MD15G1024100.v1.1

Bifunctional dihydroflavonol 4-reductase flavanone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002832
Physical Location & Seq
Forward (+)
1433884 .. 1436591
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1024100.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGGATCCGAGTCCGAATCCGTTTGTGTCACCGGGGCGTCCGGTTTCATCGGCTCTTGGCTCGTCATGAGACTCCTAGAGCACGGGTACACCGTCCGCGCCACCGTGCGTGACCCCACGAATCAGAAGAAGGTGAAGCATCTGTTGGACTTGCCGAAGGCGGAGACGCACCTGACGCTGTGGAAGGCGGACTTGGCGGATGAGGGAAGCTTTGATGAAGCCATACAAGGCTGCAGCGGAGTGTTCCATGTTGCTACACCTATGGACTTTGAATCCAAGGACCCCGAGAATGAAGTGATCAAGCCCACAATCAATGGACTGTTGGACATCCTGAAGGCATGCCAGAAAGCAAAAACAGTTCGAAAGCTTGTGTTCACATCCTCAGCAGGAACCGTGAATGTGGAGGAGCATCAAAAGCCGGTCTACGACGAGTCCAACTGGAGTGACGTTGAATTTTGCCGCTCCGTCAAGATGACTGGTTGGATGTACTTCGTCTCCAAGACTCTAGCTGAGCAAGCTGCATGGAAATATGCCAAAGAAAACAACATTGATTTCATCACCATTATCCCAACTCTTGTGATTGGGCCATTTCTCATGCCATCCATGCCACCAAGCCTCATCACTGGACTTTCGCCGATCTTAAGAAATGAATCACATTATGGCATCATCAAGCAGGGCCAGTATGTTCACTTGGACGACCTCTGCCTTTCTCACATTTATCTTTACGAGCATCCGAAAGCCGAAGGCCGTTACATTTGTTCGTCACATGATGCTACAATTCACGAACTTGTAAAAATGCTCAGAGAAAAATACCCCGAATACAATATACCCACAAAGTTCAAGGGCATCGACGACAACTTAGAACCAGTTCATTTTTCTTCAAAGAAGTTGAGGGAGATAGGGTTTGAGTTCAAGTACAGCTTGGAGGACATGTTTGTGGGGGCTGTCGATGCGTGCCGGGCAAAGGGCTTGATTCCGATTCCGATTCCGGCAGAGAAAACTGAGGCTGCTGAGGAGAGCAACCTCGTTGATGTCAAAGTTGGTTAA

Protein Analysis

349

Amino Acids

39.03

Weight (kDa)

5.68

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 143 1.2e-12 NmrA-like family
Epimerase PF01370 8 - 248 5.2e-28 NAD dependent epimerase/dehydratase family
KR PF08659 8 - 138 5.9e-06 KR domain
3Beta_HSD PF01073 10 - 176 1.9e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 267 7.7e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 203 1.2e-13 Male sterility protein
NAD_binding_10 PF13460 12 - 138 8e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 462
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 99
AciI CCGC 6 cut(s) 97, 161, 188, 197, 237, 460
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 353
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 3 cut(s) 89, 488, 917
AfiI CCNNNNNNNGG 1 cut(s) 964
AflII CTTAAG 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 930
AgsI TTSAA 5 cut(s) 272, 452, 841, 882, 913
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 176, 208
AluBI AGCT 5 cut(s) 210, 367, 509, 518, 921
AluI AGCT 5 cut(s) 210, 367, 509, 518, 921
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 3 cut(s) 64, 158, 499
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 3 cut(s) 584, 676, 745
ApeKI GCWGC 4 cut(s) 231, 234, 518, 1007
ApoI RAATTY 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 3 cut(s) 280, 584, 676
AsuC2I CCSGG 2 cut(s) 34, 959
AsuHPI GGTGA 3 cut(s) 22, 145, 550
AsuII TTCGAA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 280
BamHI GGATCC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 84
BbvCI CCTCAGC 2 cut(s) 382, 1011
BbvI GCAGC 4 cut(s) 218, 246, 505, 994
BccI CCATC 1 cut(s) 607
BceAI ACGGC 1 cut(s) 732
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 2 cut(s) 34, 959
BcoDI GTCTC 3 cut(s) 64, 158, 499
BfaI CTAG 2 cut(s) 77, 506
BfmI CTRYAG 1 cut(s) 232
BfrI CTTAAG 1 cut(s) 640
BisI GCNGC 5 cut(s) 232, 235, 460, 519, 1008
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 5 cut(s) 233, 236, 461, 520, 1009
Bme1390I CCNGG 2 cut(s) 34, 959
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 3 cut(s) 280, 584, 676
BmiI GGNNCC 3 cut(s) 7, 282, 391
BmrFI CCNGG 2 cut(s) 34, 959
BmsI GCATC 7 cut(s) 148, 418, 672, 739, 760, 855, 940
BpmI CTGGAG 1 cut(s) 460
Bpu10I CCTNAGC 2 cut(s) 382, 1011
Bpu1102I GCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 361
BpuMI CCSGG 2 cut(s) 34, 959
BsaHI GRCGYC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 33, 276
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 6 cut(s) 479, 509, 887, 917, 917, 947
Bsc4I CCNNNNNNNGG 1 cut(s) 964
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 5 cut(s) 443, 481, 628, 679, 866
BseDI CCNNGG 2 cut(s) 33, 276
BseGI GGATG 6 cut(s) 205, 327, 377, 489, 599, 730
BseLI CCNNNNNNNGG 1 cut(s) 964
BseMII CTCAG 5 cut(s) 396, 501, 814, 993, 1002
BseNI ACTGG 5 cut(s) 443, 481, 628, 679, 866
BseRI GAGGAG 2 cut(s) 419, 1028
BseXI GCAGC 4 cut(s) 218, 246, 505, 994
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 586, 678, 747
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 5 cut(s) 33, 42, 419, 958, 989
BslI CCNNNNNNNGG 1 cut(s) 964
BsmAI GTCTC 3 cut(s) 64, 158, 499
BsmBI CGTCTC 2 cut(s) 158, 499
BsnI GGCC 3 cut(s) 586, 678, 747
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 5, 297, 636
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 6 cut(s) 97, 161, 188, 197, 237, 460
BspANI GGCC 3 cut(s) 586, 678, 747
BspCNI CTCAG 5 cut(s) 395, 502, 813, 994, 1003
BspFNI CGCG 1 cut(s) 99
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 7, 282, 391
BspMAI CTGCAG 1 cut(s) 236
BspPI GGATC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 361
BspTI CTTAAG 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 5 cut(s) 443, 481, 628, 679, 866
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 33, 276
BssMI GATC 3 cut(s) 5, 297, 636
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 276
Bst4CI ACNGT 5 cut(s) 94, 106, 321, 358, 394
BstACI GRCGYC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 640
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 340, 516, 955
BstDEI CTNAG 6 cut(s) 382, 510, 800, 859, 1002, 1011
BstF5I GGATG 6 cut(s) 205, 327, 377, 489, 599, 730
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 3 cut(s) 8, 300, 639
BstMAI GTCTC 3 cut(s) 64, 158, 499
BstMBI GATC 3 cut(s) 5, 297, 636
BstMWI GCNNNNNNNGC 7 cut(s) 175, 194, 415, 515, 604, 950, 959
BstNSI RCATGY 2 cut(s) 342, 934
BstSCI CCNGG 2 cut(s) 32, 957
BstSFI CTRYAG 1 cut(s) 232
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 4 cut(s) 218, 246, 505, 994
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuRI GGCC 3 cut(s) 586, 678, 747
BtsCI GGATG 6 cut(s) 205, 327, 377, 489, 599, 730
BtsIMutI CAGTG 1 cut(s) 621
Cac8I GCNNGC 3 cut(s) 340, 516, 955
CciI TCATGA 1 cut(s) 66
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 3 cut(s) 280, 584, 676
CseI GACGC 3 cut(s) 27, 175, 184
Csp6I GTAC 3 cut(s) 88, 487, 916
CviAII CATG 8 cut(s) 67, 248, 339, 522, 595, 604, 767, 931
CviQI GTAC 3 cut(s) 88, 487, 916
DdeI CTNAG 6 cut(s) 382, 510, 800, 859, 1002, 1011
DpnI GATC 3 cut(s) 7, 299, 638
DpnII GATC 3 cut(s) 5, 297, 636
EciI GGCGGA 3 cut(s) 176, 203, 212
Eco130I CCWWGG 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 2 cut(s) 158, 499
FaeI CATG 8 cut(s) 70, 251, 342, 525, 598, 607, 770, 934
FalI AAGNNNNNCTT 4 cut(s) 176, 208, 905, 937
FatI CATG 8 cut(s) 66, 247, 338, 521, 594, 603, 766, 930
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 232, 235, 460, 519, 1008
FokI GGATG 6 cut(s) 212, 314, 364, 496, 586, 717
Fsp4HI GCNGC 5 cut(s) 232, 235, 460, 519, 1008
FspBI CTAG 2 cut(s) 77, 506
GlaI GCGC 1 cut(s) 100
GluI GCNGC 5 cut(s) 232, 235, 460, 519, 1008
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 3 cut(s) 586, 678, 747
HapII CCGG 5 cut(s) 33, 42, 419, 958, 989
HgaI GACGC 3 cut(s) 27, 175, 184
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 8 cut(s) 70, 251, 342, 525, 598, 607, 770, 934
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HindIII AAGCTT 2 cut(s) 208, 365
HpaII CCGG 5 cut(s) 33, 42, 419, 958, 989
HphI GGTGA 3 cut(s) 22, 145, 550
Hpy166II GTNNAC 4 cut(s) 90, 375, 424, 688
Hpy188I TCNGA 7 cut(s) 10, 16, 126, 735, 803, 978, 984
Hpy188III TCNNGA 4 cut(s) 67, 331, 469, 782
Hpy8I GTNNAC 4 cut(s) 90, 375, 424, 688
Hpy99I CGWCG 2 cut(s) 431, 854
HpyAV CCTTC 5 cut(s) 124, 151, 178, 328, 737
HpyCH4III ACNGT 5 cut(s) 94, 106, 321, 358, 394
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 2 cut(s) 234, 521
HpyF10VI GCNNNNNNNGC 7 cut(s) 175, 194, 415, 515, 604, 950, 959
HpyF3I CTNAG 6 cut(s) 382, 510, 800, 859, 1002, 1011
HpySE526I ACGT 1 cut(s) 447
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 8 cut(s) 70, 251, 342, 525, 598, 607, 770, 934
HspAI GCGC 1 cut(s) 99
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 3 cut(s) 5, 297, 636
LmnI GCTCC 2 cut(s) 406, 467
Lsp1109I GCAGC 4 cut(s) 218, 246, 505, 994
LweI GCATC 7 cut(s) 148, 418, 672, 739, 760, 855, 940
MaeI CTAG 2 cut(s) 77, 506
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 5 cut(s) 28, 110, 443, 749, 762
MalI GATC 3 cut(s) 7, 299, 638
MbiI CCGCTC 1 cut(s) 462
MboI GATC 3 cut(s) 5, 297, 636
MboII GAAGA 2 cut(s) 139, 870
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 2 cut(s) 452, 777
MlyI GAGTC 4 cut(s) 20, 66, 440, 496
MmeI TCCRAC 4 cut(s) 126, 303, 459, 461
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 2 cut(s) 641, 1045
MslI CAYNNNNRTG 1 cut(s) 935
MspA1I CMGCKG 1 cut(s) 237
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 5 cut(s) 33, 42, 419, 958, 989
MspR9I CCNGG 2 cut(s) 34, 959
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 7 cut(s) 175, 194, 415, 515, 604, 950, 959
NciI CCSGG 2 cut(s) 34, 959
NdeII GATC 3 cut(s) 5, 297, 636
NlaIII CATG 8 cut(s) 70, 251, 342, 525, 598, 607, 770, 934
NlaIV GGNNCC 3 cut(s) 7, 282, 391
NmuCI GTSAC 4 cut(s) 28, 110, 443, 762
NspI RCATGY 2 cut(s) 342, 934
NspV TTCGAA 1 cut(s) 361
PaeI GCATGC 1 cut(s) 342
PagI TCATGA 1 cut(s) 66
PciI ACATGT 1 cut(s) 930
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 7 cut(s) 17, 121, 272, 650, 973, 979, 985
PkrI GCNGC 5 cut(s) 233, 236, 461, 520, 1009
PleI GAGTC 4 cut(s) 19, 66, 439, 496
PpsI GAGTC 4 cut(s) 19, 66, 439, 496
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 930
Psp5II RGGWCCY 1 cut(s) 280
PspN4I GGNNCC 3 cut(s) 7, 282, 391
PspPI GGNCC 3 cut(s) 280, 584, 676
PspPPI RGGWCCY 1 cut(s) 280
PstI CTGCAG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 5
RsaI GTAC 3 cut(s) 89, 488, 917
RsaNI GTAC 3 cut(s) 88, 487, 916
RseI CAYNNNNRTG 1 cut(s) 935
SaqAI TTAA 2 cut(s) 641, 1045
SatI GCNGC 5 cut(s) 232, 235, 460, 519, 1008
Sau3AI GATC 3 cut(s) 5, 297, 636
Sau96I GGNCC 3 cut(s) 280, 584, 676
SchI GAGTC 4 cut(s) 20, 66, 440, 496
ScrFI CCNGG 2 cut(s) 34, 959
SduI GDGCHC 1 cut(s) 84
SfaNI GCATC 7 cut(s) 148, 418, 672, 739, 760, 855, 940
SfcI CTRYAG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 361
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SphI GCATGC 1 cut(s) 342
Sse9I AATT 2 cut(s) 452, 777
SsiI CCGC 6 cut(s) 97, 161, 188, 197, 237, 460
SspMI CTAG 2 cut(s) 77, 506
StyD4I CCNGG 2 cut(s) 32, 957
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 5 cut(s) 94, 106, 321, 358, 394
TaiI ACGT 1 cut(s) 450
TaqI TCGA 3 cut(s) 361, 849, 948
TasI AATT 2 cut(s) 452, 777
TatI WGTACW 2 cut(s) 486, 915
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 7 cut(s) 17, 121, 272, 650, 973, 979, 985
Tru1I TTAA 2 cut(s) 641, 1045
Tru9I TTAA 2 cut(s) 641, 1045
TscAI CASTG 1 cut(s) 628
TseFI GTSAC 4 cut(s) 28, 110, 443, 762
TseI GCWGC 4 cut(s) 231, 234, 518, 1007
Tsp45I GTSAC 4 cut(s) 28, 110, 443, 762
TspDTI ATGAA 6 cut(s) 37, 231, 306, 544, 663, 860
TspGWI ACGGA 2 cut(s) 10, 454
TspRI CASTG 1 cut(s) 628
Vha464I CTTAAG 1 cut(s) 640
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 1 cut(s) 452
XceI RCATGY 2 cut(s) 342, 934
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 2 cut(s) 77, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.