Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0003247

Physical Location & Seq

Genomic Coordinates Forward (+)

64238858 .. 64243366

Gene Structure 4509 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G040430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1851 bp

ATGCCCTCGGCTTTCTATATTCTATGCGAAATTACAAGAAACTCCCCGCTGGTTTTTTTGACTTGTTTGACTTTCATTTTAACCGGTAACGTCGCCGTTACTATCAGTTTGTCACTCCCGAAGCGCGAACAAACCAAACCAACCTTCTGTCTAGTCTCCTCCGCCACTCAAATGGCGCGTTGCGTCCCACGCGCCTCCCTGATATCCACCGCGTCATTTCCGAAACTGGTCGTCCCTGGCAGAACGAATCGCTTCGAGGCCTCGAAATCTGCGCTATTCGGCCGGAAATTCCACGTTCTGTGCCGTTACGATCGGAGAGACGCTGCGAGGCCATTGGTGAGGACGCGAGTCTTCGCTGAGCAGAAGGAGAAGGAGAAGGAGCTCCAGCTCAATGTGAGCGTTAGCATCGAACAAGGTTTGCCTGAGGATCACGAAATATCGAGGATTGCCGAATTGCTTAGGCTAAATGTTCGAACTGCATTGAACCTAGCGTTTGGTGGATTGAAAGATTCTGGTGATGGTTTTGAAAGCGCGGAGCTTTCGGTGATTGTGTGCAATGATGTATTTATGAGAAAACGTAACAAGGAATGGAGAGACGAGGACCGTGCTACTGGTGTTGTTTCCAAGTCCAAGCATGTGTCTGGTCTTAAGATTCCAAATGTTGTGTTGGGTGACATTGTGATTTCTGTTGAGATGGCGGCGAGGCAAGCAGAGCAAAGAGGCCACACACTTCTTGATGAGATGCGCCTTCTCATGGTGCAGGGATTGTTACATTTATTGAGGTTTGATCATGAGATCAGTAAAGAGGCTACAGGGGAAATGGAGAAGGAGGAGGAACGTCTTTTAGACAGTCTTGGGTGGAAGGGGAAAAGAATGGTTGCAGTTTCAGTTTCAGTTTCCGAAACCCATCCAAAATCCAACGAGGGGAAAAATGGAGAAGGAGGAGGAACGTCTTTTAGACAGTCTTGGGTGGAAGGGGAAAAGAATGACAGGAAGAAAGAAGGCAGCCTCCGATTTTATAAACCAAAGTTCAAATATGTGTTCTGTGACATGGATGGAACCCTCCTCAACAGCAAAAGTCAATTGAGTTCAACCACTGCCAGGGCTCTCAAAGAAGCCTCATCAAGGGGTGTGAAAATAGTGATTGCCACCGGTAAAGCTCGTCCAGCTGTGATGCGCGTCTTTAAGATGGTAGATTTAGCTGGAAAAGATGGCATTGTTTCAGAATTTTCTCCTGGGGTTTACTTGCAGGGATTGCTGGTTTATGGTATTCAAGGTCAGGAAATTTGTAAAAAGAATTTAGATCCAAATGTCTGCAGAGAGGCATGTCTTTACTCATTGGAGAATAAGGTTCCTCTCATTGCATTCAGCAAGGACCGGTGCTCGAGTCTGTTTGATCACCCATATGTTGATGCTATGCATACTGTATATCATGAGCCAAAGGCAGAAATCATGCCTTCAGTTGAGCAGTTCTTGGCTGCTGCTGACATACAGAAACTGGTCTTCATGGACACTGCTGAGGGAGTATCTACTGCTTTGAGGCCGTATTGGTCAGAAGCAACTGGAGATCGTGCCACAGTTGTTCAAACTGTGCCGGACATGCTTGAACTTGTTCCCCCAGGAACCTCAAAAGGCAGTGGAGTAAAGATGTTGCTTGATCATTTGGGAATCACTCCAAAGGAGATCATGGCTATTGGCGATGGGGAAAATGATGTTGAGATGCTTGAGCTGGCTGGTTTGGGCATTTGTCTCAGTAATGGAGCAGAAAAAACAAAAGCTGTGGCCAATGTAATCGGTGCCAGCAACGACGAAGATGGTGCGGCTGATGCAATCTACCGCTATGCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901259 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_3 UPF0054

Protein Analysis

616

Amino Acids

67.91

Weight (kDa)

7.88

Isoelectric Point (pI)

33.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
YbeY	PF02130	176 - 284	2.1e-23	Endoribonuclease YbeY
Hydrolase_3	PF08282	347 - 611	1.2e-49	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	519 - 579	1.6e-06	haloacid dehalogenase-like hydrolase
S6PP	PF05116	522 - 590	3.8e-08	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003247)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G25870
fragaria_vesca	FvH4_2g35220 FvH4_2g35230
malus_domestica	MD08G1110200.v1.1 MD08G1110300.v1.1
prunus_persica	Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444400_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1 Prupe.1G444500_v2.0.a1
pyrus_communis	pycom08g09180 pycom15g08410
rosa_chinensis	RchiOBHm_Chr6g0306701 RchiOBHm_Chr6g0306711
rosa_laevigata	RLG00000010771 RLG00000010772
rosa_multiflora	Rmu_sc0005201.1_g000001 Rmu_sc0017725.1_g000001 Rmu_sc0017785.1_g000007 Rmu_sc0017785.1_g000008 Rmu_sc0017786.1_g000007
rosa_roxburghii	Rroxscaffold_7G00161520 Rroxscaffold_7G00161530
rosa_rugosa	Rorug06G0351700 Rorug06G0351700
rosa_samantha	Rh6AG464100 Rh6AG464200 Rh6BG436800 Rh6BG436900 Rh6CG478300 Rh6DG465000 Rh6DG465100
rosa_wichuraiana	Rw6G040430 Rw6G040440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1020
AccB1I	GGYRCC	1 cut(s)	1796
AccII	CGCG	7 cut(s)	126, 178, 192, 212, 346, 533, 1179
AciI	CCGC	7 cut(s)	47, 162, 210, 533, 698, 1820, 1837
AclWI	GGATC	2 cut(s)	435, 1298
AcoI	YGGCCR	2 cut(s)	280, 1782
AcsI	RAATTY	4 cut(s)	287, 1226, 1284, 1297
AcuI	CTGAAG	1 cut(s)	1443
AfiI	CCNNNNNNNGG	4 cut(s)	754, 924, 1101, 1125
AflII	CTTAAG	1 cut(s)	647
AgeI	ACCGGT	3 cut(s)	83, 1151, 1377
AgsI	TTSAA	8 cut(s)	484, 505, 527, 1033, 1092, 1274, 1586, 1607
AjnI	CCWGG	4 cut(s)	235, 1100, 1234, 1618
AjuI	GAANNNNNNNTTGG	2 cut(s)	128, 160
AluBI	AGCT	8 cut(s)	382, 388, 538, 1160, 1169, 1202, 1729, 1778
AluI	AGCT	8 cut(s)	382, 388, 538, 1160, 1169, 1202, 1729, 1778
Alw21I	GWGCWC	2 cut(s)	384, 1385
Alw26I	GTCTC	4 cut(s)	160, 312, 588, 1754
AlwI	GGATC	2 cut(s)	435, 1298
AlwNI	CAGNNNCTG	1 cut(s)	1498
Ama87I	CYCGRG	1 cut(s)	1384
AoxI	GGCC	6 cut(s)	258, 280, 329, 721, 1541, 1782
ApeKI	GCWGC	4 cut(s)	323, 1005, 1478, 1481
ApoI	RAATTY	4 cut(s)	287, 1226, 1284, 1297
AsiGI	ACCGGT	3 cut(s)	83, 1151, 1377
Asp700I	GAANNNNTTC	2 cut(s)	251, 1611
AspLEI	GCGC	7 cut(s)	126, 178, 194, 274, 533, 747, 1179
AspS9I	GGNCC	2 cut(s)	601, 1375
AsuHPI	GGTGA	5 cut(s)	349, 527, 556, 683, 1391
AsuII	TTCGAA	1 cut(s)	472
AvaI	CYCGRG	1 cut(s)	1384
AvaII	GGWCC	2 cut(s)	601, 1375
AxyI	CCTNAGG	1 cut(s)	423
BalI	TGGCCA	1 cut(s)	1784
BanI	GGYRCC	1 cut(s)	1796
BanII	GRGCYC	2 cut(s)	384, 1108
BbsI	GAAGAC	2 cut(s)	343, 1495
Bbv12I	GWGCWC	2 cut(s)	384, 1385
BbvCI	CCTCAGC	1 cut(s)	1518
BbvI	GCAGC	4 cut(s)	310, 1017, 1465, 1468
BccI	CCATC	8 cut(s)	512, 688, 915, 1049, 1183, 1205, 1694, 1808
BceAI	ACGGC	3 cut(s)	80, 288, 1528
BcgI	CGANNNNNNTGC	4 cut(s)	587, 621, 1799, 1833
BciT130I	CCWGG	4 cut(s)	237, 1102, 1236, 1620
BclI	TGATCA	3 cut(s)	787, 1396, 1657
BcoDI	GTCTC	4 cut(s)	160, 312, 588, 1754
BfaI	CTAG	2 cut(s)	152, 488
BfmI	CTRYAG	2 cut(s)	810, 1315
BfrI	CTTAAG	1 cut(s)	647
BisI	GCNGC	6 cut(s)	324, 699, 1006, 1479, 1482, 1821
BlpI	GCTNAGC	1 cut(s)	357
BlsI	GCNGC	6 cut(s)	325, 700, 1007, 1480, 1483, 1822
Bme1390I	CCNGG	4 cut(s)	237, 1102, 1236, 1620
Bme18I	GGWCC	2 cut(s)	601, 1375
BmeT110I	CYCGRG	1 cut(s)	1384
BmgT120I	GGNCC	2 cut(s)	601, 1375
BmiI	GGNNCC	4 cut(s)	1060, 1353, 1624, 1798
BmrFI	CCNGG	4 cut(s)	237, 1102, 1236, 1620
BmsI	GCATC	6 cut(s)	414, 732, 1164, 1402, 1710, 1816
BoxI	GACNNNNGTC	1 cut(s)	347
BpiI	GAAGAC	2 cut(s)	343, 1495
BpmI	CTGGAG	2 cut(s)	368, 1584
Bpu10I	CCTNAGC	2 cut(s)	458, 1518
Bpu1102I	GCTNAGC	1 cut(s)	357
Bpu14I	TTCGAA	1 cut(s)	472
BpuEI	CTTGAG	1 cut(s)	1745
BsaJI	CCNNGG	5 cut(s)	6, 235, 1101, 1235, 1618
BsaWI	WCCGGW	3 cut(s)	83, 1151, 1377
Bsc4I	CCNNNNNNNGG	4 cut(s)	754, 924, 1101, 1125
Bse118I	RCCGGY	3 cut(s)	83, 1151, 1377
Bse1I	ACTGG	4 cut(s)	231, 616, 1503, 1567
Bse21I	CCTNAGG	1 cut(s)	423
Bse3DI	GCAATG	2 cut(s)	562, 1359
BseBI	CCWGG	4 cut(s)	237, 1102, 1236, 1620
BseDI	CCNNGG	5 cut(s)	6, 235, 1101, 1235, 1618
BseGI	GGATG	2 cut(s)	907, 1060
BseLI	CCNNNNNNNGG	4 cut(s)	754, 924, 1101, 1125
BseMI	GCAATG	2 cut(s)	562, 1359
BseMII	CTCAG	4 cut(s)	348, 414, 1509, 1765
BseNI	ACTGG	4 cut(s)	231, 616, 1503, 1567
BseRI	GAGGAG	4 cut(s)	148, 845, 957, 1055
BseX3I	CGGCCG	1 cut(s)	280
BseXI	GCAGC	4 cut(s)	310, 1017, 1465, 1468
BsgI	GTGCAG	1 cut(s)	779
Bsh1236I	CGCG	7 cut(s)	126, 178, 192, 212, 346, 533, 1179
Bsh1285I	CGRYCG	2 cut(s)	283, 313
BshFI	GGCC	6 cut(s)	260, 282, 331, 723, 1543, 1784
BshNI	GGYRCC	1 cut(s)	1796
BshTI	ACCGGT	3 cut(s)	83, 1151, 1377
BsiEI	CGRYCG	2 cut(s)	283, 313
BsiHKAI	GWGCWC	2 cut(s)	384, 1385
BsiHKCI	CYCGRG	1 cut(s)	1384
BsiSI	CCGG	5 cut(s)	84, 283, 1152, 1378, 1595
BslFI	GGGAC	2 cut(s)	170, 218
BslI	CCNNNNNNNGG	4 cut(s)	754, 924, 1101, 1125
BsmAI	GTCTC	4 cut(s)	160, 312, 588, 1754
BsmBI	CGTCTC	2 cut(s)	312, 588
BsmFI	GGGAC	2 cut(s)	170, 218
BsmI	GAATGC	1 cut(s)	1364
BsnI	GGCC	6 cut(s)	260, 282, 331, 723, 1543, 1784
BsoBI	CYCGRG	1 cut(s)	1384
Bsp119I	TTCGAA	1 cut(s)	472
Bsp1286I	GDGCHC	3 cut(s)	384, 1108, 1385
Bsp143I	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
Bsp1720I	GCTNAGC	1 cut(s)	357
BspACI	CCGC	7 cut(s)	47, 162, 210, 533, 698, 1820, 1837
BspANI	GGCC	6 cut(s)	260, 282, 331, 723, 1543, 1784
BspCNI	CTCAG	4 cut(s)	349, 415, 1510, 1764
BspFNI	CGCG	7 cut(s)	126, 178, 192, 212, 346, 533, 1179
BspHI	TCATGA	2 cut(s)	790, 1432
BspLI	GGNNCC	4 cut(s)	1060, 1353, 1624, 1798
BspMAI	CTGCAG	1 cut(s)	1319
BspPI	GGATC	2 cut(s)	435, 1298
BspT104I	TTCGAA	1 cut(s)	472
BspT107I	GGYRCC	1 cut(s)	1796
BspTI	CTTAAG	1 cut(s)	647
BsrDI	GCAATG	2 cut(s)	562, 1359
BsrFI	RCCGGY	3 cut(s)	83, 1151, 1377
BsrI	ACTGG	4 cut(s)	231, 616, 1503, 1567
BssAI	RCCGGY	3 cut(s)	83, 1151, 1377
BssECI	CCNNGG	5 cut(s)	6, 235, 1101, 1235, 1618
BssMI	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
Bst2UI	CCWGG	4 cut(s)	237, 1102, 1236, 1620
Bst4CI	ACNGT	6 cut(s)	605, 851, 963, 1426, 1579, 1591
BstAFI	CTTAAG	1 cut(s)	647
BstAPI	GCANNNNNTGC	1 cut(s)	1255
BstBI	TTCGAA	1 cut(s)	472
BstC8I	GCNNGC	3 cut(s)	708, 1731, 1801
BstDEI	CTNAG	5 cut(s)	357, 423, 458, 1518, 1751
BstENI	CCTNNNNNAGG	1 cut(s)	1123
BstF5I	GGATG	2 cut(s)	907, 1060
BstFNI	CGCG	7 cut(s)	126, 178, 192, 212, 346, 533, 1179
BstHHI	GCGC	7 cut(s)	126, 178, 194, 274, 533, 747, 1179
BstKTI	GATC	9 cut(s)	313, 430, 790, 798, 1306, 1399, 1570, 1660, 1686
BstMAI	GTCTC	4 cut(s)	160, 312, 588, 1754
BstMBI	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
BstMCI	CGRYCG	2 cut(s)	283, 313
BstMWI	GCNNNNNNNGC	7 cut(s)	189, 707, 712, 1166, 1255, 1600, 1826
BstNI	CCWGG	4 cut(s)	237, 1102, 1236, 1620
BstNSI	RCATGY	3 cut(s)	638, 1329, 1603
BstPAI	GACNNNNGTC	1 cut(s)	347
BstSCI	CCNGG	4 cut(s)	235, 1100, 1234, 1618
BstSFI	CTRYAG	2 cut(s)	810, 1315
BstUI	CGCG	7 cut(s)	126, 178, 192, 212, 346, 533, 1179
BstV1I	GCAGC	4 cut(s)	310, 1017, 1465, 1468
BstV2I	GAAGAC	2 cut(s)	343, 1495
BstX2I	RGATCY	1 cut(s)	1303
BstXI	CCANNNNNNTGG	1 cut(s)	172
BstYI	RGATCY	1 cut(s)	1303
BstZI	CGGCCG	1 cut(s)	280
Bsu36I	CCTNAGG	1 cut(s)	423
BsuRI	GGCC	6 cut(s)	260, 282, 331, 723, 1543, 1784
BtgZI	GCGATG	1 cut(s)	1713
BtsCI	GGATG	2 cut(s)	907, 1060
BtsI	GCAGTG	3 cut(s)	1095, 1512, 1642
BtsIMutI	CAGTG	3 cut(s)	1095, 1512, 1642
Cac8I	GCNNGC	3 cut(s)	708, 1731, 1801
CaiI	CAGNNNCTG	1 cut(s)	1498
CciI	TCATGA	2 cut(s)	790, 1432
CfoI	GCGC	7 cut(s)	126, 178, 194, 274, 533, 747, 1179
Cfr10I	RCCGGY	3 cut(s)	83, 1151, 1377
Cfr13I	GGNCC	2 cut(s)	601, 1375
CseI	GACGC	5 cut(s)	172, 201, 329, 352, 1168
CspAI	ACCGGT	3 cut(s)	83, 1151, 1377
CspCI	CAANNNNNGTGG	2 cut(s)	1761, 1796
DdeI	CTNAG	5 cut(s)	357, 423, 458, 1518, 1751
DpnI	GATC	9 cut(s)	312, 429, 789, 797, 1305, 1398, 1569, 1659, 1685
DpnII	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
EaeI	YGGCCR	2 cut(s)	280, 1782
EagI	CGGCCG	1 cut(s)	280
EciI	GGCGGA	1 cut(s)	151
Ecl136II	GAGCTC	1 cut(s)	382
EclXI	CGGCCG	1 cut(s)	280
Eco147I	AGGCCT	1 cut(s)	260
Eco24I	GRGCYC	2 cut(s)	384, 1108
Eco32I	GATATC	1 cut(s)	204
Eco47I	GGWCC	2 cut(s)	601, 1375
Eco52I	CGGCCG	1 cut(s)	280
Eco53kI	GAGCTC	1 cut(s)	382
Eco57I	CTGAAG	1 cut(s)	1443
Eco81I	CCTNAGG	1 cut(s)	423
Eco88I	CYCGRG	1 cut(s)	1384
EcoICRI	GAGCTC	1 cut(s)	382
EcoNI	CCTNNNNNAGG	1 cut(s)	1123
EcoRII	CCWGG	4 cut(s)	235, 1100, 1234, 1618
EcoRV	GATATC	1 cut(s)	204
EcoT22I	ATGCAT	1 cut(s)	1422
EcoT38I	GRGCYC	2 cut(s)	384, 1108
Esp3I	CGTCTC	2 cut(s)	312, 588
FaqI	GGGAC	2 cut(s)	170, 218
FauI	CCCGC	1 cut(s)	54
FauNDI	CATATG	1 cut(s)	1405
FbaI	TGATCA	3 cut(s)	787, 1396, 1657
Fnu4HI	GCNGC	6 cut(s)	324, 699, 1006, 1479, 1482, 1821
FokI	GGATG	2 cut(s)	894, 1067
FriOI	GRGCYC	2 cut(s)	384, 1108
Fsp4HI	GCNGC	6 cut(s)	324, 699, 1006, 1479, 1482, 1821
FspBI	CTAG	2 cut(s)	152, 488
GlaI	GCGC	7 cut(s)	125, 177, 193, 273, 532, 746, 1178
GluI	GCNGC	6 cut(s)	324, 699, 1006, 1479, 1482, 1821
GsuI	CTGGAG	2 cut(s)	368, 1584
HaeIII	GGCC	6 cut(s)	260, 282, 331, 723, 1543, 1784
HapII	CCGG	5 cut(s)	84, 283, 1152, 1378, 1595
HgaI	GACGC	5 cut(s)	172, 201, 329, 352, 1168
HhaI	GCGC	7 cut(s)	126, 178, 194, 274, 533, 747, 1179
Hin6I	GCGC	7 cut(s)	124, 176, 192, 272, 531, 745, 1177
HinP1I	GCGC	7 cut(s)	124, 176, 192, 272, 531, 745, 1177
HinfI	GANTC	6 cut(s)	247, 348, 509, 652, 1387, 1668
HpaII	CCGG	5 cut(s)	84, 283, 1152, 1378, 1595
HphI	GGTGA	5 cut(s)	349, 527, 556, 683, 1391
Hpy166II	GTNNAC	1 cut(s)	1243
Hpy188I	TCNGA	6 cut(s)	222, 315, 901, 1013, 1225, 1555
Hpy188III	TCNNGA	6 cut(s)	118, 431, 734, 791, 1280, 1433
Hpy8I	GTNNAC	1 cut(s)	1243
Hpy99I	CGWCG	2 cut(s)	95, 1811
HpyCH4III	ACNGT	6 cut(s)	605, 851, 963, 1426, 1579, 1591
HpyCH4IV	ACGT	5 cut(s)	90, 294, 577, 838, 950
HpyCH4V	TGCA	9 cut(s)	479, 555, 760, 881, 1249, 1317, 1364, 1420, 1829
HpyF10VI	GCNNNNNNNGC	7 cut(s)	189, 707, 712, 1166, 1255, 1600, 1826
HpyF3I	CTNAG	5 cut(s)	357, 423, 458, 1518, 1751
HpySE526I	ACGT	5 cut(s)	90, 294, 577, 838, 950
HspAI	GCGC	7 cut(s)	124, 176, 192, 272, 531, 745, 1177
Ksp22I	TGATCA	3 cut(s)	787, 1396, 1657
Kzo9I	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
LmnI	GCTCC	4 cut(s)	379, 387, 535, 1760
Lsp1109I	GCAGC	4 cut(s)	310, 1017, 1465, 1468
LweI	GCATC	6 cut(s)	414, 732, 1164, 1402, 1710, 1816
MaeI	CTAG	2 cut(s)	152, 488
MaeII	ACGT	5 cut(s)	90, 294, 577, 838, 950
MaeIII	GTNAC	8 cut(s)	86, 97, 111, 305, 578, 671, 768, 1046
MalI	GATC	9 cut(s)	312, 429, 789, 797, 1305, 1398, 1569, 1659, 1685
MboI	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
MboII	GAAGA	4 cut(s)	343, 1006, 1495, 1823
MfeI	CAATTG	1 cut(s)	1082
MflI	RGATCY	1 cut(s)	1303
MhlI	GDGCHC	3 cut(s)	384, 1108, 1385
MlsI	TGGCCA	1 cut(s)	1784
MluCI	AATT	7 cut(s)	30, 287, 452, 1082, 1226, 1284, 1297
MluNI	TGGCCA	1 cut(s)	1784
MlyI	GAGTC	2 cut(s)	357, 1396
MmeI	TCCRAC	1 cut(s)	942
Mox20I	TGGCCA	1 cut(s)	1784
Mph1103I	ATGCAT	1 cut(s)	1422
MroXI	GAANNNNTTC	2 cut(s)	251, 1611
MscI	TGGCCA	1 cut(s)	1784
MseI	TTAA	3 cut(s)	80, 648, 1185
MslI	CAYNNNNRTG	2 cut(s)	170, 1404
Msp20I	TGGCCA	1 cut(s)	1784
MspA1I	CMGCKG	2 cut(s)	49, 1169
MspCI	CTTAAG	1 cut(s)	647
MspI	CCGG	5 cut(s)	84, 283, 1152, 1378, 1595
MspR9I	CCNGG	4 cut(s)	237, 1102, 1236, 1620
MunI	CAATTG	1 cut(s)	1082
Mva1269I	GAATGC	1 cut(s)	1364
MvaI	CCWGG	4 cut(s)	237, 1102, 1236, 1620
MvnI	CGCG	7 cut(s)	126, 178, 192, 212, 346, 533, 1179
MwoI	GCNNNNNNNGC	7 cut(s)	189, 707, 712, 1166, 1255, 1600, 1826
NdeI	CATATG	1 cut(s)	1405
NdeII	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
NlaIV	GGNNCC	4 cut(s)	1060, 1353, 1624, 1798
NmuCI	GTSAC	3 cut(s)	111, 671, 1046
NsiI	ATGCAT	1 cut(s)	1422
NspI	RCATGY	3 cut(s)	638, 1329, 1603
NspV	TTCGAA	1 cut(s)	472
PaeR7I	CTCGAG	1 cut(s)	1384
PagI	TCATGA	2 cut(s)	790, 1432
PceI	AGGCCT	1 cut(s)	260
PctI	GAATGC	1 cut(s)	1364
PdmI	GAANNNNTTC	2 cut(s)	251, 1611
PfeI	GAWTC	4 cut(s)	247, 509, 652, 1668
PinAI	ACCGGT	3 cut(s)	83, 1151, 1377
PkrI	GCNGC	6 cut(s)	325, 700, 1007, 1480, 1483, 1822
Ple19I	CGATCG	1 cut(s)	313
PleI	GAGTC	2 cut(s)	356, 1395
PpsI	GAGTC	2 cut(s)	356, 1395
PshAI	GACNNNNGTC	1 cut(s)	347
PsiI	TTATAA	1 cut(s)	1020
Psp124BI	GAGCTC	1 cut(s)	384
Psp6I	CCWGG	4 cut(s)	235, 1100, 1234, 1618
PspGI	CCWGG	4 cut(s)	235, 1100, 1234, 1618
PspN4I	GGNNCC	4 cut(s)	1060, 1353, 1624, 1798
PspPI	GGNCC	2 cut(s)	601, 1375
PspXI	VCTCGAGB	1 cut(s)	1384
PstI	CTGCAG	1 cut(s)	1319
PstNI	CAGNNNCTG	1 cut(s)	1498
PsuI	RGATCY	1 cut(s)	1303
PvuI	CGATCG	1 cut(s)	313
PvuII	CAGCTG	1 cut(s)	1169
RseI	CAYNNNNRTG	2 cut(s)	170, 1404
SacI	GAGCTC	1 cut(s)	384
SaqAI	TTAA	3 cut(s)	80, 648, 1185
SatI	GCNGC	6 cut(s)	324, 699, 1006, 1479, 1482, 1821
Sau3AI	GATC	9 cut(s)	310, 427, 787, 795, 1303, 1396, 1567, 1657, 1683
Sau96I	GGNCC	2 cut(s)	601, 1375
SchI	GAGTC	2 cut(s)	357, 1396
ScrFI	CCNGG	4 cut(s)	237, 1102, 1236, 1620
SduI	GDGCHC	3 cut(s)	384, 1108, 1385
SfaNI	GCATC	6 cut(s)	414, 732, 1164, 1402, 1710, 1816
SfcI	CTRYAG	2 cut(s)	810, 1315
Sfr274I	CTCGAG	1 cut(s)	1384
SfuI	TTCGAA	1 cut(s)	472
SinI	GGWCC	2 cut(s)	601, 1375
SlaI	CTCGAG	1 cut(s)	1384
SmiMI	CAYNNNNRTG	2 cut(s)	170, 1404
SmlI	CTYRAG	3 cut(s)	647, 1384, 1724
SmoI	CTYRAG	3 cut(s)	647, 1384, 1724
Sse9I	AATT	7 cut(s)	30, 287, 452, 1082, 1226, 1284, 1297
SseBI	AGGCCT	1 cut(s)	260
SsiI	CCGC	7 cut(s)	47, 162, 210, 533, 698, 1820, 1837
SspMI	CTAG	2 cut(s)	152, 488
SstI	GAGCTC	1 cut(s)	384
StuI	AGGCCT	1 cut(s)	260
StyD4I	CCNGG	4 cut(s)	235, 1100, 1234, 1618
TaaI	ACNGT	6 cut(s)	605, 851, 963, 1426, 1579, 1591
TaiI	ACGT	5 cut(s)	93, 297, 580, 841, 953
TaqI	TCGA	6 cut(s)	255, 263, 408, 440, 472, 1385
TasI	AATT	7 cut(s)	30, 287, 452, 1082, 1226, 1284, 1297
TauI	GCSGC	2 cut(s)	701, 1823
TfiI	GAWTC	4 cut(s)	247, 509, 652, 1668
Tru1I	TTAA	3 cut(s)	80, 648, 1185
Tru9I	TTAA	3 cut(s)	80, 648, 1185
TscAI	CASTG	3 cut(s)	1102, 1519, 1642
TseFI	GTSAC	3 cut(s)	111, 671, 1046
TseI	GCWGC	4 cut(s)	323, 1005, 1478, 1481
Tsp45I	GTSAC	3 cut(s)	111, 671, 1046
TspDTI	ATGAA	2 cut(s)	64, 1495
TspRI	CASTG	3 cut(s)	1102, 1519, 1642
Vha464I	CTTAAG	1 cut(s)	647
VpaK11BI	GGWCC	2 cut(s)	601, 1375
XagI	CCTNNNNNAGG	1 cut(s)	1123
XapI	RAATTY	4 cut(s)	287, 1226, 1284, 1297
XceI	RCATGY	3 cut(s)	638, 1329, 1603
XhoI	CTCGAG	1 cut(s)	1384
XmnI	GAANNNNTTC	2 cut(s)	251, 1611
XspI	CTAG	2 cut(s)	152, 488
Zsp2I	ATGCAT	1 cut(s)	1422

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.