RH6DG417500

Bifunctional dihydroflavonol 4-reductase flavanone

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0002832
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG417500.1

Sequence Viewer

Length: 840 bp
ATGGCATCGGAATCCGAGTCCGTTTGCGTGACAGGCGCCTCCGGTTTCGTCGGTTCATGGCTCGTCATGAGACTCCTAGAGCGCGGCTACACTGTCCGAGCCACCGTGCGAGACCCTGCTAATACGAAGAAGGTCAAGCATCTGCTGGACTTACCAAAGGCGGCGACTCACTTGACGCTGTGGAAGGCAGACCTGGAGGAGGAGGGAAGCTTCGACGAAGTCATCAAGGGATGCACCGGAGTGTTCCATGTCGCCACTCCTATGGATTTTGAGCCCAAGGACCCTGAGAACGAAGTGATCAAACCTGCTATAAATGGGGTGCTAGACATCATGAAAGCATGTCTAAAAGCAAAGACTGTTAGAAGGCTAGTGTTTACATCTTCGGCTGGATCTGTCAGTGCTGAAGAGACCCATAAGCCGGTCTACAACGAAAGCAACTGGAGTGATGTCGAATTTTGCCGGAGAGTGAAGATGACTGGTTGGATGTATTTTGTATCCAAAACTCTAGCTGAGCAAGAGGCATGGAAGTTTGCCAAAGAAAATAACATTGATTTCATCACCATTATCCCAACTCTCGTAATCGGTCCATTTCTCATGCCTGCCATGCCGCCGAGCCTCATTACTGGACTTTCGCCACTCACTGGAAATGAAAGTCATTATTCGATCATAAAGCAAGGCCAATTCATTCATCTAGACGACCTCTGCCAATCTCATATATACTTGTATGAGCATCCGAAAGCAGAGGGCCGCTACATTTGTTCATCGCACGATGCTACGATTCATGAAACTGCGAAATTGCTGAGGGAAAAGTACCCCGAGTACAATGTTCCTACAACGTAA

Protein Analysis

279

Amino Acids

31.28

Weight (kDa)

6.07

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 143 6.6e-12 NmrA-like family
Epimerase PF01370 8 - 248 6.3e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 174 2.6e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 199 4.8e-13 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 129 4.4e-13 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 141 5.6e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 641
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 84
AciI CCGC 4 cut(s) 84, 161, 608, 748
AclWI GGATC 1 cut(s) 397
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 2 cut(s) 812, 821
AfiI CCNNNNNNNGG 3 cut(s) 199, 418, 641
AjnI CCWGG 1 cut(s) 192
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 2 cut(s) 210, 509
AluI AGCT 2 cut(s) 210, 509
Alw26I GTCTC 3 cut(s) 64, 105, 401
AlwI GGATC 1 cut(s) 397
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 2 cut(s) 676, 745
ApoI RAATTY 1 cut(s) 452
AspLEI GCGC 2 cut(s) 38, 84
AspS9I GGNCC 3 cut(s) 280, 584, 745
AsuHPI GGTGA 1 cut(s) 550
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 2 cut(s) 280, 584
BanI GGYRCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 276
BbvCI CCTCAGC 1 cut(s) 800
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 505
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 64, 105, 401
BfaI CTAG 5 cut(s) 77, 323, 368, 506, 692
BfoI RGCGCY 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 313
BfuI GTATCC 1 cut(s) 505
BisI GCNGC 4 cut(s) 85, 162, 608, 748
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 4 cut(s) 86, 163, 609, 749
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 2 cut(s) 280, 584
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 3 cut(s) 280, 584, 745
BmiI GGNNCC 2 cut(s) 37, 282
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 5 cut(s) 14, 148, 221, 739, 760
BpmI CTGGAG 2 cut(s) 215, 460
Bpu10I CCTNAGC 1 cut(s) 800
Bpu1102I GCTNAGC 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 36
BsaI GGTCTC 2 cut(s) 105, 401
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 2 cut(s) 41, 236
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 418, 641
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 4 cut(s) 443, 481, 628, 646
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 3 cut(s) 236, 489, 730
BseLI CCNNNNNNNGG 3 cut(s) 199, 418, 641
BseMII CTCAG 3 cut(s) 276, 501, 791
BseNI ACTGG 4 cut(s) 443, 481, 628, 646
BseRI GAGGAG 2 cut(s) 212, 215
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 678, 747
BshNI GGYRCC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 4 cut(s) 42, 237, 419, 460
BslI CCNNNNNNNGG 3 cut(s) 199, 418, 641
BsmAI GTCTC 3 cut(s) 64, 105, 401
BsnI GGCC 2 cut(s) 678, 747
Bso31I GGTCTC 2 cut(s) 105, 401
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 3 cut(s) 297, 389, 663
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 4 cut(s) 84, 161, 608, 748
BspANI GGCC 2 cut(s) 678, 747
BspCNI CTCAG 3 cut(s) 277, 502, 792
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 3 cut(s) 66, 330, 781
BspLI GGNNCC 2 cut(s) 37, 282
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 1 cut(s) 397
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 2 cut(s) 105, 401
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 4 cut(s) 443, 481, 628, 646
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 297, 389, 663
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 94, 106, 358
Bst6I CTCTTC 1 cut(s) 399
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 3 cut(s) 285, 510, 800
BstENI CCTNNNNNAGG 1 cut(s) 197
BstF5I GGATG 3 cut(s) 236, 489, 730
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 2 cut(s) 38, 84
BstKTI GATC 3 cut(s) 300, 392, 666
BstMAI GTCTC 3 cut(s) 64, 105, 401
BstMBI GATC 3 cut(s) 297, 389, 663
BstMWI GCNNNNNNNGC 2 cut(s) 33, 604
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 192
BstUI CGCG 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 389
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 389
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 2 cut(s) 678, 747
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 236, 489, 730
BtsIMutI CAGTG 3 cut(s) 90, 403, 639
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 3 cut(s) 66, 330, 781
CfoI GCGC 2 cut(s) 38, 84
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 3 cut(s) 280, 584, 745
CseI GACGC 1 cut(s) 184
Csp6I GTAC 2 cut(s) 811, 820
CviAII CATG 9 cut(s) 57, 67, 248, 331, 339, 522, 595, 604, 782
CviQI GTAC 2 cut(s) 811, 820
DdeI CTNAG 3 cut(s) 285, 510, 800
DinI GGCGCC 1 cut(s) 37
DpnI GATC 3 cut(s) 299, 391, 665
DpnII GATC 3 cut(s) 297, 389, 663
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 2 cut(s) 105, 401
Eco47I GGWCC 2 cut(s) 280, 584
Eco57I CTGAAG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 815
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 276
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 9 cut(s) 60, 70, 251, 334, 342, 525, 598, 607, 785
FatI CATG 9 cut(s) 56, 66, 247, 330, 338, 521, 594, 603, 781
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 4 cut(s) 85, 162, 608, 748
FokI GGATG 3 cut(s) 243, 496, 717
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 4 cut(s) 85, 162, 608, 748
FspBI CTAG 5 cut(s) 77, 323, 368, 506, 692
GlaI GCGC 2 cut(s) 37, 83
GluI GCNGC 4 cut(s) 85, 162, 608, 748
GsuI CTGGAG 2 cut(s) 215, 460
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 2 cut(s) 678, 747
HapII CCGG 4 cut(s) 42, 237, 419, 460
HgaI GACGC 1 cut(s) 184
HhaI GCGC 2 cut(s) 38, 84
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 9 cut(s) 60, 70, 251, 334, 342, 525, 598, 607, 785
Hin6I GCGC 2 cut(s) 36, 82
HinP1I GCGC 2 cut(s) 36, 82
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 5 cut(s) 11, 17, 72, 166, 778
HpaII CCGG 4 cut(s) 42, 237, 419, 460
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 375, 424
Hpy188I TCNGA 4 cut(s) 10, 16, 98, 735
Hpy188III TCNNGA 4 cut(s) 67, 331, 692, 782
Hpy8I GTNNAC 2 cut(s) 375, 424
Hpy99I CGWCG 2 cut(s) 53, 218
HpyAV CCTTC 3 cut(s) 124, 178, 357
HpyCH4III ACNGT 3 cut(s) 94, 106, 358
HpyCH4IV ACGT 1 cut(s) 836
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 604
HpyF3I CTNAG 3 cut(s) 285, 510, 800
HpySE526I ACGT 1 cut(s) 836
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 9 cut(s) 60, 70, 251, 334, 342, 525, 598, 607, 785
HspAI GCGC 2 cut(s) 36, 82
KasI GGCGCC 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 3 cut(s) 297, 389, 663
LweI GCATC 5 cut(s) 14, 148, 221, 739, 760
MaeI CTAG 5 cut(s) 77, 323, 368, 506, 692
MaeII ACGT 1 cut(s) 836
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 299, 391, 665
MboI GATC 3 cut(s) 297, 389, 663
MboII GAAGA 4 cut(s) 139, 372, 416, 481
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 3 cut(s) 452, 680, 794
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 3 cut(s) 26, 66, 160
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 9 cut(s) 49, 190, 193, 196, 511, 626, 710, 736, 795
MslI CAYNNNNRTG 2 cut(s) 239, 260
MspI CCGG 4 cut(s) 42, 237, 419, 460
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 33, 604
NarI GGCGCC 1 cut(s) 36
NdeII GATC 3 cut(s) 297, 389, 663
NlaIII CATG 9 cut(s) 60, 70, 251, 334, 342, 525, 598, 607, 785
NlaIV GGNNCC 2 cut(s) 37, 282
NmeAIII GCCGAG 1 cut(s) 636
NmuCI GTSAC 1 cut(s) 28
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 239
PagI TCATGA 3 cut(s) 66, 330, 781
PfeI GAWTC 2 cut(s) 11, 778
PflFI GACNNNGTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 4 cut(s) 86, 163, 609, 749
PleI GAGTC 3 cut(s) 25, 66, 160
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 3 cut(s) 25, 66, 160
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 2 cut(s) 37, 282
PspPI GGNCC 3 cut(s) 280, 584, 745
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 1 cut(s) 389
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 2 cut(s) 812, 821
RsaNI GTAC 2 cut(s) 811, 820
RseI CAYNNNNRTG 2 cut(s) 239, 260
SatI GCNGC 4 cut(s) 85, 162, 608, 748
Sau3AI GATC 3 cut(s) 297, 389, 663
Sau96I GGNCC 3 cut(s) 280, 584, 745
SchI GAGTC 3 cut(s) 26, 66, 160
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 276
SetI ASST 7 cut(s) 135, 195, 212, 307, 511, 702, 839
SfaNI GCATC 5 cut(s) 14, 148, 221, 739, 760
SfoI GGCGCC 1 cut(s) 37
SinI GGWCC 2 cut(s) 280, 584
SmiMI CAYNNNNRTG 2 cut(s) 239, 260
Sse9I AATT 3 cut(s) 452, 680, 794
SsiI CCGC 4 cut(s) 84, 161, 608, 748
SspDI GGCGCC 1 cut(s) 35
SspMI CTAG 5 cut(s) 77, 323, 368, 506, 692
StyD4I CCNGG 1 cut(s) 192
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 94, 106, 358
TaiI ACGT 1 cut(s) 839
TaqI TCGA 3 cut(s) 213, 450, 662
TaqII GACCGA 1 cut(s) 572
TasI AATT 3 cut(s) 452, 680, 794
TatI WGTACW 1 cut(s) 819
TauI GCSGC 4 cut(s) 87, 164, 610, 750
TfiI GAWTC 2 cut(s) 11, 778
TscAI CASTG 3 cut(s) 97, 403, 646
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 9 cut(s) 45, 347, 544, 663, 673, 677, 750, 770, 798
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 3 cut(s) 97, 403, 646
Tth111I GACNNNGTC 1 cut(s) 218
Van91I CCANNNNNTGG 1 cut(s) 641
VpaK11BI GGWCC 2 cut(s) 280, 584
XagI CCTNNNNNAGG 1 cut(s) 197
XapI RAATTY 1 cut(s) 452
XbaI TCTAGA 1 cut(s) 691
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 5 cut(s) 77, 323, 368, 506, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.