Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002706

Physical Location & Seq

Genomic Coordinates Forward (+)

27585543 .. 27592251

Gene Structure 6709 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1295000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGGCCATAAGGCCCTCCTTGGAATTCACCGTCTCCCACCACCACAACCTTCTCTGCCTCTTCCTTGCGCGCCCCCACCCTCCGTCGTCTCCTCTCTCCTTCTCCTCCCACTTCTTCCACCAGCTCCCGCCGCCTTTCGTCCGCTGCATAGCGTCCTCAGCGACTCCGCTCCCTCACCGCCATACGTCCTTTCACCGCCCCCCGAGGCCACTTCATCCTTATCACGAAACCCTGGCGCAGAAGATCTGTAAAGCGATTCGCCGCCCTGGCGCCCCCTCCAAGGCTAGGACTTACGCCGATGTCAACGTGATCCGACCCAAGGATTACTGGGACTACGAGTCCCTCACCGTCCAATGGGGGGAGCAGGATGATTACGAGGTGGTGAAGAAGGTTGGGAGAGGAAAATACAGTGAGGTGTTTGAGGGGGTTCACTGTACCGATAACGAAAAATGTGTTATCAAGATTCTCAAACCTGTTAAAAAGAAGAAGATTAAGAGGGAGATAAAAATATTGCAGAATCTTTGTGGAGGACCAAATATTGTGAAGTTGCTTGATATTGTCAGAGACCAGCAATCAAAGACCCCTAGTCTTATATTTGAATATGTGAATAATACAGATTTTAAGGTCCTTTATCCGACGCTCTCAGACTTTGACATACGATATTACATCTATGAACTTCTCAAGGCTTTAGATTATTGCCACTCACAAGGTATTATGCATCGAGATGTGAAGCCCCATAATGTTATGATTGATCATGAGCAGCGTAAGCTTCGCCTTATAGATTGGGGCCTTGCCGAGTTTTATCATCCTGAGAAGGAATATAATGTTCGGGTTGCTTCAAGGTATTTCAAAGGTCCTGAACTTCTTGTTGATTTACAAGATTATGATTATTCTTTAGACTTGTGGAGTCTCGGTTGTATGTTAGCTGGAATGATATTTCGTAAGGAGCCATTCTTCTATGGGCATGATAACTATGACCAGCTAGTCAAAATAGCAAAGGTACTTGGGACAGATGAATTAAACGCTTATCTACACAAGTACCACATAGAATTAGACCCACACCTTGCAGCTCTAGTTGGGAGGCATAGCAGGAAACCATGGACAAAATTCATTAATGTTGATAATCAGCACTTAGCAGTTCCTGAGGCGCTTGACTTTATAGACAAGTTGTTGCACTATGATCACCAGGAAAGACCAACTGCAAAAGAAGCAATGGCCCATCCTTATTTCTACCCGATTAGAAATGCAGAAAGCAGCCGGACTCGCACCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0008150 GO:0031974 GO:0031981 GO:0042752 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03008 ko04064 ko04137 ko04139 ko04310 ko04520 ko04712 ko05162 ko05168 ko05169 map03008 map04064 map04137 map04139 map04310 map04520 map04712 map05162 map05168 map05169

Pfam Domains

Protein Families

Pkinase

Protein Analysis

425

Amino Acids

49.65

Weight (kDa)

8.85

Isoelectric Point (pI)

43.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	125 - 410	2.5e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	127 - 326	7.4e-20	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002706)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23070
fragaria_vesca	FvH4_1g17410
malus_domestica	MD02G1183300.v1.1 MD08G1087500.v1.1 MD15G1295000.v1.1
prunus_persica	Prupe.6G222700_v2.0.a1 Prupe.6G222700_v2.0.a1
pyrus_communis	pycom02g14890 pycom02g14900
rosa_chinensis	RchiOBHm_Chr2g0107601 RchiOBHm_Chr4g0423111
rosa_laevigata	RLG00000015367 RLG00000017594 RLG00000019619 RLG00000033266
rosa_multiflora	Rmu_co8147378.1_g000001 Rmu_sc0000800.1_g000031 Rmu_sc0028958.1_g000001
rosa_roxburghii	Rroxscaffold_2G00122390 Rroxscaffold_2G00136160 Rroxscaffold_6G00412260
rosa_rugosa	Rorug01G0255400 Rorug02G0145700 Rorug02G0145800 Rorug02G0145900
rosa_samantha	Rh2AG195200 Rh2BG207800 Rh2CG200400 Rh2CG269000 Rh2DG202800 Rh4AG035300 Rh4AG243500 Rh4BG128100 Rh4BG248200 Rh4CG032800 Rh5CG247100 Rh6AG034200 Rh7AG164100
rosa_wichuraiana	Rw2G015470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	983
AccB1I	GGYRCC	1 cut(s)	269
AccBSI	CCGCTC	1 cut(s)	169
AccII	CGCG	1 cut(s)	70
AciI	CCGC	7 cut(s)	128, 131, 142, 167, 178, 196, 262
AclWI	GGATC	1 cut(s)	304
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	23, 1106
AcyI	GRCGYC	1 cut(s)	270
AfaI	GTAC	3 cut(s)	436, 1002, 1040
AfiI	CCNNNNNNNGG	5 cut(s)	280, 285, 319, 354, 358
AgsI	TTSAA	3 cut(s)	599, 840, 850
AhdI	GACNNNNNGTC	2 cut(s)	337, 585
AjnI	CCWGG	3 cut(s)	231, 265, 1185
AluBI	AGCT	5 cut(s)	124, 769, 926, 982, 1070
AluI	AGCT	5 cut(s)	124, 769, 926, 982, 1070
Alw26I	GTCTC	4 cut(s)	37, 93, 558, 914
AlwI	GGATC	1 cut(s)	304
AlwNI	CAGNNNCTG	1 cut(s)	1142
Ama87I	CYCGRG	1 cut(s)	202
AoxI	GGCC	5 cut(s)	3, 11, 206, 787, 1215
ApeKI	GCWGC	4 cut(s)	144, 760, 1067, 1254
ApoI	RAATTY	2 cut(s)	23, 1106
AseI	ATTAAT	1 cut(s)	1113
AspLEI	GCGC	5 cut(s)	70, 72, 238, 272, 1150
AspS9I	GGNCC	6 cut(s)	12, 530, 625, 787, 854, 1216
AsuHPI	GGTGA	6 cut(s)	19, 167, 185, 337, 394, 1175
AvaI	CYCGRG	1 cut(s)	202
AvaII	GGWCC	3 cut(s)	530, 625, 854
AxyI	CCTNAGG	1 cut(s)	1143
BaeI	ACNNNNGTAYC	2 cut(s)	1022, 1055
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	269
BbvCI	CCTCAGC	1 cut(s)	157
BbvI	GCAGC	4 cut(s)	131, 772, 1079, 1266
BccI	CCATC	1 cut(s)	1227
BciT130I	CCWGG	3 cut(s)	233, 267, 1187
BclI	TGATCA	2 cut(s)	751, 1180
BcoDI	GTCTC	4 cut(s)	37, 93, 558, 914
BfaI	CTAG	4 cut(s)	285, 585, 983, 1073
BfoI	RGCGCY	2 cut(s)	273, 1151
BglI	GCCNNNNNGGC	1 cut(s)	267
BglII	AGATCT	1 cut(s)	243
BisI	GCNGC	6 cut(s)	131, 145, 262, 761, 1068, 1255
BlsI	GCNGC	6 cut(s)	132, 146, 263, 762, 1069, 1256
Bme1390I	CCNGG	3 cut(s)	233, 267, 1187
Bme18I	GGWCC	3 cut(s)	530, 625, 854
BmeRI	GACNNNNNGTC	2 cut(s)	337, 585
BmeT110I	CYCGRG	1 cut(s)	202
BmgT120I	GGNCC	6 cut(s)	12, 530, 625, 787, 854, 1216
BmiI	GGNNCC	3 cut(s)	271, 788, 948
BmrFI	CCNGG	3 cut(s)	233, 267, 1187
BmrI	ACTGGG	2 cut(s)	337, 1264
BmsI	GCATC	1 cut(s)	727
BmuI	ACTGGG	2 cut(s)	337, 1264
Bpu10I	CCTNAGC	1 cut(s)	157
BpuEI	CTTGAG	1 cut(s)	665
BsaHI	GRCGYC	1 cut(s)	270
BsaI	GGTCTC	1 cut(s)	558
BsaJI	CCNNGG	7 cut(s)	18, 203, 231, 265, 279, 318, 1097
BsaXI	ACNNNNNCTCC	2 cut(s)	898, 928
Bsc4I	CCNNNNNNNGG	5 cut(s)	280, 285, 319, 354, 358
Bse1I	ACTGG	2 cut(s)	332, 1270
Bse21I	CCTNAGG	1 cut(s)	1143
Bse3DI	GCAATG	1 cut(s)	1218
BseBI	CCWGG	3 cut(s)	233, 267, 1187
BseDI	CCNNGG	7 cut(s)	18, 203, 231, 265, 279, 318, 1097
BseGI	GGATG	4 cut(s)	214, 373, 805, 1219
BseLI	CCNNNNNNNGG	5 cut(s)	280, 285, 319, 354, 358
BseMI	GCAATG	1 cut(s)	1218
BseMII	CTCAG	4 cut(s)	171, 657, 801, 1134
BseNI	ACTGG	2 cut(s)	332, 1270
BsePI	GCGCGC	1 cut(s)	68
BseRI	GAGGAG	2 cut(s)	81, 94
BseXI	GCAGC	4 cut(s)	131, 772, 1079, 1266
Bsh1236I	CGCG	1 cut(s)	70
BshFI	GGCC	5 cut(s)	5, 13, 208, 789, 1217
BshNI	GGYRCC	1 cut(s)	269
BsiHKCI	CYCGRG	1 cut(s)	202
BsiSI	CCGG	1 cut(s)	1258
BslFI	GGGAC	3 cut(s)	325, 344, 1021
BslI	CCNNNNNNNGG	5 cut(s)	280, 285, 319, 354, 358
BsmAI	GTCTC	4 cut(s)	37, 93, 558, 914
BsmBI	CGTCTC	2 cut(s)	37, 93
BsmFI	GGGAC	3 cut(s)	325, 344, 1021
BsnI	GGCC	5 cut(s)	5, 13, 208, 789, 1217
Bso31I	GGTCTC	1 cut(s)	558
BsoBI	CYCGRG	1 cut(s)	202
Bsp143I	GATC	4 cut(s)	243, 309, 751, 1180
Bsp19I	CCATGG	1 cut(s)	1097
BspACI	CCGC	7 cut(s)	128, 131, 142, 167, 178, 196, 262
BspANI	GGCC	5 cut(s)	5, 13, 208, 789, 1217
BspCNI	CTCAG	4 cut(s)	170, 656, 802, 1135
BspFNI	CGCG	1 cut(s)	70
BspHI	TCATGA	1 cut(s)	754
BspLI	GGNNCC	3 cut(s)	271, 788, 948
BspPI	GGATC	1 cut(s)	304
BspT107I	GGYRCC	1 cut(s)	269
BspTNI	GGTCTC	1 cut(s)	558
BsrBI	CCGCTC	1 cut(s)	169
BsrDI	GCAATG	1 cut(s)	1218
BsrI	ACTGG	2 cut(s)	332, 1270
BssECI	CCNNGG	7 cut(s)	18, 203, 231, 265, 279, 318, 1097
BssHII	GCGCGC	1 cut(s)	68
BssMI	GATC	4 cut(s)	243, 309, 751, 1180
BssNI	GRCGYC	1 cut(s)	270
BssT1I	CCWWGG	4 cut(s)	18, 279, 318, 1097
Bst2UI	CCWGG	3 cut(s)	233, 267, 1187
Bst4CI	ACNGT	4 cut(s)	31, 349, 410, 434
Bst6I	CTCTTC	1 cut(s)	65
BstACI	GRCGYC	1 cut(s)	270
BstC8I	GCNNGC	1 cut(s)	70
BstDEI	CTNAG	5 cut(s)	157, 643, 810, 1132, 1143
BstDSI	CCRYGG	1 cut(s)	1097
BstF5I	GGATG	4 cut(s)	214, 373, 805, 1219
BstFNI	CGCG	1 cut(s)	70
BstH2I	RGCGCY	2 cut(s)	273, 1151
BstHHI	GCGC	5 cut(s)	70, 72, 238, 272, 1150
BstKTI	GATC	4 cut(s)	246, 312, 754, 1183
BstMAI	GTCTC	4 cut(s)	37, 93, 558, 914
BstMBI	GATC	4 cut(s)	243, 309, 751, 1180
BstMWI	GCNNNNNNNGC	6 cut(s)	130, 158, 267, 766, 1208, 1263
BstNI	CCWGG	3 cut(s)	233, 267, 1187
BstSCI	CCNGG	3 cut(s)	231, 265, 1185
BstUI	CGCG	1 cut(s)	70
BstV1I	GCAGC	4 cut(s)	131, 772, 1079, 1266
BstX2I	RGATCY	1 cut(s)	243
BstYI	RGATCY	1 cut(s)	243
Bsu36I	CCTNAGG	1 cut(s)	1143
BsuRI	GGCC	5 cut(s)	5, 13, 208, 789, 1217
BtgI	CCRYGG	1 cut(s)	1097
BtsCI	GGATG	4 cut(s)	214, 373, 805, 1219
BtsIMutI	CAGTG	2 cut(s)	415, 430
Cac8I	GCNNGC	1 cut(s)	70
CaiI	CAGNNNCTG	1 cut(s)	1142
CciI	TCATGA	1 cut(s)	754
CfoI	GCGC	5 cut(s)	70, 72, 238, 272, 1150
Cfr13I	GGNCC	6 cut(s)	12, 530, 625, 787, 854, 1216
CseI	GACGC	2 cut(s)	141, 646
Csp6I	GTAC	3 cut(s)	435, 1001, 1039
CviAII	CATG	3 cut(s)	755, 965, 1098
CviQI	GTAC	3 cut(s)	435, 1001, 1039
DdeI	CTNAG	5 cut(s)	157, 643, 810, 1132, 1143
DinI	GGCGCC	1 cut(s)	271
DpnI	GATC	4 cut(s)	245, 311, 753, 1182
DpnII	GATC	4 cut(s)	243, 309, 751, 1180
DrdI	GACNNNNNNGTC	1 cut(s)	983
DriI	GACNNNNNGTC	2 cut(s)	337, 585
DseDI	GACNNNNNNGTC	1 cut(s)	983
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	65
Eam1105I	GACNNNNNGTC	2 cut(s)	337, 585
EarI	CTCTTC	1 cut(s)	65
Eco130I	CCWWGG	4 cut(s)	18, 279, 318, 1097
Eco31I	GGTCTC	1 cut(s)	558
Eco47I	GGWCC	3 cut(s)	530, 625, 854
Eco81I	CCTNAGG	1 cut(s)	1143
Eco88I	CYCGRG	1 cut(s)	202
EcoO109I	RGGNCCY	4 cut(s)	12, 625, 787, 854
EcoRI	GAATTC	1 cut(s)	23
EcoRII	CCWGG	3 cut(s)	231, 265, 1185
EcoT14I	CCWWGG	4 cut(s)	18, 279, 318, 1097
EcoT22I	ATGCAT	1 cut(s)	720
EgeI	GGCGCC	1 cut(s)	271
EheI	GGCGCC	1 cut(s)	271
ErhI	CCWWGG	4 cut(s)	18, 279, 318, 1097
Esp3I	CGTCTC	2 cut(s)	37, 93
FaeI	CATG	3 cut(s)	758, 968, 1101
FaqI	GGGAC	3 cut(s)	325, 344, 1021
FatI	CATG	3 cut(s)	754, 964, 1097
FauI	CCCGC	1 cut(s)	135
FbaI	TGATCA	2 cut(s)	751, 1180
Fnu4HI	GCNGC	6 cut(s)	131, 145, 262, 761, 1068, 1255
FokI	GGATG	4 cut(s)	201, 380, 792, 1206
Fsp4HI	GCNGC	6 cut(s)	131, 145, 262, 761, 1068, 1255
FspBI	CTAG	4 cut(s)	285, 585, 983, 1073
GlaI	GCGC	5 cut(s)	69, 71, 237, 271, 1149
GluI	GCNGC	6 cut(s)	131, 145, 262, 761, 1068, 1255
HaeII	RGCGCY	2 cut(s)	273, 1151
HaeIII	GGCC	5 cut(s)	5, 13, 208, 789, 1217
HapII	CCGG	1 cut(s)	1258
HgaI	GACGC	2 cut(s)	141, 646
HhaI	GCGC	5 cut(s)	70, 72, 238, 272, 1150
Hin1I	GRCGYC	1 cut(s)	270
Hin1II	CATG	3 cut(s)	758, 968, 1101
Hin6I	GCGC	5 cut(s)	68, 70, 236, 270, 1148
HinP1I	GCGC	5 cut(s)	68, 70, 236, 270, 1148
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HindIII	AAGCTT	1 cut(s)	767
HinfI	GANTC	7 cut(s)	163, 256, 338, 463, 517, 907, 1261
HpaII	CCGG	1 cut(s)	1258
HphI	GGTGA	6 cut(s)	19, 167, 185, 337, 394, 1175
Hpy166II	GTNNAC	2 cut(s)	304, 430
Hpy188I	TCNGA	4 cut(s)	314, 563, 636, 646
Hpy188III	TCNNGA	7 cut(s)	224, 460, 722, 755, 809, 857, 1142
Hpy8I	GTNNAC	2 cut(s)	304, 430
Hpy99I	CGWCG	2 cut(s)	88, 640
HpyAV	CCTTC	4 cut(s)	59, 109, 382, 808
HpyCH4III	ACNGT	4 cut(s)	31, 349, 410, 434
HpyCH4IV	ACGT	2 cut(s)	185, 306
HpyCH4V	TGCA	7 cut(s)	147, 514, 718, 1067, 1174, 1202, 1247
HpyF10VI	GCNNNNNNNGC	6 cut(s)	130, 158, 267, 766, 1208, 1263
HpyF3I	CTNAG	5 cut(s)	157, 643, 810, 1132, 1143
HpySE526I	ACGT	2 cut(s)	185, 306
Hsp92I	GRCGYC	1 cut(s)	270
Hsp92II	CATG	3 cut(s)	758, 968, 1101
HspAI	GCGC	5 cut(s)	68, 70, 236, 270, 1148
KasI	GGCGCC	1 cut(s)	269
Ksp22I	TGATCA	2 cut(s)	751, 1180
Kzo9I	GATC	4 cut(s)	243, 309, 751, 1180
LmnI	GCTCC	4 cut(s)	129, 174, 361, 946
Lsp1109I	GCAGC	4 cut(s)	131, 772, 1079, 1266
LweI	GCATC	1 cut(s)	727
MaeI	CTAG	4 cut(s)	285, 585, 983, 1073
MaeII	ACGT	2 cut(s)	185, 306
MalI	GATC	4 cut(s)	245, 311, 753, 1182
MbiI	CCGCTC	1 cut(s)	169
MboI	GATC	4 cut(s)	243, 309, 751, 1180
MboII	GAAGA	7 cut(s)	52, 106, 253, 397, 496, 499, 946
MflI	RGATCY	1 cut(s)	243
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	4 cut(s)	23, 1016, 1049, 1106
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	270
MlyI	GAGTC	4 cut(s)	157, 347, 916, 1255
MmeI	TCCRAC	2 cut(s)	337, 659
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	720
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	5 cut(s)	477, 492, 621, 1019, 1113
MslI	CAYNNNNRTG	1 cut(s)	723
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	144
MspI	CCGG	1 cut(s)	1258
MspR9I	CCNGG	3 cut(s)	233, 267, 1187
MvaI	CCWGG	3 cut(s)	233, 267, 1187
MvnI	CGCG	1 cut(s)	70
MwoI	GCNNNNNNNGC	6 cut(s)	130, 158, 267, 766, 1208, 1263
NarI	GGCGCC	1 cut(s)	270
NcoI	CCATGG	1 cut(s)	1097
NdeII	GATC	4 cut(s)	243, 309, 751, 1180
NlaIII	CATG	3 cut(s)	758, 968, 1101
NlaIV	GGNNCC	3 cut(s)	271, 788, 948
NmeAIII	GCCGAG	1 cut(s)	820
NsiI	ATGCAT	1 cut(s)	720
PagI	TCATGA	1 cut(s)	754
PauI	GCGCGC	1 cut(s)	68
PfeI	GAWTC	3 cut(s)	256, 463, 517
PkrI	GCNGC	6 cut(s)	132, 146, 263, 762, 1069, 1256
PleI	GAGTC	4 cut(s)	157, 346, 915, 1255
PluTI	GGCGCC	1 cut(s)	273
PpsI	GAGTC	4 cut(s)	157, 346, 915, 1255
PpuMI	RGGWCCY	2 cut(s)	625, 854
PshBI	ATTAAT	1 cut(s)	1113
Psp5II	RGGWCCY	2 cut(s)	625, 854
Psp6I	CCWGG	3 cut(s)	231, 265, 1185
PspGI	CCWGG	3 cut(s)	231, 265, 1185
PspN4I	GGNNCC	3 cut(s)	271, 788, 948
PspPI	GGNCC	6 cut(s)	12, 530, 625, 787, 854, 1216
PspPPI	RGGWCCY	2 cut(s)	625, 854
PstNI	CAGNNNCTG	1 cut(s)	1142
PsuI	RGATCY	1 cut(s)	243
PteI	GCGCGC	1 cut(s)	68
RsaI	GTAC	3 cut(s)	436, 1002, 1040
RsaNI	GTAC	3 cut(s)	435, 1001, 1039
RseI	CAYNNNNRTG	1 cut(s)	723
SaqAI	TTAA	5 cut(s)	477, 492, 621, 1019, 1113
SatI	GCNGC	6 cut(s)	131, 145, 262, 761, 1068, 1255
Sau3AI	GATC	4 cut(s)	243, 309, 751, 1180
Sau96I	GGNCC	6 cut(s)	12, 530, 625, 787, 854, 1216
SchI	GAGTC	4 cut(s)	157, 347, 916, 1255
ScrFI	CCNGG	3 cut(s)	233, 267, 1187
SfaNI	GCATC	1 cut(s)	727
SfoI	GGCGCC	1 cut(s)	271
SinI	GGWCC	3 cut(s)	530, 625, 854
SmiMI	CAYNNNNRTG	1 cut(s)	723
SmlI	CTYRAG	1 cut(s)	680
SmoI	CTYRAG	1 cut(s)	680
Sse9I	AATT	4 cut(s)	23, 1016, 1049, 1106
SsiI	CCGC	7 cut(s)	128, 131, 142, 167, 178, 196, 262
SspDI	GGCGCC	1 cut(s)	269
SspI	AATATT	2 cut(s)	510, 538
SspMI	CTAG	4 cut(s)	285, 585, 983, 1073
StyD4I	CCNGG	3 cut(s)	231, 265, 1185
StyI	CCWWGG	4 cut(s)	18, 279, 318, 1097
TaaI	ACNGT	4 cut(s)	31, 349, 410, 434
TaiI	ACGT	2 cut(s)	188, 309
TaqI	TCGA	1 cut(s)	721
TasI	AATT	4 cut(s)	23, 1016, 1049, 1106
TauI	GCSGC	2 cut(s)	133, 264
TfiI	GAWTC	3 cut(s)	256, 463, 517
Tru1I	TTAA	5 cut(s)	477, 492, 621, 1019, 1113
Tru9I	TTAA	5 cut(s)	477, 492, 621, 1019, 1113
TscAI	CASTG	2 cut(s)	415, 437
TseI	GCWGC	4 cut(s)	144, 760, 1067, 1254
TspDTI	ATGAA	4 cut(s)	203, 687, 1029, 1099
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	2 cut(s)	415, 437
VpaK11BI	GGWCC	3 cut(s)	530, 625, 854
VspI	ATTAAT	1 cut(s)	1113
XapI	RAATTY	2 cut(s)	23, 1106
XspI	CTAG	4 cut(s)	285, 585, 983, 1073
Zsp2I	ATGCAT	1 cut(s)	720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.