RchiOBHm_Chr6g0306711

Uncharacterized protein family UPF0054

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003247
Physical Location & Seq
Forward (+)
65310513 .. 65315851
5339 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27573

Sequence Viewer

Length: 1995 bp
ATGATCCCATTATTATCTCTGTTAAAAAGAACTGCTAGTGATTCTGGCCACAAAGATGGAGTTATCATTCTTTTACCCTTCCAAAGAGCTTGTATCCATGGCCAGAAACTTGCATTACAACGGAGATTTTTTTTTATTTTTTTTAAAGAATTTAACTGTTTAACTACTATATACGCGAACCAACCAACCATTTCTTCCGTTCACGAGCTCGAAAGGCCTCTCTCTCTCTACATGCTCCTCCATCTCTCTACTCTCCTCCGCGCCATCCATTCTACCCGCCCAATGGCGCGTTACATCCCACGCGCCAGTCTCATATCCATCACCCCCATTCCCATCCGAAACACCACCCCCAGACCGAATTCAATCCTCCCTCCCCAACCGCACCGTTTCGACGCCTCCAAAGCCTCGCTCTTCTTCGGCCGGAGATTCCACGCCCTCTGCGGCGGCGACTGGAGAGCTCGTCCGAGACCGTCGTTACGCATCTGTGCTGCGCAGAGAGACTACCGGAAAGTGAGGCGGCGAGCCCCGAAGAGCAAGGACAAGGAGCTCGAGCTCAACGTCAGCATTTGCATCGAAGAAGAGTTGCCTGACGATCCTGAAATCCTTGGGATAGCGGAGTTGCTCAGGATTAATGTTCCGATGGCAATGAAGCTAGCGTTTGATGGCTTGAAAGATTCGGAGTATAAGACCAGGGACGATGGCATTAGTGACGTCGGAGGGTTTCAGAGCGTGGAGCTTTCGGTGATGCTCTGCAATGACGAGTTTATCCGAAAGCTTAACAAAGAATGGAGAGATGAGGACCATGCTACTGATGTGCTTTCAATGTCACAACATGTCCCTGAGCTTAGGCTTCCCATTCTTATGTTGGGTGACGTTGTGATTTCTGTTGAGACTGCGGCTAGACAAGCAGAAGAAAGAGGACATACGATTCTTGACGAGATACGTATTCTCATGATACATGGATTGCTGCATCTATTAGGATTTGATCATGAGATTAGCGAAGAAGCCGAAAAGGAAATGGAGAAGGAGGAGGAGCTTCTCTTGAAGAGCCTTGGGTGGACGGGAAAAGGATTGATTCAGAGTGCGTCCAGTGCTGAAATCAATGCAATTTCCCATGAGGAGAAACAGGATGACAGGAAGAGAGAAGGCAGCCTTCGATACTATAAACCAAAGTTCAAATATATATTCTGCGATATGGATGGCACCCTTCTCAACAGTAAAACTCAAATCAGTTCAACAACTGCAAAGGCTCTTAAAGAGGTCTCATCAAGGGGTGTGAAAATAGTGATAGCAACAGGAAAAACTCGCTCAGCTGTGATCAACATTTTTAAAATGGTAGACTTGGCAGGAAAAGATGGCATTGTTTCAGAATTTTCTACTGGGGTTTTCTGCCAGGGATTGCTTGTTTATGGTAAGCAAGGTCGGGAAATCTTTAAAGGGAATTTAGATCCAAATGTCTGCAGAGAGGCGTGTCTTTACTCTTGGGAGAATAGGATACCTCTTATTGCTTACAGTGAGGATCGTTGCTTCACTCTATTTGACCACCCACTAGTTGACTCACTACATACTGTATATCATGAGCCAAAGGGGGAGATCATGCCTTCAGTTGAGCATCTCTTGGCTGCTGGTGACATACAGAAAGTAATCTTCATGGACACTGCCGAAGGAGTCGCAACTACTTTGAGGCCATACTGGTCAGGAGCAATTAGAGATCGTGCAGCAGTTGTTCAAGCTCAGCCGGACATGCTTGAAATTGTCCCTCCGGGGACCTCAAAAGGGAGTGGTGTAAAATTGCTGCTTGATCATTTGGGCATCACTCCAAAGGAGATCATGGCTATTGGCGATGGAGAAAATGACATTGAGATGCTTGAACTAGCTTCTTTGGGTGTTGCTCTTAGCAATGGTTCAGAGAAAACAAAAGCTGTGGCTAATTTAATTGGTGCCAGTAACGACGAAGATGGTGCAGCTGATGCGATCTACCGGTATGCATTTTGA

Protein Analysis

664

Amino Acids

74.28

Weight (kDa)

6.29

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YbeY PF02130 242 - 350 1.2e-32 Endoribonuclease YbeY
Hydrolase_3 PF08282 395 - 659 2.5e-52 haloacid dehalogenase-like hydrolase
S6PP PF05116 575 - 638 5.4e-09 Sucrose-6F-phosphate phosphohydrolase
Hydrolase PF00702 584 - 626 3.6e-06 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 714
Acc16I TGCGCA 1 cut(s) 492
AccB1I GGYRCC 2 cut(s) 1202, 1940
AccI GTMKAC 1 cut(s) 1338
AccII CGCG 4 cut(s) 176, 261, 289, 303
AciI CCGC 8 cut(s) 259, 277, 380, 441, 444, 517, 614, 896
AclWI GGATC 3 cut(s) 587, 1442, 1527
AcoI YGGCCR 3 cut(s) 46, 100, 418
AcsI RAATTY 4 cut(s) 149, 358, 1370, 1441
AcuI CTGAAG 1 cut(s) 1587
AcyI GRCGYC 2 cut(s) 393, 711
AfiI CCNNNNNNNGG 2 cut(s) 283, 1776
AflIII ACRYGT 1 cut(s) 832
AgeI ACCGGT 1 cut(s) 1980
AgsI TTSAA 9 cut(s) 363, 670, 822, 1045, 1177, 1236, 1730, 1751, 1871
AhlI ACTAGT 1 cut(s) 1549
AjnI CCWGG 2 cut(s) 689, 1392
AjuI GAANNNNNNNTTGG 2 cut(s) 178, 210
Alw21I GWGCWC 4 cut(s) 210, 460, 549, 555
Alw26I GTCTC 5 cut(s) 314, 460, 492, 884, 1267
AlwI GGATC 3 cut(s) 587, 1442, 1527
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 5 cut(s) 46, 100, 215, 418, 1685
ApeKI GCWGC 7 cut(s) 488, 967, 1149, 1622, 1718, 1795, 1964
ApoI RAATTY 4 cut(s) 149, 358, 1370, 1441
AseI ATTAAT 1 cut(s) 630
AsiGI ACCGGT 1 cut(s) 1980
AspLEI GCGC 4 cut(s) 263, 289, 305, 493
AspS9I GGNCC 2 cut(s) 799, 1767
AsuC2I CCSGG 1 cut(s) 1764
AsuHPI GGTGA 4 cut(s) 313, 754, 881, 1640
AsuNHI GCTAGC 1 cut(s) 652
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 2 cut(s) 799, 1767
BalI TGGCCA 2 cut(s) 48, 102
BanI GGYRCC 2 cut(s) 1202, 1940
BanII GRGCYC 5 cut(s) 210, 460, 526, 549, 555
BauI CACGAG 1 cut(s) 203
Bbv12I GWGCWC 4 cut(s) 210, 460, 549, 555
BbvI GCAGC 7 cut(s) 475, 954, 1161, 1609, 1730, 1782, 1976
BcgI CGANNNNNNTGC 4 cut(s) 553, 587, 1943, 1977
BciT130I CCWGG 2 cut(s) 691, 1394
BciVI GTATCC 2 cut(s) 104, 1488
BclI TGATCA 3 cut(s) 985, 1317, 1801
BcnI CCSGG 1 cut(s) 1764
BcoDI GTCTC 5 cut(s) 314, 460, 492, 884, 1267
BcuI ACTAGT 1 cut(s) 1549
BfaI CTAG 5 cut(s) 36, 653, 900, 1550, 1874
BfmI CTRYAG 1 cut(s) 1459
BfuI GTATCC 2 cut(s) 104, 1488
BlpI GCTNAGC 2 cut(s) 1309, 1734
Bme1390I CCNGG 3 cut(s) 691, 1394, 1764
Bme18I GGWCC 2 cut(s) 799, 1767
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 799, 1767
BmiI GGNNCC 3 cut(s) 1204, 1768, 1942
BmrFI CCNGG 3 cut(s) 691, 1394, 1764
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 8 cut(s) 489, 579, 735, 979, 1621, 1821, 1854, 1960
BmtI GCTAGC 1 cut(s) 656
BmuI ACTGGG 1 cut(s) 1389
BpmI CTGGAG 1 cut(s) 472
Bpu10I CCTNAGC 3 cut(s) 623, 840, 845
Bpu1102I GCTNAGC 2 cut(s) 1309, 1734
BpuMI CCSGG 1 cut(s) 1764
BsaAI YACGTR 1 cut(s) 944
BsaHI GRCGYC 2 cut(s) 393, 711
BsaI GGTCTC 2 cut(s) 460, 1267
BsaJI CCNNGG 6 cut(s) 97, 604, 690, 1051, 1393, 1763
BsaWI WCCGGW 2 cut(s) 504, 1980
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 1776
Bse118I RCCGGY 1 cut(s) 1980
Bse1I ACTGG 6 cut(s) 306, 455, 1089, 1384, 1697, 1944
Bse3DI GCAATG 3 cut(s) 651, 760, 1906
BseBI CCWGG 2 cut(s) 691, 1394
BseDI CCNNGG 6 cut(s) 97, 604, 690, 1051, 1393, 1763
BseGI GGATG 5 cut(s) 264, 294, 333, 1135, 1204
BseLI CCNNNNNNNGG 2 cut(s) 283, 1776
BseMI GCAATG 3 cut(s) 651, 760, 1906
BseMII CTCAG 4 cut(s) 637, 831, 1323, 1748
BseNI ACTGG 6 cut(s) 306, 455, 1089, 1384, 1697, 1944
BseRI GAGGAG 5 cut(s) 227, 245, 1043, 1046, 1133
BseX3I CGGCCG 1 cut(s) 418
BseXI GCAGC 7 cut(s) 475, 954, 1161, 1609, 1730, 1782, 1976
BsgI GTGCAG 2 cut(s) 1737, 1983
Bsh1236I CGCG 4 cut(s) 176, 261, 289, 303
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 5 cut(s) 48, 102, 217, 420, 1687
BshNI GGYRCC 2 cut(s) 1202, 1940
BshTI ACCGGT 1 cut(s) 1980
BsiEI CGRYCG 1 cut(s) 421
BsiHKAI GWGCWC 4 cut(s) 210, 460, 549, 555
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 5 cut(s) 421, 505, 1739, 1763, 1981
BslFI GGGAC 4 cut(s) 707, 821, 1742, 1780
BslI CCNNNNNNNGG 2 cut(s) 283, 1776
BsmAI GTCTC 5 cut(s) 314, 460, 492, 884, 1267
BsmFI GGGAC 4 cut(s) 707, 821, 1742, 1780
BsnI GGCC 5 cut(s) 48, 102, 217, 420, 1687
Bso31I GGTCTC 2 cut(s) 460, 1267
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 5 cut(s) 210, 460, 526, 549, 555
Bsp1720I GCTNAGC 2 cut(s) 1309, 1734
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 8 cut(s) 259, 277, 380, 441, 444, 517, 614, 896
BspANI GGCC 5 cut(s) 48, 102, 217, 420, 1687
BspCNI CTCAG 4 cut(s) 636, 832, 1322, 1747
BspFNI CGCG 4 cut(s) 176, 261, 289, 303
BspHI TCATGA 3 cut(s) 951, 988, 1576
BspLI GGNNCC 3 cut(s) 1204, 1768, 1942
BspMAI CTGCAG 1 cut(s) 1463
BspOI GCTAGC 1 cut(s) 656
BspPI GGATC 3 cut(s) 587, 1442, 1527
BspQI GCTCTTC 3 cut(s) 416, 524, 1040
BspT107I GGYRCC 2 cut(s) 1202, 1940
BspTNI GGTCTC 2 cut(s) 460, 1267
BsrDI GCAATG 3 cut(s) 651, 760, 1906
BsrFI RCCGGY 1 cut(s) 1980
BsrI ACTGG 6 cut(s) 306, 455, 1089, 1384, 1697, 1944
BssAI RCCGGY 1 cut(s) 1980
BssECI CCNNGG 6 cut(s) 97, 604, 690, 1051, 1393, 1763
BssNI GRCGYC 2 cut(s) 393, 711
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 3 cut(s) 97, 604, 1051
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 2 cut(s) 691, 1394
Bst4CI ACNGT 6 cut(s) 158, 386, 471, 1217, 1514, 1570
Bst6I CTCTTC 5 cut(s) 416, 524, 573, 1040, 1133
BstACI GRCGYC 2 cut(s) 393, 711
BstAPI GCANNNNNTGC 1 cut(s) 1970
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 2 cut(s) 522, 654
BstDEI CTNAG 6 cut(s) 623, 840, 845, 1309, 1734, 1895
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 5 cut(s) 264, 294, 333, 1135, 1204
BstFNI CGCG 4 cut(s) 176, 261, 289, 303
BstHHI GCGC 4 cut(s) 263, 289, 305, 493
BstMAI GTCTC 5 cut(s) 314, 460, 492, 884, 1267
BstMCI CGRYCG 1 cut(s) 421
BstMWI GCNNNNNNNGC 6 cut(s) 214, 401, 905, 1091, 1744, 1970
BstNI CCWGG 2 cut(s) 691, 1394
BstNSI RCATGY 3 cut(s) 235, 836, 1747
BstSCI CCNGG 3 cut(s) 689, 1392, 1762
BstSFI CTRYAG 1 cut(s) 1459
BstSNI TACGTA 1 cut(s) 944
BstUI CGCG 4 cut(s) 176, 261, 289, 303
BstV1I GCAGC 7 cut(s) 475, 954, 1161, 1609, 1730, 1782, 1976
BstX2I RGATCY 1 cut(s) 1447
BstXI CCANNNNNNTGG 1 cut(s) 56
BstYI RGATCY 1 cut(s) 1447
BstZI CGGCCG 1 cut(s) 418
BsuI GTATCC 2 cut(s) 104, 1488
BsuRI GGCC 5 cut(s) 48, 102, 217, 420, 1687
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 1857
BtsCI GGATG 5 cut(s) 264, 294, 333, 1135, 1204
BtsI GCAGTG 1 cut(s) 1656
BtsIMutI CAGTG 3 cut(s) 1096, 1519, 1656
Cac8I GCNNGC 2 cut(s) 522, 654
CciI TCATGA 3 cut(s) 951, 988, 1576
CfoI GCGC 4 cut(s) 263, 289, 305, 493
Cfr10I RCCGGY 1 cut(s) 1980
Cfr13I GGNCC 2 cut(s) 799, 1767
CseI GACGC 2 cut(s) 401, 1074
CspAI ACCGGT 1 cut(s) 1980
CspCI CAANNNNNGTGG 2 cut(s) 1905, 1940
DdeI CTNAG 6 cut(s) 623, 840, 845, 1309, 1734, 1895
DraI TTTAAA 3 cut(s) 145, 1330, 1435
EaeI YGGCCR 3 cut(s) 46, 100, 418
EagI CGGCCG 1 cut(s) 418
Eam1104I CTCTTC 5 cut(s) 416, 524, 573, 1040, 1133
EarI CTCTTC 5 cut(s) 416, 524, 573, 1040, 1133
Ecl136II GAGCTC 4 cut(s) 208, 458, 547, 553
EclXI CGGCCG 1 cut(s) 418
Eco105I TACGTA 1 cut(s) 944
Eco130I CCWWGG 3 cut(s) 97, 604, 1051
Eco147I AGGCCT 1 cut(s) 217
Eco24I GRGCYC 5 cut(s) 210, 460, 526, 549, 555
Eco31I GGTCTC 2 cut(s) 460, 1267
Eco47I GGWCC 2 cut(s) 799, 1767
Eco52I CGGCCG 1 cut(s) 418
Eco53kI GAGCTC 4 cut(s) 208, 458, 547, 553
Eco57I CTGAAG 1 cut(s) 1587
Eco88I CYCGRG 1 cut(s) 548
EcoICRI GAGCTC 4 cut(s) 208, 458, 547, 553
EcoO109I RGGNCCY 1 cut(s) 1767
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 689, 1392
EcoT14I CCWWGG 3 cut(s) 97, 604, 1051
EcoT22I ATGCAT 1 cut(s) 1990
EcoT38I GRGCYC 5 cut(s) 210, 460, 526, 549, 555
ErhI CCWWGG 3 cut(s) 97, 604, 1051
FalI AAGNNNNNCTT 2 cut(s) 1137, 1169
FaqI GGGAC 4 cut(s) 707, 821, 1742, 1780
FauI CCCGC 1 cut(s) 284
FbaI TGATCA 3 cut(s) 985, 1317, 1801
FblI GTMKAC 1 cut(s) 1338
FokI GGATG 5 cut(s) 251, 281, 320, 1142, 1211
FriOI GRGCYC 5 cut(s) 210, 460, 526, 549, 555
FspBI CTAG 5 cut(s) 36, 653, 900, 1550, 1874
FspI TGCGCA 1 cut(s) 492
GlaI GCGC 4 cut(s) 262, 288, 304, 492
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 5 cut(s) 48, 102, 217, 420, 1687
HapII CCGG 5 cut(s) 421, 505, 1739, 1763, 1981
HgaI GACGC 2 cut(s) 401, 1074
HhaI GCGC 4 cut(s) 263, 289, 305, 493
Hin1I GRCGYC 2 cut(s) 393, 711
Hin6I GCGC 4 cut(s) 261, 287, 303, 491
HinP1I GCGC 4 cut(s) 261, 287, 303, 491
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HindIII AAGCTT 1 cut(s) 773
HinfI GANTC 7 cut(s) 41, 426, 674, 928, 1075, 1556, 1668
HpaII CCGG 5 cut(s) 421, 505, 1739, 1763, 1981
HphI GGTGA 4 cut(s) 313, 754, 881, 1640
Hpy166II GTNNAC 4 cut(s) 202, 1059, 1339, 1555
Hpy8I GTNNAC 4 cut(s) 202, 1059, 1339, 1555
Hpy99I CGWCG 4 cut(s) 395, 475, 716, 1955
HpyAV CCTTC 7 cut(s) 88, 1018, 1139, 1163, 1217, 1611, 1658
HpyCH4III ACNGT 6 cut(s) 158, 386, 471, 1217, 1514, 1570
HpyCH4IV ACGT 4 cut(s) 558, 711, 873, 943
HpyF10VI GCNNNNNNNGC 6 cut(s) 214, 401, 905, 1091, 1744, 1970
HpyF3I CTNAG 6 cut(s) 623, 840, 845, 1309, 1734, 1895
HpySE526I ACGT 4 cut(s) 558, 711, 873, 943
Hsp92I GRCGYC 2 cut(s) 393, 711
HspAI GCGC 4 cut(s) 261, 287, 303, 491
Ksp22I TGATCA 3 cut(s) 985, 1317, 1801
LguI GCTCTTC 3 cut(s) 416, 524, 1040
LmnI GCTCC 5 cut(s) 240, 544, 733, 1033, 1700
Lsp1109I GCAGC 7 cut(s) 475, 954, 1161, 1609, 1730, 1782, 1976
LweI GCATC 8 cut(s) 489, 579, 735, 979, 1621, 1821, 1854, 1960
MaeI CTAG 5 cut(s) 36, 653, 900, 1550, 1874
MaeII ACGT 4 cut(s) 558, 711, 873, 943
MaeIII GTNAC 7 cut(s) 290, 474, 707, 825, 869, 1628, 1946
MflI RGATCY 1 cut(s) 1447
MhlI GDGCHC 5 cut(s) 210, 460, 526, 549, 555
MlsI TGGCCA 2 cut(s) 48, 102
MluNI TGGCCA 2 cut(s) 48, 102
MlyI GAGTC 2 cut(s) 1550, 1677
MmeI TCCRAC 1 cut(s) 694
Mox20I TGGCCA 2 cut(s) 48, 102
Mph1103I ATGCAT 1 cut(s) 1990
MscI TGGCCA 2 cut(s) 48, 102
MslI CAYNNNNRTG 3 cut(s) 54, 860, 1862
Msp20I TGGCCA 2 cut(s) 48, 102
MspA1I CMGCKG 2 cut(s) 1313, 1967
MspI CCGG 5 cut(s) 421, 505, 1739, 1763, 1981
MspR9I CCNGG 3 cut(s) 691, 1394, 1764
MvaI CCWGG 2 cut(s) 691, 1394
MvnI CGCG 4 cut(s) 176, 261, 289, 303
MwoI GCNNNNNNNGC 6 cut(s) 214, 401, 905, 1091, 1744, 1970
NciI CCSGG 1 cut(s) 1764
NcoI CCATGG 1 cut(s) 97
NheI GCTAGC 1 cut(s) 652
NlaIV GGNNCC 3 cut(s) 1204, 1768, 1942
NmuCI GTSAC 4 cut(s) 707, 825, 869, 1628
NsbI TGCGCA 1 cut(s) 492
NsiI ATGCAT 1 cut(s) 1990
NspI RCATGY 3 cut(s) 235, 836, 1747
PaeR7I CTCGAG 1 cut(s) 548
PagI TCATGA 3 cut(s) 951, 988, 1576
PceI AGGCCT 1 cut(s) 217
PciI ACATGT 1 cut(s) 832
PciSI GCTCTTC 3 cut(s) 416, 524, 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PfeI GAWTC 5 cut(s) 41, 426, 674, 928, 1075
PinAI ACCGGT 1 cut(s) 1980
PleI GAGTC 2 cut(s) 1550, 1676
PpsI GAGTC 2 cut(s) 1550, 1676
Ppu21I YACGTR 1 cut(s) 944
PpuMI RGGWCCY 1 cut(s) 1767
PscI ACATGT 1 cut(s) 832
PshBI ATTAAT 1 cut(s) 630
Psp124BI GAGCTC 4 cut(s) 210, 460, 549, 555
Psp5II RGGWCCY 1 cut(s) 1767
Psp6I CCWGG 2 cut(s) 689, 1392
PspGI CCWGG 2 cut(s) 689, 1392
PspN4I GGNNCC 3 cut(s) 1204, 1768, 1942
PspPI GGNCC 2 cut(s) 799, 1767
PspPPI RGGWCCY 1 cut(s) 1767
PspXI VCTCGAGB 1 cut(s) 548
PstI CTGCAG 1 cut(s) 1463
PsuI RGATCY 1 cut(s) 1447
PvuII CAGCTG 2 cut(s) 1313, 1967
RseI CAYNNNNRTG 3 cut(s) 54, 860, 1862
SacI GAGCTC 4 cut(s) 210, 460, 549, 555
SapI GCTCTTC 3 cut(s) 416, 524, 1040
Sau96I GGNCC 2 cut(s) 799, 1767
SchI GAGTC 2 cut(s) 1550, 1677
ScrFI CCNGG 3 cut(s) 691, 1394, 1764
SduI GDGCHC 5 cut(s) 210, 460, 526, 549, 555
SfaNI GCATC 8 cut(s) 489, 579, 735, 979, 1621, 1821, 1854, 1960
SfcI CTRYAG 1 cut(s) 1459
Sfr274I CTCGAG 1 cut(s) 548
SinI GGWCC 2 cut(s) 799, 1767
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 3 cut(s) 54, 860, 1862
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SnaBI TACGTA 1 cut(s) 944
SpeI ACTAGT 1 cut(s) 1549
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 8 cut(s) 259, 277, 380, 441, 444, 517, 614, 896
SspMI CTAG 5 cut(s) 36, 653, 900, 1550, 1874
SstI GAGCTC 4 cut(s) 210, 460, 549, 555
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 3 cut(s) 689, 1392, 1762
StyI CCWWGG 3 cut(s) 97, 604, 1051
TaaI ACNGT 6 cut(s) 158, 386, 471, 1217, 1514, 1570
TaiI ACGT 4 cut(s) 561, 714, 876, 946
TaqI TCGA 5 cut(s) 210, 390, 549, 573, 1156
TaqII GACCGA 1 cut(s) 370
TauI GCSGC 4 cut(s) 444, 447, 520, 899
TfiI GAWTC 5 cut(s) 41, 426, 674, 928, 1075
TscAI CASTG 3 cut(s) 1096, 1519, 1663
TseFI GTSAC 4 cut(s) 707, 825, 869, 1628
TseI GCWGC 7 cut(s) 488, 967, 1149, 1622, 1718, 1795, 1964
Tsp45I GTSAC 4 cut(s) 707, 825, 869, 1628
TspDTI ATGAA 2 cut(s) 662, 1639
TspGWI ACGGA 2 cut(s) 136, 187
TspRI CASTG 3 cut(s) 1096, 1519, 1663
VpaK11BI GGWCC 2 cut(s) 799, 1767
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 4 cut(s) 149, 358, 1370, 1441
XceI RCATGY 3 cut(s) 235, 836, 1747
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XhoI CTCGAG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 1338
XspI CTAG 5 cut(s) 36, 653, 900, 1550, 1874
ZraI GACGTC 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 1990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.