Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Reverse (-)

65616927 .. 65621037

Gene Structure 4111 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG436900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGCGCGTTGCGTCCCACGCGCCACCCTGATATCCACCGCGTCATTTCCGAAACTGGTCGTCCCTGGCAGAACGAATCGCGTCGAGGCCTCGAAATCTGCGCTATTCGGCCGGAAATTCCACGCGCTGTGCCGTTACGATCGGAGAGACGCTGCGAGGCCATTGGTGAGGACGCGAGTCTTCGCTGAGCAGAAGGAGAAGGAGAAGGAGCTCCAGGTCAATGTGAGCGTTAGCATCGAACAAGGTTTGCCTGAGGATCACGAAATATCGAGGATTGCCGAATTGCTTAGGCTAAATGTTCGAACTGCATTGAACCTAGCGTTTGGTGGATTGAAAGATTCCGGTGGTGGTTTTGAAAGCGCGGAGCTTTCGGTGATTCTGTGCAATGATGAGTTTATGAGAAAACGTAACAAGGAATGGAGAGACGAGGACCGTGCTACTGGTGTTGTTTCCAAGTCCAAGCATGTGCCTGGTCTTAAGATTCCAAATGTTGTGTTGGGTGACATTGTGATTTCTGTTGAGATGGCGGCGAGGCAAGCAGAGCAAAGAGGCCACACACTTCTTGATGAGATGCGCCTTCTCATGGTGCAGGGATTGTTACATTTATTGAGATCTGATCATGAGATCAGTAAAGAGGCTACAGGGGAAATGGAGAAGGAGGAGGAACGTCTTTTAGACAGTCTTGGGTGGAAGGGGAAAAGAATGGTTGCATCTGATGCTGAAACCCATCCAAAAGCCAGCGAGGGGAAAATATGGAAGAAAGAAGGCAGCCTCCGACTCTATAAACCAAAGTTCAAATATGTGTTCTGTGACATGGATGGAACCCTCCTCAACAGCAAAAGTCAATTGAGTTCAACCACTGCCAGGGCTCTCAAAGAAGCCTCATCAAAGGGTGTGAAAATAGTGATTGCCACGGGTAAAGCTCGTCCAGCTGTGATGCGTGTCTTTAAGATGGTAGATTTAGCTGGAAAAGATGGCATTGTTTCAGAATTTTCTCCCGGGGTTTACTTGCAGGGATTGCTGGTTTATGGTGTTCAAGGTCAGGAAATTTGTAAAAGGAATTTAGATCCAAATGTCTGCAGAGAGGCATGTCTTTACTCATTGGAGAATAAGGTTCCTCTCATTGCATTCAGCAAGGACCGGTGCTCGAGTCTGTTTGATCACCCATATGTTGATGCTATGCATACTGTATATCATGAGCCAAAGGCAGAGATCATGCCTTCAGTTGAGCAGCTCTTGGCTGCTGCTGACATACAGAAACTGGTCTTCATGGACACTGCTGAGGGAGTATCTACTGCTCTGAGGCCGTATTGGTCAGAAGCAACTGGAGATCGTGCCACGGTTGTTCAAACCGTGCCGGACATGCTTGAACTTGTTCCCCCAGGAACCTCAAAAGGTAGTGGAGTAAAGATGTTGCTTGATCATTTGGGAATCACTCCAAAGGAGATCATGGCTATTGGCGATGGGGAGAATGATGTTGAGATGCTTGAGCTGGCTGGTTTGGGCATTTGTCTCAGTAATGGATCAGAGAAAACAAAAGCTGTGGCGAATGTAATCGGTGCCAGCAACGACGAAGATGGTGCTGCTGATGCAATCTACCGCTATGCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

537

Amino Acids

59.1

Weight (kDa)

7.16

Isoelectric Point (pI)

32.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	119 - 227	3.9e-23	Endoribonuclease YbeY
Hydrolase_3	PF08282	268 - 532	1.3e-50	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	438 - 500	1.1e-06	haloacid dehalogenase-like hydrolase
S6PP	PF05116	443 - 511	2.1e-08	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1559
AccII	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 362
AciI	CCGC	4 cut(s)	39, 362, 527, 1600
AclWI	GGATC	3 cut(s)	264, 1061, 1531
AcoI	YGGCCR	1 cut(s)	109
AcsI	RAATTY	4 cut(s)	116, 989, 1047, 1060
AcuI	CTGAAG	1 cut(s)	1206
AfiI	CCNNNNNNNGG	3 cut(s)	583, 744, 864
AflII	CTTAAG	1 cut(s)	476
AgeI	ACCGGT	1 cut(s)	1140
AgsI	TTSAA	8 cut(s)	313, 334, 356, 796, 855, 1037, 1349, 1370
AjnI	CCWGG	5 cut(s)	64, 213, 469, 863, 1381
AluBI	AGCT	8 cut(s)	211, 367, 923, 932, 965, 1234, 1492, 1541
AluI	AGCT	8 cut(s)	211, 367, 923, 932, 965, 1234, 1492, 1541
Alw21I	GWGCWC	2 cut(s)	213, 1148
Alw26I	GTCTC	3 cut(s)	141, 417, 1517
AlwI	GGATC	3 cut(s)	264, 1061, 1531
AlwNI	CAGNNNCTG	1 cut(s)	1261
Ama87I	CYCGRG	2 cut(s)	998, 1147
AoxI	GGCC	5 cut(s)	87, 109, 158, 550, 1304
ApeKI	GCWGC	6 cut(s)	152, 768, 1231, 1241, 1244, 1583
ApoI	RAATTY	4 cut(s)	116, 989, 1047, 1060
AsiGI	ACCGGT	1 cut(s)	1140
Asp700I	GAANNNNTTC	1 cut(s)	1374
AspLEI	GCGC	6 cut(s)	7, 23, 103, 127, 362, 576
AspS9I	GGNCC	2 cut(s)	430, 1138
AsuC2I	CCSGG	2 cut(s)	999, 1000
AsuHPI	GGTGA	4 cut(s)	178, 385, 512, 1154
AsuII	TTCGAA	1 cut(s)	301
AvaI	CYCGRG	2 cut(s)	998, 1147
AvaII	GGWCC	2 cut(s)	430, 1138
AxyI	CCTNAGG	1 cut(s)	252
BanI	GGYRCC	1 cut(s)	1559
BanII	GRGCYC	2 cut(s)	213, 871
BbsI	GAAGAC	2 cut(s)	172, 1258
Bbv12I	GWGCWC	2 cut(s)	213, 1148
BbvCI	CCTCAGC	1 cut(s)	1281
BbvI	GCAGC	6 cut(s)	139, 780, 1228, 1231, 1243, 1570
BccI	CCATC	7 cut(s)	517, 735, 812, 946, 968, 1457, 1571
BceAI	ACGGC	2 cut(s)	117, 1291
BcgI	CGANNNNNNTGC	4 cut(s)	416, 450, 1562, 1596
BciT130I	CCWGG	5 cut(s)	66, 215, 471, 865, 1383
BclI	TGATCA	3 cut(s)	616, 1159, 1420
BcnI	CCSGG	2 cut(s)	999, 1000
BcoDI	GTCTC	3 cut(s)	141, 417, 1517
BfaI	CTAG	1 cut(s)	317
BfmI	CTRYAG	2 cut(s)	639, 1078
BfrI	CTTAAG	1 cut(s)	476
BglII	AGATCT	1 cut(s)	611
BisI	GCNGC	7 cut(s)	153, 528, 769, 1232, 1242, 1245, 1584
BlpI	GCTNAGC	1 cut(s)	186
BlsI	GCNGC	7 cut(s)	154, 529, 770, 1233, 1243, 1246, 1585
Bme1390I	CCNGG	7 cut(s)	66, 215, 471, 865, 999, 1000, 1383
Bme18I	GGWCC	2 cut(s)	430, 1138
BmeT110I	CYCGRG	2 cut(s)	998, 1147
BmgT120I	GGNCC	2 cut(s)	430, 1138
BmiI	GGNNCC	4 cut(s)	823, 1116, 1387, 1561
BmrFI	CCNGG	7 cut(s)	66, 215, 471, 865, 999, 1000, 1383
BmsI	GCATC	8 cut(s)	243, 561, 706, 719, 927, 1165, 1473, 1579
BoxI	GACNNNNGTC	1 cut(s)	176
BpiI	GAAGAC	2 cut(s)	172, 1258
BpmI	CTGGAG	2 cut(s)	197, 1347
Bpu10I	CCTNAGC	2 cut(s)	287, 1281
Bpu1102I	GCTNAGC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	301
BpuEI	CTTGAG	1 cut(s)	1508
BpuMI	CCSGG	2 cut(s)	999, 1000
BsaJI	CCNNGG	7 cut(s)	64, 864, 912, 998, 999, 1338, 1381
BsaWI	WCCGGW	2 cut(s)	341, 1140
Bsc4I	CCNNNNNNNGG	3 cut(s)	583, 744, 864
Bse118I	RCCGGY	1 cut(s)	1140
Bse1I	ACTGG	4 cut(s)	60, 445, 1266, 1330
Bse21I	CCTNAGG	1 cut(s)	252
Bse3DI	GCAATG	2 cut(s)	391, 1122
BseBI	CCWGG	5 cut(s)	66, 215, 471, 865, 1383
BseDI	CCNNGG	7 cut(s)	64, 864, 912, 998, 999, 1338, 1381
BseGI	GGATG	2 cut(s)	727, 823
BseLI	CCNNNNNNNGG	3 cut(s)	583, 744, 864
BseMI	GCAATG	2 cut(s)	391, 1122
BseMII	CTCAG	5 cut(s)	177, 243, 1272, 1292, 1528
BseNI	ACTGG	4 cut(s)	60, 445, 1266, 1330
BseRI	GAGGAG	2 cut(s)	674, 818
BseX3I	CGGCCG	1 cut(s)	109
BseXI	GCAGC	6 cut(s)	139, 780, 1228, 1231, 1243, 1570
BsgI	GTGCAG	1 cut(s)	608
Bsh1236I	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 362
Bsh1285I	CGRYCG	2 cut(s)	112, 142
BshFI	GGCC	5 cut(s)	89, 111, 160, 552, 1306
BshNI	GGYRCC	1 cut(s)	1559
BshTI	ACCGGT	1 cut(s)	1140
BsiEI	CGRYCG	2 cut(s)	112, 142
BsiHKAI	GWGCWC	2 cut(s)	213, 1148
BsiHKCI	CYCGRG	2 cut(s)	998, 1147
BsiSI	CCGG	5 cut(s)	112, 342, 999, 1141, 1358
BslFI	GGGAC	1 cut(s)	47
BslI	CCNNNNNNNGG	3 cut(s)	583, 744, 864
BsmAI	GTCTC	3 cut(s)	141, 417, 1517
BsmBI	CGTCTC	2 cut(s)	141, 417
BsmFI	GGGAC	1 cut(s)	47
BsmI	GAATGC	1 cut(s)	1127
BsnI	GGCC	5 cut(s)	89, 111, 160, 552, 1306
BsoBI	CYCGRG	2 cut(s)	998, 1147
Bsp119I	TTCGAA	1 cut(s)	301
Bsp1286I	GDGCHC	3 cut(s)	213, 871, 1148
Bsp1720I	GCTNAGC	1 cut(s)	186
BspACI	CCGC	4 cut(s)	39, 362, 527, 1600
BspANI	GGCC	5 cut(s)	89, 111, 160, 552, 1306
BspCNI	CTCAG	5 cut(s)	178, 244, 1273, 1293, 1527
BspFNI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 362
BspHI	TCATGA	2 cut(s)	619, 1195
BspLI	GGNNCC	4 cut(s)	823, 1116, 1387, 1561
BspMAI	CTGCAG	1 cut(s)	1082
BspPI	GGATC	3 cut(s)	264, 1061, 1531
BspT104I	TTCGAA	1 cut(s)	301
BspT107I	GGYRCC	1 cut(s)	1559
BspTI	CTTAAG	1 cut(s)	476
BsrDI	GCAATG	2 cut(s)	391, 1122
BsrFI	RCCGGY	1 cut(s)	1140
BsrI	ACTGG	4 cut(s)	60, 445, 1266, 1330
BssAI	RCCGGY	1 cut(s)	1140
BssECI	CCNNGG	7 cut(s)	64, 864, 912, 998, 999, 1338, 1381
Bst2UI	CCWGG	5 cut(s)	66, 215, 471, 865, 1383
Bst4CI	ACNGT	5 cut(s)	434, 680, 1189, 1342, 1354
BstAFI	CTTAAG	1 cut(s)	476
BstAPI	GCANNNNNTGC	2 cut(s)	716, 1018
BstBI	TTCGAA	1 cut(s)	301
BstC8I	GCNNGC	4 cut(s)	537, 739, 1494, 1564
BstDEI	CTNAG	6 cut(s)	186, 252, 287, 1281, 1301, 1514
BstDSI	CCRYGG	2 cut(s)	912, 1338
BstF5I	GGATG	2 cut(s)	727, 823
BstFNI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 362
BstHHI	GCGC	6 cut(s)	7, 23, 103, 127, 362, 576
BstMAI	GTCTC	3 cut(s)	141, 417, 1517
BstMCI	CGRYCG	2 cut(s)	112, 142
BstMWI	GCNNNNNNNGC	8 cut(s)	18, 536, 541, 716, 929, 1018, 1363, 1589
BstNI	CCWGG	5 cut(s)	66, 215, 471, 865, 1383
BstNSI	RCATGY	3 cut(s)	467, 1092, 1366
BstPAI	GACNNNNGTC	1 cut(s)	176
BstSCI	CCNGG	7 cut(s)	64, 213, 469, 863, 997, 998, 1381
BstSFI	CTRYAG	2 cut(s)	639, 1078
BstUI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 362
BstV1I	GCAGC	6 cut(s)	139, 780, 1228, 1231, 1243, 1570
BstV2I	GAAGAC	2 cut(s)	172, 1258
BstX2I	RGATCY	2 cut(s)	611, 1066
BstYI	RGATCY	2 cut(s)	611, 1066
BstZI	CGGCCG	1 cut(s)	109
Bsu36I	CCTNAGG	1 cut(s)	252
BsuRI	GGCC	5 cut(s)	89, 111, 160, 552, 1306
BtgI	CCRYGG	2 cut(s)	912, 1338
BtgZI	GCGATG	1 cut(s)	1476
BtsCI	GGATG	2 cut(s)	727, 823
BtsI	GCAGTG	2 cut(s)	858, 1275
BtsIMutI	CAGTG	2 cut(s)	858, 1275
Cac8I	GCNNGC	4 cut(s)	537, 739, 1494, 1564
CaiI	CAGNNNCTG	1 cut(s)	1261
CciI	TCATGA	2 cut(s)	619, 1195
CfoI	GCGC	6 cut(s)	7, 23, 103, 127, 362, 576
Cfr10I	RCCGGY	1 cut(s)	1140
Cfr13I	GGNCC	2 cut(s)	430, 1138
Cfr9I	CCCGGG	1 cut(s)	998
CseI	GACGC	4 cut(s)	30, 70, 158, 181
CspAI	ACCGGT	1 cut(s)	1140
CspCI	CAANNNNNGTGG	2 cut(s)	1524, 1559
DdeI	CTNAG	6 cut(s)	186, 252, 287, 1281, 1301, 1514
EaeI	YGGCCR	1 cut(s)	109
EagI	CGGCCG	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	211
EclXI	CGGCCG	1 cut(s)	109
Eco147I	AGGCCT	1 cut(s)	89
Eco24I	GRGCYC	2 cut(s)	213, 871
Eco32I	GATATC	1 cut(s)	33
Eco47I	GGWCC	2 cut(s)	430, 1138
Eco52I	CGGCCG	1 cut(s)	109
Eco53kI	GAGCTC	1 cut(s)	211
Eco57I	CTGAAG	1 cut(s)	1206
Eco81I	CCTNAGG	1 cut(s)	252
Eco88I	CYCGRG	2 cut(s)	998, 1147
EcoICRI	GAGCTC	1 cut(s)	211
EcoRII	CCWGG	5 cut(s)	64, 213, 469, 863, 1381
EcoRV	GATATC	1 cut(s)	33
EcoT22I	ATGCAT	2 cut(s)	1185, 1609
EcoT38I	GRGCYC	2 cut(s)	213, 871
Esp3I	CGTCTC	2 cut(s)	141, 417
FaqI	GGGAC	1 cut(s)	47
FauNDI	CATATG	1 cut(s)	1168
FbaI	TGATCA	3 cut(s)	616, 1159, 1420
Fnu4HI	GCNGC	7 cut(s)	153, 528, 769, 1232, 1242, 1245, 1584
FokI	GGATG	2 cut(s)	714, 830
FriOI	GRGCYC	2 cut(s)	213, 871
Fsp4HI	GCNGC	7 cut(s)	153, 528, 769, 1232, 1242, 1245, 1584
FspBI	CTAG	1 cut(s)	317
GlaI	GCGC	6 cut(s)	6, 22, 102, 126, 361, 575
GluI	GCNGC	7 cut(s)	153, 528, 769, 1232, 1242, 1245, 1584
GsuI	CTGGAG	2 cut(s)	197, 1347
HaeIII	GGCC	5 cut(s)	89, 111, 160, 552, 1306
HapII	CCGG	5 cut(s)	112, 342, 999, 1141, 1358
HgaI	GACGC	4 cut(s)	30, 70, 158, 181
HhaI	GCGC	6 cut(s)	7, 23, 103, 127, 362, 576
Hin6I	GCGC	6 cut(s)	5, 21, 101, 125, 360, 574
HinP1I	GCGC	6 cut(s)	5, 21, 101, 125, 360, 574
HinfI	GANTC	8 cut(s)	76, 177, 338, 376, 481, 777, 1150, 1431
HpaII	CCGG	5 cut(s)	112, 342, 999, 1141, 1358
HphI	GGTGA	4 cut(s)	178, 385, 512, 1154
Hpy166II	GTNNAC	1 cut(s)	1006
Hpy188I	TCNGA	9 cut(s)	51, 144, 616, 715, 776, 988, 1302, 1318, 1528
Hpy188III	TCNNGA	5 cut(s)	260, 563, 620, 1043, 1196
Hpy8I	GTNNAC	1 cut(s)	1006
Hpy99I	CGWCG	2 cut(s)	86, 1574
HpyAV	CCTTC	8 cut(s)	187, 193, 199, 587, 649, 685, 758, 1230
HpyCH4III	ACNGT	5 cut(s)	434, 680, 1189, 1342, 1354
HpyCH4IV	ACGT	2 cut(s)	406, 667
HpyF10VI	GCNNNNNNNGC	8 cut(s)	18, 536, 541, 716, 929, 1018, 1363, 1589
HpyF3I	CTNAG	6 cut(s)	186, 252, 287, 1281, 1301, 1514
HpySE526I	ACGT	2 cut(s)	406, 667
HspAI	GCGC	6 cut(s)	5, 21, 101, 125, 360, 574
Ksp22I	TGATCA	3 cut(s)	616, 1159, 1420
LmnI	GCTCC	3 cut(s)	208, 216, 364
Lsp1109I	GCAGC	6 cut(s)	139, 780, 1228, 1231, 1243, 1570
LweI	GCATC	8 cut(s)	243, 561, 706, 719, 927, 1165, 1473, 1579
MaeI	CTAG	1 cut(s)	317
MaeII	ACGT	2 cut(s)	406, 667
MaeIII	GTNAC	5 cut(s)	134, 407, 500, 597, 809
MboII	GAAGA	4 cut(s)	172, 769, 1258, 1586
MfeI	CAATTG	1 cut(s)	845
MflI	RGATCY	2 cut(s)	611, 1066
MhlI	GDGCHC	3 cut(s)	213, 871, 1148
MluCI	AATT	6 cut(s)	116, 281, 845, 989, 1047, 1060
MlyI	GAGTC	3 cut(s)	186, 771, 1159
MmeI	TCCRAC	1 cut(s)	799
Mph1103I	ATGCAT	2 cut(s)	1185, 1609
MroXI	GAANNNNTTC	1 cut(s)	1374
MseI	TTAA	2 cut(s)	477, 948
MslI	CAYNNNNRTG	1 cut(s)	1167
MspA1I	CMGCKG	1 cut(s)	932
MspCI	CTTAAG	1 cut(s)	476
MspI	CCGG	5 cut(s)	112, 342, 999, 1141, 1358
MspR9I	CCNGG	7 cut(s)	66, 215, 471, 865, 999, 1000, 1383
MunI	CAATTG	1 cut(s)	845
Mva1269I	GAATGC	1 cut(s)	1127
MvaI	CCWGG	5 cut(s)	66, 215, 471, 865, 1383
MvnI	CGCG	7 cut(s)	7, 21, 41, 81, 125, 175, 362
MwoI	GCNNNNNNNGC	8 cut(s)	18, 536, 541, 716, 929, 1018, 1363, 1589
NciI	CCSGG	2 cut(s)	999, 1000
NdeI	CATATG	1 cut(s)	1168
NlaIV	GGNNCC	4 cut(s)	823, 1116, 1387, 1561
NmuCI	GTSAC	2 cut(s)	500, 809
NsiI	ATGCAT	2 cut(s)	1185, 1609
NspI	RCATGY	3 cut(s)	467, 1092, 1366
NspV	TTCGAA	1 cut(s)	301
PaeR7I	CTCGAG	1 cut(s)	1147
PagI	TCATGA	2 cut(s)	619, 1195
PceI	AGGCCT	1 cut(s)	89
PctI	GAATGC	1 cut(s)	1127
PdmI	GAANNNNTTC	1 cut(s)	1374
PfeI	GAWTC	5 cut(s)	76, 338, 376, 481, 1431
PinAI	ACCGGT	1 cut(s)	1140
PkrI	GCNGC	7 cut(s)	154, 529, 770, 1233, 1243, 1246, 1585
Ple19I	CGATCG	1 cut(s)	142
PleI	GAGTC	3 cut(s)	185, 771, 1158
PpsI	GAGTC	3 cut(s)	185, 771, 1158
PshAI	GACNNNNGTC	1 cut(s)	176
Psp124BI	GAGCTC	1 cut(s)	213
Psp6I	CCWGG	5 cut(s)	64, 213, 469, 863, 1381
PspGI	CCWGG	5 cut(s)	64, 213, 469, 863, 1381
PspN4I	GGNNCC	4 cut(s)	823, 1116, 1387, 1561
PspPI	GGNCC	2 cut(s)	430, 1138
PspXI	VCTCGAGB	1 cut(s)	1147
PstI	CTGCAG	1 cut(s)	1082
PstNI	CAGNNNCTG	1 cut(s)	1261
PsuI	RGATCY	2 cut(s)	611, 1066
PvuI	CGATCG	1 cut(s)	142
PvuII	CAGCTG	1 cut(s)	932
RseI	CAYNNNNRTG	1 cut(s)	1167
SacI	GAGCTC	1 cut(s)	213
SaqAI	TTAA	2 cut(s)	477, 948
SatI	GCNGC	7 cut(s)	153, 528, 769, 1232, 1242, 1245, 1584
Sau96I	GGNCC	2 cut(s)	430, 1138
SchI	GAGTC	3 cut(s)	186, 771, 1159
ScrFI	CCNGG	7 cut(s)	66, 215, 471, 865, 999, 1000, 1383
SduI	GDGCHC	3 cut(s)	213, 871, 1148
SfaNI	GCATC	8 cut(s)	243, 561, 706, 719, 927, 1165, 1473, 1579
SfcI	CTRYAG	2 cut(s)	639, 1078
Sfr274I	CTCGAG	1 cut(s)	1147
SfuI	TTCGAA	1 cut(s)	301
SinI	GGWCC	2 cut(s)	430, 1138
SlaI	CTCGAG	1 cut(s)	1147
SmaI	CCCGGG	1 cut(s)	1000
SmiMI	CAYNNNNRTG	1 cut(s)	1167
SmlI	CTYRAG	3 cut(s)	476, 1147, 1487
SmoI	CTYRAG	3 cut(s)	476, 1147, 1487
Sse9I	AATT	6 cut(s)	116, 281, 845, 989, 1047, 1060
SseBI	AGGCCT	1 cut(s)	89
SsiI	CCGC	4 cut(s)	39, 362, 527, 1600
SspMI	CTAG	1 cut(s)	317
SstI	GAGCTC	1 cut(s)	213
StuI	AGGCCT	1 cut(s)	89
StyD4I	CCNGG	7 cut(s)	64, 213, 469, 863, 997, 998, 1381
TaaI	ACNGT	5 cut(s)	434, 680, 1189, 1342, 1354
TaiI	ACGT	2 cut(s)	409, 670
TaqI	TCGA	6 cut(s)	84, 92, 237, 269, 301, 1148
TasI	AATT	6 cut(s)	116, 281, 845, 989, 1047, 1060
TauI	GCSGC	1 cut(s)	530
TfiI	GAWTC	5 cut(s)	76, 338, 376, 481, 1431
Tru1I	TTAA	2 cut(s)	477, 948
Tru9I	TTAA	2 cut(s)	477, 948
TscAI	CASTG	2 cut(s)	865, 1282
TseFI	GTSAC	2 cut(s)	500, 809
TseI	GCWGC	6 cut(s)	152, 768, 1231, 1241, 1244, 1583
Tsp45I	GTSAC	2 cut(s)	500, 809
TspDTI	ATGAA	1 cut(s)	1258
TspMI	CCCGGG	1 cut(s)	998
TspRI	CASTG	2 cut(s)	865, 1282
Vha464I	CTTAAG	1 cut(s)	476
VpaK11BI	GGWCC	2 cut(s)	430, 1138
XapI	RAATTY	4 cut(s)	116, 989, 1047, 1060
XceI	RCATGY	3 cut(s)	467, 1092, 1366
XhoI	CTCGAG	1 cut(s)	1147
XmaI	CCCGGG	1 cut(s)	998
XmnI	GAANNNNTTC	1 cut(s)	1374
XspI	CTAG	1 cut(s)	317
Zsp2I	ATGCAT	2 cut(s)	1185, 1609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.