Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Reverse (-)

4238094 .. 4241870

Gene Structure 3777 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010772

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGGCGCGTTGCGTCCCACGCGCCACCCTGATATCCACAGCGTCATTTCCGAAACTGGTCGTCCCTGGCAGAACGAATCGCGTCGAAGCCTCGAAATCTGAGCTATTCGGCCGGAAATTCCACGCGCTGTGCAGTTACGATCGGAGAGACGCTTCGAGGCCATTGGTGAGGACGCGAGTCCTCGCTGAGCAGAAGGAGAAGGAGCTCCAGGTCAATGTGAGCGTTAGCATCGAACAAGGTTTGCCTGAGGATCACGAAATATCGAGGATTGCCGAATTGCTTAGGCTAAATGTTCGAACTGCATTGAACCTGGTGTTTGGTGGATCGAAAGATTCCGGTGGTGGTTTTGGAAGCGCGGAGCTTTCGGTGATTCTGTGCAATGATGAGTTTATGAGAAAACGTAACAAGGAATGGAGAGACGAGGACCGTGCTACTGGTGTTGTTTCCAAGTCCAAGCATGTGCAGGGATTGTTACATTTATTGAGATATGATCATGAGATCAGTAAAGAGGCTACAGGGGAAATGGAGAAGGAGGAGGAACGTCTTTTAGACAGTCTTGGGTGGAAGGGGAAAAAAATGGTTGCATCTGATGCTGAAACCCATCCAAAAGCCAACGAGGGGAAAAGATGGAAGAAAGAAGGCAGCCTCCGACTCCATAAACCAAAGTTCAAATATGTGTTCTGTGACATGGATGGAACCCTACTCAACAGCAAAAGTCAATTGAGTTCAACCACTGCCAGGGCTCTCAAAGAAGCCTCATCAAGGGGTGTGAAAATAGTGATTGCCACGGGTAAAGCTCGTCCAGCTGTGATGCGCGTCTTTAAGATGGTAGATTTAGCTGGAAAAGATGGCATTGTTTCAGAATTTTCTCCTGGGGTTTACTTGCAGGGATTGCTGGTTTATGGTATTCAAGGTCAGGAAATTTGTAAAAGGAATTTAGATCCACATGTCTGCAGAGAGGCATGTCTTTACTCATTGGAGAATATGGTTCCTCTCATTGCATTCAGCAAGGACCGGTGCTCGAGTCTGTTTGATCACCCATATGTTGATGCTATGCATACTGTATATCATGAGCCAAAGGCAGAGATCATGCCTTCAGTTGAGCAGTTCTTGGCTGCTGCTGACATACAGAAACTGGTCTTCATGGACACTGCTGAGGGAGTATCTACTGCTCTGAGGCCGTATTGGTCAGAAGCAACTGGAGATCGTGCCACAGTTGTTCAAACTGTGCCGGACATGCTTGAACTTGTTCCCCCAGGAACCTCAAAAGGTAGTGGAGTAAAGATGTTGCTTGATCATTTGGGAATCACTCCAAAGGAGATCATGGCTATTGGCGATGGGGAGAATGATGTTGAGATGCTTGAGCTGGCTGGTTTGGGCATTTGTCTCAGTAATGGATCAGAGAAAACAAAAGCTGTGGCGAATGTAATCGGTGCCAGCAACGACGAAGATGGTGCTGCTGATGCAATCTACCGCTATGCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

496

Amino Acids

54.54

Weight (kDa)

7.18

Isoelectric Point (pI)

36.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_3	PF08282	226 - 490	1.7e-49	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	398 - 458	1.1e-06	haloacid dehalogenase-like hydrolase
S6PP	PF05116	401 - 469	2e-08	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1433
AccII	CGCG	7 cut(s)	7, 21, 81, 125, 175, 356, 816
AciI	CCGC	2 cut(s)	356, 1474
AclWI	GGATC	4 cut(s)	258, 331, 935, 1405
AcoI	YGGCCR	1 cut(s)	109
AcsI	RAATTY	4 cut(s)	116, 863, 921, 934
AcuI	CTGAAG	1 cut(s)	1080
AfiI	CCNNNNNNNGG	3 cut(s)	618, 738, 762
AflIII	ACRYGT	1 cut(s)	946
AgeI	ACCGGT	1 cut(s)	1014
AgsI	TTSAA	6 cut(s)	307, 670, 729, 911, 1223, 1244
AjnI	CCWGG	6 cut(s)	64, 207, 309, 737, 871, 1255
AluBI	AGCT	8 cut(s)	103, 205, 361, 797, 806, 839, 1366, 1415
AluI	AGCT	8 cut(s)	103, 205, 361, 797, 806, 839, 1366, 1415
Alw21I	GWGCWC	2 cut(s)	207, 1022
Alw26I	GTCTC	3 cut(s)	141, 411, 1391
AlwI	GGATC	4 cut(s)	258, 331, 935, 1405
AlwNI	CAGNNNCTG	1 cut(s)	1135
Ama87I	CYCGRG	1 cut(s)	1021
AoxI	GGCC	3 cut(s)	109, 158, 1178
ApeKI	GCWGC	4 cut(s)	642, 1115, 1118, 1457
ApoI	RAATTY	4 cut(s)	116, 863, 921, 934
AsiGI	ACCGGT	1 cut(s)	1014
Asp700I	GAANNNNTTC	1 cut(s)	1248
AspLEI	GCGC	5 cut(s)	7, 23, 127, 356, 816
AspS9I	GGNCC	2 cut(s)	424, 1012
AsuHPI	GGTGA	3 cut(s)	178, 379, 1028
AsuII	TTCGAA	1 cut(s)	295
AvaI	CYCGRG	1 cut(s)	1021
AvaII	GGWCC	2 cut(s)	424, 1012
AxyI	CCTNAGG	1 cut(s)	246
BanI	GGYRCC	1 cut(s)	1433
BanII	GRGCYC	2 cut(s)	207, 745
BbsI	GAAGAC	1 cut(s)	1132
Bbv12I	GWGCWC	2 cut(s)	207, 1022
BbvCI	CCTCAGC	1 cut(s)	1155
BbvI	GCAGC	4 cut(s)	654, 1102, 1105, 1444
BccI	CCATC	7 cut(s)	609, 621, 686, 820, 842, 1331, 1445
BceAI	ACGGC	1 cut(s)	1165
BcgI	CGANNNNNNTGC	4 cut(s)	410, 444, 1436, 1470
BciT130I	CCWGG	6 cut(s)	66, 209, 311, 739, 873, 1257
BclI	TGATCA	3 cut(s)	490, 1033, 1294
BcoDI	GTCTC	3 cut(s)	141, 411, 1391
BfmI	CTRYAG	2 cut(s)	513, 952
BisI	GCNGC	4 cut(s)	643, 1116, 1119, 1458
BlpI	GCTNAGC	1 cut(s)	186
BlsI	GCNGC	4 cut(s)	644, 1117, 1120, 1459
Bme1390I	CCNGG	6 cut(s)	66, 209, 311, 739, 873, 1257
Bme18I	GGWCC	2 cut(s)	424, 1012
BmeT110I	CYCGRG	1 cut(s)	1021
BmgT120I	GGNCC	2 cut(s)	424, 1012
BmiI	GGNNCC	4 cut(s)	697, 990, 1261, 1435
BmrFI	CCNGG	6 cut(s)	66, 209, 311, 739, 873, 1257
BmsI	GCATC	7 cut(s)	237, 580, 593, 801, 1039, 1347, 1453
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	1 cut(s)	1132
BpmI	CTGGAG	2 cut(s)	191, 1221
Bpu10I	CCTNAGC	2 cut(s)	281, 1155
Bpu1102I	GCTNAGC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	295
BpuEI	CTTGAG	1 cut(s)	1382
BsaJI	CCNNGG	5 cut(s)	64, 738, 786, 872, 1255
BsaWI	WCCGGW	2 cut(s)	335, 1014
Bsc4I	CCNNNNNNNGG	3 cut(s)	618, 738, 762
Bse118I	RCCGGY	1 cut(s)	1014
Bse1I	ACTGG	4 cut(s)	60, 439, 1140, 1204
Bse21I	CCTNAGG	1 cut(s)	246
Bse3DI	GCAATG	2 cut(s)	385, 996
BseBI	CCWGG	6 cut(s)	66, 209, 311, 739, 873, 1257
BseDI	CCNNGG	5 cut(s)	64, 738, 786, 872, 1255
BseGI	GGATG	2 cut(s)	601, 697
BseLI	CCNNNNNNNGG	3 cut(s)	618, 738, 762
BseMI	GCAATG	2 cut(s)	385, 996
BseMII	CTCAG	6 cut(s)	90, 177, 237, 1146, 1166, 1402
BseNI	ACTGG	4 cut(s)	60, 439, 1140, 1204
BseRI	GAGGAG	1 cut(s)	548
BseX3I	CGGCCG	1 cut(s)	109
BseXI	GCAGC	4 cut(s)	654, 1102, 1105, 1444
BsgI	GTGCAG	2 cut(s)	151, 482
Bsh1236I	CGCG	7 cut(s)	7, 21, 81, 125, 175, 356, 816
Bsh1285I	CGRYCG	2 cut(s)	112, 142
BshFI	GGCC	3 cut(s)	111, 160, 1180
BshNI	GGYRCC	1 cut(s)	1433
BshTI	ACCGGT	1 cut(s)	1014
BsiEI	CGRYCG	2 cut(s)	112, 142
BsiHKAI	GWGCWC	2 cut(s)	207, 1022
BsiHKCI	CYCGRG	1 cut(s)	1021
BsiSI	CCGG	4 cut(s)	112, 336, 1015, 1232
BslFI	GGGAC	1 cut(s)	47
BslI	CCNNNNNNNGG	3 cut(s)	618, 738, 762
BsmAI	GTCTC	3 cut(s)	141, 411, 1391
BsmBI	CGTCTC	2 cut(s)	141, 411
BsmFI	GGGAC	1 cut(s)	47
BsmI	GAATGC	1 cut(s)	1001
BsnI	GGCC	3 cut(s)	111, 160, 1180
BsoBI	CYCGRG	1 cut(s)	1021
Bsp119I	TTCGAA	1 cut(s)	295
Bsp1286I	GDGCHC	3 cut(s)	207, 745, 1022
Bsp1720I	GCTNAGC	1 cut(s)	186
BspACI	CCGC	2 cut(s)	356, 1474
BspANI	GGCC	3 cut(s)	111, 160, 1180
BspCNI	CTCAG	6 cut(s)	91, 178, 238, 1147, 1167, 1401
BspFNI	CGCG	7 cut(s)	7, 21, 81, 125, 175, 356, 816
BspHI	TCATGA	2 cut(s)	493, 1069
BspLI	GGNNCC	4 cut(s)	697, 990, 1261, 1435
BspMAI	CTGCAG	1 cut(s)	956
BspPI	GGATC	4 cut(s)	258, 331, 935, 1405
BspT104I	TTCGAA	1 cut(s)	295
BspT107I	GGYRCC	1 cut(s)	1433
BsrDI	GCAATG	2 cut(s)	385, 996
BsrFI	RCCGGY	1 cut(s)	1014
BsrI	ACTGG	4 cut(s)	60, 439, 1140, 1204
BssAI	RCCGGY	1 cut(s)	1014
BssECI	CCNNGG	5 cut(s)	64, 738, 786, 872, 1255
Bst2UI	CCWGG	6 cut(s)	66, 209, 311, 739, 873, 1257
Bst4CI	ACNGT	5 cut(s)	428, 554, 1063, 1216, 1228
BstAPI	GCANNNNNTGC	2 cut(s)	590, 892
BstBI	TTCGAA	1 cut(s)	295
BstC8I	GCNNGC	2 cut(s)	1368, 1438
BstDEI	CTNAG	7 cut(s)	99, 186, 246, 281, 1155, 1175, 1388
BstDSI	CCRYGG	1 cut(s)	786
BstENI	CCTNNNNNAGG	1 cut(s)	760
BstF5I	GGATG	2 cut(s)	601, 697
BstFNI	CGCG	7 cut(s)	7, 21, 81, 125, 175, 356, 816
BstHHI	GCGC	5 cut(s)	7, 23, 127, 356, 816
BstMAI	GTCTC	3 cut(s)	141, 411, 1391
BstMCI	CGRYCG	2 cut(s)	112, 142
BstMWI	GCNNNNNNNGC	6 cut(s)	18, 590, 803, 892, 1237, 1463
BstNI	CCWGG	6 cut(s)	66, 209, 311, 739, 873, 1257
BstNSI	RCATGY	4 cut(s)	461, 950, 966, 1240
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSCI	CCNGG	6 cut(s)	64, 207, 309, 737, 871, 1255
BstSFI	CTRYAG	2 cut(s)	513, 952
BstUI	CGCG	7 cut(s)	7, 21, 81, 125, 175, 356, 816
BstV1I	GCAGC	4 cut(s)	654, 1102, 1105, 1444
BstV2I	GAAGAC	1 cut(s)	1132
BstX2I	RGATCY	1 cut(s)	940
BstYI	RGATCY	1 cut(s)	940
BstZI	CGGCCG	1 cut(s)	109
Bsu36I	CCTNAGG	1 cut(s)	246
BsuRI	GGCC	3 cut(s)	111, 160, 1180
BtgI	CCRYGG	1 cut(s)	786
BtgZI	GCGATG	1 cut(s)	1350
BtsCI	GGATG	2 cut(s)	601, 697
BtsI	GCAGTG	2 cut(s)	732, 1149
BtsIMutI	CAGTG	2 cut(s)	732, 1149
Cac8I	GCNNGC	2 cut(s)	1368, 1438
CaiI	CAGNNNCTG	1 cut(s)	1135
CciI	TCATGA	2 cut(s)	493, 1069
CfoI	GCGC	5 cut(s)	7, 23, 127, 356, 816
Cfr10I	RCCGGY	1 cut(s)	1014
Cfr13I	GGNCC	2 cut(s)	424, 1012
CseI	GACGC	5 cut(s)	30, 70, 158, 181, 805
CsiI	ACCWGGT	1 cut(s)	309
CspAI	ACCGGT	1 cut(s)	1014
CspCI	CAANNNNNGTGG	2 cut(s)	1398, 1433
DdeI	CTNAG	7 cut(s)	99, 186, 246, 281, 1155, 1175, 1388
EaeI	YGGCCR	1 cut(s)	109
EagI	CGGCCG	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	205
EclXI	CGGCCG	1 cut(s)	109
Eco24I	GRGCYC	2 cut(s)	207, 745
Eco32I	GATATC	1 cut(s)	33
Eco47I	GGWCC	2 cut(s)	424, 1012
Eco52I	CGGCCG	1 cut(s)	109
Eco53kI	GAGCTC	1 cut(s)	205
Eco57I	CTGAAG	1 cut(s)	1080
Eco81I	CCTNAGG	1 cut(s)	246
Eco88I	CYCGRG	1 cut(s)	1021
EcoICRI	GAGCTC	1 cut(s)	205
EcoNI	CCTNNNNNAGG	1 cut(s)	760
EcoRII	CCWGG	6 cut(s)	64, 207, 309, 737, 871, 1255
EcoRV	GATATC	1 cut(s)	33
EcoT22I	ATGCAT	2 cut(s)	1059, 1483
EcoT38I	GRGCYC	2 cut(s)	207, 745
Esp3I	CGTCTC	2 cut(s)	141, 411
FaqI	GGGAC	1 cut(s)	47
FauNDI	CATATG	1 cut(s)	1042
FbaI	TGATCA	3 cut(s)	490, 1033, 1294
Fnu4HI	GCNGC	4 cut(s)	643, 1116, 1119, 1458
FokI	GGATG	2 cut(s)	588, 704
FriOI	GRGCYC	2 cut(s)	207, 745
Fsp4HI	GCNGC	4 cut(s)	643, 1116, 1119, 1458
GlaI	GCGC	5 cut(s)	6, 22, 126, 355, 815
GluI	GCNGC	4 cut(s)	643, 1116, 1119, 1458
GsuI	CTGGAG	2 cut(s)	191, 1221
HaeIII	GGCC	3 cut(s)	111, 160, 1180
HapII	CCGG	4 cut(s)	112, 336, 1015, 1232
HgaI	GACGC	5 cut(s)	30, 70, 158, 181, 805
HhaI	GCGC	5 cut(s)	7, 23, 127, 356, 816
Hin6I	GCGC	5 cut(s)	5, 21, 125, 354, 814
HinP1I	GCGC	5 cut(s)	5, 21, 125, 354, 814
HinfI	GANTC	7 cut(s)	76, 177, 332, 370, 651, 1024, 1305
HpaII	CCGG	4 cut(s)	112, 336, 1015, 1232
HphI	GGTGA	3 cut(s)	178, 379, 1028
Hpy166II	GTNNAC	1 cut(s)	880
Hpy188I	TCNGA	9 cut(s)	51, 100, 144, 589, 650, 862, 1176, 1192, 1402
Hpy188III	TCNNGA	4 cut(s)	254, 494, 917, 1070
Hpy8I	GTNNAC	1 cut(s)	880
Hpy99I	CGWCG	2 cut(s)	86, 1448
HpyAV	CCTTC	6 cut(s)	187, 193, 523, 559, 632, 1104
HpyCH4III	ACNGT	5 cut(s)	428, 554, 1063, 1216, 1228
HpyCH4IV	ACGT	2 cut(s)	400, 541
HpyF10VI	GCNNNNNNNGC	6 cut(s)	18, 590, 803, 892, 1237, 1463
HpyF3I	CTNAG	7 cut(s)	99, 186, 246, 281, 1155, 1175, 1388
HpySE526I	ACGT	2 cut(s)	400, 541
HspAI	GCGC	5 cut(s)	5, 21, 125, 354, 814
Ksp22I	TGATCA	3 cut(s)	490, 1033, 1294
LmnI	GCTCC	3 cut(s)	202, 210, 358
Lsp1109I	GCAGC	4 cut(s)	654, 1102, 1105, 1444
LweI	GCATC	7 cut(s)	237, 580, 593, 801, 1039, 1347, 1453
MabI	ACCWGGT	1 cut(s)	309
MaeII	ACGT	2 cut(s)	400, 541
MaeIII	GTNAC	4 cut(s)	134, 401, 471, 683
MboII	GAAGA	3 cut(s)	643, 1132, 1460
MfeI	CAATTG	1 cut(s)	719
MflI	RGATCY	1 cut(s)	940
MhlI	GDGCHC	3 cut(s)	207, 745, 1022
MluCI	AATT	6 cut(s)	116, 275, 719, 863, 921, 934
MlyI	GAGTC	3 cut(s)	186, 645, 1033
MmeI	TCCRAC	1 cut(s)	673
Mph1103I	ATGCAT	2 cut(s)	1059, 1483
MroXI	GAANNNNTTC	1 cut(s)	1248
MseI	TTAA	1 cut(s)	822
MslI	CAYNNNNRTG	1 cut(s)	1041
MspA1I	CMGCKG	1 cut(s)	806
MspI	CCGG	4 cut(s)	112, 336, 1015, 1232
MspR9I	CCNGG	6 cut(s)	66, 209, 311, 739, 873, 1257
MunI	CAATTG	1 cut(s)	719
Mva1269I	GAATGC	1 cut(s)	1001
MvaI	CCWGG	6 cut(s)	66, 209, 311, 739, 873, 1257
MvnI	CGCG	7 cut(s)	7, 21, 81, 125, 175, 356, 816
MwoI	GCNNNNNNNGC	6 cut(s)	18, 590, 803, 892, 1237, 1463
NdeI	CATATG	1 cut(s)	1042
NlaIV	GGNNCC	4 cut(s)	697, 990, 1261, 1435
NmuCI	GTSAC	1 cut(s)	683
NsiI	ATGCAT	2 cut(s)	1059, 1483
NspI	RCATGY	4 cut(s)	461, 950, 966, 1240
NspV	TTCGAA	1 cut(s)	295
PaeR7I	CTCGAG	1 cut(s)	1021
PagI	TCATGA	2 cut(s)	493, 1069
PciI	ACATGT	1 cut(s)	946
PctI	GAATGC	1 cut(s)	1001
PdmI	GAANNNNTTC	1 cut(s)	1248
PfeI	GAWTC	4 cut(s)	76, 332, 370, 1305
PinAI	ACCGGT	1 cut(s)	1014
PkrI	GCNGC	4 cut(s)	644, 1117, 1120, 1459
Ple19I	CGATCG	1 cut(s)	142
PleI	GAGTC	3 cut(s)	185, 645, 1032
PpsI	GAGTC	3 cut(s)	185, 645, 1032
PscI	ACATGT	1 cut(s)	946
PshAI	GACNNNNGTC	1 cut(s)	176
Psp124BI	GAGCTC	1 cut(s)	207
Psp6I	CCWGG	6 cut(s)	64, 207, 309, 737, 871, 1255
PspGI	CCWGG	6 cut(s)	64, 207, 309, 737, 871, 1255
PspN4I	GGNNCC	4 cut(s)	697, 990, 1261, 1435
PspPI	GGNCC	2 cut(s)	424, 1012
PspXI	VCTCGAGB	1 cut(s)	1021
PstI	CTGCAG	1 cut(s)	956
PstNI	CAGNNNCTG	1 cut(s)	1135
PsuI	RGATCY	1 cut(s)	940
PvuI	CGATCG	1 cut(s)	142
PvuII	CAGCTG	1 cut(s)	806
RseI	CAYNNNNRTG	1 cut(s)	1041
SacI	GAGCTC	1 cut(s)	207
SaqAI	TTAA	1 cut(s)	822
SatI	GCNGC	4 cut(s)	643, 1116, 1119, 1458
Sau96I	GGNCC	2 cut(s)	424, 1012
SchI	GAGTC	3 cut(s)	186, 645, 1033
ScrFI	CCNGG	6 cut(s)	66, 209, 311, 739, 873, 1257
SduI	GDGCHC	3 cut(s)	207, 745, 1022
SexAI	ACCWGGT	1 cut(s)	309
SfaNI	GCATC	7 cut(s)	237, 580, 593, 801, 1039, 1347, 1453
SfcI	CTRYAG	2 cut(s)	513, 952
Sfr274I	CTCGAG	1 cut(s)	1021
SfuI	TTCGAA	1 cut(s)	295
SinI	GGWCC	2 cut(s)	424, 1012
SlaI	CTCGAG	1 cut(s)	1021
SmiMI	CAYNNNNRTG	1 cut(s)	1041
SmlI	CTYRAG	2 cut(s)	1021, 1361
SmoI	CTYRAG	2 cut(s)	1021, 1361
Sse9I	AATT	6 cut(s)	116, 275, 719, 863, 921, 934
SsiI	CCGC	2 cut(s)	356, 1474
SstI	GAGCTC	1 cut(s)	207
StyD4I	CCNGG	6 cut(s)	64, 207, 309, 737, 871, 1255
TaaI	ACNGT	5 cut(s)	428, 554, 1063, 1216, 1228
TaiI	ACGT	2 cut(s)	403, 544
TaqI	TCGA	8 cut(s)	84, 92, 155, 231, 263, 295, 326, 1022
TasI	AATT	6 cut(s)	116, 275, 719, 863, 921, 934
TfiI	GAWTC	4 cut(s)	76, 332, 370, 1305
Tru1I	TTAA	1 cut(s)	822
Tru9I	TTAA	1 cut(s)	822
TscAI	CASTG	2 cut(s)	739, 1156
TseFI	GTSAC	1 cut(s)	683
TseI	GCWGC	4 cut(s)	642, 1115, 1118, 1457
Tsp45I	GTSAC	1 cut(s)	683
TspDTI	ATGAA	1 cut(s)	1132
TspRI	CASTG	2 cut(s)	739, 1156
VpaK11BI	GGWCC	2 cut(s)	424, 1012
XagI	CCTNNNNNAGG	1 cut(s)	760
XapI	RAATTY	4 cut(s)	116, 863, 921, 934
XceI	RCATGY	4 cut(s)	461, 950, 966, 1240
XhoI	CTCGAG	1 cut(s)	1021
XmnI	GAANNNNTTC	1 cut(s)	1248
Zsp2I	ATGCAT	2 cut(s)	1059, 1483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.