Rh6BG424500

Bifunctional dihydroflavonol 4-reductase flavanone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002832
Physical Location & Seq
Reverse (-)
64651916 .. 64655110
3195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG424500.1

Sequence Viewer

Length: 1050 bp
ATGGGATCGGAATCCGAGTCCGTTTGCGTGACAGGCTCCTCCGGTTTCGTAGGTTCATGGCTCGTCATGAGACTCCTAGAGCGCGGCTACACTGTCCGAGCCACCGTGCGAGACCCTGCTAATTCGAAGAAGGTGAAGCATCTGCTGGACTTACCAAAGGCGGCGACTCACTTGACGCTGTGGAAGGCAGACCTGGCGGAGGAGGGAAGCTTCGACGAAGCCATCAAGGGATGCACCGGAGTGTTCCATGTCGCCACTCCTATGGATTTTGAGTCCAAGGACCCTGAGAACGAAGTGATCAAACCTACTATAAATGGGGTGCTAGACATCATGAAAGCATGTCTAAAAGCAAAGACTGTAAGAAGGCTAGTGTTTACAGCTTCGGCTGGATCTGTCAATGTTGAAGAGACCCAAAAGCAGGTCTACAACGAAAGCAACTGGAGTGATGTCGAATTTTGCCGGAGAGTGAAGATGACTGGTTGGATGTATTTTGTATCCAAAACTCTAGCTGAGCAAGAGGCATGGAAGTTTGCCAAAGAAAATAACATTGATTTCATCACCATTATCCCAACTCTCGTAATCGGTCCATTTCTCATGCCTGCCATGCCGCCGAGCCTCATTACTGGACTTTCGCCACTCACTGGAAATGAAAGTCATTATTCGATCATAAAGCAAGGCCAATTCATTCATCTAGACGACCTCTGCCAATCTCATATATACTTGTATGAGCATCCGAAAGCAGAGGGCCGCTACATTTGTTCATCGCACGATGCTACGATTCATGAAATTGCGAAATTGCTGAGGGAAAAGTACCCCGAGTACAATGTTCCTACAACGTTCAAGGGCATTGAGGAGAACTTGCCAAAGGTCCATTTTTCTTCAAAGAAATTATTGGAAACAGGGTTCGAGTTCAAATACAGCTTGGAGGACATGTTTGTAGGAGCTGTTGATGCTTGTAAAGCAAAGGGTTTACTTCCTCCTCCTACTGAAAGAGTTGAAAAGCAGGAGGTTGATGAGAGCAGTGTCGTCCGTGTCAAAGTCACAGGCTAA

Protein Analysis

349

Amino Acids

39.07

Weight (kDa)

6.1

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 129 9.9e-11 NmrA-like family
Epimerase PF01370 8 - 248 1.8e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 173 1.5e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 129 5.4e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 199 6.1e-11 Male sterility protein
NAD_binding_10 PF13460 12 - 144 3.1e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 641
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 84
AciI CCGC 5 cut(s) 84, 161, 197, 608, 748
AclI AACGTT 1 cut(s) 836
AclWI GGATC 2 cut(s) 13, 397
AcsI RAATTY 1 cut(s) 452
AfaI GTAC 2 cut(s) 812, 821
AfiI CCNNNNNNNGG 3 cut(s) 199, 418, 641
AflIII ACRYGT 1 cut(s) 930
AgsI TTSAA 5 cut(s) 404, 841, 882, 913, 998
AjnI CCWGG 1 cut(s) 192
AleI CACNNNNGTG 1 cut(s) 239
AloI GAACNNNNNNTCC 2 cut(s) 887, 919
AluBI AGCT 5 cut(s) 210, 380, 509, 921, 944
AluI AGCT 5 cut(s) 210, 380, 509, 921, 944
Alw26I GTCTC 3 cut(s) 64, 105, 401
AlwI GGATC 2 cut(s) 13, 397
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 2 cut(s) 676, 745
ApoI RAATTY 1 cut(s) 452
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 4 cut(s) 280, 584, 745, 868
AsuHPI GGTGA 2 cut(s) 145, 550
AsuII TTCGAA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 3 cut(s) 280, 584, 868
BbvCI CCTCAGC 1 cut(s) 800
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 505
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 64, 105, 401
BfaI CTAG 5 cut(s) 77, 323, 368, 506, 692
BfuAI ACCTGC 1 cut(s) 409
BfuI GTATCC 1 cut(s) 505
BisI GCNGC 4 cut(s) 85, 162, 608, 748
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 4 cut(s) 86, 163, 609, 749
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 3 cut(s) 280, 584, 868
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 4 cut(s) 280, 584, 745, 868
BmiI GGNNCC 2 cut(s) 37, 282
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 5 cut(s) 148, 221, 739, 760, 940
BpmI CTGGAG 1 cut(s) 460
Bpu10I CCTNAGC 1 cut(s) 800
Bpu1102I GCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 125
BsaBI GATNNNNATC 1 cut(s) 10
BsaI GGTCTC 2 cut(s) 105, 401
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 2 cut(s) 41, 236
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 418, 641
Bse1I ACTGG 4 cut(s) 443, 481, 628, 646
Bse8I GATNNNNATC 1 cut(s) 10
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 3 cut(s) 236, 489, 730
BseJI GATNNNNATC 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 199, 418, 641
BseMII CTCAG 3 cut(s) 276, 501, 791
BseNI ACTGG 4 cut(s) 443, 481, 628, 646
BseRI GAGGAG 4 cut(s) 28, 215, 866, 969
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 678, 747
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 3 cut(s) 42, 237, 460
BslI CCNNNNNNNGG 3 cut(s) 199, 418, 641
BsmAI GTCTC 3 cut(s) 64, 105, 401
BsnI GGCC 2 cut(s) 678, 747
Bso31I GGTCTC 2 cut(s) 105, 401
BsoBI CYCGRG 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 125
Bsp143I GATC 4 cut(s) 5, 297, 389, 663
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 5 cut(s) 84, 161, 197, 608, 748
BspANI GGCC 2 cut(s) 678, 747
BspCNI CTCAG 3 cut(s) 277, 502, 792
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 3 cut(s) 66, 330, 781
BspLI GGNNCC 2 cut(s) 37, 282
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 2 cut(s) 13, 397
BspT104I TTCGAA 1 cut(s) 125
BspTNI GGTCTC 2 cut(s) 105, 401
BsrI ACTGG 4 cut(s) 443, 481, 628, 646
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 4 cut(s) 5, 297, 389, 663
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 94, 106, 358
Bst6I CTCTTC 1 cut(s) 399
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 3 cut(s) 285, 510, 800
BstENI CCTNNNNNAGG 1 cut(s) 197
BstF5I GGATG 3 cut(s) 236, 489, 730
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 4 cut(s) 8, 300, 392, 666
BstMAI GTCTC 3 cut(s) 64, 105, 401
BstMBI GATC 4 cut(s) 5, 297, 389, 663
BstMWI GCNNNNNNNGC 5 cut(s) 33, 194, 604, 950, 959
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 2 cut(s) 342, 934
BstSCI CCNGG 1 cut(s) 192
BstUI CGCG 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 389
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 389
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 2 cut(s) 678, 747
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 236, 489, 730
BtsI GCAGTG 1 cut(s) 1027
BtsIMutI CAGTG 3 cut(s) 90, 639, 1027
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 3 cut(s) 66, 330, 781
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 4 cut(s) 280, 584, 745, 868
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 811, 820
CviQI GTAC 2 cut(s) 811, 820
DdeI CTNAG 3 cut(s) 285, 510, 800
DpnI GATC 4 cut(s) 7, 299, 391, 665
DpnII GATC 4 cut(s) 5, 297, 389, 663
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
EciI GGCGGA 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 276
Eco31I GGTCTC 2 cut(s) 105, 401
Eco47I GGWCC 3 cut(s) 280, 584, 868
Eco88I CYCGRG 1 cut(s) 815
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 4 cut(s) 85, 162, 608, 748
FokI GGATG 3 cut(s) 243, 496, 717
Fsp4HI GCNGC 4 cut(s) 85, 162, 608, 748
FspBI CTAG 5 cut(s) 77, 323, 368, 506, 692
GlaI GCGC 1 cut(s) 83
GluI GCNGC 4 cut(s) 85, 162, 608, 748
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 2 cut(s) 678, 747
HapII CCGG 3 cut(s) 42, 237, 460
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 84
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 6 cut(s) 11, 17, 72, 166, 272, 778
HpaII CCGG 3 cut(s) 42, 237, 460
HphI GGTGA 2 cut(s) 145, 550
Hpy166II GTNNAC 3 cut(s) 375, 424, 971
Hpy188I TCNGA 4 cut(s) 10, 16, 98, 735
Hpy188III TCNNGA 4 cut(s) 67, 331, 692, 782
Hpy8I GTNNAC 3 cut(s) 375, 424, 971
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 124, 178, 357
HpyCH4III ACNGT 3 cut(s) 94, 106, 358
HpyCH4IV ACGT 1 cut(s) 836
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 194, 604, 950, 959
HpyF3I CTNAG 3 cut(s) 285, 510, 800
HpySE526I ACGT 1 cut(s) 836
HspAI GCGC 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 4 cut(s) 5, 297, 389, 663
LmnI GCTCC 2 cut(s) 41, 941
LweI GCATC 5 cut(s) 148, 221, 739, 760, 940
MaeI CTAG 5 cut(s) 77, 323, 368, 506, 692
MaeII ACGT 1 cut(s) 836
MaeIII GTNAC 2 cut(s) 28, 1039
MalI GATC 4 cut(s) 7, 299, 391, 665
MboI GATC 4 cut(s) 5, 297, 389, 663
MboII GAAGA 4 cut(s) 139, 416, 481, 870
MflI RGATCY 1 cut(s) 389
MluCI AATT 6 cut(s) 121, 452, 680, 786, 794, 887
MlyI GAGTC 4 cut(s) 26, 66, 160, 281
MmeI TCCRAC 1 cut(s) 461
MslI CAYNNNNRTG 2 cut(s) 239, 260
MspI CCGG 3 cut(s) 42, 237, 460
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 5 cut(s) 33, 194, 604, 950, 959
NdeII GATC 4 cut(s) 5, 297, 389, 663
NlaIV GGNNCC 2 cut(s) 37, 282
NmeAIII GCCGAG 1 cut(s) 636
NmuCI GTSAC 2 cut(s) 28, 1039
NspI RCATGY 2 cut(s) 342, 934
NspV TTCGAA 1 cut(s) 125
OliI CACNNNNGTG 1 cut(s) 239
PagI TCATGA 3 cut(s) 66, 330, 781
PciI ACATGT 1 cut(s) 930
PfeI GAWTC 2 cut(s) 11, 778
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 4 cut(s) 86, 163, 609, 749
PleI GAGTC 4 cut(s) 25, 66, 160, 280
PpsI GAGTC 4 cut(s) 25, 66, 160, 280
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 930
Psp1406I AACGTT 1 cut(s) 836
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 2 cut(s) 37, 282
PspPI GGNCC 4 cut(s) 280, 584, 745, 868
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 1 cut(s) 389
RsaI GTAC 2 cut(s) 812, 821
RsaNI GTAC 2 cut(s) 811, 820
RseI CAYNNNNRTG 2 cut(s) 239, 260
SatI GCNGC 4 cut(s) 85, 162, 608, 748
Sau3AI GATC 4 cut(s) 5, 297, 389, 663
Sau96I GGNCC 4 cut(s) 280, 584, 745, 868
SchI GAGTC 4 cut(s) 26, 66, 160, 281
ScrFI CCNGG 1 cut(s) 194
SfaNI GCATC 5 cut(s) 148, 221, 739, 760, 940
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 3 cut(s) 280, 584, 868
SmiMI CAYNNNNRTG 2 cut(s) 239, 260
Sse9I AATT 6 cut(s) 121, 452, 680, 786, 794, 887
SsiI CCGC 5 cut(s) 84, 161, 197, 608, 748
SspMI CTAG 5 cut(s) 77, 323, 368, 506, 692
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 94, 106, 358
TaiI ACGT 1 cut(s) 839
TaqI TCGA 5 cut(s) 125, 213, 450, 662, 906
TaqII GACCGA 1 cut(s) 572
TasI AATT 6 cut(s) 121, 452, 680, 786, 794, 887
TatI WGTACW 1 cut(s) 819
TauI GCSGC 4 cut(s) 87, 164, 610, 750
TfiI GAWTC 2 cut(s) 11, 778
TscAI CASTG 3 cut(s) 97, 646, 1027
TseFI GTSAC 2 cut(s) 28, 1039
Tsp45I GTSAC 2 cut(s) 28, 1039
TspDTI ATGAA 9 cut(s) 45, 347, 544, 663, 673, 677, 750, 770, 798
TspGWI ACGGA 2 cut(s) 10, 1019
TspRI CASTG 3 cut(s) 97, 646, 1027
Van91I CCANNNNNTGG 1 cut(s) 641
VpaK11BI GGWCC 3 cut(s) 280, 584, 868
XagI CCTNNNNNAGG 1 cut(s) 197
XapI RAATTY 1 cut(s) 452
XbaI TCTAGA 1 cut(s) 691
XceI RCATGY 2 cut(s) 342, 934
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 5 cut(s) 77, 323, 368, 506, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.