Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Forward (+)

64668508 .. 64672541

Gene Structure 4034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG464100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGGCGCGTTGCGTCCCACGCGCCACCCTGATATCCACCGCGTCATTTCCGAAACTGGTCGTCCCTGGCAGAACGAATCGCGTCGAGGCCTCGAAATCTGCGCTATTCGGCCGGAAATTCCACTCGCTGTGCCGTTACGATCGGAGAGACGCTGCGAGGCCATTGGTGAGGACGCGAGTCTTCGCTGAGCAGAAGGAGAAGGAGAAGGAGCTCCAGGTCAATGTGAGCGTTAGCATCGAACAAGGTTTGCCTGAGGATCACGAAATATCGAGGATTACCGAATTGCTTAGGCTAAATGTTCGAACTGCATTGAACCTAGCGTTTGGTGGATTGAAAGATTCTGGTGATGGTTTTCAAAGCGCGGAGCTTTCGGTGATTCTGTGCAATGATGAGTTTATGAGAAAACGTAACAAGGAATGGAGAGACGAGGACCGTGCTACTGGTGTTGTTTCCAAGTCCAAGCATGTGTCTGGTCTTAAGATTCCAAATGTTGTGTTGGGTGACATTGTGATTTCTGTTGAGATGGCGGCGAGGCAAGCAGAGCAAAGAGGCCACACACTTCTTGATGAGATGCGCCTTCTCATGGTGCAGGGATTGTTACATTTATTGAGATTTGATCATGAGGGTAAAGAGGCTACAGGGGAAATGGAGAAGGAGGAGGAACGTCTTTTAGACAGTCTTGGGTGGAAGGGGAAAAGAATGGTTGCAGTTTCAGTTTCAGTTTCAGTTTCCGAAACCCATCCAAAATCCAACGAGGGGAAAAGATGGAAGAAAGAAGGCAGCCTCCGATTTTATAAACCAAAGTTCAAATATGTGTTCTGTGACATGGATGGAACCCTCCTCAACAGCAAAAGTCAATTGAGTTCAACCACTGCCAGGGCTCTCAAAGAAGCCTCATCAAGGGGTGTGAAAATAGTGATTGCCACCGGTAAAGCTCGTCCAGCTGTGATGCGCGTCTTTAAGATGGTAGATTTAGCTGGAAAAGATGGCATTGTTTCAGAATTTTCTCCTGGGGTTTACTTGCAGGGATTGCTGGTTTATGGTATTCAAGGTCAGGAAATTTGTAAAAAGAATTTAGATCCAAATGTCTGCAGAGAGGCATGTCTTTACTCATTGGAGAATAAGGTTCCTCTCATTGCATTCAGCAAGGACCGATGCTCGAGTCTGTTTGATCATCCATATGTTGATGCTATGCATACTGTATATCATGAGCCAAAGGCAGAGATCATGCCTTCAGTTGAGCAGTTCTTGGCTGCTGCTGACATACAGAAACTGGTCTTCATGGACACTGCTGAGGGAGTATCTACTGCTTTGAGGCCGTATTGGTCAGAAGCAACTGGAGATCGGGCCACAGTTGTTCAAACTGTGCCGGACATGCTTGAACTTGTTCCCCCAGGAACCTCAAAAGGCAGTGGAGTAAAGATGTTGCTTGATCATTTGGGAATCACTCCAAAGGAGATCATGGCTATTGGCGATGGGGAAAATGATGTTGAGATGCTTGAGCTGGCTGGTTTGGGCATTTGTCTCAGTAATGGAGCAGAAAAAACAAAAGCTGTGGCCAATGTAATCGGTGCCAGCAACGACGAAGATGGTGCGGCTGATGCAATCTACCGCTATGCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

541

Amino Acids

59.77

Weight (kDa)

7.96

Isoelectric Point (pI)

32.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	119 - 226	3.4e-25	Endoribonuclease YbeY
Hydrolase_3	PF08282	272 - 536	8.7e-50	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	444 - 504	1.3e-06	haloacid dehalogenase-like hydrolase
S6PP	PF05116	447 - 515	3.2e-08	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	795
AccB1I	GGYRCC	1 cut(s)	1571
AccII	CGCG	7 cut(s)	7, 21, 41, 81, 175, 362, 954
AciI	CCGC	5 cut(s)	39, 362, 527, 1595, 1612
AclWI	GGATC	2 cut(s)	264, 1073
AcoI	YGGCCR	2 cut(s)	109, 1557
AcsI	RAATTY	4 cut(s)	116, 1001, 1059, 1072
AcuI	CTGAAG	1 cut(s)	1218
AfiI	CCNNNNNNNGG	4 cut(s)	583, 756, 876, 900
AflII	CTTAAG	1 cut(s)	476
AgeI	ACCGGT	1 cut(s)	926
AgsI	TTSAA	8 cut(s)	313, 334, 356, 808, 867, 1049, 1361, 1382
AjnI	CCWGG	5 cut(s)	64, 213, 875, 1009, 1393
AluBI	AGCT	7 cut(s)	211, 367, 935, 944, 977, 1504, 1553
AluI	AGCT	7 cut(s)	211, 367, 935, 944, 977, 1504, 1553
Alw21I	GWGCWC	1 cut(s)	213
Alw26I	GTCTC	3 cut(s)	141, 417, 1529
AlwI	GGATC	2 cut(s)	264, 1073
AlwNI	CAGNNNCTG	1 cut(s)	1273
Ama87I	CYCGRG	1 cut(s)	1159
AoxI	GGCC	7 cut(s)	87, 109, 158, 550, 1316, 1347, 1557
ApeKI	GCWGC	4 cut(s)	152, 780, 1253, 1256
ApoI	RAATTY	4 cut(s)	116, 1001, 1059, 1072
AsiGI	ACCGGT	1 cut(s)	926
Asp700I	GAANNNNTTC	1 cut(s)	1386
AspLEI	GCGC	6 cut(s)	7, 23, 103, 362, 576, 954
AspS9I	GGNCC	3 cut(s)	430, 1150, 1347
AsuHPI	GGTGA	4 cut(s)	178, 356, 385, 512
AsuII	TTCGAA	1 cut(s)	301
AvaI	CYCGRG	1 cut(s)	1159
AvaII	GGWCC	2 cut(s)	430, 1150
AxyI	CCTNAGG	1 cut(s)	252
BalI	TGGCCA	1 cut(s)	1559
BanI	GGYRCC	1 cut(s)	1571
BanII	GRGCYC	2 cut(s)	213, 883
BbsI	GAAGAC	2 cut(s)	172, 1270
Bbv12I	GWGCWC	1 cut(s)	213
BbvCI	CCTCAGC	1 cut(s)	1293
BbvI	GCAGC	4 cut(s)	139, 792, 1240, 1243
BccI	CCATC	9 cut(s)	341, 517, 747, 759, 824, 958, 980, 1469, 1583
BceAI	ACGGC	2 cut(s)	117, 1303
BcgI	CGANNNNNNTGC	4 cut(s)	416, 450, 1574, 1608
BciT130I	CCWGG	5 cut(s)	66, 215, 877, 1011, 1395
BclI	TGATCA	3 cut(s)	616, 1171, 1432
BcoDI	GTCTC	3 cut(s)	141, 417, 1529
BfaI	CTAG	1 cut(s)	317
BfmI	CTRYAG	2 cut(s)	636, 1090
BfrI	CTTAAG	1 cut(s)	476
BisI	GCNGC	6 cut(s)	153, 528, 781, 1254, 1257, 1596
BlpI	GCTNAGC	1 cut(s)	186
BlsI	GCNGC	6 cut(s)	154, 529, 782, 1255, 1258, 1597
Bme1390I	CCNGG	5 cut(s)	66, 215, 877, 1011, 1395
Bme18I	GGWCC	2 cut(s)	430, 1150
BmeT110I	CYCGRG	1 cut(s)	1159
BmgT120I	GGNCC	3 cut(s)	430, 1150, 1347
BmiI	GGNNCC	4 cut(s)	835, 1128, 1399, 1573
BmrFI	CCNGG	5 cut(s)	66, 215, 877, 1011, 1395
BmsI	GCATC	7 cut(s)	243, 561, 939, 1145, 1177, 1485, 1591
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	2 cut(s)	172, 1270
BpmI	CTGGAG	2 cut(s)	197, 1359
Bpu10I	CCTNAGC	2 cut(s)	287, 1293
Bpu1102I	GCTNAGC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	301
BpuEI	CTTGAG	1 cut(s)	1520
BsaJI	CCNNGG	4 cut(s)	64, 876, 1010, 1393
BsaWI	WCCGGW	1 cut(s)	926
Bsc4I	CCNNNNNNNGG	4 cut(s)	583, 756, 876, 900
Bse118I	RCCGGY	1 cut(s)	926
Bse1I	ACTGG	4 cut(s)	60, 445, 1278, 1342
Bse21I	CCTNAGG	1 cut(s)	252
Bse3DI	GCAATG	2 cut(s)	391, 1134
BseBI	CCWGG	5 cut(s)	66, 215, 877, 1011, 1395
BseDI	CCNNGG	4 cut(s)	64, 876, 1010, 1393
BseGI	GGATG	3 cut(s)	739, 835, 1174
BseLI	CCNNNNNNNGG	4 cut(s)	583, 756, 876, 900
BseMI	GCAATG	2 cut(s)	391, 1134
BseMII	CTCAG	4 cut(s)	177, 243, 1284, 1540
BseNI	ACTGG	4 cut(s)	60, 445, 1278, 1342
BseRI	GAGGAG	2 cut(s)	671, 830
BseX3I	CGGCCG	1 cut(s)	109
BseXI	GCAGC	4 cut(s)	139, 792, 1240, 1243
BsgI	GTGCAG	1 cut(s)	608
Bsh1236I	CGCG	7 cut(s)	7, 21, 41, 81, 175, 362, 954
Bsh1285I	CGRYCG	2 cut(s)	112, 142
BshFI	GGCC	7 cut(s)	89, 111, 160, 552, 1318, 1349, 1559
BshNI	GGYRCC	1 cut(s)	1571
BshTI	ACCGGT	1 cut(s)	926
BsiEI	CGRYCG	2 cut(s)	112, 142
BsiHKAI	GWGCWC	1 cut(s)	213
BsiHKCI	CYCGRG	1 cut(s)	1159
BsiSI	CCGG	3 cut(s)	112, 927, 1370
BslFI	GGGAC	1 cut(s)	47
BslI	CCNNNNNNNGG	4 cut(s)	583, 756, 876, 900
BsmAI	GTCTC	3 cut(s)	141, 417, 1529
BsmBI	CGTCTC	2 cut(s)	141, 417
BsmFI	GGGAC	1 cut(s)	47
BsmI	GAATGC	1 cut(s)	1139
BsnI	GGCC	7 cut(s)	89, 111, 160, 552, 1318, 1349, 1559
BsoBI	CYCGRG	1 cut(s)	1159
Bsp119I	TTCGAA	1 cut(s)	301
Bsp1286I	GDGCHC	2 cut(s)	213, 883
Bsp143I	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
Bsp1720I	GCTNAGC	1 cut(s)	186
BspACI	CCGC	5 cut(s)	39, 362, 527, 1595, 1612
BspANI	GGCC	7 cut(s)	89, 111, 160, 552, 1318, 1349, 1559
BspCNI	CTCAG	4 cut(s)	178, 244, 1285, 1539
BspFNI	CGCG	7 cut(s)	7, 21, 41, 81, 175, 362, 954
BspHI	TCATGA	2 cut(s)	619, 1207
BspLI	GGNNCC	4 cut(s)	835, 1128, 1399, 1573
BspMAI	CTGCAG	1 cut(s)	1094
BspPI	GGATC	2 cut(s)	264, 1073
BspT104I	TTCGAA	1 cut(s)	301
BspT107I	GGYRCC	1 cut(s)	1571
BspTI	CTTAAG	1 cut(s)	476
BsrDI	GCAATG	2 cut(s)	391, 1134
BsrFI	RCCGGY	1 cut(s)	926
BsrI	ACTGG	4 cut(s)	60, 445, 1278, 1342
BssAI	RCCGGY	1 cut(s)	926
BssECI	CCNNGG	4 cut(s)	64, 876, 1010, 1393
BssMI	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
Bst2UI	CCWGG	5 cut(s)	66, 215, 877, 1011, 1395
Bst4CI	ACNGT	5 cut(s)	434, 677, 1201, 1354, 1366
BstAFI	CTTAAG	1 cut(s)	476
BstAPI	GCANNNNNTGC	1 cut(s)	1030
BstBI	TTCGAA	1 cut(s)	301
BstC8I	GCNNGC	3 cut(s)	537, 1506, 1576
BstDEI	CTNAG	5 cut(s)	186, 252, 287, 1293, 1526
BstENI	CCTNNNNNAGG	1 cut(s)	898
BstF5I	GGATG	3 cut(s)	739, 835, 1174
BstFNI	CGCG	7 cut(s)	7, 21, 41, 81, 175, 362, 954
BstHHI	GCGC	6 cut(s)	7, 23, 103, 362, 576, 954
BstKTI	GATC	9 cut(s)	142, 259, 619, 1081, 1174, 1227, 1345, 1435, 1461
BstMAI	GTCTC	3 cut(s)	141, 417, 1529
BstMBI	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
BstMCI	CGRYCG	2 cut(s)	112, 142
BstMWI	GCNNNNNNNGC	7 cut(s)	18, 536, 541, 941, 1030, 1375, 1601
BstNI	CCWGG	5 cut(s)	66, 215, 877, 1011, 1395
BstNSI	RCATGY	3 cut(s)	467, 1104, 1378
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSCI	CCNGG	5 cut(s)	64, 213, 875, 1009, 1393
BstSFI	CTRYAG	2 cut(s)	636, 1090
BstUI	CGCG	7 cut(s)	7, 21, 41, 81, 175, 362, 954
BstV1I	GCAGC	4 cut(s)	139, 792, 1240, 1243
BstV2I	GAAGAC	2 cut(s)	172, 1270
BstX2I	RGATCY	1 cut(s)	1078
BstYI	RGATCY	1 cut(s)	1078
BstZI	CGGCCG	1 cut(s)	109
Bsu36I	CCTNAGG	1 cut(s)	252
BsuRI	GGCC	7 cut(s)	89, 111, 160, 552, 1318, 1349, 1559
BtgZI	GCGATG	1 cut(s)	1488
BtsCI	GGATG	3 cut(s)	739, 835, 1174
BtsI	GCAGTG	3 cut(s)	870, 1287, 1417
BtsIMutI	CAGTG	3 cut(s)	870, 1287, 1417
Cac8I	GCNNGC	3 cut(s)	537, 1506, 1576
CaiI	CAGNNNCTG	1 cut(s)	1273
CciI	TCATGA	2 cut(s)	619, 1207
CfoI	GCGC	6 cut(s)	7, 23, 103, 362, 576, 954
Cfr10I	RCCGGY	1 cut(s)	926
Cfr13I	GGNCC	3 cut(s)	430, 1150, 1347
CseI	GACGC	5 cut(s)	30, 70, 158, 181, 943
CspAI	ACCGGT	1 cut(s)	926
CspCI	CAANNNNNGTGG	2 cut(s)	1536, 1571
DdeI	CTNAG	5 cut(s)	186, 252, 287, 1293, 1526
DpnI	GATC	9 cut(s)	141, 258, 618, 1080, 1173, 1226, 1344, 1434, 1460
DpnII	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
EaeI	YGGCCR	2 cut(s)	109, 1557
EagI	CGGCCG	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	211
EclXI	CGGCCG	1 cut(s)	109
Eco147I	AGGCCT	1 cut(s)	89
Eco24I	GRGCYC	2 cut(s)	213, 883
Eco32I	GATATC	1 cut(s)	33
Eco47I	GGWCC	2 cut(s)	430, 1150
Eco52I	CGGCCG	1 cut(s)	109
Eco53kI	GAGCTC	1 cut(s)	211
Eco57I	CTGAAG	1 cut(s)	1218
Eco81I	CCTNAGG	1 cut(s)	252
Eco88I	CYCGRG	1 cut(s)	1159
EcoICRI	GAGCTC	1 cut(s)	211
EcoNI	CCTNNNNNAGG	1 cut(s)	898
EcoRII	CCWGG	5 cut(s)	64, 213, 875, 1009, 1393
EcoRV	GATATC	1 cut(s)	33
EcoT22I	ATGCAT	1 cut(s)	1197
EcoT38I	GRGCYC	2 cut(s)	213, 883
Esp3I	CGTCTC	2 cut(s)	141, 417
FaqI	GGGAC	1 cut(s)	47
FauNDI	CATATG	1 cut(s)	1180
FbaI	TGATCA	3 cut(s)	616, 1171, 1432
Fnu4HI	GCNGC	6 cut(s)	153, 528, 781, 1254, 1257, 1596
FokI	GGATG	3 cut(s)	726, 842, 1161
FriOI	GRGCYC	2 cut(s)	213, 883
Fsp4HI	GCNGC	6 cut(s)	153, 528, 781, 1254, 1257, 1596
FspBI	CTAG	1 cut(s)	317
GlaI	GCGC	6 cut(s)	6, 22, 102, 361, 575, 953
GluI	GCNGC	6 cut(s)	153, 528, 781, 1254, 1257, 1596
GsuI	CTGGAG	2 cut(s)	197, 1359
HaeIII	GGCC	7 cut(s)	89, 111, 160, 552, 1318, 1349, 1559
HapII	CCGG	3 cut(s)	112, 927, 1370
HgaI	GACGC	5 cut(s)	30, 70, 158, 181, 943
HhaI	GCGC	6 cut(s)	7, 23, 103, 362, 576, 954
Hin6I	GCGC	6 cut(s)	5, 21, 101, 360, 574, 952
HinP1I	GCGC	6 cut(s)	5, 21, 101, 360, 574, 952
HinfI	GANTC	7 cut(s)	76, 177, 338, 376, 481, 1162, 1443
HpaII	CCGG	3 cut(s)	112, 927, 1370
HphI	GGTGA	4 cut(s)	178, 356, 385, 512
Hpy166II	GTNNAC	1 cut(s)	1018
Hpy188I	TCNGA	6 cut(s)	51, 144, 733, 788, 1000, 1330
Hpy188III	TCNNGA	5 cut(s)	260, 563, 620, 1055, 1208
Hpy8I	GTNNAC	1 cut(s)	1018
Hpy99I	CGWCG	2 cut(s)	86, 1586
HpyAV	CCTTC	8 cut(s)	187, 193, 199, 587, 646, 682, 770, 1242
HpyCH4III	ACNGT	5 cut(s)	434, 677, 1201, 1354, 1366
HpyCH4IV	ACGT	2 cut(s)	406, 664
HpyCH4V	TGCA	9 cut(s)	308, 384, 589, 707, 1024, 1092, 1139, 1195, 1604
HpyF10VI	GCNNNNNNNGC	7 cut(s)	18, 536, 541, 941, 1030, 1375, 1601
HpyF3I	CTNAG	5 cut(s)	186, 252, 287, 1293, 1526
HpySE526I	ACGT	2 cut(s)	406, 664
HspAI	GCGC	6 cut(s)	5, 21, 101, 360, 574, 952
Ksp22I	TGATCA	3 cut(s)	616, 1171, 1432
Kzo9I	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
LmnI	GCTCC	4 cut(s)	208, 216, 364, 1535
Lsp1109I	GCAGC	4 cut(s)	139, 792, 1240, 1243
LweI	GCATC	7 cut(s)	243, 561, 939, 1145, 1177, 1485, 1591
MaeI	CTAG	1 cut(s)	317
MaeII	ACGT	2 cut(s)	406, 664
MaeIII	GTNAC	5 cut(s)	134, 407, 500, 597, 821
MalI	GATC	9 cut(s)	141, 258, 618, 1080, 1173, 1226, 1344, 1434, 1460
MboI	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
MboII	GAAGA	4 cut(s)	172, 781, 1270, 1598
MfeI	CAATTG	1 cut(s)	857
MflI	RGATCY	1 cut(s)	1078
MhlI	GDGCHC	2 cut(s)	213, 883
MlsI	TGGCCA	1 cut(s)	1559
MluCI	AATT	6 cut(s)	116, 281, 857, 1001, 1059, 1072
MluNI	TGGCCA	1 cut(s)	1559
MlyI	GAGTC	2 cut(s)	186, 1171
MmeI	TCCRAC	1 cut(s)	774
Mox20I	TGGCCA	1 cut(s)	1559
Mph1103I	ATGCAT	1 cut(s)	1197
MroXI	GAANNNNTTC	1 cut(s)	1386
MscI	TGGCCA	1 cut(s)	1559
MseI	TTAA	2 cut(s)	477, 960
MslI	CAYNNNNRTG	1 cut(s)	1179
Msp20I	TGGCCA	1 cut(s)	1559
MspA1I	CMGCKG	1 cut(s)	944
MspCI	CTTAAG	1 cut(s)	476
MspI	CCGG	3 cut(s)	112, 927, 1370
MspR9I	CCNGG	5 cut(s)	66, 215, 877, 1011, 1395
MunI	CAATTG	1 cut(s)	857
Mva1269I	GAATGC	1 cut(s)	1139
MvaI	CCWGG	5 cut(s)	66, 215, 877, 1011, 1395
MvnI	CGCG	7 cut(s)	7, 21, 41, 81, 175, 362, 954
MwoI	GCNNNNNNNGC	7 cut(s)	18, 536, 541, 941, 1030, 1375, 1601
NdeI	CATATG	1 cut(s)	1180
NdeII	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
NlaIV	GGNNCC	4 cut(s)	835, 1128, 1399, 1573
NmuCI	GTSAC	2 cut(s)	500, 821
NsiI	ATGCAT	1 cut(s)	1197
NspI	RCATGY	3 cut(s)	467, 1104, 1378
NspV	TTCGAA	1 cut(s)	301
PaeR7I	CTCGAG	1 cut(s)	1159
PagI	TCATGA	2 cut(s)	619, 1207
PceI	AGGCCT	1 cut(s)	89
PctI	GAATGC	1 cut(s)	1139
PdmI	GAANNNNTTC	1 cut(s)	1386
PfeI	GAWTC	5 cut(s)	76, 338, 376, 481, 1443
PinAI	ACCGGT	1 cut(s)	926
PkrI	GCNGC	6 cut(s)	154, 529, 782, 1255, 1258, 1597
Ple19I	CGATCG	1 cut(s)	142
PleI	GAGTC	2 cut(s)	185, 1170
PpsI	GAGTC	2 cut(s)	185, 1170
PshAI	GACNNNNGTC	1 cut(s)	176
PsiI	TTATAA	1 cut(s)	795
Psp124BI	GAGCTC	1 cut(s)	213
Psp6I	CCWGG	5 cut(s)	64, 213, 875, 1009, 1393
PspGI	CCWGG	5 cut(s)	64, 213, 875, 1009, 1393
PspN4I	GGNNCC	4 cut(s)	835, 1128, 1399, 1573
PspPI	GGNCC	3 cut(s)	430, 1150, 1347
PspXI	VCTCGAGB	1 cut(s)	1159
PstI	CTGCAG	1 cut(s)	1094
PstNI	CAGNNNCTG	1 cut(s)	1273
PsuI	RGATCY	1 cut(s)	1078
PvuI	CGATCG	1 cut(s)	142
PvuII	CAGCTG	1 cut(s)	944
RseI	CAYNNNNRTG	1 cut(s)	1179
SacI	GAGCTC	1 cut(s)	213
SaqAI	TTAA	2 cut(s)	477, 960
SatI	GCNGC	6 cut(s)	153, 528, 781, 1254, 1257, 1596
Sau3AI	GATC	9 cut(s)	139, 256, 616, 1078, 1171, 1224, 1342, 1432, 1458
Sau96I	GGNCC	3 cut(s)	430, 1150, 1347
SchI	GAGTC	2 cut(s)	186, 1171
ScrFI	CCNGG	5 cut(s)	66, 215, 877, 1011, 1395
SduI	GDGCHC	2 cut(s)	213, 883
SfaNI	GCATC	7 cut(s)	243, 561, 939, 1145, 1177, 1485, 1591
SfcI	CTRYAG	2 cut(s)	636, 1090
Sfr274I	CTCGAG	1 cut(s)	1159
SfuI	TTCGAA	1 cut(s)	301
SinI	GGWCC	2 cut(s)	430, 1150
SlaI	CTCGAG	1 cut(s)	1159
SmiMI	CAYNNNNRTG	1 cut(s)	1179
SmlI	CTYRAG	3 cut(s)	476, 1159, 1499
SmoI	CTYRAG	3 cut(s)	476, 1159, 1499
Sse9I	AATT	6 cut(s)	116, 281, 857, 1001, 1059, 1072
SseBI	AGGCCT	1 cut(s)	89
SsiI	CCGC	5 cut(s)	39, 362, 527, 1595, 1612
SspMI	CTAG	1 cut(s)	317
SstI	GAGCTC	1 cut(s)	213
StuI	AGGCCT	1 cut(s)	89
StyD4I	CCNGG	5 cut(s)	64, 213, 875, 1009, 1393
TaaI	ACNGT	5 cut(s)	434, 677, 1201, 1354, 1366
TaiI	ACGT	2 cut(s)	409, 667
TaqI	TCGA	6 cut(s)	84, 92, 237, 269, 301, 1160
TaqII	GACCGA	1 cut(s)	1167
TasI	AATT	6 cut(s)	116, 281, 857, 1001, 1059, 1072
TauI	GCSGC	2 cut(s)	530, 1598
TfiI	GAWTC	5 cut(s)	76, 338, 376, 481, 1443
Tru1I	TTAA	2 cut(s)	477, 960
Tru9I	TTAA	2 cut(s)	477, 960
TscAI	CASTG	3 cut(s)	877, 1294, 1417
TseFI	GTSAC	2 cut(s)	500, 821
TseI	GCWGC	4 cut(s)	152, 780, 1253, 1256
Tsp45I	GTSAC	2 cut(s)	500, 821
TspDTI	ATGAA	1 cut(s)	1270
TspRI	CASTG	3 cut(s)	877, 1294, 1417
Vha464I	CTTAAG	1 cut(s)	476
VpaK11BI	GGWCC	2 cut(s)	430, 1150
XagI	CCTNNNNNAGG	1 cut(s)	898
XapI	RAATTY	4 cut(s)	116, 1001, 1059, 1072
XceI	RCATGY	3 cut(s)	467, 1104, 1378
XhoI	CTCGAG	1 cut(s)	1159
XmnI	GAANNNNTTC	1 cut(s)	1386
XspI	CTAG	1 cut(s)	317
Zsp2I	ATGCAT	1 cut(s)	1197

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.