MD08G1087500.v1.1

casein kinase II subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002706
Physical Location & Seq
Forward (+)
7248357 .. 7249724
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1087500.v1.1.491

Sequence Viewer

Length: 378 bp
ATGGTGGTATACTTCTCATCTTTAGCCTGGAACCGGAAGATACACTTACATGATCAAATCGTTATGGTTGAAAGATGGTTTTGGGCCTCTGGTTCATATTTAACCCAAAAAGGAAAGGCATCGCTTTGTCTGTCTTTCTGTCAGATGATGCTTAAACGTTGCAGCTGTATCAGCAAAGCTATAGAGCAAAGCAGGAAGCCATGGTCAATATTTATCAATGCGGAAAATCAGCATCTGGTTTCTCCAGAGGCCATTGATTTTCTAGATAAGCTTCTTCGATATGATCACCAGGAAAGGCTTACAGCAAGAGAAGCAATGGCTCATGCATACTTCTCCCAAGTGAGAAGTGCAGAAAATAGCTGGATACGGACGCAGTAA

Protein Analysis

126

Amino Acids

14.74

Weight (kDa)

9.21

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 72 - 111 1.4e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 1 cut(s) 221
AclI AACGTT 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 1 cut(s) 71
AjnI CCWGG 2 cut(s) 26, 288
AluBI AGCT 4 cut(s) 165, 179, 271, 360
AluI AGCT 4 cut(s) 165, 179, 271, 360
AlwNI CAGNNNCTG 1 cut(s) 235
AoxI GGCC 2 cut(s) 84, 249
ApeKI GCWGC 1 cut(s) 162
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 278
BbvI GCAGC 1 cut(s) 174
BccI CCATC 1 cut(s) 69
BciT130I CCWGG 2 cut(s) 28, 290
BciVI GTATCC 1 cut(s) 357
BclI TGATCA 2 cut(s) 52, 283
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 164
Bme1390I CCNGG 2 cut(s) 28, 290
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 32
BmrFI CCNGG 2 cut(s) 28, 290
BmsI GCATC 3 cut(s) 128, 138, 241
BpmI CTGGAG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 2 cut(s) 28, 290
BseDI CCNNGG 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 2 cut(s) 86, 251
BsiSI CCGG 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 33
BsnI GGCC 2 cut(s) 86, 251
Bsp143I GATC 2 cut(s) 52, 283
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 1 cut(s) 221
BspANI GGCC 2 cut(s) 86, 251
BspLI GGNNCC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 321
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 2 cut(s) 52, 283
BssNAI GTATAC 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 200
Bst1107I GTATAC 1 cut(s) 10
Bst2UI CCWGG 2 cut(s) 28, 290
BstDSI CCRYGG 1 cut(s) 200
BstKTI GATC 2 cut(s) 55, 286
BstMBI GATC 2 cut(s) 52, 283
BstMWI GCNNNNNNNGC 2 cut(s) 171, 311
BstNI CCWGG 2 cut(s) 28, 290
BstSCI CCNGG 2 cut(s) 26, 288
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 174
BstZ17I GTATAC 1 cut(s) 10
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 2 cut(s) 86, 251
BtgI CCRYGG 1 cut(s) 200
BtgZI GCGATG 1 cut(s) 105
CaiI CAGNNNCTG 1 cut(s) 235
Cfr13I GGNCC 1 cut(s) 84
CviAII CATG 3 cut(s) 50, 201, 323
DpnI GATC 2 cut(s) 54, 285
DpnII GATC 2 cut(s) 52, 283
Eco130I CCWWGG 1 cut(s) 200
EcoRII CCWGG 2 cut(s) 26, 288
EcoT14I CCWWGG 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 3 cut(s) 53, 204, 326
FaiI YATR 9 cut(s) 10, 51, 65, 97, 182, 202, 282, 324, 328
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FatI CATG 3 cut(s) 49, 200, 322
FbaI TGATCA 2 cut(s) 52, 283
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 1 cut(s) 163
Fsp4HI GCNGC 1 cut(s) 163
FspBI CTAG 1 cut(s) 263
GluI GCNGC 1 cut(s) 163
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 2 cut(s) 86, 251
HapII CCGG 1 cut(s) 34
Hin1II CATG 3 cut(s) 53, 204, 326
HindIII AAGCTT 1 cut(s) 269
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 2 cut(s) 245, 263
Hpy8I GTNNAC 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 3 cut(s) 162, 326, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 311
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 3 cut(s) 53, 204, 326
Ksp22I TGATCA 2 cut(s) 52, 283
Kzo9I GATC 2 cut(s) 52, 283
Lsp1109I GCAGC 1 cut(s) 174
LweI GCATC 3 cut(s) 128, 138, 241
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 157
MalI GATC 2 cut(s) 54, 285
MboI GATC 2 cut(s) 52, 283
MboII GAAGA 2 cut(s) 49, 266
MnlI CCTC 2 cut(s) 97, 241
Mph1103I ATGCAT 1 cut(s) 328
MseI TTAA 2 cut(s) 101, 153
MslI CAYNNNNRTG 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 2 cut(s) 28, 290
MvaI CCWGG 2 cut(s) 28, 290
MwoI GCNNNNNNNGC 2 cut(s) 171, 311
NcoI CCATGG 1 cut(s) 200
NdeII GATC 2 cut(s) 52, 283
NlaIII CATG 3 cut(s) 53, 204, 326
NlaIV GGNNCC 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 328
PkrI GCNGC 1 cut(s) 164
Psp1406I AACGTT 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 26, 288
PspGI CCWGG 2 cut(s) 26, 288
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 165
RseI CAYNNNNRTG 1 cut(s) 48
SaqAI TTAA 2 cut(s) 101, 153
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 2 cut(s) 52, 283
Sau96I GGNCC 1 cut(s) 84
ScrFI CCNGG 2 cut(s) 28, 290
SetI ASST 5 cut(s) 160, 167, 181, 273, 362
SfaNI GCATC 3 cut(s) 128, 138, 241
SfcI CTRYAG 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 48
SsiI CCGC 1 cut(s) 221
SspI AATATT 1 cut(s) 210
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 26, 288
StyI CCWWGG 1 cut(s) 200
TaiI ACGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 277
Tru1I TTAA 2 cut(s) 101, 153
Tru9I TTAA 2 cut(s) 101, 153
TseI GCWGC 1 cut(s) 162
TspDTI ATGAA 1 cut(s) 84
XbaI TCTAGA 1 cut(s) 262
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.