I	TTAA	2 cut(s)	351, 1042
TscAI	CASTG	2 cut(s)	37, 628
TseFI	GTSAC	3 cut(s)	28, 110, 762
TseI	GCWGC	3 cut(s)	231, 518, 1001
Tsp45I	GTSAC	3 cut(s)	28, 110, 762
TspDTI	ATGAA	5 cut(s)	37, 231, 306, 544, 860
TspGWI	ACGGA	2 cut(s)	10, 454
TspRI	CASTG	2 cut(s)	37, 628
VpaK11BI	GGWCC	3 cut(s)	190, 280, 584
XapI	RAATTY	1 cut(s)	452
XceI	RCATGY	2 cut(s)	342, 934
XhoI	CTCGAG	1 cut(s)	77
XmiI	GTMKAC	1 cut(s)	1023
XspI	CTAG	1 cut(s)	506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.