Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Reverse (-)

9694053 .. 9698017

Gene Structure 3965 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1110200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1791 bp

ATGCTCCTCCGCCTCCCTACTCTCCTCCGCACCCTCCACCCTCCCTCCCCGTCCTCCGCCACTCAAATGGCGCGTTACATTCCGCGCGCCACCCTAATATCCATCACCCCTCTTCCCATCCGACAAACCACCGCCTCATTTCCCGCGCTCATCCATTCAATCTCCCCTCCTGAAACGCACCGTTTCAACACCTCAAAAACTTCTCTATTCGGCCGGAGATTTCACTCTCTGTGCGGACACGAACGGATACGAGCAGCCAGACCGCCATTGCTACGGGGACCAATCTTCTTCTCACAGAGAGAGTACCGAAAGGTGAGACGGCGAGCCGCAAAAAGCAAGGAGAAGGAGATCGAGCTTGACATCAGCATTTGCATCGAAGAAGGATTGCCTGACGATCCTGAAGTGCTGAGTATTGCGGAATTGCTTCGTCTGAATGTTCCAATGGCAATGAAGCTGGCGTTTGATGGGTTGAAAGGTTCAGTGTATAAAACAAGGGACAATTCCATAAACGACGTTGGTGGATTCGAAAGCATAGAGCTTTCGGTGCTTCTCTGCAACGATGAGTTCATCCGAAAACTTAACAAGGAATGGAGAAATGAAGACCATGCCACCGATGTTCTCTCACTGTCGCAGCATGTCCCAAATCTTAAGCTTCCCATTCTTATGTTGGGTGACATTGTGATTTCTGTTGAGACAGCGGCAAGGCAAGCGGAGGAAAGAGGCCACACGCTTATTGATGAGATACGTGTTCTCATGATACATGGGTTGTTACATTTACTAGGATTTGATCATGAGATTAGTGAAGAGGCAGAAGTGGAAATGGAGAAGGAGGAGGAGCTTCTTTTAAAGAGTCTTGGGTGGAAGGGGAAGGGATTAATTCAAAGTGCGCATGATGCCGAAACCCAAACAAATTCCCATGAGGAAAAACCAGATGACAGGAAGAGGGAAGGCAGCCTGCGATTTTATAAACCAAAGTTCAAATGCATTTTCTGTGATATGGATGGCACCCTTCTCAACAGCAAAAGTCAAATTAGTTTAACAACTGCGAAAGCTCTCAAGGAGGTCTCATCAAGGGGTGTGAGAGTAGTGATAGCCACTGGAAAATCTCGTTCAGCTGTGATGAAAATTTTTAAGGAGGTAGAATTAGCTGGAGAAGATGGCATTGTTTCGGAATTTTCTCCTGGGGTTTTTTTGCAGGGGTTGCTTGTTTATGGTAGGCAAGGTCGGGAAATTTTTAGAAAAAATTTAGATACAAGTGTCTGCAGAGAGGCGTGTCTCTACTCTTGTGAGAATAAGGTTCCTCTTATTGCGTTCAGCAAGGACCGTTGCTTTTCTCTGTTTGAGCATCCATTACTTGACTCAGTGCATACATTGTATAATGAGCCAAAGGTGGAAATCATGCCTTCAGTTGAGCACCTCTTGGCTGAAGCTGACAAAGTGCTCTTCATGGAGACCCCTGAGGTAGTGGCTACTACTTTGAGGCCATACTGGTCACAAGCAACTGGAGATCGTGCAACAGTTGTTCAAGCTGTGGAAGGGCACCTTGAAATTGTTCCCGCAGGAACCTCAAAGGGAAGTGGGGTGAACATGCTTCTTGATCATTTAGGAATCACTCGAAATCAGATCATGGCTATTGGCGATGGAGAAAATGATGTCGAGATGCTTGAGCTGGCTTCTTTGGGCGTTGCTCTTAGTAACGGATCAGAGAAGACAAAAGCTGTGGCTAATGCAATTGGTGCAAGCAATGATGAAGATGGTGCGGCTGATGCCATTTACCGGTACGCATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0042578 GO:0043167 GO:0043169 GO:0044237 GO:0046872 GO:0050308

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

597

Amino Acids

66.33

Weight (kDa)

6.03

Isoelectric Point (pI)

36.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	176 - 284	5.4e-33	Endoribonuclease YbeY
Hydrolase_3	PF08282	329 - 591	1.2e-51	haloacid dehalogenase-like hydrolase
S6PP	PF05116	502 - 570	2e-09	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	966
Acc16I	TGCGCA	1 cut(s)	888
AccB1I	GGYRCC	2 cut(s)	1004, 1539
AccII	CGCG	4 cut(s)	73, 85, 87, 146
AclWI	GGATC	2 cut(s)	389, 1708
AcoI	YGGCCR	1 cut(s)	211
AcsI	RAATTY	5 cut(s)	910, 1125, 1172, 1230, 1243
AcuI	CTGAAG	3 cut(s)	420, 1389, 1446
AfaI	GTAC	2 cut(s)	305, 1781
AfiI	CCNNNNNNNGG	1 cut(s)	1776
AflII	CTTAAG	1 cut(s)	647
AflIII	ACRYGT	1 cut(s)	745
AgeI	ACCGGT	1 cut(s)	1776
AgsI	TTSAA	7 cut(s)	159, 187, 472, 881, 979, 1526, 1547
AjnI	CCWGG	1 cut(s)	1180
Alw21I	GWGCWC	2 cut(s)	1416, 1443
Alw26I	GTCTC	5 cut(s)	310, 686, 1069, 1280, 1445
AlwI	GGATC	2 cut(s)	389, 1708
AoxI	GGCC	3 cut(s)	211, 721, 1481
ApeKI	GCWGC	3 cut(s)	254, 631, 951
ApoI	RAATTY	5 cut(s)	910, 1125, 1172, 1230, 1243
AseI	ATTAAT	1 cut(s)	875
AsiGI	ACCGGT	1 cut(s)	1776
Asp700I	GAANNNNTTC	2 cut(s)	423, 1551
AspLEI	GCGC	5 cut(s)	73, 87, 89, 148, 889
AspS9I	GGNCC	2 cut(s)	278, 1321
AsuHPI	GGTGA	4 cut(s)	97, 325, 683, 1594
AsuII	TTCGAA	1 cut(s)	525
AvaII	GGWCC	2 cut(s)	278, 1321
AxyI	CCTNAGG	1 cut(s)	1458
BaeGI	GKGCMC	1 cut(s)	1542
BaeI	ACNNNNGTAYC	4 cut(s)	749, 782, 1242, 1275
BanI	GGYRCC	2 cut(s)	1004, 1539
BbsI	GAAGAC	2 cut(s)	606, 1715
Bbv12I	GWGCWC	2 cut(s)	1416, 1443
BbvI	GCAGC	3 cut(s)	266, 643, 963
BccI	CCATC	7 cut(s)	110, 125, 458, 995, 1151, 1634, 1748
BceAI	ACGGC	1 cut(s)	335
BcgI	CGANNNNNNTGC	2 cut(s)	355, 389
BciT130I	CCWGG	1 cut(s)	1182
BciVI	GTATCC	1 cut(s)	240
BclI	TGATCA	2 cut(s)	787, 1597
BcoDI	GTCTC	5 cut(s)	310, 686, 1069, 1280, 1445
BfaI	CTAG	1 cut(s)	779
BfmI	CTRYAG	1 cut(s)	1261
BfrI	CTTAAG	1 cut(s)	647
BfuI	GTATCC	1 cut(s)	240
BisI	GCNGC	6 cut(s)	255, 327, 632, 699, 952, 1761
BlsI	GCNGC	6 cut(s)	256, 328, 633, 700, 953, 1762
Bme1390I	CCNGG	1 cut(s)	1182
Bme18I	GGWCC	2 cut(s)	278, 1321
BmgT120I	GGNCC	2 cut(s)	278, 1321
BmiI	GGNNCC	5 cut(s)	279, 1006, 1299, 1541, 1564
BmrFI	CCNGG	1 cut(s)	1182
BmsI	GCATC	5 cut(s)	381, 883, 1354, 1650, 1756
BpiI	GAAGAC	2 cut(s)	606, 1715
BpmI	CTGGAG	2 cut(s)	1170, 1524
Bpu14I	TTCGAA	1 cut(s)	525
BpuEI	CTTGAG	2 cut(s)	1040, 1685
BsaAI	YACGTR	1 cut(s)	746
BsaI	GGTCTC	2 cut(s)	1069, 1445
BsaJI	CCNNGG	1 cut(s)	1181
BsaWI	WCCGGW	1 cut(s)	1776
BsaXI	ACNNNNNCTCC	2 cut(s)	29, 59
Bsc4I	CCNNNNNNNGG	1 cut(s)	1776
Bse118I	RCCGGY	1 cut(s)	1776
Bse1I	ACTGG	3 cut(s)	1102, 1493, 1507
Bse21I	CCTNAGG	1 cut(s)	1458
Bse3DI	GCAATG	3 cut(s)	266, 453, 1750
BseBI	CCWGG	1 cut(s)	1182
BseDI	CCNNGG	1 cut(s)	1181
BseGI	GGATG	5 cut(s)	117, 150, 567, 1006, 1345
BseLI	CCNNNNNNNGG	1 cut(s)	1776
BseMI	GCAATG	3 cut(s)	266, 453, 1750
BseMII	CTCAG	3 cut(s)	398, 1374, 1449
BseNI	ACTGG	3 cut(s)	1102, 1493, 1507
BsePI	GCGCGC	1 cut(s)	85
BseRI	GAGGAG	3 cut(s)	14, 845, 848
BseSI	GKGCMC	1 cut(s)	1542
BseX3I	CGGCCG	1 cut(s)	211
BseXI	GCAGC	3 cut(s)	266, 643, 963
Bsh1236I	CGCG	4 cut(s)	73, 85, 87, 146
Bsh1285I	CGRYCG	1 cut(s)	214
BshFI	GGCC	3 cut(s)	213, 723, 1483
BshNI	GGYRCC	2 cut(s)	1004, 1539
BshTI	ACCGGT	1 cut(s)	1776
BsiEI	CGRYCG	1 cut(s)	214
BsiHKAI	GWGCWC	2 cut(s)	1416, 1443
BsiSI	CCGG	2 cut(s)	214, 1777
BslFI	GGGAC	3 cut(s)	291, 509, 623
BslI	CCNNNNNNNGG	1 cut(s)	1776
BsmAI	GTCTC	5 cut(s)	310, 686, 1069, 1280, 1445
BsmBI	CGTCTC	1 cut(s)	310
BsmFI	GGGAC	3 cut(s)	291, 509, 623
BsnI	GGCC	3 cut(s)	213, 723, 1483
Bso31I	GGTCTC	2 cut(s)	1069, 1445
Bsp119I	TTCGAA	1 cut(s)	525
Bsp1286I	GDGCHC	3 cut(s)	1416, 1443, 1542
Bsp143I	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
BspANI	GGCC	3 cut(s)	213, 723, 1483
BspCNI	CTCAG	3 cut(s)	399, 1373, 1450
BspFNI	CGCG	4 cut(s)	73, 85, 87, 146
BspHI	TCATGA	2 cut(s)	753, 790
BspLI	GGNNCC	5 cut(s)	279, 1006, 1299, 1541, 1564
BspMAI	CTGCAG	1 cut(s)	1265
BspPI	GGATC	2 cut(s)	389, 1708
BspQI	GCTCTTC	1 cut(s)	1448
BspT104I	TTCGAA	1 cut(s)	525
BspT107I	GGYRCC	2 cut(s)	1004, 1539
BspTI	CTTAAG	1 cut(s)	647
BspTNI	GGTCTC	2 cut(s)	1069, 1445
BsrDI	GCAATG	3 cut(s)	266, 453, 1750
BsrFI	RCCGGY	1 cut(s)	1776
BsrI	ACTGG	3 cut(s)	1102, 1493, 1507
BssAI	RCCGGY	1 cut(s)	1776
BssECI	CCNNGG	1 cut(s)	1181
BssHII	GCGCGC	1 cut(s)	85
BssMI	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
Bst2UI	CCWGG	1 cut(s)	1182
Bst4CI	ACNGT	4 cut(s)	182, 627, 1325, 1519
Bst6I	CTCTTC	4 cut(s)	117, 798, 935, 1448
BstAFI	CTTAAG	1 cut(s)	647
BstAPI	GCANNNNNTGC	2 cut(s)	1201, 1736
BstBAI	YACGTR	1 cut(s)	746
BstBI	TTCGAA	1 cut(s)	525
BstC8I	GCNNGC	7 cut(s)	87, 324, 456, 708, 956, 1671, 1741
BstDEI	CTNAG	4 cut(s)	407, 1360, 1458, 1691
BstF5I	GGATG	5 cut(s)	117, 150, 567, 1006, 1345
BstFNI	CGCG	4 cut(s)	73, 85, 87, 146
BstHHI	GCGC	5 cut(s)	73, 87, 89, 148, 889
BstKTI	GATC	7 cut(s)	351, 397, 790, 1510, 1600, 1626, 1703
BstMAI	GTCTC	5 cut(s)	310, 686, 1069, 1280, 1445
BstMBI	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
BstMCI	CGRYCG	1 cut(s)	214
BstMWI	GCNNNNNNNGC	6 cut(s)	544, 707, 893, 1201, 1736, 1766
BstNI	CCWGG	1 cut(s)	1182
BstNSI	RCATGY	2 cut(s)	638, 1591
BstSCI	CCNGG	1 cut(s)	1180
BstSFI	CTRYAG	1 cut(s)	1261
BstSLI	GKGCMC	1 cut(s)	1542
BstUI	CGCG	4 cut(s)	73, 85, 87, 146
BstV1I	GCAGC	3 cut(s)	266, 643, 963
BstV2I	GAAGAC	2 cut(s)	606, 1715
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstZI	CGGCCG	1 cut(s)	211
Bsu36I	CCTNAGG	1 cut(s)	1458
BsuI	GTATCC	1 cut(s)	240
BsuRI	GGCC	3 cut(s)	213, 723, 1483
BtgZI	GCGATG	1 cut(s)	1653
BtsCI	GGATG	5 cut(s)	117, 150, 567, 1006, 1345
BtsIMutI	CAGTG	4 cut(s)	486, 623, 1095, 1368
Cac8I	GCNNGC	7 cut(s)	87, 324, 456, 708, 956, 1671, 1741
CciI	TCATGA	2 cut(s)	753, 790
CfoI	GCGC	5 cut(s)	73, 87, 89, 148, 889
Cfr10I	RCCGGY	1 cut(s)	1776
Cfr13I	GGNCC	2 cut(s)	278, 1321
Csp6I	GTAC	2 cut(s)	304, 1780
CspAI	ACCGGT	1 cut(s)	1776
CspCI	CAANNNNNGTGG	2 cut(s)	1701, 1736
CviQI	GTAC	2 cut(s)	304, 1780
DdeI	CTNAG	4 cut(s)	407, 1360, 1458, 1691
DpnI	GATC	7 cut(s)	350, 396, 789, 1509, 1599, 1625, 1702
DpnII	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
DraI	TTTAAA	1 cut(s)	846
EaeI	YGGCCR	1 cut(s)	211
EagI	CGGCCG	1 cut(s)	211
Eam1104I	CTCTTC	4 cut(s)	117, 798, 935, 1448
EarI	CTCTTC	4 cut(s)	117, 798, 935, 1448
EciI	GGCGGA	1 cut(s)	46
EclXI	CGGCCG	1 cut(s)	211
Eco31I	GGTCTC	2 cut(s)	1069, 1445
Eco47I	GGWCC	2 cut(s)	278, 1321
Eco52I	CGGCCG	1 cut(s)	211
Eco57I	CTGAAG	3 cut(s)	420, 1389, 1446
Eco81I	CCTNAGG	1 cut(s)	1458
EcoRII	CCWGG	1 cut(s)	1180
EcoT22I	ATGCAT	1 cut(s)	986
Esp3I	CGTCTC	1 cut(s)	310
FalI	AAGNNNNNCTT	2 cut(s)	1527, 1559
FaqI	GGGAC	3 cut(s)	291, 509, 623
FauI	CCCGC	2 cut(s)	151, 1564
FbaI	TGATCA	2 cut(s)	787, 1597
Fnu4HI	GCNGC	6 cut(s)	255, 327, 632, 699, 952, 1761
FokI	GGATG	5 cut(s)	104, 137, 554, 1013, 1332
Fsp4HI	GCNGC	6 cut(s)	255, 327, 632, 699, 952, 1761
FspAI	RTGCGCAY	1 cut(s)	888
FspBI	CTAG	1 cut(s)	779
FspI	TGCGCA	1 cut(s)	888
GlaI	GCGC	5 cut(s)	72, 86, 88, 147, 888
GluI	GCNGC	6 cut(s)	255, 327, 632, 699, 952, 1761
GsuI	CTGGAG	2 cut(s)	1170, 1524
HaeIII	GGCC	3 cut(s)	213, 723, 1483
HapII	CCGG	2 cut(s)	214, 1777
HhaI	GCGC	5 cut(s)	73, 87, 89, 148, 889
Hin6I	GCGC	5 cut(s)	71, 85, 87, 146, 887
HinP1I	GCGC	5 cut(s)	71, 85, 87, 146, 887
HindIII	AAGCTT	1 cut(s)	650
HinfI	GANTC	4 cut(s)	522, 850, 1358, 1608
HpaII	CCGG	2 cut(s)	214, 1777
HphI	GGTGA	4 cut(s)	97, 325, 683, 1594
Hpy166II	GTNNAC	1 cut(s)	1585
Hpy188I	TCNGA	6 cut(s)	122, 432, 572, 1171, 1623, 1705
Hpy188III	TCNNGA	7 cut(s)	170, 398, 754, 791, 1226, 1595, 1657
Hpy8I	GTNNAC	1 cut(s)	1585
Hpy99I	CGWCG	1 cut(s)	515
HpyAV	CCTTC	9 cut(s)	337, 374, 820, 856, 862, 941, 1019, 1413, 1529
HpyCH4III	ACNGT	4 cut(s)	182, 627, 1325, 1519
HpyCH4IV	ACGT	2 cut(s)	513, 745
HpyCH4V	TGCA	9 cut(s)	372, 555, 984, 1195, 1263, 1366, 1514, 1730, 1739
HpyF10VI	GCNNNNNNNGC	6 cut(s)	544, 707, 893, 1201, 1736, 1766
HpyF3I	CTNAG	4 cut(s)	407, 1360, 1458, 1691
HpySE526I	ACGT	2 cut(s)	513, 745
HspAI	GCGC	5 cut(s)	71, 85, 87, 146, 887
Ksp22I	TGATCA	2 cut(s)	787, 1597
Kzo9I	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
LguI	GCTCTTC	1 cut(s)	1448
LmnI	GCTCC	2 cut(s)	9, 835
Lsp1109I	GCAGC	3 cut(s)	266, 643, 963
LweI	GCATC	5 cut(s)	381, 883, 1354, 1650, 1756
MaeI	CTAG	1 cut(s)	779
MaeII	ACGT	2 cut(s)	513, 745
MaeIII	GTNAC	5 cut(s)	74, 671, 768, 1491, 1694
MalI	GATC	7 cut(s)	350, 396, 789, 1509, 1599, 1625, 1702
MboI	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
MfeI	CAATTG	1 cut(s)	1731
MhlI	GDGCHC	3 cut(s)	1416, 1443, 1542
MlyI	GAGTC	2 cut(s)	859, 1352
MmeI	TCCRAC	1 cut(s)	145
Mph1103I	ATGCAT	1 cut(s)	986
MroXI	GAANNNNTTC	2 cut(s)	423, 1551
MseI	TTAA	6 cut(s)	579, 648, 845, 875, 1037, 1131
MslI	CAYNNNNRTG	2 cut(s)	65, 662
MspA1I	CMGCKG	2 cut(s)	698, 1115
MspCI	CTTAAG	1 cut(s)	647
MspI	CCGG	2 cut(s)	214, 1777
MspR9I	CCNGG	1 cut(s)	1182
MunI	CAATTG	1 cut(s)	1731
MvaI	CCWGG	1 cut(s)	1182
MvnI	CGCG	4 cut(s)	73, 85, 87, 146
MwoI	GCNNNNNNNGC	6 cut(s)	544, 707, 893, 1201, 1736, 1766
NdeII	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
NlaIV	GGNNCC	5 cut(s)	279, 1006, 1299, 1541, 1564
NmuCI	GTSAC	2 cut(s)	671, 1491
NsbI	TGCGCA	1 cut(s)	888
NsiI	ATGCAT	1 cut(s)	986
NspI	RCATGY	2 cut(s)	638, 1591
NspV	TTCGAA	1 cut(s)	525
PagI	TCATGA	2 cut(s)	753, 790
PauI	GCGCGC	1 cut(s)	85
PciSI	GCTCTTC	1 cut(s)	1448
PdmI	GAANNNNTTC	2 cut(s)	423, 1551
PfeI	GAWTC	2 cut(s)	522, 1608
PinAI	ACCGGT	1 cut(s)	1776
PkrI	GCNGC	6 cut(s)	256, 328, 633, 700, 953, 1762
PleI	GAGTC	2 cut(s)	858, 1352
PpsI	GAGTC	2 cut(s)	858, 1352
Ppu21I	YACGTR	1 cut(s)	746
PshBI	ATTAAT	1 cut(s)	875
PsiI	TTATAA	1 cut(s)	966
Psp6I	CCWGG	1 cut(s)	1180
PspGI	CCWGG	1 cut(s)	1180
PspN4I	GGNNCC	5 cut(s)	279, 1006, 1299, 1541, 1564
PspPI	GGNCC	2 cut(s)	278, 1321
PstI	CTGCAG	1 cut(s)	1265
PteI	GCGCGC	1 cut(s)	85
PvuII	CAGCTG	1 cut(s)	1115
RsaI	GTAC	2 cut(s)	305, 1781
RsaNI	GTAC	2 cut(s)	304, 1780
RseI	CAYNNNNRTG	2 cut(s)	65, 662
SapI	GCTCTTC	1 cut(s)	1448
SaqAI	TTAA	6 cut(s)	579, 648, 845, 875, 1037, 1131
SatI	GCNGC	6 cut(s)	255, 327, 632, 699, 952, 1761
Sau3AI	GATC	7 cut(s)	348, 394, 787, 1507, 1597, 1623, 1700
Sau96I	GGNCC	2 cut(s)	278, 1321
SchI	GAGTC	2 cut(s)	859, 1352
ScrFI	CCNGG	1 cut(s)	1182
SduI	GDGCHC	3 cut(s)	1416, 1443, 1542
SfaNI	GCATC	5 cut(s)	381, 883, 1354, 1650, 1756
SfcI	CTRYAG	1 cut(s)	1261
SfuI	TTCGAA	1 cut(s)	525
SinI	GGWCC	2 cut(s)	278, 1321
SmiMI	CAYNNNNRTG	2 cut(s)	65, 662
SmlI	CTYRAG	3 cut(s)	647, 1055, 1664
SmoI	CTYRAG	3 cut(s)	647, 1055, 1664
SspMI	CTAG	1 cut(s)	779
StyD4I	CCNGG	1 cut(s)	1180
TaaI	ACNGT	4 cut(s)	182, 627, 1325, 1519
TaiI	ACGT	2 cut(s)	516, 748
TaqI	TCGA	5 cut(s)	351, 375, 525, 1615, 1656
TauI	GCSGC	3 cut(s)	329, 701, 1763
TfiI	GAWTC	2 cut(s)	522, 1608
Tru1I	TTAA	6 cut(s)	579, 648, 845, 875, 1037, 1131
Tru9I	TTAA	6 cut(s)	579, 648, 845, 875, 1037, 1131
TscAI	CASTG	4 cut(s)	486, 630, 1102, 1368
TseFI	GTSAC	2 cut(s)	671, 1491
TseI	GCWGC	3 cut(s)	254, 631, 951
Tsp45I	GTSAC	2 cut(s)	671, 1491
TspDTI	ATGAA	6 cut(s)	464, 556, 612, 1136, 1435, 1764
TspGWI	ACGGA	2 cut(s)	259, 1713
TspRI	CASTG	4 cut(s)	486, 630, 1102, 1368
Vha464I	CTTAAG	1 cut(s)	647
VpaK11BI	GGWCC	2 cut(s)	278, 1321
VspI	ATTAAT	1 cut(s)	875
XapI	RAATTY	5 cut(s)	910, 1125, 1172, 1230, 1243
XceI	RCATGY	2 cut(s)	638, 1591
XcmI	CCANNNNNNNNNTGG	1 cut(s)	664
XmnI	GAANNNNTTC	2 cut(s)	423, 1551
XspI	CTAG	1 cut(s)	779
Zsp2I	ATGCAT	1 cut(s)	986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.