Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Reverse (-)

9698661 .. 9702132

Gene Structure 3472 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1110300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGTATAAAACAAGGGACAATGCTATAGGCGGTGTTGCGGGATATGAAAGTGTGGAGCTTTCGGTGCTTCTTTGCTATAACGAGTTTATCGGGAAACTTAATAAGGAGTGGAGAGCGGAGGACAATGCTACTGATGTTATTTCCATGTCCCAACACGTCCCTGATCTTAAGCTTCCCATGCTTATGTTGGTGGACATTGTGATTTCTGTTGAGACAGTGGCAAGACAAGCGGAGCAAAGAGGCCATATGCTTCTTGACAAGATGCGCATTCTCATGGTACATGGATTGTTACATCTGTTAGGATTTGATCACAAGGTTAGTGAAGACACTGAAGTGGAAATGGAGGAGGAGGAGGAGGATCTTCTCTTAAAGAGTCTTGGGTGGAAGGGAAAAGGGTTAATTCAGAGTGCATATGATGCTGCGACCCATTCCGATGGGCTCAAACTAGGTGGCACCCTTCTCAACAGCAAAAGCCAACTGAGCTCAACAAATGTCAAGGCTATGAAAGAGGCCTCATCAAGGGCTGTGAAAATAGTGATAGCAACCGGAAAAGCTCGGCCAGCTGTGATTCGCGTTTTTAAGGGGGTAGATTTAACTGGAAAAGATGGCATTGTTTCAGAATTTTCTCCTGGGGTTTTCTTGCAGGGATTGCTTGTTTATGGTACACAAGGTCGGGAAATTTTTAGAAGGAATTTGGATACAGATGTATGCAGAGAGGCATGTCGTTACTCTCTGGAAAATGAGGTTCCTCTTATAACATTCACCAAGGATCATTGCTTATCCCTTTGTGATCACCCACTTGTTGATTCCATGCATACTGTTTATCACGAGCAAAAGGCGGAGATCATGCCTTCAGTTGAGCATCTCATGGCTGCTGCTGGTATACAGAAACTAGTCTTCATGGACACTGCTGAGGGAGTAACAACTGCTATGAGGCCATACTGGTCAGAAGCAAATGGAGATGGTGCCAGAGTTGTTCAAACCGTGCCTGACATGCTTGAAATTGTTCCCCCTGGAACCTCAAAAGGAAGTGGAGTGAGCATGCTGCTCGATCATTTGGGAATCACTCCAAAAGAGATCATGGCTATTGGCGATGGGGAAAATGATATCGAGATGCTTGAGCTGGCTTGCTTAGGCATTGCTCTTGGTAACGGAGTGGAGAAGACAAAAACTGTGGCTAACGTAATTGGTCTCAGCAACGACGAAAACGGCGTAGCTGATGCAATATACCGGTATGCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019203 GO:0042578 GO:0043167 GO:0043169 GO:0044237 GO:0046872 GO:0050308

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

415

Amino Acids

45.22

Weight (kDa)

5.09

Isoelectric Point (pI)

30.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	16 - 122	1.8e-27	Endoribonuclease YbeY
Hydrolase_3	PF08282	149 - 409	1.3e-45	haloacid dehalogenase-like hydrolase
S6PP	PF05116	322 - 388	3.6e-09	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	755
Acc16I	TGCGCA	1 cut(s)	266
AccB1I	GGYRCC	2 cut(s)	452, 965
AccBSI	CCGCTC	1 cut(s)	116
AccI	GTMKAC	1 cut(s)	883
AccII	CGCG	1 cut(s)	573
AciI	CCGC	5 cut(s)	30, 38, 116, 230, 839
AclWI	GGATC	2 cut(s)	366, 777
AcoI	YGGCCR	1 cut(s)	557
AcsI	RAATTY	3 cut(s)	620, 678, 691
AcuI	CTGAAG	2 cut(s)	351, 837
AfaI	GTAC	2 cut(s)	279, 664
AfiI	CCNNNNNNNGG	1 cut(s)	519
AflII	CTTAAG	1 cut(s)	167
AflIII	ACRYGT	1 cut(s)	154
AgeI	ACCGGT	1 cut(s)	1230
AgsI	TTSAA	2 cut(s)	980, 1001
AhlI	ACTAGT	1 cut(s)	892
AjiI	CACGTC	1 cut(s)	157
AjnI	CCWGG	2 cut(s)	628, 1012
AleI	CACNNNNGTG	1 cut(s)	332
AluBI	AGCT	7 cut(s)	58, 172, 483, 554, 563, 1123, 1217
AluI	AGCT	7 cut(s)	58, 172, 483, 554, 563, 1123, 1217
Alw21I	GWGCWC	1 cut(s)	485
Alw26I	GTCTC	2 cut(s)	206, 1196
AlwI	GGATC	2 cut(s)	366, 777
AoxI	GGCC	4 cut(s)	241, 510, 557, 935
ApeKI	GCWGC	4 cut(s)	419, 872, 875, 1045
ApoI	RAATTY	3 cut(s)	620, 678, 691
AsiGI	ACCGGT	1 cut(s)	1230
Asp700I	GAANNNNTTC	1 cut(s)	1005
AspLEI	GCGC	1 cut(s)	267
AsuHPI	GGTGA	2 cut(s)	754, 785
BaeI	ACNNNNGTAYC	2 cut(s)	690, 723
BanI	GGYRCC	2 cut(s)	452, 965
BanII	GRGCYC	2 cut(s)	441, 485
BauI	CACGAG	1 cut(s)	827
BbsI	GAAGAC	3 cut(s)	330, 889, 1169
Bbv12I	GWGCWC	1 cut(s)	485
BbvCI	CCTCAGC	1 cut(s)	912
BbvI	GCAGC	4 cut(s)	406, 859, 862, 1032
BccI	CCATC	4 cut(s)	428, 599, 956, 1088
BceAI	ACGGC	1 cut(s)	1225
BcgI	CGANNNNNNTGC	2 cut(s)	1030, 1064
BciT130I	CCWGG	2 cut(s)	630, 1014
BciVI	GTATCC	1 cut(s)	691
BclI	TGATCA	2 cut(s)	307, 790
BcoDI	GTCTC	2 cut(s)	206, 1196
BcuI	ACTAGT	1 cut(s)	892
BfaI	CTAG	2 cut(s)	446, 893
BfmI	CTRYAG	1 cut(s)	24
BfrI	CTTAAG	1 cut(s)	167
BfuI	GTATCC	1 cut(s)	691
BisI	GCNGC	4 cut(s)	420, 873, 876, 1046
BlsI	GCNGC	4 cut(s)	421, 874, 877, 1047
Bme1390I	CCNGG	2 cut(s)	630, 1014
BmgBI	CACGTC	1 cut(s)	157
BmiI	GGNNCC	4 cut(s)	454, 747, 967, 1018
BmrFI	CCNGG	2 cut(s)	630, 1014
BmsI	GCATC	5 cut(s)	252, 406, 871, 1104, 1210
BpiI	GAAGAC	3 cut(s)	330, 889, 1169
Bpu10I	CCTNAGC	2 cut(s)	912, 1132
BpuEI	CTTGAG	1 cut(s)	1139
BsaI	GGTCTC	1 cut(s)	1196
BsaJI	CCNNGG	3 cut(s)	629, 765, 1012
BsaWI	WCCGGW	2 cut(s)	545, 1230
Bsc4I	CCNNNNNNNGG	1 cut(s)	519
Bse118I	RCCGGY	1 cut(s)	1230
Bse1I	ACTGG	2 cut(s)	601, 947
Bse3DI	GCAATG	2 cut(s)	772, 1137
BseBI	CCWGG	2 cut(s)	630, 1014
BseDI	CCNNGG	3 cut(s)	629, 765, 1012
BseLI	CCNNNNNNNGG	1 cut(s)	519
BseMI	GCAATG	2 cut(s)	772, 1137
BseMII	CTCAG	3 cut(s)	470, 903, 1207
BseNI	ACTGG	2 cut(s)	601, 947
BseRI	GAGGAG	4 cut(s)	359, 362, 365, 368
BseXI	GCAGC	4 cut(s)	406, 859, 862, 1032
Bsh1236I	CGCG	1 cut(s)	573
BshFI	GGCC	4 cut(s)	243, 512, 559, 937
BshNI	GGYRCC	2 cut(s)	452, 965
BshTI	ACCGGT	1 cut(s)	1230
BsiHKAI	GWGCWC	1 cut(s)	485
BsiSI	CCGG	2 cut(s)	546, 1231
BslFI	GGGAC	3 cut(s)	29, 133, 143
BslI	CCNNNNNNNGG	1 cut(s)	519
BsmAI	GTCTC	2 cut(s)	206, 1196
BsmFI	GGGAC	3 cut(s)	29, 133, 143
BsmI	GAATGC	1 cut(s)	267
BsnI	GGCC	4 cut(s)	243, 512, 559, 937
Bso31I	GGTCTC	1 cut(s)	1196
Bsp1286I	GDGCHC	2 cut(s)	441, 485
Bsp143I	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
BspACI	CCGC	5 cut(s)	30, 38, 116, 230, 839
BspANI	GGCC	4 cut(s)	243, 512, 559, 937
BspCNI	CTCAG	3 cut(s)	471, 904, 1206
BspFNI	CGCG	1 cut(s)	573
BspLI	GGNNCC	4 cut(s)	454, 747, 967, 1018
BspPI	GGATC	2 cut(s)	366, 777
BspT107I	GGYRCC	2 cut(s)	452, 965
BspTI	CTTAAG	1 cut(s)	167
BspTNI	GGTCTC	1 cut(s)	1196
BsrBI	CCGCTC	1 cut(s)	116
BsrDI	GCAATG	2 cut(s)	772, 1137
BsrFI	RCCGGY	1 cut(s)	1230
BsrI	ACTGG	2 cut(s)	601, 947
BssAI	RCCGGY	1 cut(s)	1230
BssECI	CCNNGG	3 cut(s)	629, 765, 1012
BssMI	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
BssNAI	GTATAC	1 cut(s)	884
BssSI	CACGAG	1 cut(s)	827
BssT1I	CCWWGG	1 cut(s)	765
Bst1107I	GTATAC	1 cut(s)	884
Bst2BI	CACGAG	1 cut(s)	827
Bst2UI	CCWGG	2 cut(s)	630, 1014
Bst4CI	ACNGT	4 cut(s)	217, 820, 985, 1174
BstAFI	CTTAAG	1 cut(s)	167
BstAPI	GCANNNNNTGC	2 cut(s)	416, 649
BstC8I	GCNNGC	4 cut(s)	561, 1043, 1125, 1129
BstDEI	CTNAG	4 cut(s)	479, 912, 1132, 1193
BstENI	CCTNNNNNAGG	1 cut(s)	517
BstFNI	CGCG	1 cut(s)	573
BstHHI	GCGC	1 cut(s)	267
BstKTI	GATC	8 cut(s)	166, 310, 361, 772, 793, 846, 1054, 1080
BstMAI	GTCTC	2 cut(s)	206, 1196
BstMBI	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
BstMWI	GCNNNNNNNGC	8 cut(s)	64, 178, 227, 416, 480, 560, 649, 994
BstNI	CCWGG	2 cut(s)	630, 1014
BstNSI	RCATGY	3 cut(s)	723, 997, 1045
BstSCI	CCNGG	2 cut(s)	628, 1012
BstSFI	CTRYAG	1 cut(s)	24
BstUI	CGCG	1 cut(s)	573
BstV1I	GCAGC	4 cut(s)	406, 859, 862, 1032
BstV2I	GAAGAC	3 cut(s)	330, 889, 1169
BstX2I	RGATCY	1 cut(s)	358
BstXI	CCANNNNNNTGG	1 cut(s)	434
BstYI	RGATCY	1 cut(s)	358
BstZ17I	GTATAC	1 cut(s)	884
BsuI	GTATCC	1 cut(s)	691
BsuRI	GGCC	4 cut(s)	243, 512, 559, 937
BtgZI	GCGATG	1 cut(s)	1107
BtrI	CACGTC	1 cut(s)	157
BtsI	GCAGTG	1 cut(s)	906
BtsIMutI	CAGTG	3 cut(s)	222, 327, 906
Cac8I	GCNNGC	4 cut(s)	561, 1043, 1125, 1129
CfoI	GCGC	1 cut(s)	267
Cfr10I	RCCGGY	1 cut(s)	1230
Csp6I	GTAC	2 cut(s)	278, 663
CspAI	ACCGGT	1 cut(s)	1230
CspCI	CAANNNNNGTGG	4 cut(s)	430, 465, 1155, 1190
CviQI	GTAC	2 cut(s)	278, 663
DdeI	CTNAG	4 cut(s)	479, 912, 1132, 1193
DpnI	GATC	8 cut(s)	165, 309, 360, 771, 792, 845, 1053, 1079
DpnII	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
EaeI	YGGCCR	1 cut(s)	557
EciI	GGCGGA	1 cut(s)	854
Ecl136II	GAGCTC	1 cut(s)	483
Eco130I	CCWWGG	1 cut(s)	765
Eco147I	AGGCCT	1 cut(s)	512
Eco24I	GRGCYC	2 cut(s)	441, 485
Eco31I	GGTCTC	1 cut(s)	1196
Eco32I	GATATC	1 cut(s)	1108
Eco53kI	GAGCTC	1 cut(s)	483
Eco57I	CTGAAG	2 cut(s)	351, 837
EcoICRI	GAGCTC	1 cut(s)	483
EcoNI	CCTNNNNNAGG	1 cut(s)	517
EcoRII	CCWGG	2 cut(s)	628, 1012
EcoRV	GATATC	1 cut(s)	1108
EcoT14I	CCWWGG	1 cut(s)	765
EcoT22I	ATGCAT	2 cut(s)	816, 1240
EcoT38I	GRGCYC	2 cut(s)	441, 485
ErhI	CCWWGG	1 cut(s)	765
FaqI	GGGAC	3 cut(s)	29, 133, 143
FauI	CCCGC	1 cut(s)	31
FauNDI	CATATG	2 cut(s)	246, 412
FbaI	TGATCA	2 cut(s)	307, 790
FblI	GTMKAC	1 cut(s)	883
Fnu4HI	GCNGC	4 cut(s)	420, 873, 876, 1046
FriOI	GRGCYC	2 cut(s)	441, 485
Fsp4HI	GCNGC	4 cut(s)	420, 873, 876, 1046
FspAI	RTGCGCAY	1 cut(s)	266
FspBI	CTAG	2 cut(s)	446, 893
FspI	TGCGCA	1 cut(s)	266
GlaI	GCGC	1 cut(s)	266
GluI	GCNGC	4 cut(s)	420, 873, 876, 1046
HaeIII	GGCC	4 cut(s)	243, 512, 559, 937
HapII	CCGG	2 cut(s)	546, 1231
HhaI	GCGC	1 cut(s)	267
Hin6I	GCGC	1 cut(s)	265
HinP1I	GCGC	1 cut(s)	265
HindIII	AAGCTT	1 cut(s)	170
HinfI	GANTC	4 cut(s)	373, 568, 806, 1062
HpaII	CCGG	2 cut(s)	546, 1231
HphI	GGTGA	2 cut(s)	754, 785
Hpy166II	GTNNAC	3 cut(s)	193, 665, 884
Hpy188I	TCNGA	4 cut(s)	405, 433, 619, 949
Hpy188III	TCNNGA	6 cut(s)	91, 254, 674, 734, 827, 1111
Hpy8I	GTNNAC	3 cut(s)	193, 665, 884
Hpy99I	CGWCG	1 cut(s)	1205
HpyAV	CCTTC	4 cut(s)	379, 467, 681, 861
HpyCH4III	ACNGT	4 cut(s)	217, 820, 985, 1174
HpyCH4IV	ACGT	2 cut(s)	156, 1182
HpyCH4V	TGCA	6 cut(s)	410, 643, 711, 814, 1223, 1238
HpyF10VI	GCNNNNNNNGC	8 cut(s)	64, 178, 227, 416, 480, 560, 649, 994
HpyF3I	CTNAG	4 cut(s)	479, 912, 1132, 1193
HpySE526I	ACGT	2 cut(s)	156, 1182
HspAI	GCGC	1 cut(s)	265
Ksp22I	TGATCA	2 cut(s)	307, 790
Kzo9I	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
LmnI	GCTCC	2 cut(s)	55, 232
Lsp1109I	GCAGC	4 cut(s)	406, 859, 862, 1032
LweI	GCATC	5 cut(s)	252, 406, 871, 1104, 1210
MaeI	CTAG	2 cut(s)	446, 893
MaeII	ACGT	2 cut(s)	156, 1182
MaeIII	GTNAC	4 cut(s)	288, 725, 919, 1148
MalI	GATC	8 cut(s)	165, 309, 360, 771, 792, 845, 1053, 1079
MbiI	CCGCTC	1 cut(s)	116
MboI	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
MboII	GAAGA	4 cut(s)	335, 353, 889, 1174
MflI	RGATCY	1 cut(s)	358
MhlI	GDGCHC	2 cut(s)	441, 485
MluCI	AATT	6 cut(s)	399, 620, 678, 691, 1002, 1185
MlyI	GAGTC	1 cut(s)	382
Mph1103I	ATGCAT	2 cut(s)	816, 1240
MroXI	GAANNNNTTC	1 cut(s)	1005
MseI	TTAA	6 cut(s)	99, 168, 368, 398, 579, 593
MslI	CAYNNNNRTG	4 cut(s)	182, 272, 332, 432
MspA1I	CMGCKG	1 cut(s)	563
MspCI	CTTAAG	1 cut(s)	167
MspI	CCGG	2 cut(s)	546, 1231
MspR9I	CCNGG	2 cut(s)	630, 1014
Mva1269I	GAATGC	1 cut(s)	267
MvaI	CCWGG	2 cut(s)	630, 1014
MvnI	CGCG	1 cut(s)	573
MwoI	GCNNNNNNNGC	8 cut(s)	64, 178, 227, 416, 480, 560, 649, 994
NdeI	CATATG	2 cut(s)	246, 412
NdeII	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
NlaIV	GGNNCC	4 cut(s)	454, 747, 967, 1018
NmeAIII	GCCGAG	1 cut(s)	535
NsbI	TGCGCA	1 cut(s)	266
NsiI	ATGCAT	2 cut(s)	816, 1240
NspI	RCATGY	3 cut(s)	723, 997, 1045
OliI	CACNNNNGTG	1 cut(s)	332
PaeI	GCATGC	1 cut(s)	1045
PceI	AGGCCT	1 cut(s)	512
PcsI	WCGNNNNNNNCGW	1 cut(s)	1209
PctI	GAATGC	1 cut(s)	267
PdmI	GAANNNNTTC	1 cut(s)	1005
PfeI	GAWTC	3 cut(s)	568, 806, 1062
PinAI	ACCGGT	1 cut(s)	1230
PkrI	GCNGC	4 cut(s)	421, 874, 877, 1047
PleI	GAGTC	1 cut(s)	381
PpsI	GAGTC	1 cut(s)	381
PsiI	TTATAA	1 cut(s)	755
Psp124BI	GAGCTC	1 cut(s)	485
Psp6I	CCWGG	2 cut(s)	628, 1012
PspGI	CCWGG	2 cut(s)	628, 1012
PspN4I	GGNNCC	4 cut(s)	454, 747, 967, 1018
PsuI	RGATCY	1 cut(s)	358
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	2 cut(s)	279, 664
RsaNI	GTAC	2 cut(s)	278, 663
RseI	CAYNNNNRTG	4 cut(s)	182, 272, 332, 432
SacI	GAGCTC	1 cut(s)	485
SaqAI	TTAA	6 cut(s)	99, 168, 368, 398, 579, 593
SatI	GCNGC	4 cut(s)	420, 873, 876, 1046
Sau3AI	GATC	8 cut(s)	163, 307, 358, 769, 790, 843, 1051, 1077
SchI	GAGTC	1 cut(s)	382
ScrFI	CCNGG	2 cut(s)	630, 1014
SduI	GDGCHC	2 cut(s)	441, 485
SfaNI	GCATC	5 cut(s)	252, 406, 871, 1104, 1210
SfcI	CTRYAG	1 cut(s)	24
SmiMI	CAYNNNNRTG	4 cut(s)	182, 272, 332, 432
SmlI	CTYRAG	2 cut(s)	167, 1118
SmoI	CTYRAG	2 cut(s)	167, 1118
SpeI	ACTAGT	1 cut(s)	892
SphI	GCATGC	1 cut(s)	1045
Sse9I	AATT	6 cut(s)	399, 620, 678, 691, 1002, 1185
SseBI	AGGCCT	1 cut(s)	512
SsiI	CCGC	5 cut(s)	30, 38, 116, 230, 839
SspMI	CTAG	2 cut(s)	446, 893
SstI	GAGCTC	1 cut(s)	485
StuI	AGGCCT	1 cut(s)	512
StyD4I	CCNGG	2 cut(s)	628, 1012
StyI	CCWWGG	1 cut(s)	765
TaaI	ACNGT	4 cut(s)	217, 820, 985, 1174
TaiI	ACGT	2 cut(s)	159, 1185
TaqI	TCGA	2 cut(s)	1050, 1110
TasI	AATT	6 cut(s)	399, 620, 678, 691, 1002, 1185
TfiI	GAWTC	3 cut(s)	568, 806, 1062
Tru1I	TTAA	6 cut(s)	99, 168, 368, 398, 579, 593
Tru9I	TTAA	6 cut(s)	99, 168, 368, 398, 579, 593
TscAI	CASTG	3 cut(s)	222, 334, 913
TseI	GCWGC	4 cut(s)	419, 872, 875, 1045
TspDTI	ATGAA	3 cut(s)	60, 518, 889
TspGWI	ACGGA	1 cut(s)	1167
TspRI	CASTG	3 cut(s)	222, 334, 913
Vha464I	CTTAAG	1 cut(s)	167
XagI	CCTNNNNNAGG	1 cut(s)	517
XapI	RAATTY	3 cut(s)	620, 678, 691
XceI	RCATGY	3 cut(s)	723, 997, 1045
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XmiI	GTMKAC	1 cut(s)	883
XmnI	GAANNNNTTC	1 cut(s)	1005
XspI	CTAG	2 cut(s)	446, 893
Zsp2I	ATGCAT	2 cut(s)	816, 1240

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.